Spo28224 (gene) | SpinachBase

Overview


Name	Spo28224
Type	gene
Organism	Spinacia oleracea (Spinach)
Description	Chitinase
Location	chr1 : 5556175 .. 5556414 (+)

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATGAAGGTTAAGAGCTTTGTGATTATCATGGTGATCATGACCTTGTTGGTTGGAACATCAATGGCACAACAAGGTGCATGTAACAATGGTCATTGCCCCGCAGGACTTTGTTGTAGCCGATTCAACTTCTGTGGTAGTGGACCGGCATATTGTGGTGGTGCTGAGGAGCAGGCGGAAGCTTATCACCCATCTACTGATGTTCCTCAAACTCGCAAGGTTGCTGCTGGTGCTCCTTGA

mRNA sequence

ATGATGAAGGTTAAGAGCTTTGTGATTATCATGGTGATCATGACCTTGTTGGTTGGAACATCAATGGCACAACAAGGTGCATGTAACAATGGTCATTGCCCCGCAGGACTTTGTTGTAGCCGATTCAACTTCTGTGGTAGTGGACCGGCATATTGTGGTGGTGCTGAGGAGCAGGCGGAAGCTTATCACCCATCTACTGATGTTCCTCAAACTCGCAAGGTTGCTGCTGGTGCTCCTTGA

Coding sequence (CDS)

ATGATGAAGGTTAAGAGCTTTGTGATTATCATGGTGATCATGACCTTGTTGGTTGGAACATCAATGGCACAACAAGGTGCATGTAACAATGGTCATTGCCCCGCAGGACTTTGTTGTAGCCGATTCAACTTCTGTGGTAGTGGACCGGCATATTGTGGTGGTGCTGAGGAGCAGGCGGAAGCTTATCACCCATCTACTGATGTTCCTCAAACTCGCAAGGTTGCTGCTGGTGCTCCTTGA

Protein sequence

MMKVKSFVIIMVIMTLLVGTSMAQQGACNNGHCPAGLCCSRFNFCGSGPAYCGGAEEQAEAYHPSTDVPQTRKVAAGAP

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spo28224.1	Spo28224.1	mRNA

Homology

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BLAST of Spo28224.1 vs. NCBI nr
Match: gi|902235484|gb|KNA23935.1| (hypothetical protein SOVF_020890 [Spinacia oleracea])

HSP 1 Score: 151.4 bits (381), Expect = 6.700e-34
Identity = 76/79 (96.20%), Postives = 76/79 (96.20%), Query Frame = 1

		  
Query: 1  MMKVKSFVIIMVIMTLLVGTSMAQQGACNNGHCPAGLCCSRFNFCGSGPAYCGGAEEQAE 60
          MMKVKSFVIIMVIMTLLVGTSMAQQGACNNGHCPAGLCCSRFNFCGSGPAYCGGAEEQ E
Sbjct: 1  MMKVKSFVIIMVIMTLLVGTSMAQQGACNNGHCPAGLCCSRFNFCGSGPAYCGGAEEQVE 60

Query: 61 AYHPSTDVPQTRKVAAGAP 80
          A HPSTDVPQTRKV AGAP
Sbjct: 61 A-HPSTDVPQTRKVVAGAP 78

[Close]

BLAST of Spo28224.1 vs. NCBI nr
Match: gi|902178481|gb|KNA09156.1| (hypothetical protein SOVF_156110 [Spinacia oleracea])

HSP 1 Score: 148.3 bits (373), Expect = 5.700e-33
Identity = 74/79 (93.67%), Postives = 75/79 (94.94%), Query Frame = 1

		  
Query: 1  MMKVKSFVIIMVIMTLLVGTSMAQQGACNNGHCPAGLCCSRFNFCGSGPAYCGGAEEQAE 60
          M+KVKSFVIIMVIMTLLVGTSMAQQGACNNGHCPAGLCCSRFNFCGSGPAYCGGAEEQ E
Sbjct: 1  MIKVKSFVIIMVIMTLLVGTSMAQQGACNNGHCPAGLCCSRFNFCGSGPAYCGGAEEQVE 60

Query: 61 AYHPSTDVPQTRKVAAGAP 80
          A HPSTDVPQTRKV  GAP
Sbjct: 61 A-HPSTDVPQTRKVVVGAP 78

[Close]

BLAST of Spo28224.1 vs. NCBI nr
Match: gi|902212711|gb|KNA17066.1| (hypothetical protein SOVF_083390 [Spinacia oleracea])

