Spo28443 (gene) | SpinachBase

Overview


Name	Spo28443
Type	gene
Organism	Spinacia oleracea (Spinach)
Description	Kunitz trypsin inhibitor
Location	SpoScf_05128 : 6183 .. 6623 (+)

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCCTCAGCCCAATTAGCCGAAGGCTGGAAAGGGGAATCCCCAATAATGTTCCTCCCATCTGACCCATCAAACAAACATATCCACATTTCTTCAGACCTTAACATAATATTCAACAACACCACATCTACCGTCTGCTCTCAAGGGGCATGGCAGCTCACCCCCGATGCTAACTCCGGTAAATTATACTTATCTACCGGAGGAAGTATCGGAAAACCGAGTTCGCAAACGACAGCTGCTAATTGGTTAAAGATAGAGAGAAGTAGTGTTGATTCGAGCTTTTATCAACTCGAGTATTGTCCTAGTAGTACTTCAACTAATATAGTGTGCGGCGTTATCGACGCTCTTGATTATACAGATGATTTCCGCTTGGTGTGGTTGGGTCTCTCACCGGTTCGTATCACTAATTTCTTCCATTTGCTCAGTTTCATTAAAGCTTAA

mRNA sequence

ATGCCCTCAGCCCAATTAGCCGAAGGCTGGAAAGGGGAATCCCCAATAATGTTCCTCCCATCTGACCCATCAAACAAACATATCCACATTTCTTCAGACCTTAACATAATATTCAACAACACCACATCTACCGTCTGCTCTCAAGGGGCATGGCAGCTCACCCCCGATGCTAACTCCGGTAAATTATACTTATCTACCGGAGGAAGTATCGGAAAACCGAGTTCGCAAACGACAGCTGCTAATTGGTTAAAGATAGAGAGAAGTAGTGTTGATTCGAGCTTTTATCAACTCGAGTATTGTCCTAGTAGTACTTCAACTAATATAGTGTGCGGCGTTATCGACGCTCTTGATTATACAGATGATTTCCGCTTGGTGTGGTTGGGTCTCTCACCGGTTCGTATCACTAATTTCTTCCATTTGCTCAGTTTCATTAAAGCTTAA

Coding sequence (CDS)

ATGCCCTCAGCCCAATTAGCCGAAGGCTGGAAAGGGGAATCCCCAATAATGTTCCTCCCATCTGACCCATCAAACAAACATATCCACATTTCTTCAGACCTTAACATAATATTCAACAACACCACATCTACCGTCTGCTCTCAAGGGGCATGGCAGCTCACCCCCGATGCTAACTCCGGTAAATTATACTTATCTACCGGAGGAAGTATCGGAAAACCGAGTTCGCAAACGACAGCTGCTAATTGGTTAAAGATAGAGAGAAGTAGTGTTGATTCGAGCTTTTATCAACTCGAGTATTGTCCTAGTAGTACTTCAACTAATATAGTGTGCGGCGTTATCGACGCTCTTGATTATACAGATGATTTCCGCTTGGTGTGGTTGGGTCTCTCACCGGTTCGTATCACTAATTTCTTCCATTTGCTCAGTTTCATTAAAGCTTAA

Protein sequence

MPSAQLAEGWKGESPIMFLPSDPSNKHIHISSDLNIIFNNTTSTVCSQGAWQLTPDANSGKLYLSTGGSIGKPSSQTTAANWLKIERSSVDSSFYQLEYCPSSTSTNIVCGVIDALDYTDDFRLVWLGLSPVRITNFFHLLSFIKA

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spo28443.1	Spo28443.1	mRNA

Homology

[Close]

BLAST of Spo28443.1 vs. NCBI nr
Match: gi|731344035|ref|XP_010683195.1| (PREDICTED: miraculin-like [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 184.1 bits (466), Expect = 1.700e-43
Identity = 97/148 (65.54%), Postives = 112/148 (75.68%), Query Frame = 1


		  
Query: 4   AQLAEGWKGESPIMFLPSDPSNKHIHISSDLNIIFNNTTSTVCSQGAWQLTPDANSGKLY 63
           AQLA G++GE P+ F PS  S K IHISSD+NIIF NTTS VCSQGAWQLTPDA++G LY
Sbjct: 77  AQLAYGFEGEIPVTFYPSSSSQKFIHISSDINIIF-NTTSNVCSQGAWQLTPDASNGNLY 136

