Spo28200 (gene) | SpinachBase

Overview


Name	Spo28200
Type	gene
Organism	Spinacia oleracea (Spinach)
Description	17.6 kDa class I heat shock protein
Location	chr3 : 62875580 .. 62876107 (+)

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTCAATCCGATTCATTTTCGAGATCGATCTCGAAGGAGAATGCAATCTATGATGCCATATCATCATGGCTTCTTTGGGCGACCTGTTGAAATGTTCGACCCATTCTCCCTCTACTTTCCGAATGATTTTCCCTTCGATTTCCCAGCCTTTCGACCACCACCACCGCCTTTTATGGTCCCACCACGGCCACTTCTACCAGATATGAGGAGACTGATGCCTAGGACCAACATCGAGTATAAGGAGACTGCGGAAGCTCATCACTTCAAGGCCGAGCTTCCGGGTGTCAGAGAAGAGGATGTGACGGTGAACGTAGATGAGGGTGGGAAGATGATCCACATACGTGCCAAATCAAAGCATGGCAGGGAGGAAAACAAAGAGAGATATCATCATGTTGAGCATGGTTGTGGGGAGTTTGCGAGTTCGTTCACGCTACCGCCCGATGCAAATCCTGAGCATATGAGGTGCTGTGTTGATAATGGTGTGCTTACCATCGCCATTCCTAAGCACCAGTCCTACTACCAGTAA

mRNA sequence

ATGCTCAATCCGATTCATTTTCGAGATCGATCTCGAAGGAGAATGCAATCTATGATGCCATATCATCATGGCTTCTTTGGGCGACCTGTTGAAATGTTCGACCCATTCTCCCTCTACTTTCCGAATGATTTTCCCTTCGATTTCCCAGCCTTTCGACCACCACCACCGCCTTTTATGGTCCCACCACGGCCACTTCTACCAGATATGAGGAGACTGATGCCTAGGACCAACATCGAGTATAAGGAGACTGCGGAAGCTCATCACTTCAAGGCCGAGCTTCCGGGTGTCAGAGAAGAGGATGTGACGGTGAACGTAGATGAGGGTGGGAAGATGATCCACATACGTGCCAAATCAAAGCATGGCAGGGAGGAAAACAAAGAGAGATATCATCATGTTGAGCATGGTTGTGGGGAGTTTGCGAGTTCGTTCACGCTACCGCCCGATGCAAATCCTGAGCATATGAGGTGCTGTGTTGATAATGGTGTGCTTACCATCGCCATTCCTAAGCACCAGTCCTACTACCAGTAA

Coding sequence (CDS)

ATGCTCAATCCGATTCATTTTCGAGATCGATCTCGAAGGAGAATGCAATCTATGATGCCATATCATCATGGCTTCTTTGGGCGACCTGTTGAAATGTTCGACCCATTCTCCCTCTACTTTCCGAATGATTTTCCCTTCGATTTCCCAGCCTTTCGACCACCACCACCGCCTTTTATGGTCCCACCACGGCCACTTCTACCAGATATGAGGAGACTGATGCCTAGGACCAACATCGAGTATAAGGAGACTGCGGAAGCTCATCACTTCAAGGCCGAGCTTCCGGGTGTCAGAGAAGAGGATGTGACGGTGAACGTAGATGAGGGTGGGAAGATGATCCACATACGTGCCAAATCAAAGCATGGCAGGGAGGAAAACAAAGAGAGATATCATCATGTTGAGCATGGTTGTGGGGAGTTTGCGAGTTCGTTCACGCTACCGCCCGATGCAAATCCTGAGCATATGAGGTGCTGTGTTGATAATGGTGTGCTTACCATCGCCATTCCTAAGCACCAGTCCTACTACCAGTAA

Protein sequence

MLNPIHFRDRSRRRMQSMMPYHHGFFGRPVEMFDPFSLYFPNDFPFDFPAFRPPPPPFMVPPRPLLPDMRRLMPRTNIEYKETAEAHHFKAELPGVREEDVTVNVDEGGKMIHIRAKSKHGREENKERYHHVEHGCGEFASSFTLPPDANPEHMRCCVDNGVLTIAIPKHQSYYQ

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spo28200.1	Spo28200.1	mRNA

Homology

[Close]

BLAST of Spo28200.1 vs. NCBI nr
Match: gi|902172768|gb|KNA08168.1| (hypothetical protein SOVF_165100 [Spinacia oleracea])

HSP 1 Score: 343.6 bits (880), Expect = 2.100e-91
Identity = 161/161 (100.00%), Postives = 161/161 (100.00%), Query Frame = 1

		  
Query: 15  MQSMMPYHHGFFGRPVEMFDPFSLYFPNDFPFDFPAFRPPPPPFMVPPRPLLPDMRRLMP 74
           MQSMMPYHHGFFGRPVEMFDPFSLYFPNDFPFDFPAFRPPPPPFMVPPRPLLPDMRRLMP
Sbjct: 1   MQSMMPYHHGFFGRPVEMFDPFSLYFPNDFPFDFPAFRPPPPPFMVPPRPLLPDMRRLMP 60

