Spo03048.1 (mRNA) | SpinachBase

Overview


Name	Spo03048.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	Ubiquitin-protein ligase 1
Location	SpoScf_10199 : 156 .. 2428 (+)
Sequence length	1539

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TGGAAGTCTTTCAGCCACCAATCCCAGAGGCTCTATGGTTGCCCCTCAAATGTGGTCTATGGTCATTCCCAGTTGTTAGAGGGTATGCACTTCCCGAGCATAGCCTGTGTGCTCTACATTTTTAGTGGTCTTCTAACAGAACCTTTTTGCAGGTCCTCCACCCTTGGTTTTGCGTCTTGTTCTTGAGATTTTAAATTATTTGGCCACCAATCATTCTGCTGTTGCAAACACGTTGTTCTACTTTGAGCCATCACTTTTGCCCGAGTCTTTCCAGAAGTATGCAGTAAACAAAAAGGGAAAGGGCAAAGAAAAAGTAGGGGAAGCTGGAGTGCCTCCAAACTCTGTTCAGGAATACAAGGAAGGGGGTACTCCTTTAATATTGTTGTTGAAGCTTTTGAATCGCCCTCTTTTCCGTCGTAGCATTGCTCATGTTGAGCAGGTAGGTCTGATGATTTTTATTACAAGTATCCTTTTGGTAATGTATACTGAATACCGATAATCTTGTATCTTAATTGTTAAACAGGTTATGGGTCTGCTTCAAGTGGTGGTCTATACTGCAGCGTCAAGATTAGAGAGCCCGTCTCTCCCTGAGCAGGTCTTAGATGATTCTCAGAATCTTCCTAGTAAAGCTTCTGATGAGGTAGAAAGAGATGTTGTTTTGGTGGAAACAGAGACTAATAAACAGAATAAAGTTGAAAATGGGGAACCCTCTATATCTGATAGGAGTACAGGTCATAATATCCATGATGTCTTCACGCAGCTGCCAAAATCTGATTTGCGTTATCTCTCCCGTCTTCTTGGCTATGAGGGGTATGAATTGTGTGCATTTTTATTCAGACGTGGTTTTATGTTATTTCTTCCTCGTTATTATCAATATAAATGTTAGGTGTAATTCCTATTACCATGATCAATAGAGCCATTCTGATTCAACCTCTGCTGACTCACTCATTAACCTAGAAATATGAGGAATCAGTTCCGTCTTTTTTTGTTAGAGTAAGGCAATGCAATATAGGGTATTGTTATTCCATATCTATTGCTTCCCTCCACTTCTATCATGACTCGTATACCTCAGTTATGTAATTTTGTTGGCTTACTATCTTGAGTGATTGACATGTTTTACATCTAAATGGTAGGCTCTCAGACAAAGTGTACATGCTTGCTGGTGAGGTGCTAAAGAAGTTGGCTTCAGTGATTGCCTCTCACCGTAAGTTTTTCACGCAAGAGCTTTCAGATCTTGCGCAAGATTTGAGTAGTGCAGCTGTGTCTGAGCTTATAACCCTGAAGAACACGCGAATGATGGGTCTCAGTTCTGGTTCTATGTCTGGTGCTGCTATGCTTCGAGTGTTGCAAGCACTTAGCTCACTAATTTCCCAGAGTGTTGATGGCACTAGTTGCAAAGAGAGTAACGAGGTGGAGGAGTAACCCATTTTGTTGAAGTTGAATTCTGCCCTGGAACCTTTGTGGCAGGAACTTAGCGACTGTATTAGCACAACCGAGTCAGCGTTGGGACAAAGCACTTTATCACCAATCATGGCTTCTATGAGTGTTGGAGATGGTGTTGGGGATGGTGTTGCTGGATTATCTCTCTCATCCCCCCTCTTCCTCCTGGGACACAGAGACTCTTGCCATTCATTGAGTCTTTCTTTGTTCTGTGTGAAAAGCTGCAGTCAAGCAATACATTTGTTCACCAGGATCAAACAAATGCAACTGCTCAAGAAGTTAAAGAATCTGCTGATGCTTCTTCCTCAGCACCGACAAAGTGTGGTGGTGATTCTCAGAGAAGATTTGACAGTGCTGTCATATTTTCAAGGTTTGCTGACAAACACCGTCGACTTTTGAATGCATTTATCAGGCAGAATCCAGGGTTGCTGGAAAAGTCGCTATCTATGATGCTGAAAGCACCTCGTCTCATCGATTTTGATAACAAGAGGGCATACTTTCGTTCTAGAATCAGGCAACAGCATGAGCAACACCATGCTGGGCCACTCAGAATAAGTGTTCGCCGGGCATACGTTCTTGAAGACTCATATAATCAGCTGCGAATGAGATCGAATCAGGATCTGAAGGGGCGATTGAATGTTCAGTTTCAAGGTGAAGAGGGAATTGATGCTGGTGGCCTAACAAGAGAGTGGTACCAGTTATTGTCAAGGGTTATCTTTGATAAGGGTGCACTACTGTTCACCACCGTCGGAAATGATGCAACCTTCCAGCCAAACCCTAATTCTGTTTACCAGACTGAACATCTATCATACTTCAAATTTGTTGGCCGTGTG

mRNA sequence

TGGAAGTCTTTCAGCCACCAATCCCAGAGGCTCTATGGTTGCCCCTCAAATGTGGTCTATGGTCATTCCCAGTTGTTAGAGGGTCCTCCACCCTTGGTTTTGCGTCTTGTTCTTGAGATTTTAAATTATTTGGCCACCAATCATTCTGCTGTTGCAAACACGTTGTTCTACTTTGAGCCATCACTTTTGCCCGAGTCTTTCCAGAAGTATGCAGTAAACAAAAAGGGAAAGGGCAAAGAAAAAGTAGGGGAAGCTGGAGTGCCTCCAAACTCTGTTCAGGAATACAAGGAAGGGGGTACTCCTTTAATATTGTTGTTGAAGCTTTTGAATCGCCCTCTTTTCCGTCGTAGCATTGCTCATGTTGAGCAGGTTATGGGTCTGCTTCAAGTGGTGGTCTATACTGCAGCGTCAAGATTAGAGAGCCCGTCTCTCCCTGAGCAGGTCTTAGATGATTCTCAGAATCTTCCTAGTAAAGCTTCTGATGAGGTAGAAAGAGATGTTGTTTTGGTGGAAACAGAGACTAATAAACAGAATAAAGTTGAAAATGGGGAACCCTCTATATCTGATAGGAGTACAGGTCATAATATCCATGATGTCTTCACGCAGCTGCCAAAATCTGATTTGCGTTATCTCTCCCGTCTTCTTGGCTATGAGGGGCTCTCAGACAAAGTGTACATGCTTGCTGGTGAGGTGCTAAAGAAGTTGGCTTCAGTGATTGCCTCTCACCGTAAGTTTTTCACGCAAGAGCTTTCAGATCTTGCGCAAGATTTGAGTAGTGCAGCTGTGTCTGAGCTTATAACCCTGAAGAACACGCGAATGATGGGTCTCAGTTCTGGTTCTATGTCTGGTGCTGCTATGCTTCGAGTGTTGCAAGCACTTAGCTCACTAATTTCCCAGAGTGTTGATGGCACTAGTTGCAAAGAGAGTAACGAGTCAAGCAATACATTTGTTCACCAGGATCAAACAAATGCAACTGCTCAAGAAGTTAAAGAATCTGCTGATGCTTCTTCCTCAGCACCGACAAAGTGTGGTGGTGATTCTCAGAGAAGATTTGACAGTGCTGTCATATTTTCAAGGTTTGCTGACAAACACCGTCGACTTTTGAATGCATTTATCAGGCAGAATCCAGGGTTGCTGGAAAAGTCGCTATCTATGATGCTGAAAGCACCTCGTCTCATCGATTTTGATAACAAGAGGGCATACTTTCGTTCTAGAATCAGGCAACAGCATGAGCAACACCATGCTGGGCCACTCAGAATAAGTGTTCGCCGGGCATACGTTCTTGAAGACTCATATAATCAGCTGCGAATGAGATCGAATCAGGATCTGAAGGGGCGATTGAATGTTCAGTTTCAAGGTGAAGAGGGAATTGATGCTGGTGGCCTAACAAGAGAGTGGTACCAGTTATTGTCAAGGGTTATCTTTGATAAGGGTGCACTACTGTTCACCACCGTCGGAAATGATGCAACCTTCCAGCCAAACCCTAATTCTGTTTACCAGACTGAACATCTATCATACTTCAAATTTGTTGGCCGTGTG

