Spo28094 (gene) | SpinachBase

Overview


Name	Spo28094
Type	gene
Organism	Spinacia oleracea (Spinach)
Description	Kunitz trypsin inhibitor
Location	chr1 : 47366994 .. 47367713 (-)

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTCACCACCTTCTTCTTCCAGCTGCAACCCTCCTCTTAACCCTCCTCTTTCTCTCTCCTCCCACGGCCACGGCCAAGGACTCGGTAGTCCTAGACGTGGACGGGAACCCAGTAGAAGCAGGGTCACAATACTACGTCCTCACTTCTGGATGGGGTGGAGCAGGCCGTGGTGGCCTAACCGCTGCATCACTCGGTGGCCCGTGCCCTTTATACGTAGGTCAATATAGGCTATGGGTTCAAAAGGGCAGCCCAGTAACATTCTACCCTTCCAACCCTTCACAAAAACACATCACACAAAGTTCAGACGTCAACATTGCTTTCGCCCCAAACCCAATCTGCAGAAACCAAGGGGTTTGGCAGCTGGGTTACAATGGCATTTCATTCTACATAACTACTAATGGAGTTATTGGAAAGCAAGAAGATGTCGACACATTCGCCAATTGGTTCAAGATCGAACAAGCAGGAGGGTTACCAGGAGCATACAAGATAGTGTATTGTTTCGCCGTACCAAGTACTGGTCCTGAACCTGTTGTTCCTGATAGTCGTCGTCGTACTGGTCCTGAACCTGATACTGAGTATTGTGAACAGTTGGATGCTACTGTACCCGTGCCGCCGATGGGTTTGTCGTTGTTAACTCCGGTACCCATTGATAATCCTCTACCCTTCTTTGGCTTTGTATTCAAGAAGGTTAACACTACTGCCAATCATAGCTACTAG

mRNA sequence

ATGGCTCACCACCTTCTTCTTCCAGCTGCAACCCTCCTCTTAACCCTCCTCTTTCTCTCTCCTCCCACGGCCACGGCCAAGGACTCGGTAGTCCTAGACGTGGACGGGAACCCAGTAGAAGCAGGGTCACAATACTACGTCCTCACTTCTGGATGGGGTGGAGCAGGCCGTGGTGGCCTAACCGCTGCATCACTCGGTGGCCCGTGCCCTTTATACGTAGGTCAATATAGGCTATGGGTTCAAAAGGGCAGCCCAGTAACATTCTACCCTTCCAACCCTTCACAAAAACACATCACACAAAGTTCAGACGTCAACATTGCTTTCGCCCCAAACCCAATCTGCAGAAACCAAGGGGTTTGGCAGCTGGGTTACAATGGCATTTCATTCTACATAACTACTAATGGAGTTATTGGAAAGCAAGAAGATGTCGACACATTCGCCAATTGGTTCAAGATCGAACAAGCAGGAGGGTTACCAGGAGCATACAAGATAGTGTATTGTTTCGCCGTACCAAGTACTGGTCCTGAACCTGTTGTTCCTGATAGTCGTCGTCGTACTGGTCCTGAACCTGATACTGAGTATTGTGAACAGTTGGATGCTACTGTACCCGTGCCGCCGATGGGTTTGTCGTTGTTAACTCCGGTACCCATTGATAATCCTCTACCCTTCTTTGGCTTTGTATTCAAGAAGGTTAACACTACTGCCAATCATAGCTACTAG

Coding sequence (CDS)

ATGGCTCACCACCTTCTTCTTCCAGCTGCAACCCTCCTCTTAACCCTCCTCTTTCTCTCTCCTCCCACGGCCACGGCCAAGGACTCGGTAGTCCTAGACGTGGACGGGAACCCAGTAGAAGCAGGGTCACAATACTACGTCCTCACTTCTGGATGGGGTGGAGCAGGCCGTGGTGGCCTAACCGCTGCATCACTCGGTGGCCCGTGCCCTTTATACGTAGGTCAATATAGGCTATGGGTTCAAAAGGGCAGCCCAGTAACATTCTACCCTTCCAACCCTTCACAAAAACACATCACACAAAGTTCAGACGTCAACATTGCTTTCGCCCCAAACCCAATCTGCAGAAACCAAGGGGTTTGGCAGCTGGGTTACAATGGCATTTCATTCTACATAACTACTAATGGAGTTATTGGAAAGCAAGAAGATGTCGACACATTCGCCAATTGGTTCAAGATCGAACAAGCAGGAGGGTTACCAGGAGCATACAAGATAGTGTATTGTTTCGCCGTACCAAGTACTGGTCCTGAACCTGTTGTTCCTGATAGTCGTCGTCGTACTGGTCCTGAACCTGATACTGAGTATTGTGAACAGTTGGATGCTACTGTACCCGTGCCGCCGATGGGTTTGTCGTTGTTAACTCCGGTACCCATTGATAATCCTCTACCCTTCTTTGGCTTTGTATTCAAGAAGGTTAACACTACTGCCAATCATAGCTACTAG

