Spo00553.1 (mRNA) | SpinachBase

Overview


Name	Spo00553.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	(Coiled-coil-helix-coiled-coil-helix domain containing protein) (Intermembrane space import and assembly protein)
Location	chr1 : 31353886 .. 31357910 (-)
Sequence length	978

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GGACTATGTGACTCACTGTAGTCACTGTAGTCGTGTTCCCTTGCTCGCTGGAACGAGGTTACTTCTCTGGATCAATTGTCAACTTCTTTTGTTACTTTCTGTGCAGCAACCAGCAACGAAGCTAGAAAATGGGTCAATCTCAAAGTGATTCGTCCGCGTCCACCATTGATGAGACCCCAAAGGAGAACTCAAATGCCCCACCTTCTTCCTTGGAATCTCTAATTGCAGGTCTGTTTCCTCCAAATTTTTTTGGGTTTCGTTTAATTTTCCATCTGCTTCTTTATTAGAAATTGAATTGCACTTCTCTGCAAAATTATCCTCCTTACTTTATTGATTTTCTGTTAAAGGTAACACTTAACTTGAGAAGGGGACAGTAAATTCATTCTGGGGATTTTAGTTGATAATAATAATTTGGAATCGGTTTTGGCTGATAAATTTTAATCCCTTTTCCGCAGTTAATAATGAGGTTGAAGATTGATCAGAGTTCAGATTATTTTTTGAGAAAAAATGTGACATGGTTTTTGCCGGAAATTATGAGCTAGAAGAACAATTTCTTTAATTGGGTATCATCAAATTCACAAATTTCACTTAAATTCCAAATATCTGAAATTTAGTGTTTCAAATTTTACAGTAAATTTTATGCAATTTAGAGACGAGTACTTCACAGAAAATACATGTGGATTATGAACACTTTCTAATGAGTACTGTATAAGTATAACATAACATTTCAACAACTTTTAGTTTTATTACTTTGTACTCCAGTTCTTATTTTAAACTGAAGTCTCCCTAAGTTAGCACCAAGTTTAGGATGTCAATTTAAGCTTCCATAGGATGCTTTCTTGAAGGTTGTAAGTTTGGGTCATGGAATGAAGCGAAAGTATGGGGTATGGATGTATTGTAGATGAGAATAGGCTTTGGTTTTACTTTTTGTGTTATTATTTTTGTCAAATGATTGAAACTGTCTGCAATGTTCTTGGAATCTTAAAGAGTTAAAGAAATGGTTTCTGGTTGGTCACTTGGTAGAAAGTCTAAAAACCTTGTAGGCTTTGAACCTCTTGAAATGTTGACTGTCGTTTTTCCTTAAATAGAAATATGACACACGACTGTCTTTGATTTCTGCTATCATTGACTATAGGCTTCATTCTATGCTGATCTAGACGAGGGCTGGCTTTTTAGTAAGCTTGTTTCCGCAGAACTTGGTCATGGGTCGTGGAGGGATGAATCATATTCTTTCTCGAGGTTATCTTGTAAATTTTTCCTCCATTCAGTCGTGTATGTTGGTTGCCTCTGCACTGACCGAGAATGCAAAGCACTTTGATTCAAAATATAGTGTCGCTGACTGATTTAGGGTTTCAAAAGGGGAGGCCCAAGTTATAGATGTTGCTTTAAGAACTCCAATTCTCCCCCTTATTAGCAGCCAAAGATGGCCTCTCTAGGCCCTGTTCCCACACTTCATCAAAGTGTTTTCGCCCTTTCCACACTTGTCAATTTAGCAGGCTTGCTATGATCTGGTTATATATACTATACCATCATTATCATTACCCCATTGCCTCAAAGAGGCTCCCGCAAGAAGCGGGGTAAGGAGGGGTCGGGTGTACGCAACCTTACCCCTGCAATCGCAGAAAGGTTTCCAATTGACCCAAAATAGATGACGGGACGACAACGGGAAGACCATCTTCTACTTCATGGAAAGGAAGTGAAGAGGTTTTAAGAGCCATTTTGATTTAGTCCATTACATCCCACAGCCAAAAGAACAAATGAATCTTTGGCTCCGTCGGAAATGGACAAATTTGGTTACTATGATAAAATAAAATAAAAACCTGTTTTAAATACTAGTATACATGTAAAAAGAAGAATTGGACCAGAAAATATTGGCATAGGGAAGGTTGTGTAGATAGCTATCCTTTGTAAATGGTTTCTAGATTGAAAGATTACTTTGGCACTTAGGCTACAAACCCATTGGAAGGCATGTGCTTGACTTGCTTGGGTGTATGGTTGCATAGGGAAGCCTGGTTGTACTGAATGCGTAACCGAATAATGACAGGAGTTGGGAATTACAACAATTTCTCGATTAGAAAAATAAAGACAGCGACAATGATTTTCACTTGTTATATGAAATTGATTATTCTAGGATTTCTATCTATGATTTTTGCATGTTGTTTTCAGTGAGAATATGATTCCTATTTGAACCTTATACAATTTCCTTTTTGGCATTGCAGAGGCAGCAGCATACGGTGATAGCGAGAATGAGGTCATTAAGCTCTACTGTTGAACAGTTGAAGTATCTTCTTTGTTATATATTCAAGAAAGTTTTTCTCCTTGTATTATTTTGTTCTTTCCTAAATGAGTTGTGTGTTCTTTTCAGTCTATTGATGCAAAAGCTAGAAAAGCGTTGGAATGCCCATGTATAGCTGACTTGCGAGAAGGCTCATGTGGGAGTCAATTCTCAGAGGCCTTCCTCTGTTTTCTTAAGAGCACTGCTGAAGAAAAGGTGAGTGCACATAACAGACAACAATTATAGTTTCTCCATAAACAGCTTTTAACATATACTTCCTCCGTTTGAAAATAGATTCTACATAGAGAAGTGGTACATTATCTAAGAAAGTTAGAAAGATTGTAACGAGCCACATTTTGGCATATCAATTCCACCTTTAACCCCCCAAATTTGGTCCCCACCTTTTAGTATGAGGGCATGTTTTAGTTTAAGGTACCTTTCCAAAACTAATACTTCGTATGTGGGAATCATTGTGACAGCTCTGTATATGGGAAGTGTAGCAATTATTATGAAACAAAGGAAGTTTGCACCCAAAAAAATTATGAAAAAGAGGAAGTAATACGTTTGAATAGCATATGCAGTGATGCATGTATTGGTTTTATTTGTGAAATCTAATTTAGTTCAAGTGATAACTTTCAACTCTTAAATGGAAACGTTTGATTAATTTTTTCGAGAATAATGGTATGATAATTTATTTGGATATAATAGACTTAATCAAAATGGCTCTCAAAACCTCTTCGCTTCTTTTCCATGAAGTAGAAGATGGTCGTCCCGTTATTGTTTATGGGTAAATTAGAAACAACTTCTTTGCTGCAATACAGGGTAAGGTTGCGTACGTCCGACCACCCCTTACCACGCTTGTAGCGGGAGCCTCTTTGAAGGCACTGTGGTAATGATAATGAATGGTATGATCATTTATTTTTATGGTACGTTGGAGGTGAGGCAAGGGTGGAGATGGTGTGAGGACGGGGAAGCAAATCATGTGTGATAAAACTCGAGAAGTTTTCATCCTTTGATATGTTTGTTCGCAAATTTGGTTTGGTTATGGATCTTTGGTTGAAATGGAATTCTCGATTTCACTTCATTTTGTTGAGTAACAGAGGTTATGTCTTTCGTTTATTCATGATATAATATTGGCTTTTGTTTGTCTGTTTTCAGGGCTCCGACTGTGTGCATCCGTTTGTGGCATTGCAAAGTTGTATAAAGGCCAATCCAAATGCATTTTCAAAGGATATTGTGGGAGATGATAAAGTCAAGGAAGAAGTGTCTTCTGCAAATTACAGAATACATCCACCAAAATGGTCTTTAGAATCCCAAAGTCCTAAACCTAAACTGTAAACCGTAGAGAAAATTATTCACCCCAAAATTTGCGCAAAACTTCCTTTGTCTATTGGCATGCATGTTTTATGAGTAGTAAAGAACAATCTAGCACCACGGCTTGTTGTATGCAGGTTGATTGCTCGAGGATAGAAATGAAAAGATAGCTGAAATGCTCTTTTGAGAACTGTGTAACAACGAATAATCTCATACAAAGCGTTGTGACACTTAATTTGCTTCGACTAGTTACGCTTTCTTGTACACAAAGTGAACTTACTTCACAGTAGTAAAAAAATAAGCATTTATAATATAATCTCTCTAATCGACCATGAGGAAGATTAATGTTCCAACTCACAAAGATGAACTGAAATGAAGAGAAGATAAAGCTGCTTCAATAAGCAGAAAATGTGTTGAAAATTGAAGTACCCCTCTG

