Spo28095 (gene) | SpinachBase

Overview


Name	Spo28095
Type	gene
Organism	Spinacia oleracea (Spinach)
Description	Kunitz trypsin inhibitor
Location	chr1 : 47381325 .. 47382077 (-)

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTAATCACCTTCTTCTTCCAGCTGCAACCATTTTCCTAACCCTCCTTTTTCTCTCTCCTGCCACGGCCACGGCCACGGCCGCCGACTCCGTAGTCGTTGACATCGATGGTAACCCATTAGAAGCAGGCTCACAATACTACGTCCTCACTTCGGGATCCAGGGGAGCAGGCCGTGGCGGCTTAAACGCTGTACCCTTGGGTGGCATTTGCCCTTTATACGTAGGTCAACATAGGAGCTGGAATAATCGAGGCAGCCCCGTAAGTTTCTACCCTGCCGACCTTTCACAGAAAAACATCACACTAAGTTCAGACGTCAACATTGCTTTCGGGCTAAGCGCCATGTGCAGGAACCAAGGGGTGTGGCAGCTAAGTTCAAATGAGCTTGGGGTATTCTACATAACTACCAATGGAGAAATCGGAAACCCCGGTGCTGAAACCTTCACCAACTGGTTCAAGATCGAGAAACTACCGTTTCCTAAATCTAATTCATATAGGATAGTGTATTGTTTCGCAATACCTAAACCTGGTCCTGAACCTGTTGTTCCTGATAGTCGTCGTACTGGTCCTGAATCTGTTGTTCCTACTGAGTATTGTCAACAGTTGGATGCTACTCTACCCGGCCCTTGGGATTTGTCGATGTTAAGTCTTGTACCAATTGATAACCCTCTTCCCTTCTTTGGTTTTGTATTCAAGAAGGTCACTACTACTACTACTACTGCTAGTACTACTACTGCCAGTAGTAGCTACTAG

mRNA sequence

ATGGCTAATCACCTTCTTCTTCCAGCTGCAACCATTTTCCTAACCCTCCTTTTTCTCTCTCCTGCCACGGCCACGGCCACGGCCGCCGACTCCGTAGTCGTTGACATCGATGGTAACCCATTAGAAGCAGGCTCACAATACTACGTCCTCACTTCGGGATCCAGGGGAGCAGGCCGTGGCGGCTTAAACGCTGTACCCTTGGGTGGCATTTGCCCTTTATACGTAGGTCAACATAGGAGCTGGAATAATCGAGGCAGCCCCGTAAGTTTCTACCCTGCCGACCTTTCACAGAAAAACATCACACTAAGTTCAGACGTCAACATTGCTTTCGGGCTAAGCGCCATGTGCAGGAACCAAGGGGTGTGGCAGCTAAGTTCAAATGAGCTTGGGGTATTCTACATAACTACCAATGGAGAAATCGGAAACCCCGGTGCTGAAACCTTCACCAACTGGTTCAAGATCGAGAAACTACCGTTTCCTAAATCTAATTCATATAGGATAGTGTATTGTTTCGCAATACCTAAACCTGGTCCTGAACCTGTTGTTCCTGATAGTCGTCGTACTGGTCCTGAATCTGTTGTTCCTACTGAGTATTGTCAACAGTTGGATGCTACTCTACCCGGCCCTTGGGATTTGTCGATGTTAAGTCTTGTACCAATTGATAACCCTCTTCCCTTCTTTGGTTTTGTATTCAAGAAGGTCACTACTACTACTACTACTGCTAGTACTACTACTGCCAGTAGTAGCTACTAG

Coding sequence (CDS)

ATGGCTAATCACCTTCTTCTTCCAGCTGCAACCATTTTCCTAACCCTCCTTTTTCTCTCTCCTGCCACGGCCACGGCCACGGCCGCCGACTCCGTAGTCGTTGACATCGATGGTAACCCATTAGAAGCAGGCTCACAATACTACGTCCTCACTTCGGGATCCAGGGGAGCAGGCCGTGGCGGCTTAAACGCTGTACCCTTGGGTGGCATTTGCCCTTTATACGTAGGTCAACATAGGAGCTGGAATAATCGAGGCAGCCCCGTAAGTTTCTACCCTGCCGACCTTTCACAGAAAAACATCACACTAAGTTCAGACGTCAACATTGCTTTCGGGCTAAGCGCCATGTGCAGGAACCAAGGGGTGTGGCAGCTAAGTTCAAATGAGCTTGGGGTATTCTACATAACTACCAATGGAGAAATCGGAAACCCCGGTGCTGAAACCTTCACCAACTGGTTCAAGATCGAGAAACTACCGTTTCCTAAATCTAATTCATATAGGATAGTGTATTGTTTCGCAATACCTAAACCTGGTCCTGAACCTGTTGTTCCTGATAGTCGTCGTACTGGTCCTGAATCTGTTGTTCCTACTGAGTATTGTCAACAGTTGGATGCTACTCTACCCGGCCCTTGGGATTTGTCGATGTTAAGTCTTGTACCAATTGATAACCCTCTTCCCTTCTTTGGTTTTGTATTCAAGAAGGTCACTACTACTACTACTACTGCTAGTACTACTACTGCCAGTAGTAGCTACTAG