HSP 1 Score: 147.1 bits (370), Expect = 1.300e-32
Identity = 73/79 (92.41%), Postives = 76/79 (96.20%), Query Frame = 1

		  
Query: 1  MMKVKSFVIIMVIMTLLVGTSMAQQGACNNGHCPAGLCCSRFNFCGSGPAYCGGAEEQAE 60
          MMKVKSFVIIMV+MTLLVGTSMAQQGACNNGHC AGLCCS+FNFCGSGPAYCGGAEEQ +
Sbjct: 1  MMKVKSFVIIMVVMTLLVGTSMAQQGACNNGHCAAGLCCSKFNFCGSGPAYCGGAEEQVQ 60

Query: 61 AYHPSTDVPQTRKVAAGAP 80
          A HPSTDVPQTRKVAAGAP
Sbjct: 61 A-HPSTDVPQTRKVAAGAP 78

[Close]

BLAST of Spo28224.1 vs. NCBI nr
Match: gi|902232157|gb|KNA22524.1| (hypothetical protein SOVF_033220 [Spinacia oleracea])

HSP 1 Score: 144.8 bits (364), Expect = 6.300e-32
Identity = 72/76 (94.74%), Postives = 72/76 (94.74%), Query Frame = 1

		  
Query: 4   VKSFVIIMVIMTLLVGTSMAQQGACNNGHCPAGLCCSRFNFCGSGPAYCGGAEEQAEAYH 63
           VKSFVIIMVIMTLLVGTSMAQQGACNNGHCPAGLCCSRFNFCGSGPAYCGGAEEQ EA H
Sbjct: 353 VKSFVIIMVIMTLLVGTSMAQQGACNNGHCPAGLCCSRFNFCGSGPAYCGGAEEQVEA-H 412

Query: 64  PSTDVPQTRKVAAGAP 80
           PSTDVPQTRKV  GAP
Sbjct: 413 PSTDVPQTRKVVVGAP 427

[Close]

BLAST of Spo28224.1 vs. NCBI nr
Match: gi|902208147|gb|KNA15864.1| (hypothetical protein SOVF_094440 [Spinacia oleracea])

HSP 1 Score: 83.6 bits (205), Expect = 1.700e-13
Identity = 43/74 (58.11%), Postives = 55/74 (74.32%), Query Frame = 1

		  
Query: 4  VKSFVIIMVIMTLLVGTSMAQQGACNN-GHCPAGLCCSRFNFCGSGPAYCGGAEEQAEAY 63
          +K FVI+MV+M+++VGTS+A  G CN  G CPAG CCS++ +CG GPAYCG AE+Q  A+
Sbjct: 2  MKMFVIVMVMMSMMVGTSIAS-GECNMYGRCPAGYCCSKYGYCGLGPAYCGDAEQQEAAH 61

Query: 64 HPSTDVPQTRKVAA 77
               VPQTRKV A
Sbjct: 62 ATINVVPQTRKVRA 74

[Close]

BLAST of Spo28224.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RWM2_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_020890 PE=4 SV=1)

HSP 1 Score: 151.4 bits (381), Expect = 4.700e-34
Identity = 76/79 (96.20%), Postives = 76/79 (96.20%), Query Frame = 1

		  
Query: 1  MMKVKSFVIIMVIMTLLVGTSMAQQGACNNGHCPAGLCCSRFNFCGSGPAYCGGAEEQAE 60
          MMKVKSFVIIMVIMTLLVGTSMAQQGACNNGHCPAGLCCSRFNFCGSGPAYCGGAEEQ E
Sbjct: 1  MMKVKSFVIIMVIMTLLVGTSMAQQGACNNGHCPAGLCCSRFNFCGSGPAYCGGAEEQVE 60

Query: 61 AYHPSTDVPQTRKVAAGAP 80
          A HPSTDVPQTRKV AGAP
Sbjct: 61 A-HPSTDVPQTRKVVAGAP 78

[Close]

BLAST of Spo28224.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QRN5_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_156110 PE=4 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 4.000e-33
Identity = 74/79 (93.67%), Postives = 75/79 (94.94%), Query Frame = 1

