Spo27000 (gene)

Overview
NameSpo27000
Typegene
OrganismSpinacia oleracea (Spinach)
DescriptionUnknown protein
LocationSpoScf_00361 : 52092 .. 52481 (-)
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCAATTATTTGTGAAGAAGAAGAAGAAGAAGAAGCACAGTCAACACCAAAATGTGGCATGATGAAGGTGCAACTCGTTTCGAAATCAAAAAGTGAGAGGCTCTTAGAGAAGTATTTTGACGCGTTCGAGTTCGAGTTCGAGTTCGATTATGAGCAAAGCGGGATATGGTCGCCCCCAGTGAGACGTACCGCGTACGTTGGTTCGCCCGGGAGGATGTTTACGGAACAAGAAATGATGGATAGGCTTAAGTGTGTTTTGGAAGGTAGGAATGGTAGTTGTAGAAGAACCGGGTCTGCTTGTTTAGATGTTTGGGGTTGTATTTCTTCTTTGTTTTCTACTTTCCAGAAAAGGGTCAAGAAAAGAAGAGAAATTACACTTGGTATGTGA

mRNA sequence

ATGTCAATTATTTGTGAAGAAGAAGAAGAAGAAGAAGCACAGTCAACACCAAAATGTGGCATGATGAAGGTGCAACTCGTTTCGAAATCAAAAAGTGAGAGGCTCTTAGAGAAGTATTTTGACGCGTTCGAGTTCGAGTTCGAGTTCGATTATGAGCAAAGCGGGATATGGTCGCCCCCAGTGAGACGTACCGCGTACGTTGGTTCGCCCGGGAGGATGTTTACGGAACAAGAAATGATGGATAGGCTTAAGTGTGTTTTGGAAGGTAGGAATGGTAGTTGTAGAAGAACCGGGTCTGCTTGTTTAGATGTTTGGGGTTGTATTTCTTCTTTGTTTTCTACTTTCCAGAAAAGGGTCAAGAAAAGAAGAGAAATTACACTTGGTATGTGA

Coding sequence (CDS)

ATGTCAATTATTTGTGAAGAAGAAGAAGAAGAAGAAGCACAGTCAACACCAAAATGTGGCATGATGAAGGTGCAACTCGTTTCGAAATCAAAAAGTGAGAGGCTCTTAGAGAAGTATTTTGACGCGTTCGAGTTCGAGTTCGAGTTCGATTATGAGCAAAGCGGGATATGGTCGCCCCCAGTGAGACGTACCGCGTACGTTGGTTCGCCCGGGAGGATGTTTACGGAACAAGAAATGATGGATAGGCTTAAGTGTGTTTTGGAAGGTAGGAATGGTAGTTGTAGAAGAACCGGGTCTGCTTGTTTAGATGTTTGGGGTTGTATTTCTTCTTTGTTTTCTACTTTCCAGAAAAGGGTCAAGAAAAGAAGAGAAATTACACTTGGTATGTGA

Protein sequence

MSIICEEEEEEEAQSTPKCGMMKVQLVSKSKSERLLEKYFDAFEFEFEFDYEQSGIWSPPVRRTAYVGSPGRMFTEQEMMDRLKCVLEGRNGSCRRTGSACLDVWGCISSLFSTFQKRVKKRREITLGM
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Spo27000.1Spo27000.1mRNA


Homology
[Close]
BLAST of Spo27000.1 vs. NCBI nr
Match: gi|870852535|gb|KMT04450.1| (hypothetical protein BVRB_8g181220 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 194.9 bits (494), Expect = 8.700e-47
Identity = 98/126 (77.78%), Postives = 109/126 (86.51%), Query Frame = 1 

		  

Query: 4   ICEEEEEEEAQSTPKCGMMKVQLVSKSKSERLLEKYFDAFEFEFEFDYEQSGIWSPPVRR 63
           ICEE+ +    STP+CGM K+QLVSKSKSERLLEKYFDA  FEF+F+YE+SGIWSPPVRR
Sbjct: 3   ICEEDVQVST-STPQCGM-KIQLVSKSKSERLLEKYFDA--FEFDFNYEESGIWSPPVRR 62

Query: 64  TAYVGSPGRMFTEQEMMDRLKCVLEGRNGSCRRTGSACLDVWGCISSLFSTFQKRVKKRR 123
           T YVGSPGR FTE+EMM +L+ VLE RNGSC R  SACLDVWGCISSLFSTFQKRVK+RR
Sbjct: 63  TTYVGSPGRCFTEEEMMAKLRDVLEARNGSCTRRNSACLDVWGCISSLFSTFQKRVKRRR 122