Protein sequence

MANHLLLPAATIFLTLLFLSPATATATAADSVVVDIDGNPLEAGSQYYVLTSGSRGAGRGGLNAVPLGGICPLYVGQHRSWNNRGSPVSFYPADLSQKNITLSSDVNIAFGLSAMCRNQGVWQLSSNELGVFYITTNGEIGNPGAETFTNWFKIEKLPFPKSNSYRIVYCFAIPKPGPEPVVPDSRRTGPESVVPTEYCQQLDATLPGPWDLSMLSLVPIDNPLPFFGFVFKKVTTTTTTASTTTASSSY

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spo28095.1	Spo28095.1	mRNA

Homology

[Close]

BLAST of Spo28095.1 vs. NCBI nr
Match: gi|902228866|gb|KNA21297.1| (hypothetical protein SOVF_044560 [Spinacia oleracea])

HSP 1 Score: 497.7 bits (1280), Expect = 1.200e-137
Identity = 249/252 (98.81%), Postives = 250/252 (99.21%), Query Frame = 1


		  
Query: 1   MANHLLLPAATIFLTLLFLSPATATATAADSVVVDIDGNPLEAGSQYYVLTSGSRGAGRG 60
           MANHLLLPAATIFLTLLFLSPATATATAADSVVVDIDGNPLEAGSQYYVLTSGSRGAGRG
Sbjct: 1   MANHLLLPAATIFLTLLFLSPATATATAADSVVVDIDGNPLEAGSQYYVLTSGSRGAGRG 60

Query: 61  GLNAVPLGGICPLYVGQHRSWNNRGSPVSFYPADLSQKNITLSSDVNIAFGLSAMCRNQG 120
           GLNAVPLGGICPLYVGQHRSWNNRGSPVSFYPADLSQKNITLSSDVNIAFGLSAMCRNQG
Sbjct: 61  GLNAVPLGGICPLYVGQHRSWNNRGSPVSFYPADLSQKNITLSSDVNIAFGLSAMCRNQG 120

Query: 121 VWQLSSNELGVFYITTNGEIGNPGAETFTNWFKIEKLPFPKSNSYRIVYCFAIPKPGPEP 180
           VWQLSSNELGVFYITTNGEIGNPGAETFTNWFKIEKLPFPKSNSYRIVYCFA+PKPGPEP
Sbjct: 121 VWQLSSNELGVFYITTNGEIGNPGAETFTNWFKIEKLPFPKSNSYRIVYCFAVPKPGPEP 180

Query: 181 --VVPDSRRTGPESVVPTEYCQQLDATLPGPWDLSMLSLVPIDNPLPFFGFVFKKVTTTT 240
             VVPDSRRTGPESVVPTEYCQQLDATLPGPWDLSMLSLVPIDNPLPFFGFVFKKVTTTT
Sbjct: 181 VHVVPDSRRTGPESVVPTEYCQQLDATLPGPWDLSMLSLVPIDNPLPFFGFVFKKVTTTT 240

Query: 241 TTASTTTASSSY 251
           TTASTTTASSSY
Sbjct: 241 TTASTTTASSSY 252

[Close]

BLAST of Spo28095.1 vs. NCBI nr
Match: gi|902188089|gb|KNA11068.1| (hypothetical protein SOVF_138590 [Spinacia oleracea])

HSP 1 Score: 349.0 bits (894), Expect = 7.000e-93
Identity = 181/245 (73.88%), Postives = 202/245 (82.45%), Query Frame = 1


		  
Query: 1   MANHLLLPAATIFLTLLFLSPATATATAADSVVVDIDGNPLEAGSQYYVLTSGSRGAGRG 60
           MANHLLLPAATI LTLLFLSP T TA  ADSVV+D+DGNPLEAGSQYYVLT G+RGAGRG
Sbjct: 1   MANHLLLPAATIILTLLFLSPPTVTA--ADSVVLDMDGNPLEAGSQYYVLTLGTRGAGRG 60

Query: 61  GLNAVPLGGICPLYVGQHRSWNNRGSPVSFYPADLSQKNITLSSDVNIAFGLSAMCRNQG 120
           GL A  L G CPLYVGQHRSW  +GSPV+FYPAD SQKNIT SSDVNIAF  S +CRNQG
Sbjct: 61  GLTAERLTGPCPLYVGQHRSWTQQGSPVTFYPADSSQKNITQSSDVNIAFNPSPICRNQG 120

Query: 121 VWQLSSNELGVFYITTNGEIGNPGAETFTNWFKIEKLPFPKSNSYRIVYCFAIPKPGPEP 180
           VWQLSSN LGVF++TTNG IGNPGAETFTNWFKIEK    + +SY+IVYCF +P PGP P
Sbjct: 121 VWQLSSNGLGVFHVTTNGVIGNPGAETFTNWFKIEKAS-SEPDSYKIVYCFVVPGPGPVP 180

Query: 181 VVPDSRRTGPESVVPTEYCQQLDATLP-GPWDLSMLSLVPIDNPLPFFGFVFKKVTTTTT 240
             P++ +  P+     EYC+QLDAT+P  P D S+LSLVPIDNPLPFFGFVFKKVTTTTT
Sbjct: 181 APPETGKNRPD-----EYCEQLDATIPVPPLDFSLLSLVPIDNPLPFFGFVFKKVTTTTT 237

Query: 241 TASTT 245
           T S++
Sbjct: 241 TISSS 237

[Close]