mRNA sequence

GGACTATGTGACTCACTGTAGTCACTGTAGTCGTGTTCCCTTGCTCGCTGGAACGAGGTTACTTCTCTGGATCAATTGTCAACTTCTTTTGTTACTTTCTGTGCAGCAACCAGCAACGAAGCTAGAAAATGGGTCAATCTCAAAGTGATTCGTCCGCGTCCACCATTGATGAGACCCCAAAGGAGAACTCAAATGCCCCACCTTCTTCCTTGGAATCTCTAATTGCAGAGGCAGCAGCATACGGTGATAGCGAGAATGAGTCTATTGATGCAAAAGCTAGAAAAGCGTTGGAATGCCCATGTATAGCTGACTTGCGAGAAGGCTCATGTGGGAGTCAATTCTCAGAGGCCTTCCTCTGTTTTCTTAAGAGCACTGCTGAAGAAAAGGGCTCCGACTGTGTGCATCCGTTTGTGGCATTGCAAAGTTGTATAAAGGCCAATCCAAATGCATTTTCAAAGGATATTGTGGGAGATGATAAAGTCAAGGAAGAAGTGTCTTCTGCAAATTACAGAATACATCCACCAAAATGGTCTTTAGAATCCCAAAGTCCTAAACCTAAACTGTAAACCGTAGAGAAAATTATTCACCCCAAAATTTGCGCAAAACTTCCTTTGTCTATTGGCATGCATGTTTTATGAGTAGTAAAGAACAATCTAGCACCACGGCTTGTTGTATGCAGGTTGATTGCTCGAGGATAGAAATGAAAAGATAGCTGAAATGCTCTTTTGAGAACTGTGTAACAACGAATAATCTCATACAAAGCGTTGTGACACTTAATTTGCTTCGACTAGTTACGCTTTCTTGTACACAAAGTGAACTTACTTCACAGTAGTAAAAAAATAAGCATTTATAATATAATCTCTCTAATCGACCATGAGGAAGATTAATGTTCCAACTCACAAAGATGAACTGAAATGAAGAGAAGATAAAGCTGCTTCAATAAGCAGAAAATGTGTTGAAAATTGAAGTACCCCTCTG

Coding sequence (CDS)

ATGGGTCAATCTCAAAGTGATTCGTCCGCGTCCACCATTGATGAGACCCCAAAGGAGAACTCAAATGCCCCACCTTCTTCCTTGGAATCTCTAATTGCAGAGGCAGCAGCATACGGTGATAGCGAGAATGAGTCTATTGATGCAAAAGCTAGAAAAGCGTTGGAATGCCCATGTATAGCTGACTTGCGAGAAGGCTCATGTGGGAGTCAATTCTCAGAGGCCTTCCTCTGTTTTCTTAAGAGCACTGCTGAAGAAAAGGGCTCCGACTGTGTGCATCCGTTTGTGGCATTGCAAAGTTGTATAAAGGCCAATCCAAATGCATTTTCAAAGGATATTGTGGGAGATGATAAAGTCAAGGAAGAAGTGTCTTCTGCAAATTACAGAATACATCCACCAAAATGGTCTTTAGAATCCCAAAGTCCTAAACCTAAACTGTAA

Protein sequence

MGQSQSDSSASTIDETPKENSNAPPSSLESLIAEAAAYGDSENESIDAKARKALECPCIADLREGSCGSQFSEAFLCFLKSTAEEKGSDCVHPFVALQSCIKANPNAFSKDIVGDDKVKEEVSSANYRIHPPKWSLESQSPKPKL

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo00553	Spo00553	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo00553.1	Spo00553.1-protein	polypeptide

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo00553.1.utr5p.1	Spo00553.1.utr5p.1	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo00553.1.CDS.4	Spo00553.1.CDS.4	CDS
Spo00553.1.CDS.3	Spo00553.1.CDS.3	CDS
Spo00553.1.CDS.2	Spo00553.1.CDS.2	CDS
Spo00553.1.CDS.1	Spo00553.1.CDS.1	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo00553.1.utr3p.1	Spo00553.1.utr3p.1	three_prime_UTR

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo00553.1.exon.4	Spo00553.1.exon.4	exon
Spo00553.1.exon.3	Spo00553.1.exon.3	exon
Spo00553.1.exon.2	Spo00553.1.exon.2	exon
Spo00553.1.exon.1	Spo00553.1.exon.1	exon

Homology

[Close]

BLAST of Spo00553.1 vs. NCBI nr
Match: gi|902208675|gb|KNA16007.1| (hypothetical protein SOVF_093080 [Spinacia oleracea])

HSP 1 Score: 285.8 bits (730), Expect = 4.200e-74
Identity = 145/145 (100.00%), Postives = 145/145 (100.00%), Query Frame = 1


		  
Query: 1   MGQSQSDSSASTIDETPKENSNAPPSSLESLIAEAAAYGDSENESIDAKARKALECPCIA 60
           MGQSQSDSSASTIDETPKENSNAPPSSLESLIAEAAAYGDSENESIDAKARKALECPCIA
Sbjct: 1   MGQSQSDSSASTIDETPKENSNAPPSSLESLIAEAAAYGDSENESIDAKARKALECPCIA 60

Query: 61  DLREGSCGSQFSEAFLCFLKSTAEEKGSDCVHPFVALQSCIKANPNAFSKDIVGDDKVKE 120
           DLREGSCGSQFSEAFLCFLKSTAEEKGSDCVHPFVALQSCIKANPNAFSKDIVGDDKVKE
Sbjct: 61  DLREGSCGSQFSEAFLCFLKSTAEEKGSDCVHPFVALQSCIKANPNAFSKDIVGDDKVKE 120

