Spo03335.1 (mRNA) | SpinachBase

Overview


Name	Spo03335.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	4-hydroxy-3-methylbut-2-enyl diphosphate reductase
Location	SpoScf_01182 : 85630 .. 89918 (+)
Sequence length	1748

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

GTCGGGTTGGGTTGGGAAAATCTCAACCCGTTTATAAATGGGTCGACCTGATTTCGACCCAACCTAACCCATTGCCAGCCTCTAGGATTGATGGTGTAAGCGGATCGTGGGTAACCCGACTCATTGGCCATCGACCGATAAAGACGCTAGAATCCACAATTGGCAACCCATGTGTTTGGTGAGAAATGATTTTCTAATCACACAACAAATTACAACTTATTTATAAACGGACGACCAAATTAAAATACTCAATATTATTAACACACGGATAAAATTCCCGAAAATCCAAAACTTCATTCATACCCACTGAACTCCTGTAACCTTACAACTCACGTGTCTCTCTCTCCTCTTAGCTTCAATCAGTATCAAAAATGGCGATTTCTCCTCATCTCAGCCGCATCATCACCTTCACCGAACTCCGGTTGCCGGAAACTCAATTCCGCCCAAACTTCCACAGGAAGCCTCACCTCTCCGTCCGATGTGTCGCCGGTGAGTCTCCGACGTCTTCATCATCCTCCGCCGTTGATTCCAAATTTGATGCTAAGAAGTTCCATCACAACTTGACGAGGAGTGAGAATTATAATCGCAAGGGTTTTGGACACAAAGAAGAGACTCTCCAGCTTATGAATAGCGAGTATACTAGTTACGTTTCTGTAATTTCTACTCTCTTATTTGTTCAGTTGGTCGAAATTTCTGTGATTTTTCTTTTTTTTTTACAAATATTTGATTGACCGTTGATTTTATTTTGGGAGGCGATGAAATTACTAGTTAGTTTCCTAATTTTCAGATGATTGGACTGTTGATTGAACTTATCTATATCATCAGTTGTTTATAATTTCGAAATAATGTTTTGATGACTGATTTTGATTGTTTTTACCTAGTTTTGTTGAACTTTTAGGCGAGGCCATGTCAATTATTGGCTATAATCGAATTTTGAGAAACTTGGAGCAATTAATTTACTCTGTATGATACACTTGATGTTTTAATTTAGAATTGAGCTCATTTCAATCACTGATTTATAATTTGTGAAATTAGTGATTATTAAGTTCATTTCTGTGTGACTTGGGTAGAAAGGTAGATATGGTGGATTTTTCATGAGTTTTTATGAAAATGAAAAAATTCATTTAGTTTGATGCATGAATGACTGACATTTTTTTTCTTCTTATTATTATTGCATCAGGTGATATAGTAGAGACTTTGAAGAAGAATGGATACGAGTATACTTGGGGCAATGTCACGGTGAAGCTTGCAGAATCCTTTGGATTTTGCTGGGGTGTGGAACGAGCCGTTCAGATTGCATATGAAGCGAGGAAACAGTTTCCCAGTGAGAGAATCTGGATCACTAACGAAATAATTCACAACCCAACTGTTAATAAGGTTTGCAAATTTCTTTCTGATTGATTTCTGTAGGATAATACAGTGGTAGTAGACTAGTAGCATCTTAAGATTTTGGAATGGAATTTGTGATTGATTAGGATCAGGTAGAATATCACTCCACCTCGAAAGTTATCATATGCCGAAAACCTGAGAACCGGGTAAAGTGAGAAATGATGTTGAATTTGCAAGCTGTTATCAAGATGGAGAAGCTTTTACAGAAAGCCAAACCTGTTTTCATGTCAAACCACGAATTCGTTTCTATTACCTTTGGGGTTAATTGATGTGGCTTTAAGTGTAGTTGCTTGACTTATATTTTTTCAGATCAGTTGATGGGATTAAGGTGTGGAGTTGGAAGATTAACAGTTAGTACCTAGAGGGAGTCTGTTAGTTCTTTTTAAATGTTGAGAGCTGTTCATATAATAAGTTAACTGGTACTTCGTATATCTCTTTTACGTGGGTTGGGCATCAGCTAAGCTTGTTAAATTTTGAGGTGTGAAACTTGCAACTTGAGGTTCAAAACCCATCTGCAAAAATTGACCTTGTGGCTCCCTATAACCTGCTTCCCTCAATCCCCAGTCCCCCACCCAACCATCCTCCCACCAAAAAAGAGAGTACTGCTTTTCTTTTCATTTATTTTGTTCTCTTTGTTAGACAATTAAAAGTGCCTCAAAATGAAAATGAATTACTACGGAGTACATTAAATAATTAGTCAGTCAGTTTTCTTCTATTGATCGCACGGCCTGCATTAAATAATTGACCACACCGCCTACATTAAATGATAGGATAGTAGCCCTTTTCTGTGCATGCTACGTTTACCTGAACAGAAAGCAGTCATCAGTAATTTGAAGTAACAGAAGTCTTTTGTTGCAGCGACTGGAAGAAATGGAAGTGCAGAATATCCCTCTTGAGGAAGGAAAGAAGCAATTTGAGGTTGTTGACAAAGGTGATGTTGTGATTTTACCAGCTTTTGGAGCTGCTGTAGATGAAATGTTGACTTTGAGCGAGAAAAATGTACAAATTGTAGATACAACTTGCCCTTGGGTGAGCAAGGTATGAATTCATAACCGATCTGCTGTTCCACTTGCTTCCCTCAGTGAGTATATGTTTGATAACCTTCACTGTCTCTGATTCTTTTCTATGTTCACTAATATTTTTTTTAGGTGTGGAACGTAGTGGAGAAGCACAAGAAAGGAGATTATACTTCAATTATTCATGGGAAATACGCTCATGAAGAGACCGTTGCTACTTCATCTTTTGCTGGGACATACATAATTGTGAAGAATATGAAAGAGGTAGATTACATGTTAATTTATTCCATATACTTCAATTTTCCATCACAGTAATACTGTACATTGCTTCATATCTTCTATGCTTGTGCTTCATCCAGGCAACTTATGTGTGTGATTATATTCTTGGTGGGCAACTTGATGGATCTAGTTCAACCAGAGAGGCGTTTCTAGAGGTAAATCTTCTTCTACTGAACATGATCGTAAATATCTTGGGCTTAAGGGACATCCTTCTCGTCTCTTTTACTCCCTTACTAATGGTATTTTCTTGCAGAAATTCAAATATGCTGTTTCCGATGGGTTTGATCCAGACAGAGACCTAGTAAAAACTGGAATAGCAAACCAAACTACAATGCTTAAGGGAGAAACAGAAGATATCGGTTTGTTGCACTTAGCAGAATAATTTTACTTGTTTTCTTGCAAGACTAGCAATTTAATGACTTCCTTGTCTCCAACTTTGCAGGAAAGCTTGTTGAGAGAACCATGATGCGGAAGTATGGGGTTGAGAACTTAAACAACCACTTCTTGAGTTTCAACACAATTTGCGATGCTACTCAAGTACTTCCACATCTCTTGCAACCTCTGTTCTTTTTTGCTTAAATGCTAGATAAGTAATTTCAGTAGCAATTTTTTTACTATTCTGCTTAATTTCTACCAGCTACCCAAATTAATGTTAGCCCATGGAAATCCTGCTCGATGCAGGGTTCATAGGCTGTAAACCCCAGATCGACGAAGTAATTTAGTACTTGGCTAAAAGTTGGTGATGGAAACAGAGGCCTTTTTGTAATTAGTCAAGTTCCTTCTGTTACAACTTTAGCATCCAAACAAGCATCTTTTCAGCCCCATTATTATAATATAGTGTCACCACGCAGTACATTGCCGTGTGCTACCTAAGTTGATGAAACATTCTTATGAAACTCTGGTGTGCTTACTTACTCTTTCTTCTCCAGGAGCGACAAGATGCAATGTATAAGCTGGTGGAAGAAAAAGTTGATCTTATGCTGGTGGTCGGAGGCTGGAACTCCAGCAACACCTCTCATTTGCAAGAAATAGTCGAGGAGCGTGGGATCCCTTCATATTGGATTGACAGTGAAGAAAGAATAGGCCCCGGAAACAAAATAAGCTACAAGTTGAACGTAAGTATAGAATACAGAAATCTTACTGCTATGTTATATTATCTCAATCTCAATGTTGTTACTCACCATATCATCTGTTCTCAGCATGGGGAGCTAGTTGAGAAAGAAAATTGGCTACCAGAAGGTCCAATCACTATCGGAATAACTTCTGGTGCTTCTACCCCAGATAAGGTATGCACCTAAAACGTTATTTTGGTTTGTGACAGCCCAAACCTTTACTCTGATCTCTTTGCTTTAATACTGCTAGCCTGCTAGCTAGAAACTCATGTGCAATGATTATGATATTATGCAGCACGTATAAGTGTACCACTGTTAACCTGCTAATAGAGATAAACGGGCTTCTGCGCCTCGTTGTTTAATGTTCAGTCCTTCAGGGTCATGTTAATGACAGATTAAAGTTGTTTTTATACCTAATTTTCAGTAATTCAGTGATTGACCCTAGTTATTTCTTTTTCTAGGTTGTGGAAGATGTCTTGGTCAAAGTTTTTGA

