Spo02971.1 (mRNA) | SpinachBase

Overview


Name	Spo02971.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	Mitochondrial import inner membrane translocase subunit Tim17 family protein
Location	SpoScf_03075 : 17991 .. 18692 (+)
Sequence length	702

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAACACCAGAAACATCTCGAGAACCGTGTCCTGACCGTATCCTCGACGACTTAGGCGGCGCCTTTGCCATGGGAGCTATCGGAGGTTCTGTCTTCCACTACGTCAGGGGTTCAATCAACGCCCCTAAAGGTGAACGCATCCTTCGAGCGCTCGAAACTGTGCGAATGAGTGCGCCGCGTACTGGTGGTGGGTTCGCCGTATGGGGTGGTTTGTTCTCCACTTTTGACTGTACTATGGTCTACATCCGCCAAAAGGAGGATCCTTGGAATTCTATTTTCGCCGGCGCGGCTACCGGTGGGTTCCTTCAGGTGCGTCAGGGTCTACGCGCCTCCGGTCGTTCTGCCGTATTTGGTGGGATCCTTCTTGCTTTGATTGAAGGTGCTGGGATCATGCTTAATCGGGTTGTGTCGCCGCCTGCACAGATTATGTATGAGGATCCGAGTATGGGTGCTGGGGCCCCGCAGATGGGGGGTTATGGGTTTGGGTTTCCGGGTCAGGCGAGGGCACCTGCGAACGTTGGTGGTGGTGGGTCCGAGGAGGTGAATCAAGGTGGTAGTGGTGGTGGTGGGTTTTTTGGTGGGTGGTTTGGTGGTGATGGGAAGAAAGAAGGGAGTAGTTCAAGTAGCGGTGGGAGCGAAGCGACAGTTTTGGAGAGCTTTGATACGCCTACTCCTATGCCTAATTTTGATTACAAATGA

mRNA sequence

ATGGGAACACCAGAAACATCTCGAGAACCGTGTCCTGACCGTATCCTCGACGACTTAGGCGGCGCCTTTGCCATGGGAGCTATCGGAGGTTCTGTCTTCCACTACGTCAGGGGTTCAATCAACGCCCCTAAAGGTGAACGCATCCTTCGAGCGCTCGAAACTGTGCGAATGAGTGCGCCGCGTACTGGTGGTGGGTTCGCCGTATGGGGTGGTTTGTTCTCCACTTTTGACTGTACTATGGTCTACATCCGCCAAAAGGAGGATCCTTGGAATTCTATTTTCGCCGGCGCGGCTACCGGTGGGTTCCTTCAGGTGCGTCAGGGTCTACGCGCCTCCGGTCGTTCTGCCGTATTTGGTGGGATCCTTCTTGCTTTGATTGAAGGTGCTGGGATCATGCTTAATCGGGTTGTGTCGCCGCCTGCACAGATTATGTATGAGGATCCGAGTATGGGTGCTGGGGCCCCGCAGATGGGGGGTTATGGGTTTGGGTTTCCGGGTCAGGCGAGGGCACCTGCGAACGTTGGTGGTGGTGGGTCCGAGGAGGTGAATCAAGGTGGTAGTGGTGGTGGTGGGTTTTTTGGTGGGTGGTTTGGTGGTGATGGGAAGAAAGAAGGGAGTAGTTCAAGTAGCGGTGGGAGCGAAGCGACAGTTTTGGAGAGCTTTGATACGCCTACTCCTATGCCTAATTTTGATTACAAATGA

Coding sequence (CDS)

ATGGGAACACCAGAAACATCTCGAGAACCGTGTCCTGACCGTATCCTCGACGACTTAGGCGGCGCCTTTGCCATGGGAGCTATCGGAGGTTCTGTCTTCCACTACGTCAGGGGTTCAATCAACGCCCCTAAAGGTGAACGCATCCTTCGAGCGCTCGAAACTGTGCGAATGAGTGCGCCGCGTACTGGTGGTGGGTTCGCCGTATGGGGTGGTTTGTTCTCCACTTTTGACTGTACTATGGTCTACATCCGCCAAAAGGAGGATCCTTGGAATTCTATTTTCGCCGGCGCGGCTACCGGTGGGTTCCTTCAGGTGCGTCAGGGTCTACGCGCCTCCGGTCGTTCTGCCGTATTTGGTGGGATCCTTCTTGCTTTGATTGAAGGTGCTGGGATCATGCTTAATCGGGTTGTGTCGCCGCCTGCACAGATTATGTATGAGGATCCGAGTATGGGTGCTGGGGCCCCGCAGATGGGGGGTTATGGGTTTGGGTTTCCGGGTCAGGCGAGGGCACCTGCGAACGTTGGTGGTGGTGGGTCCGAGGAGGTGAATCAAGGTGGTAGTGGTGGTGGTGGGTTTTTTGGTGGGTGGTTTGGTGGTGATGGGAAGAAAGAAGGGAGTAGTTCAAGTAGCGGTGGGAGCGAAGCGACAGTTTTGGAGAGCTTTGATACGCCTACTCCTATGCCTAATTTTGATTACAAATGA

Protein sequence

MGTPETSREPCPDRILDDLGGAFAMGAIGGSVFHYVRGSINAPKGERILRALETVRMSAPRTGGGFAVWGGLFSTFDCTMVYIRQKEDPWNSIFAGAATGGFLQVRQGLRASGRSAVFGGILLALIEGAGIMLNRVVSPPAQIMYEDPSMGAGAPQMGGYGFGFPGQARAPANVGGGGSEEVNQGGSGGGGFFGGWFGGDGKKEGSSSSSGGSEATVLESFDTPTPMPNFDYK

