Spo01474.1 (mRNA) | SpinachBase

Overview


Name	Spo01474.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	(Cold shock domain protein 2) (-1)
Location	SpoScf_02965 : 28336 .. 28977 (+)
Sequence length	642

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGAGCAAGGCGGAGAAAGGTTGAAGGGGTCGGTCAAGTGGTTTAATGACACCAAGGGCTTTGGTTTCATCACCCCCACTGAAGGCGGCGAAGATCTCTTCGTCCATCAGTCTTCCATCCGATCTGAGGGTTTCCGCAGCCTTGGTGACGGTGAGGAGGTTGAGTTCGTCATCTCTACCGGTGATGATGGTCGTACCAAGGCTGTTGATGTGACTGGTCCTAATGAAGGCCCTATCCAAGGCTCCACCCGTCGTTCTGGCGGTGGTAGCGGAGGAGGGGGTTACGGAGGTGGTGATGGTGGAGGATATAACGGAGGAGGAGGTGGTAGGAGATCTGGTGGAGGCGGTGGGTACGGAGGTGGAGGCGGTGGGTATGGTGGTGGTGGTGGTTATGGAGGAGGAGGCGGTGGGTATGGCGGAGGAGGTGGTGGTTACGGTGGAGGAGGCGGTGGTGGAAACTGTTATAGCTGTGGAGAGGCAGGTCACATGGCGAGGGAATGCCCATCAGGTGGTAGCGGCGGAGGTGGTGGAAGGTCGTACGGAGGTGGAGGCGGTCGTGGCGGAGGAGGTGGCGGAGGTAACTGCTACTCCTGTGGTGGTGATGGTCACTTTGCCAGGGATTGTCCCGCTGCTCGTTGA

mRNA sequence

ATGGAAGAGCAAGGCGGAGAAAGGTTGAAGGGGTCGGTCAAGTGGTTTAATGACACCAAGGGCTTTGGTTTCATCACCCCCACTGAAGGCGGCGAAGATCTCTTCGTCCATCAGTCTTCCATCCGATCTGAGGGTTTCCGCAGCCTTGGTGACGGTGAGGAGGTTGAGTTCGTCATCTCTACCGGTGATGATGGTCGTACCAAGGCTGTTGATGTGACTGGTCCTAATGAAGGCCCTATCCAAGGCTCCACCCGTCGTTCTGGCGGTGGTAGCGGAGGAGGGGGTTACGGAGGTGGTGATGGTGGAGGATATAACGGAGGAGGAGGTGGTAGGAGATCTGGTGGAGGCGGTGGGTACGGAGGTGGAGGCGGTGGGTATGGTGGTGGTGGTGGTTATGGAGGAGGAGGCGGTGGGTATGGCGGAGGAGGTGGTGGTTACGGTGGAGGAGGCGGTGGTGGAAACTGTTATAGCTGTGGAGAGGCAGGTCACATGGCGAGGGAATGCCCATCAGGTGGTAGCGGCGGAGGTGGTGGAAGGTCGTACGGAGGTGGAGGCGGTCGTGGCGGAGGAGGTGGCGGAGGTAACTGCTACTCCTGTGGTGGTGATGGTCACTTTGCCAGGGATTGTCCCGCTGCTCGTTGA

Coding sequence (CDS)

ATGGAAGAGCAAGGCGGAGAAAGGTTGAAGGGGTCGGTCAAGTGGTTTAATGACACCAAGGGCTTTGGTTTCATCACCCCCACTGAAGGCGGCGAAGATCTCTTCGTCCATCAGTCTTCCATCCGATCTGAGGGTTTCCGCAGCCTTGGTGACGGTGAGGAGGTTGAGTTCGTCATCTCTACCGGTGATGATGGTCGTACCAAGGCTGTTGATGTGACTGGTCCTAATGAAGGCCCTATCCAAGGCTCCACCCGTCGTTCTGGCGGTGGTAGCGGAGGAGGGGGTTACGGAGGTGGTGATGGTGGAGGATATAACGGAGGAGGAGGTGGTAGGAGATCTGGTGGAGGCGGTGGGTACGGAGGTGGAGGCGGTGGGTATGGTGGTGGTGGTGGTTATGGAGGAGGAGGCGGTGGGTATGGCGGAGGAGGTGGTGGTTACGGTGGAGGAGGCGGTGGTGGAAACTGTTATAGCTGTGGAGAGGCAGGTCACATGGCGAGGGAATGCCCATCAGGTGGTAGCGGCGGAGGTGGTGGAAGGTCGTACGGAGGTGGAGGCGGTCGTGGCGGAGGAGGTGGCGGAGGTAACTGCTACTCCTGTGGTGGTGATGGTCACTTTGCCAGGGATTGTCCCGCTGCTCGTTGA

Protein sequence

MEEQGGERLKGSVKWFNDTKGFGFITPTEGGEDLFVHQSSIRSEGFRSLGDGEEVEFVISTGDDGRTKAVDVTGPNEGPIQGSTRRSGGGSGGGGYGGGDGGGYNGGGGGRRSGGGGGYGGGGGGYGGGGGYGGGGGGYGGGGGGYGGGGGGGNCYSCGEAGHMARECPSGGSGGGGGRSYGGGGGRGGGGGGGNCYSCGGDGHFARDCPAAR

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo01474	Spo01474	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo01474.1	Spo01474.1-protein	polypeptide

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo01474.1.exon.1	Spo01474.1.exon.1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo01474.1.CDS.1	Spo01474.1.CDS.1	CDS

Homology

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BLAST of Spo01474.1 vs. NCBI nr
Match: gi|731357795|ref|XP_010690398.1| (PREDICTED: glycine-rich protein 2 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 255.4 bits (651), Expect = 8.900e-65
Identity = 179/214 (83.64%), Postives = 187/214 (87.38%), Query Frame = 1