Protein sequence

MAHHLLLPAATLLLTLLFLSPPTATAKDSVVLDVDGNPVEAGSQYYVLTSGWGGAGRGGLTAASLGGPCPLYVGQYRLWVQKGSPVTFYPSNPSQKHITQSSDVNIAFAPNPICRNQGVWQLGYNGISFYITTNGVIGKQEDVDTFANWFKIEQAGGLPGAYKIVYCFAVPSTGPEPVVPDSRRRTGPEPDTEYCEQLDATVPVPPMGLSLLTPVPIDNPLPFFGFVFKKVNTTANHSY

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spo28094.1	Spo28094.1	mRNA

Homology

[Close]

BLAST of Spo28094.1 vs. NCBI nr
Match: gi|902228865|gb|KNA21296.1| (hypothetical protein SOVF_044550 [Spinacia oleracea])

HSP 1 Score: 496.5 bits (1277), Expect = 2.600e-137
Identity = 239/239 (100.00%), Postives = 239/239 (100.00%), Query Frame = 1


		  
Query: 1   MAHHLLLPAATLLLTLLFLSPPTATAKDSVVLDVDGNPVEAGSQYYVLTSGWGGAGRGGL 60
           MAHHLLLPAATLLLTLLFLSPPTATAKDSVVLDVDGNPVEAGSQYYVLTSGWGGAGRGGL
Sbjct: 1   MAHHLLLPAATLLLTLLFLSPPTATAKDSVVLDVDGNPVEAGSQYYVLTSGWGGAGRGGL 60

Query: 61  TAASLGGPCPLYVGQYRLWVQKGSPVTFYPSNPSQKHITQSSDVNIAFAPNPICRNQGVW 120
           TAASLGGPCPLYVGQYRLWVQKGSPVTFYPSNPSQKHITQSSDVNIAFAPNPICRNQGVW
Sbjct: 61  TAASLGGPCPLYVGQYRLWVQKGSPVTFYPSNPSQKHITQSSDVNIAFAPNPICRNQGVW 120

Query: 121 QLGYNGISFYITTNGVIGKQEDVDTFANWFKIEQAGGLPGAYKIVYCFAVPSTGPEPVVP 180
           QLGYNGISFYITTNGVIGKQEDVDTFANWFKIEQAGGLPGAYKIVYCFAVPSTGPEPVVP
Sbjct: 121 QLGYNGISFYITTNGVIGKQEDVDTFANWFKIEQAGGLPGAYKIVYCFAVPSTGPEPVVP 180

Query: 181 DSRRRTGPEPDTEYCEQLDATVPVPPMGLSLLTPVPIDNPLPFFGFVFKKVNTTANHSY 240
           DSRRRTGPEPDTEYCEQLDATVPVPPMGLSLLTPVPIDNPLPFFGFVFKKVNTTANHSY
Sbjct: 181 DSRRRTGPEPDTEYCEQLDATVPVPPMGLSLLTPVPIDNPLPFFGFVFKKVNTTANHSY 239

[Close]

BLAST of Spo28094.1 vs. NCBI nr
Match: gi|902188089|gb|KNA11068.1| (hypothetical protein SOVF_138590 [Spinacia oleracea])

HSP 1 Score: 336.7 bits (862), Expect = 3.400e-89
Identity = 169/235 (71.91%), Postives = 190/235 (80.85%), Query Frame = 1


		  
Query: 1   MAHHLLLPAATLLLTLLFLSPPTATAKDSVVLDVDGNPVEAGSQYYVLTSGWGGAGRGGL 60
           MA+HLLLPAAT++LTLLFLSPPT TA DSVVLD+DGNP+EAGSQYYVLT G  GAGRGGL
Sbjct: 1   MANHLLLPAATIILTLLFLSPPTVTAADSVVLDMDGNPLEAGSQYYVLTLGTRGAGRGGL 60

Query: 61  TAASLGGPCPLYVGQYRLWVQKGSPVTFYPSNPSQKHITQSSDVNIAFAPNPICRNQGVW 120
           TA  L GPCPLYVGQ+R W Q+GSPVTFYP++ SQK+ITQSSDVNIAF P+PICRNQGVW
Sbjct: 61  TAERLTGPCPLYVGQHRSWTQQGSPVTFYPADSSQKNITQSSDVNIAFNPSPICRNQGVW 120

Query: 121 QLGYNGIS-FYITTNGVIGKQEDVDTFANWFKIEQAGGLPGAYKIVYCFAVPSTGPEPVV 180
           QL  NG+  F++TTNGVIG     +TF NWFKIE+A   P +YKIVYCF VP  GP P  
Sbjct: 121 QLSSNGLGVFHVTTNGVIG-NPGAETFTNWFKIEKASSEPDSYKIVYCFVVPGPGPVPAP 180