BLAST of Spo28095.1 vs. NCBI nr
Match: gi|902188088|gb|KNA11067.1| (hypothetical protein SOVF_138580 [Spinacia oleracea])

HSP 1 Score: 315.5 bits (807), Expect = 8.500e-83
Identity = 164/237 (69.20%), Postives = 185/237 (78.06%), Query Frame = 1


		  
Query: 1   MANHLLLPAATIFLTLLFLSPATATATAADSVVVDIDGNPLEAGSQYYVLTSGSRGAGRG 60
           MA+HLLL AATI LTL FLSP TA    ADSVV+DIDGNP+EAGSQYYVLT G+RGAGRG
Sbjct: 1   MAHHLLLAAATILLTLFFLSPPTA----ADSVVLDIDGNPVEAGSQYYVLTRGTRGAGRG 60

Query: 61  GLNAVPLGGICPLYVGQHRSWNNRGSPVSFYPADLSQKNITLSSDVNIAFGLSAMCRNQG 120
           GL A PLGG+CPLYVGQHRSW  +GSPV+F+PA+ SQK IT SSDVNIAFGL+ +CRN G
Sbjct: 61  GLTAAPLGGLCPLYVGQHRSWTQQGSPVTFFPANPSQKYITQSSDVNIAFGLNPICRNLG 120

Query: 121 VWQLSSNELGVFYITTNGEIGNPGAETFTNWFKIEKLPFPKSNSYRIVYCFAIPKPGPEP 180
           VWQL  +  GV Y+TTNG IGNPGAETFTNWFKIE    P+  SY+IVYCF +P P    
Sbjct: 121 VWQLGYSFSGVPYVTTNGVIGNPGAETFTNWFKIEHAFGPE--SYKIVYCFLVPAP---- 180

Query: 181 VVPDSRRTGPESVVPTEYCQQLDATLPGPWDLSMLSLVPIDNPLPFFGFVFKKVTTT 238
                       + P EYC+QLDAT+PGP DL+MLSLVPID PLPFFGFVFKKVT+T
Sbjct: 181 --------WYSRMSPYEYCEQLDATIPGPSDLAMLSLVPIDKPLPFFGFVFKKVTST 219

[Close]

BLAST of Spo28095.1 vs. NCBI nr
Match: gi|902228865|gb|KNA21296.1| (hypothetical protein SOVF_044550 [Spinacia oleracea])

HSP 1 Score: 307.4 bits (786), Expect = 2.300e-80
Identity = 168/243 (69.14%), Postives = 191/243 (78.60%), Query Frame = 1


		  
Query: 1   MANHLLLPAATIFLTLLFLSPATATATAADSVVVDIDGNPLEAGSQYYVLTSGSRGAGRG 60
           MA+HLLLPAAT+ LTLLFLSP TATA   DSVV+D+DGNP+EAGSQYYVLTSG  GAGRG
Sbjct: 1   MAHHLLLPAATLLLTLLFLSPPTATAK--DSVVLDVDGNPVEAGSQYYVLTSGWGGAGRG 60

Query: 61  GLNAVPLGGICPLYVGQHRSWNNRGSPVSFYPADLSQKNITLSSDVNIAFGLSAMCRNQG 120
           GL A  LGG CPLYVGQ+R W  +GSPV+FYP++ SQK+IT SSDVNIAF  + +CRNQG
Sbjct: 61  GLTAASLGGPCPLYVGQYRLWVQKGSPVTFYPSNPSQKHITQSSDVNIAFAPNPICRNQG 120

Query: 121 VWQLSSNELGVFYITTNGEIG-NPGAETFTNWFKIEK---LPFPKSNSYRIVYCFAIPKP 180
           VWQL  N +  FYITTNG IG     +TF NWFKIE+   LP     +Y+IVYCFA+P  
Sbjct: 121 VWQLGYNGIS-FYITTNGVIGKQEDVDTFANWFKIEQAGGLP----GAYKIVYCFAVPST 180

Query: 181 GPEPVVPDS-RRTGPESVVPTEYCQQLDATLP-GPWDLSMLSLVPIDNPLPFFGFVFKKV 238
           GPEPVVPDS RRTGPE    TEYC+QLDAT+P  P  LS+L+ VPIDNPLPFFGFVFKKV
Sbjct: 181 GPEPVVPDSRRRTGPEP--DTEYCEQLDATVPVPPMGLSLLTPVPIDNPLPFFGFVFKKV 234

[Close]

BLAST of Spo28095.1 vs. NCBI nr
Match: gi|902078036|gb|KNA03096.1| (hypothetical protein SOVF_212410 [Spinacia oleracea])

HSP 1 Score: 301.6 bits (771), Expect = 1.300e-78
Identity = 154/217 (70.97%), Postives = 176/217 (81.11%), Query Frame = 1


		  
Query: 1   MANHLLLPAATIFLTLLFLSPATATATAADSVVVDIDGNPLEAGSQYYVLTSGSRGAGRG 60
           MANHLLL AATI    LFLSP T TA  ADSVV+D+DGNP+EAGS+YYV T GSRGA RG
Sbjct: 1   MANHLLLLAATI----LFLSPPTVTA--ADSVVLDMDGNPVEAGSRYYVQTLGSRGASRG 60

