Spo28225 (gene) | SpinachBase

Overview


Name	Spo28225
Type	gene
Organism	Spinacia oleracea (Spinach)
Description	Endochitinase (3.2.1.14) (Precursor)
Location	chr1 : 5616523 .. 5616922 (+)

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AGATTAAAAGAAAAGTCGATCGACATAATGTAAAGCTATAGCTCGCTCGTTATATTGATCTATCTATCTATAAATAGCCTTCACTTATGTTAGTATTTTACACCAAAAACAACAACAAAAACAATAAATAATTAAAAAGAAAGAGCTTTAATTTGTAATAAAAATGATGAAGGTTAAGAGCTTTGTGATTATCATGGTGATCATGACCTTGTTGGTTGGAACATCAATGGCACAACAAGGTGCATGTAACAATGGTCATTGCCCCGCAGGACTTTGTTGTAGCCGATTCAACTTCTGTGGTAGTGGACCGGCATATTGTGGTGGTGCTGAGGAGCAGGTGGAAGCTCACCCATCTACTGATGTTCCTCAAACTCGCAAGGTTGTTGTTGGTGCTCCTTGA

mRNA sequence

AGATTAAAAGAAAAGTCGATCGACATAATGTAAAGCTATAGCTCGCTCGTTATATTGATCTATCTATCTATAAATAGCCTTCACTTATGTTAGTATTTTACACCAAAAACAACAACAAAAACAATAAATAATTAAAAAGAAAGAGCTTTAATTTGTAATAAAAATGATGAAGGTTAAGAGCTTTGTGATTATCATGGTGATCATGACCTTGTTGGTTGGAACATCAATGGCACAACAAGGTGCATGTAACAATGGTCATTGCCCCGCAGGACTTTGTTGTAGCCGATTCAACTTCTGTGGTAGTGGACCGGCATATTGTGGTGGTGCTGAGGAGCAGGTGGAAGCTCACCCATCTACTGATGTTCCTCAAACTCGCAAGGTTGTTGTTGGTGCTCCTTGA

Coding sequence (CDS)

ATGATGAAGGTTAAGAGCTTTGTGATTATCATGGTGATCATGACCTTGTTGGTTGGAACATCAATGGCACAACAAGGTGCATGTAACAATGGTCATTGCCCCGCAGGACTTTGTTGTAGCCGATTCAACTTCTGTGGTAGTGGACCGGCATATTGTGGTGGTGCTGAGGAGCAGGTGGAAGCTCACCCATCTACTGATGTTCCTCAAACTCGCAAGGTTGTTGTTGGTGCTCCTTGA

Protein sequence

MMKVKSFVIIMVIMTLLVGTSMAQQGACNNGHCPAGLCCSRFNFCGSGPAYCGGAEEQVEAHPSTDVPQTRKVVVGAP

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spo28225.1	Spo28225.1	mRNA

Homology

[Close]

BLAST of Spo28225.1 vs. NCBI nr
Match: gi|902178481|gb|KNA09156.1| (hypothetical protein SOVF_156110 [Spinacia oleracea])

HSP 1 Score: 159.1 bits (401), Expect = 3.200e-36
Identity = 77/78 (98.72%), Postives = 78/78 (100.00%), Query Frame = 1


		  
Query: 1  MMKVKSFVIIMVIMTLLVGTSMAQQGACNNGHCPAGLCCSRFNFCGSGPAYCGGAEEQVE 60
          M+KVKSFVIIMVIMTLLVGTSMAQQGACNNGHCPAGLCCSRFNFCGSGPAYCGGAEEQVE
Sbjct: 1  MIKVKSFVIIMVIMTLLVGTSMAQQGACNNGHCPAGLCCSRFNFCGSGPAYCGGAEEQVE 60

Query: 61 AHPSTDVPQTRKVVVGAP 79
          AHPSTDVPQTRKVVVGAP
Sbjct: 61 AHPSTDVPQTRKVVVGAP 78

[Close]

BLAST of Spo28225.1 vs. NCBI nr
Match: gi|902235484|gb|KNA23935.1| (hypothetical protein SOVF_020890 [Spinacia oleracea])

HSP 1 Score: 159.1 bits (401), Expect = 3.200e-36
Identity = 77/78 (98.72%), Postives = 77/78 (98.72%), Query Frame = 1