Query: 181 PDSRRRTGPEPDTEYCEQLDATVPVPPMGLSLLTPVPIDNPLPFFGFVFKKVNTT 235
           P+    TG     EYCEQLDAT+PVPP+  SLL+ VPIDNPLPFFGFVFKKV TT
Sbjct: 181 PE----TGKNRPDEYCEQLDATIPVPPLDFSLLSLVPIDNPLPFFGFVFKKVTTT 230

[Close]

BLAST of Spo28094.1 vs. NCBI nr
Match: gi|902228866|gb|KNA21297.1| (hypothetical protein SOVF_044560 [Spinacia oleracea])

HSP 1 Score: 302.4 bits (773), Expect = 7.200e-79
Identity = 169/245 (68.98%), Postives = 191/245 (77.96%), Query Frame = 1


		  
Query: 1   MAHHLLLPAATLLLTLLFLSPPTATAK--DSVVLDVDGNPVEAGSQYYVLTSGWGGAGRG 60
           MA+HLLLPAAT+ LTLLFLSP TATA   DSVV+D+DGNP+EAGSQYYVLTSG  GAGRG
Sbjct: 1   MANHLLLPAATIFLTLLFLSPATATATAADSVVVDIDGNPLEAGSQYYVLTSGSRGAGRG 60

Query: 61  GLTAASLGGPCPLYVGQYRLWVQKGSPVTFYPSNPSQKHITQSSDVNIAFAPNPICRNQG 120
           GL A  LGG CPLYVGQ+R W  +GSPV+FYP++ SQK+IT SSDVNIAF  + +CRNQG
Sbjct: 61  GLNAVPLGGICPLYVGQHRSWNNRGSPVSFYPADLSQKNITLSSDVNIAFGLSAMCRNQG 120

Query: 121 VWQLGYNGIS-FYITTNGVIGKQEDVDTFANWFKIEQAGGLP----GAYKIVYCFAVPST 180
           VWQL  N +  FYITTNG IG     +TF NWFKIE+   LP     +Y+IVYCFAVP  
Sbjct: 121 VWQLSSNELGVFYITTNGEIGNP-GAETFTNWFKIEK---LPFPKSNSYRIVYCFAVPKP 180

Query: 181 GPEPV--VPDSRRRTGPEP--DTEYCEQLDATVPVPPMGLSLLTPVPIDNPLPFFGFVFK 235
           GPEPV  VPDSRR TGPE    TEYC+QLDAT+P  P  LS+L+ VPIDNPLPFFGFVFK
Sbjct: 181 GPEPVHVVPDSRR-TGPESVVPTEYCQQLDATLP-GPWDLSMLSLVPIDNPLPFFGFVFK 239

[Close]

BLAST of Spo28094.1 vs. NCBI nr
Match: gi|902188088|gb|KNA11067.1| (hypothetical protein SOVF_138580 [Spinacia oleracea])

HSP 1 Score: 297.7 bits (761), Expect = 1.800e-77
Identity = 162/236 (68.64%), Postives = 181/236 (76.69%), Query Frame = 1


		  
Query: 1   MAHHLLLPAATLLLTLLFLSPPTATAKDSVVLDVDGNPVEAGSQYYVLTSGWGGAGRGGL 60
           MAHHLLL AAT+LLTL FLSPPTA   DSVVLD+DGNPVEAGSQYYVLT G  GAGRGGL
Sbjct: 1   MAHHLLLAAATILLTLFFLSPPTAA--DSVVLDIDGNPVEAGSQYYVLTRGTRGAGRGGL 60

Query: 61  TAASLGGPCPLYVGQYRLWVQKGSPVTFYPSNPSQKHITQSSDVNIAFAPNPICRNQGVW 120
           TAA LGG CPLYVGQ+R W Q+GSPVTF+P+NPSQK+ITQSSDVNIAF  NPICRN GVW
Sbjct: 61  TAAPLGGLCPLYVGQHRSWTQQGSPVTFFPANPSQKYITQSSDVNIAFGLNPICRNLGVW 120

Query: 121 QLGY--NGISFYITTNGVIGKQEDVDTFANWFKIEQAGGLPGAYKIVYCFAVPSTGPEPV 180
           QLGY  +G+  Y+TTNGVIG     +TF NWFKIE A G P +YKIVYCF VP+     +
Sbjct: 121 QLGYSFSGVP-YVTTNGVIG-NPGAETFTNWFKIEHAFG-PESYKIVYCFLVPAPWYSRM 180

Query: 181 VPDSRRRTGPEPDTEYCEQLDATVPVPPMGLSLLTPVPIDNPLPFFGFVFKKVNTT 235
            P            EYCEQLDAT+P  P  L++L+ VPID PLPFFGFVFKKV +T
Sbjct: 181 SP-----------YEYCEQLDATIP-GPSDLAMLSLVPIDKPLPFFGFVFKKVTST 219

[Close]

BLAST of Spo28094.1 vs. NCBI nr
Match: gi|902078036|gb|KNA03096.1| (hypothetical protein SOVF_212410 [Spinacia oleracea])

HSP 1 Score: 240.0 bits (611), Expect = 4.400e-60
Identity = 135/216 (62.50%), Postives = 156/216 (72.22%), Query Frame = 1