mRNA sequence

GTCGGGTTGGGTTGGGAAAATCTCAACCCGTTTATAAATGGGTCGACCTGATTTCGACCCAACCTAACCCATTGCCAGCCTCTAGGATTGATGGTGTAAGCGGATCGTGGGTAACCCGACTCATTGGCCATCGACCGATAAAGACGCTAGAATCCACAATTGGCAACCCATGTGTTTGGTGAGAAATGATTTTCTAATCACACAACAAATTACAACTTATTTATAAACGGACGACCAAATTAAAATACTCAATATTATTAACACACGGATAAAATTCCCGAAAATCCAAAACTTCATTCATACCCACTGAACTCCTGTAACCTTACAACTCACGTGTCTCTCTCTCCTCTTAGCTTCAATCAGTATCAAAAATGGCGATTTCTCCTCATCTCAGCCGCATCATCACCTTCACCGAACTCCGGTTGCCGGAAACTCAATTCCGCCCAAACTTCCACAGGAAGCCTCACCTCTCCGTCCGATGTGTCGCCGGTGAGTCTCCGACGTCTTCATCATCCTCCGCCGTTGATTCCAAATTTGATGCTAAGAAGTTCCATCACAACTTGACGAGGAGTGAGAATTATAATCGCAAGGGTTTTGGACACAAAGAAGAGACTCTCCAGCTTATGAATAGCGAGTATACTAGTGATATAGTAGAGACTTTGAAGAAGAATGGATACGAGTATACTTGGGGCAATGTCACGGTGAAGCTTGCAGAATCCTTTGGATTTTGCTGGGGTGTGGAACGAGCCGTTCAGATTGCATATGAAGCGAGGAAACAGTTTCCCAGTGAGAGAATCTGGATCACTAACGAAATAATTCACAACCCAACTGTTAATAAGCGACTGGAAGAAATGGAAGTGCAGAATATCCCTCTTGAGGAAGGAAAGAAGCAATTTGAGGTTGTTGACAAAGGTGATGTTGTGATTTTACCAGCTTTTGGAGCTGCTGTAGATGAAATGTTGACTTTGAGCGAGAAAAATGTACAAATTGTAGATACAACTTGCCCTTGGGTGAGCAAGGTGTGGAACGTAGTGGAGAAGCACAAGAAAGGAGATTATACTTCAATTATTCATGGGAAATACGCTCATGAAGAGACCGTTGCTACTTCATCTTTTGCTGGGACATACATAATTGTGAAGAATATGAAAGAGGCAACTTATGTGTGTGATTATATTCTTGGTGGGCAACTTGATGGATCTAGTTCAACCAGAGAGGCGTTTCTAGAGAAATTCAAATATGCTGTTTCCGATGGGTTTGATCCAGACAGAGACCTAGTAAAAACTGGAATAGCAAACCAAACTACAATGCTTAAGGGAGAAACAGAAGATATCGGAAAGCTTGTTGAGAGAACCATGATGCGGAAGTATGGGGTTGAGAACTTAAACAACCACTTCTTGAGTTTCAACACAATTTGCGATGCTACTCAAGAGCGACAAGATGCAATGTATAAGCTGGTGGAAGAAAAAGTTGATCTTATGCTGGTGGTCGGAGGCTGGAACTCCAGCAACACCTCTCATTTGCAAGAAATAGTCGAGGAGCGTGGGATCCCTTCATATTGGATTGACAGTGAAGAAAGAATAGGCCCCGGAAACAAAATAAGCTACAAGTTGAACCATGGGGAGCTAGTTGAGAAAGAAAATTGGCTACCAGAAGGTCCAATCACTATCGGAATAACTTCTGGTGCTTCTACCCCAGATAAGTTATTTCTTTTTCTAGGTTGTGGAAGATGTCTTGGTCAAAGTTTTTGA

Coding sequence (CDS)

ATGGCGATTTCTCCTCATCTCAGCCGCATCATCACCTTCACCGAACTCCGGTTGCCGGAAACTCAATTCCGCCCAAACTTCCACAGGAAGCCTCACCTCTCCGTCCGATGTGTCGCCGGTGAGTCTCCGACGTCTTCATCATCCTCCGCCGTTGATTCCAAATTTGATGCTAAGAAGTTCCATCACAACTTGACGAGGAGTGAGAATTATAATCGCAAGGGTTTTGGACACAAAGAAGAGACTCTCCAGCTTATGAATAGCGAGTATACTAGTGATATAGTAGAGACTTTGAAGAAGAATGGATACGAGTATACTTGGGGCAATGTCACGGTGAAGCTTGCAGAATCCTTTGGATTTTGCTGGGGTGTGGAACGAGCCGTTCAGATTGCATATGAAGCGAGGAAACAGTTTCCCAGTGAGAGAATCTGGATCACTAACGAAATAATTCACAACCCAACTGTTAATAAGCGACTGGAAGAAATGGAAGTGCAGAATATCCCTCTTGAGGAAGGAAAGAAGCAATTTGAGGTTGTTGACAAAGGTGATGTTGTGATTTTACCAGCTTTTGGAGCTGCTGTAGATGAAATGTTGACTTTGAGCGAGAAAAATGTACAAATTGTAGATACAACTTGCCCTTGGGTGAGCAAGGTGTGGAACGTAGTGGAGAAGCACAAGAAAGGAGATTATACTTCAATTATTCATGGGAAATACGCTCATGAAGAGACCGTTGCTACTTCATCTTTTGCTGGGACATACATAATTGTGAAGAATATGAAAGAGGCAACTTATGTGTGTGATTATATTCTTGGTGGGCAACTTGATGGATCTAGTTCAACCAGAGAGGCGTTTCTAGAGAAATTCAAATATGCTGTTTCCGATGGGTTTGATCCAGACAGAGACCTAGTAAAAACTGGAATAGCAAACCAAACTACAATGCTTAAGGGAGAAACAGAAGATATCGGAAAGCTTGTTGAGAGAACCATGATGCGGAAGTATGGGGTTGAGAACTTAAACAACCACTTCTTGAGTTTCAACACAATTTGCGATGCTACTCAAGAGCGACAAGATGCAATGTATAAGCTGGTGGAAGAAAAAGTTGATCTTATGCTGGTGGTCGGAGGCTGGAACTCCAGCAACACCTCTCATTTGCAAGAAATAGTCGAGGAGCGTGGGATCCCTTCATATTGGATTGACAGTGAAGAAAGAATAGGCCCCGGAAACAAAATAAGCTACAAGTTGAACCATGGGGAGCTAGTTGAGAAAGAAAATTGGCTACCAGAAGGTCCAATCACTATCGGAATAACTTCTGGTGCTTCTACCCCAGATAAGTTATTTCTTTTTCTAGGTTGTGGAAGATGTCTTGGTCAAAGTTTTTGA

Protein sequence

MAISPHLSRIITFTELRLPETQFRPNFHRKPHLSVRCVAGESPTSSSSSAVDSKFDAKKFHHNLTRSENYNRKGFGHKEETLQLMNSEYTSDIVETLKKNGYEYTWGNVTVKLAESFGFCWGVERAVQIAYEARKQFPSERIWITNEIIHNPTVNKRLEEMEVQNIPLEEGKKQFEVVDKGDVVILPAFGAAVDEMLTLSEKNVQIVDTTCPWVSKVWNVVEKHKKGDYTSIIHGKYAHEETVATSSFAGTYIIVKNMKEATYVCDYILGGQLDGSSSTREAFLEKFKYAVSDGFDPDRDLVKTGIANQTTMLKGETEDIGKLVERTMMRKYGVENLNNHFLSFNTICDATQERQDAMYKLVEEKVDLMLVVGGWNSSNTSHLQEIVEERGIPSYWIDSEERIGPGNKISYKLNHGELVEKENWLPEGPITIGITSGASTPDKLFLFLGCGRCLGQSF

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo03335	Spo03335	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo03335.1	Spo03335.1-protein	polypeptide

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo03335.1.exon.1	Spo03335.1.exon.1	exon
Spo03335.1.exon.2	Spo03335.1.exon.2	exon
Spo03335.1.exon.3	Spo03335.1.exon.3	exon
Spo03335.1.exon.4	Spo03335.1.exon.4	exon
Spo03335.1.exon.5	Spo03335.1.exon.5	exon
Spo03335.1.exon.6	Spo03335.1.exon.6	exon
Spo03335.1.exon.7	Spo03335.1.exon.7	exon
Spo03335.1.exon.8	Spo03335.1.exon.8	exon
Spo03335.1.exon.9	Spo03335.1.exon.9	exon
Spo03335.1.exon.10	Spo03335.1.exon.10	exon

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo03335.1.utr5p.1	Spo03335.1.utr5p.1	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo03335.1.CDS.1	Spo03335.1.CDS.1	CDS
Spo03335.1.CDS.2	Spo03335.1.CDS.2	CDS
Spo03335.1.CDS.3	Spo03335.1.CDS.3	CDS
Spo03335.1.CDS.4	Spo03335.1.CDS.4	CDS
Spo03335.1.CDS.5	Spo03335.1.CDS.5	CDS
Spo03335.1.CDS.6	Spo03335.1.CDS.6	CDS
Spo03335.1.CDS.7	Spo03335.1.CDS.7	CDS
Spo03335.1.CDS.8	Spo03335.1.CDS.8	CDS
Spo03335.1.CDS.9	Spo03335.1.CDS.9	CDS
Spo03335.1.CDS.10	Spo03335.1.CDS.10	CDS

Homology

[Close]

BLAST of Spo03335.1 vs. NCBI nr
Match: gi|902186035|gb|KNA10609.1| (hypothetical protein SOVF_142790 [Spinacia oleracea])

HSP 1 Score: 903.3 bits (2333), Expect = 1.800e-259
Identity = 443/444 (99.77%), Postives = 444/444 (100.00%), Query Frame = 1


		  
Query: 1   MAISPHLSRIITFTELRLPETQFRPNFHRKPHLSVRCVAGESPTSSSSSAVDSKFDAKKF 60
           MAISPHLSRIITFTELRLPETQFRPNFHRKPHLSVRCVAGESPTSSSSSAVDSKFDAKKF
Sbjct: 1   MAISPHLSRIITFTELRLPETQFRPNFHRKPHLSVRCVAGESPTSSSSSAVDSKFDAKKF 60

Query: 61  HHNLTRSENYNRKGFGHKEETLQLMNSEYTSDIVETLKKNGYEYTWGNVTVKLAESFGFC 120
           HHNLTRSENYNRKGFGHKEETLQLMNSEYTSDIVETLKKNGYEYTWGNVTVKLAESFGFC
Sbjct: 61  HHNLTRSENYNRKGFGHKEETLQLMNSEYTSDIVETLKKNGYEYTWGNVTVKLAESFGFC 120