Query: 124 EITLGM 130
           EIT GM
Sbjct: 123 EITFGM 124
[Close]
BLAST of Spo27000.1 vs. NCBI nr
Match: gi|731349918|ref|XP_010686243.1| (PREDICTED: uncharacterized protein LOC104900517 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 193.0 bits (489), Expect = 3.300e-46
Identity = 97/125 (77.60%), Postives = 108/125 (86.40%), Query Frame = 1 

		  

Query: 4   ICEEEEEEEAQSTPKCGMMKVQLVSKSKSERLLEKYFDAFEFEFEFDYEQSGIWSPPVRR 63
           ICEE+ +    STP+CGM K+QLVSKSKSERLLEKYFDA  FEF+F+YE+SGIWSPPVRR
Sbjct: 3   ICEEDVQVST-STPQCGM-KIQLVSKSKSERLLEKYFDA--FEFDFNYEESGIWSPPVRR 62

Query: 64  TAYVGSPGRMFTEQEMMDRLKCVLEGRNGSCRRTGSACLDVWGCISSLFSTFQKRVKKRR 123
           T YVGSPGR FTE+EMM +L+ VLE RNGSC R  SACLDVWGCISSLFSTFQKRVK+RR
Sbjct: 63  TTYVGSPGRCFTEEEMMAKLRDVLEARNGSCTRRNSACLDVWGCISSLFSTFQKRVKRRR 122

Query: 124 EITLG 129
           EIT G
Sbjct: 123 EITFG 123
[Close]
BLAST of Spo27000.1 vs. NCBI nr
Match: gi|743911838|ref|XP_010999783.1| (PREDICTED: uncharacterized protein LOC105107528 [Populus euphratica])

HSP 1 Score: 102.8 bits (255), Expect = 4.500e-19
Identity = 50/94 (53.19%), Postives = 72/94 (76.60%), Query Frame = 1 

		  

Query: 12  EAQSTPKCGMMKVQLVSKSKSERLLEKYFDAFEFEFEFDYEQSGIWSPPVRRTAYVGSPG 71
           E+ S+PK    ++Q+VSK  S+RLL+K+FDA E+  +FDYEQSGIWSPP+RR+A++ SPG
Sbjct: 14  ESSSSPK----RIQIVSKFVSDRLLDKFFDATEY--DFDYEQSGIWSPPIRRSAFLSSPG 73

Query: 72  RMFTEQEMMDRLKCVLEGRNGSCRRTGSACLDVW 106
           R+FTE+EM+++L  V++ R    RR   AC +VW
Sbjct: 74  RIFTEEEMLEKLGNVMDARR--ARRHNKACCNVW 99
[Close]
BLAST of Spo27000.1 vs. NCBI nr
Match: gi|1000958783|ref|XP_015576682.1| (PREDICTED: uncharacterized protein LOC8275171 [Ricinus communis])

HSP 1 Score: 100.9 bits (250), Expect = 1.700e-18
Identity = 46/87 (52.87%), Postives = 68/87 (78.16%), Query Frame = 1 

		  

Query: 23  KVQLVSKSKSERLLEKYFDAFEFEFEFDYEQSGIWSPPVRRTAYVGSPGRMFTEQEMMDR 82
           K+QLVSKS S+RLL+K+FD  EF  +FDY+QSGIWSPP++R+AY+ SPG++FTE+EM+ +
Sbjct: 14  KIQLVSKSVSDRLLDKFFDVSEF--DFDYQQSGIWSPPIKRSAYLSSPGQIFTEEEMLKK 73

Query: 83  LKCVLEGRNGSCRRTGSACLDVWGCIS 110
           L+ +++ R G   R   A  +VW C++
Sbjct: 74  LRNIMDERRG---RRHKAFRNVWQCVA 95
[Close]
BLAST of Spo27000.1 vs. NCBI nr
Match: gi|1009106637|ref|XP_015875522.1| (PREDICTED: uncharacterized protein LOC107412288 [Ziziphus jujuba])

HSP 1 Score: 100.5 bits (249), Expect = 2.200e-18
Identity = 46/74 (62.16%), Postives = 63/74 (85.14%), Query Frame = 1 

		  