		  
Query: 1   MEEQGGERLKGSVKWFNDTKGFGFITPTEGGEDLFVHQSSIRSEGFRSLGDGEEVEFVIS 60
           MEEQGGER+KG+VKWFNDTKGFGFITP++GG+DLFVHQSSIRSEGFRSLGDGEEVEFVI+
Sbjct: 1   MEEQGGERMKGTVKWFNDTKGFGFITPSDGGDDLFVHQSSIRSEGFRSLGDGEEVEFVIT 60

Query: 61  TGDDGRTKAVDVTGPNEGPIQGSTRRSGGGSGGGGYGGGDGGGYNGGGGGRRSGGGGGYG 120
           TGDDGRTKAVDVTGPNEG IQGSTRRSGGG GGGG      G Y GGGG    GGGGGYG
Sbjct: 61  TGDDGRTKAVDVTGPNEGSIQGSTRRSGGGGGGGG------GSYGGGGGSY--GGGGGYG 120

Query: 121 GGGGGYGGGGGYGGGGGGYGGG-GGGYGGGGGGGNCYSCGEAGHMARECPSGGSGGGGGR 180
           GGGG  GGGGG   GGGGYGGG GGGYGGG GGG+CYSCGE GHMARECP GGSGGGGG 
Sbjct: 121 GGGGYNGGGGGRRSGGGGYGGGSGGGYGGGSGGGSCYSCGEPGHMARECPRGGSGGGGGG 180

Query: 181 SYGGGGGRGGGGGGGNCYSCGGDGHFARDCPAAR 214
            YGGGGG GGGGGGG CYSCGG+GHFARDCPAAR
Sbjct: 181 RYGGGGGGGGGGGGGGCYSCGGEGHFARDCPAAR 206

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BLAST of Spo01474.1 vs. NCBI nr
Match: gi|902211683|gb|KNA16795.1| (hypothetical protein SOVF_086000 [Spinacia oleracea])

HSP 1 Score: 222.6 bits (566), Expect = 6.400e-55
Identity = 146/147 (99.32%), Postives = 146/147 (99.32%), Query Frame = 1


		  
Query: 1   MEEQGGERLKGSVKWFNDTKGFGFITPTEGGEDLFVHQSSIRSEGFRSLGDGEEVEFVIS 60
           MEEQGGERLKGSVKWFNDTKGFGFITPTEGGEDLFVHQSSIRSEGFRSLGDGEEVEFVIS
Sbjct: 1   MEEQGGERLKGSVKWFNDTKGFGFITPTEGGEDLFVHQSSIRSEGFRSLGDGEEVEFVIS 60

Query: 61  TGDDGRTKAVDVTGPNEGPIQGSTRRSGGGSGGGGYGGGDGGGYNGGGGGRRSGGGGGYG 120
           TGDDGRTKAVDVTGPNEGPIQGSTRRSGGGSGGGGYGGGDGGGYNGGGGGRRSGGGGGYG
Sbjct: 61  TGDDGRTKAVDVTGPNEGPIQGSTRRSGGGSGGGGYGGGDGGGYNGGGGGRRSGGGGGYG 120

Query: 121 GGGGGYGGGGGYGGGGGGYGGGGGGYG 148
           GGGGGY GGGGYGGGGGGYGGGGGGYG
Sbjct: 121 GGGGGY-GGGGYGGGGGGYGGGGGGYG 146

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BLAST of Spo01474.1 vs. NCBI nr
Match: gi|1009151133|ref|XP_015893389.1| (PREDICTED: glycine-rich protein 2-like [Ziziphus jujuba])

HSP 1 Score: 201.1 bits (510), Expect = 2.000e-48
Identity = 153/208 (73.56%), Postives = 168/208 (80.77%), Query Frame = 1


		  
Query: 7   ERLKGSVKWFNDTKGFGFITPTEGGEDLFVHQSSIRSEGFRSLGDGEEVEFVISTGDDGR 66
           ER+ G+VKWFND KGFGFITP++GGEDLFVHQSSIRSEGFRSLGDGE VEF I +  DGR
Sbjct: 3   ERVTGTVKWFNDQKGFGFITPSDGGEDLFVHQSSIRSEGFRSLGDGESVEFQIESDADGR 62

Query: 67  TKAVDVTGPNEGPIQGSTRRSGGGSGGGGYGGGDGGGYNGGGGGRRSGGGGGYGGGGGGY 126
           +KAVDVTGPNE P+QGS   SGG +GG G  GG GGGY GGG     GGGGGYGGG G  
Sbjct: 63  SKAVDVTGPNEAPVQGSRGGSGGAAGGRGSRGGGGGGYGGGGA---YGGGGGYGGGRGAR 122

Query: 127 GGGGGY--GGGGGGYGGGGGGYGGGGGGGNCYSCGEAGHMARECPSGGSGGGGGRSYGGG 186
           GGGGGY  GGGGGGYGGGGGGYGGGGGGG C+ CGE+GH+A++C  GG GGGGG  YGGG
Sbjct: 123 GGGGGYGGGGGGGGYGGGGGGYGGGGGGGACFKCGESGHIAKDCYQGGGGGGGGGRYGGG 182

Query: 187 --GGRGGGGGGGNCYSCGGDGHFARDCP 211
             GG  G GGGGNCY+CGG GHFAR+CP
Sbjct: 183 GSGGGAGAGGGGNCYNCGGSGHFARECP 207

[Close]

BLAST of Spo01474.1 vs. NCBI nr
Match: gi|645260805|ref|XP_008235991.1| (PREDICTED: glycine-rich protein 2-like [Prunus mume])

HSP 1 Score: 194.9 bits (494), Expect = 1.400e-46
Identity = 150/212 (70.75%), Postives = 162/212 (76.42%), Query Frame = 1