		  
Query: 1  MMKVKSFVIIMVIMTLLVGTSMAQQGACNNGHCPAGLCCSRFNFCGSGPAYCGGAEEQVE 60
          MMKVKSFVIIMVIMTLLVGTSMAQQGACNNGHCPAGLCCSRFNFCGSGPAYCGGAEEQVE
Sbjct: 1  MMKVKSFVIIMVIMTLLVGTSMAQQGACNNGHCPAGLCCSRFNFCGSGPAYCGGAEEQVE 60

Query: 61 AHPSTDVPQTRKVVVGAP 79
          AHPSTDVPQTRKVV GAP
Sbjct: 61 AHPSTDVPQTRKVVAGAP 78

[Close]

BLAST of Spo28225.1 vs. NCBI nr
Match: gi|902232157|gb|KNA22524.1| (hypothetical protein SOVF_033220 [Spinacia oleracea])

HSP 1 Score: 156.0 bits (393), Expect = 2.700e-35
Identity = 75/75 (100.00%), Postives = 75/75 (100.00%), Query Frame = 1


		  
Query: 4   VKSFVIIMVIMTLLVGTSMAQQGACNNGHCPAGLCCSRFNFCGSGPAYCGGAEEQVEAHP 63
           VKSFVIIMVIMTLLVGTSMAQQGACNNGHCPAGLCCSRFNFCGSGPAYCGGAEEQVEAHP
Sbjct: 353 VKSFVIIMVIMTLLVGTSMAQQGACNNGHCPAGLCCSRFNFCGSGPAYCGGAEEQVEAHP 412

Query: 64  STDVPQTRKVVVGAP 79
           STDVPQTRKVVVGAP
Sbjct: 413 STDVPQTRKVVVGAP 427

[Close]

BLAST of Spo28225.1 vs. NCBI nr
Match: gi|902212711|gb|KNA17066.1| (hypothetical protein SOVF_083390 [Spinacia oleracea])

HSP 1 Score: 151.8 bits (382), Expect = 5.100e-34
Identity = 72/78 (92.31%), Postives = 75/78 (96.15%), Query Frame = 1


		  
Query: 1  MMKVKSFVIIMVIMTLLVGTSMAQQGACNNGHCPAGLCCSRFNFCGSGPAYCGGAEEQVE 60
          MMKVKSFVIIMV+MTLLVGTSMAQQGACNNGHC AGLCCS+FNFCGSGPAYCGGAEEQV+
Sbjct: 1  MMKVKSFVIIMVVMTLLVGTSMAQQGACNNGHCAAGLCCSKFNFCGSGPAYCGGAEEQVQ 60

Query: 61 AHPSTDVPQTRKVVVGAP 79
          AHPSTDVPQTRKV  GAP
Sbjct: 61 AHPSTDVPQTRKVAAGAP 78

[Close]

BLAST of Spo28225.1 vs. NCBI nr
Match: gi|902208147|gb|KNA15864.1| (hypothetical protein SOVF_094440 [Spinacia oleracea])

HSP 1 Score: 84.3 bits (207), Expect = 1.000e-13
Identity = 43/72 (59.72%), Postives = 56/72 (77.78%), Query Frame = 1


		  
Query: 4  VKSFVIIMVIMTLLVGTSMAQQGACNN-GHCPAGLCCSRFNFCGSGPAYCGGAEEQVEAH 63
          +K FVI+MV+M+++VGTS+A  G CN  G CPAG CCS++ +CG GPAYCG AE+Q  AH
Sbjct: 2  MKMFVIVMVMMSMMVGTSIAS-GECNMYGRCPAGYCCSKYGYCGLGPAYCGDAEQQEAAH 61

Query: 64 PSTD-VPQTRKV 74
           + + VPQTRKV
Sbjct: 62 ATINVVPQTRKV 72

[Close]

BLAST of Spo28225.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RWM2_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_020890 PE=4 SV=1)

HSP 1 Score: 159.1 bits (401), Expect = 2.200e-36
Identity = 77/78 (98.72%), Postives = 77/78 (98.72%), Query Frame = 1


		  
Query: 1  MMKVKSFVIIMVIMTLLVGTSMAQQGACNNGHCPAGLCCSRFNFCGSGPAYCGGAEEQVE 60
          MMKVKSFVIIMVIMTLLVGTSMAQQGACNNGHCPAGLCCSRFNFCGSGPAYCGGAEEQVE
Sbjct: 1  MMKVKSFVIIMVIMTLLVGTSMAQQGACNNGHCPAGLCCSRFNFCGSGPAYCGGAEEQVE 60