Coding sequence (CDS)

TGGAAGTCTTTCAGCCACCAATCCCAGAGGCTCTATGGTTGCCCCTCAAATGTGGTCTATGGTCATTCCCAGTTGTTAGAGGGTCCTCCACCCTTGGTTTTGCGTCTTGTTCTTGAGATTTTAAATTATTTGGCCACCAATCATTCTGCTGTTGCAAACACGTTGTTCTACTTTGAGCCATCACTTTTGCCCGAGTCTTTCCAGAAGTATGCAGTAAACAAAAAGGGAAAGGGCAAAGAAAAAGTAGGGGAAGCTGGAGTGCCTCCAAACTCTGTTCAGGAATACAAGGAAGGGGGTACTCCTTTAATATTGTTGTTGAAGCTTTTGAATCGCCCTCTTTTCCGTCGTAGCATTGCTCATGTTGAGCAGGTTATGGGTCTGCTTCAAGTGGTGGTCTATACTGCAGCGTCAAGATTAGAGAGCCCGTCTCTCCCTGAGCAGGTCTTAGATGATTCTCAGAATCTTCCTAGTAAAGCTTCTGATGAGGTAGAAAGAGATGTTGTTTTGGTGGAAACAGAGACTAATAAACAGAATAAAGTTGAAAATGGGGAACCCTCTATATCTGATAGGAGTACAGGTCATAATATCCATGATGTCTTCACGCAGCTGCCAAAATCTGATTTGCGTTATCTCTCCCGTCTTCTTGGCTATGAGGGGCTCTCAGACAAAGTGTACATGCTTGCTGGTGAGGTGCTAAAGAAGTTGGCTTCAGTGATTGCCTCTCACCGTAAGTTTTTCACGCAAGAGCTTTCAGATCTTGCGCAAGATTTGAGTAGTGCAGCTGTGTCTGAGCTTATAACCCTGAAGAACACGCGAATGATGGGTCTCAGTTCTGGTTCTATGTCTGGTGCTGCTATGCTTCGAGTGTTGCAAGCACTTAGCTCACTAATTTCCCAGAGTGTTGATGGCACTAGTTGCAAAGAGAGTAACGAGTCAAGCAATACATTTGTTCACCAGGATCAAACAAATGCAACTGCTCAAGAAGTTAAAGAATCTGCTGATGCTTCTTCCTCAGCACCGACAAAGTGTGGTGGTGATTCTCAGAGAAGATTTGACAGTGCTGTCATATTTTCAAGGTTTGCTGACAAACACCGTCGACTTTTGAATGCATTTATCAGGCAGAATCCAGGGTTGCTGGAAAAGTCGCTATCTATGATGCTGAAAGCACCTCGTCTCATCGATTTTGATAACAAGAGGGCATACTTTCGTTCTAGAATCAGGCAACAGCATGAGCAACACCATGCTGGGCCACTCAGAATAAGTGTTCGCCGGGCATACGTTCTTGAAGACTCATATAATCAGCTGCGAATGAGATCGAATCAGGATCTGAAGGGGCGATTGAATGTTCAGTTTCAAGGTGAAGAGGGAATTGATGCTGGTGGCCTAACAAGAGAGTGGTACCAGTTATTGTCAAGGGTTATCTTTGATAAGGGTGCACTACTGTTCACCACCGTCGGAAATGATGCAACCTTCCAGCCAAACCCTAATTCTGTTTACCAGACTGAACATCTATCATACTTCAAATTTGTTGGCCGTGTG

Protein sequence

WKSFSHQSQRLYGCPSNVVYGHSQLLEGPPPLVLRLVLEILNYLATNHSAVANTLFYFEPSLLPESFQKYAVNKKGKGKEKVGEAGVPPNSVQEYKEGGTPLILLLKLLNRPLFRRSIAHVEQVMGLLQVVVYTAASRLESPSLPEQVLDDSQNLPSKASDEVERDVVLVETETNKQNKVENGEPSISDRSTGHNIHDVFTQLPKSDLRYLSRLLGYEGLSDKVYMLAGEVLKKLASVIASHRKFFTQELSDLAQDLSSAAVSELITLKNTRMMGLSSGSMSGAAMLRVLQALSSLISQSVDGTSCKESNESSNTFVHQDQTNATAQEVKESADASSSAPTKCGGDSQRRFDSAVIFSRFADKHRRLLNAFIRQNPGLLEKSLSMMLKAPRLIDFDNKRAYFRSRIRQQHEQHHAGPLRISVRRAYVLEDSYNQLRMRSNQDLKGRLNVQFQGEEGIDAGGLTREWYQLLSRVIFDKGALLFTTVGNDATFQPNPNSVYQTEHLSYFKFVGRV

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo03048	Spo03048	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo03048.1	Spo03048.1-protein	polypeptide

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo03048.1.exon.1	Spo03048.1.exon.1	exon
Spo03048.1.exon.2	Spo03048.1.exon.2	exon
Spo03048.1.exon.3	Spo03048.1.exon.3	exon
Spo03048.1.exon.4	Spo03048.1.exon.4	exon
Spo03048.1.exon.5	Spo03048.1.exon.5	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo03048.1.CDS.1	Spo03048.1.CDS.1	CDS
Spo03048.1.CDS.2	Spo03048.1.CDS.2	CDS
Spo03048.1.CDS.3	Spo03048.1.CDS.3	CDS
Spo03048.1.CDS.4	Spo03048.1.CDS.4	CDS
Spo03048.1.CDS.5	Spo03048.1.CDS.5	CDS

Homology

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BLAST of Spo03048.1 vs. NCBI nr
Match: gi|296089933|emb|CBI39752.3| (unnamed protein product [Vitis vinifera])

HSP 1 Score: 474.2 bits (1219), Expect = 3.000e-130
Identity = 294/550 (53.45%), Postives = 355/550 (64.55%), Query Frame = 1


		  
Query: 8    SQRLYGCPSNVVYGHSQLLEGPPPLVLRLVLEILNYLATNHSAVANTLFYFEPSLLPESF 67
            SQRLYGC SNVVYG SQLL+G PP+VLR V+EIL YLATNH  VAN LFYF+PS + ES 
Sbjct: 670  SQRLYGCQSNVVYGRSQLLDGLPPVVLRRVIEILTYLATNHPVVANLLFYFDPSSVVESS 729

Query: 68   Q-KYAVNKKGKGKEKVGEAGVPPNSVQEYKEGGTPLILLLKLLNRPLFRRSIAHVEQVMG 127
              KY   KK K KEK+ E GV PN     ++G  PLIL LKLL+RP+  +SIAH++QVM 
Sbjct: 730  SPKYTETKKDKCKEKIVEGGVSPNPSGSSQQGDVPLILFLKLLDRPISLQSIAHLDQVMN 789