		  
Query: 6   GERLKGSVKWFNDTKGFGFITPTEGGEDLFVHQSSIRSEGFRSLGDGEEVEFVISTGDDG 65
           GERL G+VKWFND KGFGFITP +GG+DLFVHQSSIR+EGFR+LGDGE VEF I +  DG
Sbjct: 2   GERLSGTVKWFNDQKGFGFITPNDGGDDLFVHQSSIRTEGFRTLGDGESVEFQIESDSDG 61

Query: 66  RTKAVDVTGPNEGPIQGSTRRSGGGSGGGGYGGGDGG-GYNGGGG----GRRSGGGGGYG 125
           RTKAVDVTGP EGP+QGS  R GGG GG G GGG GG G+NGGGG    GR   GGGGY 
Sbjct: 62  RTKAVDVTGPGEGPVQGS--RGGGGGGGRGRGGGGGGYGFNGGGGRGGGGRGGRGGGGY- 121

Query: 126 GGGGGYGGGGGYGGGGGGYGGGGGGYGGGGGGGNCYSCGEAGHMARECPSGGSGG--GGG 185
                  GGGGYGGGGGGY GGGGGYGGGGGGG C+ CGE+GHMAR+C  GGSGG  GGG
Sbjct: 122 -------GGGGYGGGGGGY-GGGGGYGGGGGGGACFKCGESGHMARDCEQGGSGGGYGGG 181

Query: 186 RSYGGGGGRGGGGGGGNCYSCGGDGHFARDCP 211
             Y GGG  GGGG  G CY CG  GHFAR+CP
Sbjct: 182 GGYSGGGRGGGGGSSGGCYGCGESGHFARECP 202

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BLAST of Spo01474.1 vs. NCBI nr
Match: gi|1021052719|gb|KZN10497.1| (hypothetical protein DCAR_003153 [Daucus carota subsp. sativus])

HSP 1 Score: 193.0 bits (489), Expect = 5.500e-46
Identity = 142/223 (63.68%), Postives = 150/223 (67.26%), Query Frame = 1


		  
Query: 4   QGGERLKGSVKWFNDTKGFGFITPTEGGEDLFVHQSSIRSEGFRSLGDGEEVEFVISTGD 63
           + G RLKGSVKWFNDTKGFGFITP EGGEDLFVHQSSI+S+GFRSL +GE VEFVI  G 
Sbjct: 3   EDGSRLKGSVKWFNDTKGFGFITPDEGGEDLFVHQSSIKSDGFRSLAEGESVEFVIENGS 62

Query: 64  DGRTKAVDVTGPNEGPIQGSTRRSGGGSG--GGGYGGGDGGGYNGGGGGRRSGGGGGYGG 123
           DGRTKAVDVTGP+   +QGS R  GGG G  GGGYGGG GG               GYGG
Sbjct: 63  DGRTKAVDVTGPDGASVQGSGRGGGGGGGRGGGGYGGG-GG---------------GYGG 122

Query: 124 GGGGYGGGGGYGGGGGGYGGGGGGYGGGGGGGNCYSCGEAGHMARECPSGGSG---GGGG 183
           GGGGYGGGGGY               GGGGGG CY CGE GHMAR+C +GG G   GGGG
Sbjct: 123 GGGGYGGGGGY---------------GGGGGGGCYKCGENGHMARDCDNGGGGGGYGGGG 182

Query: 184 RSYGGGGGRG-----------GGGGGGNCYSCGGDGHFARDCP 211
             YGGGGG G           GGGGGG CY CG DGHFARDCP
Sbjct: 183 GGYGGGGGYGGGRGGGRGGGRGGGGGGGCYQCGEDGHFARDCP 194

[Close]

BLAST of Spo01474.1 vs. UniProtKB/TrEMBL
Match: A0A0J8EEM7_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_9g214350 PE=4 SV=1)

HSP 1 Score: 255.4 bits (651), Expect = 6.200e-65
Identity = 179/214 (83.64%), Postives = 187/214 (87.38%), Query Frame = 1


		  
Query: 1   MEEQGGERLKGSVKWFNDTKGFGFITPTEGGEDLFVHQSSIRSEGFRSLGDGEEVEFVIS 60
           MEEQGGER+KG+VKWFNDTKGFGFITP++GG+DLFVHQSSIRSEGFRSLGDGEEVEFVI+
Sbjct: 1   MEEQGGERMKGTVKWFNDTKGFGFITPSDGGDDLFVHQSSIRSEGFRSLGDGEEVEFVIT 60

Query: 61  TGDDGRTKAVDVTGPNEGPIQGSTRRSGGGSGGGGYGGGDGGGYNGGGGGRRSGGGGGYG 120
           TGDDGRTKAVDVTGPNEG IQGSTRRSGGG GGGG      G Y GGGG    GGGGGYG
Sbjct: 61  TGDDGRTKAVDVTGPNEGSIQGSTRRSGGGGGGGG------GSYGGGGGSY--GGGGGYG 120

Query: 121 GGGGGYGGGGGYGGGGGGYGGG-GGGYGGGGGGGNCYSCGEAGHMARECPSGGSGGGGGR 180
           GGGG  GGGGG   GGGGYGGG GGGYGGG GGG+CYSCGE GHMARECP GGSGGGGG 
Sbjct: 121 GGGGYNGGGGGRRSGGGGYGGGSGGGYGGGSGGGSCYSCGEPGHMARECPRGGSGGGGGG 180

Query: 181 SYGGGGGRGGGGGGGNCYSCGGDGHFARDCPAAR 214
            YGGGGG GGGGGGG CYSCGG+GHFARDCPAAR
Sbjct: 181 RYGGGGGGGGGGGGGGCYSCGGEGHFARDCPAAR 206

[Close]

BLAST of Spo01474.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RBD3_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_086000 PE=4 SV=1)

HSP 1 Score: 222.6 bits (566), Expect = 4.500e-55
Identity = 146/147 (99.32%), Postives = 146/147 (99.32%), Query Frame = 1