Query: 61 AHPSTDVPQTRKVVVGAP 79
          AHPSTDVPQTRKVV GAP
Sbjct: 61 AHPSTDVPQTRKVVAGAP 78

[Close]

BLAST of Spo28225.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QRN5_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_156110 PE=4 SV=1)

HSP 1 Score: 159.1 bits (401), Expect = 2.200e-36
Identity = 77/78 (98.72%), Postives = 78/78 (100.00%), Query Frame = 1


		  
Query: 1  MMKVKSFVIIMVIMTLLVGTSMAQQGACNNGHCPAGLCCSRFNFCGSGPAYCGGAEEQVE 60
          M+KVKSFVIIMVIMTLLVGTSMAQQGACNNGHCPAGLCCSRFNFCGSGPAYCGGAEEQVE
Sbjct: 1  MIKVKSFVIIMVIMTLLVGTSMAQQGACNNGHCPAGLCCSRFNFCGSGPAYCGGAEEQVE 60

Query: 61 AHPSTDVPQTRKVVVGAP 79
          AHPSTDVPQTRKVVVGAP
Sbjct: 61 AHPSTDVPQTRKVVVGAP 78

[Close]

BLAST of Spo28225.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RUB6_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_033220 PE=4 SV=1)

HSP 1 Score: 156.0 bits (393), Expect = 1.900e-35
Identity = 75/75 (100.00%), Postives = 75/75 (100.00%), Query Frame = 1


		  
Query: 4   VKSFVIIMVIMTLLVGTSMAQQGACNNGHCPAGLCCSRFNFCGSGPAYCGGAEEQVEAHP 63
           VKSFVIIMVIMTLLVGTSMAQQGACNNGHCPAGLCCSRFNFCGSGPAYCGGAEEQVEAHP
Sbjct: 353 VKSFVIIMVIMTLLVGTSMAQQGACNNGHCPAGLCCSRFNFCGSGPAYCGGAEEQVEAHP 412

Query: 64  STDVPQTRKVVVGAP 79
           STDVPQTRKVVVGAP
Sbjct: 413 STDVPQTRKVVVGAP 427

[Close]

BLAST of Spo28225.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RC51_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_083390 PE=4 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 3.600e-34
Identity = 72/78 (92.31%), Postives = 75/78 (96.15%), Query Frame = 1


		  
Query: 1  MMKVKSFVIIMVIMTLLVGTSMAQQGACNNGHCPAGLCCSRFNFCGSGPAYCGGAEEQVE 60
          MMKVKSFVIIMV+MTLLVGTSMAQQGACNNGHC AGLCCS+FNFCGSGPAYCGGAEEQV+
Sbjct: 1  MMKVKSFVIIMVVMTLLVGTSMAQQGACNNGHCAAGLCCSKFNFCGSGPAYCGGAEEQVQ 60

Query: 61 AHPSTDVPQTRKVVVGAP 79
          AHPSTDVPQTRKV  GAP
Sbjct: 61 AHPSTDVPQTRKVAAGAP 78

[Close]

BLAST of Spo28225.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RA27_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_094440 PE=4 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 7.000e-14
Identity = 43/72 (59.72%), Postives = 56/72 (77.78%), Query Frame = 1


		  
Query: 4  VKSFVIIMVIMTLLVGTSMAQQGACNN-GHCPAGLCCSRFNFCGSGPAYCGGAEEQVEAH 63
          +K FVI+MV+M+++VGTS+A  G CN  G CPAG CCS++ +CG GPAYCG AE+Q  AH
Sbjct: 2  MKMFVIVMVMMSMMVGTSIAS-GECNMYGRCPAGYCCSKYGYCGLGPAYCGDAEQQEAAH 61

Query: 64 PSTD-VPQTRKV 74
           + + VPQTRKV
Sbjct: 62 ATINVVPQTRKV 72

[Close]

BLAST of Spo28225.1 vs. ExPASy Swiss-Prot
Match: AMP_AMACA (Antimicrobial peptide 2 OS=Amaranthus caudatus PE=1 SV=2)

HSP 1 Score: 57.4 bits (137), Expect = 8.200e-8
Identity = 27/71 (38.03%), Postives = 41/71 (57.75%), Query Frame = 1


		  
Query: 2  MKVKSFVIIMVIMTLLVGTSMAQQGACNNGHCPAGLCCSRFNFCGSGPAYCGGAEEQVEA 61
          MK  + ++I+++  ++V  SM   G C  G CP+G+CCS+F +CG GP YCG A   V+ 
Sbjct: 4  MKCVALIVIVMMAFMMVDPSMGV-GECVRGRCPSGMCCSQFGYCGKGPKYCGRASTTVDH 63