Query: 121 EVSSANYRIHPPKWSLESQSPKPKL 146
           EVSSANYRIHPPKWSLESQSPKPKL
Sbjct: 121 EVSSANYRIHPPKWSLESQSPKPKL 145

[Close]

BLAST of Spo00553.1 vs. NCBI nr
Match: gi|731315988|ref|XP_010693769.1| (PREDICTED: mitochondrial intermembrane space import and assembly protein 40 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 244.2 bits (622), Expect = 1.400e-61
Identity = 124/145 (85.52%), Postives = 136/145 (93.79%), Query Frame = 1


		  
Query: 1   MGQSQSDSSASTIDETPKENSNAPPSSLESLIAEAAAYGDSENESIDAKARKALECPCIA 60
           MGQSQSD+SA  IDET KEN+NAPPSS+ESLIAEAAAYGD ENESIDAKA+KALECPCIA
Sbjct: 1   MGQSQSDTSA--IDETSKENTNAPPSSMESLIAEAAAYGDDENESIDAKAQKALECPCIA 60

Query: 61  DLREGSCGSQFSEAFLCFLKSTAEEKGSDCVHPFVALQSCIKANPNAFSKDIVGDDKVKE 120
           DLR+GSCGSQFSEAFLCFLKSTAEEKGSDCV+PF+ALQSCIK+NP+AF KDI GDD+VKE
Sbjct: 61  DLRKGSCGSQFSEAFLCFLKSTAEEKGSDCVNPFLALQSCIKSNPDAFPKDITGDDQVKE 120

Query: 121 EVSSANYRIHPPKWSLESQSPKPKL 146
           E SSANY I PPKW+LESQSPKP+L
Sbjct: 121 EESSANYSILPPKWALESQSPKPRL 143

[Close]

BLAST of Spo00553.1 vs. NCBI nr
Match: gi|1012339261|gb|KYP50497.1| (hypothetical protein KK1_027749 [Cajanus cajan])

HSP 1 Score: 196.4 bits (498), Expect = 3.400e-47
Identity = 101/145 (69.66%), Postives = 117/145 (80.69%), Query Frame = 1


		  
Query: 1   MGQSQSDSSASTIDETPKENSNAPPSSLESLIAEAAAYGDSENESIDAKARKALECPCIA 60
           MGQ++S  + +    T  ++SN  PSSLES IAEAAAYG  + ES++A A+KALECPCIA
Sbjct: 1   MGQTESAEAVAETQSTTNDSSN--PSSLESAIAEAAAYGSQDTESVEAMAQKALECPCIA 60

Query: 61  DLREGSCGSQFSEAFLCFLKSTAEEKGSDCVHPFVALQSCIKANPNAFSKDIVGDDKVKE 120
           DLR GSCGSQFSEAFLCFLKST EEKGSDCVHPFVALQSCIKANP+AFSKDI+G+D+ KE
Sbjct: 61  DLRTGSCGSQFSEAFLCFLKSTTEEKGSDCVHPFVALQSCIKANPDAFSKDILGEDESKE 120

Query: 121 EVSSANYRIHPPKWSLESQSPKPKL 146
                 Y+I PPKWS ESQSPK +L
Sbjct: 121 LEPIQEYKILPPKWSKESQSPKSRL 143

[Close]

BLAST of Spo00553.1 vs. NCBI nr
Match: gi|568828719|ref|XP_006468687.1| (PREDICTED: mitochondrial intermembrane space import and assembly protein 40 [Citrus sinensis])

HSP 1 Score: 186.8 bits (473), Expect = 2.700e-44
Identity = 103/153 (67.32%), Postives = 121/153 (79.08%), Query Frame = 1


		  
Query: 1   MGQSQSDSSASTIDETPKENSN--APPSS-----LESLIAEAAAYGDSENESIDAKARKA 60
           MGQ QS++    ID+T  E S+  +P SS     +ESLIAEAAAYGD ENES+D KA+KA
Sbjct: 1   MGQVQSEA----IDQTEGEQSSPSSPDSSQRAASIESLIAEAAAYGDDENESLDEKAQKA 60

Query: 61  LECPCIADLREGSCGSQFSEAFLCFLKSTAEEKGSDCVHPFVALQSCIKANPNAFSKDIV 120
           LECPCIADLR G CG+QFSEAFLCFLKST+EEKGSDCVHPFVALQ+CIKANP+AFSKDI+
Sbjct: 61  LECPCIADLRSGPCGAQFSEAFLCFLKSTSEEKGSDCVHPFVALQNCIKANPSAFSKDIL 120

Query: 121 GDDKVK-EEVSSANYRIHPPKWSLESQSPKPKL 146
            +D+VK EE     Y+I PP WS E ++PK KL
Sbjct: 121 EEDEVKNEEEPPPEYKIIPPVWSKEPRTPKSKL 149