		  
Query: 1   MAHHLLLPAATLLLTLLFLSPPTATAKDSVVLDVDGNPVEAGSQYYVLTSGWGGAGRGGL 60
           MA+HLLL AAT+L    FLSPPT TA DSVVLD+DGNPVEAGS+YYV T G  GA RGGL
Sbjct: 1   MANHLLLLAATIL----FLSPPTVTAADSVVLDMDGNPVEAGSRYYVQTLGSRGASRGGL 60

Query: 61  TAASLGGPCPLYVGQYRLWVQKGSPVTFYPSNPSQKHITQSSDVNIAFAPNPICRNQGVW 120
            A  L G CPLYVGQ+R W  +G P+TFYP++PSQK+IT SSDVNIAF  N +CRNQGVW
Sbjct: 61  AAEPLSGICPLYVGQHRSWTNQGRPITFYPADPSQKNITLSSDVNIAFGLNGLCRNQGVW 120

Query: 121 QLGYNGIS-FYITTNGVIGKQEDVDTFANWFKIEQAGGLPG--AYKIVYCFAVPSTGPEP 180
           QL  NG   FY+TTNG IG     +TF NWFK+E+A  LPG  +YKIVYCF VP      
Sbjct: 121 QLTSNGDGVFYVTTNGEIG-NPGAETFTNWFKLEEA--LPGTNSYKIVYCFLVP------ 180

Query: 181 VVPDSRRRTGPEPDTEYCEQLDATVPVPPMGLSLLT 214
            VP    ++   PD+EYCEQLDAT+P  P  LSLL+
Sbjct: 181 -VPAGNGKS--HPDSEYCEQLDATLP-GPWDLSLLS 199

[Close]

BLAST of Spo28094.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RP34_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_044550 PE=4 SV=1)

HSP 1 Score: 496.5 bits (1277), Expect = 1.800e-137
Identity = 239/239 (100.00%), Postives = 239/239 (100.00%), Query Frame = 1


		  
Query: 1   MAHHLLLPAATLLLTLLFLSPPTATAKDSVVLDVDGNPVEAGSQYYVLTSGWGGAGRGGL 60
           MAHHLLLPAATLLLTLLFLSPPTATAKDSVVLDVDGNPVEAGSQYYVLTSGWGGAGRGGL
Sbjct: 1   MAHHLLLPAATLLLTLLFLSPPTATAKDSVVLDVDGNPVEAGSQYYVLTSGWGGAGRGGL 60

Query: 61  TAASLGGPCPLYVGQYRLWVQKGSPVTFYPSNPSQKHITQSSDVNIAFAPNPICRNQGVW 120
           TAASLGGPCPLYVGQYRLWVQKGSPVTFYPSNPSQKHITQSSDVNIAFAPNPICRNQGVW
Sbjct: 61  TAASLGGPCPLYVGQYRLWVQKGSPVTFYPSNPSQKHITQSSDVNIAFAPNPICRNQGVW 120

Query: 121 QLGYNGISFYITTNGVIGKQEDVDTFANWFKIEQAGGLPGAYKIVYCFAVPSTGPEPVVP 180
           QLGYNGISFYITTNGVIGKQEDVDTFANWFKIEQAGGLPGAYKIVYCFAVPSTGPEPVVP
Sbjct: 121 QLGYNGISFYITTNGVIGKQEDVDTFANWFKIEQAGGLPGAYKIVYCFAVPSTGPEPVVP 180

Query: 181 DSRRRTGPEPDTEYCEQLDATVPVPPMGLSLLTPVPIDNPLPFFGFVFKKVNTTANHSY 240
           DSRRRTGPEPDTEYCEQLDATVPVPPMGLSLLTPVPIDNPLPFFGFVFKKVNTTANHSY
Sbjct: 181 DSRRRTGPEPDTEYCEQLDATVPVPPMGLSLLTPVPIDNPLPFFGFVFKKVNTTANHSY 239

[Close]

BLAST of Spo28094.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QWG7_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_138590 PE=4 SV=1)

HSP 1 Score: 336.7 bits (862), Expect = 2.400e-89
Identity = 169/235 (71.91%), Postives = 190/235 (80.85%), Query Frame = 1


		  
Query: 1   MAHHLLLPAATLLLTLLFLSPPTATAKDSVVLDVDGNPVEAGSQYYVLTSGWGGAGRGGL 60
           MA+HLLLPAAT++LTLLFLSPPT TA DSVVLD+DGNP+EAGSQYYVLT G  GAGRGGL
Sbjct: 1   MANHLLLPAATIILTLLFLSPPTVTAADSVVLDMDGNPLEAGSQYYVLTLGTRGAGRGGL 60

Query: 61  TAASLGGPCPLYVGQYRLWVQKGSPVTFYPSNPSQKHITQSSDVNIAFAPNPICRNQGVW 120
           TA  L GPCPLYVGQ+R W Q+GSPVTFYP++ SQK+ITQSSDVNIAF P+PICRNQGVW
Sbjct: 61  TAERLTGPCPLYVGQHRSWTQQGSPVTFYPADSSQKNITQSSDVNIAFNPSPICRNQGVW 120