Query: 121 WGVERAVQIAYEARKQFPSERIWITNEIIHNPTVNKRLEEMEVQNIPLEEGKKQFEVVDK 180
           WGVERAVQIAYEARKQFPSERIWITNEIIHNPTVNKRLEEMEVQNIPLEEGKKQFEVVDK
Sbjct: 121 WGVERAVQIAYEARKQFPSERIWITNEIIHNPTVNKRLEEMEVQNIPLEEGKKQFEVVDK 180

Query: 181 GDVVILPAFGAAVDEMLTLSEKNVQIVDTTCPWVSKVWNVVEKHKKGDYTSIIHGKYAHE 240
           GDVVILPAFGAAVDEMLTLSEKNVQIVDTTCPWVSKVWNVVEKHKKGDYTSIIHGKYAHE
Sbjct: 181 GDVVILPAFGAAVDEMLTLSEKNVQIVDTTCPWVSKVWNVVEKHKKGDYTSIIHGKYAHE 240

Query: 241 ETVATSSFAGTYIIVKNMKEATYVCDYILGGQLDGSSSTREAFLEKFKYAVSDGFDPDRD 300
           ETVATSSFAGTYIIVKNMKEATYVCDYILGGQLDGSSSTREAFLEKFKYAVSDGFDPDRD
Sbjct: 241 ETVATSSFAGTYIIVKNMKEATYVCDYILGGQLDGSSSTREAFLEKFKYAVSDGFDPDRD 300

Query: 301 LVKTGIANQTTMLKGETEDIGKLVERTMMRKYGVENLNNHFLSFNTICDATQERQDAMYK 360
           LVKTGIANQTTMLKGETEDIGKLVERTMMRKYGVENLNNHFLSFNTICDATQERQDAMYK
Sbjct: 301 LVKTGIANQTTMLKGETEDIGKLVERTMMRKYGVENLNNHFLSFNTICDATQERQDAMYK 360

Query: 361 LVEEKVDLMLVVGGWNSSNTSHLQEIVEERGIPSYWIDSEERIGPGNKISYKLNHGELVE 420
           LVEEKVDLMLVVGGWNSSNTSHLQEIVEERGIPSYWIDSEERIGPGNKISYKLNHGELVE
Sbjct: 361 LVEEKVDLMLVVGGWNSSNTSHLQEIVEERGIPSYWIDSEERIGPGNKISYKLNHGELVE 420

Query: 421 KENWLPEGPITIGITSGASTPDKL 445
           KENWLPEGPITIGITSGASTPDK+
Sbjct: 421 KENWLPEGPITIGITSGASTPDKV 444

[Close]

BLAST of Spo03335.1 vs. NCBI nr
Match: gi|731358793|ref|XP_010690939.1| (PREDICTED: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 803.1 bits (2073), Expect = 2.500e-229
Identity = 391/444 (88.06%), Postives = 416/444 (93.69%), Query Frame = 1


		  
Query: 1   MAISPHLSRIITFTELRLPETQFRPNFHRKPHLSVRCVAGESPTSSSSSAVDSKFDAKKF 60
           MAISPHLS I T T+  LPE+  R  F+RK HL+VRC  G+S   SSSSA D+ FDAKKF
Sbjct: 1   MAISPHLSHIFTSTKHHLPESYTRSIFYRKTHLTVRCSGGDS--LSSSSAGDTNFDAKKF 60

Query: 61  HHNLTRSENYNRKGFGHKEETLQLMNSEYTSDIVETLKKNGYEYTWGNVTVKLAESFGFC 120
           HHNLTRS+NYNRKGFG KEETLQLMN EYTSDIVETLK NG+EYTWGNVTVKLAE+FGFC
Sbjct: 61  HHNLTRSDNYNRKGFGRKEETLQLMNREYTSDIVETLKDNGFEYTWGNVTVKLAEAFGFC 120

Query: 121 WGVERAVQIAYEARKQFPSERIWITNEIIHNPTVNKRLEEMEVQNIPLEEGKKQFEVVDK 180
           WGVERAVQIAYEAR QFPSERIWITNEIIHNPTVNKRLEEMEVQNIPLE+GKKQFEVVDK
Sbjct: 121 WGVERAVQIAYEARTQFPSERIWITNEIIHNPTVNKRLEEMEVQNIPLEDGKKQFEVVDK 180

Query: 181 GDVVILPAFGAAVDEMLTLSEKNVQIVDTTCPWVSKVWNVVEKHKKGDYTSIIHGKYAHE 240
           GDVVILPAFGAAV+EMLTLSEK VQIVDTTCPWV+KVWNVVEKHKKGDYTSIIHGKYAHE
Sbjct: 181 GDVVILPAFGAAVEEMLTLSEKKVQIVDTTCPWVNKVWNVVEKHKKGDYTSIIHGKYAHE 240

Query: 241 ETVATSSFAGTYIIVKNMKEATYVCDYILGGQLDGSSSTREAFLEKFKYAVSDGFDPDRD 300
           ET+ATSSFA TYIIVKNMKEATYVCDYILGG+LDGSSSTREAFLEKFKYAVS GFDPD D
Sbjct: 241 ETIATSSFAQTYIIVKNMKEATYVCDYILGGKLDGSSSTREAFLEKFKYAVSSGFDPDTD 300

Query: 301 LVKTGIANQTTMLKGETEDIGKLVERTMMRKYGVENLNNHFLSFNTICDATQERQDAMYK 360
           L+K GIANQTTMLKGETE+IGKLVERTMMRKYGVEN+NNHF+SFNTICDATQERQDAMYK
Sbjct: 301 LIKVGIANQTTMLKGETEEIGKLVERTMMRKYGVENVNNHFMSFNTICDATQERQDAMYK 360

Query: 361 LVEEKVDLMLVVGGWNSSNTSHLQEIVEERGIPSYWIDSEERIGPGNKISYKLNHGELVE 420
           LV+EK+DL+LV+GGWNSSNTSHLQEI EERGIPSYWIDSE+RIGPGNKISYKL HGELVE
Sbjct: 361 LVDEKIDLILVIGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNKISYKLMHGELVE 420

Query: 421 KENWLPEGPITIGITSGASTPDKL 445
           KENWLPEGPITIG+TSGASTPDK+
Sbjct: 421 KENWLPEGPITIGVTSGASTPDKV 442

[Close]

BLAST of Spo03335.1 vs. NCBI nr
Match: gi|747082064|ref|XP_011088335.1| (PREDICTED: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic [Sesamum indicum])

HSP 1 Score: 754.6 bits (1947), Expect = 1.000e-214
Identity = 364/445 (81.80%), Postives = 406/445 (91.24%), Query Frame = 1


		  
Query: 1   MAISPHLSRIITFTELRLPETQFRPNFHRKPHLSVRC-VAGESPTSSSSSAVDSKFDAKK 60
           MAIS H SR+ T   L +PET+      R+   S+RC  AG++ + SS+SA  S FDAK 
Sbjct: 1   MAISMHFSRVFTRPHLSVPETRLS---RRRTPPSLRCSAAGDAASLSSASAESSDFDAKV 60

Query: 61  FHHNLTRSENYNRKGFGHKEETLQLMNSEYTSDIVETLKKNGYEYTWGNVTVKLAESFGF 120
           F HNLTRS+NYNRKGFGHKEETL+ M  EYTSDI++TLK+NGYEY WGNVTVKLAE++GF
Sbjct: 61  FRHNLTRSKNYNRKGFGHKEETLEQMTEEYTSDIIKTLKENGYEYRWGNVTVKLAEAYGF 120

Query: 121 CWGVERAVQIAYEARKQFPSERIWITNEIIHNPTVNKRLEEMEVQNIPLEEGKKQFEVVD 180
           CWGVERAVQIAYEARKQFP+E+IW+TNEIIHNPTVNKRLE+MEV+NIP++ GKKQF+VV+
Sbjct: 121 CWGVERAVQIAYEARKQFPAEKIWLTNEIIHNPTVNKRLEDMEVKNIPIDNGKKQFDVVE 180

Query: 181 KGDVVILPAFGAAVDEMLTLSEKNVQIVDTTCPWVSKVWNVVEKHKKGDYTSIIHGKYAH 240
           K DVV+LPAFGAAVDEML LSEKNVQIVDTTCPWVSKVWN VEKHKKG+YTSIIHGKY+H
Sbjct: 181 KADVVVLPAFGAAVDEMLVLSEKNVQIVDTTCPWVSKVWNTVEKHKKGEYTSIIHGKYSH 240

Query: 241 EETVATSSFAGTYIIVKNMKEATYVCDYILGGQLDGSSSTREAFLEKFKYAVSDGFDPDR 300
           EETVAT+SFAG YIIVKNMKEATYVCDYILGG LDGSSST+EAFLEKFK+AVS GFDPD+
Sbjct: 241 EETVATASFAGKYIIVKNMKEATYVCDYILGGGLDGSSSTKEAFLEKFKFAVSKGFDPDK 300

Query: 301 DLVKTGIANQTTMLKGETEDIGKLVERTMMRKYGVENLNNHFLSFNTICDATQERQDAMY 360
           DLVK GIANQTTMLKGETEDIGKLVERT+MRKYGVEN+NNHF+SFNTICDATQERQDAMY
Sbjct: 301 DLVKVGIANQTTMLKGETEDIGKLVERTIMRKYGVENINNHFISFNTICDATQERQDAMY 360

Query: 361 KLVEEKVDLMLVVGGWNSSNTSHLQEIVEERGIPSYWIDSEERIGPGNKISYKLNHGELV 420
           KLV++++DLMLVVGGWNSSNTSHLQEI EERGIPSYWIDSE+RIGPGNKISYKL HGELV
Sbjct: 361 KLVDQQLDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEKRIGPGNKISYKLMHGELV 420

Query: 421 EKENWLPEGPITIGITSGASTPDKL 445
           EKENWLPEGP+TIGITSGASTPDK+
Sbjct: 421 EKENWLPEGPVTIGITSGASTPDKV 442

[Close]

BLAST of Spo03335.1 vs. NCBI nr
Match: gi|703097851|ref|XP_010096225.1| (4-hydroxy-3-methylbut-2-enyl diphosphate reductase [Morus notabilis])

HSP 1 Score: 754.2 bits (1946), Expect = 1.300e-214
Identity = 372/444 (83.78%), Postives = 405/444 (91.22%), Query Frame = 1