Query: 75  RTNIEYKETAEAHHFKAELPGVREEDVTVNVDEGGKMIHIRAKSKHGREENKERYHHVEH 134
           RTNIEYKETAEAHHFKAELPGVREEDVTVNVDEGGKMIHIRAKSKHGREENKERYHHVEH
Sbjct: 61  RTNIEYKETAEAHHFKAELPGVREEDVTVNVDEGGKMIHIRAKSKHGREENKERYHHVEH 120

Query: 135 GCGEFASSFTLPPDANPEHMRCCVDNGVLTIAIPKHQSYYQ 176
           GCGEFASSFTLPPDANPEHMRCCVDNGVLTIAIPKHQSYYQ
Sbjct: 121 GCGEFASSFTLPPDANPEHMRCCVDNGVLTIAIPKHQSYYQ 161

[Close]

BLAST of Spo28200.1 vs. NCBI nr
Match: gi|870858113|gb|KMT09639.1| (hypothetical protein BVRB_6g130910 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 203.4 bits (516), Expect = 3.300e-49
Identity = 107/168 (63.69%), Postives = 122/168 (72.62%), Query Frame = 1

		  
Query: 15  MQSMMPYH-HGFFGRPVEMFDPFSLYFPNDFPFDFPAFRPPP---PPFMVP-----PRPL 74
           M  MMPYH HGFF RP  MFDPFSL+F +DFP DFP F  PP   PP + P     PRPL
Sbjct: 7   MMPMMPYHDHGFF-RPPTMFDPFSLFFWDDFPCDFPVFGAPPYMAPPLIAPSRPFPPRPL 66

Query: 75  LPDMRRLMPRTNIEYKETAEAHHFKAELPGVREEDVTVNVDEGGKMIHIRAKSKHGREEN 134
           LP MR L+PR  IE+KET EAHHF AELPG+R+EDV V VD+G  M+H+RAK  H +EEN
Sbjct: 67  LPQMRGLVPRAKIEHKETPEAHHFMAELPGMRKEDVVVKVDDGN-MLHVRAKRNHEKEEN 126

Query: 135 KERYHHVEHGCGEFASSFTLPPDANPEHMRCCVDNGVLTIAIPKHQSY 174
           KE YHHVE G GEF S F LPPDA P+ +R CVDNGVLTI IPK + +
Sbjct: 127 KESYHHVERGFGEFVSKFMLPPDAIPQQLRSCVDNGVLTITIPKQKGH 172

[Close]

BLAST of Spo28200.1 vs. NCBI nr
Match: gi|743772273|ref|XP_010916441.1| (PREDICTED: 16.9 kDa class I heat shock protein 2-like [Elaeis guineensis])

HSP 1 Score: 97.8 bits (242), Expect = 2.000e-17
Identity = 52/144 (36.11%), Postives = 82/144 (56.94%), Query Frame = 1

		  
Query: 28  RPVEMFDPFSLYFPNDFPFDFPAFRPPPPPFMVPPRPLLPDMRRLMPRTNIEYKETAEAH 87
           R   ++DPFSL   + F             F+  PRP +           I++KET EAH
Sbjct: 5   RRSNIYDPFSLDVWDPFQ-----------GFLAEPRPGVVSETSAFANARIDWKETPEAH 64

Query: 88  HFKAELPGVREEDVTVNVDEGGKMIHIRAKSKHGREENKERYHHVEHGCGEFASSFTLPP 147
            FKA+LPGV++E+V V V+EG +++ +  +    +EE  +++H VE  CG+F   F LP 
Sbjct: 65  IFKADLPGVKKEEVKVEVEEG-RILKLSGERSKEKEEKNDKWHRVERSCGKFLRRFRLPE 124

Query: 148 DANPEHMRCCVDNGVLTIAIPKHQ 172
           +A  E ++ C++NGVLT+ +PK +
Sbjct: 125 NAKVEQVKACMENGVLTVTVPKEE 136

[Close]

BLAST of Spo28200.1 vs. NCBI nr
Match: gi|255579096|ref|XP_002530396.1| (PREDICTED: 17.3 kDa class I heat shock protein [Ricinus communis])

HSP 1 Score: 96.7 bits (239), Expect = 4.400e-17
Identity = 55/150 (36.67%), Postives = 88/150 (58.67%), Query Frame = 1

		  
Query: 25  FFGRPVEMFDPFSL--YFP-NDFPFDFPAFRPPPPPFMVPPRPLLPDMRRLMPRTNIEYK 84
           F GR   +FDPFSL  + P +DFPF   A        +  PR  + +       T +++K
Sbjct: 9   FGGRRTNIFDPFSLDIWDPFHDFPFTSTA--------LATPRSEIANETSAFANTRMDWK 68