Query: 64  LSTGGSIGKPSSQTTAANWLKIERSSVDSSFYQLEYCP-----SSTSTNIVCGVIDALDY 123
           LSTGG IG P S TT ANW KI +S  D +FYQLEYCP     +S + +IVCG IDALD 
Sbjct: 137 LSTGGVIGNPGSLTT-ANWFKIAKSPYDPNFYQLEYCPDTKTVASPTGDIVCGAIDALDS 196

Query: 124 TDDFRLVWLGLSPVRITNFFHLLSFIKA 147
           TDDF L+WLGL  +R  +FFH LSF+KA
Sbjct: 197 TDDFLLMWLGLKTIR-PDFFHWLSFVKA 221

[Close]

BLAST of Spo28443.1 vs. NCBI nr
Match: gi|731344031|ref|XP_010683193.1| (PREDICTED: miraculin-like [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 79.0 bits (193), Expect = 7.900e-12
Identity = 43/121 (35.54%), Postives = 65/121 (53.72%), Query Frame = 1


		  
Query: 15  PIMFLPSDPSNKHIHISSDLNIIFNNTTSTVCSQGAWQLTPDANSGKLYLSTGGSIGKPS 74
           P+ F P +P +K I +SSDLN +F+  T+ V S G W LT +  +G+ Y+ TGG IG P 
Sbjct: 83  PLKFYPVNPKDKRISLSSDLNFVFDAATTCVQSTG-WSLTIEMETGRRYVGTGGEIGNPG 142

Query: 75  SQTTAANWLKIERSSVDSSFYQLEYCPSSTS-TNIVCGVIDALDYTDDFRLVWLGLSPVR 134
            + T  NW KIE+       Y++ YCP   +   ++CG +      D  RL+     P+ 
Sbjct: 143 VE-TVDNWFKIEKDGSGKYDYKIVYCPGVCNFCKVMCGDVGVFIEKDGRRLLGFSDQPLL 201

[Close]

BLAST of Spo28443.1 vs. NCBI nr
Match: gi|902232969|gb|KNA22880.1| (hypothetical protein SOVF_029880 [Spinacia oleracea])

HSP 1 Score: 76.6 bits (187), Expect = 3.900e-11
Identity = 43/128 (33.59%), Postives = 73/128 (57.03%), Query Frame = 1


		  
Query: 15  PIMFLPSDPSNKHIHISSDLNIIFNNTTSTVCSQGAWQLTPDANSGKLYLSTGGSIGKPS 74
           P+ FLP++P  + + +SSDLNI+FN  ++ + S G WQLT D  +G+ Y++TGG+IG P 
Sbjct: 83  PVWFLPNNPRQRLVTLSSDLNILFNAISNCLQSVG-WQLTVDHTTGRKYVATGGAIGNPG 142

Query: 75  SQTTAANWLKIE-------RSSVDSSFYQLEYCPSSTS-TNIVCGVIDALDYTDDFRLVW 134
            + T ++W  IE       R +   + Y++ +CP+      ++CG +      D  RL+ 
Sbjct: 143 EE-TVSSWFNIEKVRTIGRRGNESGNHYKIVFCPNVCEFCMVMCGDVGVFVQEDGTRLLA 202

[Close]

BLAST of Spo28443.1 vs. NCBI nr
Match: gi|902194163|gb|KNA12470.1| (hypothetical protein SOVF_125760 [Spinacia oleracea])

HSP 1 Score: 74.7 bits (182), Expect = 1.500e-10
Identity = 43/122 (35.25%), Postives = 65/122 (53.28%), Query Frame = 1


		  
Query: 15  PIMFLPSDPSNKHIHISSDLNIIFNNTTSTVCSQG-AWQLTPDANSGKLYLSTGGSIGKP 74
           P+ F   +P +  I +S+DLN +F+   ST+C Q   W+LT D  +G+ Y+  GG IG P
Sbjct: 83  PLKFYSVNPKDTKIFVSTDLNFVFD--ASTICVQSNTWELTIDEETGRRYIGVGGEIGNP 142