		  
Query: 1   MAISPHLSRIITFTELRLPET-QFRPNFHRKPHLSVRCVAGESPTSSSSSAVDSKFDAKK 60
           MAI+  L RI T T+L LPET   R    RKP LSVRC    S  SS   AV+S FDAK 
Sbjct: 37  MAIAFQLCRIPTRTDLFLPETFSLRC---RKP-LSVRCSGDSSSASSPFVAVESDFDAKV 96

Query: 61  FHHNLTRSENYNRKGFGHKEETLQLMNSEYTSDIVETLKKNGYEYTWGNVTVKLAESFGF 120
           F  NLTRS+NYNRKGFGHKEETL+LMN EYTSDI++TLK+NG EYTWGNVTVKLAES+GF
Sbjct: 97  FRKNLTRSKNYNRKGFGHKEETLELMNREYTSDIIKTLKENGNEYTWGNVTVKLAESYGF 156

Query: 121 CWGVERAVQIAYEARKQFPSERIWITNEIIHNPTVNKRLEEMEVQNIPLEEGKKQFEVVD 180
           CWGVERAVQIAYEARKQFP E+IWITNEIIHNPTVNKRLEEM+V+NIP+EEG KQFEVVD
Sbjct: 157 CWGVERAVQIAYEARKQFPDEKIWITNEIIHNPTVNKRLEEMKVENIPIEEGNKQFEVVD 216

Query: 181 KGDVVILPAFGAAVDEMLTLSEKNVQIVDTTCPWVSKVWNVVEKHKKGDYTSIIHGKYAH 240
           KGDVVILPAFGAAVDEMLTLS+KNVQIVDTTCPWVSKVWN VEKHKKGDYTSIIHGKY+H
Sbjct: 217 KGDVVILPAFGAAVDEMLTLSDKNVQIVDTTCPWVSKVWNTVEKHKKGDYTSIIHGKYSH 276

Query: 241 EETVATSSFAGTYIIVKNMKEATYVCDYILGGQLDGSSSTREAFLEKFKYAVSDGFDPDR 300
           EET+AT+SFAGTYIIVKNM+EATYVCDYILGG LDGSSSTR AFLEKFKYAVSDGFDPD+
Sbjct: 277 EETIATASFAGTYIIVKNMQEATYVCDYILGGALDGSSSTRAAFLEKFKYAVSDGFDPDK 336

Query: 301 DLVKTGIANQTTMLKGETEDIGKLVERTMMRKYGVENLNNHFLSFNTICDATQERQDAMY 360
           DLVK GIANQTTMLKGETE+IGKL ERT+M+KYGVEN+N+HF+SFNTICDATQERQDAMY
Sbjct: 337 DLVKVGIANQTTMLKGETEEIGKLAERTLMQKYGVENVNDHFVSFNTICDATQERQDAMY 396

Query: 361 KLVEEKVDLMLVVGGWNSSNTSHLQEIVEERGIPSYWIDSEERIGPGNKISYKLNHGELV 420
           KLVE+ +DL+LVVGGWNSSNTSHLQEI EERGIPSYWIDSE+RIGPGN+I+YKLNHGELV
Sbjct: 397 KLVEDDLDLILVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHGELV 456

Query: 421 EKENWLPEGPITIGITSGASTPDK 444
           EKENWLP+GP+ IGITSGASTPDK
Sbjct: 457 EKENWLPKGPLMIGITSGASTPDK 476

[Close]

BLAST of Spo03335.1 vs. NCBI nr
Match: gi|747090179|ref|XP_011092767.1| (PREDICTED: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic-like [Sesamum indicum])

HSP 1 Score: 753.8 bits (1945), Expect = 1.700e-214
Identity = 372/446 (83.41%), Postives = 411/446 (92.15%), Query Frame = 1


		  
Query: 1   MAISPHLSRIITFTELRLPETQFRPNFHRKPHLSVRC-VAGESPTSSSSSAVDSK-FDAK 60
           MAIS H SRI T TEL  PET+    F ++   SVRC  AGE+ TSSS   V+S+ FDAK
Sbjct: 1   MAISLHFSRISTRTELLRPETRI---FRQRKPSSVRCSAAGEAATSSS---VESEGFDAK 60

Query: 61  KFHHNLTRSENYNRKGFGHKEETLQLMNSEYTSDIVETLKKNGYEYTWGNVTVKLAESFG 120
            F HNLTRS+NYNRKGFG+KEETL+ M+ EYTSDI++TLK+NGYEYTWGNVTVKLAE++G
Sbjct: 61  VFRHNLTRSKNYNRKGFGYKEETLEQMSQEYTSDIIKTLKENGYEYTWGNVTVKLAEAYG 120

Query: 121 FCWGVERAVQIAYEARKQFPSERIWITNEIIHNPTVNKRLEEMEVQNIPLEEGKKQFEVV 180
           FCWGVERAVQIAYEARKQFP+E+IWITNEIIHNPTVN+RLEEMEV+NIPL++GKKQF+VV
Sbjct: 121 FCWGVERAVQIAYEARKQFPTEKIWITNEIIHNPTVNQRLEEMEVKNIPLDDGKKQFDVV 180

Query: 181 DKGDVVILPAFGAAVDEMLTLSEKNVQIVDTTCPWVSKVWNVVEKHKKGDYTSIIHGKYA 240
           DKGDVV+LPAFGAAVDEML LSEKNVQIVDTTCPWVSKVWNVVEKHKKG+YTSIIHGKY+
Sbjct: 181 DKGDVVVLPAFGAAVDEMLVLSEKNVQIVDTTCPWVSKVWNVVEKHKKGEYTSIIHGKYS 240

Query: 241 HEETVATSSFAGTYIIVKNMKEATYVCDYILGGQLDGSSSTREAFLEKFKYAVSDGFDPD 300
           HEETVAT+SFAG YIIVKNM EA YVCDYILGG LDGSSST+EAF+EKFK AVS+GFDPD
Sbjct: 241 HEETVATASFAGKYIIVKNMTEANYVCDYILGGGLDGSSSTKEAFMEKFKLAVSEGFDPD 300

Query: 301 RDLVKTGIANQTTMLKGETEDIGKLVERTMMRKYGVENLNNHFLSFNTICDATQERQDAM 360
            DLVK GIANQTTMLKGETE+IG+LVERTMMRKYGVEN+N+HFLSFNTICDATQERQDAM
Sbjct: 301 VDLVKVGIANQTTMLKGETEEIGRLVERTMMRKYGVENVNSHFLSFNTICDATQERQDAM 360

Query: 361 YKLVEEKVDLMLVVGGWNSSNTSHLQEIVEERGIPSYWIDSEERIGPGNKISYKLNHGEL 420
           YKLV+++VDLMLVVGGWNSSNTSHLQEI EERGIPSYWIDSE+RIGPGNKISYKL HGEL
Sbjct: 361 YKLVDQQVDLMLVVGGWNSSNTSHLQEIAEERGIPSYWIDSEKRIGPGNKISYKLLHGEL 420

Query: 421 VEKENWLPEGPITIGITSGASTPDKL 445
           VEKENWLPEGPIT+GITSGASTPDK+
Sbjct: 421 VEKENWLPEGPITVGITSGASTPDKV 440

[Close]

BLAST of Spo03335.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QTN2_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_142790 PE=3 SV=1)

HSP 1 Score: 903.3 bits (2333), Expect = 1.200e-259
Identity = 443/444 (99.77%), Postives = 444/444 (100.00%), Query Frame = 1


		  
Query: 1   MAISPHLSRIITFTELRLPETQFRPNFHRKPHLSVRCVAGESPTSSSSSAVDSKFDAKKF 60
           MAISPHLSRIITFTELRLPETQFRPNFHRKPHLSVRCVAGESPTSSSSSAVDSKFDAKKF
Sbjct: 1   MAISPHLSRIITFTELRLPETQFRPNFHRKPHLSVRCVAGESPTSSSSSAVDSKFDAKKF 60

Query: 61  HHNLTRSENYNRKGFGHKEETLQLMNSEYTSDIVETLKKNGYEYTWGNVTVKLAESFGFC 120
           HHNLTRSENYNRKGFGHKEETLQLMNSEYTSDIVETLKKNGYEYTWGNVTVKLAESFGFC
Sbjct: 61  HHNLTRSENYNRKGFGHKEETLQLMNSEYTSDIVETLKKNGYEYTWGNVTVKLAESFGFC 120

Query: 121 WGVERAVQIAYEARKQFPSERIWITNEIIHNPTVNKRLEEMEVQNIPLEEGKKQFEVVDK 180
           WGVERAVQIAYEARKQFPSERIWITNEIIHNPTVNKRLEEMEVQNIPLEEGKKQFEVVDK
Sbjct: 121 WGVERAVQIAYEARKQFPSERIWITNEIIHNPTVNKRLEEMEVQNIPLEEGKKQFEVVDK 180

Query: 181 GDVVILPAFGAAVDEMLTLSEKNVQIVDTTCPWVSKVWNVVEKHKKGDYTSIIHGKYAHE 240
           GDVVILPAFGAAVDEMLTLSEKNVQIVDTTCPWVSKVWNVVEKHKKGDYTSIIHGKYAHE
Sbjct: 181 GDVVILPAFGAAVDEMLTLSEKNVQIVDTTCPWVSKVWNVVEKHKKGDYTSIIHGKYAHE 240

Query: 241 ETVATSSFAGTYIIVKNMKEATYVCDYILGGQLDGSSSTREAFLEKFKYAVSDGFDPDRD 300
           ETVATSSFAGTYIIVKNMKEATYVCDYILGGQLDGSSSTREAFLEKFKYAVSDGFDPDRD
Sbjct: 241 ETVATSSFAGTYIIVKNMKEATYVCDYILGGQLDGSSSTREAFLEKFKYAVSDGFDPDRD 300

Query: 301 LVKTGIANQTTMLKGETEDIGKLVERTMMRKYGVENLNNHFLSFNTICDATQERQDAMYK 360
           LVKTGIANQTTMLKGETEDIGKLVERTMMRKYGVENLNNHFLSFNTICDATQERQDAMYK
Sbjct: 301 LVKTGIANQTTMLKGETEDIGKLVERTMMRKYGVENLNNHFLSFNTICDATQERQDAMYK 360