Query: 128  LLQVVVYTAASRLESPSLPEQVLDDSQNLPSKASDEVERDVVLVETETNKQNKVENGEPS 187
            LLQVVV +AAS+LE  +  EQ  DDSQNLP+   +E   D  L+E  +N+++K  + E S
Sbjct: 790  LLQVVVNSAASKLECQTQSEQATDDSQNLPA---NEASGDPTLLEQNSNQEDKGHSAELS 849

Query: 188  ISDRSTGHNIHDVFTQLPKSDLRYLSRLLGYEGLSDKVYMLAGEVLKKLASVIASHRKFF 247
             SD     N +D+F QLP+SDL  L  LLGYEGL DKVY  AGEVLKKLASV   HRKFF
Sbjct: 850  TSDGKKCINTYDIFLQLPQSDLHNLCSLLGYEGLPDKVYKFAGEVLKKLASVAVPHRKFF 909

Query: 248  TQELSDLAQDLSSAAVSELITLKNTRMMGLSSGSMSGAAMLRVLQALSSLISQSVDGTSC 307
            T ELSDLA  LSS+AVSEL+TL+NT M+GLS+ SM+GAA+LRVLQ LSSL S ++DG   
Sbjct: 910  TSELSDLAHHLSSSAVSELVTLRNTHMLGLSAASMAGAAILRVLQVLSSLNSPNIDGNKG 969

Query: 308  KESNESSNTFVHQDQTNATAQEV-KESADASSSAPTKCGGDSQRRFDSAVIFSRFADKHR 367
             ES+          + N   + + +E +D  S+  T+ G  S     S  +   F +   
Sbjct: 970  MESDGEPEEQTIMWKLNVALEPLWQELSDCISTTETQLGNSS----FSPTMLLPFIEAFF 1029

Query: 368  RLLNAFIRQNPGLLEKSLSMMLKAPRLID--------FDNKRAYFRSRIRQQH------- 427
             L    ++ N  ++ +  + +    R +D         +  R    + IRQ         
Sbjct: 1030 VLCEK-LQANHSVMHQDHANITARERRLDGSVTFVRFAEKHRRLLNAFIRQNPGLLEKSL 1089

Query: 428  ------------------------EQH--HAGP-LRISVRRAYVLEDSYNQLRMRSNQDL 487
                                    +QH  H    LRISVRRAYVLEDSYNQLR+R  Q+L
Sbjct: 1090 SLVLKAPRLIDFDNKRAYFRSRIRQQHEQHLSGPLRISVRRAYVLEDSYNQLRLRPTQEL 1149

Query: 488  KGRLNVQFQGEEGIDAGGLTREWYQLLSRVIFDKGALLFTTVGNDATFQPNPNSVYQTEH 514
            KGRLNVQFQGEEGIDAGGLTREWYQLLSRVIFDKGALLFTTVGN++TFQPNPNSVYQTEH
Sbjct: 1150 KGRLNVQFQGEEGIDAGGLTREWYQLLSRVIFDKGALLFTTVGNNSTFQPNPNSVYQTEH 1209

[Close]

BLAST of Spo03048.1 vs. NCBI nr
Match: gi|870861712|gb|KMT12984.1| (hypothetical protein BVRB_4g090620 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 470.7 bits (1210), Expect = 3.300e-129
Identity = 259/336 (77.08%), Postives = 286/336 (85.12%), Query Frame = 1


		  
Query: 9    QRLYGCPSNVVYGHSQLLEGPPPLVLRLVLEILNYLATNHSAVANTLFYFEPSLLPESFQ 68
            QRLYGCPSNVVYG SQLL+G PPLVLR VLEILNYLATNHSAVAN LFYFEPSLLPESFQ
Sbjct: 2672 QRLYGCPSNVVYGQSQLLDGLPPLVLRRVLEILNYLATNHSAVANILFYFEPSLLPESFQ 2731

Query: 69   KYAVNKKGKGKEKVGEAGVPPNSVQEYKEGGTPLILLLKLLNRPLFRRSIAHVEQVMGLL 128
            K+  +KKGKGKEKVGE G+P NS+ E +EG  PL+LLLKLLNRPLFRRSIAHVEQVMGLL
Sbjct: 2732 KFTESKKGKGKEKVGEIGLPQNSLGESEEGNIPLVLLLKLLNRPLFRRSIAHVEQVMGLL 2791

Query: 129  QVVVYTAASRLESPSLPEQVLDDSQNLPSKASDEVERDVVLVETETNKQNKVENGEPSIS 188
            QVVVYTAASRLES SLPEQ LDDSQN   KASDEV+RD  LVETET++QNKV N EPSIS
Sbjct: 2792 QVVVYTAASRLESVSLPEQALDDSQNQTGKASDEVQRDASLVETETSQQNKVVNREPSIS 2851

Query: 189  DRSTGHNIHDVFTQLPKSDLRYLSRLLGYEGLSDKVYMLAGEVLKKLASVIASHRKFFTQ 248
            DR  GHNI+DVFT+LP+SDLR LSRLLGYEGLSDKVYMLAGEVL+KLASV ASHRKFFTQ
Sbjct: 2852 DRKPGHNIYDVFTRLPRSDLRNLSRLLGYEGLSDKVYMLAGEVLQKLASVTASHRKFFTQ 2911

Query: 249  ELSDLAQDLSSAAVSELITLKNTRMMGLSSGSMSGAAMLRVLQALSSLISQSVDGTSCKE 308
            ELSDLAQ LSSAAVSELITLKNTRMMGLS+GSM+GAAMLRVLQALSSL+S SVD TSC+ 
Sbjct: 2912 ELSDLAQGLSSAAVSELITLKNTRMMGLSAGSMAGAAMLRVLQALSSLVSHSVDDTSCQG 2971

Query: 309  SNESSNTFVHQDQTNATAQEVKESADASSSAPTKCG 345
            S+E     +     +A     +E ++  S+  ++ G
Sbjct: 2972 SDEEEEQAILLKLNSALEALWQELSNCISTTESELG 3007

[Close]

BLAST of Spo03048.1 vs. NCBI nr
Match: gi|731330139|ref|XP_010675966.1| (PREDICTED: E3 ubiquitin-protein ligase UPL1-like isoform X2 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 470.7 bits (1210), Expect = 3.300e-129
Identity = 259/336 (77.08%), Postives = 286/336 (85.12%), Query Frame = 1


		  
Query: 9    QRLYGCPSNVVYGHSQLLEGPPPLVLRLVLEILNYLATNHSAVANTLFYFEPSLLPESFQ 68
            QRLYGCPSNVVYG SQLL+G PPLVLR VLEILNYLATNHSAVAN LFYFEPSLLPESFQ
Sbjct: 2903 QRLYGCPSNVVYGQSQLLDGLPPLVLRRVLEILNYLATNHSAVANILFYFEPSLLPESFQ 2962

Query: 69   KYAVNKKGKGKEKVGEAGVPPNSVQEYKEGGTPLILLLKLLNRPLFRRSIAHVEQVMGLL 128
            K+  +KKGKGKEKVGE G+P NS+ E +EG  PL+LLLKLLNRPLFRRSIAHVEQVMGLL
Sbjct: 2963 KFTESKKGKGKEKVGEIGLPQNSLGESEEGNIPLVLLLKLLNRPLFRRSIAHVEQVMGLL 3022

Query: 129  QVVVYTAASRLESPSLPEQVLDDSQNLPSKASDEVERDVVLVETETNKQNKVENGEPSIS 188
            QVVVYTAASRLES SLPEQ LDDSQN   KASDEV+RD  LVETET++QNKV N EPSIS
Sbjct: 3023 QVVVYTAASRLESVSLPEQALDDSQNQTGKASDEVQRDASLVETETSQQNKVVNREPSIS 3082

