Spo28474 (gene) | SpinachBase

Overview


Name	Spo28474
Type	gene
Organism	Spinacia oleracea (Spinach)
Description	Trypsin inhibitor
Location	SpoScf_45977 : 1 .. 717 (+)

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GCAATCACTCTTCTTGTGTTTCTTTCTCCTCTTCCTACACTCAACGCCGTTGTTCTCGACACCGACGGTGAGGATGTACGTAACAGCGGCGGATCCTATTACATCATCCCAAAAGGAAGTAGTAACGGCCTCACTCTTACCCAGAGACCCGGGAGCCCATCATGCCCACTCTATATAACCCAGATAACCACCTCTCCGGGTACATCTGTAACCATCTCTGCTCTTGTGAGAACACTTTTCATTCCAGTTTCCGCTAGAGTTAACATCGTGTTCAACACTGACGTTGCCACGTGCGCGGACCCGCTTGAGTGGCACGTGACCCCTGATTTGGCTACCGGGAAGTCTTATGTGACTGCTGGTGGTGGTTCGACGGTTTCCAGTTATAGATTCAGCATTGAGCAGGCTGGCAATGGTGAAAAGGACAATATTTATAAGATAAGGTACTGTCCGTCTGCTGAGGAAGATGAAACAAATTGTGGTGACGTGGGTTTTCTCGGGGAGAATGGATTATTGGGTATCAATAATGAGAATCCACTTTTCGTTCAGTTTAAGAAAGTTAATACTGGAATGTCTGAGATAGTGTAATAATCTTCTCATTTACTGCTGCTTTTTCTTGGAATTATGTCGTCAATAATAATGGATTAATGGGTATAAACTATGAATGTATAATGCCTGTAAGTTTATTACAATAAGGAGAACTCTCTGTCTCGTGTTAAA

mRNA sequence

GCAATCACTCTTCTTGTGTTTCTTTCTCCTCTTCCTACACTCAACGCCGTTGTTCTCGACACCGACGGTGAGGATGTACGTAACAGCGGCGGATCCTATTACATCATCCCAAAAGGAAGTAGTAACGGCCTCACTCTTACCCAGAGACCCGGGAGCCCATCATGCCCACTCTATATAACCCAGATAACCACCTCTCCGGGTACATCTGTAACCATCTCTGCTCTTGTGAGAACACTTTTCATTCCAGTTTCCGCTAGAGTTAACATCGTGTTCAACACTGACGTTGCCACGTGCGCGGACCCGCTTGAGTGGCACGTGACCCCTGATTTGGCTACCGGGAAGTCTTATGTGACTGCTGGTGGTGGTTCGACGGTTTCCAGTTATAGATTCAGCATTGAGCAGGCTGGCAATGGTGAAAAGGACAATATTTATAAGATAAGGTACTGTCCGTCTGCTGAGGAAGATGAAACAAATTGTGGTGACGTGGGTTTTCTCGGGGAGAATGGATTATTGGGTATCAATAATGAGAATCCACTTTTCGTTCAGTTTAAGAAAGTTAATACTGGAATGTCTGAGATAGTGTAATAATCTTCTCATTTACTGCTGCTTTTTCTTGGAATTATGTCGTCAATAATAATGGATTAATGGGTATAAACTATGAATGTATAATGCCTGTAAGTTTATTACAATAAGGAGAACTCTCTGTCTCGTGTTAAA

Coding sequence (CDS)

GCAATCACTCTTCTTGTGTTTCTTTCTCCTCTTCCTACACTCAACGCCGTTGTTCTCGACACCGACGGTGAGGATGTACGTAACAGCGGCGGATCCTATTACATCATCCCAAAAGGAAGTAGTAACGGCCTCACTCTTACCCAGAGACCCGGGAGCCCATCATGCCCACTCTATATAACCCAGATAACCACCTCTCCGGGTACATCTGTAACCATCTCTGCTCTTGTGAGAACACTTTTCATTCCAGTTTCCGCTAGAGTTAACATCGTGTTCAACACTGACGTTGCCACGTGCGCGGACCCGCTTGAGTGGCACGTGACCCCTGATTTGGCTACCGGGAAGTCTTATGTGACTGCTGGTGGTGGTTCGACGGTTTCCAGTTATAGATTCAGCATTGAGCAGGCTGGCAATGGTGAAAAGGACAATATTTATAAGATAAGGTACTGTCCGTCTGCTGAGGAAGATGAAACAAATTGTGGTGACGTGGGTTTTCTCGGGGAGAATGGATTATTGGGTATCAATAATGAGAATCCACTTTTCGTTCAGTTTAAGAAAGTTAATACTGGAATGTCTGAGATAGTGTAA