Query: 85  ETAEAHHFKAELPGVREEDVTVNVDEGGKMIHIRAKSKHGREENKERYHHVEHGCGEFAS 144
           ET EAH FKA+LPG+++E+V V ++EG K++ I  +    +EE  +++H VE   G+F  
Sbjct: 69  ETPEAHVFKADLPGLKKEEVKVEIEEG-KVLQISGERSKEKEEKNDKWHRVERSSGKFMR 128

Query: 145 SFTLPPDANPEHMRCCVDNGVLTIAIPKHQ 172
            F LP +A  + ++  ++NGVLT+ +PK +
Sbjct: 129 RFRLPDNAKIDQVKASMENGVLTVTVPKEE 149

[Close]

BLAST of Spo28200.1 vs. NCBI nr
Match: gi|695055233|ref|XP_009415785.1| (PREDICTED: 16.9 kDa class I heat shock protein 2-like [Musa acuminata subsp. malaccensis])

HSP 1 Score: 96.3 bits (238), Expect = 5.700e-17
Identity = 55/146 (37.67%), Postives = 85/146 (58.22%), Query Frame = 1

		  
Query: 28  RPVEMFDPFSLYFPNDFP-FDFPAFRPPPPPFMVPPRPLLPDMRRLMPRTNIEYKETAEA 87
           R   +FDPFSL   + F  F F AFR      +   RP           T I++KET EA
Sbjct: 5   RRSNIFDPFSLDVWDPFQGFPFDAFRS-----LAETRPGFVSETSAFANTRIDWKETPEA 64

Query: 88  HHFKAELPGVREEDVTVNVDEGGKMIHIRAKSKHGREENKERYHHVEHGCGEFASSFTLP 147
           H FKA+LPGV++E+V V V+EG +++ I  +    +EE ++++H VE   G+F   F LP
Sbjct: 65  HVFKADLPGVKKEEVKVEVEEG-RVLQISGERSKEKEEKRDKWHRVERSSGKFLRRFRLP 124

Query: 148 PDANPEHMRCCVDNGVLTIAIPKHQS 173
            +A  + ++  ++NGVLT+ +PK ++
Sbjct: 125 ENAKVDQLKASMENGVLTVTVPKEEA 144

[Close]

BLAST of Spo28200.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QNA4_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_165100 PE=3 SV=1)

HSP 1 Score: 343.6 bits (880), Expect = 1.400e-91
Identity = 161/161 (100.00%), Postives = 161/161 (100.00%), Query Frame = 1

		  
Query: 15  MQSMMPYHHGFFGRPVEMFDPFSLYFPNDFPFDFPAFRPPPPPFMVPPRPLLPDMRRLMP 74
           MQSMMPYHHGFFGRPVEMFDPFSLYFPNDFPFDFPAFRPPPPPFMVPPRPLLPDMRRLMP
Sbjct: 1   MQSMMPYHHGFFGRPVEMFDPFSLYFPNDFPFDFPAFRPPPPPFMVPPRPLLPDMRRLMP 60

Query: 75  RTNIEYKETAEAHHFKAELPGVREEDVTVNVDEGGKMIHIRAKSKHGREENKERYHHVEH 134
           RTNIEYKETAEAHHFKAELPGVREEDVTVNVDEGGKMIHIRAKSKHGREENKERYHHVEH
Sbjct: 61  RTNIEYKETAEAHHFKAELPGVREEDVTVNVDEGGKMIHIRAKSKHGREENKERYHHVEH 120

Query: 135 GCGEFASSFTLPPDANPEHMRCCVDNGVLTIAIPKHQSYYQ 176
           GCGEFASSFTLPPDANPEHMRCCVDNGVLTIAIPKHQSYYQ
Sbjct: 121 GCGEFASSFTLPPDANPEHMRCCVDNGVLTIAIPKHQSYYQ 161

[Close]

BLAST of Spo28200.1 vs. UniProtKB/TrEMBL
Match: A0A0J8CBQ4_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_6g130910 PE=3 SV=1)

HSP 1 Score: 203.4 bits (516), Expect = 2.300e-49
Identity = 107/168 (63.69%), Postives = 122/168 (72.62%), Query Frame = 1

		  
Query: 15  MQSMMPYH-HGFFGRPVEMFDPFSLYFPNDFPFDFPAFRPPP---PPFMVP-----PRPL 74
           M  MMPYH HGFF RP  MFDPFSL+F +DFP DFP F  PP   PP + P     PRPL
Sbjct: 7   MMPMMPYHDHGFF-RPPTMFDPFSLFFWDDFPCDFPVFGAPPYMAPPLIAPSRPFPPRPL 66