Query: 189  DRSTGHNIHDVFTQLPKSDLRYLSRLLGYEGLSDKVYMLAGEVLKKLASVIASHRKFFTQ 248
            DR  GHNI+DVFT+LP+SDLR LSRLLGYEGLSDKVYMLAGEVL+KLASV ASHRKFFTQ
Sbjct: 3083 DRKPGHNIYDVFTRLPRSDLRNLSRLLGYEGLSDKVYMLAGEVLQKLASVTASHRKFFTQ 3142

Query: 249  ELSDLAQDLSSAAVSELITLKNTRMMGLSSGSMSGAAMLRVLQALSSLISQSVDGTSCKE 308
            ELSDLAQ LSSAAVSELITLKNTRMMGLS+GSM+GAAMLRVLQALSSL+S SVD TSC+ 
Sbjct: 3143 ELSDLAQGLSSAAVSELITLKNTRMMGLSAGSMAGAAMLRVLQALSSLVSHSVDDTSCQG 3202

Query: 309  SNESSNTFVHQDQTNATAQEVKESADASSSAPTKCG 345
            S+E     +     +A     +E ++  S+  ++ G
Sbjct: 3203 SDEEEEQAILLKLNSALEALWQELSNCISTTESELG 3238

[Close]

BLAST of Spo03048.1 vs. NCBI nr
Match: gi|731330137|ref|XP_010675965.1| (PREDICTED: E3 ubiquitin-protein ligase UPL1-like isoform X1 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 470.7 bits (1210), Expect = 3.300e-129
Identity = 259/336 (77.08%), Postives = 286/336 (85.12%), Query Frame = 1


		  
Query: 9    QRLYGCPSNVVYGHSQLLEGPPPLVLRLVLEILNYLATNHSAVANTLFYFEPSLLPESFQ 68
            QRLYGCPSNVVYG SQLL+G PPLVLR VLEILNYLATNHSAVAN LFYFEPSLLPESFQ
Sbjct: 2904 QRLYGCPSNVVYGQSQLLDGLPPLVLRRVLEILNYLATNHSAVANILFYFEPSLLPESFQ 2963

Query: 69   KYAVNKKGKGKEKVGEAGVPPNSVQEYKEGGTPLILLLKLLNRPLFRRSIAHVEQVMGLL 128
            K+  +KKGKGKEKVGE G+P NS+ E +EG  PL+LLLKLLNRPLFRRSIAHVEQVMGLL
Sbjct: 2964 KFTESKKGKGKEKVGEIGLPQNSLGESEEGNIPLVLLLKLLNRPLFRRSIAHVEQVMGLL 3023

Query: 129  QVVVYTAASRLESPSLPEQVLDDSQNLPSKASDEVERDVVLVETETNKQNKVENGEPSIS 188
            QVVVYTAASRLES SLPEQ LDDSQN   KASDEV+RD  LVETET++QNKV N EPSIS
Sbjct: 3024 QVVVYTAASRLESVSLPEQALDDSQNQTGKASDEVQRDASLVETETSQQNKVVNREPSIS 3083

Query: 189  DRSTGHNIHDVFTQLPKSDLRYLSRLLGYEGLSDKVYMLAGEVLKKLASVIASHRKFFTQ 248
            DR  GHNI+DVFT+LP+SDLR LSRLLGYEGLSDKVYMLAGEVL+KLASV ASHRKFFTQ
Sbjct: 3084 DRKPGHNIYDVFTRLPRSDLRNLSRLLGYEGLSDKVYMLAGEVLQKLASVTASHRKFFTQ 3143

Query: 249  ELSDLAQDLSSAAVSELITLKNTRMMGLSSGSMSGAAMLRVLQALSSLISQSVDGTSCKE 308
            ELSDLAQ LSSAAVSELITLKNTRMMGLS+GSM+GAAMLRVLQALSSL+S SVD TSC+ 
Sbjct: 3144 ELSDLAQGLSSAAVSELITLKNTRMMGLSAGSMAGAAMLRVLQALSSLVSHSVDDTSCQG 3203

Query: 309  SNESSNTFVHQDQTNATAQEVKESADASSSAPTKCG 345
            S+E     +     +A     +E ++  S+  ++ G
Sbjct: 3204 SDEEEEQAILLKLNSALEALWQELSNCISTTESELG 3239

[Close]

BLAST of Spo03048.1 vs. NCBI nr
Match: gi|659096357|ref|XP_008449051.1| (PREDICTED: E3 ubiquitin-protein ligase UPL1 isoform X2 [Cucumis melo])

HSP 1 Score: 443.7 bits (1140), Expect = 4.300e-121
Identity = 292/586 (49.83%), Postives = 348/586 (59.39%), Query Frame = 1


		  
Query: 8    SQRLYGCPSNVVYGHSQLLEGPPPLVLRLVLEILNYLATNHSAVANTLFYFEPSLLPESF 67
            SQRLYGC SNVVYG SQLL+G PPL+LR +LEIL YLATNHSAVAN LFYF+   +PE  
Sbjct: 2935 SQRLYGCQSNVVYGRSQLLDGLPPLMLRRILEILTYLATNHSAVANMLFYFDLETVPEDL 2994

Query: 68   QKYAVNKKGKGKEKVGEAGVPPNSVQEYKEGGTPLILLLKLLNRPLFRRSIAHVEQVMGL 127
                +  K KGKEKV E G+P + ++  +    PL+  LKLLNRPLF RS+ H+EQV+ L
Sbjct: 2995 SSSCMETK-KGKEKVVE-GLPTSKLKTCQAVNIPLVQFLKLLNRPLFLRSVVHLEQVVSL 3054

Query: 128  LQVVVYTAASRLESPSLPEQVLDDSQNLP-SKASDEVERDVVLVETETNKQNKVENGEPS 187
            LQVVVYTA+S+LE  S  EQV  +S  LP  +AS  V +D  L E ++ + N    G  S
Sbjct: 3055 LQVVVYTASSKLEHQSRSEQVTGNSPMLPVDEASGAVSKDPSLPEGDSKQDNSDAAG--S 3114

Query: 188  ISDRSTGHNIHDVFTQLPKSDLRYLSRLLGYEGLSDKVYMLAGEVLKKLASVIASHRKFF 247
             S     ++IH++F QLP S L  L  LLG EGLSDKVY LAGEVLKKL+SV A HRKFF
Sbjct: 3115 TSGGKGSNDIHNIFLQLPHSVLCNLCALLGREGLSDKVYTLAGEVLKKLSSVAAPHRKFF 3174

Query: 248  TQELSDLAQDLSSAAVSELITLKNTRMMGLSSGSMSGAAMLRVLQALSSLISQSVDGTSC 307
              ELS+LA  LSS+A+SEL+TL+NT M+GLS+ SM+GAA++RVLQALSSL S  V  T  
Sbjct: 3175 MSELSELANGLSSSAISELVTLRNTNMLGLSASSMAGAAIVRVLQALSSLTSPCVRETKS 3234

Query: 308  KESNES---------------------------SNTFVHQDQTNATAQEVKESADAS-SS 367
             E +                             + T + Q  T+ T   V E    + SS
Sbjct: 3235 SECDSELEEQAIMWRLNVALEPLWQALSDCISVTETQLSQSSTSTTPVNVGEQLQGTISS 3294

Query: 368  APTKCGGDSQRRFDSA-------------VIFSRFADKHRRLLNAFIRQNPGLLEKSLSM 427
            +P   GG     F  A             ++    A+   R +  F   +  L  K    
Sbjct: 3295 SPLPPGGQRLLPFIEAFFVLSEKLQANLSILQQDHANVTAREVKEFAGTSDTLSTKGADF 3354

Query: 428  MLKAPRLIDF----DNKRAYFRSRIRQQH------------------------------- 487
              K+   + F    +  R    + IRQ                                 
Sbjct: 3355 QKKSDGAVTFTRFAERHRRLLNAFIRQNPGLMEKSLSILLKAPRLIDFDNKRAYFRSRIR 3414