Protein sequence

AITLLVFLSPLPTLNAVVLDTDGEDVRNSGGSYYIIPKGSSNGLTLTQRPGSPSCPLYITQITTSPGTSVTISALVRTLFIPVSARVNIVFNTDVATCADPLEWHVTPDLATGKSYVTAGGGSTVSSYRFSIEQAGNGEKDNIYKIRYCPSAEEDETNCGDVGFLGENGLLGINNENPLFVQFKKVNTGMSEIV

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Spo28474.1	Spo28474.1	mRNA

Homology

[Close]

BLAST of Spo28474.1 vs. NCBI nr
Match: gi|902204418|gb|KNA14861.1| (hypothetical protein SOVF_103530 [Spinacia oleracea])

HSP 1 Score: 387.1 bits (993), Expect = 1.800e-104
Identity = 194/194 (100.00%), Postives = 194/194 (100.00%), Query Frame = 1


		  
Query: 1   AITLLVFLSPLPTLNAVVLDTDGEDVRNSGGSYYIIPKGSSNGLTLTQRPGSPSCPLYIT 60
           AITLLVFLSPLPTLNAVVLDTDGEDVRNSGGSYYIIPKGSSNGLTLTQRPGSPSCPLYIT
Sbjct: 10  AITLLVFLSPLPTLNAVVLDTDGEDVRNSGGSYYIIPKGSSNGLTLTQRPGSPSCPLYIT 69

Query: 61  QITTSPGTSVTISALVRTLFIPVSARVNIVFNTDVATCADPLEWHVTPDLATGKSYVTAG 120
           QITTSPGTSVTISALVRTLFIPVSARVNIVFNTDVATCADPLEWHVTPDLATGKSYVTAG
Sbjct: 70  QITTSPGTSVTISALVRTLFIPVSARVNIVFNTDVATCADPLEWHVTPDLATGKSYVTAG 129

Query: 121 GGSTVSSYRFSIEQAGNGEKDNIYKIRYCPSAEEDETNCGDVGFLGENGLLGINNENPLF 180
           GGSTVSSYRFSIEQAGNGEKDNIYKIRYCPSAEEDETNCGDVGFLGENGLLGINNENPLF
Sbjct: 130 GGSTVSSYRFSIEQAGNGEKDNIYKIRYCPSAEEDETNCGDVGFLGENGLLGINNENPLF 189

Query: 181 VQFKKVNTGMSEIV 195
           VQFKKVNTGMSEIV
Sbjct: 190 VQFKKVNTGMSEIV 203

[Close]

BLAST of Spo28474.1 vs. NCBI nr
Match: gi|902232970|gb|KNA22881.1| (hypothetical protein SOVF_029890 [Spinacia oleracea])

HSP 1 Score: 271.9 bits (694), Expect = 8.400e-70
Identity = 138/194 (71.13%), Postives = 156/194 (80.41%), Query Frame = 1


		  
Query: 1   AITLLVFLSPLPTLNAVVLDTDGEDVRNSGGSYYIIPKGSSNGLTLTQRPGSPSCPLYIT 60
           AIT LVFLS LP+LNA VLD+DGE VRNSGGSYYIIPKGSSNGL+L QR GSPSCPLYIT
Sbjct: 8   AITFLVFLSALPSLNAAVLDSDGEIVRNSGGSYYIIPKGSSNGLSLKQRSGSPSCPLYIT 67

Query: 61  QITTSPGTSVTISALVRTLFIPVSARVNIVFNTDVATCADPLEWHVTPDLATGKSYVTAG 120
           + + SPGT VTI +  R+LFIPVSA+V I+F  +V +C D LEW VT DL+TGK YV AG
Sbjct: 68  RKSNSPGTPVTIFSPFRSLFIPVSAKVKIIFQANVTSCTDSLEWRVTSDLSTGKPYVVAG 127