[Close]

BLAST of Spo00553.1 vs. NCBI nr
Match: gi|703126391|ref|XP_010103569.1| (hypothetical protein L484_023064 [Morus notabilis])

HSP 1 Score: 186.8 bits (473), Expect = 2.700e-44
Identity = 101/160 (63.12%), Postives = 121/160 (75.62%), Query Frame = 1


		  
Query: 1   MGQSQSDSSASTIDE--------------TPKENSNAPPSSLESLIAEAAAYGDSENESI 60
           MGQ QSD ++   +E               P  +S++  SS+ESLIAEAAAYG+   ES+
Sbjct: 1   MGQVQSDVASEASNEHVPGGDRILSSSSAKPTSSSSSSSSSMESLIAEAAAYGNDGEESL 60

Query: 61  DAKARKALECPCIADLREGSCGSQFSEAFLCFLKSTAEEKGSDCVHPFVALQSCIKANPN 120
           +AKA+KALECPCIA LR GSCG QFSEAFLCFLKSTAEEKGSDCVHPFVALQ+CIKANP+
Sbjct: 61  EAKAQKALECPCIAHLRSGSCGVQFSEAFLCFLKSTAEEKGSDCVHPFVALQNCIKANPD 120

Query: 121 AFSKDIVGDDKV-KEEVSSANYRIHPPKWSLESQSPKPKL 146
           AFSKDI+ DD+V KEE  +  Y+I+PP+WS E +SPK KL
Sbjct: 121 AFSKDILEDDEVKKEEQPTQEYKIYPPEWSKEPRSPKSKL 160

[Close]

BLAST of Spo00553.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RB46_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_093080 PE=4 SV=1)

HSP 1 Score: 285.8 bits (730), Expect = 2.900e-74
Identity = 145/145 (100.00%), Postives = 145/145 (100.00%), Query Frame = 1


		  
Query: 1   MGQSQSDSSASTIDETPKENSNAPPSSLESLIAEAAAYGDSENESIDAKARKALECPCIA 60
           MGQSQSDSSASTIDETPKENSNAPPSSLESLIAEAAAYGDSENESIDAKARKALECPCIA
Sbjct: 1   MGQSQSDSSASTIDETPKENSNAPPSSLESLIAEAAAYGDSENESIDAKARKALECPCIA 60

Query: 61  DLREGSCGSQFSEAFLCFLKSTAEEKGSDCVHPFVALQSCIKANPNAFSKDIVGDDKVKE 120
           DLREGSCGSQFSEAFLCFLKSTAEEKGSDCVHPFVALQSCIKANPNAFSKDIVGDDKVKE
Sbjct: 61  DLREGSCGSQFSEAFLCFLKSTAEEKGSDCVHPFVALQSCIKANPNAFSKDIVGDDKVKE 120

Query: 121 EVSSANYRIHPPKWSLESQSPKPKL 146
           EVSSANYRIHPPKWSLESQSPKPKL
Sbjct: 121 EVSSANYRIHPPKWSLESQSPKPKL 145

[Close]

BLAST of Spo00553.1 vs. UniProtKB/TrEMBL
Match: A0A0J8D370_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_2g027620 PE=4 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 9.800e-62
Identity = 124/145 (85.52%), Postives = 136/145 (93.79%), Query Frame = 1


		  
Query: 1   MGQSQSDSSASTIDETPKENSNAPPSSLESLIAEAAAYGDSENESIDAKARKALECPCIA 60
           MGQSQSD+SA  IDET KEN+NAPPSS+ESLIAEAAAYGD ENESIDAKA+KALECPCIA
Sbjct: 1   MGQSQSDTSA--IDETSKENTNAPPSSMESLIAEAAAYGDDENESIDAKAQKALECPCIA 60

Query: 61  DLREGSCGSQFSEAFLCFLKSTAEEKGSDCVHPFVALQSCIKANPNAFSKDIVGDDKVKE 120
           DLR+GSCGSQFSEAFLCFLKSTAEEKGSDCV+PF+ALQSCIK+NP+AF KDI GDD+VKE
Sbjct: 61  DLRKGSCGSQFSEAFLCFLKSTAEEKGSDCVNPFLALQSCIKSNPDAFPKDITGDDQVKE 120

Query: 121 EVSSANYRIHPPKWSLESQSPKPKL 146
           E SSANY I PPKW+LESQSPKP+L
Sbjct: 121 EESSANYSILPPKWALESQSPKPRL 143

[Close]

BLAST of Spo00553.1 vs. UniProtKB/TrEMBL
Match: A0A151S6V8_CAJCA (Uncharacterized protein OS=Cajanus cajan GN=KK1_027749 PE=4 SV=1)