Query: 361 LVEEKVDLMLVVGGWNSSNTSHLQEIVEERGIPSYWIDSEERIGPGNKISYKLNHGELVE 420
           LVEEKVDLMLVVGGWNSSNTSHLQEIVEERGIPSYWIDSEERIGPGNKISYKLNHGELVE
Sbjct: 361 LVEEKVDLMLVVGGWNSSNTSHLQEIVEERGIPSYWIDSEERIGPGNKISYKLNHGELVE 420

Query: 421 KENWLPEGPITIGITSGASTPDKL 445
           KENWLPEGPITIGITSGASTPDK+
Sbjct: 421 KENWLPEGPITIGITSGASTPDKV 444

[Close]

BLAST of Spo03335.1 vs. UniProtKB/TrEMBL
Match: A0A0J8BK59_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_9g217920 PE=3 SV=1)

HSP 1 Score: 803.1 bits (2073), Expect = 1.700e-229
Identity = 391/444 (88.06%), Postives = 416/444 (93.69%), Query Frame = 1


		  
Query: 1   MAISPHLSRIITFTELRLPETQFRPNFHRKPHLSVRCVAGESPTSSSSSAVDSKFDAKKF 60
           MAISPHLS I T T+  LPE+  R  F+RK HL+VRC  G+S   SSSSA D+ FDAKKF
Sbjct: 1   MAISPHLSHIFTSTKHHLPESYTRSIFYRKTHLTVRCSGGDS--LSSSSAGDTNFDAKKF 60

Query: 61  HHNLTRSENYNRKGFGHKEETLQLMNSEYTSDIVETLKKNGYEYTWGNVTVKLAESFGFC 120
           HHNLTRS+NYNRKGFG KEETLQLMN EYTSDIVETLK NG+EYTWGNVTVKLAE+FGFC
Sbjct: 61  HHNLTRSDNYNRKGFGRKEETLQLMNREYTSDIVETLKDNGFEYTWGNVTVKLAEAFGFC 120

Query: 121 WGVERAVQIAYEARKQFPSERIWITNEIIHNPTVNKRLEEMEVQNIPLEEGKKQFEVVDK 180
           WGVERAVQIAYEAR QFPSERIWITNEIIHNPTVNKRLEEMEVQNIPLE+GKKQFEVVDK
Sbjct: 121 WGVERAVQIAYEARTQFPSERIWITNEIIHNPTVNKRLEEMEVQNIPLEDGKKQFEVVDK 180

Query: 181 GDVVILPAFGAAVDEMLTLSEKNVQIVDTTCPWVSKVWNVVEKHKKGDYTSIIHGKYAHE 240
           GDVVILPAFGAAV+EMLTLSEK VQIVDTTCPWV+KVWNVVEKHKKGDYTSIIHGKYAHE
Sbjct: 181 GDVVILPAFGAAVEEMLTLSEKKVQIVDTTCPWVNKVWNVVEKHKKGDYTSIIHGKYAHE 240

Query: 241 ETVATSSFAGTYIIVKNMKEATYVCDYILGGQLDGSSSTREAFLEKFKYAVSDGFDPDRD 300
           ET+ATSSFA TYIIVKNMKEATYVCDYILGG+LDGSSSTREAFLEKFKYAVS GFDPD D
Sbjct: 241 ETIATSSFAQTYIIVKNMKEATYVCDYILGGKLDGSSSTREAFLEKFKYAVSSGFDPDTD 300

Query: 301 LVKTGIANQTTMLKGETEDIGKLVERTMMRKYGVENLNNHFLSFNTICDATQERQDAMYK 360
           L+K GIANQTTMLKGETE+IGKLVERTMMRKYGVEN+NNHF+SFNTICDATQERQDAMYK
Sbjct: 301 LIKVGIANQTTMLKGETEEIGKLVERTMMRKYGVENVNNHFMSFNTICDATQERQDAMYK 360

Query: 361 LVEEKVDLMLVVGGWNSSNTSHLQEIVEERGIPSYWIDSEERIGPGNKISYKLNHGELVE 420
           LV+EK+DL+LV+GGWNSSNTSHLQEI EERGIPSYWIDSE+RIGPGNKISYKL HGELVE
Sbjct: 361 LVDEKIDLILVIGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNKISYKLMHGELVE 420

Query: 421 KENWLPEGPITIGITSGASTPDKL 445
           KENWLPEGPITIG+TSGASTPDK+
Sbjct: 421 KENWLPEGPITIGVTSGASTPDKV 442

[Close]

BLAST of Spo03335.1 vs. UniProtKB/TrEMBL
Match: W9R118_9ROSA (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Morus notabilis GN=L484_026961 PE=3 SV=1)

HSP 1 Score: 754.2 bits (1946), Expect = 9.200e-215
Identity = 372/444 (83.78%), Postives = 405/444 (91.22%), Query Frame = 1


		  
Query: 1   MAISPHLSRIITFTELRLPET-QFRPNFHRKPHLSVRCVAGESPTSSSSSAVDSKFDAKK 60
           MAI+  L RI T T+L LPET   R    RKP LSVRC    S  SS   AV+S FDAK 
Sbjct: 37  MAIAFQLCRIPTRTDLFLPETFSLRC---RKP-LSVRCSGDSSSASSPFVAVESDFDAKV 96

Query: 61  FHHNLTRSENYNRKGFGHKEETLQLMNSEYTSDIVETLKKNGYEYTWGNVTVKLAESFGF 120
           F  NLTRS+NYNRKGFGHKEETL+LMN EYTSDI++TLK+NG EYTWGNVTVKLAES+GF
Sbjct: 97  FRKNLTRSKNYNRKGFGHKEETLELMNREYTSDIIKTLKENGNEYTWGNVTVKLAESYGF 156

Query: 121 CWGVERAVQIAYEARKQFPSERIWITNEIIHNPTVNKRLEEMEVQNIPLEEGKKQFEVVD 180
           CWGVERAVQIAYEARKQFP E+IWITNEIIHNPTVNKRLEEM+V+NIP+EEG KQFEVVD
Sbjct: 157 CWGVERAVQIAYEARKQFPDEKIWITNEIIHNPTVNKRLEEMKVENIPIEEGNKQFEVVD 216

Query: 181 KGDVVILPAFGAAVDEMLTLSEKNVQIVDTTCPWVSKVWNVVEKHKKGDYTSIIHGKYAH 240
           KGDVVILPAFGAAVDEMLTLS+KNVQIVDTTCPWVSKVWN VEKHKKGDYTSIIHGKY+H
Sbjct: 217 KGDVVILPAFGAAVDEMLTLSDKNVQIVDTTCPWVSKVWNTVEKHKKGDYTSIIHGKYSH 276

Query: 241 EETVATSSFAGTYIIVKNMKEATYVCDYILGGQLDGSSSTREAFLEKFKYAVSDGFDPDR 300
           EET+AT+SFAGTYIIVKNM+EATYVCDYILGG LDGSSSTR AFLEKFKYAVSDGFDPD+
Sbjct: 277 EETIATASFAGTYIIVKNMQEATYVCDYILGGALDGSSSTRAAFLEKFKYAVSDGFDPDK 336

Query: 301 DLVKTGIANQTTMLKGETEDIGKLVERTMMRKYGVENLNNHFLSFNTICDATQERQDAMY 360
           DLVK GIANQTTMLKGETE+IGKL ERT+M+KYGVEN+N+HF+SFNTICDATQERQDAMY
Sbjct: 337 DLVKVGIANQTTMLKGETEEIGKLAERTLMQKYGVENVNDHFVSFNTICDATQERQDAMY 396

Query: 361 KLVEEKVDLMLVVGGWNSSNTSHLQEIVEERGIPSYWIDSEERIGPGNKISYKLNHGELV 420
           KLVE+ +DL+LVVGGWNSSNTSHLQEI EERGIPSYWIDSE+RIGPGN+I+YKLNHGELV
Sbjct: 397 KLVEDDLDLILVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHGELV 456

Query: 421 EKENWLPEGPITIGITSGASTPDK 444
           EKENWLP+GP+ IGITSGASTPDK
Sbjct: 457 EKENWLPKGPLMIGITSGASTPDK 476

[Close]

BLAST of Spo03335.1 vs. UniProtKB/TrEMBL
Match: A0A022RCG7_ERYGU (Uncharacterized protein OS=Erythranthe guttata GN=MIMGU_mgv1a005937mg PE=3 SV=1)

HSP 1 Score: 752.3 bits (1941), Expect = 3.500e-214
Identity = 368/453 (81.24%), Postives = 409/453 (90.29%), Query Frame = 1


		  
Query: 1   MAISPHLSRIITFTELRLPETQFRPNFHRKPHLSVRC-VAGESPTSSSSSAV--DSKFDA 60
           MAIS    RI    EL LPET+    F ++   SVRC  AGE+ +SSSSSA    S+FDA
Sbjct: 1   MAISLQFCRISARAELSLPETRI---FRQRKPSSVRCSAAGEAASSSSSSATTESSEFDA 60

Query: 61  KKFHHNLTRSENYNRKGFGHKEETLQLMNSEYTSDIVETLKKNGYEYTWGNVTVKLAESF 120
           K F HNLTRSENYNR+GFGHK+ETL+ M+ EYTSDI++TLK+NG+EYTWGNVTVKLAES+
Sbjct: 61  KVFRHNLTRSENYNRRGFGHKKETLERMSQEYTSDIIKTLKENGFEYTWGNVTVKLAESY 120

Query: 121 GFCWGVERAVQIAYEARKQFPSERIWITNEIIHNPTVNKRLEEMEVQNIPLEEGKKQFEV 180
           GFCWGVERAVQIAYEARKQFPSE+IWITNEIIHNPTVN++LEEMEV+NIP+++GKKQF+V
Sbjct: 121 GFCWGVERAVQIAYEARKQFPSEKIWITNEIIHNPTVNQKLEEMEVKNIPIDDGKKQFDV 180