Query: 121 GGS-TVSSYRFSIEQAGNGEKDNIYKIRYCPSAEEDETNCGDVGFLGENGLLGINNENPL 180
           GG  +V SY FSIEQAGNGE DN+YKI YCP   ED+ NCGDVGFL  +GLLGINNE PL
Sbjct: 128 GGGPSVFSYFFSIEQAGNGEMDNVYKINYCPLNGEDDKNCGDVGFLRRSGLLGINNEKPL 187

Query: 181 FVQFKKVNTGMSEI 194
            +QFK  NTGMSE+
Sbjct: 188 LLQFKNANTGMSEM 201

[Close]

BLAST of Spo28474.1 vs. NCBI nr
Match: gi|731344392|ref|XP_010683391.1| (PREDICTED: trypsin inhibitor 2-like [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 189.9 bits (481), Expect = 4.200e-45
Identity = 107/194 (55.15%), Postives = 132/194 (68.04%), Query Frame = 1


		  
Query: 2   ITLLVFLSPLPTLNAVVLDTDGEDVRNSGGSYYIIPKGSSNGLTLTQRPGSPS-CPLYIT 61
           ITLL+F  PLPT++  VLD DGE +RN GGSY ++PKG   GLTLTQ+P + S CP YIT
Sbjct: 10  ITLLLFFHPLPTISTFVLDIDGEPLRN-GGSYNVVPKGFHGGLTLTQKPDNNSPCPFYIT 69

Query: 62  QITTSPGTSVTISALVRTLFIPVSARVNIVF-NTDVATCADPLEWHVTPDLATGKSYVTA 121
           + T  PG  V IS+ ++ L+IP+S  VNIVF N +  TC +PL W VTPD+ TG+ YV  
Sbjct: 70  RKTACPGIPVKISSPLKVLYIPLSVDVNIVFTNNNATTCMEPLGWRVTPDVTTGQFYVAT 129

Query: 122 GGGSTVSSYRFSIEQAG-NGEKDN-IYKIRYCPSAE-EDETNCGDVGFLGENGLLGINNE 181
           GG STV +  F+IE+A  NG  +   YK+RYCPS E  DE  CGDV F  E G +GI NE
Sbjct: 130 GGTSTVVTDFFTIEEADENGRSEKYAYKLRYCPSFEGGDEKKCGDVNFFRE-GFVGITNE 189

Query: 182 NPLFVQFKKVNTGM 191
            PLFVQFKK N  +
Sbjct: 190 EPLFVQFKKANANL 201

[Close]

BLAST of Spo28474.1 vs. NCBI nr
Match: gi|731364459|ref|XP_010693983.1| (PREDICTED: factor Xa inhibitor BuXI-like [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 127.1 bits (318), Expect = 3.300e-26
Identity = 85/189 (44.97%), Postives = 108/189 (57.14%), Query Frame = 1


		  
Query: 3   TLLVFLSPLPTLNAVVLDTDGEDVRNSGGSYYIIPK--GSSNGLTLTQRPGSPSCPLYIT 62
           TL++  S L    AV+LDTDG+ +RN GG YYIIP+  G   GLTL  + G+  CPLYIT
Sbjct: 11  TLILIFSALCIATAVILDTDGDALRN-GGQYYIIPRSLGFGGGLTLNSKAGNTPCPLYIT 70

Query: 63  QIT--TSPGTSVTISALVRTLFIPVSARVNIVFNTDVATCADPLEWHVTPDLATGKSYVT 122
           +    TSPG +VTI++  R   I  S  ++IVFN     C  PL W V PD  TG+SYV 
Sbjct: 71  RENDETSPGIAVTIASPARIGIITSSLPISIVFNDIPNICMQPLWWQVIPDGTTGQSYVA 130

Query: 123 AGGGSTV--SSYRFSIEQAGNGEKDNIYKIRYCPSAEEDETNCGDVGFLGENGLLGINNE 182
            GG       +  FSIE      + NIYKIR    +++      DVGF   +GLLGI N+
Sbjct: 131 TGGSGIPFNPTETFSIEPI----EGNIYKIRNGVGSDQ----ARDVGFFENDGLLGITND 190