HSP 1 Score: 196.4 bits (498), Expect = 2.300e-47
Identity = 101/145 (69.66%), Postives = 117/145 (80.69%), Query Frame = 1


		  
Query: 1   MGQSQSDSSASTIDETPKENSNAPPSSLESLIAEAAAYGDSENESIDAKARKALECPCIA 60
           MGQ++S  + +    T  ++SN  PSSLES IAEAAAYG  + ES++A A+KALECPCIA
Sbjct: 1   MGQTESAEAVAETQSTTNDSSN--PSSLESAIAEAAAYGSQDTESVEAMAQKALECPCIA 60

Query: 61  DLREGSCGSQFSEAFLCFLKSTAEEKGSDCVHPFVALQSCIKANPNAFSKDIVGDDKVKE 120
           DLR GSCGSQFSEAFLCFLKST EEKGSDCVHPFVALQSCIKANP+AFSKDI+G+D+ KE
Sbjct: 61  DLRTGSCGSQFSEAFLCFLKSTTEEKGSDCVHPFVALQSCIKANPDAFSKDILGEDESKE 120

Query: 121 EVSSANYRIHPPKWSLESQSPKPKL 146
                 Y+I PPKWS ESQSPK +L
Sbjct: 121 LEPIQEYKILPPKWSKESQSPKSRL 143

[Close]

BLAST of Spo00553.1 vs. UniProtKB/TrEMBL
Match: W9RWY3_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_023064 PE=4 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 1.900e-44
Identity = 101/160 (63.12%), Postives = 121/160 (75.62%), Query Frame = 1


		  
Query: 1   MGQSQSDSSASTIDE--------------TPKENSNAPPSSLESLIAEAAAYGDSENESI 60
           MGQ QSD ++   +E               P  +S++  SS+ESLIAEAAAYG+   ES+
Sbjct: 1   MGQVQSDVASEASNEHVPGGDRILSSSSAKPTSSSSSSSSSMESLIAEAAAYGNDGEESL 60

Query: 61  DAKARKALECPCIADLREGSCGSQFSEAFLCFLKSTAEEKGSDCVHPFVALQSCIKANPN 120
           +AKA+KALECPCIA LR GSCG QFSEAFLCFLKSTAEEKGSDCVHPFVALQ+CIKANP+
Sbjct: 61  EAKAQKALECPCIAHLRSGSCGVQFSEAFLCFLKSTAEEKGSDCVHPFVALQNCIKANPD 120

Query: 121 AFSKDIVGDDKV-KEEVSSANYRIHPPKWSLESQSPKPKL 146
           AFSKDI+ DD+V KEE  +  Y+I+PP+WS E +SPK KL
Sbjct: 121 AFSKDILEDDEVKKEEQPTQEYKIYPPEWSKEPRSPKSKL 160

[Close]

BLAST of Spo00553.1 vs. UniProtKB/TrEMBL
Match: A0A067GNM5_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g031983mg PE=4 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 1.900e-44
Identity = 103/153 (67.32%), Postives = 121/153 (79.08%), Query Frame = 1


		  
Query: 1   MGQSQSDSSASTIDETPKENSN--APPSS-----LESLIAEAAAYGDSENESIDAKARKA 60
           MGQ QS++    ID+T  E S+  +P SS     +ESLIAEAAAYGD ENES+D KA+KA
Sbjct: 1   MGQVQSEA----IDQTEGEQSSPSSPDSSQRAASIESLIAEAAAYGDDENESLDEKAQKA 60

Query: 61  LECPCIADLREGSCGSQFSEAFLCFLKSTAEEKGSDCVHPFVALQSCIKANPNAFSKDIV 120
           LECPCIADLR G CG+QFSEAFLCFLKST+EEKGSDCVHPFVALQ+CIKANP+AFSKDI+
Sbjct: 61  LECPCIADLRSGPCGAQFSEAFLCFLKSTSEEKGSDCVHPFVALQNCIKANPSAFSKDIL 120

Query: 121 GDDKVK-EEVSSANYRIHPPKWSLESQSPKPKL 146
            +D+VK EE     Y+I PP WS E ++PK KL
Sbjct: 121 EEDEVKNEEEPPPEYKIIPPVWSKEPRTPKSKL 149

[Close]

BLAST of Spo00553.1 vs. ExPASy Swiss-Prot
Match: MIA40_USTMA (Mitochondrial intermembrane space import and assembly protein 40 OS=Ustilago maydis (strain 521 / FGSC 9021) GN=MIA40 PE=3 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 9.500e-10
Identity = 41/111 (36.94%), Postives = 57/111 (51.35%), Query Frame = 1


		  
Query: 7   DSSASTIDETPKENSNAPPSSLESLIAEAAAYGDSENESIDAKARKALECPCIADLREGS 66
           D     ++ETP     A   S E    + +AY D E   I+       +CPC+  +  G 
Sbjct: 108 DERVEPVEETPVAIEVAVEES-EEQTGQQSAY-DPETGEIN------WDCPCLGGMAHGP 167