Query: 121 QLGYNGIS-FYITTNGVIGKQEDVDTFANWFKIEQAGGLPGAYKIVYCFAVPSTGPEPVV 180
           QL  NG+  F++TTNGVIG     +TF NWFKIE+A   P +YKIVYCF VP  GP P  
Sbjct: 121 QLSSNGLGVFHVTTNGVIG-NPGAETFTNWFKIEKASSEPDSYKIVYCFVVPGPGPVPAP 180

Query: 181 PDSRRRTGPEPDTEYCEQLDATVPVPPMGLSLLTPVPIDNPLPFFGFVFKKVNTT 235
           P+    TG     EYCEQLDAT+PVPP+  SLL+ VPIDNPLPFFGFVFKKV TT
Sbjct: 181 PE----TGKNRPDEYCEQLDATIPVPPLDFSLLSLVPIDNPLPFFGFVFKKVTTT 230

[Close]

BLAST of Spo28094.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RR37_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_044560 PE=4 SV=1)

HSP 1 Score: 302.4 bits (773), Expect = 5.000e-79
Identity = 169/245 (68.98%), Postives = 191/245 (77.96%), Query Frame = 1


		  
Query: 1   MAHHLLLPAATLLLTLLFLSPPTATAK--DSVVLDVDGNPVEAGSQYYVLTSGWGGAGRG 60
           MA+HLLLPAAT+ LTLLFLSP TATA   DSVV+D+DGNP+EAGSQYYVLTSG  GAGRG
Sbjct: 1   MANHLLLPAATIFLTLLFLSPATATATAADSVVVDIDGNPLEAGSQYYVLTSGSRGAGRG 60

Query: 61  GLTAASLGGPCPLYVGQYRLWVQKGSPVTFYPSNPSQKHITQSSDVNIAFAPNPICRNQG 120
           GL A  LGG CPLYVGQ+R W  +GSPV+FYP++ SQK+IT SSDVNIAF  + +CRNQG
Sbjct: 61  GLNAVPLGGICPLYVGQHRSWNNRGSPVSFYPADLSQKNITLSSDVNIAFGLSAMCRNQG 120

Query: 121 VWQLGYNGIS-FYITTNGVIGKQEDVDTFANWFKIEQAGGLP----GAYKIVYCFAVPST 180
           VWQL  N +  FYITTNG IG     +TF NWFKIE+   LP     +Y+IVYCFAVP  
Sbjct: 121 VWQLSSNELGVFYITTNGEIGNP-GAETFTNWFKIEK---LPFPKSNSYRIVYCFAVPKP 180

Query: 181 GPEPV--VPDSRRRTGPEP--DTEYCEQLDATVPVPPMGLSLLTPVPIDNPLPFFGFVFK 235
           GPEPV  VPDSRR TGPE    TEYC+QLDAT+P  P  LS+L+ VPIDNPLPFFGFVFK
Sbjct: 181 GPEPVHVVPDSRR-TGPESVVPTEYCQQLDATLP-GPWDLSMLSLVPIDNPLPFFGFVFK 239

[Close]

BLAST of Spo28094.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QUV4_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_138580 PE=4 SV=1)

HSP 1 Score: 297.7 bits (761), Expect = 1.200e-77
Identity = 162/236 (68.64%), Postives = 181/236 (76.69%), Query Frame = 1


		  
Query: 1   MAHHLLLPAATLLLTLLFLSPPTATAKDSVVLDVDGNPVEAGSQYYVLTSGWGGAGRGGL 60
           MAHHLLL AAT+LLTL FLSPPTA   DSVVLD+DGNPVEAGSQYYVLT G  GAGRGGL
Sbjct: 1   MAHHLLLAAATILLTLFFLSPPTAA--DSVVLDIDGNPVEAGSQYYVLTRGTRGAGRGGL 60

Query: 61  TAASLGGPCPLYVGQYRLWVQKGSPVTFYPSNPSQKHITQSSDVNIAFAPNPICRNQGVW 120
           TAA LGG CPLYVGQ+R W Q+GSPVTF+P+NPSQK+ITQSSDVNIAF  NPICRN GVW
Sbjct: 61  TAAPLGGLCPLYVGQHRSWTQQGSPVTFFPANPSQKYITQSSDVNIAFGLNPICRNLGVW 120

Query: 121 QLGY--NGISFYITTNGVIGKQEDVDTFANWFKIEQAGGLPGAYKIVYCFAVPSTGPEPV 180
           QLGY  +G+  Y+TTNGVIG     +TF NWFKIE A G P +YKIVYCF VP+     +
Sbjct: 121 QLGYSFSGVP-YVTTNGVIG-NPGAETFTNWFKIEHAFG-PESYKIVYCFLVPAPWYSRM 180