Query: 183 NPLFVQFKK 186
            PL V F+K
Sbjct: 191 IPLPVVFRK 190

[Close]

BLAST of Spo28474.1 vs. NCBI nr
Match: gi|731364403|ref|XP_010693954.1| (PREDICTED: trypsin inhibitor 1B-like [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 126.7 bits (317), Expect = 4.400e-26
Identity = 80/194 (41.24%), Postives = 108/194 (55.67%), Query Frame = 1


		  
Query: 3   TLLVFLSPLPTLNAVVLDTDGEDVRNSGGSYYIIPK--GSSNGLTLTQRPGSPSCPLYIT 62
           TLL+  S L    A +LDTDGE + NS   YYI+P+  G   GLT T +     CP YIT
Sbjct: 11  TLLLIFSTLSIATAFILDTDGEPLLNSR-KYYILPQSLGFGGGLTRTTKNTDLPCPYYIT 70

Query: 63  QIT--TSPGTSVTISALVRTLFIPVSARVNIVFNTDVATCADPLEWHVTPDLATGKSYVT 122
           +    TS G  + IS+  R L+ P+S+ V I F   +  C   + W + PD +TG+SYV 
Sbjct: 71  RDNDETSNGMPLIISSPFRILYTPLSSPVYIAFEEMITVCIQSMGWRLIPDDSTGRSYVG 130

Query: 123 AGGGSTVSSYRFSIEQAGNGEKDNIYKIRYCPSAEEDETNCGDVGFLGENGLLGINNENP 182
            G      + RF+IE AG+  ++N+YKIRY   + E      DVGF  E+GLLGI ++ P
Sbjct: 131 TGASGFDLTQRFTIEHAGSEFENNVYKIRYIGESGEGR----DVGFFKEDGLLGITDDIP 190

Query: 183 LFVQFKKVNTGMSE 193
           L V FKK    + E
Sbjct: 191 LTVMFKKAFNVLEE 199

[Close]

BLAST of Spo28474.1 vs. UniProtKB/TrEMBL
Match: A0A0K9R5S6_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_103530 PE=4 SV=1)

HSP 1 Score: 387.1 bits (993), Expect = 1.300e-104
Identity = 194/194 (100.00%), Postives = 194/194 (100.00%), Query Frame = 1


		  
Query: 1   AITLLVFLSPLPTLNAVVLDTDGEDVRNSGGSYYIIPKGSSNGLTLTQRPGSPSCPLYIT 60
           AITLLVFLSPLPTLNAVVLDTDGEDVRNSGGSYYIIPKGSSNGLTLTQRPGSPSCPLYIT
Sbjct: 10  AITLLVFLSPLPTLNAVVLDTDGEDVRNSGGSYYIIPKGSSNGLTLTQRPGSPSCPLYIT 69

Query: 61  QITTSPGTSVTISALVRTLFIPVSARVNIVFNTDVATCADPLEWHVTPDLATGKSYVTAG 120
           QITTSPGTSVTISALVRTLFIPVSARVNIVFNTDVATCADPLEWHVTPDLATGKSYVTAG
Sbjct: 70  QITTSPGTSVTISALVRTLFIPVSARVNIVFNTDVATCADPLEWHVTPDLATGKSYVTAG 129

Query: 121 GGSTVSSYRFSIEQAGNGEKDNIYKIRYCPSAEEDETNCGDVGFLGENGLLGINNENPLF 180
           GGSTVSSYRFSIEQAGNGEKDNIYKIRYCPSAEEDETNCGDVGFLGENGLLGINNENPLF
Sbjct: 130 GGSTVSSYRFSIEQAGNGEKDNIYKIRYCPSAEEDETNCGDVGFLGENGLLGINNENPLF 189

Query: 181 VQFKKVNTGMSEIV 195
           VQFKKVNTGMSEIV
Sbjct: 190 VQFKKVNTGMSEIV 203

[Close]

BLAST of Spo28474.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RTK4_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_029890 PE=4 SV=1)