		  
Query: 1   MEEQGGERLKGSVKWFNDTKGFGFITPTEGGEDLFVHQSSIRSEGFRSLGDGEEVEFVIS 60
           MEEQGGERLKGSVKWFNDTKGFGFITPTEGGEDLFVHQSSIRSEGFRSLGDGEEVEFVIS
Sbjct: 1   MEEQGGERLKGSVKWFNDTKGFGFITPTEGGEDLFVHQSSIRSEGFRSLGDGEEVEFVIS 60

Query: 61  TGDDGRTKAVDVTGPNEGPIQGSTRRSGGGSGGGGYGGGDGGGYNGGGGGRRSGGGGGYG 120
           TGDDGRTKAVDVTGPNEGPIQGSTRRSGGGSGGGGYGGGDGGGYNGGGGGRRSGGGGGYG
Sbjct: 61  TGDDGRTKAVDVTGPNEGPIQGSTRRSGGGSGGGGYGGGDGGGYNGGGGGRRSGGGGGYG 120

Query: 121 GGGGGYGGGGGYGGGGGGYGGGGGGYG 148
           GGGGGY GGGGYGGGGGGYGGGGGGYG
Sbjct: 121 GGGGGY-GGGGYGGGGGGYGGGGGGYG 146

[Close]

BLAST of Spo01474.1 vs. UniProtKB/TrEMBL
Match: A0A0K9P8C9_ZOSMR (Cold shock domain-containing protein 4 OS=Zostera marina GN=ZOSMA_32G00500 PE=4 SV=1)

HSP 1 Score: 188.3 bits (477), Expect = 9.400e-45
Identity = 151/213 (70.89%), Postives = 164/213 (77.00%), Query Frame = 1


		  
Query: 8   RLKGSVKWFNDTKGFGFITPTEGGEDLFVHQSSIRSEGFRSLGDGEEVEFVISTGDDGRT 67
           R KG VKWFN TKGFGFITP +G EDLFVHQSSI+S+G+RSL +GE VEFV+S GDDGRT
Sbjct: 5   RSKGIVKWFNGTKGFGFITPEDGSEDLFVHQSSIKSDGYRSLAEGESVEFVVSAGDDGRT 64

Query: 68  KAVDVTGPNEGPIQGSTRRSGGGSG---GGGYGGGDGGGYNGGGGGRRSGGGGGYGGGGG 127
           KAVDVTGP+   +QG   R GGG G   GGGYGGG GGGY GGGG R   GGGG  GGGG
Sbjct: 65  KAVDVTGPDGASVQGDGGRGGGGGGYGGGGGYGGG-GGGYGGGGGYR--SGGGGRNGGGG 124

Query: 128 GYGGGGGYGGGGGGYGGGGGGYGGGGGGGNCYSCGEAGHMARECPSGGSGGGGGRSYGGG 187
           GYGGGGGYG      GGGGGGYGGGGGGG CY+CGE GHMARECP+GG GGGG    GGG
Sbjct: 125 GYGGGGGYG------GGGGGGYGGGGGGGGCYNCGEQGHMARECPTGG-GGGGRYGGGGG 184

Query: 188 GGR----GGGGGGGNCYSCGGDGHFARDCPAAR 214
           GGR    GGGGGGG CY+CG  GHFAR+C A +
Sbjct: 185 GGRYGGGGGGGGGGACYNCGEPGHFARECSAMK 207

[Close]

BLAST of Spo01474.1 vs. UniProtKB/TrEMBL
Match: F6I111_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_03s0038g02130 PE=4 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 1.400e-43
Identity = 151/213 (70.89%), Postives = 164/213 (77.00%), Query Frame = 1


		  
Query: 8   RLKGSVKWFNDTKGFGFITPTEGGEDLFVHQSSIRSEGFRSLGDGEEVEFVISTGDDGRT 67
           R+ G+VKWFND KGFGFITP +GG+DLFVHQSSIRSEGFRSLG+GE VEFV+ + DDGRT
Sbjct: 3   RVTGTVKWFNDQKGFGFITPDDGGDDLFVHQSSIRSEGFRSLGEGEAVEFVVESSDDGRT 62

Query: 68  KAVDVTGPNEGPIQGSTRRSGG--GSGGGGYGGGDGGGYNGGGGGRRSGGGGGYGGGGGG 127
           KAVDVTGP+  P+QGS    GG  G GGGGYGGG  GGY GGGGG    GGGG GGG GG
Sbjct: 63  KAVDVTGPDGNPVQGSRGGGGGRGGRGGGGYGGG--GGYGGGGGGYGYNGGGGRGGGRGG 122

Query: 128 YGGGGG------YGGGGGGYGGGGGGYGGGGGGGNCYSCGEAGHMAREC--PSGGSGGGG 187
            GGGGG      YGGGG    GGGGGYGGGGGGG CY+CGE GH+AR+C   SGG GGGG
Sbjct: 123 RGGGGGGRSSGGYGGGG---YGGGGGYGGGGGGGACYNCGEEGHLARDCSQSSGGGGGGG 182

Query: 188 GRSYGGGGGRGGGGGGGNCYSCGGDGHFARDCP 211
             S GGGGG GGGGGGG CY CG  GHFAR+CP
Sbjct: 183 RYSGGGGGGGGGGGGGGGCYRCGEAGHFARECP 210

[Close]

BLAST of Spo01474.1 vs. UniProtKB/TrEMBL
Match: A5BQ96_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_040408 PE=4 SV=1)

HSP 1 Score: 183.3 bits (464), Expect = 3.000e-43
Identity = 148/225 (65.78%), Postives = 161/225 (71.56%), Query Frame = 1