Query: 61  GLNAVPLGGICPLYVGQHRSWNNRGSPVSFYPADLSQKNITLSSDVNIAFGLSAMCRNQG 120
           GL A PL GICPLYVGQHRSW N+G P++FYPAD SQKNITLSSDVNIAFGL+ +CRNQG
Sbjct: 61  GLAAEPLSGICPLYVGQHRSWTNQGRPITFYPADPSQKNITLSSDVNIAFGLNGLCRNQG 120

Query: 121 VWQLSSNELGVFYITTNGEIGNPGAETFTNWFKIEKLPFPKSNSYRIVYCFAIPKPGPEP 180
           VWQL+SN  GVFY+TTNGEIGNPGAETFTNWFK+E+   P +NSY+IVYCF +P P    
Sbjct: 121 VWQLTSNGDGVFYVTTNGEIGNPGAETFTNWFKLEE-ALPGTNSYKIVYCFLVPVPA--- 180

Query: 181 VVPDSRRTGPESVVPTEYCQQLDATLPGPWDLSMLSL 218
               + ++ P+S    EYC+QLDATLPGPWDLS+LSL
Sbjct: 181 ---GNGKSHPDS----EYCEQLDATLPGPWDLSLLSL 200

[Close]

BLAST of Spo28095.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RR37_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_044560 PE=4 SV=1)

HSP 1 Score: 497.7 bits (1280), Expect = 8.500e-138
Identity = 249/252 (98.81%), Postives = 250/252 (99.21%), Query Frame = 1


		  
Query: 1   MANHLLLPAATIFLTLLFLSPATATATAADSVVVDIDGNPLEAGSQYYVLTSGSRGAGRG 60
           MANHLLLPAATIFLTLLFLSPATATATAADSVVVDIDGNPLEAGSQYYVLTSGSRGAGRG
Sbjct: 1   MANHLLLPAATIFLTLLFLSPATATATAADSVVVDIDGNPLEAGSQYYVLTSGSRGAGRG 60

Query: 61  GLNAVPLGGICPLYVGQHRSWNNRGSPVSFYPADLSQKNITLSSDVNIAFGLSAMCRNQG 120
           GLNAVPLGGICPLYVGQHRSWNNRGSPVSFYPADLSQKNITLSSDVNIAFGLSAMCRNQG
Sbjct: 61  GLNAVPLGGICPLYVGQHRSWNNRGSPVSFYPADLSQKNITLSSDVNIAFGLSAMCRNQG 120

Query: 121 VWQLSSNELGVFYITTNGEIGNPGAETFTNWFKIEKLPFPKSNSYRIVYCFAIPKPGPEP 180
           VWQLSSNELGVFYITTNGEIGNPGAETFTNWFKIEKLPFPKSNSYRIVYCFA+PKPGPEP
Sbjct: 121 VWQLSSNELGVFYITTNGEIGNPGAETFTNWFKIEKLPFPKSNSYRIVYCFAVPKPGPEP 180

Query: 181 --VVPDSRRTGPESVVPTEYCQQLDATLPGPWDLSMLSLVPIDNPLPFFGFVFKKVTTTT 240
             VVPDSRRTGPESVVPTEYCQQLDATLPGPWDLSMLSLVPIDNPLPFFGFVFKKVTTTT
Sbjct: 181 VHVVPDSRRTGPESVVPTEYCQQLDATLPGPWDLSMLSLVPIDNPLPFFGFVFKKVTTTT 240

Query: 241 TTASTTTASSSY 251
           TTASTTTASSSY
Sbjct: 241 TTASTTTASSSY 252

[Close]

BLAST of Spo28095.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QWG7_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_138590 PE=4 SV=1)

HSP 1 Score: 349.0 bits (894), Expect = 4.900e-93
Identity = 181/245 (73.88%), Postives = 202/245 (82.45%), Query Frame = 1


		  
Query: 1   MANHLLLPAATIFLTLLFLSPATATATAADSVVVDIDGNPLEAGSQYYVLTSGSRGAGRG 60
           MANHLLLPAATI LTLLFLSP T TA  ADSVV+D+DGNPLEAGSQYYVLT G+RGAGRG
Sbjct: 1   MANHLLLPAATIILTLLFLSPPTVTA--ADSVVLDMDGNPLEAGSQYYVLTLGTRGAGRG 60

Query: 61  GLNAVPLGGICPLYVGQHRSWNNRGSPVSFYPADLSQKNITLSSDVNIAFGLSAMCRNQG 120
           GL A  L G CPLYVGQHRSW  +GSPV+FYPAD SQKNIT SSDVNIAF  S +CRNQG
Sbjct: 61  GLTAERLTGPCPLYVGQHRSWTQQGSPVTFYPADSSQKNITQSSDVNIAFNPSPICRNQG 120

Query: 121 VWQLSSNELGVFYITTNGEIGNPGAETFTNWFKIEKLPFPKSNSYRIVYCFAIPKPGPEP 180
           VWQLSSN LGVF++TTNG IGNPGAETFTNWFKIEK    + +SY+IVYCF +P PGP P
Sbjct: 121 VWQLSSNGLGVFHVTTNGVIGNPGAETFTNWFKIEKAS-SEPDSYKIVYCFVVPGPGPVP 180

Query: 181 VVPDSRRTGPESVVPTEYCQQLDATLP-GPWDLSMLSLVPIDNPLPFFGFVFKKVTTTTT 240
             P++ +  P+     EYC+QLDAT+P  P D S+LSLVPIDNPLPFFGFVFKKVTTTTT
Sbjct: 181 APPETGKNRPD-----EYCEQLDATIPVPPLDFSLLSLVPIDNPLPFFGFVFKKVTTTTT 237