HSP 1 Score: 271.9 bits (694), Expect = 5.900e-70
Identity = 138/194 (71.13%), Postives = 156/194 (80.41%), Query Frame = 1


		  
Query: 1   AITLLVFLSPLPTLNAVVLDTDGEDVRNSGGSYYIIPKGSSNGLTLTQRPGSPSCPLYIT 60
           AIT LVFLS LP+LNA VLD+DGE VRNSGGSYYIIPKGSSNGL+L QR GSPSCPLYIT
Sbjct: 8   AITFLVFLSALPSLNAAVLDSDGEIVRNSGGSYYIIPKGSSNGLSLKQRSGSPSCPLYIT 67

Query: 61  QITTSPGTSVTISALVRTLFIPVSARVNIVFNTDVATCADPLEWHVTPDLATGKSYVTAG 120
           + + SPGT VTI +  R+LFIPVSA+V I+F  +V +C D LEW VT DL+TGK YV AG
Sbjct: 68  RKSNSPGTPVTIFSPFRSLFIPVSAKVKIIFQANVTSCTDSLEWRVTSDLSTGKPYVVAG 127

Query: 121 GGS-TVSSYRFSIEQAGNGEKDNIYKIRYCPSAEEDETNCGDVGFLGENGLLGINNENPL 180
           GG  +V SY FSIEQAGNGE DN+YKI YCP   ED+ NCGDVGFL  +GLLGINNE PL
Sbjct: 128 GGGPSVFSYFFSIEQAGNGEMDNVYKINYCPLNGEDDKNCGDVGFLRRSGLLGINNEKPL 187

Query: 181 FVQFKKVNTGMSEI 194
            +QFK  NTGMSE+
Sbjct: 188 LLQFKNANTGMSEM 201

[Close]

BLAST of Spo28474.1 vs. UniProtKB/TrEMBL
Match: A0A0J8BFA5_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_3g069460 PE=4 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 2.300e-26
Identity = 85/189 (44.97%), Postives = 108/189 (57.14%), Query Frame = 1


		  
Query: 3   TLLVFLSPLPTLNAVVLDTDGEDVRNSGGSYYIIPK--GSSNGLTLTQRPGSPSCPLYIT 62
           TL++  S L    AV+LDTDG+ +RN GG YYIIP+  G   GLTL  + G+  CPLYIT
Sbjct: 11  TLILIFSALCIATAVILDTDGDALRN-GGQYYIIPRSLGFGGGLTLNSKAGNTPCPLYIT 70

Query: 63  QIT--TSPGTSVTISALVRTLFIPVSARVNIVFNTDVATCADPLEWHVTPDLATGKSYVT 122
           +    TSPG +VTI++  R   I  S  ++IVFN     C  PL W V PD  TG+SYV 
Sbjct: 71  RENDETSPGIAVTIASPARIGIITSSLPISIVFNDIPNICMQPLWWQVIPDGTTGQSYVA 130

Query: 123 AGGGSTV--SSYRFSIEQAGNGEKDNIYKIRYCPSAEEDETNCGDVGFLGENGLLGINNE 182
            GG       +  FSIE      + NIYKIR    +++      DVGF   +GLLGI N+
Sbjct: 131 TGGSGIPFNPTETFSIEPI----EGNIYKIRNGVGSDQ----ARDVGFFENDGLLGITND 190

Query: 183 NPLFVQFKK 186
            PL V F+K
Sbjct: 191 IPLPVVFRK 190

[Close]

BLAST of Spo28474.1 vs. UniProtKB/TrEMBL
Match: A0A0J8E666_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_3g069440 PE=4 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 3.000e-26
Identity = 80/194 (41.24%), Postives = 108/194 (55.67%), Query Frame = 1


		  
Query: 3   TLLVFLSPLPTLNAVVLDTDGEDVRNSGGSYYIIPK--GSSNGLTLTQRPGSPSCPLYIT 62
           TLL+  S L    A +LDTDGE + NS   YYI+P+  G   GLT T +     CP YIT
Sbjct: 11  TLLLIFSTLSIATAFILDTDGEPLLNSR-KYYILPQSLGFGGGLTRTTKNTDLPCPYYIT 70