Query: 181 VDKGDVVILPAFGAAVDEMLTLSEKNVQIVDTTCPWVSKVWNVVEKHKKGDYTSIIHGKY 240
           V+KGDVV+LPAFGAAVDEML LS+KNVQIVDTTCPWVSKVW  VEKHKKG++TSIIHGKY
Sbjct: 181 VEKGDVVVLPAFGAAVDEMLILSDKNVQIVDTTCPWVSKVWTSVEKHKKGEHTSIIHGKY 240

Query: 241 AHEETVATSSFAGTYIIVKNMKEATYVCDYILGGQLDGSSSTREAFLEKFKYAVSDGFDP 300
           +HEETVAT+SFAG YIIVKNMKEATYVCDYILGG LDGSSST+EAFLE FK AVS GFDP
Sbjct: 241 SHEETVATASFAGKYIIVKNMKEATYVCDYILGGGLDGSSSTKEAFLEIFKLAVSKGFDP 300

Query: 301 DRDLVKTGIANQTTMLKGETEDIGKLVERTMMRKYGVENLNNHFLSFNTICDATQERQDA 360
           D+DL+K GIANQTTMLKGETEDIGKLVERTMM+KYGVEN+NNHF+SFNTICDATQERQDA
Sbjct: 301 DQDLIKVGIANQTTMLKGETEDIGKLVERTMMQKYGVENVNNHFMSFNTICDATQERQDA 360

Query: 361 MYKLVEEKVDLMLVVGGWNSSNTSHLQEIVEERGIPSYWIDSEERIGPGNKISYKLNHGE 420
           MYKLV+EK+DLMLVVGGWNSSNTSHLQEI EER IPSYW+DSE+RIGPGNKISYKL HGE
Sbjct: 361 MYKLVDEKIDLMLVVGGWNSSNTSHLQEIAEERNIPSYWVDSEKRIGPGNKISYKLMHGE 420

Query: 421 LVEKENWLPEGPITIGITSGASTPDKLFLFLGC 451
           LVEKENWLPEGPITIGITSGASTPDK+ L   C
Sbjct: 421 LVEKENWLPEGPITIGITSGASTPDKVILLTSC 450

[Close]

BLAST of Spo03335.1 vs. UniProtKB/TrEMBL
Match: A0A0M4K5E2_MORAL (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Morus alba PE=2 SV=1)

HSP 1 Score: 750.7 bits (1937), Expect = 1.000e-213
Identity = 366/445 (82.25%), Postives = 407/445 (91.46%), Query Frame = 1


		  
Query: 1   MAISPHLSRIITFTELRLPET-QFRPNFHRKPHLSVRCVAGESPTSSSSSAVDSKFDAKK 60
           MAI+  L RI T T+L LPET   R    RKP LS+RC    S  SS + A +S FDAK 
Sbjct: 1   MAIAFQLCRIPTRTDLFLPETFSLRC---RKP-LSLRCSGDSSSASSPAVAAESDFDAKV 60

Query: 61  FHHNLTRSENYNRKGFGHKEETLQLMNSEYTSDIVETLKKNGYEYTWGNVTVKLAESFGF 120
           F  NLTRS+NYNRKGFGHKEETL+LMN EYTSD+++TLK+NG EYTWGNVTVKLAE++GF
Sbjct: 61  FRKNLTRSKNYNRKGFGHKEETLELMNREYTSDVIKTLKENGNEYTWGNVTVKLAEAYGF 120

Query: 121 CWGVERAVQIAYEARKQFPSERIWITNEIIHNPTVNKRLEEMEVQNIPLEEGKKQFEVVD 180
           CWGVERAVQIAYEARKQFP E+IWITNEIIHNPTVNKRLEEM+V+NIP+E+G KQFEVVD
Sbjct: 121 CWGVERAVQIAYEARKQFPDEKIWITNEIIHNPTVNKRLEEMKVENIPIEQGNKQFEVVD 180

Query: 181 KGDVVILPAFGAAVDEMLTLSEKNVQIVDTTCPWVSKVWNVVEKHKKGDYTSIIHGKYAH 240
           KGDVVILPAFGAAV+EMLTLS+KNVQIVDTTCPWVSKVWN VEKHKKG+YTSIIHGKY+H
Sbjct: 181 KGDVVILPAFGAAVEEMLTLSDKNVQIVDTTCPWVSKVWNTVEKHKKGEYTSIIHGKYSH 240

Query: 241 EETVATSSFAGTYIIVKNMKEATYVCDYILGGQLDGSSSTREAFLEKFKYAVSDGFDPDR 300
           EET+AT+SFAGTYIIVKNM+EATYVCDYILGG LDGSSSTREAFLEKFKYAVSDGFDPD+
Sbjct: 241 EETIATASFAGTYIIVKNMQEATYVCDYILGGALDGSSSTREAFLEKFKYAVSDGFDPDK 300

Query: 301 DLVKTGIANQTTMLKGETEDIGKLVERTMMRKYGVENLNNHFLSFNTICDATQERQDAMY 360
           DLVK GIANQTTMLKGETE+IGKL ERTMM+KYGVEN+N+HF+SFNTICDATQERQDAMY
Sbjct: 301 DLVKVGIANQTTMLKGETEEIGKLAERTMMQKYGVENVNDHFISFNTICDATQERQDAMY 360

Query: 361 KLVEEKVDLMLVVGGWNSSNTSHLQEIVEERGIPSYWIDSEERIGPGNKISYKLNHGELV 420
           K+VE+ +DL+LVVGGWNSSNTSHLQEI EERGIPSYWIDSE+RIGPGN+I+YKLNHGELV
Sbjct: 361 KMVEDDLDLILVVGGWNSSNTSHLQEIAEERGIPSYWIDSEQRIGPGNRIAYKLNHGELV 420

Query: 421 EKENWLPEGPITIGITSGASTPDKL 445
           EKENWLP+GP+ IGITSGASTPDK+
Sbjct: 421 EKENWLPKGPLAIGITSGASTPDKV 441

[Close]

BLAST of Spo03335.1 vs. ExPASy Swiss-Prot
Match: ISPH_ORYSJ (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic OS=Oryza sativa subsp. japonica GN=ISPH PE=2 SV=1)

HSP 1 Score: 705.7 bits (1820), Expect = 3.400e-202
Identity = 333/416 (80.05%), Postives = 386/416 (92.79%), Query Frame = 1


		  
Query: 29  RKPHLSVRCVAGESPTSSSSSAVDSKFDAKKFHHNLTRSENYNRKGFGHKEETLQLMNSE 88
           R+   SVRC +  + + S+S+++D+ FD K+F HNLTRS+NYNRKGFGHK+ETL+LM+ E
Sbjct: 24  RRAPSSVRCDSSAASSLSASASLDADFDKKQFRHNLTRSDNYNRKGFGHKKETLELMSQE 83

Query: 89  YTSDIVETLKKNGYEYTWGNVTVKLAESFGFCWGVERAVQIAYEARKQFPSERIWITNEI 148
           YTSD+++TLK+NG ++TWG VTVKLAE++GFCWGVERAVQIAYEARKQFP +RIW+TNEI
Sbjct: 84  YTSDVIKTLKENGNQHTWGPVTVKLAEAYGFCWGVERAVQIAYEARKQFPDDRIWLTNEI 143

Query: 149 IHNPTVNKRLEEMEVQNIPLEEGKKQFEVVDKGDVVILPAFGAAVDEMLTLSEKNVQIVD 208
           IHNPTVNKRLE+M VQNIP++ G K F+VV++GDVV+LPAFGAAV+EM TL+EK VQIVD
Sbjct: 144 IHNPTVNKRLEDMGVQNIPVDAGIKDFDVVEQGDVVVLPAFGAAVEEMYTLNEKKVQIVD 203

Query: 209 TTCPWVSKVWNVVEKHKKGDYTSIIHGKYAHEETVATSSFAGTYIIVKNMKEATYVCDYI 268
           TTCPWVSKVWN+VEKHKKGDYTSIIHGKY+HEETVAT+SFAGTYIIVKN+ EA+YVCDYI
Sbjct: 204 TTCPWVSKVWNMVEKHKKGDYTSIIHGKYSHEETVATASFAGTYIIVKNIAEASYVCDYI 263

Query: 269 LGGQLDGSSSTREAFLEKFKYAVSDGFDPDRDLVKTGIANQTTMLKGETEDIGKLVERTM 328
           LGGQLDGSSST+E FLEKFK AVS GFDPD DLVK GIANQTTMLKGETE+IGKLVE+TM
Sbjct: 264 LGGQLDGSSSTKEEFLEKFKNAVSPGFDPDVDLVKVGIANQTTMLKGETEEIGKLVEKTM 323

Query: 329 MRKYGVENLNNHFLSFNTICDATQERQDAMYKLVEEKVDLMLVVGGWNSSNTSHLQEIVE 388
           MR++GVEN+N+HF++FNTICDATQERQDAMY+LV+EKVDL+LVVGGWNSSNTSHLQEI E
Sbjct: 324 MRRFGVENVNDHFIAFNTICDATQERQDAMYQLVKEKVDLILVVGGWNSSNTSHLQEIGE 383

Query: 389 ERGIPSYWIDSEERIGPGNKISYKLNHGELVEKENWLPEGPITIGITSGASTPDKL 445
             GIPSYWIDSE+RIGPGNKISYKLNHGELVEKENWLPEGPITIG+TSGASTPDK+
Sbjct: 384 LSGIPSYWIDSEQRIGPGNKISYKLNHGELVEKENWLPEGPITIGVTSGASTPDKV 439

[Close]

BLAST of Spo03335.1 vs. ExPASy Swiss-Prot
Match: ISPH_ARATH (4-hydroxy-3-methylbut-2-enyl diphosphate reductase, chloroplastic OS=Arabidopsis thaliana GN=ISPH PE=2 SV=1)

HSP 1 Score: 702.2 bits (1811), Expect = 3.700e-201
Identity = 339/451 (75.17%), Postives = 390/451 (86.47%), Query Frame = 1


		  
Query: 1   MAISPHLSRIITFTELRLPETQFRPN-------FHRKPHLSVRCVAGESPTSSSSSAVDS 60
           MA++   SR+        P+T  R N         R+  LSVRC +G+    S S  +DS
Sbjct: 1   MAVALQFSRLCV-----RPDTFVRENHLSGSGSLRRRKALSVRCSSGDENAPSPSVVMDS 60