Query: 488  --EQHHAGP-LRISVRRAYVLEDSYNQLRMRSNQDLKGRLNVQFQGEEGIDAGGLTREWY 514
               + H    LRISVRRAYVLEDSYNQLRMR  QDL+GRLNVQFQGEEGIDAGGLTREWY
Sbjct: 3415 QQNEQHISGPLRISVRRAYVLEDSYNQLRMRPTQDLRGRLNVQFQGEEGIDAGGLTREWY 3474

[Close]

BLAST of Spo03048.1 vs. UniProtKB/TrEMBL
Match: A0A0J8CLT3_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_4g090620 PE=4 SV=1)

HSP 1 Score: 470.7 bits (1210), Expect = 2.300e-129
Identity = 259/336 (77.08%), Postives = 286/336 (85.12%), Query Frame = 1


		  
Query: 9    QRLYGCPSNVVYGHSQLLEGPPPLVLRLVLEILNYLATNHSAVANTLFYFEPSLLPESFQ 68
            QRLYGCPSNVVYG SQLL+G PPLVLR VLEILNYLATNHSAVAN LFYFEPSLLPESFQ
Sbjct: 2672 QRLYGCPSNVVYGQSQLLDGLPPLVLRRVLEILNYLATNHSAVANILFYFEPSLLPESFQ 2731

Query: 69   KYAVNKKGKGKEKVGEAGVPPNSVQEYKEGGTPLILLLKLLNRPLFRRSIAHVEQVMGLL 128
            K+  +KKGKGKEKVGE G+P NS+ E +EG  PL+LLLKLLNRPLFRRSIAHVEQVMGLL
Sbjct: 2732 KFTESKKGKGKEKVGEIGLPQNSLGESEEGNIPLVLLLKLLNRPLFRRSIAHVEQVMGLL 2791

Query: 129  QVVVYTAASRLESPSLPEQVLDDSQNLPSKASDEVERDVVLVETETNKQNKVENGEPSIS 188
            QVVVYTAASRLES SLPEQ LDDSQN   KASDEV+RD  LVETET++QNKV N EPSIS
Sbjct: 2792 QVVVYTAASRLESVSLPEQALDDSQNQTGKASDEVQRDASLVETETSQQNKVVNREPSIS 2851

Query: 189  DRSTGHNIHDVFTQLPKSDLRYLSRLLGYEGLSDKVYMLAGEVLKKLASVIASHRKFFTQ 248
            DR  GHNI+DVFT+LP+SDLR LSRLLGYEGLSDKVYMLAGEVL+KLASV ASHRKFFTQ
Sbjct: 2852 DRKPGHNIYDVFTRLPRSDLRNLSRLLGYEGLSDKVYMLAGEVLQKLASVTASHRKFFTQ 2911

Query: 249  ELSDLAQDLSSAAVSELITLKNTRMMGLSSGSMSGAAMLRVLQALSSLISQSVDGTSCKE 308
            ELSDLAQ LSSAAVSELITLKNTRMMGLS+GSM+GAAMLRVLQALSSL+S SVD TSC+ 
Sbjct: 2912 ELSDLAQGLSSAAVSELITLKNTRMMGLSAGSMAGAAMLRVLQALSSLVSHSVDDTSCQG 2971

Query: 309  SNESSNTFVHQDQTNATAQEVKESADASSSAPTKCG 345
            S+E     +     +A     +E ++  S+  ++ G
Sbjct: 2972 SDEEEEQAILLKLNSALEALWQELSNCISTTESELG 3007

[Close]

BLAST of Spo03048.1 vs. UniProtKB/TrEMBL
Match: A0A0A0L640_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_3G047920 PE=4 SV=1)

HSP 1 Score: 425.6 bits (1093), Expect = 8.400e-116
Identity = 285/587 (48.55%), Postives = 345/587 (58.77%), Query Frame = 1


		  
Query: 8    SQRLYGCPSNVVYGHSQLLEGPPPLVLRLVLEILNYLATNHSAVANTLFYFEPSLLPESF 67
            SQRLYGC SNVVYG SQLL+G PPL+LR +LEIL YLATNHSAVAN LFYF+   +PE  
Sbjct: 2936 SQRLYGCQSNVVYGRSQLLDGLPPLMLRRILEILTYLATNHSAVANMLFYFDLETVPEDL 2995

Query: 68   QKYAVNKKGKGKEKVGEAGVPPNSVQEYKEGGTPLILLLKLLNRPLFRRSIAHVEQVMGL 127
                +  K KGKEKV E G+P ++++  +    PL+  LKLLNRPLF RS+ H+EQV+ L
Sbjct: 2996 SSSCMETK-KGKEKVVE-GLPASNLKTCQAVNIPLVQFLKLLNRPLFLRSVVHLEQVVSL 3055

Query: 128  LQVVVYTAASRLESPSLPEQVLDDSQNLP-SKASDEVERDVVLVETETNKQNKVENGEPS 187
            LQVVVYTA+S+LE  S  EQV  +S  LP  +AS  V +D  L E ++ + N    G  S
Sbjct: 3056 LQVVVYTASSKLEHQSRSEQVTGNSPMLPVDEASGAVSKDPSLPEGDSKQDNSDAAG--S 3115

Query: 188  ISDRSTGHNIHDVFTQLPKSDLRYLSRLLGYEGLSDKVYMLAGEVLKKLASVIASHRKFF 247
             S     ++IH++F QLP S L  L  LLG EGLSDKVY LAGEVLKKL+SV A HRKFF
Sbjct: 3116 TSGGKGSNDIHNIFLQLPHSVLCNLCALLGREGLSDKVYTLAGEVLKKLSSVAAPHRKFF 3175

Query: 248  TQELSDLAQDLSSAAVSELITLKNTRMMGLSSGSMSGAAMLRVLQALSSLISQSVDGTSC 307
              ELS+LA  LSS+A+SEL+TLKNT M+GLS+ SM+GAA++RVLQALSSL S  V  T  
Sbjct: 3176 MSELSELANGLSSSAISELVTLKNTNMLGLSASSMAGAAIVRVLQALSSLTSPCVRETKS 3235

Query: 308  KESNES---------------------------SNTFVHQDQTNATAQEVKESADAS-SS 367
             E +                             + T + Q  ++ T   V E    + SS
Sbjct: 3236 SECDSELEEQAIMWRLNVALEPLWQALSDCISVTETQLSQSSSSTTPINVGEQLQGTISS 3295

Query: 368  APTKCGGDSQRRFDSAVI---------FSRFADKHRRLLNAFIRQNPG----LLEKSLSM 427
            +P   GG     F  A            S     H  +    +++  G    L  K    
Sbjct: 3296 SPLPPGGQRLLPFIEAFFVLSEKLQANLSILQQDHANITAREVKEFSGTSDTLSTKGADY 3355

Query: 428  MLKAPRLIDFDNKRAYFRSRIRQQHEQHHAGPLRISV--------------RRA------ 487
              K+   + F  + A    R+     + + G +  S+              +RA      
Sbjct: 3356 QKKSDGAVTF-TRFAERHRRLLNAFIRQNPGLMEKSLSILLKAPRLIDFDNKRAYFRSRI 3415

Query: 488  -------------------YVLEDSYNQLRMRSNQDLKGRLNVQFQGEEGIDAGGLTREW 514
                               YVLEDSYNQLRMR  QDL+GRLNVQFQGEEGIDAGGLTREW
Sbjct: 3416 RQQNEQHISGPLRISVRRAYVLEDSYNQLRMRPTQDLRGRLNVQFQGEEGIDAGGLTREW 3475

[Close]

BLAST of Spo03048.1 vs. UniProtKB/TrEMBL
Match: A0A0K9Q6X1_SPIOL (Uncharacterized protein (Fragment) OS=Spinacia oleracea GN=SOVF_214860 PE=4 SV=1)