Query: 63  QIT--TSPGTSVTISALVRTLFIPVSARVNIVFNTDVATCADPLEWHVTPDLATGKSYVT 122
           +    TS G  + IS+  R L+ P+S+ V I F   +  C   + W + PD +TG+SYV 
Sbjct: 71  RDNDETSNGMPLIISSPFRILYTPLSSPVYIAFEEMITVCIQSMGWRLIPDDSTGRSYVG 130

Query: 123 AGGGSTVSSYRFSIEQAGNGEKDNIYKIRYCPSAEEDETNCGDVGFLGENGLLGINNENP 182
            G      + RF+IE AG+  ++N+YKIRY   + E      DVGF  E+GLLGI ++ P
Sbjct: 131 TGASGFDLTQRFTIEHAGSEFENNVYKIRYIGESGEGR----DVGFFKEDGLLGITDDIP 190

Query: 183 LFVQFKKVNTGMSE 193
           L V FKK    + E
Sbjct: 191 LTVMFKKAFNVLEE 199

[Close]

BLAST of Spo28474.1 vs. UniProtKB/TrEMBL
Match: A0A0J8E675_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_3g069530 PE=4 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 7.500e-25
Identity = 83/191 (43.46%), Postives = 110/191 (57.59%), Query Frame = 1


		  
Query: 2   ITLLVFLSPLPTLNAVVLDTDGEDVRN--SGGSYYIIPK--GSSNGLTLTQRPGSPSCPL 61
           ITLL+  S L    AVVLDTDGE + N  +   YYI+P+  G   GLT T +     CP 
Sbjct: 10  ITLLLIFSTLSVAIAVVLDTDGEPLFNQVNNSRYYILPQTIGIGGGLTRTTKNPELPCPY 69

Query: 62  YITQIT--TSPGTSVTISALVRTLFIPVSARVNIVFNTDVATCA-DPLEWHVTPDLATGK 121
           YIT+    TS G  + IS+ ++ LFIP+S+ V I F      C  + + W V  D +TG+
Sbjct: 70  YITRDNDETSSGMPLYISSPLKILFIPLSSPVRISFEEMPTICIQESMGWRVISDDSTGR 129

Query: 122 SYVTAGGGSTVSSYRFSIEQAGNGEKDNIYKIRYCPSAEEDETNCGDVGFLGENGLLGIN 181
            YV+ G G      RF+IEQA +   +N+YKIRY   A + E   GD+GF  E+GLLGI 
Sbjct: 130 LYVSTGAGRGPG--RFTIEQAESESSNNVYKIRYI-GALDSEAGAGDLGFFKEDGLLGIT 189

Query: 182 NENPLFVQFKK 186
           ++ PL V FKK
Sbjct: 190 DDIPLTVVFKK 197

[Close]

BLAST of Spo28474.1 vs. ExPASy Swiss-Prot
Match: ITRY_PHAVA (Trypsin inhibitor BvTI OS=Phanera variegata PE=1 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 8.000e-20
Identity = 75/178 (42.13%), Postives = 98/178 (55.06%), Query Frame = 1


		  
Query: 18  VLDTDGEDVRNSGGSYYIIP--KGSSNGLTLTQRPGSPSCPLYITQITT--SPGTSVTIS 77
           +LDTDGE VRN+GG YYIIP  +G+  GLTLT R GS +CP  + Q ++  S G  V IS
Sbjct: 3   LLDTDGEVVRNNGGPYYIIPAFRGNGGGLTLT-RVGSETCPRTVVQASSEHSDGLPVVIS 62

Query: 78  ALVRTLFIPVSARVNIVFNTDVATCAD-PLEWHVTPDLATGKSYVTAGGGSTVSSYRFSI 137
           AL R+LFI  S RV I F    ATC   P  WH+  D    +  V  G         F I
Sbjct: 63  ALPRSLFISTSWRVTIQFVE--ATCIPKPSFWHIPQDSEL-EGAVKVGASDERFPLEFRI 122