Query: 75  LPDMRRLMPRTNIEYKETAEAHHFKAELPGVREEDVTVNVDEGGKMIHIRAKSKHGREEN 134
           LP MR L+PR  IE+KET EAHHF AELPG+R+EDV V VD+G  M+H+RAK  H +EEN
Sbjct: 67  LPQMRGLVPRAKIEHKETPEAHHFMAELPGMRKEDVVVKVDDGN-MLHVRAKRNHEKEEN 126

Query: 135 KERYHHVEHGCGEFASSFTLPPDANPEHMRCCVDNGVLTIAIPKHQSY 174
           KE YHHVE G GEF S F LPPDA P+ +R CVDNGVLTI IPK + +
Sbjct: 127 KESYHHVERGFGEFVSKFMLPPDAIPQQLRSCVDNGVLTITIPKQKGH 172

[Close]

BLAST of Spo28200.1 vs. UniProtKB/TrEMBL
Match: B9SWM7_RICCO (Heat-shock protein, putative OS=Ricinus communis GN=RCOM_0497680 PE=3 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 3.000e-17
Identity = 55/150 (36.67%), Postives = 88/150 (58.67%), Query Frame = 1

		  
Query: 25  FFGRPVEMFDPFSL--YFP-NDFPFDFPAFRPPPPPFMVPPRPLLPDMRRLMPRTNIEYK 84
           F GR   +FDPFSL  + P +DFPF   A        +  PR  + +       T +++K
Sbjct: 9   FGGRRTNIFDPFSLDIWDPFHDFPFTSTA--------LATPRSEIANETSAFANTRMDWK 68

Query: 85  ETAEAHHFKAELPGVREEDVTVNVDEGGKMIHIRAKSKHGREENKERYHHVEHGCGEFAS 144
           ET EAH FKA+LPG+++E+V V ++EG K++ I  +    +EE  +++H VE   G+F  
Sbjct: 69  ETPEAHVFKADLPGLKKEEVKVEIEEG-KVLQISGERSKEKEEKNDKWHRVERSSGKFMR 128

Query: 145 SFTLPPDANPEHMRCCVDNGVLTIAIPKHQ 172
            F LP +A  + ++  ++NGVLT+ +PK +
Sbjct: 129 RFRLPDNAKIDQVKASMENGVLTVTVPKEE 149

[Close]

BLAST of Spo28200.1 vs. UniProtKB/TrEMBL
Match: B9HHJ3_POPTR (18.2 kDa class I heat shock family protein OS=Populus trichocarpa GN=POPTR_0008s06260g PE=3 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 8.900e-17
Identity = 56/150 (37.33%), Postives = 85/150 (56.67%), Query Frame = 1

		  
Query: 25  FFGRPVEMFDPFSL--YFP-NDFPFDFPAFRPPPPPFMVPPRPLLPDMRRLMPRTNIEYK 84
           F GR   +FDPFSL  + P  DFPF   A        +  PR  +         T I++K
Sbjct: 9   FGGRRTNVFDPFSLDIWDPFQDFPFTSTA--------LSAPRSEVAKETSAFASTRIDWK 68

Query: 85  ETAEAHHFKAELPGVREEDVTVNVDEGGKMIHIRAKSKHGREENKERYHHVEHGCGEFAS 144
           ET EAH FKA+LPG+++E+V V ++EG K++ I  +     EE  +++H VE   G+F  
Sbjct: 69  ETPEAHVFKADLPGLKKEEVKVEIEEG-KVLQISGERSKENEEKNDKWHRVERSSGKFLR 128

Query: 145 SFTLPPDANPEHMRCCVDNGVLTIAIPKHQ 172
            F LP +A  + ++  ++NGVLT+ +PK +
Sbjct: 129 RFRLPENAKADQVKASMENGVLTVTVPKEE 149

[Close]

BLAST of Spo28200.1 vs. UniProtKB/TrEMBL
Match: A0A067LC99_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_01353 PE=3 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 1.200e-16
Identity = 56/148 (37.84%), Postives = 85/148 (57.43%), Query Frame = 1

		  
Query: 25  FFGRPVEMFDPFSL--YFP-NDFPFDFPAFRPPPPPFMVPPRPLLPDMRRLMPRTNIEYK 84
           F GR   +FDPFSL  + P  DFPF   A        +  PR  L +       T I++K
Sbjct: 9   FGGRRTNIFDPFSLDIWDPFQDFPFTSTA--------LSAPRSELLNETAAFANTRIDWK 68

Query: 85  ETAEAHHFKAELPGVREEDVTVNVDEGGKMIHIRAKSKHGREENKERYHHVEHGCGEFAS 144
           ET EAH FKA+LPG+++E+V V ++EG +++ I  +    +EE  +++H VE   G+F  
Sbjct: 69  ETPEAHVFKADLPGLKKEEVKVEIEEG-RVLQISGERSKEKEEKNDKWHRVERSSGKFLR 128