Query: 241 TASTT 245
           T S++
Sbjct: 241 TISSS 237

[Close]

BLAST of Spo28095.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QUV4_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_138580 PE=4 SV=1)

HSP 1 Score: 315.5 bits (807), Expect = 6.000e-83
Identity = 164/237 (69.20%), Postives = 185/237 (78.06%), Query Frame = 1


		  
Query: 1   MANHLLLPAATIFLTLLFLSPATATATAADSVVVDIDGNPLEAGSQYYVLTSGSRGAGRG 60
           MA+HLLL AATI LTL FLSP TA    ADSVV+DIDGNP+EAGSQYYVLT G+RGAGRG
Sbjct: 1   MAHHLLLAAATILLTLFFLSPPTA----ADSVVLDIDGNPVEAGSQYYVLTRGTRGAGRG 60

Query: 61  GLNAVPLGGICPLYVGQHRSWNNRGSPVSFYPADLSQKNITLSSDVNIAFGLSAMCRNQG 120
           GL A PLGG+CPLYVGQHRSW  +GSPV+F+PA+ SQK IT SSDVNIAFGL+ +CRN G
Sbjct: 61  GLTAAPLGGLCPLYVGQHRSWTQQGSPVTFFPANPSQKYITQSSDVNIAFGLNPICRNLG 120

Query: 121 VWQLSSNELGVFYITTNGEIGNPGAETFTNWFKIEKLPFPKSNSYRIVYCFAIPKPGPEP 180
           VWQL  +  GV Y+TTNG IGNPGAETFTNWFKIE    P+  SY+IVYCF +P P    
Sbjct: 121 VWQLGYSFSGVPYVTTNGVIGNPGAETFTNWFKIEHAFGPE--SYKIVYCFLVPAP---- 180

Query: 181 VVPDSRRTGPESVVPTEYCQQLDATLPGPWDLSMLSLVPIDNPLPFFGFVFKKVTTT 238
                       + P EYC+QLDAT+PGP DL+MLSLVPID PLPFFGFVFKKVT+T
Sbjct: 181 --------WYSRMSPYEYCEQLDATIPGPSDLAMLSLVPIDKPLPFFGFVFKKVTST 219

[Close]

BLAST of Spo28095.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RP34_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_044550 PE=4 SV=1)

HSP 1 Score: 307.4 bits (786), Expect = 1.600e-80
Identity = 168/243 (69.14%), Postives = 191/243 (78.60%), Query Frame = 1


		  
Query: 1   MANHLLLPAATIFLTLLFLSPATATATAADSVVVDIDGNPLEAGSQYYVLTSGSRGAGRG 60
           MA+HLLLPAAT+ LTLLFLSP TATA   DSVV+D+DGNP+EAGSQYYVLTSG  GAGRG
Sbjct: 1   MAHHLLLPAATLLLTLLFLSPPTATAK--DSVVLDVDGNPVEAGSQYYVLTSGWGGAGRG 60

Query: 61  GLNAVPLGGICPLYVGQHRSWNNRGSPVSFYPADLSQKNITLSSDVNIAFGLSAMCRNQG 120
           GL A  LGG CPLYVGQ+R W  +GSPV+FYP++ SQK+IT SSDVNIAF  + +CRNQG
Sbjct: 61  GLTAASLGGPCPLYVGQYRLWVQKGSPVTFYPSNPSQKHITQSSDVNIAFAPNPICRNQG 120

Query: 121 VWQLSSNELGVFYITTNGEIG-NPGAETFTNWFKIEK---LPFPKSNSYRIVYCFAIPKP 180
           VWQL  N +  FYITTNG IG     +TF NWFKIE+   LP     +Y+IVYCFA+P  
Sbjct: 121 VWQLGYNGIS-FYITTNGVIGKQEDVDTFANWFKIEQAGGLP----GAYKIVYCFAVPST 180

Query: 181 GPEPVVPDS-RRTGPESVVPTEYCQQLDATLP-GPWDLSMLSLVPIDNPLPFFGFVFKKV 238
           GPEPVVPDS RRTGPE    TEYC+QLDAT+P  P  LS+L+ VPIDNPLPFFGFVFKKV
Sbjct: 181 GPEPVVPDSRRRTGPEP--DTEYCEQLDATVPVPPMGLSLLTPVPIDNPLPFFGFVFKKV 234

[Close]

BLAST of Spo28095.1 vs. UniProtKB/TrEMBL
Match: A0A0K9Q9A5_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_212410 PE=4 SV=1)

HSP 1 Score: 301.6 bits (771), Expect = 8.900e-79
Identity = 154/217 (70.97%), Postives = 176/217 (81.11%), Query Frame = 1


		  
Query: 1   MANHLLLPAATIFLTLLFLSPATATATAADSVVVDIDGNPLEAGSQYYVLTSGSRGAGRG 60
           MANHLLL AATI    LFLSP T TA  ADSVV+D+DGNP+EAGS+YYV T GSRGA RG
Sbjct: 1   MANHLLLLAATI----LFLSPPTVTA--ADSVVLDMDGNPVEAGSRYYVQTLGSRGASRG 60