Query: 22  MKVQLVSKSKSERLLEKYFDAFEFEFEFDYEQSGIWSPPVRRTAYVGSPGRMFTEQEMMD 81
           +KVQLVSKS S++LLEK+FDA EF  +FDYEQSG+WSPPVRR+ ++ SPG++FTEQEM++
Sbjct: 51  LKVQLVSKSTSDQLLEKFFDAAEF--DFDYEQSGLWSPPVRRSVFMSSPGKIFTEQEMLE 110

Query: 82  RLKCVLEGRNGSCR 96
           RLK +++ R+   R
Sbjct: 111 RLKTMMDARDSGRR 122
[Close]
BLAST of Spo27000.1 vs. UniProtKB/TrEMBL
Match: A0A0J8BSE7_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_8g181220 PE=4 SV=1)

HSP 1 Score: 194.9 bits (494), Expect = 6.100e-47
Identity = 98/126 (77.78%), Postives = 109/126 (86.51%), Query Frame = 1 

		  

Query: 4   ICEEEEEEEAQSTPKCGMMKVQLVSKSKSERLLEKYFDAFEFEFEFDYEQSGIWSPPVRR 63
           ICEE+ +    STP+CGM K+QLVSKSKSERLLEKYFDA  FEF+F+YE+SGIWSPPVRR
Sbjct: 3   ICEEDVQVST-STPQCGM-KIQLVSKSKSERLLEKYFDA--FEFDFNYEESGIWSPPVRR 62

Query: 64  TAYVGSPGRMFTEQEMMDRLKCVLEGRNGSCRRTGSACLDVWGCISSLFSTFQKRVKKRR 123
           T YVGSPGR FTE+EMM +L+ VLE RNGSC R  SACLDVWGCISSLFSTFQKRVK+RR
Sbjct: 63  TTYVGSPGRCFTEEEMMAKLRDVLEARNGSCTRRNSACLDVWGCISSLFSTFQKRVKRRR 122

Query: 124 EITLGM 130
           EIT GM
Sbjct: 123 EITFGM 124
[Close]
BLAST of Spo27000.1 vs. UniProtKB/TrEMBL
Match: A0A061GEC9_THECC (Uncharacterized protein OS=Theobroma cacao GN=TCM_029650 PE=4 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 3.500e-18
Identity = 47/76 (61.84%), Postives = 63/76 (82.89%), Query Frame = 1 

		  

Query: 15 STPKCGMMKVQLVSKSKSERLLEKYFDAFEFEFEFDYEQSGIWSPPVRRTAYVGSPGRMF 74
          S P+   +KVQLVSKS S+RLLEK+FD  ++   FDYE+SG+WSPPVRR+A++ SPGR+F
Sbjct: 12 SLPERSPVKVQLVSKSVSDRLLEKFFDVSQY--NFDYEKSGLWSPPVRRSAFLSSPGRIF 71

Query: 75 TEQEMMDRLKCVLEGR 91
          TEQEM++RLK V++ R
Sbjct: 72 TEQEMLERLKSVMDRR 85
[Close]
BLAST of Spo27000.1 vs. UniProtKB/TrEMBL
Match: B9H2F1_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0004s05950g PE=4 SV=2)

HSP 1 Score: 99.4 bits (246), Expect = 3.500e-18
Identity = 51/96 (53.12%), Postives = 71/96 (73.96%), Query Frame = 1 

		  

Query: 12  EAQSTPKCGMMKVQLVSKSKSERLLEKYFDAFEFEFEFDYEQSGIWSPPVRRTAYVGSPG 71
           E+ S+PK    ++Q VSK  S+RLL+K+FDA EF  +FDYEQSGIWSPP+RR+A++ SPG
Sbjct: 14  ESSSSPK----RIQRVSKFVSDRLLDKFFDATEF--DFDYEQSGIWSPPIRRSAFLSSPG 73

Query: 72  RMFTEQEMMDRLKCVLEGRNGSCRRTGSACLDVWGC 108
           R+FTE+EM+++L  V++ R    RR   AC +V  C
Sbjct: 74  RIFTEEEMLEKLGNVMDARR--ARRHNKACCNVVCC 101
[Close]
BLAST of Spo27000.1 vs. UniProtKB/TrEMBL
Match: U5FVR3_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0011s06960g PE=4 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 5.900e-18
Identity = 48/91 (52.75%), Postives = 67/91 (73.63%), Query Frame = 1 

		  