Query: 145 SFTLPPDANPEHMRCCVDNGVLTIAIPK 170
            F LP +A    ++  ++NGVLT+ +PK
Sbjct: 129 RFRLPENAKVNEVKASMENGVLTVTVPK 147

[Close]

BLAST of Spo28200.1 vs. ExPASy Swiss-Prot
Match: HSP12_DAUCA (18.0 kDa class I heat shock protein OS=Daucus carota PE=3 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 9.700e-17
Identity = 40/94 (42.55%), Postives = 65/94 (69.15%), Query Frame = 1

		  
Query: 76  TNIEYKETAEAHHFKAELPGVREEDVTVNVDEGGKMIHIRAKSKHGREENKERYHHVEHG 135
           T+I++KET +AH FKA+LPG+++E+V V V+EG K++ I  +    +EE   ++H VE  
Sbjct: 52  THIDWKETPQAHVFKADLPGLKKEEVKVEVEEG-KVLQISGERNKEKEEKNNKWHRVEFS 111

Query: 136 CGEFASSFTLPPDANPEHMRCCVDNGVLTIAIPK 170
            G+F   F LP +AN + ++  ++NGVLT+ +PK
Sbjct: 112 SGKFLRRFRLPENANVDEVKAGMENGVLTVTVPK 144

[Close]

BLAST of Spo28200.1 vs. ExPASy Swiss-Prot
Match: HS17A_ORYSJ (17.9 kDa class I heat shock protein OS=Oryza sativa subsp. japonica GN=HSP17.9A PE=2 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 2.800e-16
Identity = 56/157 (35.67%), Postives = 86/157 (54.78%), Query Frame = 1

		  
Query: 28  RPVEMFDPFSL--YFPND-FPFD---------FPAFRPPPPPFMVPPRPLLPDMRRLMPR 87
           R   +FDPFSL  + P D FPF          FP+F          PR    +       
Sbjct: 5   RRSNVFDPFSLDLWDPFDGFPFGSGGSSSGSIFPSF----------PRGASSETAAFAG- 64

Query: 88  TNIEYKETAEAHHFKAELPGVREEDVTVNVDEGGKMIHIRAKSKHGREENKERYHHVEHG 147
             I++KET EAH FKA++PG+++E+V V VD+G  ++ I  +    +EE  +++H VE  
Sbjct: 65  ARIDWKETPEAHVFKADVPGLKKEEVKVEVDDGN-ILQISGERNKEQEEKTDQWHRVERS 124

Query: 148 CGEFASSFTLPPDANPEHMRCCVDNGVLTIAIPKHQS 173
            G+F   F LP +A PE ++  ++NGVLT+ +PK ++
Sbjct: 125 SGKFLRRFRLPDNAKPEQIKASMENGVLTVTVPKEEA 149

[Close]

BLAST of Spo28200.1 vs. ExPASy Swiss-Prot
Match: HS177_ORYSJ (17.7 kDa class I heat shock protein OS=Oryza sativa subsp. japonica GN=HSP17.7 PE=2 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 2.800e-16
Identity = 41/110 (37.27%), Postives = 66/110 (60.00%), Query Frame = 1

		  
Query: 72  LMPRTN----------IEYKETAEAHHFKAELPGVREEDVTVNVDEGGKMIHIRAKSKHG 131
           L PR N          I++KET E H FKA++PG+++E+V V VD+G  ++ I  +    
Sbjct: 38  LFPRANSDAAAFAGARIDWKETPEVHVFKADVPGLKKEEVKVEVDDGN-ILQISGERSRE 97

Query: 132 REENKERYHHVEHGCGEFASSFTLPPDANPEHMRCCVDNGVLTIAIPKHQ 172
           +EE  +++H VE   G+F   F LP +  PE ++  ++NGVLT+ +PK +
Sbjct: 98  QEEKSDKWHRVERSSGKFLRRFRLPENTKPEQIKASMENGVLTVTVPKEE 146

[Close]

BLAST of Spo28200.1 vs. ExPASy Swiss-Prot
Match: HSP11_PEA (18.1 kDa class I heat shock protein OS=Pisum sativum GN=HSP18.1 PE=2 SV=1)

HSP 1 Score: 85.9 bits (211), Expect = 4.800e-16
Identity = 51/150 (34.00%), Postives = 82/150 (54.67%), Query Frame = 1

		  
Query: 25  FFGRPVEMFDPFSL--YFP-NDFPFDFPAFRPPPPPFMVPPRPLLPDMRRLMPRTNIEYK 84
           F GR   +FDPFSL  + P  DFPF   +           P    P        T +++K
Sbjct: 8   FSGRRSNVFDPFSLDVWDPLKDFPFSNSS-----------PSASFPRENPAFVSTRVDWK 67