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo02971	Spo02971	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo02971.1	Spo02971.1-protein	polypeptide

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo02971.1.exon.1	Spo02971.1.exon.1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo02971.1.CDS.1	Spo02971.1.CDS.1	CDS

Homology

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BLAST of Spo02971.1 vs. NCBI nr
Match: gi|731355407|ref|XP_010689124.1| (PREDICTED: mitochondrial import inner membrane translocase subunit TIM17-2 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 367.5 bits (942), Expect = 1.800e-98
Identity = 192/245 (78.37%), Postives = 212/245 (86.53%), Query Frame = 1


		  
Query: 1   MGTPETSREPCPDRILDDLGGAFAMGAIGGSVFHYVRGSINAPKGERILRALETVRMSAP 60
           MGTPETSREPCPDRILDDLGGAFAMGA+GGS+FH+++GS N+PKGER+L   +T+RM+AP
Sbjct: 1   MGTPETSREPCPDRILDDLGGAFAMGAVGGSIFHFIKGSANSPKGERLLGGAQTMRMNAP 60

Query: 61  RTGGGFAVWGGLFSTFDCTMVYIRQKEDPWNSIFAGAATGGFLQVRQGLRASGRSAVFGG 120
           RTGGGFAVWGGLFSTFDC MVY RQKEDPWNSIFAGAATGGFLQ+RQGL+AS RSA FGG
Sbjct: 61  RTGGGFAVWGGLFSTFDCAMVYARQKEDPWNSIFAGAATGGFLQMRQGLKASSRSAAFGG 120

Query: 121 ILLALIEGAGIMLNRVVSPPAQIMYEDPSM------GAGAPQMGGYGFGFPGQARAPANV 180
           ILLALIEGAGIMLNRV+SPP QIMYEDP+M      G  AP MG  GFG PGQ +AP+N+
Sbjct: 121 ILLALIEGAGIMLNRVMSPPPQIMYEDPNMAGVGMGGQHAP-MGNAGFGIPGQMKAPSNI 180

Query: 181 GGGGSEEVNQGGSGGG---GFFGGWFGG--DGKKEGSSSSSG-GSEATVLESFDTPTPMP 234
           GGG SEEVN GGS GG   GFFGGWFGG  DGKKEG +SSSG GSEA VLESFD+PTPMP
Sbjct: 181 GGG-SEEVNHGGSAGGAGSGFFGGWFGGGGDGKKEGKTSSSGGGSEAKVLESFDSPTPMP 240

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BLAST of Spo02971.1 vs. NCBI nr
Match: gi|902184119|gb|KNA10244.1| (hypothetical protein SOVF_146150 [Spinacia oleracea])

HSP 1 Score: 358.2 bits (918), Expect = 1.100e-95
Identity = 176/176 (100.00%), Postives = 176/176 (100.00%), Query Frame = 1


		  
Query: 1   MGTPETSREPCPDRILDDLGGAFAMGAIGGSVFHYVRGSINAPKGERILRALETVRMSAP 60
           MGTPETSREPCPDRILDDLGGAFAMGAIGGSVFHYVRGSINAPKGERILRALETVRMSAP
Sbjct: 1   MGTPETSREPCPDRILDDLGGAFAMGAIGGSVFHYVRGSINAPKGERILRALETVRMSAP 60

Query: 61  RTGGGFAVWGGLFSTFDCTMVYIRQKEDPWNSIFAGAATGGFLQVRQGLRASGRSAVFGG 120
           RTGGGFAVWGGLFSTFDCTMVYIRQKEDPWNSIFAGAATGGFLQVRQGLRASGRSAVFGG
Sbjct: 61  RTGGGFAVWGGLFSTFDCTMVYIRQKEDPWNSIFAGAATGGFLQVRQGLRASGRSAVFGG 120

Query: 121 ILLALIEGAGIMLNRVVSPPAQIMYEDPSMGAGAPQMGGYGFGFPGQARAPANVGG 177
           ILLALIEGAGIMLNRVVSPPAQIMYEDPSMGAGAPQMGGYGFGFPGQARAPANVGG
Sbjct: 121 ILLALIEGAGIMLNRVVSPPAQIMYEDPSMGAGAPQMGGYGFGFPGQARAPANVGG 176

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BLAST of Spo02971.1 vs. NCBI nr
Match: gi|902185286|gb|KNA10470.1| (hypothetical protein SOVF_144160 [Spinacia oleracea])

HSP 1 Score: 337.8 bits (865), Expect = 1.500e-89
Identity = 169/177 (95.48%), Postives = 172/177 (97.18%), Query Frame = 1


		  
Query: 1   MGTPETSREPCPDRILDDLGGAFAMGAIGGSVFHYVRGSINAPKGERILRALETVRMSAP 60
           MGTPETSREPCPDRILDDLGGAFAMGA+GGSVFHYVRGSINAPKGERILRALETVRMSAP
Sbjct: 1   MGTPETSREPCPDRILDDLGGAFAMGAVGGSVFHYVRGSINAPKGERILRALETVRMSAP 60