Query: 181 VPDSRRRTGPEPDTEYCEQLDATVPVPPMGLSLLTPVPIDNPLPFFGFVFKKVNTT 235
            P            EYCEQLDAT+P  P  L++L+ VPID PLPFFGFVFKKV +T
Sbjct: 181 SP-----------YEYCEQLDATIP-GPSDLAMLSLVPIDKPLPFFGFVFKKVTST 219

[Close]

BLAST of Spo28094.1 vs. UniProtKB/TrEMBL
Match: A0A0K9Q9A5_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_212410 PE=4 SV=1)

HSP 1 Score: 240.0 bits (611), Expect = 3.000e-60
Identity = 135/216 (62.50%), Postives = 156/216 (72.22%), Query Frame = 1


		  
Query: 1   MAHHLLLPAATLLLTLLFLSPPTATAKDSVVLDVDGNPVEAGSQYYVLTSGWGGAGRGGL 60
           MA+HLLL AAT+L    FLSPPT TA DSVVLD+DGNPVEAGS+YYV T G  GA RGGL
Sbjct: 1   MANHLLLLAATIL----FLSPPTVTAADSVVLDMDGNPVEAGSRYYVQTLGSRGASRGGL 60

Query: 61  TAASLGGPCPLYVGQYRLWVQKGSPVTFYPSNPSQKHITQSSDVNIAFAPNPICRNQGVW 120
            A  L G CPLYVGQ+R W  +G P+TFYP++PSQK+IT SSDVNIAF  N +CRNQGVW
Sbjct: 61  AAEPLSGICPLYVGQHRSWTNQGRPITFYPADPSQKNITLSSDVNIAFGLNGLCRNQGVW 120

Query: 121 QLGYNGIS-FYITTNGVIGKQEDVDTFANWFKIEQAGGLPG--AYKIVYCFAVPSTGPEP 180
           QL  NG   FY+TTNG IG     +TF NWFK+E+A  LPG  +YKIVYCF VP      
Sbjct: 121 QLTSNGDGVFYVTTNGEIG-NPGAETFTNWFKLEEA--LPGTNSYKIVYCFLVP------ 180

Query: 181 VVPDSRRRTGPEPDTEYCEQLDATVPVPPMGLSLLT 214
            VP    ++   PD+EYCEQLDAT+P  P  LSLL+
Sbjct: 181 -VPAGNGKS--HPDSEYCEQLDATLP-GPWDLSLLS 199

[Close]

BLAST of Spo28094.1 vs. ExPASy Swiss-Prot
Match: ASP_THECC (21 kDa seed protein OS=Theobroma cacao GN=ASP PE=2 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 7.800e-17
Identity = 63/170 (37.06%), Postives = 91/170 (53.53%), Query Frame = 1


		  
Query: 10  ATLLLTLLFLSPP----TATAKDSVVLDVDGNPVEAGSQYYVLTSGWGGAGRGGLTAASL 69
           A +LL   F S       A A +S VLD DG+ ++ G QYYVL+S  G  G G     + 
Sbjct: 6   AVVLLLFAFTSKSYFFGVANAANSPVLDTDGDELQTGVQYYVLSSISGAGGGGLALGRAT 65

Query: 70  GGPCPLYVGQYRLWVQKGSPVTFYPSNPSQKHITQSSDVNIAFAP--NPICRNQGVWQL- 129
           G  CP  V Q R  +  G+PV F  ++     +  S+DVNI F P  + +C    VW+L 
Sbjct: 66  GQSCPEIVVQRRSDLDNGTPVIFSNADSKDDVVRVSTDVNIEFVPIRDRLCSTSTVWRLD 125

Query: 130 GYNGIS--FYITTNGVIGKQEDVDTFANWFKIEQAGGLPGAYKIVYCFAV 171
            Y+  +  +++TT+GV G +   +T  +WFKIE+AG L   YK  +C +V
Sbjct: 126 NYDNSAGKWWVTTDGVKG-EPGPNTLCSWFKIEKAGVL--GYKFRFCPSV 172

[Close]

BLAST of Spo28094.1 vs. ExPASy Swiss-Prot
Match: MIRA_SYNDU (Miraculin OS=Synsepalum dulcificum PE=1 SV=3)

HSP 1 Score: 75.5 bits (184), Expect = 8.900e-13
Identity = 53/156 (33.97%), Postives = 80/156 (51.28%), Query Frame = 1


		  
Query: 22  PTATAKDSV---VLDVDGNPVEAGSQYYVLTSGWGGAGRGGLTAASLGGP--CPLYVGQY 81
           P  +A DS    VLD+DG  +  G+ YY++       G   ++A +  G   CP  V Q 
Sbjct: 24  PLLSAADSAPNPVLDIDGEKLRTGTNYYIVPVLRDHGGGLTVSATTPNGTFVCPPRVVQT 83

Query: 82  RLWVQKGSPVTFYPSNPSQKHITQSSDVNIAFAPNPICR--NQGVWQLGYNGIS---FYI 141
           R  V    P+ F+P NP +  +  S+D+NI F+    CR  +  VW+L     S   +++
Sbjct: 84  RKEVDHDRPLAFFPENPKEDVVRVSTDLNINFSAFMPCRWTSSTVWRLDKYDESTGQYFV 143