HSP 1 Score: 409.5 bits (1051), Expect = 6.200e-111
Identity = 209/211 (99.05%), Postives = 209/211 (99.05%), Query Frame = 1


		  
Query: 9   QRLYGCPSNVVYGHSQLLEGPPPLVLRLVLEILNYLATNHSAVANTLFYFEPSLLPESFQ 68
           QRLYGCPSNVVYGHSQLLEGPPPLVLRLVLEILNYLATNHSAVANTLFYFEPSLLPESFQ
Sbjct: 1   QRLYGCPSNVVYGHSQLLEGPPPLVLRLVLEILNYLATNHSAVANTLFYFEPSLLPESFQ 60

Query: 69  KYAVNKKGKGKEKVGEAGVPPNSVQEYKEGGTPLILLLKLLNRPLFRRSIAHVEQVMGLL 128
           KYAVNKKGKGKEKVGEAGVPPNSVQEYKEGGTPLILLLKLLNRPLFRRSIAHVEQVMGLL
Sbjct: 61  KYAVNKKGKGKEKVGEAGVPPNSVQEYKEGGTPLILLLKLLNRPLFRRSIAHVEQVMGLL 120

Query: 129 QVVVYTAASRLESPSLPEQVLDDSQNLPSKASDEVERDVVLVETETNKQNKVENGEPSIS 188
           QVVVYTAASRLESPSLPEQVLDDSQNLPSKASDEVERDV LVETETNKQNKVENGEPSIS
Sbjct: 121 QVVVYTAASRLESPSLPEQVLDDSQNLPSKASDEVERDVALVETETNKQNKVENGEPSIS 180

Query: 189 DRSTGHNIHDVFTQLPKSDLRYLSRLLGYEG 220
           DRSTGHNIHDVFTQ PKSDLRYLSRLLGYEG
Sbjct: 181 DRSTGHNIHDVFTQQPKSDLRYLSRLLGYEG 211

[Close]

BLAST of Spo03048.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RY40_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_020560 PE=4 SV=1)

HSP 1 Score: 345.5 bits (885), Expect = 1.100e-91
Identity = 180/190 (94.74%), Postives = 181/190 (95.26%), Query Frame = 1


		  
Query: 9    QRLYGCPSNVVYGHSQLLEGPPPLVLRLVLEILNYLATNHSAVANTLFYFEPSLLPESFQ 68
            QRLYGCPSNVVYGHSQLLEG PPLVLR VLEILNYLATNHSAVANTLFYFEPSLLPESFQ
Sbjct: 2805 QRLYGCPSNVVYGHSQLLEGLPPLVLRRVLEILNYLATNHSAVANTLFYFEPSLLPESFQ 2864

Query: 69   KYAVNKKGKGKEKVGEAGVPPNSVQEYKEGGTPLILLLKLLNRPLFRRSIAHVEQVMGLL 128
            KYAVNKKGKGKEKVGE GVPPNS QEYKEGGTPLILLLKLLNRPLFRRSIAHVEQVMGLL
Sbjct: 2865 KYAVNKKGKGKEKVGEGGVPPNSDQEYKEGGTPLILLLKLLNRPLFRRSIAHVEQVMGLL 2924

Query: 129  QVVVYTAASRLESPSLPEQVLDDSQNLPSKASDEVERDVVLVETETNKQNKVENGEPSIS 188
            QVVVYTAASRLESPSLPEQVLDDSQNLPSKASDEVERDV LVETETNKQNKVENGEPSIS
Sbjct: 2925 QVVVYTAASRLESPSLPEQVLDDSQNLPSKASDEVERDVALVETETNKQNKVENGEPSIS 2984

Query: 189  DRSTGHNIHD 199
            DRST   + D
Sbjct: 2985 DRSTAPRLID 2994

[Close]

BLAST of Spo03048.1 vs. UniProtKB/TrEMBL
Match: A0A0R0K000_SOYBN (Uncharacterized protein (Fragment) OS=Glycine max GN=GLYMA_05G1331001 PE=4 SV=1)

HSP 1 Score: 342.8 bits (878), Expect = 7.100e-91
Identity = 172/206 (83.50%), Postives = 187/206 (90.78%), Query Frame = 1


		  
Query: 308  ESNESSNTFVHQDQTNATAQEVKESADASSSAPTKCGGDSQRRFDSAVIFSRFADKHRRL 367
            E  +++ +F+ QD  NATA+EVKESA  S+S   K GGDS R+FD A+ F+RFA+KHRRL
Sbjct: 3279 EKLQANESFMQQDHCNATAREVKESAGCSASTSVKIGGDSLRKFDGAITFTRFAEKHRRL 3338

Query: 368  LNAFIRQNPGLLEKSLSMMLKAPRLIDFDNKRAYFRSRIRQQHEQHHAGPLRISVRRAYV 427
             NAFIRQNPGLLEKSLSMMLKAPRLIDFDNKRAYFRSRIRQQH+QH +GPLRISVRRAY+
Sbjct: 3339 SNAFIRQNPGLLEKSLSMMLKAPRLIDFDNKRAYFRSRIRQQHDQHLSGPLRISVRRAYI 3398

Query: 428  LEDSYNQLRMRSNQDLKGRLNVQFQGEEGIDAGGLTREWYQLLSRVIFDKGALLFTTVGN 487
            LEDSYNQLRMR  QDLKGRLNVQFQGEEGIDAGGLTREWYQLLSRVIFDKGALLFTTVGN
Sbjct: 3399 LEDSYNQLRMRPTQDLKGRLNVQFQGEEGIDAGGLTREWYQLLSRVIFDKGALLFTTVGN 3458

Query: 488  DATFQPNPNSVYQTEHLSYFKFVGRV 514
            +ATFQPNPNSVYQTEHLSYFKFVGRV
Sbjct: 3459 NATFQPNPNSVYQTEHLSYFKFVGRV 3484

[Close]

BLAST of Spo03048.1 vs. ExPASy Swiss-Prot
Match: UPL1_ARATH (E3 ubiquitin-protein ligase UPL1 OS=Arabidopsis thaliana GN=UPL1 PE=1 SV=3)

HSP 1 Score: 320.5 bits (820), Expect = 3.400e-86
Identity = 164/195 (84.10%), Postives = 176/195 (90.26%), Query Frame = 1


		  
Query: 319  QDQTNATAQEVKESADASSSAPTKCGGDSQRRFDSAVIFSRFADKHRRLLNAFIRQNPGL 378
            Q  TN TA EVKES+   SS+ T    DSQ++ D +V FS+FA+KHRRLLN+FIRQNP L
Sbjct: 3222 QQDTNVTAGEVKESSAHGSSSKTSV--DSQKKTDGSVTFSKFAEKHRRLLNSFIRQNPSL 3281

Query: 379  LEKSLSMMLKAPRLIDFDNKRAYFRSRIRQQHEQHHAGPLRISVRRAYVLEDSYNQLRMR 438
            LEKSLSMMLKAPRLIDFDNK+AYFRSRIR QH+QH +GPLRISVRRAYVLEDSYNQLRMR
Sbjct: 3282 LEKSLSMMLKAPRLIDFDNKKAYFRSRIRHQHDQHISGPLRISVRRAYVLEDSYNQLRMR 3341

Query: 439  SNQDLKGRLNVQFQGEEGIDAGGLTREWYQLLSRVIFDKGALLFTTVGNDATFQPNPNSV 498
            S QDLKGRLNVQFQGEEGIDAGGLTREWYQLLSRVIFDKGALLFTTVGNDATFQPNPNSV
Sbjct: 3342 SPQDLKGRLNVQFQGEEGIDAGGLTREWYQLLSRVIFDKGALLFTTVGNDATFQPNPNSV 3401