Query: 61  RTGGGFAVWGGLFSTFDCTMVYIRQKEDPWNSIFAGAATGGFLQVRQGLRASGRSAVFGG 120
           RTGGGFAVWGGLFSTFDCT+VYIRQKEDPWNSIFAGAATGGFLQVRQGLRASGRSA FGG
Sbjct: 61  RTGGGFAVWGGLFSTFDCTLVYIRQKEDPWNSIFAGAATGGFLQVRQGLRASGRSAAFGG 120

Query: 121 ILLALIEGAGIMLNRVVSPPAQIMYEDPSMGAGAP-QMGGYGFGFPGQARAPANVGG 177
           ILLALIEGAGIMLNRV+SPPAQIMYEDPSMGAGAP QMGGYGFGFPGQA   ANVGG
Sbjct: 121 ILLALIEGAGIMLNRVMSPPAQIMYEDPSMGAGAPQQMGGYGFGFPGQA---ANVGG 174

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BLAST of Spo02971.1 vs. NCBI nr
Match: gi|802627893|ref|XP_012076870.1| (PREDICTED: mitochondrial import inner membrane translocase subunit TIM17-2-like [Jatropha curcas])

HSP 1 Score: 281.6 bits (719), Expect = 1.300e-72
Identity = 153/238 (64.29%), Postives = 181/238 (76.05%), Query Frame = 1


		  
Query: 1   MGTPETSREPCPDRILDDLGGAFAMGAIGGSVFHYVRGSINAPKGERILRALETVRMSAP 60
           MGTPETSREPCPDRILDD+GGAF MGA+GGS FH+++G  N+P G R++   + VRM+AP
Sbjct: 1   MGTPETSREPCPDRILDDIGGAFGMGAVGGSAFHFLKGIYNSPSGARLIGGTQAVRMNAP 60

Query: 61  RTGGGFAVWGGLFSTFDCTMVYIRQKEDPWNSIFAGAATGGFLQVRQGLRASGRSAVFGG 120
           R GG FAVWGGLFS FDCTMVY+RQKEDPWNSIFAGAATGGFL +RQGL AS RSAVFGG
Sbjct: 61  RVGGSFAVWGGLFSAFDCTMVYVRQKEDPWNSIFAGAATGGFLSMRQGLGASARSAVFGG 120

Query: 121 ILLALIEGAGIMLNRVVSPPAQ--IMYED--PSMGAGAPQMGGYGFGFPGQARA-PANVG 180
           ILLALIEGAGIMLN+V+S      I+ ED  P+M AG P   G+  G PGQ +  P  V 
Sbjct: 121 ILLALIEGAGIMLNKVMSAQQNMPIIIEDSVPAM-AGGP---GFPMGIPGQPQPHPQTV- 180

Query: 181 GGGSEEVNQGGSGGGGFFGGWFGGDGKKEGSSSSSGGSEATVLESFDTPTPMPNFDYK 234
               +E +  GS  G +FGGWFG  GKKE +++SS GS+  +LESFD P P+PNF+YK
Sbjct: 181 ----QEASTSGSESGSWFGGWFG--GKKEPAAASSSGSKTEILESFDAP-PVPNFEYK 226

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BLAST of Spo02971.1 vs. NCBI nr
Match: gi|720035749|ref|XP_010267135.1| (PREDICTED: mitochondrial import inner membrane translocase subunit TIM17-2-like [Nelumbo nucifera])

HSP 1 Score: 277.3 bits (708), Expect = 2.400e-71
Identity = 151/236 (63.98%), Postives = 178/236 (75.42%), Query Frame = 1


		  
Query: 1   MGTPETSREPCPDRILDDLGGAFAMGAIGGSVFHYVRGSINAPKGERILRALETVRMSAP 60
           MGTPETSREPCPDRILDD+GGAF MGA+GGS FH+++G  N+PKGER++   + VRM+AP
Sbjct: 1   MGTPETSREPCPDRILDDIGGAFGMGAVGGSAFHFLKGIYNSPKGERLIGGSQAVRMNAP 60

Query: 61  RTGGGFAVWGGLFSTFDCTMVYIRQKEDPWNSIFAGAATGGFLQVRQGLRASGRSAVFGG 120
           R GG FAVWGGLFS FDC+MVYIRQKEDPWNSIFAGAATGGFLQ+RQGL A+ RSA+FGG
Sbjct: 61  RVGGSFAVWGGLFSVFDCSMVYIRQKEDPWNSIFAGAATGGFLQMRQGLGAASRSALFGG 120

Query: 121 ILLALIEGAGIMLNRVVSPPAQIMYEDPSMGAGAPQMGGYGFGFP-GQ-ARAPANVGGGG 180
           +LLALIEGAGIMLN+V+S P       P M    P M G G GFP GQ   APA +    
Sbjct: 121 VLLALIEGAGIMLNKVLSVPQNF---PPMMEEPLPNMAG-GPGFPMGQLPSAPATI---- 180

Query: 181 SEEVNQGGSGGGGFFGGWFGGDGKKEGSSSSSGGSEATVLESFDTP-TPMPNFDYK 234
            E   +G S    + GG FGG  K+E  +SS GGS+  +LESFDTP TP+P+F+YK
Sbjct: 181 -EPSTEGSS--SSWIGGLFGGGNKQEKGASSGGGSKTEILESFDTPSTPIPSFEYK 225

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BLAST of Spo02971.1 vs. UniProtKB/TrEMBL
Match: A0A0J8BLQ9_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_9g205600 PE=4 SV=1)

HSP 1 Score: 367.5 bits (942), Expect = 1.200e-98
Identity = 192/245 (78.37%), Postives = 212/245 (86.53%), Query Frame = 1