Query: 61  KFDAKKFHHNLTRSENYNRKGFGHKEETLQLMNSEYTSDIVETLKKNGYEYTWGNVTVKL 120
            FDAK F  NLTRS+NYNRKGFGHKEETL+LMN EYTSDI+ETLK NGY Y+WG+VTVKL
Sbjct: 61  DFDAKVFRKNLTRSDNYNRKGFGHKEETLKLMNREYTSDILETLKTNGYTYSWGDVTVKL 120

Query: 121 AESFGFCWGVERAVQIAYEARKQFPSERIWITNEIIHNPTVNKRLEEMEVQNIPLEEGKK 180
           A+++GFCWGVERAVQIAYEARKQFP ER+WITNEIIHNPTVNKRLE+M+V+ IP+E+ KK
Sbjct: 121 AKAYGFCWGVERAVQIAYEARKQFPEERLWITNEIIHNPTVNKRLEDMDVKIIPVEDSKK 180

Query: 181 QFEVVDKGDVVILPAFGAAVDEMLTLSEKNVQIVDTTCPWVSKVWNVVEKHKKGDYTSII 240
           QF+VV+K DVVILPAFGA VDEM  L++K VQIVDTTCPWV+KVWN VEKHKKG+YTS+I
Sbjct: 181 QFDVVEKDDVVILPAFGAGVDEMYVLNDKKVQIVDTTCPWVTKVWNTVEKHKKGEYTSVI 240

Query: 241 HGKYAHEETVATSSFAGTYIIVKNMKEATYVCDYILGGQLDGSSSTREAFLEKFKYAVSD 300
           HGKY HEET+AT+SFAG YIIVKNMKEA YVCDYILGGQ DGSSST+E F+EKFKYA+S 
Sbjct: 241 HGKYNHEETIATASFAGKYIIVKNMKEANYVCDYILGGQYDGSSSTKEEFMEKFKYAISK 300

Query: 301 GFDPDRDLVKTGIANQTTMLKGETEDIGKLVERTMMRKYGVENLNNHFLSFNTICDATQE 360
           GFDPD DLVK GIANQTTMLKGETE+IG+L+E TMMRKYGVEN++ HF+SFNTICDATQE
Sbjct: 301 GFDPDNDLVKVGIANQTTMLKGETEEIGRLLETTMMRKYGVENVSGHFISFNTICDATQE 360

Query: 361 RQDAMYKLVEEKVDLMLVVGGWNSSNTSHLQEIVEERGIPSYWIDSEERIGPGNKISYKL 420
           RQDA+Y+LVEEK+DLMLVVGGWNSSNTSHLQEI E RGIPSYWIDSE+RIGPGNKI+YKL
Sbjct: 361 RQDAIYELVEEKIDLMLVVGGWNSSNTSHLQEISEARGIPSYWIDSEKRIGPGNKIAYKL 420

Query: 421 NHGELVEKENWLPEGPITIGITSGASTPDKL 445
           ++GELVEKEN+LP+GPITIG+TSGASTPDK+
Sbjct: 421 HYGELVEKENFLPKGPITIGVTSGASTPDKV 446

[Close]

BLAST of Spo03335.1 vs. ExPASy Swiss-Prot
Match: ISPH_CYAP4 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Cyanothece sp. (strain PCC 7425 / ATCC 29141) GN=ispH PE=3 SV=1)

HSP 1 Score: 531.6 bits (1368), Expect = 8.700e-150
Identity = 256/390 (65.64%), Postives = 311/390 (79.74%), Query Frame = 1


		  
Query: 56  DAKKFHHNLTRSENYNRKGFGHKEETLQLMNSEYTSDIVETLKKNGYEYTWGNVTVKLAE 115
           D K F  +L  S  Y+RKGFGH+ E  +++  EY S +++ +++NG  +  G VT++LAE
Sbjct: 2   DTKAFKRSLHSSNQYHRKGFGHEAEVTEMLRFEYQSSLIQQIRENGNRFQRGEVTIQLAE 61

Query: 116 SFGFCWGVERAVQIAYEARKQFPSERIWITNEIIHNPTVNKRLEEMEVQNIPLEE-GKKQ 175
           +FGFCWGVERAV +AYE R+ FP+ERIWITNEIIHNP+VN+ L EM+V+ IP++E G+K 
Sbjct: 62  AFGFCWGVERAVALAYETRQHFPTERIWITNEIIHNPSVNQNLREMQVEFIPVDEQGQKD 121

Query: 176 FEVVDKGDVVILPAFGAAVDEMLTLSEKNVQIVDTTCPWVSKVWNVVEKHKKGDYTSIIH 235
           F VV   DVVILPAFGA+V EM  L++K  +I+DTTCPWVSKVWN VEKHKKG YTSIIH
Sbjct: 122 FSVVAGEDVVILPAFGASVQEMQLLNDKGCKIMDTTCPWVSKVWNSVEKHKKGSYTSIIH 181

Query: 236 GKYAHEETVATSSFAGTYIIVKNMKEATYVCDYILGGQLDGSSSTREAFLEKFKYAVSDG 295
           GKY HEET+ATSS+A TY+IV ++KEA YVC+YIL G        RE FL KF++A S  
Sbjct: 182 GKYQHEETIATSSYAATYLIVLDLKEAEYVCNYILNG------GDREEFLHKFRHAYSPD 241

Query: 296 FDPDRDLVKTGIANQTTMLKGETEDIGKLVERTMMRKYGVENLNNHFLSFNTICDATQER 355
           FDPDRDLV+ GIANQTTMLKGETE IGKL ERT+MRKYG + +N HFLSFNTICDATQER
Sbjct: 242 FDPDRDLVRVGIANQTTMLKGETEQIGKLFERTLMRKYGPDQINQHFLSFNTICDATQER 301

Query: 356 QDAMYKLVEEKVDLMLVVGGWNSSNTSHLQEIVEERGIPSYWIDSEERIGPGNKISYKLN 415
           QDAM+KLVE  +DLM+V+GG+NSSNT+HLQEI  ER IPSY IDS +RIGPGN++ +K  
Sbjct: 302 QDAMFKLVEYPLDLMVVIGGFNSSNTTHLQEISIEREIPSYHIDSADRIGPGNRVEHKPL 361

Query: 416 HGELVEKENWLPEGPITIGITSGASTPDKL 445
           H  L  KENWLPEGPI +GITSGASTPDK+
Sbjct: 362 HRPLEIKENWLPEGPIVVGITSGASTPDKV 385

[Close]

BLAST of Spo03335.1 vs. ExPASy Swiss-Prot
Match: ISPH_CYAA5 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Cyanothece sp. (strain ATCC 51142) GN=ispH PE=3 SV=1)

HSP 1 Score: 531.2 bits (1367), Expect = 1.100e-149
Identity = 255/389 (65.55%), Postives = 310/389 (79.69%), Query Frame = 1


		  
Query: 56  DAKKFHHNLTRSENYNRKGFGHKEETLQLMNSEYTSDIVETLKKNGYEYTWGNVTVKLAE 115
           D K F  +L +S NY+RKGFGH+ E +  MN+EY S +++ +++N Y++  G+VT+KLAE
Sbjct: 2   DTKAFKRSLQQSNNYHRKGFGHETEVMGTMNTEYQSSLIQKIRENNYQWQEGDVTIKLAE 61

Query: 116 SFGFCWGVERAVQIAYEARKQFPSERIWITNEIIHNPTVNKRLEEMEVQNIPLEEGKKQF 175
           +FGFCWGVERAV +AYE R+ FP E+IWITNEIIHNP+VN+RL EM V  I +  G K F
Sbjct: 62  AFGFCWGVERAVAMAYETRQHFPQEKIWITNEIIHNPSVNQRLLEMNVGFIEVINGSKDF 121

Query: 176 EVVDKGDVVILPAFGAAVDEMLTLSEKNVQIVDTTCPWVSKVWNVVEKHKKGDYTSIIHG 235
            VV+ GDVVILPAFGA+V EM  L++K   IVDTTCPWVSKVWN VEKHKK DYTSIIHG
Sbjct: 122 SVVEAGDVVILPAFGASVTEMQLLNDKGCTIVDTTCPWVSKVWNSVEKHKKKDYTSIIHG 181

Query: 236 KYAHEETVATSSFAGTYIIVKNMKEATYVCDYILGGQLDGSSSTREAFLEKFKYAVSDGF 295
           KY HEETVATSSFAGTY++V N+KEA YVCDYIL G        ++ FLEKFK A S+GF
Sbjct: 182 KYKHEETVATSSFAGTYLVVLNLKEAQYVCDYILNG------GNKDEFLEKFKNAHSEGF 241

Query: 296 DPDRDLVKTGIANQTTMLKGETEDIGKLVERTMMRKYGVENLNNHFLSFNTICDATQERQ 355
           DPD+DL++ GIANQTTMLK ETE IGKL E TM++KYG   LN HF+SFNTICDATQERQ
Sbjct: 242 DPDKDLIRLGIANQTTMLKSETEQIGKLFETTMLKKYGPTELNEHFMSFNTICDATQERQ 301

Query: 356 DAMYKLVEEKVDLMLVVGGWNSSNTSHLQEIVEERGIPSYWIDSEERIGPGNKISYKLNH 415
           DAM  LVEE+VD+M+V+GG+NSSNT+HLQEI  E+GI SY IDS++RI PGNKI +K   
Sbjct: 302 DAMLNLVEEEVDVMVVIGGFNSSNTTHLQEISIEKGIDSYHIDSDKRILPGNKIEHKPLE 361

Query: 416 GELVEKENWLPEGPITIGITSGASTPDKL 445
            ++  KENWLP+G I +G+TSGASTPDK+
Sbjct: 362 KDIETKENWLPDGKIIVGVTSGASTPDKV 384

[Close]

BLAST of Spo03335.1 vs. ExPASy Swiss-Prot
Match: ISPH_THEEB (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Thermosynechococcus elongatus (strain BP-1) GN=ispH PE=3 SV=1)

HSP 1 Score: 528.1 bits (1359), Expect = 9.600e-149
Identity = 257/389 (66.07%), Postives = 304/389 (78.15%), Query Frame = 1