Query: 499  YQTEHLSYFKFVGRV 514
            YQTEHLSYFKFVGR+
Sbjct: 3402 YQTEHLSYFKFVGRM 3414

[Close]

BLAST of Spo03048.1 vs. ExPASy Swiss-Prot
Match: UPL2_ARATH (E3 ubiquitin-protein ligase UPL2 OS=Arabidopsis thaliana GN=UPL2 PE=1 SV=3)

HSP 1 Score: 312.8 bits (800), Expect = 7.100e-84
Identity = 159/195 (81.54%), Postives = 172/195 (88.21%), Query Frame = 1


		  
Query: 319  QDQTNATAQEVKESADASSSAPTKCGGDSQRRFDSAVIFSRFADKHRRLLNAFIRQNPGL 378
            Q     TA EVKES+   SS+ T    DSQ++ D +V FS+F +KHRRLLN+F+RQNP L
Sbjct: 3199 QQDATVTAGEVKESSTHGSSSKTIV--DSQKKIDGSVTFSKFVEKHRRLLNSFVRQNPSL 3258

Query: 379  LEKSLSMMLKAPRLIDFDNKRAYFRSRIRQQHEQHHAGPLRISVRRAYVLEDSYNQLRMR 438
            LEKS SMMLKAPRLIDFDNK+AYFRSRIR QH+QH +GPLRISVRRAYVLEDSYNQLRMR
Sbjct: 3259 LEKSFSMMLKAPRLIDFDNKKAYFRSRIRHQHDQHISGPLRISVRRAYVLEDSYNQLRMR 3318

Query: 439  SNQDLKGRLNVQFQGEEGIDAGGLTREWYQLLSRVIFDKGALLFTTVGNDATFQPNPNSV 498
            S QDLKGRLNVQFQGEEGIDAGGLTREWYQLLSRVIFDKGALLFTTVGNDATFQPNPNSV
Sbjct: 3319 SPQDLKGRLNVQFQGEEGIDAGGLTREWYQLLSRVIFDKGALLFTTVGNDATFQPNPNSV 3378

Query: 499  YQTEHLSYFKFVGRV 514
            YQTEHLSYFKFVGR+
Sbjct: 3379 YQTEHLSYFKFVGRM 3391

[Close]

BLAST of Spo03048.1 vs. ExPASy Swiss-Prot
Match: TOM1_NEUCR (E3 ubiquitin-protein ligase TOM1-like OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) GN=B11B22.010 PE=3 SV=4)

HSP 1 Score: 158.3 bits (399), Expect = 2.200e-37
Identity = 75/164 (45.73%), Postives = 112/164 (68.29%), Query Frame = 1


		  
Query: 354  AVIFSRFADKHRRLLNAFIRQNPGLLEKSLSMMLKAPRLIDFDNKRAYFRSRIRQQHEQ- 413
            A +F  F ++HRR+LN  +R NP L+  + S+++K P++++FDNKR YF   +  +++Q 
Sbjct: 3653 AGLFFTFTEEHRRILNELVRHNPKLMSGTFSLLVKNPKVLEFDNKRNYFNRSVHSKYQQT 3712

Query: 414  -HHAGPLRISVRRAYVLEDSYNQLRMRSNQDLK-GRLNVQFQGEEGIDAGGLTREWYQLL 473
             H   PL++ VRR +V  DS+  L  +   +LK G+LN++FQGEEG+DAGG+TREW+Q+L
Sbjct: 3713 RHSFPPLQLQVRREHVFHDSFRSLYYKKADELKFGKLNIRFQGEEGVDAGGVTREWFQVL 3772

Query: 474  SRVIFDKGALLFTTVGND-ATFQPNPNSVYQTEHLSYFKFVGRV 514
            SR +FD   +LF  V +D  TF PN  S    EHL +FKF+GR+
Sbjct: 3773 SRQMFDPNYVLFVPVSSDRTTFHPNKLSPINDEHLPFFKFIGRI 3816

[Close]

BLAST of Spo03048.1 vs. ExPASy Swiss-Prot
Match: TOM1_ASHGO (Probable E3 ubiquitin-protein ligase TOM1 OS=Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) GN=TOM1 PE=3 SV=2)

HSP 1 Score: 151.0 bits (380), Expect = 3.600e-35
Identity = 76/160 (47.50%), Postives = 106/160 (66.25%), Query Frame = 1


		  
Query: 356  IFSRFADKHRRLLNAFIRQNPGLLEKSLSMMLKAPRLIDFDNKRAYFRSRIRQQHEQHHA 415
            +F  F D H++LLN  IR NP L+    S+++K P+++DFDNKR YF +++R     H  
Sbjct: 2841 LFFAFTDLHKKLLNEMIRSNPKLMSGPFSLLVKNPKILDFDNKRYYFTAQLRA--ITHDR 2900

Query: 416  GPLRISVRRAYVLEDSYNQLRMRSNQDLK-GRLNVQFQGEEGIDAGGLTREWYQLLSRVI 475
              L ISVRR +V  DSY  L  +SN+D+K  +L + F+GE G+DAGG+TREWYQ+LSR +
Sbjct: 2901 PKLSISVRREHVFLDSYRSLFFKSNEDIKISKLEISFKGEAGVDAGGITREWYQVLSRQM 2960

Query: 476  FDKGALLFTTVGND-ATFQPNPNSVYQTEHLSYFKFVGRV 514
            F+    LF  V +D  TF+PN  S    EHLS+FKF+G +
Sbjct: 2961 FNPDYALFIPVASDKTTFRPNRTSGINPEHLSFFKFIGMI 2998

[Close]

BLAST of Spo03048.1 vs. ExPASy Swiss-Prot
Match: TOM1_YEAST (E3 ubiquitin-protein ligase TOM1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TOM1 PE=1 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 2.800e-32
Identity = 69/160 (43.12%), Postives = 105/160 (65.62%), Query Frame = 1


		  
Query: 356  IFSRFADKHRRLLNAFIRQNPGLLEKSLSMMLKAPRLIDFDNKRAYFRSRIRQQHEQHHA 415
            +F  F D H++LLN  IR NP L+    ++++K P+++DFDNKR +F ++++  +++   
Sbjct: 2851 LFFPFTDAHKKLLNQMIRSNPKLMSGPFALLVKNPKVLDFDNKRYFFNAKLKSDNQERPK 2910

Query: 416  GPLRISVRRAYVLEDSYNQLRMRSNQDLK-GRLNVQFQGEEGIDAGGLTREWYQLLSRVI 475
             P  I+VRR  V  DSY  L  ++N ++K  +L + F+GE G+DAGG+TREWYQ+LSR +
Sbjct: 2911 LP--ITVRREQVFLDSYRALFFKTNDEIKNSKLEITFKGESGVDAGGVTREWYQVLSRQM 2970

Query: 476  FDKGALLFTTVGND-ATFQPNPNSVYQTEHLSYFKFVGRV 514
            F+    LF  V +D  TF PN  S    EHLS+FKF+G +
Sbjct: 2971 FNPDYALFLPVPSDKTTFHPNRTSGINPEHLSFFKFIGMI 3008

[Close]

BLAST of Spo03048.1 vs. TAIR (Arabidopsis)
Match: AT1G55860.1 (ubiquitin-protein ligase 1)

HSP 1 Score: 320.5 bits (820), Expect = 1.900e-87
Identity = 164/195 (84.10%), Postives = 176/195 (90.26%), Query Frame = 1


		  
Query: 319  QDQTNATAQEVKESADASSSAPTKCGGDSQRRFDSAVIFSRFADKHRRLLNAFIRQNPGL 378
            Q  TN TA EVKES+   SS+ T    DSQ++ D +V FS+FA+KHRRLLN+FIRQNP L
Sbjct: 3471 QQDTNVTAGEVKESSAHGSSSKTSV--DSQKKTDGSVTFSKFAEKHRRLLNSFIRQNPSL 3530