Query: 67  CGSQFSEAFLCFLKSTAEEKGSDCVHPFVALQSCIKANPNAFSKDIVGDDK 118
           CG QF  AF CF+ S AE KG DCV  F A+Q C + +P+ + KD + DD+
Sbjct: 168 CGEQFKLAFSCFVYSEAEPKGIDCVDKFKAMQDCFREHPDVY-KDEIEDDE 209

[Close]

BLAST of Spo00553.1 vs. ExPASy Swiss-Prot
Match: MIA40_SCHPO (Mitochondrial intermembrane space import and assembly protein 40 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=mia40 PE=3 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 1.600e-9
Identity = 31/88 (35.23%), Postives = 52/88 (59.09%), Query Frame = 1


		  
Query: 55  ECPCIADLREGSCGSQFSEAFLCFLKSTAEEKGSDCVHPFVALQSCIKANPNAFSKDIVG 114
           +CPC+  +  G CG +F  AF CF+ S +E KG +C+  F A+Q+C + +P  + +D+VG
Sbjct: 198 DCPCLGGMAHGPCGEEFKAAFSCFVYSKSEPKGMECLDKFQAMQACFQKHPEIY-QDMVG 257

Query: 115 DDKVKEEVSSANYRIHPPKWSLESQSPK 143
           +   +EE +  N +  P   S E+  P+
Sbjct: 258 ES--EEEDAETNEK--PSTTSDENNQPQ 280

[Close]

BLAST of Spo00553.1 vs. ExPASy Swiss-Prot
Match: MIA40_ASHGO (Mitochondrial intermembrane space import and assembly protein 40 OS=Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) GN=MIA40 PE=3 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 2.800e-9
Identity = 32/125 (25.60%), Postives = 59/125 (47.20%), Query Frame = 1


		  
Query: 1   MGQSQSDSSASTIDETPKENSNAPPSSLESLIAEAAAYGDSENESIDAKARKALECPCIA 60
           +G++Q++ + +   E   E   A  ++ E   +   A    +            +CPC+ 
Sbjct: 132 VGETQAEQAPAVETEQAAEAEQAAEAAAEDKASAGEAAQGQQGAYNPDTGEINWDCPCLG 191

Query: 61  DLREGSCGSQFSEAFLCFLKSTAEEKGSDCVHPFVALQSCIKANPNAFSKDIVGDDKVKE 120
            +  G CG +F  AF CF+ S AE KG DCV  F  +Q C + +P  +++ +  +++   
Sbjct: 192 GMAHGPCGEEFKAAFACFVYSEAEPKGIDCVEKFQVMQDCFRQHPEHYAEQLESEEQAVR 251

Query: 121 EVSSA 126
           E  +A
Sbjct: 252 ETEAA 256

[Close]

BLAST of Spo00553.1 vs. ExPASy Swiss-Prot
Match: MIA40_YEAST (Mitochondrial intermembrane space import and assembly protein 40 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=MIA40 PE=1 SV=2)

HSP 1 Score: 62.0 bits (149), Expect = 6.200e-9
Identity = 40/148 (27.03%), Postives = 62/148 (41.89%), Query Frame = 1


		  
Query: 3   QSQSDSSASTIDETPKENSNAPPSSLESLIAEAAAYGDSENESIDAKARKAL-------- 62
           Q QSD   +T D   +E S+    S     A+ +   D E E +  +  K +        
Sbjct: 221 QGQSDDKTTTEDNNGEEESSKKTVSDSENSAKQSESSDEEKEELRKQEEKQMGPTEEEVQ 280

Query: 63  --------------ECPCIADLREGSCGSQFSEAFLCFLKSTAEEKGSDCVHPFVALQSC 122
                         +CPC+  +  G CG +F  AF CF+ S AE KG DCV  F  +Q C
Sbjct: 281 HEGAYNPDTGEINWDCPCLGGMAHGPCGEEFKSAFSCFVYSEAEPKGIDCVEKFQHMQDC 340

Query: 123 IKANPNAFSKDI--VGDDKVKEEVSSAN 127
            +  P  +++ +    DD+  ++    N
Sbjct: 341 FRKYPEHYAEQLKETSDDEEPQDKVKVN 368

[Close]

BLAST of Spo00553.1 vs. ExPASy Swiss-Prot
Match: MIA40_KLULA (Mitochondrial intermembrane space import and assembly protein 40 OS=Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37) GN=MIA40 PE=3 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 8.100e-9
Identity = 37/129 (28.68%), Postives = 62/129 (48.06%), Query Frame = 1


		  
Query: 5   QSDSSASTIDETPKE------NSNAPPSSLESLIAEAAAYGDSENESIDAKARKALE--- 64
           +S + A TI E  ++      +      S  +  A A   G+ +NE   A   +  E   
Sbjct: 221 KSPAEAETITEAEEQAEIRSISGGTAEQSATAAAAAAGVQGEKKNEQQTAYNPETGEINW 280