Query: 61  GLNAVPLGGICPLYVGQHRSWNNRGSPVSFYPADLSQKNITLSSDVNIAFGLSAMCRNQG 120
           GL A PL GICPLYVGQHRSW N+G P++FYPAD SQKNITLSSDVNIAFGL+ +CRNQG
Sbjct: 61  GLAAEPLSGICPLYVGQHRSWTNQGRPITFYPADPSQKNITLSSDVNIAFGLNGLCRNQG 120

Query: 121 VWQLSSNELGVFYITTNGEIGNPGAETFTNWFKIEKLPFPKSNSYRIVYCFAIPKPGPEP 180
           VWQL+SN  GVFY+TTNGEIGNPGAETFTNWFK+E+   P +NSY+IVYCF +P P    
Sbjct: 121 VWQLTSNGDGVFYVTTNGEIGNPGAETFTNWFKLEE-ALPGTNSYKIVYCFLVPVPA--- 180

Query: 181 VVPDSRRTGPESVVPTEYCQQLDATLPGPWDLSMLSL 218
               + ++ P+S    EYC+QLDATLPGPWDLS+LSL
Sbjct: 181 ---GNGKSHPDS----EYCEQLDATLPGPWDLSLLSL 200

[Close]

BLAST of Spo28095.1 vs. ExPASy Swiss-Prot
Match: ASP_THECC (21 kDa seed protein OS=Theobroma cacao GN=ASP PE=2 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 1.500e-18
Identity = 66/159 (41.51%), Postives = 88/159 (55.35%), Query Frame = 1


		  
Query: 7   LPAATIFLTLLFLSPATA----TATAADSVVVDIDGNPLEAGSQYYVLTSGSRGAGRGGL 66
           +  AT  + LLF   + +     A AA+S V+D DG+ L+ G QYYVL+S S GAG GGL
Sbjct: 1   MKTATAVVLLLFAFTSKSYFFGVANAANSPVLDTDGDELQTGVQYYVLSSIS-GAGGGGL 60

Query: 67  N-AVPLGGICPLYVGQHRSWNNRGSPVSFYPADLSQKNITLSSDVNIAFG--LSAMCRNQ 126
                 G  CP  V Q RS  + G+PV F  AD     + +S+DVNI F      +C   
Sbjct: 61  ALGRATGQSCPEIVVQRRSDLDNGTPVIFSNADSKDDVVRVSTDVNIEFVPIRDRLCSTS 120

Query: 127 GVWQLSS--NELGVFYITTNGEIGNPGAETFTNWFKIEK 157
            VW+L +  N  G +++TT+G  G PG  T  +WFKIEK
Sbjct: 121 TVWRLDNYDNSAGKWWVTTDGVKGEPGPNTLCSWFKIEK 158

[Close]

BLAST of Spo28095.1 vs. ExPASy Swiss-Prot
Match: MIRA_SYNDU (Miraculin OS=Synsepalum dulcificum PE=1 SV=3)

HSP 1 Score: 87.8 bits (216), Expect = 1.800e-16
Identity = 57/174 (32.76%), Postives = 92/174 (52.87%), Query Frame = 1


		  
Query: 6   LLPAATIFLTLLFLSPATATATAADSV---VVDIDGNPLEAGSQYYVLTSGSRGAGRGGL 65
           +L  +  F++ L  + A    +AADS    V+DIDG  L  G+ YY++       G   +
Sbjct: 6   MLSLSFFFVSALLAAAANPLLSAADSAPNPVLDIDGEKLRTGTNYYIVPVLRDHGGGLTV 65

Query: 66  NAVPLGG--ICPLYVGQHRSWNNRGSPVSFYPADLSQKNITLSSDVNIAFGLSAMCR--N 125
           +A    G  +CP  V Q R   +   P++F+P +  +  + +S+D+NI F     CR  +
Sbjct: 66  SATTPNGTFVCPPRVVQTRKEVDHDRPLAFFPENPKEDVVRVSTDLNINFSAFMPCRWTS 125

Query: 126 QGVWQLSSNE--LGVFYITTNGEIGNPGAETFTNWFKIEKLPFPKSNSYRIVYC 171
             VW+L   +   G +++T  G  GNPG ET ++WFKIE+  F  S  Y++V+C
Sbjct: 126 STVWRLDKYDESTGQYFVTIGGVKGNPGPETISSWFKIEE--FCGSGFYKLVFC 177

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BLAST of Spo28095.1 vs. ExPASy Swiss-Prot
Match: IAAS_HORVU (Alpha-amylase/subtilisin inhibitor OS=Hordeum vulgare PE=1 SV=2)

HSP 1 Score: 68.9 bits (167), Expect = 8.700e-11
Identity = 51/153 (33.33%), Postives = 70/153 (45.75%), Query Frame = 1


		  
Query: 21  PATATATAADSVVVDIDGNPLEAGSQYYVLTSGSRGAGRGGLNAVP-LGGICPLYVGQHR 80
           PA  +  A    V D DG+ L A + YYVL S +R  G GGL   P  G  CPL+V Q  
Sbjct: 16  PAPPSRAADPPPVHDTDGHELRADANYYVL-SANRAHG-GGLTMAPGHGRHCPLFVSQDP 75