		  
Query: 8   RLKGSVKWFNDTKGFGFITPTEGGEDLFVHQSSIRSEGFRSLGDGEEVEFVISTGDDGRT 67
           R+ G+VKWFND KGFGFITP +GG+DLFVHQSSIRSEGFRSLG+GE VEFV+ + DDGRT
Sbjct: 3   RVTGTVKWFNDQKGFGFITPDDGGDDLFVHQSSIRSEGFRSLGEGEAVEFVVESSDDGRT 62

Query: 68  KAVDVTGPNEGPIQGSTRRSGG-----------GSGGGGYGGGDGG-GYNGGGG--GRRS 127
           KAVDVTGP+  P+QGS    GG           G GGGGYGGG GG GYNGGGG  G R 
Sbjct: 63  KAVDVTGPDGNPVQGSRGGGGGRGGRGGGGYGGGGGGGGYGGGGGGYGYNGGGGRGGGRG 122

Query: 128 GGGGGYGG------GGGGYGGGGGYGGGGGGYGGGGGGYGGGGGGGNCYSCGEAGHMARE 187
           G GGG GG      GGGGYGGGGGYGG            GGGGGGG CY+CGE GH+AR+
Sbjct: 123 GRGGGGGGRSSGGYGGGGYGGGGGYGG------------GGGGGGGACYNCGEEGHLARD 182

Query: 188 C--PSGGSGGGGGRSYGGGGGRGGGGGGGNCYSCGGDGHFARDCP 211
           C   SGG GGGG  S GGGGG GGGGGGG CY CG  GHFAR+CP
Sbjct: 183 CSQSSGGGGGGGRYSSGGGGGGGGGGGGGGCYRCGEAGHFARECP 215

[Close]

BLAST of Spo01474.1 vs. ExPASy Swiss-Prot
Match: GRP2_NICSY (Glycine-rich protein 2 OS=Nicotiana sylvestris GN=GRP-2 PE=2 SV=1)

HSP 1 Score: 174.5 bits (441), Expect = 1.300e-42
Identity = 145/213 (68.08%), Postives = 160/213 (75.12%), Query Frame = 1


		  
Query: 1   MEEQGGERLKGSVKWFNDTKGFGFITPTEGGEDLFVHQSSIRSEGFRSLGDGEEVEFVIS 60
           M E+ G+R KG+VKWF+D KGFGFITP +GGEDLFVHQS IRSEGFRSL +GE VEF + 
Sbjct: 1   MAEESGQRAKGTVKWFSDQKGFGFITPDDGGEDLFVHQSGIRSEGFRSLAEGETVEFEVE 60

Query: 61  TGDDGRTKAVDVTGPNEGPIQGSTRRSGGGS--GGGGYGGGDGG-GYNGGGGGRRSGGGG 120
           +G DGRTKAVDVTGP+   +QG     GGG   GGGGYGGG GG G  G GG R  GGG 
Sbjct: 61  SGGDGRTKAVDVTGPDGAAVQGGRGGGGGGGGRGGGGYGGGSGGYGGGGRGGSRGYGGGD 120

Query: 121 GYGGGGGGYGGGGGYGGGGGGYGGGGG-GYGGGGGGGNCYSCGEAGHMARECPSGGSGGG 180
           G  GGGGGYGGG  YGGGGGGYGGGGG G GG GGG  C+ CGE+GH AR+C   G GGG
Sbjct: 121 GGYGGGGGYGGGSRYGGGGGGYGGGGGYGGGGSGGGSGCFKCGESGHFARDCSQSGGGGG 180

Query: 181 GGRSYGGGGGRGGGGGGGNCYSCGGDGHFARDC 210
           GGR +GGG   GGGGGGG CY CG DGHFAR+C
Sbjct: 181 GGR-FGGG---GGGGGGGGCYKCGEDGHFAREC 209

[Close]

BLAST of Spo01474.1 vs. ExPASy Swiss-Prot
Match: CSP2_ARATH (Cold shock protein 2 OS=Arabidopsis thaliana GN=CSP2 PE=1 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 2.300e-36
Identity = 132/219 (60.27%), Postives = 147/219 (67.12%), Query Frame = 1


		  
Query: 5   GGERLKGSVKWFNDTKGFGFITPTEGGEDLFVHQSSIRSEGFRSLGDGEEVEFVISTGDD 64
           GGER KGSVKWF+  KGFGFITP +GG+DLFVHQSSIRSEGFRSL   E VEF +   ++
Sbjct: 7   GGERRKGSVKWFDTQKGFGFITPDDGGDDLFVHQSSIRSEGFRSLAAEEAVEFEVEIDNN 66

Query: 65  GRTKAVDVTGPNEGPIQGSTRRSGGGSGGGGYGGGDGGGYNGGGGGRRSGGGGGYGGG-G 124
            R KA+DV+GP+  P+QG+   SGGGS                     SGG GG+GGG G
Sbjct: 67  NRPKAIDVSGPDGAPVQGN---SGGGS---------------------SGGRGGFGGGRG 126

Query: 125 GGYGGGGGYGGGGGGYGGGGGGYGGGGGGGNCYSCGEAGHMARECPSGGSG-GGGGRSYG 184
           GG G GGGYGGGGGGYGG G   GGG GG +CY CGE GHMAR+C  GG G GGGG  YG
Sbjct: 127 GGRGSGGGYGGGGGGYGGRG---GGGRGGSDCYKCGEPGHMARDCSEGGGGYGGGGGGYG 186

Query: 185 ------------GGGGRGGGGGGGNCYSCGGDGHFARDC 210
                       GGGGRGGGGGGG+CYSCG  GHFARDC
Sbjct: 187 GGGGYGGGGGGYGGGGRGGGGGGGSCYSCGESGHFARDC 198

[Close]

BLAST of Spo01474.1 vs. ExPASy Swiss-Prot
Match: CSP4_ARATH (Cold shock domain-containing protein 4 OS=Arabidopsis thaliana GN=CSP4 PE=1 SV=1)

HSP 1 Score: 145.2 bits (365), Expect = 8.200e-34
Identity = 123/209 (58.85%), Postives = 140/209 (66.99%), Query Frame = 1