Query: 85  ETAEAHHFKAELPGVREEDVTVNVDEGGKMIHIRAKSKHGREENKERYHHVEHGCGEFAS 144
           ET EAH FKA+LPG+++E+V V V++  +++ I  +    +E+  + +H VE   G+F  
Sbjct: 68  ETPEAHVFKADLPGLKKEEVKVEVEDD-RVLQISGERSVEKEDKNDEWHRVERSSGKFLR 127

Query: 145 SFTLPPDANPEHMRCCVDNGVLTIAIPKHQ 172
            F LP +A  + ++  ++NGVLT+ +PK +
Sbjct: 128 RFRLPENAKMDKVKASMENGVLTVTVPKEE 145

[Close]

BLAST of Spo28200.1 vs. ExPASy Swiss-Prot
Match: HSP11_OXYRB (18.3 kDa class I heat shock protein OS=Oxybasis rubra GN=HSP18 PE=2 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 8.200e-16
Identity = 54/156 (34.62%), Postives = 85/156 (54.49%), Query Frame = 1

		  
Query: 17  SMMPYHHGFFGRPVEMFDPFSLYFPNDFPFDFPAFRPPPPPFMVPPRPLLPDMRRLMPRT 76
           S++P +    GR   +FDPFSL    D  +D   F   P      PR             
Sbjct: 2   SLIPNNWFNTGRRSNIFDPFSL----DEIWD--PFFGLPSTLSTVPRSETAAETAAFANA 61

Query: 77  NIEYKETAEAHHFKAELPGVREEDVTVNVDEGGKMIHIRAKSKHGREENKERYHHVEHGC 136
            I++KET EAH FKA+LPGV++E+V V V++G  ++ I  +    +EE  + +H VE   
Sbjct: 62  RIDWKETPEAHVFKADLPGVKKEEVKVEVEDGN-VLRISGQRAREKEEKNDTWHRVERSS 121

Query: 137 GEFASSFTLPPDANPEHMRCCVDNGVLTIAIPKHQS 173
           G+F   F LP +A  + ++  ++NGVLT+ +PK+++
Sbjct: 122 GQFMRKFRLPENAKVDQVKAGMENGVLTVTVPKNEA 150

[Close]

BLAST of Spo28200.1 vs. TAIR (Arabidopsis)
Match: AT1G53540.1 (HSP20-like chaperones superfamily protein)

HSP 1 Score: 82.0 bits (201), Expect = 3.900e-16
Identity = 50/145 (34.48%), Postives = 78/145 (53.79%), Query Frame = 1

		  
Query: 25  FFGRPVEMFDPFSLYFPNDFPFDFPAFRPPPPPFMVPPRPLLPDMRRLMPRTNIEYKETA 84
           F GR   +FDPFSL    D    F  F  P      P   +            ++++ET 
Sbjct: 8   FGGRRTNVFDPFSL----DVFDPFEGFLTPSGLANAPAMDVAA-----FTNAKVDWRETP 67

Query: 85  EAHHFKAELPGVREEDVTVNVDEGGKMIHIRAKSKHGREENKERYHHVEHGCGEFASSFT 144
           EAH FKA+LPG+R+E+V V V++G  ++ I  +  +  EE  +++H VE   G+F   F 
Sbjct: 68  EAHVFKADLPGLRKEEVKVEVEDGN-ILQISGERSNENEEKNDKWHRVERSSGKFTRRFR 127

Query: 145 LPPDANPEHMRCCVDNGVLTIAIPK 170
           LP +A  E ++  ++NGVL++ +PK
Sbjct: 128 LPENAKMEEIKASMENGVLSVTVPK 142

[Close]

BLAST of Spo28200.1 vs. TAIR (Arabidopsis)
Match: AT1G07400.1 (HSP20-like chaperones superfamily protein)

HSP 1 Score: 80.1 bits (196), Expect = 1.500e-15
Identity = 48/154 (31.17%), Postives = 82/154 (53.25%), Query Frame = 1

		  
Query: 17  SMMPYHHGFFGRPVEMFDPFSLYFPNDFP-FDFPAFRPPPPPFMVPPRPLLPDMRRLMPR 76
           S++P   G   R   +FDPFSL   + F    FP+               L      +  
Sbjct: 2   SLIPSFFGNNRRSNSIFDPFSLDVWDPFKELQFPSS--------------LSGETSAITN 61

Query: 77  TNIEYKETAEAHHFKAELPGVREEDVTVNVDEGGKMIHIRAKSKHGREENKERYHHVEHG 136
             +++KETAEAH FKA+LPG+++E+V V +++   ++ I  +    +EE ++ +H VE  
Sbjct: 62  ARVDWKETAEAHVFKADLPGMKKEEVKVEIEDDS-VLKISGERHVEKEEKQDTWHRVERS 121

Query: 137 CGEFASSFTLPPDANPEHMRCCVDNGVLTIAIPK 170
            G+F+  F LP +   + ++  ++NGVLT+ +PK
Sbjct: 122 SGQFSRKFKLPENVKMDQVKASMENGVLTVTVPK 140