Query: 75  SSQTTAANWLKIERSSVDSSFYQLEYCPSSTS-TNIVCGVIDALDYTDDFRLVWLGLSPV 134
             Q T +NW KIE++      Y++ YC    +   I+CG I A    D  RL+     P+
Sbjct: 143 GVQ-TVSNWFKIEKAGSGKYDYKIVYCAGVCNFCKIMCGDIGAFVEEDGRRLLGFSGQPL 201

[Close]

BLAST of Spo28443.1 vs. NCBI nr
Match: gi|731344033|ref|XP_010683194.1| (PREDICTED: miraculin-like [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 73.6 bits (179), Expect = 3.300e-10
Identity = 40/122 (32.79%), Postives = 65/122 (53.28%), Query Frame = 1


		  
Query: 15  PIMFLPSDPSNKHIHISSDLNIIFNNTTSTVCSQGA-WQLTPDANSGKLYLSTGGSIGKP 74
           P+ F P +P +K I +S+DLN +F+   +T+C Q   W+L  D  +G+ Y+  GG IG P
Sbjct: 85  PLKFYPVNPKDKTISLSTDLNFVFD--AATICVQSTQWRLAFDEVTGRRYVGFGGEIGNP 144

Query: 75  SSQTTAANWLKIERSSVDSSFYQLEYCPSSTS-TNIVCGVIDALDYTDDFRLVWLGLSPV 134
               T +NW KIE++      Y++ +CP   +   + CG I      D  RL+ +   P+
Sbjct: 145 GG-NTVSNWFKIEKAETGKYDYKIVFCPGVCNFCKVACGEIGVFVEKDGRRLLGINNQPL 203

[Close]

BLAST of Spo28443.1 vs. UniProtKB/TrEMBL
Match: A0A0J8C4E4_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_6g154010 PE=4 SV=1)

HSP 1 Score: 184.1 bits (466), Expect = 1.200e-43
Identity = 97/148 (65.54%), Postives = 112/148 (75.68%), Query Frame = 1


		  
Query: 4   AQLAEGWKGESPIMFLPSDPSNKHIHISSDLNIIFNNTTSTVCSQGAWQLTPDANSGKLY 63
           AQLA G++GE P+ F PS  S K IHISSD+NIIF NTTS VCSQGAWQLTPDA++G LY
Sbjct: 77  AQLAYGFEGEIPVTFYPSSSSQKFIHISSDINIIF-NTTSNVCSQGAWQLTPDASNGNLY 136

Query: 64  LSTGGSIGKPSSQTTAANWLKIERSSVDSSFYQLEYCP-----SSTSTNIVCGVIDALDY 123
           LSTGG IG P S TT ANW KI +S  D +FYQLEYCP     +S + +IVCG IDALD 
Sbjct: 137 LSTGGVIGNPGSLTT-ANWFKIAKSPYDPNFYQLEYCPDTKTVASPTGDIVCGAIDALDS 196

Query: 124 TDDFRLVWLGLSPVRITNFFHLLSFIKA 147
           TDDF L+WLGL  +R  +FFH LSF+KA
Sbjct: 197 TDDFLLMWLGLKTIR-PDFFHWLSFVKA 221

[Close]

BLAST of Spo28443.1 vs. UniProtKB/TrEMBL
Match: A0A0J8C0T9_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_6g153990 PE=4 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 5.500e-12
Identity = 43/121 (35.54%), Postives = 65/121 (53.72%), Query Frame = 1


		  
Query: 15  PIMFLPSDPSNKHIHISSDLNIIFNNTTSTVCSQGAWQLTPDANSGKLYLSTGGSIGKPS 74
           P+ F P +P +K I +SSDLN +F+  T+ V S G W LT +  +G+ Y+ TGG IG P 
Sbjct: 83  PLKFYPVNPKDKRISLSSDLNFVFDAATTCVQSTG-WSLTIEMETGRRYVGTGGEIGNPG 142

Query: 75  SQTTAANWLKIERSSVDSSFYQLEYCPSSTS-TNIVCGVIDALDYTDDFRLVWLGLSPVR 134
            + T  NW KIE+       Y++ YCP   +   ++CG +      D  RL+     P+ 
Sbjct: 143 VE-TVDNWFKIEKDGSGKYDYKIVYCPGVCNFCKVMCGDVGVFIEKDGRRLLGFSDQPLL 201

[Close]