		  
Query: 5   GGERLKGSVKWFNDTKGFGFITPTEGGEDLFVHQSSIRSEGFRSLGDGEEVEFVISTGDD 64
           GG+R KG+VKWF+  KGFGFITP++GG+DLFVHQSSIRSEGFRSL   E VEF +   + 
Sbjct: 11  GGDRRKGTVKWFDTQKGFGFITPSDGGDDLFVHQSSIRSEGFRSLAAEESVEFDVEVDNS 70

Query: 65  GRTKAVDVTGPNEGPIQGSTRRSGGGSGGGGYGGGDGGGYNGGGGGRRSGG-GGGYGGGG 124
           GR KA++V+GP+  P+QG+   SGGG   GG GG  GGG  G GGGR  G  GGGYG   
Sbjct: 71  GRPKAIEVSGPDGAPVQGN---SGGGGSSGGRGGFGGGG--GRGGGRGGGSYGGGYG--- 130

Query: 125 GGYGGGGGYGGGGGGYGGGGGGYGGGGGGGNCYSCGEAGHMARECPSGG---SGGGGGRS 184
                           G G GG GGGGG  +C+ CGE GHMAREC  GG   SGGGGG  
Sbjct: 131 ----------------GRGSGGRGGGGGDNSCFKCGEPGHMARECSQGGGGYSGGGGGGR 190

Query: 185 YGGGGGRGGGGGGGNCYSCGGDGHFARDC 210
           YG GGG GGGGGG +CYSCG  GHFARDC
Sbjct: 191 YGSGGGGGGGGGGLSCYSCGESGHFARDC 195

[Close]

BLAST of Spo01474.1 vs. ExPASy Swiss-Prot
Match: CSP3_ARATH (Cold shock domain-containing protein 3 OS=Arabidopsis thaliana GN=CSP3 PE=1 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 2.500e-19
Identity = 105/237 (44.30%), Postives = 123/237 (51.90%), Query Frame = 1


		  
Query: 1   MEEQGGERLKGSVKWFNDTKGFGFITPTEGGEDLFVHQSSIRSEGFRSLGDGEEVEFVIS 60
           ME+Q   R  G V WF+D KG+GFITP +GGE+LFVHQSSI S+GFRSL  GE VE+ I+
Sbjct: 3   MEDQSAARSIGKVSWFSDGKGYGFITPDDGGEELFVHQSSIVSDGFRSLTLGESVEYEIA 62

Query: 61  TGDDGRTKAVDVTGPNEGPI---QGSTRRSGGGSGGGGYGGGDGGGYNGGGGGRRSGGGG 120
            G DG+TKA++VT P  G +   + S+R SGG     G  G      +GG GG+  GGGG
Sbjct: 63  LGSDGKTKAIEVTAPGGGSLNKKENSSRGSGGNCFNCGEVGHMAKDCDGGSGGKSFGGGG 122

Query: 121 GYGGGGGGYGGGGGYGGGGGGYGGGGGGYGGGGGGGNCYSCGEAGHMAREC------PSG 180
           G                               GG G CY CG+ GH AR+C       SG
Sbjct: 123 G----------------------------RRSGGEGECYMCGDVGHFARDCRQSGGGNSG 182

Query: 181 GSGGGGGRSY----------------GG---GGGRGGGGGGGNCYSCGGDGHFARDC 210
           G GGGG   Y                GG   GGG G G GG  CY CGG GHFARDC
Sbjct: 183 GGGGGGRPCYSCGEVGHLAKDCRGGSGGNRYGGGGGRGSGGDGCYMCGGVGHFARDC 211

[Close]

BLAST of Spo01474.1 vs. ExPASy Swiss-Prot
Match: CSP1_ARATH (Cold shock protein 1 OS=Arabidopsis thaliana GN=CSP1 PE=1 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 6.100e-13
Identity = 91/208 (43.75%), Postives = 112/208 (53.85%), Query Frame = 1


		  
Query: 2   EEQGGERLKGSVKWFNDTKGFGFITPTEGGEDLFVHQSSIRSEGFRSLGDGEEVEFVIST 61
           E+Q   R  G V WFN +KG+GFITP +G  +LFVHQSSI SEG+RSL  G+ VEF I+ 
Sbjct: 4   EDQSAARSTGKVNWFNASKGYGFITPDDGSVELFVHQSSIVSEGYRSLTVGDAVEFAITQ 63

Query: 62  GDDGRTKAVDVTGPNEGPIQGSTRRSGGGSGGGGYGGGDGGGYNGGGGGRRSGGGGGYGG 121
           G DG+TKAV+VT P  G ++      G G+  GG           GG G  + G  G+  
Sbjct: 64  GSDGKTKAVNVTAPGGGSLKKENNSRGNGARRGG-----------GGSGCYNCGELGHIS 123

Query: 122 GGGGYGGGGGYGGGGGGYGGGGGGYGGGGGGGNCYSCGEAGHMARECPSGGSGGGGGRSY 181
              G GGGGG            GG     GG  CY+CG+ GH AR+C S G+G   G + 
Sbjct: 124 KDCGIGGGGG------------GGERRSRGGEGCYNCGDTGHFARDCTSAGNGDQRGATK 179

Query: 182 GGGGGRGGGGGGGNCYSCGGDGHFARDC 210
           GG  G         CY+CG  GH ARDC
Sbjct: 184 GGNDG---------CYTCGDVGHVARDC 179

[Close]

BLAST of Spo01474.1 vs. TAIR (Arabidopsis)
Match: AT4G38680.1 (glycine rich protein 2)

HSP 1 Score: 153.7 bits (387), Expect = 1.300e-37
Identity = 132/219 (60.27%), Postives = 147/219 (67.12%), Query Frame = 1