[Close]

BLAST of Spo28200.1 vs. TAIR (Arabidopsis)
Match: AT3G46230.1 (heat shock protein 17.4)

HSP 1 Score: 76.6 bits (187), Expect = 1.700e-14
Identity = 48/148 (32.43%), Postives = 77/148 (52.03%), Query Frame = 1

		  
Query: 25  FFGRPVEMFDPFSLYFPNDFP-FDFPAFRPPPPPFMVPPRPLLPDMRRLMPRTNIEYKET 84
           F GR   +FDPFSL   + F  F  P     P   +                  ++++ET
Sbjct: 8   FGGRRTNVFDPFSLDVWDPFEGFLTPGLTNAPAKDVAA-----------FTNAKVDWRET 67

Query: 85  AEAHHFKAELPGVREEDVTVNVDEGGKMIHIRAKSKHGREENKERYHHVEHGCGEFASSF 144
            EAH FKA++PG+++E+V V V++G  ++ I  +     EE  + +H VE   G+F   F
Sbjct: 68  PEAHVFKADVPGLKKEEVKVEVEDGN-ILQISGERSSENEEKSDTWHRVERSSGKFMRRF 127

Query: 145 TLPPDANPEHMRCCVDNGVLTIAIPKHQ 172
            LP +A  E ++  ++NGVL++ +PK Q
Sbjct: 128 RLPENAKVEEVKASMENGVLSVTVPKVQ 143

[Close]

BLAST of Spo28200.1 vs. TAIR (Arabidopsis)
Match: AT1G59860.1 (HSP20-like chaperones superfamily protein)

HSP 1 Score: 75.5 bits (184), Expect = 3.700e-14
Identity = 43/139 (30.94%), Postives = 74/139 (53.24%), Query Frame = 1

		  
Query: 32  MFDPFSLYFPNDFP-FDFPAFRPPPPPFMVPPRPLLPDMRRLMPRTNIEYKETAEAHHFK 91
           +FDPFSL   + F    FP+                      +    +++KETAEAH FK
Sbjct: 18  IFDPFSLDVWDPFKELQFPS-----------------SSSSAIANARVDWKETAEAHVFK 77

Query: 92  AELPGVREEDVTVNVDEGGKMIHIRAKSKHGREENKERYHHVEHGCGEFASSFTLPPDAN 151
           A+LPG+++E+V V +++   ++ I  +    +EE ++ +H VE   G F+  F LP +  
Sbjct: 78  ADLPGMKKEEVKVEIEDDS-VLKISGERHVEKEEKQDTWHRVERSSGGFSRKFRLPENVK 137

Query: 152 PEHMRCCVDNGVLTIAIPK 170
            + ++  ++NGVLT+ +PK
Sbjct: 138 MDQVKASMENGVLTVTVPK 138

[Close]

BLAST of Spo28200.1 vs. TAIR (Arabidopsis)
Match: AT5G59720.1 (heat shock protein 18.2)

HSP 1 Score: 75.5 bits (184), Expect = 3.700e-14
Identity = 51/147 (34.69%), Postives = 77/147 (52.38%), Query Frame = 1

		  
Query: 25  FFGRPVEMFDPFSLYFPNDFPFDFPAFRPPPPPFMVPPRPLLPDMRRLMPRTN--IEYKE 84
           F GR   +FDPFS     D    F  F  P               R +   TN  +++KE
Sbjct: 8   FGGRRSNVFDPFS----QDLWDPFEGFFTPSSALANASTA-----RDVAAFTNARVDWKE 67

Query: 85  TAEAHHFKAELPGVREEDVTVNVDEGGKMIHIRAKSKHGREENKERYHHVEHGCGEFASS 144
           T EAH FKA+LPG+++E+V V V++   ++ I  +     EE  +++H VE   G+F   
Sbjct: 68  TPEAHVFKADLPGLKKEEVKVEVEDKN-VLQISGERSKENEEKNDKWHRVERASGKFMRR 127

Query: 145 FTLPPDANPEHMRCCVDNGVLTIAIPK 170
           F LP +A  E ++  ++NGVLT+ +PK
Sbjct: 128 FRLPENAKMEEVKATMENGVLTVVVPK 144

The following BLAST results are available for this feature:

BLAST of Spo28200.1 vs. NCBI nr
BLAST of Spo28200.1 vs. UniProtKB/TrEMBL
BLAST of Spo28200.1 vs. ExPASy Swiss-Prot
BLAST of Spo28200.1 vs. TAIR (Arabidopsis)

BLAST of Spo28200.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5

Position :