		  
Query: 1   MGTPETSREPCPDRILDDLGGAFAMGAIGGSVFHYVRGSINAPKGERILRALETVRMSAP 60
           MGTPETSREPCPDRILDDLGGAFAMGA+GGS+FH+++GS N+PKGER+L   +T+RM+AP
Sbjct: 1   MGTPETSREPCPDRILDDLGGAFAMGAVGGSIFHFIKGSANSPKGERLLGGAQTMRMNAP 60

Query: 61  RTGGGFAVWGGLFSTFDCTMVYIRQKEDPWNSIFAGAATGGFLQVRQGLRASGRSAVFGG 120
           RTGGGFAVWGGLFSTFDC MVY RQKEDPWNSIFAGAATGGFLQ+RQGL+AS RSA FGG
Sbjct: 61  RTGGGFAVWGGLFSTFDCAMVYARQKEDPWNSIFAGAATGGFLQMRQGLKASSRSAAFGG 120

Query: 121 ILLALIEGAGIMLNRVVSPPAQIMYEDPSM------GAGAPQMGGYGFGFPGQARAPANV 180
           ILLALIEGAGIMLNRV+SPP QIMYEDP+M      G  AP MG  GFG PGQ +AP+N+
Sbjct: 121 ILLALIEGAGIMLNRVMSPPPQIMYEDPNMAGVGMGGQHAP-MGNAGFGIPGQMKAPSNI 180

Query: 181 GGGGSEEVNQGGSGGG---GFFGGWFGG--DGKKEGSSSSSG-GSEATVLESFDTPTPMP 234
           GGG SEEVN GGS GG   GFFGGWFGG  DGKKEG +SSSG GSEA VLESFD+PTPMP
Sbjct: 181 GGG-SEEVNHGGSAGGAGSGFFGGWFGGGGDGKKEGKTSSSGGGSEAKVLESFDSPTPMP 240

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BLAST of Spo02971.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QSK3_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_146150 PE=4 SV=1)

HSP 1 Score: 358.2 bits (918), Expect = 7.500e-96
Identity = 176/176 (100.00%), Postives = 176/176 (100.00%), Query Frame = 1


		  
Query: 1   MGTPETSREPCPDRILDDLGGAFAMGAIGGSVFHYVRGSINAPKGERILRALETVRMSAP 60
           MGTPETSREPCPDRILDDLGGAFAMGAIGGSVFHYVRGSINAPKGERILRALETVRMSAP
Sbjct: 1   MGTPETSREPCPDRILDDLGGAFAMGAIGGSVFHYVRGSINAPKGERILRALETVRMSAP 60

Query: 61  RTGGGFAVWGGLFSTFDCTMVYIRQKEDPWNSIFAGAATGGFLQVRQGLRASGRSAVFGG 120
           RTGGGFAVWGGLFSTFDCTMVYIRQKEDPWNSIFAGAATGGFLQVRQGLRASGRSAVFGG
Sbjct: 61  RTGGGFAVWGGLFSTFDCTMVYIRQKEDPWNSIFAGAATGGFLQVRQGLRASGRSAVFGG 120

Query: 121 ILLALIEGAGIMLNRVVSPPAQIMYEDPSMGAGAPQMGGYGFGFPGQARAPANVGG 177
           ILLALIEGAGIMLNRVVSPPAQIMYEDPSMGAGAPQMGGYGFGFPGQARAPANVGG
Sbjct: 121 ILLALIEGAGIMLNRVVSPPAQIMYEDPSMGAGAPQMGGYGFGFPGQARAPANVGG 176

[Close]

BLAST of Spo02971.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QT95_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_144160 PE=4 SV=1)

HSP 1 Score: 337.8 bits (865), Expect = 1.000e-89
Identity = 169/177 (95.48%), Postives = 172/177 (97.18%), Query Frame = 1


		  
Query: 1   MGTPETSREPCPDRILDDLGGAFAMGAIGGSVFHYVRGSINAPKGERILRALETVRMSAP 60
           MGTPETSREPCPDRILDDLGGAFAMGA+GGSVFHYVRGSINAPKGERILRALETVRMSAP
Sbjct: 1   MGTPETSREPCPDRILDDLGGAFAMGAVGGSVFHYVRGSINAPKGERILRALETVRMSAP 60

Query: 61  RTGGGFAVWGGLFSTFDCTMVYIRQKEDPWNSIFAGAATGGFLQVRQGLRASGRSAVFGG 120
           RTGGGFAVWGGLFSTFDCT+VYIRQKEDPWNSIFAGAATGGFLQVRQGLRASGRSA FGG
Sbjct: 61  RTGGGFAVWGGLFSTFDCTLVYIRQKEDPWNSIFAGAATGGFLQVRQGLRASGRSAAFGG 120

Query: 121 ILLALIEGAGIMLNRVVSPPAQIMYEDPSMGAGAP-QMGGYGFGFPGQARAPANVGG 177
           ILLALIEGAGIMLNRV+SPPAQIMYEDPSMGAGAP QMGGYGFGFPGQA   ANVGG
Sbjct: 121 ILLALIEGAGIMLNRVMSPPAQIMYEDPSMGAGAPQQMGGYGFGFPGQA---ANVGG 174

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BLAST of Spo02971.1 vs. UniProtKB/TrEMBL
Match: A0A067KNE2_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_07366 PE=4 SV=1)

HSP 1 Score: 281.6 bits (719), Expect = 8.900e-73
Identity = 153/238 (64.29%), Postives = 181/238 (76.05%), Query Frame = 1