Query: 17  PKCGMMKVQLVSKSKSERLLEKYFDAFEFEFEFDYEQSGIWSPPVRRTAYVGSPGRMFTE 76
           P     K+QLVSK  S+RLL+K+FDA EF  +FDYEQSG+WSPP+RR+A++ SPGR+FTE
Sbjct: 20  PSSSPKKIQLVSKFVSDRLLDKFFDASEF--DFDYEQSGLWSPPIRRSAFLSSPGRIFTE 79

Query: 77  QEMMDRLKCVLEGRNGSCRRTGSACLDVWGC 108
           +EM+ RL+ V++ R  +  +   AC +V  C
Sbjct: 80  EEMLGRLRKVMDARRDTRHK---ACWNVVWC 105
[Close]
BLAST of Spo27000.1 vs. UniProtKB/TrEMBL
Match: B9S8D0_RICCO (Putative uncharacterized protein OS=Ricinus communis GN=RCOM_1251020 PE=4 SV=1)

HSP 1 Score: 94.4 bits (233), Expect = 1.100e-16
Identity = 41/70 (58.57%), Postives = 60/70 (85.71%), Query Frame = 1 

		  

Query: 23 KVQLVSKSKSERLLEKYFDAFEFEFEFDYEQSGIWSPPVRRTAYVGSPGRMFTEQEMMDR 82
          K+QLVSKS S+RLL+K+FD  EF  +FDY+QSGIWSPP++R+AY+ SPG++FTE+EM+ +
Sbjct: 14 KIQLVSKSVSDRLLDKFFDVSEF--DFDYQQSGIWSPPIKRSAYLSSPGQIFTEEEMLKK 73

Query: 83 LKCVLEGRNG 93
          L+ +++ R G
Sbjct: 74 LRNIMDERRG 81
The following BLAST results are available for this feature:
BLAST of Spo27000.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5
Match NameE-valueIdentityDescription
gi|870852535|gb|KMT04450.1|8.7e-4777.7hypothetical protein BVRB_8g18... [more]
gi|731349918|ref|XP_010686243.1|3.3e-4677.6PREDICTED: uncharacterized pro... [more]
gi|743911838|ref|XP_010999783.1|4.5e-1953.1PREDICTED: uncharacterized pro... [more]
gi|1000958783|ref|XP_015576682.1|1.7e-1852.8PREDICTED: uncharacterized pro... [more]
gi|1009106637|ref|XP_015875522.1|2.2e-1862.1PREDICTED: uncharacterized pro... [more]
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BLAST of Spo27000.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0J8BSE7_BETVU6.1e-4777.7Uncharacterized protein OS=Bet... [more]
A0A061GEC9_THECC3.5e-1861.8Uncharacterized protein OS=The... [more]
B9H2F1_POPTR3.5e-1853.1Uncharacterized protein OS=Pop... [more]
U5FVR3_POPTR5.9e-1852.7Uncharacterized protein OS=Pop... [more]
B9S8D0_RICCO1.1e-1658.5Putative uncharacterized prote... [more]
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BLAST of Spo27000.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Spo27000.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 0
Match NameE-valueIdentityDescription
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InterPro
Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR34287FAMILY NOT NAMEDcoord: 22..84
score: 3.2
NoneNo IPR availablePANTHERPTHR34287:SF2SUBFAMILY NOT NAMEDcoord: 22..84
score: 3.2

GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0009069 serine family amino acid metabolic process
biological_process GO:0006468 protein phosphorylation
biological_process GO:0046786 viral replication complex formation and maintenance
biological_process GO:0010200 response to chitin
biological_process GO:0061416 regulation of transcription from RNA polymerase II promoter in response to salt stress
biological_process GO:0048193 Golgi vesicle transport
biological_process GO:0006470 protein dephosphorylation
cellular_component GO:0005774 vacuolar membrane
cellular_component GO:0008287 protein serine/threonine phosphatase complex
cellular_component GO:0005634 nucleus
cellular_component GO:0005737 cytoplasm
cellular_component GO:0009506 plasmodesma
cellular_component GO:0005886 plasma membrane
cellular_component GO:0009941 chloroplast envelope
cellular_component GO:0016020 membrane
cellular_component GO:0009705 plant-type vacuole membrane
cellular_component GO:0005739 mitochondrion
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0030246 carbohydrate binding
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0005515 protein binding
molecular_function GO:0046872 metal ion binding
molecular_function GO:0004722 protein serine/threonine phosphatase activity
molecular_function GO:0008289 lipid binding
molecular_function GO:0016829 lyase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
RNA-Seq Expression