		  
Query: 56  DAKKFHHNLTRSENYNRKGFGHKEETLQLMNSEYTSDIVETLKKNGYEYTWGNVTVKLAE 115
           D + F  +L  SENY+RKGFGH EE  Q +  EY S +++ ++ NGY +  G+VT++LAE
Sbjct: 2   DTRAFKRSLHSSENYHRKGFGHGEEVNQQLQGEYQSSLIQQIRANGYRWQQGDVTIRLAE 61

Query: 116 SFGFCWGVERAVQIAYEARKQFPSERIWITNEIIHNPTVNKRLEEMEVQNIPLEEGKKQF 175
           +FGFCWGVERAV +AYE R  FP+ERIWITNEIIHNP+VN+RL +M V+ IP+  G K F
Sbjct: 62  AFGFCWGVERAVALAYETRTHFPTERIWITNEIIHNPSVNERLRQMAVEFIPVVNGVKDF 121

Query: 176 EVVDKGDVVILPAFGAAVDEMLTLSEKNVQIVDTTCPWVSKVWNVVEKHKKGDYTSIIHG 235
             V  GDVVILPAFGA+V EM  L+E+   IVDTTCPWVSKVW+ VEKHKK  +TSIIHG
Sbjct: 122 SGVRPGDVVILPAFGASVQEMQLLNERGCTIVDTTCPWVSKVWHSVEKHKKVSFTSIIHG 181

Query: 236 KYAHEETVATSSFAGTYIIVKNMKEATYVCDYILGGQLDGSSSTREAFLEKFKYAVSDGF 295
           KY HEET+ATSSFAGTY+IV N++EA YVCDYIL G        R AF+ KF  A S GF
Sbjct: 182 KYNHEETIATSSFAGTYLIVLNLEEARYVCDYILHG------GDRAAFMAKFAKACSPGF 241

Query: 296 DPDRDLVKTGIANQTTMLKGETEDIGKLVERTMMRKYGVENLNNHFLSFNTICDATQERQ 355
           DPDRDLV+ GIANQTTMLKGETE IGKL ERTM++KYG + LN HF+SFNTICDATQERQ
Sbjct: 242 DPDRDLVRVGIANQTTMLKGETEQIGKLFERTMIQKYGPDRLNEHFMSFNTICDATQERQ 301

Query: 356 DAMYKLVEEKVDLMLVVGGWNSSNTSHLQEIVEERGIPSYWIDSEERIGPGNKISYKLNH 415
           DAM  LV+E +DLM+V+GG+NSSNT+HLQEI  E GIPSY IDS +RIGPGN+I +K  H
Sbjct: 302 DAMLSLVKEPLDLMVVIGGYNSSNTTHLQEIAIEHGIPSYHIDSADRIGPGNRIEHKPLH 361

Query: 416 GELVEKENWLPEGPITIGITSGASTPDKL 445
                 ENWLP+ PITIGITSGASTPDK+
Sbjct: 362 QNPTVAENWLPDRPITIGITSGASTPDKV 384

[Close]

BLAST of Spo03335.1 vs. TAIR (Arabidopsis)
Match: AT4G34350.1 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase)

HSP 1 Score: 702.2 bits (1811), Expect = 2.100e-202
Identity = 339/451 (75.17%), Postives = 390/451 (86.47%), Query Frame = 1


		  
Query: 1   MAISPHLSRIITFTELRLPETQFRPN-------FHRKPHLSVRCVAGESPTSSSSSAVDS 60
           MA++   SR+        P+T  R N         R+  LSVRC +G+    S S  +DS
Sbjct: 1   MAVALQFSRLCV-----RPDTFVRENHLSGSGSLRRRKALSVRCSSGDENAPSPSVVMDS 60

Query: 61  KFDAKKFHHNLTRSENYNRKGFGHKEETLQLMNSEYTSDIVETLKKNGYEYTWGNVTVKL 120
            FDAK F  NLTRS+NYNRKGFGHKEETL+LMN EYTSDI+ETLK NGY Y+WG+VTVKL
Sbjct: 61  DFDAKVFRKNLTRSDNYNRKGFGHKEETLKLMNREYTSDILETLKTNGYTYSWGDVTVKL 120

Query: 121 AESFGFCWGVERAVQIAYEARKQFPSERIWITNEIIHNPTVNKRLEEMEVQNIPLEEGKK 180
           A+++GFCWGVERAVQIAYEARKQFP ER+WITNEIIHNPTVNKRLE+M+V+ IP+E+ KK
Sbjct: 121 AKAYGFCWGVERAVQIAYEARKQFPEERLWITNEIIHNPTVNKRLEDMDVKIIPVEDSKK 180

Query: 181 QFEVVDKGDVVILPAFGAAVDEMLTLSEKNVQIVDTTCPWVSKVWNVVEKHKKGDYTSII 240
           QF+VV+K DVVILPAFGA VDEM  L++K VQIVDTTCPWV+KVWN VEKHKKG+YTS+I
Sbjct: 181 QFDVVEKDDVVILPAFGAGVDEMYVLNDKKVQIVDTTCPWVTKVWNTVEKHKKGEYTSVI 240

Query: 241 HGKYAHEETVATSSFAGTYIIVKNMKEATYVCDYILGGQLDGSSSTREAFLEKFKYAVSD 300
           HGKY HEET+AT+SFAG YIIVKNMKEA YVCDYILGGQ DGSSST+E F+EKFKYA+S 
Sbjct: 241 HGKYNHEETIATASFAGKYIIVKNMKEANYVCDYILGGQYDGSSSTKEEFMEKFKYAISK 300

Query: 301 GFDPDRDLVKTGIANQTTMLKGETEDIGKLVERTMMRKYGVENLNNHFLSFNTICDATQE 360
           GFDPD DLVK GIANQTTMLKGETE+IG+L+E TMMRKYGVEN++ HF+SFNTICDATQE
Sbjct: 301 GFDPDNDLVKVGIANQTTMLKGETEEIGRLLETTMMRKYGVENVSGHFISFNTICDATQE 360

Query: 361 RQDAMYKLVEEKVDLMLVVGGWNSSNTSHLQEIVEERGIPSYWIDSEERIGPGNKISYKL 420
           RQDA+Y+LVEEK+DLMLVVGGWNSSNTSHLQEI E RGIPSYWIDSE+RIGPGNKI+YKL
Sbjct: 361 RQDAIYELVEEKIDLMLVVGGWNSSNTSHLQEISEARGIPSYWIDSEKRIGPGNKIAYKL 420

Query: 421 NHGELVEKENWLPEGPITIGITSGASTPDKL 445
           ++GELVEKEN+LP+GPITIG+TSGASTPDK+
Sbjct: 421 HYGELVEKENFLPKGPITIGVTSGASTPDKV 446

The following BLAST results are available for this feature:

BLAST of Spo03335.1 vs. NCBI nr
BLAST of Spo03335.1 vs. UniProtKB/TrEMBL
BLAST of Spo03335.1 vs. ExPASy Swiss-Prot
BLAST of Spo03335.1 vs. TAIR (Arabidopsis)

BLAST of Spo03335.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902186035\|gb\|KNA10609.1\|	1.8e-259	99.7	hypothetical protein SOVF_1427...	[more]
gi\|731358793\|ref\|XP_010690939.1\|	2.5e-229	88.0	PREDICTED: 4-hydroxy-3-methylb...	[more]
gi\|747082064\|ref\|XP_011088335.1\|	1.0e-214	81.8	PREDICTED: 4-hydroxy-3-methylb...	[more]
gi\|703097851\|ref\|XP_010096225.1\|	1.3e-214	83.7	4-hydroxy-3-methylbut-2-enyl d...	[more]
gi\|747090179\|ref\|XP_011092767.1\|	1.7e-214	83.4	PREDICTED: 4-hydroxy-3-methylb...	[more]

BLAST of Spo03335.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9QTN2_SPIOL	1.2e-259	99.7	Uncharacterized protein OS=Spi...	[more]
A0A0J8BK59_BETVU	1.7e-229	88.0	Uncharacterized protein OS=Bet...	[more]
W9R118_9ROSA	9.2e-215	83.7	4-hydroxy-3-methylbut-2-enyl d...	[more]
A0A022RCG7_ERYGU	3.5e-214	81.2	Uncharacterized protein OS=Ery...	[more]
A0A0M4K5E2_MORAL	1.0e-213	82.2	4-hydroxy-3-methylbut-2-enyl d...	[more]

BLAST of Spo03335.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
ISPH_ORYSJ	3.4e-202	80.0	4-hydroxy-3-methylbut-2-enyl d...	[more]
ISPH_ARATH	3.7e-201	75.1	4-hydroxy-3-methylbut-2-enyl d...	[more]
ISPH_CYAP4	8.7e-150	65.6	4-hydroxy-3-methylbut-2-enyl d...	[more]
ISPH_CYAA5	1.1e-149	65.5	4-hydroxy-3-methylbut-2-enyl d...	[more]
ISPH_THEEB	9.6e-149	66.0	4-hydroxy-3-methylbut-2-enyl d...	[more]

BLAST of Spo03335.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 1


Match Name	E-value	Identity	Description
AT4G34350.1	2.1e-202	75.1	4-hydroxy-3-methylbut-2-enyl d...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003451	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	HAMAP	MF_00191	IspH	coord: 109..456 score: 27
IPR003451	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	PFAM	PF02401	LYTB	coord: 111..444 score: 6.5
IPR003451	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	TIGRFAMs	TIGR00216	TIGR00216	coord: 111..444 score: 1.2
None	No IPR available	PANTHER	PTHR31619	FAMILY NOT NAMED	coord: 1..443 score:
None	No IPR available	PANTHER	PTHR31619:SF2	4-HYDROXY-3-METHYLBUT-2-ENYL DIPHOSPHATE REDUCTASE, CHLOROPLASTIC	coord: 1..443 score:

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0050992	dimethylallyl diphosphate biosynthetic process
biological_process	GO:0019288	isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway
biological_process	GO:0055114	oxidation-reduction process
molecular_function	GO:0051745	4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity
molecular_function	GO:0046872	metal ion binding