Spo28328 (gene) | SpinachBase

Overview


Name	Spo28328
Type	gene
Organism	Spinacia oleracea (Spinach)
Description	Kunitz trypsin inhibitor
Location	chr1 : 50570161 .. 50570847 (+)

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTAACCACCTTCTTCTTCTAGCTGCAACCATCCTCTTTCTCTCTCCTCCCACGGTTACAGCCGCGGACTCGGTAGTCCTAGACATGGATGGGAACCCAGTTGAAGCAGGCTCAAGATACTACGTCCAAACTTTGGGAAGCAGGGGAGCAAGCCGTGGTGGCTTAGCCGCTGAACCCCTCAGTGGTATTTGCCCTTTATACGTAGGTCAACACAGGAGCTGGACTAACCAAGGCAGGCCCATAAGTTTCTACCCAGCTGACCCTTCACAGAAAAACATCACACTAAGTTCAGACGTCAACATTGCTTTTGGTCTAAACGGCTTGTGCAGGAACCAAGGAGTGTGGCAGCTGACTTCAAATGGGCAAGGAGTATTCTATGTAACTACTAATGGAGAAATCGGAAACCCAGGTGCTTCCACCTTCACCAACTGGTTCAAGCTCGAAGAAGCGTTGCCTGGAACTAATTCATACAAAATAGTGTATTGTTTCCTTGTACCTGTTCCTGCTGGAAATGGTAAGAGTCATCCTGATTCTGAGTATTGTGAACAGTTGGATGCTACTCTACCTGGGCCTTGGGATTTGTCGTTGTTAAGTTTGGTACCAATTGATAATCCTCTTCCCTTCTTTGGTTTTGTATTCAAGAAGGTCACCAGTACTACTACTGCCACTAGTAGTAGCTACTAG

mRNA sequence

ATGGCTAACCACCTTCTTCTTCTAGCTGCAACCATCCTCTTTCTCTCTCCTCCCACGGTTACAGCCGCGGACTCGGTAGTCCTAGACATGGATGGGAACCCAGTTGAAGCAGGCTCAAGATACTACGTCCAAACTTTGGGAAGCAGGGGAGCAAGCCGTGGTGGCTTAGCCGCTGAACCCCTCAGTGGTATTTGCCCTTTATACGTAGGTCAACACAGGAGCTGGACTAACCAAGGCAGGCCCATAAGTTTCTACCCAGCTGACCCTTCACAGAAAAACATCACACTAAGTTCAGACGTCAACATTGCTTTTGGTCTAAACGGCTTGTGCAGGAACCAAGGAGTGTGGCAGCTGACTTCAAATGGGCAAGGAGTATTCTATGTAACTACTAATGGAGAAATCGGAAACCCAGGTGCTTCCACCTTCACCAACTGGTTCAAGCTCGAAGAAGCGTTGCCTGGAACTAATTCATACAAAATAGTGTATTGTTTCCTTGTACCTGTTCCTGCTGGAAATGGTAAGAGTCATCCTGATTCTGAGTATTGTGAACAGTTGGATGCTACTCTACCTGGGCCTTGGGATTTGTCGTTGTTAAGTTTGGTACCAATTGATAATCCTCTTCCCTTCTTTGGTTTTGTATTCAAGAAGGTCACCAGTACTACTACTGCCACTAGTAGTAGCTACTAG

Coding sequence (CDS)

ATGGCTAACCACCTTCTTCTTCTAGCTGCAACCATCCTCTTTCTCTCTCCTCCCACGGTTACAGCCGCGGACTCGGTAGTCCTAGACATGGATGGGAACCCAGTTGAAGCAGGCTCAAGATACTACGTCCAAACTTTGGGAAGCAGGGGAGCAAGCCGTGGTGGCTTAGCCGCTGAACCCCTCAGTGGTATTTGCCCTTTATACGTAGGTCAACACAGGAGCTGGACTAACCAAGGCAGGCCCATAAGTTTCTACCCAGCTGACCCTTCACAGAAAAACATCACACTAAGTTCAGACGTCAACATTGCTTTTGGTCTAAACGGCTTGTGCAGGAACCAAGGAGTGTGGCAGCTGACTTCAAATGGGCAAGGAGTATTCTATGTAACTACTAATGGAGAAATCGGAAACCCAGGTGCTTCCACCTTCACCAACTGGTTCAAGCTCGAAGAAGCGTTGCCTGGAACTAATTCATACAAAATAGTGTATTGTTTCCTTGTACCTGTTCCTGCTGGAAATGGTAAGAGTCATCCTGATTCTGAGTATTGTGAACAGTTGGATGCTACTCTACCTGGGCCTTGGGATTTGTCGTTGTTAAGTTTGGTACCAATTGATAATCCTCTTCCCTTCTTTGGTTTTGTATTCAAGAAGGTCACCAGTACTACTACTGCCACTAGTAGTAGCTACTAG