		  
Query: 1   MGTPETSREPCPDRILDDLGGAFAMGAIGGSVFHYVRGSINAPKGERILRALETVRMSAP 60
           MGTPETSREPCPDRILDD+GGAF MGA+GGS FH+++G  N+P G R++   + VRM+AP
Sbjct: 1   MGTPETSREPCPDRILDDIGGAFGMGAVGGSAFHFLKGIYNSPSGARLIGGTQAVRMNAP 60

Query: 61  RTGGGFAVWGGLFSTFDCTMVYIRQKEDPWNSIFAGAATGGFLQVRQGLRASGRSAVFGG 120
           R GG FAVWGGLFS FDCTMVY+RQKEDPWNSIFAGAATGGFL +RQGL AS RSAVFGG
Sbjct: 61  RVGGSFAVWGGLFSAFDCTMVYVRQKEDPWNSIFAGAATGGFLSMRQGLGASARSAVFGG 120

Query: 121 ILLALIEGAGIMLNRVVSPPAQ--IMYED--PSMGAGAPQMGGYGFGFPGQARA-PANVG 180
           ILLALIEGAGIMLN+V+S      I+ ED  P+M AG P   G+  G PGQ +  P  V 
Sbjct: 121 ILLALIEGAGIMLNKVMSAQQNMPIIIEDSVPAM-AGGP---GFPMGIPGQPQPHPQTV- 180

Query: 181 GGGSEEVNQGGSGGGGFFGGWFGGDGKKEGSSSSSGGSEATVLESFDTPTPMPNFDYK 234
               +E +  GS  G +FGGWFG  GKKE +++SS GS+  +LESFD P P+PNF+YK
Sbjct: 181 ----QEASTSGSESGSWFGGWFG--GKKEPAAASSSGSKTEILESFDAP-PVPNFEYK 226

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BLAST of Spo02971.1 vs. UniProtKB/TrEMBL
Match: W9SV38_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_006999 PE=4 SV=1)

HSP 1 Score: 276.9 bits (707), Expect = 2.200e-71
Identity = 150/239 (62.76%), Postives = 174/239 (72.80%), Query Frame = 1


		  
Query: 1   MGTPETSREPCPDRILDDLGGAFAMGAIGGSVFHYVRGSINAPKGERILRALETVRMSAP 60
           MGTPETSREPCPDRILDD+GGAF MGA+GGS FH+++G  N+P+G R++   + VRM+AP
Sbjct: 1   MGTPETSREPCPDRILDDIGGAFGMGAVGGSAFHFLKGIYNSPRGSRLVGGSQAVRMNAP 60

Query: 61  RTGGGFAVWGGLFSTFDCTMVYIRQKEDPWNSIFAGAATGGFLQVRQGLRASGRSAVFGG 120
           R GG FAVWGGLFS FDCTMVY+RQKEDPWNSI AGAATGGFLQ+RQG  ASGRSA+FGG
Sbjct: 61  RVGGSFAVWGGLFSAFDCTMVYVRQKEDPWNSIIAGAATGGFLQMRQGPAASGRSALFGG 120

Query: 121 ILLALIEGAGIMLNRVVSPPAQ--IMYEDPSMGA----GAPQMGGYGFGFPGQARAPANV 180
           +LLALIEGAGIMLN+V+S   Q  IM E+P        G P MG      PGQ      V
Sbjct: 121 VLLALIEGAGIMLNKVLSQQQQMPIMIEEPPPNVASIPGFPPMGQIPGRLPGQQVPEVAV 180

Query: 181 GGGGSEEVNQGGSGGGGFFGGWFGGDGKKEGSSSSSGGSEATVLESFDTPTPMPNFDYK 234
           G   S +    GS    F GGWFGG  KKE  +SSS GSE  +LESFD P P+P+F+YK
Sbjct: 181 GSASSSDSADSGS----FVGGWFGGGKKKEPQASSS-GSETKILESFDAP-PVPSFEYK 233

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BLAST of Spo02971.1 vs. ExPASy Swiss-Prot
Match: TI172_ARATH (Mitochondrial import inner membrane translocase subunit TIM17-2 OS=Arabidopsis thaliana GN=TIM17-2 PE=1 SV=2)

HSP 1 Score: 253.8 bits (647), Expect = 1.800e-66
Identity = 141/248 (56.85%), Postives = 171/248 (68.95%), Query Frame = 1


		  
Query: 1   MGTPETSREPCPDRILDDLGGAFAMGAIGGSVFHYVRGSINAPKGERILRALETVRMSAP 60
           MGTPETSREPCPDRILDD+GGAF MGA+GGS FH+++G+ N+PKG R +   ++V M+AP
Sbjct: 1   MGTPETSREPCPDRILDDIGGAFGMGAVGGSAFHFIKGTYNSPKGSRFVGGTQSVSMNAP 60

Query: 61  RTGGGFAVWGGLFSTFDCTMVYIRQKEDPWNSIFAGAATGGFLQVRQGLRASGRSAVFGG 120
           RTGG FAVWGGLFSTFDCTMVY+RQKEDPWNSI AGAATGGFL +RQG  A+ RSA+FGG
Sbjct: 61  RTGGSFAVWGGLFSTFDCTMVYLRQKEDPWNSIIAGAATGGFLSMRQGAGAASRSAIFGG 120