Query: 81  SWNNRGSPVSFYPADL--SQKNITLSSDVNIAFGLSAMCRNQGVWQLSSNELGVFYITTN 140
           +  + G PV   P  +  S K I LS+DV I+F     C     W + S           
Sbjct: 76  NGQHDGFPVRITPYGVAPSDKIIRLSTDVRISFRAYTTCLQSTEWHIDSELAAGRRHVIT 135

Query: 141 GEIGNPGAETFTNWFKIEKLPFPKSNSYRIVYC 171
           G + +P      N F+IEK    + + Y+++ C
Sbjct: 136 GPVKDPSPSGRENAFRIEKYSGAEVHEYKLMSC 166

[Close]

BLAST of Spo28095.1 vs. ExPASy Swiss-Prot
Match: IAAS_WHEAT (Endogenous alpha-amylase/subtilisin inhibitor OS=Triticum aestivum PE=1 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 1.900e-10
Identity = 47/141 (33.33%), Postives = 62/141 (43.97%), Query Frame = 1


		  
Query: 33  VVDIDGNPLEAGSQYYVLTSGSRGAGRGGLNAVP-LGGICPLYVGQHRSWNNRGSPVSFY 92
           V D DGN L A + YYVL +    A  GGL   P  G  CPL+V Q       G PV   
Sbjct: 5   VHDTDGNELRADANYYVLPANR--AHGGGLTMAPGHGRRCPLFVSQEADGQRDGLPVRIA 64

Query: 93  P--ADLSQKNITLSSDVNIAFGLSAMCRNQGVWQLSSNELGVFYITTNGEIGNPGAETFT 152
           P     S K I LS+DV I+F     C     W + S  +        G + +P      
Sbjct: 65  PHGGAPSDKIIRLSTDVRISFRAYTTCVQSTEWHIDSELVSGRRHVITGPVRDPSPSGRE 124

Query: 153 NWFKIEKLPFPKSNSYRIVYC 171
           N F+IEK    + + Y+++ C
Sbjct: 125 NAFRIEKYSGAEVHEYKLMAC 143

[Close]

BLAST of Spo28095.1 vs. ExPASy Swiss-Prot
Match: DRTI_DELRE (Kunitz-type serine protease inhibitor DrTI OS=Delonix regia PE=1 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 1.900e-10
Identity = 45/139 (32.37%), Postives = 68/139 (48.92%), Query Frame = 1


		  
Query: 33  VVDIDGNPLEAGSQYYVLTSGSRGAGRGGLN-AVPLGGICPLYVGQHRSWNNRGSPVSFY 92
           V DI+G P+  GS+YY++ S   GAG GG+      G +CP+ + Q +S    G PV F 
Sbjct: 6   VYDIEGYPVFLGSEYYIV-SAIIGAGGGGVRPGRTRGSMCPMSIIQEQSDLQMGLPVRFS 65

Query: 93  PADLSQKNITLSSDVNIAFGLSAMCRNQGVWQLSSNELGVFYITTNGEIGNPGAETFTNW 152
             + SQ  I   +++ I F     C     W +  +  G   +   G   +P  E    +
Sbjct: 66  SPEESQGKIYTDTELEIEFVEKPDCAESSKWVIVKDS-GEARVAIGGSEDHPQGELVRGF 125

Query: 153 FKIEKLPFPKSNSYRIVYC 171
           FKIEKL    S +Y++V+C
Sbjct: 126 FKIEKL---GSLAYKLVFC 139

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BLAST of Spo28095.1 vs. TAIR (Arabidopsis)
Match: AT1G17860.1 (Kunitz family trypsin and protease inhibitor protein)

HSP 1 Score: 76.3 bits (186), Expect = 3.100e-14
Identity = 62/163 (38.04%), Postives = 86/163 (52.76%), Query Frame = 1


		  
Query: 12  IFLTL-LFLSPATATATAADSVVVDIDGNPLEAGSQYYVLTSGSRGAGRGGLNAVPLGG- 71
           IFL L +F+S    T  AA   V DI+G  L  G  YY+L    RG G GGL    L   
Sbjct: 7   IFLLLAVFISHRGVTTEAAVEPVKDINGKSLLTGVNYYILPV-IRGRG-GGLTMSNLKTE 66

Query: 72  ICPLYVGQHRSWNNRGSPVSFYPADLSQKNITLSSDVNIAFGLSAMCRNQGVWQLSSNEL 131
            CP  V Q +   ++G PV F P D S + I +S+DVNI F  ++      +W+L++ + 
Sbjct: 67  TCPTSVIQDQFEVSQGLPVKFSPYDKS-RTIPVSTDVNIKFSPTS------IWELANFDE 126

Query: 132 GV--FYITTNGEIGNPGAETFTNWFKIEKLPFPKSNSYRIVYC 171
               ++I+T G  GNPG +T  NWFKI+K        Y+I +C
Sbjct: 127 TTKQWFISTCGVEGNPGQKTVDNWFKIDKF----EKDYKIRFC 156

[Close]

BLAST of Spo28095.1 vs. TAIR (Arabidopsis)
Match: AT1G73325.1 (Kunitz family trypsin and protease inhibitor protein)

HSP 1 Score: 61.6 bits (148), Expect = 7.800e-10
Identity = 47/167 (28.14%), Postives = 88/167 (52.69%), Query Frame = 1