Query: 62 HPSTDVPQTRK 73
                 +T K
Sbjct: 64 QADVAATKTAK 73

[Close]

BLAST of Spo28225.1 vs. ExPASy Swiss-Prot
Match: AMP_AMARE (Antimicrobial peptide Ar-AMP OS=Amaranthus retroflexus PE=1 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 2.400e-7
Identity = 27/62 (43.55%), Postives = 40/62 (64.52%), Query Frame = 1


		  
Query: 1  MMKVKSFVIIMVIMT--LLVGTSMAQQGACNNGHCPAGLCCSRFNFCGSGPAYCGGAEEQ 60
          M+ +KS  +I+++M   ++V  SM   G C  G CP+G+CCS+F +CG GP YCG A   
Sbjct: 1  MVNMKSVALIVIVMMAFMMVDPSMGA-GECVQGRCPSGMCCSQFGYCGRGPKYCGRASTT 60

The following BLAST results are available for this feature:

BLAST of Spo28225.1 vs. NCBI nr
BLAST of Spo28225.1 vs. UniProtKB/TrEMBL
BLAST of Spo28225.1 vs. ExPASy Swiss-Prot
BLAST of Spo28225.1 vs. TAIR (Arabidopsis)

BLAST of Spo28225.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902178481\|gb\|KNA09156.1\|	3.2e-36	98.7	hypothetical protein SOVF_1561...	[more]
gi\|902235484\|gb\|KNA23935.1\|	3.2e-36	98.7	hypothetical protein SOVF_0208...	[more]
gi\|902232157\|gb\|KNA22524.1\|	2.7e-35	100.	hypothetical protein SOVF_0332...	[more]
gi\|902212711\|gb\|KNA17066.1\|	5.1e-34	92.3	hypothetical protein SOVF_0833...	[more]
gi\|902208147\|gb\|KNA15864.1\|	1.0e-13	59.7	hypothetical protein SOVF_0944...	[more]

BLAST of Spo28225.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9RWM2_SPIOL	2.2e-36	98.7	Uncharacterized protein OS=Spi...	[more]
A0A0K9QRN5_SPIOL	2.2e-36	98.7	Uncharacterized protein OS=Spi...	[more]
A0A0K9RUB6_SPIOL	1.9e-35	100.	Uncharacterized protein OS=Spi...	[more]
A0A0K9RC51_SPIOL	3.6e-34	92.3	Uncharacterized protein OS=Spi...	[more]
A0A0K9RA27_SPIOL	7.0e-14	59.7	Uncharacterized protein OS=Spi...	[more]

BLAST of Spo28225.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 2


Match Name	E-value	Identity	Description
AMP_AMACA	8.2e-8	38.0	Antimicrobial peptide 2 OS=Ama...	[more]
AMP_AMARE	2.4e-7	43.5	Antimicrobial peptide Ar-AMP O...	[more]

BLAST of Spo28225.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 0


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001002	Chitin-binding, type 1	GENE3D	3.30.60.10		coord: 28..58 score: 9.
IPR001002	Chitin-binding, type 1	PFAM	PF00187	Chitin_bind_1	coord: 29..53 score: 6.
IPR001002	Chitin-binding, type 1	SMART	SM00270	ChitinBD_3	coord: 27..60 score: 1.
IPR001002	Chitin-binding, type 1	unknown	SSF57016	Plant lectins/antimicrobial peptides	coord: 28..54 score: 5.8

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0050832	defense response to fungus
biological_process	GO:0050830	defense response to Gram-positive bacterium
biological_process	GO:0005982	starch metabolic process
biological_process	GO:0005985	sucrose metabolic process
biological_process	GO:0008152	metabolic process
molecular_function	GO:0008061	chitin binding
molecular_function	GO:0042973	glucan endo-1,3-beta-D-glucosidase activity
molecular_function	GO:0005515	protein binding
molecular_function	GO:0016740	transferase activity

RNA-Seq Expression

Co-expression


Gene	r value	Expression
Spo28224	0.99	Barchart \| Table
Spo28227	0.95	Barchart \| Table
Spo02607	0.74	Barchart \| Table
Spo08170	0.72	Barchart \| Table
Spo10790	0.72	Barchart \| Table
Spo00732	0.68	Barchart \| Table
Spo15600	0.67	Barchart \| Table
Spo28474	0.66	Barchart \| Table