Query: 121 ILLALIEGAGIMLNRVVSPPAQIMYEDPSMGA--------GAPQMGGYGFGFP------- 180
           +LLALIEGAGIMLN+V++ P  +M EDP M          G P M G   G P       
Sbjct: 121 VLLALIEGAGIMLNKVLAQPQNMMMEDPGMQGMPGMQGMQGMPGMPGMQ-GMPGMQGMQM 180

Query: 181 GQARAPANVGGGGSEEVNQGGSGGGGFFGGWFGGDGKKEGSSSSSGGSEATVLESFDTPT 234
           GQ ++ A +      +     S    +FGG F  D KKE     S  S+  VLESFD P 
Sbjct: 181 GQMQSQAQIRSESQNQNTASSSSSSSWFGGLF--DKKKEEVQPGS-ESKTEVLESFDAP- 240

[Close]

BLAST of Spo02971.1 vs. ExPASy Swiss-Prot
Match: TI171_ARATH (Mitochondrial import inner membrane translocase subunit TIM17-1 OS=Arabidopsis thaliana GN=TIM17-1 PE=2 SV=1)

HSP 1 Score: 223.4 bits (568), Expect = 2.600e-57
Identity = 120/233 (51.50%), Postives = 151/233 (64.81%), Query Frame = 1


		  
Query: 1   MGTPETSREPCPDRILDDLGGAFAMGAIGGSVFHYVRGSINAPKGERILRALETVRMSAP 60
           MGTPE+SREPCPDRILDD+GGAFAMGA+GGS +H +RG  N+P G R+   ++ +RMS P
Sbjct: 1   MGTPESSREPCPDRILDDVGGAFAMGAVGGSAYHLIRGIYNSPGGARLSGGVQALRMSGP 60

Query: 61  RTGGGFAVWGGLFSTFDCTMVYIRQKEDPWNSIFAGAATGGFLQVRQGLRASGRSAVFGG 120
           R+GG F+VWGGL+STFDC +VY RQKEDPWNSI +GAATGGFL +RQGL AS RSA+ GG
Sbjct: 61  RSGGSFSVWGGLYSTFDCALVYARQKEDPWNSILSGAATGGFLSLRQGLGASARSALVGG 120

Query: 121 ILLALIEGAGIMLNRVVSPPAQIMYEDPSMGAGAPQMGGYGFGFPGQARAPANVGGGGSE 180
           +LLA+IEG GIMLN+V S      + +       P    YG G       P         
Sbjct: 121 VLLAMIEGVGIMLNKVQSTAHNEQFMEDHAATSLP----YGMGQISGQSVPV-------P 180

Query: 181 EVNQGGSGGGGFFGGWFGGDGKKEGSSSSSGGSEATVLESFDTPTPMPNFDYK 234
           E +   SG   +FG  F    KK+ +      S   +LESFD P P+P +++K
Sbjct: 181 ETSSSSSGSVSWFGSLF---KKKKETEDHHSESRTHILESFDAP-PVPTYEFK 218

[Close]

BLAST of Spo02971.1 vs. ExPASy Swiss-Prot
Match: TI17A_HUMAN (Mitochondrial import inner membrane translocase subunit Tim17-A OS=Homo sapiens GN=TIMM17A PE=1 SV=1)

HSP 1 Score: 160.2 bits (404), Expect = 2.700e-38
Identity = 83/150 (55.33%), Postives = 98/150 (65.33%), Query Frame = 1


		  
Query: 5   ETSREPCPDRILDDLGGAFAMGAIGGSVFHYVRGSINAPKG--ERILRALETVRMSAPRT 64
           E +REPCP RI+DD GGAF MG IGG +F  ++G  N+P G   R+  +L  ++  AP+ 
Sbjct: 3   EYAREPCPWRIVDDCGGAFTMGTIGGGIFQAIKGFRNSPVGVNHRLRGSLTAIKTRAPQL 62

Query: 65  GGGFAVWGGLFSTFDCTMVYIRQKEDPWNSIFAGAATGGFLQVRQGLRASGRSAVFGGIL 124
           GG FAVWGGLFS  DC+MV +R KEDPWNSI +GA TG  L  R G  A   SA  GGIL
Sbjct: 63  GGSFAVWGGLFSMIDCSMVQVRGKEDPWNSITSGALTGAILAARNGPVAMVGSAAMGGIL 122

Query: 125 LALIEGAGIMLNRVVS---PPAQIMYEDPS 150
           LALIEGAGI+L R  S   P      EDPS
Sbjct: 123 LALIEGAGILLTRFASAQFPNGPQFAEDPS 152

[Close]

BLAST of Spo02971.1 vs. ExPASy Swiss-Prot
Match: TI17A_MOUSE (Mitochondrial import inner membrane translocase subunit Tim17-A OS=Mus musculus GN=Timm17a PE=1 SV=1)

HSP 1 Score: 158.3 bits (399), Expect = 1.000e-37
Identity = 80/136 (58.82%), Postives = 93/136 (68.38%), Query Frame = 1


		  
Query: 5   ETSREPCPDRILDDLGGAFAMGAIGGSVFHYVRGSINAPKG--ERILRALETVRMSAPRT 64
           E +REPCP RI+DD GGAF MG IGG +F   +G  N+P G   R+  +L  ++  AP+ 
Sbjct: 3   EYAREPCPWRIVDDCGGAFTMGTIGGGIFQAFKGFRNSPVGINHRLRGSLTAIKTRAPQL 62