Query: 138 EQAGNGEKDNIYKIRYCPSAEEDETNCGDVGF-LGENG--LLGINNENPLFVQFKKVN 188
           E+      ++ YK+ +C S  +   +C D+G  + E G   L + +ENPL V+FKK N
Sbjct: 123 ERVS----EDTYKLMHCSSTSD---SCRDLGISIDEEGNRRLVVRDENPLLVRFKKAN 169

[Close]

BLAST of Spo28474.1 vs. ExPASy Swiss-Prot
Match: IT1B_PSOTE (Trypsin inhibitor 1B OS=Psophocarpus tetragonolobus PE=1 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 5.700e-10
Identity = 57/177 (32.20%), Postives = 87/177 (49.15%), Query Frame = 1


		  
Query: 18  VLDTDGEDVRNSGGSYYIIP-KGSSNGLTLTQRPGSPSCPLYITQITT--SPGTSVTISA 77
           +LD++GE VRN GG+YY++P + +  G       G+ +CPL + +     S G  + IS+
Sbjct: 3   LLDSEGELVRN-GGTYYLLPDRWALGGGIEAAATGTETCPLTVVRSPNEVSVGEPLRISS 62

Query: 78  LVRTLFIPVSARVNIVFNTDVATCADPLEWHVTPDLATGKSYVTAGGGSTVSSYRFSIEQ 137
            +R+ FIP  + V I F  +   CA    W V  D     S   +   ST   Y F  E+
Sbjct: 63  QLRSGFIPDYSLVRIGF-ANPPKCAPSPWWTVVEDQPQQPSVKLSELKSTKFDYLFKFEK 122

Query: 138 AGNGEKDNIYKIRYCPSAEEDETNCGDVGFLGEN---GLLGINNENPLFVQFKKVNT 189
             +  K + YK++YC   +     C D+G   +      L + +ENPL V FKKV +
Sbjct: 123 VTS--KFSSYKLKYCAKRD----TCKDIGIYRDQKGYARLVVTDENPLVVIFKKVES 171

[Close]

BLAST of Spo28474.1 vs. ExPASy Swiss-Prot
Match: IT1A_PSOTE (Trypsin inhibitor 1A OS=Psophocarpus tetragonolobus PE=1 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 9.800e-10
Identity = 58/177 (32.77%), Postives = 89/177 (50.28%), Query Frame = 1


		  
Query: 18  VLDTDGEDVRNSGGSYYIIP-KGSSNGLTLTQRPGSPSCPLYITQITT--SPGTSVTISA 77
           +LD++GE VRN GG+YY++P + +  G       G+ +CPL + +     S G  + IS+
Sbjct: 3   LLDSEGELVRN-GGTYYLLPDRWALGGGIEAAATGTETCPLTVVRSPNEVSVGEPLRISS 62

Query: 78  LVRTLFIPVSARVNIVFNTDVATCADPLEWHVTPDLATGKSYVTAGGGSTVSSYRFSIEQ 137
            +R+ FIP  + V I F  +   CA    W V  D     S   +   ST   Y F  E+
Sbjct: 63  QLRSGFIPDYSVVRIGF-ANPPKCAPSPWWTVVEDQPQQPSVKLSELKSTKFDYLFKFEK 122

Query: 138 AGNGEKDNIYKIRYCPSAEEDETNCGDVG-FLGENGL--LGINNENPLFVQFKKVNT 189
             +  K + YK++YC   +     C D+G +  + G   L + +ENPL V FKKV +
Sbjct: 123 VTS--KFSSYKLKYCAKRD----TCKDIGIYRDQKGYERLVVTDENPLVVIFKKVES 171

[Close]

BLAST of Spo28474.1 vs. ExPASy Swiss-Prot
Match: ITRY_LEULE (Trypsin inhibitor OS=Leucaena leucocephala PE=1 SV=2)

HSP 1 Score: 52.0 bits (123), Expect = 8.600e-6
Identity = 59/190 (31.05%), Postives = 84/190 (44.21%), Query Frame = 1


		  
Query: 17  VVLDTDGEDVRNSGGSYYIIP--KGSSNGLTLTQRPGSPSCPLYITQIT--TSPGTSVTI 76
           V++D DG+ + N G SYYI+P  +G   GL L  R GS SCP  + Q    TS G    +
Sbjct: 2   VLVDLDGDPLYN-GMSYYILPVARGKGGGLELA-RTGSESCPRTVVQTRSETSRGLPARL 61