		  
Query: 5   GGERLKGSVKWFNDTKGFGFITPTEGGEDLFVHQSSIRSEGFRSLGDGEEVEFVISTGDD 64
           GGER KGSVKWF+  KGFGFITP +GG+DLFVHQSSIRSEGFRSL   E VEF +   ++
Sbjct: 7   GGERRKGSVKWFDTQKGFGFITPDDGGDDLFVHQSSIRSEGFRSLAAEEAVEFEVEIDNN 66

Query: 65  GRTKAVDVTGPNEGPIQGSTRRSGGGSGGGGYGGGDGGGYNGGGGGRRSGGGGGYGGG-G 124
            R KA+DV+GP+  P+QG+   SGGGS                     SGG GG+GGG G
Sbjct: 67  NRPKAIDVSGPDGAPVQGN---SGGGS---------------------SGGRGGFGGGRG 126

Query: 125 GGYGGGGGYGGGGGGYGGGGGGYGGGGGGGNCYSCGEAGHMARECPSGGSG-GGGGRSYG 184
           GG G GGGYGGGGGGYGG G   GGG GG +CY CGE GHMAR+C  GG G GGGG  YG
Sbjct: 127 GGRGSGGGYGGGGGGYGGRG---GGGRGGSDCYKCGEPGHMARDCSEGGGGYGGGGGGYG 186

Query: 185 ------------GGGGRGGGGGGGNCYSCGGDGHFARDC 210
                       GGGGRGGGGGGG+CYSCG  GHFARDC
Sbjct: 187 GGGGYGGGGGGYGGGGRGGGGGGGSCYSCGESGHFARDC 198

[Close]

BLAST of Spo01474.1 vs. TAIR (Arabidopsis)
Match: AT2G21060.1 (glycine-rich protein 2B)

HSP 1 Score: 145.2 bits (365), Expect = 4.600e-35
Identity = 123/209 (58.85%), Postives = 140/209 (66.99%), Query Frame = 1


		  
Query: 5   GGERLKGSVKWFNDTKGFGFITPTEGGEDLFVHQSSIRSEGFRSLGDGEEVEFVISTGDD 64
           GG+R KG+VKWF+  KGFGFITP++GG+DLFVHQSSIRSEGFRSL   E VEF +   + 
Sbjct: 11  GGDRRKGTVKWFDTQKGFGFITPSDGGDDLFVHQSSIRSEGFRSLAAEESVEFDVEVDNS 70

Query: 65  GRTKAVDVTGPNEGPIQGSTRRSGGGSGGGGYGGGDGGGYNGGGGGRRSGG-GGGYGGGG 124
           GR KA++V+GP+  P+QG+   SGGG   GG GG  GGG  G GGGR  G  GGGYG   
Sbjct: 71  GRPKAIEVSGPDGAPVQGN---SGGGGSSGGRGGFGGGG--GRGGGRGGGSYGGGYG--- 130

Query: 125 GGYGGGGGYGGGGGGYGGGGGGYGGGGGGGNCYSCGEAGHMARECPSGG---SGGGGGRS 184
                           G G GG GGGGG  +C+ CGE GHMAREC  GG   SGGGGG  
Sbjct: 131 ----------------GRGSGGRGGGGGDNSCFKCGEPGHMARECSQGGGGYSGGGGGGR 190

Query: 185 YGGGGGRGGGGGGGNCYSCGGDGHFARDC 210
           YG GGG GGGGGG +CYSCG  GHFARDC
Sbjct: 191 YGSGGGGGGGGGGLSCYSCGESGHFARDC 195

[Close]

BLAST of Spo01474.1 vs. TAIR (Arabidopsis)
Match: AT2G17870.1 (cold shock domain protein 3)

HSP 1 Score: 97.1 bits (240), Expect = 1.400e-20
Identity = 105/237 (44.30%), Postives = 123/237 (51.90%), Query Frame = 1


		  
Query: 1   MEEQGGERLKGSVKWFNDTKGFGFITPTEGGEDLFVHQSSIRSEGFRSLGDGEEVEFVIS 60
           ME+Q   R  G V WF+D KG+GFITP +GGE+LFVHQSSI S+GFRSL  GE VE+ I+
Sbjct: 3   MEDQSAARSIGKVSWFSDGKGYGFITPDDGGEELFVHQSSIVSDGFRSLTLGESVEYEIA 62

Query: 61  TGDDGRTKAVDVTGPNEGPI---QGSTRRSGGGSGGGGYGGGDGGGYNGGGGGRRSGGGG 120
            G DG+TKA++VT P  G +   + S+R SGG     G  G      +GG GG+  GGGG
Sbjct: 63  LGSDGKTKAIEVTAPGGGSLNKKENSSRGSGGNCFNCGEVGHMAKDCDGGSGGKSFGGGG 122

Query: 121 GYGGGGGGYGGGGGYGGGGGGYGGGGGGYGGGGGGGNCYSCGEAGHMAREC------PSG 180
           G                               GG G CY CG+ GH AR+C       SG
Sbjct: 123 G----------------------------RRSGGEGECYMCGDVGHFARDCRQSGGGNSG 182

Query: 181 GSGGGGGRSY----------------GG---GGGRGGGGGGGNCYSCGGDGHFARDC 210
           G GGGG   Y                GG   GGG G G GG  CY CGG GHFARDC
Sbjct: 183 GGGGGGRPCYSCGEVGHLAKDCRGGSGGNRYGGGGGRGSGGDGCYMCGGVGHFARDC 211

[Close]

BLAST of Spo01474.1 vs. TAIR (Arabidopsis)
Match: AT4G36020.1 (cold shock domain protein 1)

HSP 1 Score: 75.9 bits (185), Expect = 3.400e-14
Identity = 91/208 (43.75%), Postives = 112/208 (53.85%), Query Frame = 1