Query: 65  GGGFAVWGGLFSTFDCTMVYIRQKEDPWNSIFAGAATGGFLQVRQGLRASGRSAVFGGIL 124
           GG FAVWGGLFST DC+MV IR KEDPWNSI +GA TG  L  R G  A   SA  GGIL
Sbjct: 63  GGSFAVWGGLFSTIDCSMVQIRGKEDPWNSITSGALTGAILAARNGPVAMVGSAAMGGIL 122

Query: 125 LALIEGAGIMLNRVVS 139
           LALIEGAGI+L R  S
Sbjct: 123 LALIEGAGILLTRFAS 138

[Close]

BLAST of Spo02971.1 vs. ExPASy Swiss-Prot
Match: TI17B_MOUSE (Mitochondrial import inner membrane translocase subunit Tim17-B OS=Mus musculus GN=Timm17b PE=1 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 1.700e-37
Identity = 88/166 (53.01%), Postives = 104/166 (62.65%), Query Frame = 1


		  
Query: 5   ETSREPCPDRILDDLGGAFAMGAIGGSVFHYVRGSINAPKG--ERILRALETVRMSAPRT 64
           E +REPCP RI+DD GGAF MG IGG VF  ++G  NAP G   R   ++  VR+ AP+ 
Sbjct: 3   EYAREPCPWRIVDDCGGAFTMGVIGGGVFQAIKGFRNAPVGIRHRFRGSVNAVRIRAPQI 62

Query: 65  GGGFAVWGGLFSTFDCTMVYIRQKEDPWNSIFAGAATGGFLQVRQGLRASGRSAVFGGIL 124
           GG FAVWGGLFST DC +V +R KEDPWNSI +GA TG  L  R G  A   SA+ GGIL
Sbjct: 63  GGSFAVWGGLFSTIDCGLVRLRGKEDPWNSISSGALTGAVLAARSGPLAMVGSAMMGGIL 122

Query: 125 LALIEGAGIMLNRVVSPP---AQIMYEDPSMGAGAPQMGGYGFGFP 166
           LALIEG GI+L R  +     A    EDPS     P+ G    G+P
Sbjct: 123 LALIEGVGILLTRYTAQQFRNAPPFLEDPSQ--LTPKEGSPAPGYP 166

[Close]

BLAST of Spo02971.1 vs. TAIR (Arabidopsis)
Match: AT2G37410.1 (translocase inner membrane subunit 17-2)

HSP 1 Score: 253.8 bits (647), Expect = 1.000e-67
Identity = 141/248 (56.85%), Postives = 171/248 (68.95%), Query Frame = 1


		  
Query: 1   MGTPETSREPCPDRILDDLGGAFAMGAIGGSVFHYVRGSINAPKGERILRALETVRMSAP 60
           MGTPETSREPCPDRILDD+GGAF MGA+GGS FH+++G+ N+PKG R +   ++V M+AP
Sbjct: 1   MGTPETSREPCPDRILDDIGGAFGMGAVGGSAFHFIKGTYNSPKGSRFVGGTQSVSMNAP 60

Query: 61  RTGGGFAVWGGLFSTFDCTMVYIRQKEDPWNSIFAGAATGGFLQVRQGLRASGRSAVFGG 120
           RTGG FAVWGGLFSTFDCTMVY+RQKEDPWNSI AGAATGGFL +RQG  A+ RSA+FGG
Sbjct: 61  RTGGSFAVWGGLFSTFDCTMVYLRQKEDPWNSIIAGAATGGFLSMRQGAGAASRSAIFGG 120

Query: 121 ILLALIEGAGIMLNRVVSPPAQIMYEDPSMGA--------GAPQMGGYGFGFP------- 180
           +LLALIEGAGIMLN+V++ P  +M EDP M          G P M G   G P       
Sbjct: 121 VLLALIEGAGIMLNKVLAQPQNMMMEDPGMQGMPGMQGMQGMPGMPGMQ-GMPGMQGMQM 180

Query: 181 GQARAPANVGGGGSEEVNQGGSGGGGFFGGWFGGDGKKEGSSSSSGGSEATVLESFDTPT 234
           GQ ++ A +      +     S    +FGG F  D KKE     S  S+  VLESFD P 
Sbjct: 181 GQMQSQAQIRSESQNQNTASSSSSSSWFGGLF--DKKKEEVQPGS-ESKTEVLESFDAP- 240

[Close]

BLAST of Spo02971.1 vs. TAIR (Arabidopsis)
Match: AT1G20350.1 (translocase inner membrane subunit 17-1)

HSP 1 Score: 223.4 bits (568), Expect = 1.500e-58
Identity = 120/233 (51.50%), Postives = 151/233 (64.81%), Query Frame = 1


		  
Query: 1   MGTPETSREPCPDRILDDLGGAFAMGAIGGSVFHYVRGSINAPKGERILRALETVRMSAP 60
           MGTPE+SREPCPDRILDD+GGAFAMGA+GGS +H +RG  N+P G R+   ++ +RMS P
Sbjct: 1   MGTPESSREPCPDRILDDVGGAFAMGAVGGSAYHLIRGIYNSPGGARLSGGVQALRMSGP 60

Query: 61  RTGGGFAVWGGLFSTFDCTMVYIRQKEDPWNSIFAGAATGGFLQVRQGLRASGRSAVFGG 120
           R+GG F+VWGGL+STFDC +VY RQKEDPWNSI +GAATGGFL +RQGL AS RSA+ GG
Sbjct: 61  RSGGSFSVWGGLYSTFDCALVYARQKEDPWNSILSGAATGGFLSLRQGLGASARSALVGG 120