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Match Name	E-value	Identity	Description
gi\|902172768\|gb\|KNA08168.1\|	2.1e-91	100.	hypothetical protein SOVF_1651...	[more]
gi\|870858113\|gb\|KMT09639.1\|	3.3e-49	63.6	hypothetical protein BVRB_6g13...	[more]
gi\|743772273\|ref\|XP_010916441.1\|	2.0e-17	36.1	PREDICTED: 16.9 kDa class I he...	[more]
gi\|255579096\|ref\|XP_002530396.1\|	4.4e-17	36.6	PREDICTED: 17.3 kDa class I he...	[more]
gi\|695055233\|ref\|XP_009415785.1\|	5.7e-17	37.6	PREDICTED: 16.9 kDa class I he...	[more]

BLAST of Spo28200.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5

Position :

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Match Name	E-value	Identity	Description
A0A0K9QNA4_SPIOL	1.4e-91	100.	Uncharacterized protein OS=Spi...	[more]
A0A0J8CBQ4_BETVU	2.3e-49	63.6	Uncharacterized protein OS=Bet...	[more]
B9SWM7_RICCO	3.0e-17	36.6	Heat-shock protein, putative O...	[more]
B9HHJ3_POPTR	8.9e-17	37.3	18.2 kDa class I heat shock fa...	[more]
A0A067LC99_JATCU	1.2e-16	37.8	Uncharacterized protein OS=Jat...	[more]

BLAST of Spo28200.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5

Position :

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Match Name	E-value	Identity	Description
HSP12_DAUCA	9.7e-17	42.5	18.0 kDa class I heat shock pr...	[more]
HS17A_ORYSJ	2.8e-16	35.6	17.9 kDa class I heat shock pr...	[more]
HS177_ORYSJ	2.8e-16	37.2	17.7 kDa class I heat shock pr...	[more]
HSP11_PEA	4.8e-16	34.0	18.1 kDa class I heat shock pr...	[more]
HSP11_OXYRB	8.2e-16	34.6	18.3 kDa class I heat shock pr...	[more]

BLAST of Spo28200.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 5

Position :

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Match Name	E-value	Identity	Description
AT1G53540.1	3.9e-16	34.4	HSP20-like chaperones superfam...	[more]
AT1G07400.1	1.5e-15	31.1	HSP20-like chaperones superfam...	[more]
AT3G46230.1	1.7e-14	32.4	heat shock protein 17.4	[more]
AT1G59860.1	3.7e-14	30.9	HSP20-like chaperones superfam...	[more]
AT5G59720.1	3.7e-14	34.6	heat shock protein 18.2	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002068	Alpha crystallin/Hsp20 domain	PFAM	PF00011	HSP20	coord: 80..172 score: 2.4
IPR002068	Alpha crystallin/Hsp20 domain	PROFILE	PS01031	HSP20	coord: 79..169 score: 18
IPR008978	HSP20-like chaperone	GENE3D	2.60.40.790		coord: 26..171 score: 9.2
IPR008978	HSP20-like chaperone	unknown	SSF49764	HSP20-like chaperones	coord: 53..171 score: 1.15

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009408	response to heat
biological_process	GO:0000271	polysaccharide biosynthetic process
biological_process	GO:0006284	base-excision repair
biological_process	GO:0006355	regulation of transcription, DNA-templated
biological_process	GO:0006084	acetyl-CoA metabolic process
biological_process	GO:0048767	root hair elongation
biological_process	GO:0010075	regulation of meristem growth
biological_process	GO:0006090	pyruvate metabolic process
biological_process	GO:0016126	sterol biosynthetic process
biological_process	GO:0009664	plant-type cell wall organization
biological_process	GO:0048653	anther development
biological_process	GO:0009825	multidimensional cell growth
biological_process	GO:0006633	fatty acid biosynthetic process
biological_process	GO:0009932	cell tip growth
biological_process	GO:0016132	brassinosteroid biosynthetic process
biological_process	GO:0009832	plant-type cell wall biogenesis
biological_process	GO:0043481	anthocyanin accumulation in tissues in response to UV light
cellular_component	GO:0009317	acetyl-CoA carboxylase complex
cellular_component	GO:0009941	chloroplast envelope
cellular_component	GO:0009570	chloroplast stroma
cellular_component	GO:0005667	transcription factor complex
molecular_function	GO:0052821	DNA-7-methyladenine glycosylase activity
molecular_function	GO:0052822	DNA-3-methylguanine glycosylase activity
molecular_function	GO:0008725	DNA-3-methyladenine glycosylase activity
molecular_function	GO:0009374	biotin binding
molecular_function	GO:0003700	DNA-binding transcription factor activity
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003989	acetyl-CoA carboxylase activity
molecular_function	GO:0043916	DNA-7-methylguanine glycosylase activity

RNA-Seq Expression

Co-expression


Gene	r value	Expression
Spo21412	0.69	Barchart \| Table
Spo18143	0.68	Barchart \| Table
Spo15092	0.67	Barchart \| Table
Spo23067	0.67	Barchart \| Table
Spo23874	0.66	Barchart \| Table
Spo21414	0.65	Barchart \| Table