Query: 142 TTNGVIGKQEDVDTFANWFKIEQAGGLPGAYKIVYC 168
           T  GV G     +T ++WFKIE+  G  G YK+V+C
Sbjct: 144 TIGGVKG-NPGPETISSWFKIEEFCG-SGFYKLVFC 177

[Close]

BLAST of Spo28094.1 vs. ExPASy Swiss-Prot
Match: DRTI_DELRE (Kunitz-type serine protease inhibitor DrTI OS=Delonix regia PE=1 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 1.100e-10
Identity = 44/140 (31.43%), Postives = 67/140 (47.86%), Query Frame = 1


		  
Query: 31  VLDVDGNPVEAGSQYYVLTSGWGGAGRGGLTAASLGGPCPLYVGQYRLWVQKGSPVTFYP 90
           V D++G PV  GS+YY++++  G  G G     + G  CP+ + Q +  +Q G PV F  
Sbjct: 6   VYDIEGYPVFLGSEYYIVSAIIGAGGGGVRPGRTRGSMCPMSIIQEQSDLQMGLPVRFSS 65

Query: 91  SNPSQKHITQSSDVNIAFAPNPICRNQGVWQLGYNGISFYITTNGVIGKQED---VDTFA 150
              SQ  I   +++ I F   P C     W +  +     +     IG  ED    +   
Sbjct: 66  PEESQGKIYTDTELEIEFVEKPDCAESSKWVIVKDSGEARV----AIGGSEDHPQGELVR 125

Query: 151 NWFKIEQAGGLPGAYKIVYC 168
            +FKIE+ G L  AYK+V+C
Sbjct: 126 GFFKIEKLGSL--AYKLVFC 139

[Close]

BLAST of Spo28094.1 vs. ExPASy Swiss-Prot
Match: LSPI_CARPA (Latex serine proteinase inhibitor OS=Carica papaya PE=1 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 9.200e-10
Identity = 47/147 (31.97%), Postives = 72/147 (48.98%), Query Frame = 1


		  
Query: 31  VLDVDGNPVEAGSQYYVLTSGWGGAGRGGLTAASLGG--PCPLYVGQYRLWVQKGSPVTF 90
           ++D+DG PV  G  Y+V+++ W GAG GGLT    G    CPL V Q       G P+ F
Sbjct: 6   IVDIDGKPVLYGVDYFVVSAIW-GAGGGGLTVYGPGNKKKCPLSVVQDPF--DNGEPIIF 65

Query: 91  YP-SNPSQKHITQSSDVNIAFAPNPICRNQGVWQLG-YNGISFYITTNGVIGKQEDVDTF 150
               N     + +S D+N+ F     C     W++  + G+  +  T G        ++ 
Sbjct: 66  SAIKNVKDNIVFESVDLNVKFNITINCNETTAWKVDRFPGVIGWTVTLGGEKGYHGFEST 125

Query: 151 ANWFKIEQAGGLPGAYKIVYCFAVPST 174
            + FKI++A GLP +YK  +C + P T
Sbjct: 126 HSMFKIKKA-GLPFSYKFHFCPSYPRT 148

[Close]

BLAST of Spo28094.1 vs. ExPASy Swiss-Prot
Match: SPORB_IPOBA (Sporamin B OS=Ipomoea batatas GN=GSPO-B1 PE=2 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 2.700e-9
Identity = 52/156 (33.33%), Postives = 71/156 (45.51%), Query Frame = 1


		  
Query: 19  LSPPTATAK-DSVVLDVDGNPVEAGSQYYVLTSGWGGAGRGGLTAASL---GGPCPLYVG 78
           L P   TA  ++ VLD++G+ V AG  YY++++ W GAG GGL    L      C   V 
Sbjct: 28  LRPAHETASSETPVLDINGDEVRAGENYYIVSAIW-GAGGGGLRLVRLDSSSNECASDVI 87

Query: 79  QYRLWVQKGSPVTFYPSNPSQKHITQSSDVNIAF--APNPICRNQGVWQLGYNGIS-FYI 138
             R     G P+T  P++P    +  S+     F  A N +C N   W + ++  S  Y 
Sbjct: 88  VSRSDFDNGDPITITPADPEATVVMPSTYQTFRFNIATNKLCVNNVNWGIKHDSESGQYF 147

Query: 139 TTNGVIGKQEDVDTFANWFKIEQAGGLPGAYKIVYC 168
              G     E V   +N FKIE       AYKI YC
Sbjct: 148 VKAG-----EFVSDNSNQFKIEVVNDNLNAYKISYC 177

[Close]

BLAST of Spo28094.1 vs. TAIR (Arabidopsis)
Match: AT1G73325.1 (Kunitz family trypsin and protease inhibitor protein)