Query: 121 ILLALIEGAGIMLNRVVSPPAQIMYEDPSMGAGAPQMGGYGFGFPGQARAPANVGGGGSE 180
           +LLA+IEG GIMLN+V S      + +       P    YG G       P         
Sbjct: 121 VLLAMIEGVGIMLNKVQSTAHNEQFMEDHAATSLP----YGMGQISGQSVPV-------P 180

Query: 181 EVNQGGSGGGGFFGGWFGGDGKKEGSSSSSGGSEATVLESFDTPTPMPNFDYK 234
           E +   SG   +FG  F    KK+ +      S   +LESFD P P+P +++K
Sbjct: 181 ETSSSSSGSVSWFGSLF---KKKKETEDHHSESRTHILESFDAP-PVPTYEFK 218

[Close]

BLAST of Spo02971.1 vs. TAIR (Arabidopsis)
Match: AT5G11690.1 (translocase inner membrane subunit 17-3)

HSP 1 Score: 136.0 bits (341), Expect = 3.100e-32
Identity = 67/126 (53.17%), Postives = 89/126 (70.63%), Query Frame = 1


		  
Query: 1   MGTPETSREPCPDRILDDLGGAFAMGAIGGSVFHYVRGSINAPKGERILRALETVRMSAP 60
           M T + S+E    RI++ +G AF  GA+GGSV+H+VRG+ N+P G R +   +   M+AP
Sbjct: 1   MDTKKKSKEHGLYRIVNAIGYAFGAGAVGGSVYHFVRGAYNSPIGARYVGGTQAASMNAP 60

Query: 61  RTGGGFAVWGGLFSTFDCTMVYIRQKEDPWNSIFAGAATGGFLQVRQGLRASGRSAVFGG 120
           R GG FAV+GGL STFD  +V IR+KEDPWNSI AGAATGG L +R+G+ A+  SAV  G
Sbjct: 61  RLGGTFAVFGGLLSTFDYALVRIRKKEDPWNSIVAGAATGGVLSIRKGVVAASTSAVMFG 120

Query: 121 ILLALI 127
             LA++
Sbjct: 121 FFLAVL 126

The following BLAST results are available for this feature:

BLAST of Spo02971.1 vs. NCBI nr
BLAST of Spo02971.1 vs. UniProtKB/TrEMBL
BLAST of Spo02971.1 vs. ExPASy Swiss-Prot
BLAST of Spo02971.1 vs. TAIR (Arabidopsis)

BLAST of Spo02971.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|731355407\|ref\|XP_010689124.1\|	1.8e-98	78.3	PREDICTED: mitochondrial impor...	[more]
gi\|902184119\|gb\|KNA10244.1\|	1.1e-95	100.	hypothetical protein SOVF_1461...	[more]
gi\|902185286\|gb\|KNA10470.1\|	1.5e-89	95.4	hypothetical protein SOVF_1441...	[more]
gi\|802627893\|ref\|XP_012076870.1\|	1.3e-72	64.2	PREDICTED: mitochondrial impor...	[more]
gi\|720035749\|ref\|XP_010267135.1\|	2.4e-71	63.9	PREDICTED: mitochondrial impor...	[more]

BLAST of Spo02971.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0J8BLQ9_BETVU	1.2e-98	78.3	Uncharacterized protein OS=Bet...	[more]
A0A0K9QSK3_SPIOL	7.5e-96	100.	Uncharacterized protein OS=Spi...	[more]
A0A0K9QT95_SPIOL	1.0e-89	95.4	Uncharacterized protein OS=Spi...	[more]
A0A067KNE2_JATCU	8.9e-73	64.2	Uncharacterized protein OS=Jat...	[more]
W9SV38_9ROSA	2.2e-71	62.7	Uncharacterized protein OS=Mor...	[more]

BLAST of Spo02971.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
TI172_ARATH	1.8e-66	56.8	Mitochondrial import inner mem...	[more]
TI171_ARATH	2.6e-57	51.5	Mitochondrial import inner mem...	[more]
TI17A_HUMAN	2.7e-38	55.3	Mitochondrial import inner mem...	[more]
TI17A_MOUSE	1.0e-37	58.8	Mitochondrial import inner mem...	[more]
TI17B_MOUSE	1.7e-37	53.0	Mitochondrial import inner mem...	[more]

BLAST of Spo02971.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 3


Match Name	E-value	Identity	Description
AT2G37410.1	1.0e-67	56.8	translocase inner membrane sub...	[more]
AT1G20350.1	1.5e-58	51.5	translocase inner membrane sub...	[more]
AT5G11690.1	3.1e-32	53.1	translocase inner membrane sub...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003397	Mitochondrial inner membrane translocase subunit Tim17/Tim22/Tim23/peroxisomal protein PMP24	PFAM	PF02466	Tim17	coord: 16..122 score: 2.6
IPR005678	Mitochondrial inner membrane translocase complex, subunit Tim17	PANTHER	PTHR10485	MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM-17	coord: 1..233 score: 2.5E
None	No IPR available	PANTHER	PTHR10485:SF0	AT05822P-RELATED	coord: 1..233 score: 2.5E

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006886	intracellular protein transport
biological_process	GO:0009987	cellular process
cellular_component	GO:0005744	mitochondrial inner membrane presequence translocase complex
cellular_component	GO:0009941	chloroplast envelope
cellular_component	GO:0044425	membrane part
molecular_function	GO:0015450	P-P-bond-hydrolysis-driven protein transmembrane transporter activity