Query: 77  SALVRTLFIPVSARVNIVFNTDVA-TC----ADPLEWHVTPD----LATGKSYVTAGGGS 136
           ++  R L I  +  + I F      +C    +  L+W V       +A+        G  
Sbjct: 62  ASPYRIL-IGSNIPLTIEFQPQKPYSCHGHSSRSLQWKVEKTQMVKIASSDEEQRLFGPF 121

Query: 137 TVSSYRFSIEQAGNGEKDNIYKIRYCPSAEEDE-TNCGDVGFL---GENGLLGINNENPL 190
            +  YR            N YK+ YC S   +   +C D+G      +N LL + N +PL
Sbjct: 122 QIQPYR------------NHYKLVYCESESRNHHDDCRDLGISIDDQQNRLLVVKNGDPL 176

The following BLAST results are available for this feature:

BLAST of Spo28474.1 vs. NCBI nr
BLAST of Spo28474.1 vs. UniProtKB/TrEMBL
BLAST of Spo28474.1 vs. ExPASy Swiss-Prot
BLAST of Spo28474.1 vs. TAIR (Arabidopsis)

BLAST of Spo28474.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902204418\|gb\|KNA14861.1\|	1.8e-104	100.	hypothetical protein SOVF_1035...	[more]
gi\|902232970\|gb\|KNA22881.1\|	8.4e-70	71.1	hypothetical protein SOVF_0298...	[more]
gi\|731344392\|ref\|XP_010683391.1\|	4.2e-45	55.1	PREDICTED: trypsin inhibitor 2...	[more]
gi\|731364459\|ref\|XP_010693983.1\|	3.3e-26	44.9	PREDICTED: factor Xa inhibitor...	[more]
gi\|731364403\|ref\|XP_010693954.1\|	4.4e-26	41.2	PREDICTED: trypsin inhibitor 1...	[more]

BLAST of Spo28474.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9R5S6_SPIOL	1.3e-104	100.	Uncharacterized protein OS=Spi...	[more]
A0A0K9RTK4_SPIOL	5.9e-70	71.1	Uncharacterized protein OS=Spi...	[more]
A0A0J8BFA5_BETVU	2.3e-26	44.9	Uncharacterized protein OS=Bet...	[more]
A0A0J8E666_BETVU	3.0e-26	41.2	Uncharacterized protein OS=Bet...	[more]
A0A0J8E675_BETVU	7.5e-25	43.4	Uncharacterized protein OS=Bet...	[more]

BLAST of Spo28474.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 4


Match Name	E-value	Identity	Description
ITRY_PHAVA	8.0e-20	42.1	Trypsin inhibitor BvTI OS=Phan...	[more]
IT1B_PSOTE	5.7e-10	32.2	Trypsin inhibitor 1B OS=Psopho...	[more]
IT1A_PSOTE	9.8e-10	32.7	Trypsin inhibitor 1A OS=Psopho...	[more]
ITRY_LEULE	8.6e-6	31.0	Trypsin inhibitor OS=Leucaena ...	[more]

BLAST of Spo28474.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 0


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002160	Proteinase inhibitor I3, Kunitz legume	PFAM	PF00197	Kunitz_legume	coord: 18..185 score: 8.1
IPR002160	Proteinase inhibitor I3, Kunitz legume	SMART	SM00452	kul_2	coord: 17..187 score: 5.6
IPR011065	Kunitz inhibitor ST1-like	unknown	SSF50386	STI-like	coord: 17..186 score: 3.92
None	No IPR available	GENE3D	2.80.10.50		coord: 17..187 score: 7.8
None	No IPR available	PANTHER	PTHR33107	FAMILY NOT NAMED	coord: 4..191 score: 4.3

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0050830	defense response to Gram-positive bacterium
biological_process	GO:0050832	defense response to fungus
molecular_function	GO:0004866	endopeptidase inhibitor activity
molecular_function	GO:0008061	chitin binding

RNA-Seq Expression

Co-expression


Gene	r value	Expression
Spo28224	0.66	Barchart \| Table
Spo28225	0.66	Barchart \| Table