HSP 1 Score: 59.7 bits (143), Expect = 2.900e-9
Identity = 49/162 (30.25%), Postives = 80/162 (49.38%), Query Frame = 1


		  
Query: 13  LLTLLFLSPPTATAKDS-VVLDVDGNPVEAGSQYYVLTS--GWGGAGRGGLTAASLGGPC 72
           +L+ +F +   A A  S VVLD+ G+PV++  QYY++ +  G GG         S    C
Sbjct: 13  VLSAIFTAASAADATPSQVVLDIAGHPVQSNVQYYIIPAKIGTGGGLIPSNRNLSTQDLC 72

Query: 73  -PLYVGQYRLWVQKGSPVTFYPSNPSQKHITQSSDVNIAFAPNP-ICRNQGVWQLGYNG- 132
             L + Q       G PVTF P N   KH+  S+ +N+ F     +C +  VW++ ++  
Sbjct: 73  LNLDIVQSSSPFVSGLPVTFSPLNTKVKHVQLSASLNLEFDSTVWLCPDSKVWRIDHSVQ 132

Query: 133 -ISFYITTNGVIGKQEDVDTFANWFKIEQAGGLPGAYKIVYC 168
               +++  G  GK        +WF+I++ G    AYK++YC
Sbjct: 133 LRKSFVSIGGQKGKGN------SWFQIQEDG---DAYKLMYC 165

The following BLAST results are available for this feature:

BLAST of Spo28094.1 vs. NCBI nr
BLAST of Spo28094.1 vs. UniProtKB/TrEMBL
BLAST of Spo28094.1 vs. ExPASy Swiss-Prot
BLAST of Spo28094.1 vs. TAIR (Arabidopsis)

BLAST of Spo28094.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902228865\|gb\|KNA21296.1\|	2.6e-137	100.	hypothetical protein SOVF_0445...	[more]
gi\|902188089\|gb\|KNA11068.1\|	3.4e-89	71.9	hypothetical protein SOVF_1385...	[more]
gi\|902228866\|gb\|KNA21297.1\|	7.2e-79	68.9	hypothetical protein SOVF_0445...	[more]
gi\|902188088\|gb\|KNA11067.1\|	1.8e-77	68.6	hypothetical protein SOVF_1385...	[more]
gi\|902078036\|gb\|KNA03096.1\|	4.4e-60	62.5	hypothetical protein SOVF_2124...	[more]

BLAST of Spo28094.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9RP34_SPIOL	1.8e-137	100.	Uncharacterized protein OS=Spi...	[more]
A0A0K9QWG7_SPIOL	2.4e-89	71.9	Uncharacterized protein OS=Spi...	[more]
A0A0K9RR37_SPIOL	5.0e-79	68.9	Uncharacterized protein OS=Spi...	[more]
A0A0K9QUV4_SPIOL	1.2e-77	68.6	Uncharacterized protein OS=Spi...	[more]
A0A0K9Q9A5_SPIOL	3.0e-60	62.5	Uncharacterized protein OS=Spi...	[more]

BLAST of Spo28094.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
ASP_THECC	7.8e-17	37.0	21 kDa seed protein OS=Theobro...	[more]
MIRA_SYNDU	8.9e-13	33.9	Miraculin OS=Synsepalum dulcif...	[more]
DRTI_DELRE	1.1e-10	31.4	Kunitz-type serine protease in...	[more]
LSPI_CARPA	9.2e-10	31.9	Latex serine proteinase inhibi...	[more]
SPORB_IPOBA	2.7e-9	33.3	Sporamin B OS=Ipomoea batatas ...	[more]

BLAST of Spo28094.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 1


Match Name	E-value	Identity	Description
AT1G73325.1	2.9e-9	30.2	Kunitz family trypsin and prot...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002160	Proteinase inhibitor I3, Kunitz legume	PRINTS	PR00291	KUNITZINHBTR	coord: 69..89 score: 5.1E-12coord: 149..168 score: 5.1E-12coord: 30..59 score: 5.1
IPR002160	Proteinase inhibitor I3, Kunitz legume	PFAM	PF00197	Kunitz_legume	coord: 31..169 score: 1.0
IPR002160	Proteinase inhibitor I3, Kunitz legume	SMART	SM00452	kul_2	coord: 30..232 score: 4.8
IPR002160	Proteinase inhibitor I3, Kunitz legume	PROSITE	PS00283	SOYBEAN_KUNITZ	coord: 31..47 scor
IPR011065	Kunitz inhibitor ST1-like	unknown	SSF50386	STI-like	coord: 28..170 score: 9.53
None	No IPR available	GENE3D	2.80.10.50		coord: 27..170 score: 7.2
None	No IPR available	PANTHER	PTHR33107	FAMILY NOT NAMED	coord: 8..167 score: 4.0

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009405	pathogenesis
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0004866	endopeptidase inhibitor activity

RNA-Seq Expression

Co-expression


Gene	r value	Expression
Spo17442	0.73	Barchart \| Table
Spo28095	0.70	Barchart \| Table
Spo17427	0.67	Barchart \| Table