BLAST of Spo28443.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RTK6_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_029880 PE=4 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 2.700e-11
Identity = 43/128 (33.59%), Postives = 73/128 (57.03%), Query Frame = 1


		  
Query: 15  PIMFLPSDPSNKHIHISSDLNIIFNNTTSTVCSQGAWQLTPDANSGKLYLSTGGSIGKPS 74
           P+ FLP++P  + + +SSDLNI+FN  ++ + S G WQLT D  +G+ Y++TGG+IG P 
Sbjct: 83  PVWFLPNNPRQRLVTLSSDLNILFNAISNCLQSVG-WQLTVDHTTGRKYVATGGAIGNPG 142

Query: 75  SQTTAANWLKIE-------RSSVDSSFYQLEYCPSSTS-TNIVCGVIDALDYTDDFRLVW 134
            + T ++W  IE       R +   + Y++ +CP+      ++CG +      D  RL+ 
Sbjct: 143 EE-TVSSWFNIEKVRTIGRRGNESGNHYKIVFCPNVCEFCMVMCGDVGVFVQEDGTRLLA 202

[Close]

BLAST of Spo28443.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QYY3_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_125760 PE=4 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 1.000e-10
Identity = 43/122 (35.25%), Postives = 65/122 (53.28%), Query Frame = 1


		  
Query: 15  PIMFLPSDPSNKHIHISSDLNIIFNNTTSTVCSQG-AWQLTPDANSGKLYLSTGGSIGKP 74
           P+ F   +P +  I +S+DLN +F+   ST+C Q   W+LT D  +G+ Y+  GG IG P
Sbjct: 83  PLKFYSVNPKDTKIFVSTDLNFVFD--ASTICVQSNTWELTIDEETGRRYIGVGGEIGNP 142

Query: 75  SSQTTAANWLKIERSSVDSSFYQLEYCPSSTS-TNIVCGVIDALDYTDDFRLVWLGLSPV 134
             Q T +NW KIE++      Y++ YC    +   I+CG I A    D  RL+     P+
Sbjct: 143 GVQ-TVSNWFKIEKAGSGKYDYKIVYCAGVCNFCKIMCGDIGAFVEEDGRRLLGFSGQPL 201

[Close]

BLAST of Spo28443.1 vs. UniProtKB/TrEMBL
Match: A0A0J8C4X4_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_6g154000 PE=4 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 2.300e-10
Identity = 40/122 (32.79%), Postives = 65/122 (53.28%), Query Frame = 1


		  
Query: 15  PIMFLPSDPSNKHIHISSDLNIIFNNTTSTVCSQGA-WQLTPDANSGKLYLSTGGSIGKP 74
           P+ F P +P +K I +S+DLN +F+   +T+C Q   W+L  D  +G+ Y+  GG IG P
Sbjct: 85  PLKFYPVNPKDKTISLSTDLNFVFD--AATICVQSTQWRLAFDEVTGRRYVGFGGEIGNP 144

Query: 75  SSQTTAANWLKIERSSVDSSFYQLEYCPSSTS-TNIVCGVIDALDYTDDFRLVWLGLSPV 134
               T +NW KIE++      Y++ +CP   +   + CG I      D  RL+ +   P+
Sbjct: 145 GG-NTVSNWFKIEKAETGKYDYKIVFCPGVCNFCKVACGEIGVFVEKDGRRLLGINNQPL 203

[Close]

BLAST of Spo28443.1 vs. ExPASy Swiss-Prot
Match: MIRA_SYNDU (Miraculin OS=Synsepalum dulcificum PE=1 SV=3)

HSP 1 Score: 52.8 bits (125), Expect = 3.800e-6
Identity = 36/107 (33.64%), Postives = 58/107 (54.21%), Query Frame = 1


		  
Query: 13  ESPIMFLPSDPSNKHIHISSDLNIIFNN------TTSTVCSQGAWQLTP-DANSGKLYLS 72
           + P+ F P +P    + +S+DLNI F+       T+STV     W+L   D ++G+ +++
Sbjct: 90  DRPLAFFPENPKEDVVRVSTDLNINFSAFMPCRWTSSTV-----WRLDKYDESTGQYFVT 149