Protein sequence

MANHLLLLAATILFLSPPTVTAADSVVLDMDGNPVEAGSRYYVQTLGSRGASRGGLAAEPLSGICPLYVGQHRSWTNQGRPISFYPADPSQKNITLSSDVNIAFGLNGLCRNQGVWQLTSNGQGVFYVTTNGEIGNPGASTFTNWFKLEEALPGTNSYKIVYCFLVPVPAGNGKSHPDSEYCEQLDATLPGPWDLSLLSLVPIDNPLPFFGFVFKKVTSTTTATSSSY

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spo28328.1	Spo28328.1	mRNA

Homology

[Close]

BLAST of Spo28328.1 vs. NCBI nr
Match: gi|902078036|gb|KNA03096.1| (hypothetical protein SOVF_212410 [Spinacia oleracea])

HSP 1 Score: 411.8 bits (1057), Expect = 8.000e-112
Identity = 197/200 (98.50%), Postives = 198/200 (99.00%), Query Frame = 1


		  
Query: 1   MANHLLLLAATILFLSPPTVTAADSVVLDMDGNPVEAGSRYYVQTLGSRGASRGGLAAEP 60
           MANHLLLLAATILFLSPPTVTAADSVVLDMDGNPVEAGSRYYVQTLGSRGASRGGLAAEP
Sbjct: 1   MANHLLLLAATILFLSPPTVTAADSVVLDMDGNPVEAGSRYYVQTLGSRGASRGGLAAEP 60

Query: 61  LSGICPLYVGQHRSWTNQGRPISFYPADPSQKNITLSSDVNIAFGLNGLCRNQGVWQLTS 120
           LSGICPLYVGQHRSWTNQGRPI+FYPADPSQKNITLSSDVNIAFGLNGLCRNQGVWQLTS
Sbjct: 61  LSGICPLYVGQHRSWTNQGRPITFYPADPSQKNITLSSDVNIAFGLNGLCRNQGVWQLTS 120

Query: 121 NGQGVFYVTTNGEIGNPGASTFTNWFKLEEALPGTNSYKIVYCFLVPVPAGNGKSHPDSE 180
           NG GVFYVTTNGEIGNPGA TFTNWFKLEEALPGTNSYKIVYCFLVPVPAGNGKSHPDSE
Sbjct: 121 NGDGVFYVTTNGEIGNPGAETFTNWFKLEEALPGTNSYKIVYCFLVPVPAGNGKSHPDSE 180

Query: 181 YCEQLDATLPGPWDLSLLSL 201
           YCEQLDATLPGPWDLSLLSL
Sbjct: 181 YCEQLDATLPGPWDLSLLSL 200

[Close]

BLAST of Spo28328.1 vs. NCBI nr
Match: gi|902188089|gb|KNA11068.1| (hypothetical protein SOVF_138590 [Spinacia oleracea])

HSP 1 Score: 347.8 bits (891), Expect = 1.400e-92
Identity = 179/238 (75.21%), Postives = 196/238 (82.35%), Query Frame = 1


		  
Query: 1   MANHLLLLAATI----LFLSPPTVTAADSVVLDMDGNPVEAGSRYYVQTLGSRGASRGGL 60
           MANHLLL AATI    LFLSPPTVTAADSVVLDMDGNP+EAGS+YYV TLG+RGA RGGL
Sbjct: 1   MANHLLLPAATIILTLLFLSPPTVTAADSVVLDMDGNPLEAGSQYYVLTLGTRGAGRGGL 60

Query: 61  AAEPLSGICPLYVGQHRSWTNQGRPISFYPADPSQKNITLSSDVNIAFGLNGLCRNQGVW 120
            AE L+G CPLYVGQHRSWT QG P++FYPAD SQKNIT SSDVNIAF  + +CRNQGVW
Sbjct: 61  TAERLTGPCPLYVGQHRSWTQQGSPVTFYPADSSQKNITQSSDVNIAFNPSPICRNQGVW 120

Query: 121 QLTSNGQGVFYVTTNGEIGNPGASTFTNWFKLEEALPGTNSYKIVYCFLVP------VPA 180
           QL+SNG GVF+VTTNG IGNPGA TFTNWFK+E+A    +SYKIVYCF+VP       P 
Sbjct: 121 QLSSNGLGVFHVTTNGVIGNPGAETFTNWFKIEKASSEPDSYKIVYCFVVPGPGPVPAPP 180