Query: 379  LEKSLSMMLKAPRLIDFDNKRAYFRSRIRQQHEQHHAGPLRISVRRAYVLEDSYNQLRMR 438
            LEKSLSMMLKAPRLIDFDNK+AYFRSRIR QH+QH +GPLRISVRRAYVLEDSYNQLRMR
Sbjct: 3531 LEKSLSMMLKAPRLIDFDNKKAYFRSRIRHQHDQHISGPLRISVRRAYVLEDSYNQLRMR 3590

Query: 439  SNQDLKGRLNVQFQGEEGIDAGGLTREWYQLLSRVIFDKGALLFTTVGNDATFQPNPNSV 498
            S QDLKGRLNVQFQGEEGIDAGGLTREWYQLLSRVIFDKGALLFTTVGNDATFQPNPNSV
Sbjct: 3591 SPQDLKGRLNVQFQGEEGIDAGGLTREWYQLLSRVIFDKGALLFTTVGNDATFQPNPNSV 3650

Query: 499  YQTEHLSYFKFVGRV 514
            YQTEHLSYFKFVGR+
Sbjct: 3651 YQTEHLSYFKFVGRM 3663

[Close]

BLAST of Spo03048.1 vs. TAIR (Arabidopsis)
Match: AT1G70320.1 (ubiquitin-protein ligase 2)

HSP 1 Score: 312.8 bits (800), Expect = 4.000e-85
Identity = 159/195 (81.54%), Postives = 172/195 (88.21%), Query Frame = 1


		  
Query: 319  QDQTNATAQEVKESADASSSAPTKCGGDSQRRFDSAVIFSRFADKHRRLLNAFIRQNPGL 378
            Q     TA EVKES+   SS+ T    DSQ++ D +V FS+F +KHRRLLN+F+RQNP L
Sbjct: 3199 QQDATVTAGEVKESSTHGSSSKTIV--DSQKKIDGSVTFSKFVEKHRRLLNSFVRQNPSL 3258

Query: 379  LEKSLSMMLKAPRLIDFDNKRAYFRSRIRQQHEQHHAGPLRISVRRAYVLEDSYNQLRMR 438
            LEKS SMMLKAPRLIDFDNK+AYFRSRIR QH+QH +GPLRISVRRAYVLEDSYNQLRMR
Sbjct: 3259 LEKSFSMMLKAPRLIDFDNKKAYFRSRIRHQHDQHISGPLRISVRRAYVLEDSYNQLRMR 3318

Query: 439  SNQDLKGRLNVQFQGEEGIDAGGLTREWYQLLSRVIFDKGALLFTTVGNDATFQPNPNSV 498
            S QDLKGRLNVQFQGEEGIDAGGLTREWYQLLSRVIFDKGALLFTTVGNDATFQPNPNSV
Sbjct: 3319 SPQDLKGRLNVQFQGEEGIDAGGLTREWYQLLSRVIFDKGALLFTTVGNDATFQPNPNSV 3378

Query: 499  YQTEHLSYFKFVGRV 514
            YQTEHLSYFKFVGR+
Sbjct: 3379 YQTEHLSYFKFVGRM 3391

[Close]

BLAST of Spo03048.1 vs. TAIR (Arabidopsis)
Match: AT3G17205.1 (ubiquitin protein ligase 6)

HSP 1 Score: 70.5 bits (171), Expect = 3.500e-12
Identity = 42/137 (30.66%), Postives = 72/137 (52.55%), Query Frame = 1


		  
Query: 385 MMLKAPRLIDFDNKRAYFRSRIRQQHEQHHAGPL----RISVRRAYVLEDSYNQLRMRSN 444
           ++++AP LI F ++   F +++    + H +  +    R  +RR ++LED+YNQ+   S 
Sbjct: 630 ILMQAPFLIPFTSRVKIFTTQLATARQSHGSQGIFARNRFRIRRDHILEDAYNQMSALSE 689

Query: 445 QDLKGRLNVQFQG-----EEGIDAGGLTREWYQLLSRVIFDKGALLFTTVGNDATFQPNP 504
            DL+  + V F       E GID GG+ +++ + ++R  FD    LF     D    PNP
Sbjct: 690 DDLRSSIRVTFVNELGVEEAGIDGGGIFKDFMEKITRAAFDVQYGLFKETA-DHMLYPNP 749

Query: 505 NS-VYQTEHLSYFKFVG 512
            S +   +HL +F F+G
Sbjct: 750 GSGMIHEQHLQFFHFLG 765

The following BLAST results are available for this feature:

BLAST of Spo03048.1 vs. NCBI nr
BLAST of Spo03048.1 vs. UniProtKB/TrEMBL
BLAST of Spo03048.1 vs. ExPASy Swiss-Prot
BLAST of Spo03048.1 vs. TAIR (Arabidopsis)

BLAST of Spo03048.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|296089933\|emb\|CBI39752.3\|	3.0e-130	53.4	unnamed protein product [Vitis...	[more]
gi\|870861712\|gb\|KMT12984.1\|	3.3e-129	77.0	hypothetical protein BVRB_4g09...	[more]
gi\|731330139\|ref\|XP_010675966.1\|	3.3e-129	77.0	PREDICTED: E3 ubiquitin-protei...	[more]
gi\|731330137\|ref\|XP_010675965.1\|	3.3e-129	77.0	PREDICTED: E3 ubiquitin-protei...	[more]
gi\|659096357\|ref\|XP_008449051.1\|	4.3e-121	49.8	PREDICTED: E3 ubiquitin-protei...	[more]

BLAST of Spo03048.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0J8CLT3_BETVU	2.3e-129	77.0	Uncharacterized protein OS=Bet...	[more]
A0A0A0L640_CUCSA	8.4e-116	48.5	Uncharacterized protein OS=Cuc...	[more]
A0A0K9Q6X1_SPIOL	6.2e-111	99.0	Uncharacterized protein (Fragm...	[more]
A0A0K9RY40_SPIOL	1.1e-91	94.7	Uncharacterized protein OS=Spi...	[more]
A0A0R0K000_SOYBN	7.1e-91	83.5	Uncharacterized protein (Fragm...	[more]

BLAST of Spo03048.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
UPL1_ARATH	3.4e-86	84.1	E3 ubiquitin-protein ligase UP...	[more]
UPL2_ARATH	7.1e-84	81.5	E3 ubiquitin-protein ligase UP...	[more]
TOM1_NEUCR	2.2e-37	45.7	E3 ubiquitin-protein ligase TO...	[more]
TOM1_ASHGO	3.6e-35	47.5	Probable E3 ubiquitin-protein ...	[more]
TOM1_YEAST	2.8e-32	43.1	E3 ubiquitin-protein ligase TO...	[more]

BLAST of Spo03048.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 3


Match Name	E-value	Identity	Description
AT1G55860.1	1.9e-87	84.1	ubiquitin-protein ligase 1	[more]
AT1G70320.1	4.0e-85	81.5	ubiquitin-protein ligase 2	[more]
AT3G17205.1	3.5e-12	30.6	ubiquitin protein ligase 6	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000569	HECT domain	PROFILE	PS50237	HECT	coord: 439..513 score: 25
IPR000569	HECT domain	unknown	SSF56204	Hect, E3 ligase catalytic domain	coord: 399..513 score: 1.96
None	No IPR available	PANTHER	PTHR11254	HECT DOMAIN UBIQUITIN-PROTEIN LIGASE	coord: 7..513 score: 3.4E
None	No IPR available	PANTHER	PTHR11254:SF291	E3 UBIQUITIN-PROTEIN LIGASE HUWE1	coord: 7..513 score: 3.4E

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
molecular_function	GO:0004842	ubiquitin-protein transferase activity
molecular_function	GO:0016874	ligase activity