Query: 73  TGGSIGKPSSQTTAANWLKIERSSVDSSFYQLEYCPS-STSTNIVCG 112
            GG  G P  + T ++W KIE     S FY+L +CP+   S  + CG
Sbjct: 150 IGGVKGNPGPE-TISSWFKIE-EFCGSGFYKLVFCPTVCGSCKVKCG 189

[Close]

BLAST of Spo28443.1 vs. TAIR (Arabidopsis)
Match: AT1G73325.1 (Kunitz family trypsin and protease inhibitor protein)

HSP 1 Score: 47.8 bits (112), Expect = 6.800e-6
Identity = 27/90 (30.00%), Postives = 44/90 (48.89%), Query Frame = 1


		  
Query: 15  PIMFLPSDPSNKHIHISSDLNIIFNNTTSTVCSQGAWQLTPDANSGKLYLSTGGSIGKPS 74
           P+ F P +   KH+ +S+ LN+ F++T         W++       K ++S GG  GK +
Sbjct: 89  PVTFSPLNTKVKHVQLSASLNLEFDSTVWLCPDSKVWRIDHSVQLRKSFVSIGGQKGKGN 148

Query: 75  SQTTAANWLKIERSSVDSSFYQLEYCPSST 105
           S      W +I+    D   Y+L YCP S+
Sbjct: 149 S------WFQIQE---DGDAYKLMYCPISS 169

The following BLAST results are available for this feature:

BLAST of Spo28443.1 vs. NCBI nr
BLAST of Spo28443.1 vs. UniProtKB/TrEMBL
BLAST of Spo28443.1 vs. ExPASy Swiss-Prot
BLAST of Spo28443.1 vs. TAIR (Arabidopsis)

BLAST of Spo28443.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|731344035\|ref\|XP_010683195.1\|	1.7e-43	65.5	PREDICTED: miraculin-like [Bet...	[more]
gi\|731344031\|ref\|XP_010683193.1\|	7.9e-12	35.5	PREDICTED: miraculin-like [Bet...	[more]
gi\|902232969\|gb\|KNA22880.1\|	3.9e-11	33.5	hypothetical protein SOVF_0298...	[more]
gi\|902194163\|gb\|KNA12470.1\|	1.5e-10	35.2	hypothetical protein SOVF_1257...	[more]
gi\|731344033\|ref\|XP_010683194.1\|	3.3e-10	32.7	PREDICTED: miraculin-like [Bet...	[more]

BLAST of Spo28443.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0J8C4E4_BETVU	1.2e-43	65.5	Uncharacterized protein OS=Bet...	[more]
A0A0J8C0T9_BETVU	5.5e-12	35.5	Uncharacterized protein OS=Bet...	[more]
A0A0K9RTK6_SPIOL	2.7e-11	33.5	Uncharacterized protein OS=Spi...	[more]
A0A0K9QYY3_SPIOL	1.0e-10	35.2	Uncharacterized protein OS=Spi...	[more]
A0A0J8C4X4_BETVU	2.3e-10	32.7	Uncharacterized protein OS=Bet...	[more]

BLAST of Spo28443.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 1


Match Name	E-value	Identity	Description
MIRA_SYNDU	3.8e-6	33.6	Miraculin OS=Synsepalum dulcif...	[more]

BLAST of Spo28443.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 1


Match Name	E-value	Identity	Description
AT1G73325.1	6.8e-6	30.0	Kunitz family trypsin and prot...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002160	Proteinase inhibitor I3, Kunitz legume	PFAM	PF00197	Kunitz_legume	coord: 12..124 score: 1.2
IPR011065	Kunitz inhibitor ST1-like	unknown	SSF50386	STI-like	coord: 14..126 score: 2.06
None	No IPR available	GENE3D	2.80.10.50		coord: 14..124 score: 2.9
None	No IPR available	PANTHER	PTHR33107	FAMILY NOT NAMED	coord: 15..142 score: 3.3

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0044249	cellular biosynthetic process
biological_process	GO:0006631	fatty acid metabolic process
biological_process	GO:1901576	organic substance biosynthetic process
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0004866	endopeptidase inhibitor activity
molecular_function	GO:0005488	binding
molecular_function	GO:0016747	transferase activity, transferring acyl groups other than amino-acyl groups

RNA-Seq Expression

Co-expression


Gene	r value	Expression
Spo10768	0.93	Barchart \| Table
Spo21150	0.65	Barchart \| Table