		  
Query: 13  FLTLLFLSPATATATAADS----VVVDIDGNPLEAGSQYYVLTS--GSRGAGRGGLNAVP 72
           F+TL  LS     A+AAD+    VV+DI G+P+++  QYY++ +  G+ G        + 
Sbjct: 8   FITLTVLSAIFTAASAADATPSQVVLDIAGHPVQSNVQYYIIPAKIGTGGGLIPSNRNLS 67

Query: 73  LGGIC-PLYVGQHRSWNNRGSPVSFYPADLSQKNITLSSDVNIAFGLSA-MCRNQGVWQL 132
              +C  L + Q  S    G PV+F P +   K++ LS+ +N+ F  +  +C +  VW++
Sbjct: 68  TQDLCLNLDIVQSSSPFVSGLPVTFSPLNTKVKHVQLSASLNLEFDSTVWLCPDSKVWRI 127

Query: 133 S-SNELGVFYITTNGEIGNPGAETFTNWFKIEKLPFPKSNSYRIVYC 171
             S +L   +++  G+ G        +WF+I++      ++Y+++YC
Sbjct: 128 DHSVQLRKSFVSIGGQKGKG-----NSWFQIQE----DGDAYKLMYC 165

The following BLAST results are available for this feature:

BLAST of Spo28095.1 vs. NCBI nr
BLAST of Spo28095.1 vs. UniProtKB/TrEMBL
BLAST of Spo28095.1 vs. ExPASy Swiss-Prot
BLAST of Spo28095.1 vs. TAIR (Arabidopsis)

BLAST of Spo28095.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902228866\|gb\|KNA21297.1\|	1.2e-137	98.8	hypothetical protein SOVF_0445...	[more]
gi\|902188089\|gb\|KNA11068.1\|	7.0e-93	73.8	hypothetical protein SOVF_1385...	[more]
gi\|902188088\|gb\|KNA11067.1\|	8.5e-83	69.2	hypothetical protein SOVF_1385...	[more]
gi\|902228865\|gb\|KNA21296.1\|	2.3e-80	69.1	hypothetical protein SOVF_0445...	[more]
gi\|902078036\|gb\|KNA03096.1\|	1.3e-78	70.9	hypothetical protein SOVF_2124...	[more]

BLAST of Spo28095.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9RR37_SPIOL	8.5e-138	98.8	Uncharacterized protein OS=Spi...	[more]
A0A0K9QWG7_SPIOL	4.9e-93	73.8	Uncharacterized protein OS=Spi...	[more]
A0A0K9QUV4_SPIOL	6.0e-83	69.2	Uncharacterized protein OS=Spi...	[more]
A0A0K9RP34_SPIOL	1.6e-80	69.1	Uncharacterized protein OS=Spi...	[more]
A0A0K9Q9A5_SPIOL	8.9e-79	70.9	Uncharacterized protein OS=Spi...	[more]

BLAST of Spo28095.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
ASP_THECC	1.5e-18	41.5	21 kDa seed protein OS=Theobro...	[more]
MIRA_SYNDU	1.8e-16	32.7	Miraculin OS=Synsepalum dulcif...	[more]
IAAS_HORVU	8.7e-11	33.3	Alpha-amylase/subtilisin inhib...	[more]
IAAS_WHEAT	1.9e-10	33.3	Endogenous alpha-amylase/subti...	[more]
DRTI_DELRE	1.9e-10	32.3	Kunitz-type serine protease in...	[more]

BLAST of Spo28095.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 2


Match Name	E-value	Identity	Description
AT1G17860.1	3.1e-14	38.0	Kunitz family trypsin and prot...	[more]
AT1G73325.1	7.8e-10	28.1	Kunitz family trypsin and prot...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002160	Proteinase inhibitor I3, Kunitz legume	PRINTS	PR00291	KUNITZINHBTR	coord: 71..91 score: 8.0E-8coord: 32..61 score: 8.0E-8coord: 151..170 score: 8.
IPR002160	Proteinase inhibitor I3, Kunitz legume	PFAM	PF00197	Kunitz_legume	coord: 33..175 score: 1.7
IPR002160	Proteinase inhibitor I3, Kunitz legume	SMART	SM00452	kul_2	coord: 32..235 score: 5.1
IPR002160	Proteinase inhibitor I3, Kunitz legume	PROSITE	PS00283	SOYBEAN_KUNITZ	coord: 33..49 scor
IPR011065	Kunitz inhibitor ST1-like	unknown	SSF50386	STI-like	coord: 29..172 score: 2.99
None	No IPR available	GENE3D	2.80.10.50		coord: 29..172 score: 1.0
None	No IPR available	PANTHER	PTHR33107	FAMILY NOT NAMED	coord: 8..170 score: 6.5

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0017148	negative regulation of translation
biological_process	GO:0042254	ribosome biogenesis
biological_process	GO:0010951	negative regulation of endopeptidase activity
biological_process	GO:0009405	pathogenesis
cellular_component	GO:0005840	ribosome
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0004866	endopeptidase inhibitor activity
molecular_function	GO:0030598	rRNA N-glycosylase activity
molecular_function	GO:0003735	structural constituent of ribosome
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity

RNA-Seq Expression

Co-expression


Gene	r value	Expression
Spo17427	0.78	Barchart \| Table
Spo28328	0.74	Barchart \| Table
Spo28094	0.70	Barchart \| Table
Spo28275	0.69	Barchart \| Table
Spo17442	0.68	Barchart \| Table
Spo27275	0.67	Barchart \| Table
Spo21696	0.66	Barchart \| Table