Query: 181 GNGKSHPDSEYCEQLDATLP-GPWDLSLLSLVPIDNPLPFFGFVFKKVTSTTTATSSS 228
             GK+ PD EYCEQLDAT+P  P D SLLSLVPIDNPLPFFGFVFKKVT+TTT  SSS
Sbjct: 181 ETGKNRPD-EYCEQLDATIPVPPLDFSLLSLVPIDNPLPFFGFVFKKVTTTTTTISSS 237

[Close]

BLAST of Spo28328.1 vs. NCBI nr
Match: gi|902228866|gb|KNA21297.1| (hypothetical protein SOVF_044560 [Spinacia oleracea])

HSP 1 Score: 347.8 bits (891), Expect = 1.400e-92
Identity = 177/246 (71.95%), Postives = 200/246 (81.30%), Query Frame = 1


		  
Query: 1   MANHLLLLAATI----LFLSPPTVTA--ADSVVLDMDGNPVEAGSRYYVQTLGSRGASRG 60
           MANHLLL AATI    LFLSP T TA  ADSVV+D+DGNP+EAGS+YYV T GSRGA RG
Sbjct: 1   MANHLLLPAATIFLTLLFLSPATATATAADSVVVDIDGNPLEAGSQYYVLTSGSRGAGRG 60

Query: 61  GLAAEPLSGICPLYVGQHRSWTNQGRPISFYPADPSQKNITLSSDVNIAFGLNGLCRNQG 120
           GL A PL GICPLYVGQHRSW N+G P+SFYPAD SQKNITLSSDVNIAFGL+ +CRNQG
Sbjct: 61  GLNAVPLGGICPLYVGQHRSWNNRGSPVSFYPADLSQKNITLSSDVNIAFGLSAMCRNQG 120

Query: 121 VWQLTSNGQGVFYVTTNGEIGNPGASTFTNWFKLEE-ALPGTNSYKIVYCFLVPVPA--- 180
           VWQL+SN  GVFY+TTNGEIGNPGA TFTNWFK+E+   P +NSY+IVYCF VP P    
Sbjct: 121 VWQLSSNELGVFYITTNGEIGNPGAETFTNWFKIEKLPFPKSNSYRIVYCFAVPKPGPEP 180

Query: 181 -----GNGKSHPDS----EYCEQLDATLPGPWDLSLLSLVPIDNPLPFFGFVFKKVTSTT 228
                 + ++ P+S    EYC+QLDATLPGPWDLS+LSLVPIDNPLPFFGFVFKKVT+TT
Sbjct: 181 VHVVPDSRRTGPESVVPTEYCQQLDATLPGPWDLSMLSLVPIDNPLPFFGFVFKKVTTTT 240

[Close]

BLAST of Spo28328.1 vs. NCBI nr
Match: gi|902188088|gb|KNA11067.1| (hypothetical protein SOVF_138580 [Spinacia oleracea])

HSP 1 Score: 322.4 bits (825), Expect = 6.400e-85
Identity = 166/224 (74.11%), Postives = 182/224 (81.25%), Query Frame = 1


		  
Query: 1   MANHLLLLAATIL----FLSPPTVTAADSVVLDMDGNPVEAGSRYYVQTLGSRGASRGGL 60
           MA+HLLL AATIL    FLSPPT  AADSVVLD+DGNPVEAGS+YYV T G+RGA RGGL
Sbjct: 1   MAHHLLLAAATILLTLFFLSPPT--AADSVVLDIDGNPVEAGSQYYVLTRGTRGAGRGGL 60

Query: 61  AAEPLSGICPLYVGQHRSWTNQGRPISFYPADPSQKNITLSSDVNIAFGLNGLCRNQGVW 120
            A PL G+CPLYVGQHRSWT QG P++F+PA+PSQK IT SSDVNIAFGLN +CRN GVW
Sbjct: 61  TAAPLGGLCPLYVGQHRSWTQQGSPVTFFPANPSQKYITQSSDVNIAFGLNPICRNLGVW 120

Query: 121 QLTSNGQGVFYVTTNGEIGNPGASTFTNWFKLEEALPGTNSYKIVYCFLVPVPAGNGKSH 180
           QL  +  GV YVTTNG IGNPGA TFTNWFK+E A  G  SYKIVYCFLVP P  +  S 
Sbjct: 121 QLGYSFSGVPYVTTNGVIGNPGAETFTNWFKIEHAF-GPESYKIVYCFLVPAPWYSRMS- 180