		  
Query: 1  MMKVKSFVIIMVIMTLLVGTSMAQQGACNNGHCPAGLCCSRFNFCGSGPAYCGGAEEQAE 60
          M+KVKSFVIIMVIMTLLVGTSMAQQGACNNGHCPAGLCCSRFNFCGSGPAYCGGAEEQ E
Sbjct: 1  MIKVKSFVIIMVIMTLLVGTSMAQQGACNNGHCPAGLCCSRFNFCGSGPAYCGGAEEQVE 60

Query: 61 AYHPSTDVPQTRKVAAGAP 80
          A HPSTDVPQTRKV  GAP
Sbjct: 61 A-HPSTDVPQTRKVVVGAP 78

[Close]

BLAST of Spo28224.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RC51_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_083390 PE=4 SV=1)

HSP 1 Score: 147.1 bits (370), Expect = 8.900e-33
Identity = 73/79 (92.41%), Postives = 76/79 (96.20%), Query Frame = 1

		  
Query: 1  MMKVKSFVIIMVIMTLLVGTSMAQQGACNNGHCPAGLCCSRFNFCGSGPAYCGGAEEQAE 60
          MMKVKSFVIIMV+MTLLVGTSMAQQGACNNGHC AGLCCS+FNFCGSGPAYCGGAEEQ +
Sbjct: 1  MMKVKSFVIIMVVMTLLVGTSMAQQGACNNGHCAAGLCCSKFNFCGSGPAYCGGAEEQVQ 60

Query: 61 AYHPSTDVPQTRKVAAGAP 80
          A HPSTDVPQTRKVAAGAP
Sbjct: 61 A-HPSTDVPQTRKVAAGAP 78

[Close]

BLAST of Spo28224.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RUB6_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_033220 PE=4 SV=1)

HSP 1 Score: 144.8 bits (364), Expect = 4.400e-32
Identity = 72/76 (94.74%), Postives = 72/76 (94.74%), Query Frame = 1

		  
Query: 4   VKSFVIIMVIMTLLVGTSMAQQGACNNGHCPAGLCCSRFNFCGSGPAYCGGAEEQAEAYH 63
           VKSFVIIMVIMTLLVGTSMAQQGACNNGHCPAGLCCSRFNFCGSGPAYCGGAEEQ EA H
Sbjct: 353 VKSFVIIMVIMTLLVGTSMAQQGACNNGHCPAGLCCSRFNFCGSGPAYCGGAEEQVEA-H 412

Query: 64  PSTDVPQTRKVAAGAP 80
           PSTDVPQTRKV  GAP
Sbjct: 413 PSTDVPQTRKVVVGAP 427

[Close]

BLAST of Spo28224.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RA27_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_094440 PE=4 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 1.200e-13
Identity = 43/74 (58.11%), Postives = 55/74 (74.32%), Query Frame = 1

		  
Query: 4  VKSFVIIMVIMTLLVGTSMAQQGACNN-GHCPAGLCCSRFNFCGSGPAYCGGAEEQAEAY 63
          +K FVI+MV+M+++VGTS+A  G CN  G CPAG CCS++ +CG GPAYCG AE+Q  A+
Sbjct: 2  MKMFVIVMVMMSMMVGTSIAS-GECNMYGRCPAGYCCSKYGYCGLGPAYCGDAEQQEAAH 61

Query: 64 HPSTDVPQTRKVAA 77
               VPQTRKV A
Sbjct: 62 ATINVVPQTRKVRA 74

[Close]

BLAST of Spo28224.1 vs. ExPASy Swiss-Prot
Match: AMP_AMACA (Antimicrobial peptide 2 OS=Amaranthus caudatus PE=1 SV=2)

HSP 1 Score: 54.3 bits (129), Expect = 7.000e-7
Identity = 31/81 (38.27%), Postives = 45/81 (55.56%), Query Frame = 1

		  
Query: 2  MKVKSFVIIMVIMTLLVGTSMAQQGACNNGHCPAGLCCSRFNFCGSGPAYCGGA----EE 61
          MK  + ++I+++  ++V  SM   G C  G CP+G+CCS+F +CG GP YCG A    + 
Sbjct: 4  MKCVALIVIVMMAFMMVDPSMGV-GECVRGRCPSGMCCSQFGYCGKGPKYCGRASTTVDH 63

Query: 62 QAEAYHPSTDVPQTRKVAAGA 79
          QA+     T    T    AGA
Sbjct: 64 QADVAATKTAKNPTDAKLAGA 83

[Close]