		  
Query: 2   EEQGGERLKGSVKWFNDTKGFGFITPTEGGEDLFVHQSSIRSEGFRSLGDGEEVEFVIST 61
           E+Q   R  G V WFN +KG+GFITP +G  +LFVHQSSI SEG+RSL  G+ VEF I+ 
Sbjct: 4   EDQSAARSTGKVNWFNASKGYGFITPDDGSVELFVHQSSIVSEGYRSLTVGDAVEFAITQ 63

Query: 62  GDDGRTKAVDVTGPNEGPIQGSTRRSGGGSGGGGYGGGDGGGYNGGGGGRRSGGGGGYGG 121
           G DG+TKAV+VT P  G ++      G G+  GG           GG G  + G  G+  
Sbjct: 64  GSDGKTKAVNVTAPGGGSLKKENNSRGNGARRGG-----------GGSGCYNCGELGHIS 123

Query: 122 GGGGYGGGGGYGGGGGGYGGGGGGYGGGGGGGNCYSCGEAGHMARECPSGGSGGGGGRSY 181
              G GGGGG            GG     GG  CY+CG+ GH AR+C S G+G   G + 
Sbjct: 124 KDCGIGGGGG------------GGERRSRGGEGCYNCGDTGHFARDCTSAGNGDQRGATK 179

Query: 182 GGGGGRGGGGGGGNCYSCGGDGHFARDC 210
           GG  G         CY+CG  GH ARDC
Sbjct: 184 GGNDG---------CYTCGDVGHVARDC 179

The following BLAST results are available for this feature:

BLAST of Spo01474.1 vs. NCBI nr
BLAST of Spo01474.1 vs. UniProtKB/TrEMBL
BLAST of Spo01474.1 vs. ExPASy Swiss-Prot
BLAST of Spo01474.1 vs. TAIR (Arabidopsis)

BLAST of Spo01474.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|731357795\|ref\|XP_010690398.1\|	8.9e-65	83.6	PREDICTED: glycine-rich protei...	[more]
gi\|902211683\|gb\|KNA16795.1\|	6.4e-55	99.3	hypothetical protein SOVF_0860...	[more]
gi\|1009151133\|ref\|XP_015893389.1\|	2.0e-48	73.5	PREDICTED: glycine-rich protei...	[more]
gi\|645260805\|ref\|XP_008235991.1\|	1.4e-46	70.7	PREDICTED: glycine-rich protei...	[more]
gi\|1021052719\|gb\|KZN10497.1\|	5.5e-46	63.6	hypothetical protein DCAR_0031...	[more]

BLAST of Spo01474.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0J8EEM7_BETVU	6.2e-65	83.6	Uncharacterized protein OS=Bet...	[more]
A0A0K9RBD3_SPIOL	4.5e-55	99.3	Uncharacterized protein OS=Spi...	[more]
A0A0K9P8C9_ZOSMR	9.4e-45	70.8	Cold shock domain-containing p...	[more]
F6I111_VITVI	1.4e-43	70.8	Putative uncharacterized prote...	[more]
A5BQ96_VITVI	3.0e-43	65.7	Putative uncharacterized prote...	[more]

BLAST of Spo01474.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
GRP2_NICSY	1.3e-42	68.0	Glycine-rich protein 2 OS=Nico...	[more]
CSP2_ARATH	2.3e-36	60.2	Cold shock protein 2 OS=Arabid...	[more]
CSP4_ARATH	8.2e-34	58.8	Cold shock domain-containing p...	[more]
CSP3_ARATH	2.5e-19	44.3	Cold shock domain-containing p...	[more]
CSP1_ARATH	6.1e-13	43.7	Cold shock protein 1 OS=Arabid...	[more]

BLAST of Spo01474.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 4


Match Name	E-value	Identity	Description
AT4G38680.1	1.3e-37	60.2	glycine rich protein 2	[more]
AT2G21060.1	4.6e-35	58.8	glycine-rich protein 2B	[more]
AT2G17870.1	1.4e-20	44.3	cold shock domain protein 3	[more]
AT4G36020.1	3.4e-14	43.7	cold shock domain protein 1	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001878	Zinc finger, CCHC-type	GENE3D	4.10.60.10		coord: 153..211 score: 1.3
IPR001878	Zinc finger, CCHC-type	PFAM	PF00098	zf-CCHC	coord: 154..170 score: 1.8E-7coord: 195..210 score: 2.
IPR001878	Zinc finger, CCHC-type	SMART	SM00343	c2hcfinal6	coord: 195..211 score: 1.2E-4coord: 154..170 score: 1.
IPR001878	Zinc finger, CCHC-type	PROFILE	PS50158	ZF_CCHC	coord: 155..170 score: 11.334coord: 196..210 score: 10
IPR001878	Zinc finger, CCHC-type	unknown	SSF57756	Retrovirus zinc finger-like domains	coord: 145..212 score: 6.0
IPR002059	Cold-shock protein, DNA-binding	PRINTS	PR00050	COLDSHOCK	coord: 11..26 score: 1.3E-15coord: 32..41 score: 1.3E-15coord: 47..65 score: 1.3
IPR002059	Cold-shock protein, DNA-binding	PFAM	PF00313	CSD	coord: 10..74 score: 2.7
IPR011129	Cold shock protein	SMART	SM00357	csp_8	coord: 10..75 score: 1.6
IPR012340	Nucleic acid-binding, OB-fold	GENE3D	2.40.50.140		coord: 10..73 score: 2.6
IPR012340	Nucleic acid-binding, OB-fold	unknown	SSF50249	Nucleic acid-binding proteins	coord: 7..74 score: 7.07
IPR019844	Cold-shock conserved site	PROSITE	PS00352	COLD_SHOCK	coord: 22..41 scor
None	No IPR available	PANTHER	PTHR11544	COLD SHOCK DOMAIN CONTAINING PROTEINS	coord: 1..210 score: 2.4

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006355	regulation of transcription, DNA-templated
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0008270	zinc ion binding