Query: 181 PDSEYCEQLDATLPGPWDLSLLSLVPIDNPLPFFGFVFKKVTST 221
              EYCEQLDAT+PGP DL++LSLVPID PLPFFGFVFKKVTST
Sbjct: 181 -PYEYCEQLDATIPGPSDLAMLSLVPIDKPLPFFGFVFKKVTST 219

[Close]

BLAST of Spo28328.1 vs. NCBI nr
Match: gi|902228865|gb|KNA21296.1| (hypothetical protein SOVF_044550 [Spinacia oleracea])

HSP 1 Score: 277.3 bits (708), Expect = 2.400e-71
Identity = 152/237 (64.14%), Postives = 174/237 (73.42%), Query Frame = 1


		  
Query: 1   MANHLLLLAATIL----FLSPPTVTAADSVVLDMDGNPVEAGSRYYVQTLGSRGASRGGL 60
           MA+HLLL AAT+L    FLSPPT TA DSVVLD+DGNPVEAGS+YYV T G  GA RGGL
Sbjct: 1   MAHHLLLPAATLLLTLLFLSPPTATAKDSVVLDVDGNPVEAGSQYYVLTSGWGGAGRGGL 60

Query: 61  AAEPLSGICPLYVGQHRSWTNQGRPISFYPADPSQKNITLSSDVNIAFGLNGLCRNQGVW 120
            A  L G CPLYVGQ+R W  +G P++FYP++PSQK+IT SSDVNIAF  N +CRNQGVW
Sbjct: 61  TAASLGGPCPLYVGQYRLWVQKGSPVTFYPSNPSQKHITQSSDVNIAFAPNPICRNQGVW 120

Query: 121 QLTSNGQGVFYVTTNGEIG-NPGASTFTNWFKLEEA--LPGTNSYKIVYCFLVP------ 180
           QL  NG   FY+TTNG IG      TF NWFK+E+A  LPG  +YKIVYCF VP      
Sbjct: 121 QLGYNGIS-FYITTNGVIGKQEDVDTFANWFKIEQAGGLPG--AYKIVYCFAVPSTGPEP 180

Query: 181 -VPAGNGKS--HPDSEYCEQLDATLP-GPWDLSLLSLVPIDNPLPFFGFVFKKVTST 221
            VP    ++   PD+EYCEQLDAT+P  P  LSLL+ VPIDNPLPFFGFVFKKV +T
Sbjct: 181 VVPDSRRRTGPEPDTEYCEQLDATVPVPPMGLSLLTPVPIDNPLPFFGFVFKKVNTT 234

[Close]

BLAST of Spo28328.1 vs. UniProtKB/TrEMBL
Match: A0A0K9Q9A5_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_212410 PE=4 SV=1)

HSP 1 Score: 411.8 bits (1057), Expect = 5.600e-112
Identity = 197/200 (98.50%), Postives = 198/200 (99.00%), Query Frame = 1


		  
Query: 1   MANHLLLLAATILFLSPPTVTAADSVVLDMDGNPVEAGSRYYVQTLGSRGASRGGLAAEP 60
           MANHLLLLAATILFLSPPTVTAADSVVLDMDGNPVEAGSRYYVQTLGSRGASRGGLAAEP
Sbjct: 1   MANHLLLLAATILFLSPPTVTAADSVVLDMDGNPVEAGSRYYVQTLGSRGASRGGLAAEP 60

Query: 61  LSGICPLYVGQHRSWTNQGRPISFYPADPSQKNITLSSDVNIAFGLNGLCRNQGVWQLTS 120
           LSGICPLYVGQHRSWTNQGRPI+FYPADPSQKNITLSSDVNIAFGLNGLCRNQGVWQLTS
Sbjct: 61  LSGICPLYVGQHRSWTNQGRPITFYPADPSQKNITLSSDVNIAFGLNGLCRNQGVWQLTS 120

Query: 121 NGQGVFYVTTNGEIGNPGASTFTNWFKLEEALPGTNSYKIVYCFLVPVPAGNGKSHPDSE 180
           NG GVFYVTTNGEIGNPGA TFTNWFKLEEALPGTNSYKIVYCFLVPVPAGNGKSHPDSE
Sbjct: 121 NGDGVFYVTTNGEIGNPGAETFTNWFKLEEALPGTNSYKIVYCFLVPVPAGNGKSHPDSE 180

Query: 181 YCEQLDATLPGPWDLSLLSL 201
           YCEQLDATLPGPWDLSLLSL
Sbjct: 181 YCEQLDATLPGPWDLSLLSL 200

[Close]

BLAST of Spo28328.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QWG7_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_138590 PE=4 SV=1)