BLAST of Spo28224.1 vs. ExPASy Swiss-Prot
Match: AMP_AMARE (Antimicrobial peptide Ar-AMP OS=Amaranthus retroflexus PE=1 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 9.200e-7
Identity = 29/67 (43.28%), Postives = 43/67 (64.18%), Query Frame = 1

		  
Query: 1  MMKVKSFVIIMVIMT--LLVGTSMAQQGACNNGHCPAGLCCSRFNFCGSGPAYCGGA--- 60
          M+ +KS  +I+++M   ++V  SM   G C  G CP+G+CCS+F +CG GP YCG A   
Sbjct: 1  MVNMKSVALIVIVMMAFMMVDPSMGA-GECVQGRCPSGMCCSQFGYCGRGPKYCGRASTT 60

Query: 61 -EEQAEA 62
           + QA+A
Sbjct: 61 VDHQADA 66

The following BLAST results are available for this feature:

BLAST of Spo28224.1 vs. NCBI nr
BLAST of Spo28224.1 vs. UniProtKB/TrEMBL
BLAST of Spo28224.1 vs. ExPASy Swiss-Prot
BLAST of Spo28224.1 vs. TAIR (Arabidopsis)

BLAST of Spo28224.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5

Position :

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x 1


Match Name	E-value	Identity	Description
gi\|902235484\|gb\|KNA23935.1\|	6.7e-34	96.2	hypothetical protein SOVF_0208...	[more]
gi\|902178481\|gb\|KNA09156.1\|	5.7e-33	93.6	hypothetical protein SOVF_1561...	[more]
gi\|902212711\|gb\|KNA17066.1\|	1.3e-32	92.4	hypothetical protein SOVF_0833...	[more]
gi\|902232157\|gb\|KNA22524.1\|	6.3e-32	94.7	hypothetical protein SOVF_0332...	[more]
gi\|902208147\|gb\|KNA15864.1\|	1.7e-13	58.1	hypothetical protein SOVF_0944...	[more]

BLAST of Spo28224.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5

Position :

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x 1


Match Name	E-value	Identity	Description
A0A0K9RWM2_SPIOL	4.7e-34	96.2	Uncharacterized protein OS=Spi...	[more]
A0A0K9QRN5_SPIOL	4.0e-33	93.6	Uncharacterized protein OS=Spi...	[more]
A0A0K9RC51_SPIOL	8.9e-33	92.4	Uncharacterized protein OS=Spi...	[more]
A0A0K9RUB6_SPIOL	4.4e-32	94.7	Uncharacterized protein OS=Spi...	[more]
A0A0K9RA27_SPIOL	1.2e-13	58.1	Uncharacterized protein OS=Spi...	[more]

BLAST of Spo28224.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 2

Position :

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x 1


Match Name	E-value	Identity	Description
AMP_AMACA	7.0e-7	38.2	Antimicrobial peptide 2 OS=Ama...	[more]
AMP_AMARE	9.2e-7	43.2	Antimicrobial peptide Ar-AMP O...	[more]

BLAST of Spo28224.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 0

Position :

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x 1


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001002	Chitin-binding, type 1	GENE3D	3.30.60.10		coord: 28..55 score: 2.
IPR001002	Chitin-binding, type 1	PFAM	PF00187	Chitin_bind_1	coord: 29..53 score: 6.
IPR001002	Chitin-binding, type 1	SMART	SM00270	ChitinBD_3	coord: 27..60 score: 1.
IPR001002	Chitin-binding, type 1	unknown	SSF57016	Plant lectins/antimicrobial peptides	coord: 28..54 score: 7.3

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0050832	defense response to fungus
biological_process	GO:0050830	defense response to Gram-positive bacterium
biological_process	GO:0005982	starch metabolic process
biological_process	GO:0005985	sucrose metabolic process
biological_process	GO:0008152	metabolic process
molecular_function	GO:0008061	chitin binding
molecular_function	GO:0042973	glucan endo-1,3-beta-D-glucosidase activity
molecular_function	GO:0005515	protein binding
molecular_function	GO:0016740	transferase activity

RNA-Seq Expression

Co-expression


Gene	r value	Expression
Spo28225	0.99	Barchart \| Table
Spo28227	0.95	Barchart \| Table
Spo02607	0.74	Barchart \| Table
Spo08170	0.73	Barchart \| Table
Spo10790	0.73	Barchart \| Table
Spo00732	0.68	Barchart \| Table
Spo15600	0.67	Barchart \| Table
Spo28474	0.66	Barchart \| Table