Query: 65  -CPCIADLREGSCGSQFSEAFLCFLKSTAEEKGSDCVHPFVALQSCIKANPNAFSKDIVG 124
            CPC+  +  G CG +F  AF CF+ S A+ KG +CV  F  +Q+C +  P+ +++ I  
Sbjct: 281 DCPCLGGMAYGPCGEEFKSAFSCFVYSEADPKGINCVEKFSTMQNCFRKYPDYYAEQIKD 340

[Close]

BLAST of Spo00553.1 vs. TAIR (Arabidopsis)
Match: AT5G23395.1 (Cox19-like CHCH family protein)

HSP 1 Score: 168.3 bits (425), Expect = 3.500e-42
Identity = 91/151 (60.26%), Postives = 116/151 (76.82%), Query Frame = 1


		  
Query: 1   MGQSQSDSSA---STIDETPKENSNAPPSS-------LESLIAEAAAYG--DSENESIDA 60
           MGQ+QSD ++   +T   TP  ++N+P  S       ++SL+AEAAAYG  D+ENES++A
Sbjct: 1   MGQAQSDENSIPTTTTTNTPPPSANSPRDSEDTSSPSMDSLLAEAAAYGEDDNENESLEA 60

Query: 61  KARKALECPCIADLREGSCGSQFSEAFLCFLKSTAEEKGSDCVHPFVALQSCIKANPNAF 120
           KA++AL+CPCIADLR GSCGSQFSEAFLCFLKSTAEEKGSDCV+PFVALQSCI ANP+AF
Sbjct: 61  KAQRALDCPCIADLRNGSCGSQFSEAFLCFLKSTAEEKGSDCVNPFVALQSCINANPDAF 120

Query: 121 SKDIVGD----DKVKEEVSSANYRIHPPKWS 136
           SK + GD    +K +E+    ++RI PP W+
Sbjct: 121 SKSVTGDEKETEKKEEQPPVQDHRIIPPLWA 151

The following BLAST results are available for this feature:

BLAST of Spo00553.1 vs. NCBI nr
BLAST of Spo00553.1 vs. UniProtKB/TrEMBL
BLAST of Spo00553.1 vs. ExPASy Swiss-Prot
BLAST of Spo00553.1 vs. TAIR (Arabidopsis)

BLAST of Spo00553.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902208675\|gb\|KNA16007.1\|	4.2e-74	100.	hypothetical protein SOVF_0930...	[more]
gi\|731315988\|ref\|XP_010693769.1\|	1.4e-61	85.5	PREDICTED: mitochondrial inter...	[more]
gi\|1012339261\|gb\|KYP50497.1\|	3.4e-47	69.6	hypothetical protein KK1_02774...	[more]
gi\|568828719\|ref\|XP_006468687.1\|	2.7e-44	67.3	PREDICTED: mitochondrial inter...	[more]
gi\|703126391\|ref\|XP_010103569.1\|	2.7e-44	63.1	hypothetical protein L484_0230...	[more]

BLAST of Spo00553.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9RB46_SPIOL	2.9e-74	100.	Uncharacterized protein OS=Spi...	[more]
A0A0J8D370_BETVU	9.8e-62	85.5	Uncharacterized protein OS=Bet...	[more]
A0A151S6V8_CAJCA	2.3e-47	69.6	Uncharacterized protein OS=Caj...	[more]
W9RWY3_9ROSA	1.9e-44	63.1	Uncharacterized protein OS=Mor...	[more]
A0A067GNM5_CITSI	1.9e-44	67.3	Uncharacterized protein OS=Cit...	[more]

BLAST of Spo00553.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
MIA40_USTMA	9.5e-10	36.9	Mitochondrial intermembrane sp...	[more]
MIA40_SCHPO	1.6e-9	35.2	Mitochondrial intermembrane sp...	[more]
MIA40_ASHGO	2.8e-9	25.6	Mitochondrial intermembrane sp...	[more]
MIA40_YEAST	6.2e-9	27.0	Mitochondrial intermembrane sp...	[more]
MIA40_KLULA	8.1e-9	28.6	Mitochondrial intermembrane sp...	[more]

BLAST of Spo00553.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 1


Match Name	E-value	Identity	Description
AT5G23395.1	3.5e-42	60.2	Cox19-like CHCH family protein	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR010625	CHCH	PFAM	PF06747	CHCH	coord: 67..102 score: 1.
None	No IPR available	PANTHER	PTHR21622	COILED-COIL-HELIX-COILED-COIL-HELIX DOMAIN CONTAINING 4	coord: 30..145 score: 2.2

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006626	protein targeting to mitochondrion
biological_process	GO:0006625	protein targeting to peroxisome
cellular_component	GO:0005739	mitochondrion
cellular_component	GO:0005777	peroxisome