HSP 1 Score: 347.8 bits (891), Expect = 9.900e-93
Identity = 179/238 (75.21%), Postives = 196/238 (82.35%), Query Frame = 1


		  
Query: 1   MANHLLLLAATI----LFLSPPTVTAADSVVLDMDGNPVEAGSRYYVQTLGSRGASRGGL 60
           MANHLLL AATI    LFLSPPTVTAADSVVLDMDGNP+EAGS+YYV TLG+RGA RGGL
Sbjct: 1   MANHLLLPAATIILTLLFLSPPTVTAADSVVLDMDGNPLEAGSQYYVLTLGTRGAGRGGL 60

Query: 61  AAEPLSGICPLYVGQHRSWTNQGRPISFYPADPSQKNITLSSDVNIAFGLNGLCRNQGVW 120
            AE L+G CPLYVGQHRSWT QG P++FYPAD SQKNIT SSDVNIAF  + +CRNQGVW
Sbjct: 61  TAERLTGPCPLYVGQHRSWTQQGSPVTFYPADSSQKNITQSSDVNIAFNPSPICRNQGVW 120

Query: 121 QLTSNGQGVFYVTTNGEIGNPGASTFTNWFKLEEALPGTNSYKIVYCFLVP------VPA 180
           QL+SNG GVF+VTTNG IGNPGA TFTNWFK+E+A    +SYKIVYCF+VP       P 
Sbjct: 121 QLSSNGLGVFHVTTNGVIGNPGAETFTNWFKIEKASSEPDSYKIVYCFVVPGPGPVPAPP 180

Query: 181 GNGKSHPDSEYCEQLDATLP-GPWDLSLLSLVPIDNPLPFFGFVFKKVTSTTTATSSS 228
             GK+ PD EYCEQLDAT+P  P D SLLSLVPIDNPLPFFGFVFKKVT+TTT  SSS
Sbjct: 181 ETGKNRPD-EYCEQLDATIPVPPLDFSLLSLVPIDNPLPFFGFVFKKVTTTTTTISSS 237

[Close]

BLAST of Spo28328.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RR37_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_044560 PE=4 SV=1)

HSP 1 Score: 347.8 bits (891), Expect = 9.900e-93
Identity = 177/246 (71.95%), Postives = 200/246 (81.30%), Query Frame = 1


		  
Query: 1   MANHLLLLAATI----LFLSPPTVTA--ADSVVLDMDGNPVEAGSRYYVQTLGSRGASRG 60
           MANHLLL AATI    LFLSP T TA  ADSVV+D+DGNP+EAGS+YYV T GSRGA RG
Sbjct: 1   MANHLLLPAATIFLTLLFLSPATATATAADSVVVDIDGNPLEAGSQYYVLTSGSRGAGRG 60

Query: 61  GLAAEPLSGICPLYVGQHRSWTNQGRPISFYPADPSQKNITLSSDVNIAFGLNGLCRNQG 120
           GL A PL GICPLYVGQHRSW N+G P+SFYPAD SQKNITLSSDVNIAFGL+ +CRNQG
Sbjct: 61  GLNAVPLGGICPLYVGQHRSWNNRGSPVSFYPADLSQKNITLSSDVNIAFGLSAMCRNQG 120

Query: 121 VWQLTSNGQGVFYVTTNGEIGNPGASTFTNWFKLEE-ALPGTNSYKIVYCFLVPVPA--- 180
           VWQL+SN  GVFY+TTNGEIGNPGA TFTNWFK+E+   P +NSY+IVYCF VP P    
Sbjct: 121 VWQLSSNELGVFYITTNGEIGNPGAETFTNWFKIEKLPFPKSNSYRIVYCFAVPKPGPEP 180

Query: 181 -----GNGKSHPDS----EYCEQLDATLPGPWDLSLLSLVPIDNPLPFFGFVFKKVTSTT 228
                 + ++ P+S    EYC+QLDATLPGPWDLS+LSLVPIDNPLPFFGFVFKKVT+TT
Sbjct: 181 VHVVPDSRRTGPESVVPTEYCQQLDATLPGPWDLSMLSLVPIDNPLPFFGFVFKKVTTTT 240

[Close]

BLAST of Spo28328.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QUV4_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_138580 PE=4 SV=1)

HSP 1 Score: 322.4 bits (825), Expect = 4.400e-85
Identity = 166/224 (74.11%), Postives = 182/224 (81.25%), Query Frame = 1


		  
Query: 1   MANHLLLLAATIL----FLSPPTVTAADSVVLDMDGNPVEAGSRYYVQTLGSRGASRGGL 60
           MA+HLLL AATIL    FLSPPT  AADSVVLD+DGNPVEAGS+YYV T G+RGA RGGL
Sbjct: 1   MAHHLLLAAATILLTLFFLSPPT--AADSVVLDIDGNPVEAGSQYYVLTRGTRGAGRGGL 60

Query: 61  AAEPLSGICPLYVGQHRSWTNQGRPISFYPADPSQKNITLSSDVNIAFGLNGLCRNQGVW 120
            A PL G+CPLYVGQHRSWT QG P++F+PA+PSQK IT SSDVNIAFGLN +CRN GVW
Sbjct: 61  TAAPLGGLCPLYVGQHRSWTQQGSPVTFFPANPSQKYITQSSDVNIAFGLNPICRNLGVW 120

Query: 121 QLTSNGQGVFYVTTNGEIGNPGASTFTNWFKLEEALPGTNSYKIVYCFLVPVPAGNGKSH 180
           QL  +  GV YVTTNG IGNPGA TFTNWFK+E A  G  SYKIVYCFLVP P  +  S 
Sbjct: 121 QLGYSFSGVPYVTTNGVIGNPGAETFTNWFKIEHAF-GPESYKIVYCFLVPAPWYSRMS- 180

Query: 181 PDSEYCEQLDATLPGPWDLSLLSLVPIDNPLPFFGFVFKKVTST 221
              EYCEQLDAT+PGP DL++LSLVPID PLPFFGFVFKKVTST
Sbjct: 181 -PYEYCEQLDATIPGPSDLAMLSLVPIDKPLPFFGFVFKKVTST 219

[Close]

BLAST of Spo28328.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RP34_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_044550 PE=4 SV=1)

HSP 1 Score: 277.3 bits (708), Expect = 1.600e-71
Identity = 152/237 (64.14%), Postives = 174/237 (73.42%), Query Frame = 1


		  
Query: 1   MANHLLLLAATIL----FLSPPTVTAADSVVLDMDGNPVEAGSRYYVQTLGSRGASRGGL 60
           MA+HLLL AAT+L    FLSPPT TA DSVVLD+DGNPVEAGS+YYV T G  GA RGGL
Sbjct: 1   MAHHLLLPAATLLLTLLFLSPPTATAKDSVVLDVDGNPVEAGSQYYVLTSGWGGAGRGGL 60

Query: 61  AAEPLSGICPLYVGQHRSWTNQGRPISFYPADPSQKNITLSSDVNIAFGLNGLCRNQGVW 120
            A  L G CPLYVGQ+R W  +G P++FYP++PSQK+IT SSDVNIAF  N +CRNQGVW
Sbjct: 61  TAASLGGPCPLYVGQYRLWVQKGSPVTFYPSNPSQKHITQSSDVNIAFAPNPICRNQGVW 120

Query: 121 QLTSNGQGVFYVTTNGEIG-NPGASTFTNWFKLEEA--LPGTNSYKIVYCFLVP------ 180
           QL  NG   FY+TTNG IG      TF NWFK+E+A  LPG  +YKIVYCF VP      
Sbjct: 121 QLGYNGIS-FYITTNGVIGKQEDVDTFANWFKIEQAGGLPG--AYKIVYCFAVPSTGPEP 180

Query: 181 -VPAGNGKS--HPDSEYCEQLDATLP-GPWDLSLLSLVPIDNPLPFFGFVFKKVTST 221
            VP    ++   PD+EYCEQLDAT+P  P  LSLL+ VPIDNPLPFFGFVFKKV +T
Sbjct: 181 VVPDSRRRTGPEPDTEYCEQLDATVPVPPMGLSLLTPVPIDNPLPFFGFVFKKVNTT 234

[Close]

BLAST of Spo28328.1 vs. ExPASy Swiss-Prot
Match: ASP_THECC (21 kDa seed protein OS=Theobroma cacao GN=ASP PE=2 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 1.400e-18
Identity = 66/164 (40.24%), Postives = 89/164 (54.27%), Query Frame = 1


		  
Query: 5   LLLLAATILFLSPPTVTAADSVVLDMDGNPVEAGSRYYVQTLGSRGASRGGLAAEPLSG- 64
           LLL A T          AA+S VLD DG+ ++ G +YYV +  S GA  GGLA    +G 
Sbjct: 9   LLLFAFTSKSYFFGVANAANSPVLDTDGDELQTGVQYYVLSSIS-GAGGGGLALGRATGQ 68

Query: 65  ICPLYVGQHRSWTNQGRPISFYPADPSQKNITLSSDVNIAFG--LNGLCRNQGVWQLTS- 124
            CP  V Q RS  + G P+ F  AD     + +S+DVNI F    + LC    VW+L + 
Sbjct: 69  SCPEIVVQRRSDLDNGTPVIFSNADSKDDVVRVSTDVNIEFVPIRDRLCSTSTVWRLDNY 128

Query: 125 -NGQGVFYVTTNGEIGNPGASTFTNWFKLEEALPGTNSYKIVYC 164
            N  G ++VTT+G  G PG +T  +WFK+E+A  G   YK  +C
Sbjct: 129 DNSAGKWWVTTDGVKGEPGPNTLCSWFKIEKA--GVLGYKFRFC 169

[Close]

BLAST of Spo28328.1 vs. ExPASy Swiss-Prot
Match: MIRA_SYNDU (Miraculin OS=Synsepalum dulcificum PE=1 SV=3)

HSP 1 Score: 92.8 bits (229), Expect = 5.100e-18
Identity = 58/165 (35.15%), Postives = 88/165 (53.33%), Query Frame = 1


		  
Query: 8   LAATILFLSPPTVTAADSV---VLDMDGNPVEAGSRYY-VQTLGSRGASRGGLAAEPLSG 67
           ++A +   + P ++AADS    VLD+DG  +  G+ YY V  L   G      A  P   
Sbjct: 14  VSALLAAAANPLLSAADSAPNPVLDIDGEKLRTGTNYYIVPVLRDHGGGLTVSATTPNGT 73

Query: 68  -ICPLYVGQHRSWTNQGRPISFYPADPSQKNITLSSDVNIAFGLNGLCR--NQGVWQLT- 127
            +CP  V Q R   +  RP++F+P +P +  + +S+D+NI F     CR  +  VW+L  
Sbjct: 74  FVCPPRVVQTRKEVDHDRPLAFFPENPKEDVVRVSTDLNINFSAFMPCRWTSSTVWRLDK 133

Query: 128 -SNGQGVFYVTTNGEIGNPGASTFTNWFKLEEALPGTNSYKIVYC 164
                G ++VT  G  GNPG  T ++WFK+EE   G+  YK+V+C
Sbjct: 134 YDESTGQYFVTIGGVKGNPGPETISSWFKIEE-FCGSGFYKLVFC 177

[Close]

BLAST of Spo28328.1 vs. ExPASy Swiss-Prot
Match: IAAS_HORVU (Alpha-amylase/subtilisin inhibitor OS=Hordeum vulgare PE=1 SV=2)

HSP 1 Score: 65.9 bits (159), Expect = 6.700e-10
Identity = 51/160 (31.87%), Postives = 78/160 (48.75%), Query Frame = 1


		  
Query: 9   AATILFLSPPTVTAADSVVLDMDGNPVEAGSRYYVQTLGSRGASRGGLAAEPLSGI-CPL 68
           ++ + + +PP+  A    V D DG+ + A + YYV  L +  A  GGL   P  G  CPL
Sbjct: 10  SSPLFWPAPPSRAADPPPVHDTDGHELRADANYYV--LSANRAHGGGLTMAPGHGRHCPL 69

Query: 69  YVGQHRSWTNQGRPISFYP--ADPSQKNITLSSDVNIAFGLNGLCRNQGVWQLTSN-GQG 128
           +V Q  +  + G P+   P    PS K I LS+DV I+F     C     W + S    G
Sbjct: 70  FVSQDPNGQHDGFPVRITPYGVAPSDKIIRLSTDVRISFRAYTTCLQSTEWHIDSELAAG 129

Query: 129 VFYVTTNGEIGNPGASTFTNWFKLEE-ALPGTNSYKIVYC 164
             +V T G + +P  S   N F++E+ +    + YK++ C
Sbjct: 130 RRHVIT-GPVKDPSPSGRENAFRIEKYSGAEVHEYKLMSC 166

[Close]

BLAST of Spo28328.1 vs. ExPASy Swiss-Prot
Match: IAAS_WHEAT (Endogenous alpha-amylase/subtilisin inhibitor OS=Triticum aestivum PE=1 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 4.400e-9
Identity = 49/142 (34.51%), Postives = 67/142 (47.18%), Query Frame = 1


		  
Query: 27  VLDMDGNPVEAGSRYYVQTLGSRGASRGGLAAEPLSGI-CPLYVGQHRSWTNQGRPISFY 86
           V D DGN + A + YYV  L +  A  GGL   P  G  CPL+V Q       G P+   
Sbjct: 5   VHDTDGNELRADANYYV--LPANRAHGGGLTMAPGHGRRCPLFVSQEADGQRDGLPVRIA 64

Query: 87  P--ADPSQKNITLSSDVNIAFGLNGLCRNQGVWQLTSN-GQGVFYVTTNGEIGNPGASTF 146
           P    PS K I LS+DV I+F     C     W + S    G  +V T G + +P  S  
Sbjct: 65  PHGGAPSDKIIRLSTDVRISFRAYTTCVQSTEWHIDSELVSGRRHVIT-GPVRDPSPSGR 124

Query: 147 TNWFKLEE-ALPGTNSYKIVYC 164
            N F++E+ +    + YK++ C
Sbjct: 125 ENAFRIEKYSGAEVHEYKLMAC 143

The following BLAST results are available for this feature:

BLAST of Spo28328.1 vs. NCBI nr
BLAST of Spo28328.1 vs. UniProtKB/TrEMBL
BLAST of Spo28328.1 vs. ExPASy Swiss-Prot
BLAST of Spo28328.1 vs. TAIR (Arabidopsis)

BLAST of Spo28328.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902078036\|gb\|KNA03096.1\|	8.0e-112	98.5	hypothetical protein SOVF_2124...	[more]
gi\|902188089\|gb\|KNA11068.1\|	1.4e-92	75.2	hypothetical protein SOVF_1385...	[more]
gi\|902228866\|gb\|KNA21297.1\|	1.4e-92	71.9	hypothetical protein SOVF_0445...	[more]
gi\|902188088\|gb\|KNA11067.1\|	6.4e-85	74.1	hypothetical protein SOVF_1385...	[more]
gi\|902228865\|gb\|KNA21296.1\|	2.4e-71	64.1	hypothetical protein SOVF_0445...	[more]

BLAST of Spo28328.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9Q9A5_SPIOL	5.6e-112	98.5	Uncharacterized protein OS=Spi...	[more]
A0A0K9QWG7_SPIOL	9.9e-93	75.2	Uncharacterized protein OS=Spi...	[more]
A0A0K9RR37_SPIOL	9.9e-93	71.9	Uncharacterized protein OS=Spi...	[more]
A0A0K9QUV4_SPIOL	4.4e-85	74.1	Uncharacterized protein OS=Spi...	[more]
A0A0K9RP34_SPIOL	1.6e-71	64.1	Uncharacterized protein OS=Spi...	[more]

BLAST of Spo28328.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 4


Match Name	E-value	Identity	Description
ASP_THECC	1.4e-18	40.2	21 kDa seed protein OS=Theobro...	[more]
MIRA_SYNDU	5.1e-18	35.1	Miraculin OS=Synsepalum dulcif...	[more]
IAAS_HORVU	6.7e-10	31.8	Alpha-amylase/subtilisin inhib...	[more]
IAAS_WHEAT	4.4e-9	34.5	Endogenous alpha-amylase/subti...	[more]

BLAST of Spo28328.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 0


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002160	Proteinase inhibitor I3, Kunitz legume	PRINTS	PR00291	KUNITZINHBTR	coord: 65..85 score: 4.4E-6coord: 145..164 score: 4.4E-6coord: 26..55 score: 4.
IPR002160	Proteinase inhibitor I3, Kunitz legume	PFAM	PF00197	Kunitz_legume	coord: 27..165 score: 4.6
IPR002160	Proteinase inhibitor I3, Kunitz legume	SMART	SM00452	kul_2	coord: 26..218 score: 4.0
IPR002160	Proteinase inhibitor I3, Kunitz legume	PROSITE	PS00283	SOYBEAN_KUNITZ	coord: 27..43 scor
IPR011065	Kunitz inhibitor ST1-like	unknown	SSF50386	STI-like	coord: 23..166 score: 4.86
None	No IPR available	GENE3D	2.80.10.50		coord: 23..165 score: 1.3
None	No IPR available	PANTHER	PTHR33107	FAMILY NOT NAMED	coord: 5..163 score: 1.9
None	No IPR available	PANTHER	PTHR33107:SF5	KUNITZ FAMILY TRYPSIN AND PROTEASE INHIBITOR PROTEIN-RELATED	coord: 5..163 score: 1.9

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0008152	metabolic process
cellular_component	GO:0009507	chloroplast
molecular_function	GO:0004866	endopeptidase inhibitor activity
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016706	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors
molecular_function	GO:0046423	allene-oxide cyclase activity

RNA-Seq Expression

Co-expression


Gene	r value	Expression
Spo28095	0.74	Barchart \| Table
Spo24067	0.69	Barchart \| Table
Spo11168	0.68	Barchart \| Table
Spo07283	0.66	Barchart \| Table