Spo04457.1 (mRNA) | SpinachBase

Overview


Name	Spo04457.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	Calcium-dependent lipid-binding (CaLB domain) family protein
Location	chr3 : 2984222 .. 2985136 (-)
Sequence length	915

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGTCAAGATTTGAGGTAGAGGTGACCTTGTCATCAGCCAAGGACCTCAAGAACGTTAACTGGCGTCACGGCCCGATAAAGCCGTACGCCGTCATGTGGTTCGACCCGGCCCGTAAAATAACGAGTCGGGTCGACGAGGAGGGCGACACCACCCCACATTGGAACCAAACACTCGTTATCCCCCTCGATCGCCCCATAGAAGACGCCACCCTCCACATCGACATCGTCCACGCGTACGCCGACGAGGACACCAAGCCGCTCATCGGCTCCGCTAAACTGCCGCTGAGAGAGGTGGTTGATGACGTGGGTGTTGGCGGTCGGCTTAATCGGAGCTTAAAGCTAAAACGGCCATCAGGAAGGCCGCATGGGAAGGTTGAGGTTGAGGTGGTTGTACGTCAGCCACGGTACCACGCCCCTGATCCGTACGGTGCGCCACCGTATGGAGTCGCATCTAAGGATGCAGGATACGGTGGCGCGCCCTATATGGCCCCACCAACAGGGTATCCCCACATGGCACCACCACCTGGTGGCTCTGGGGGCTATCCTTACGGACAGCCCGCTTACGCGCCGCCTTCATACGGACAACCAGCTTACGCGCCGCCGTCATACGGGCAGCCAGCTTACGGGCAGCCTGGATACGCGCAGCCGAGTTACGGGCAGCCAGGCGGTGGTGGGTACGGGTATGGGCAGGTGGCGGAGGAGAAGAAGAAGGGTGGTAAATTTGGAGGGATGGGGACAGGATTGGCTGTGGGTGCGGTTGCAGGGGCGTTGGGTGGACTTGCATTAGTTGAAGGGTTTGATAAATTGGAAGATCATATTGCCGATGAGGCGGCGGAGAAGGTGGAGGATGATCTTGCTGATGGTGGTTATGATGATGGGGATTATGGCGGTGGTGATGATGATGGATGGTAG

mRNA sequence

ATGGCGTCAAGATTTGAGGTAGAGGTGACCTTGTCATCAGCCAAGGACCTCAAGAACGTTAACTGGCGTCACGGCCCGATAAAGCCGTACGCCGTCATGTGGTTCGACCCGGCCCGTAAAATAACGAGTCGGGTCGACGAGGAGGGCGACACCACCCCACATTGGAACCAAACACTCGTTATCCCCCTCGATCGCCCCATAGAAGACGCCACCCTCCACATCGACATCGTCCACGCGTACGCCGACGAGGACACCAAGCCGCTCATCGGCTCCGCTAAACTGCCGCTGAGAGAGGTGGTTGATGACGTGGGTGTTGGCGGTCGGCTTAATCGGAGCTTAAAGCTAAAACGGCCATCAGGAAGGCCGCATGGGAAGGTTGAGGTTGAGGTGGTTGTACGTCAGCCACGGTACCACGCCCCTGATCCGTACGGTGCGCCACCGTATGGAGTCGCATCTAAGGATGCAGGATACGGTGGCGCGCCCTATATGGCCCCACCAACAGGGTATCCCCACATGGCACCACCACCTGGTGGCTCTGGGGGCTATCCTTACGGACAGCCCGCTTACGCGCCGCCTTCATACGGACAACCAGCTTACGCGCCGCCGTCATACGGGCAGCCAGCTTACGGGCAGCCTGGATACGCGCAGCCGAGTTACGGGCAGCCAGGCGGTGGTGGGTACGGGTATGGGCAGGTGGCGGAGGAGAAGAAGAAGGGTGGTAAATTTGGAGGGATGGGGACAGGATTGGCTGTGGGTGCGGTTGCAGGGGCGTTGGGTGGACTTGCATTAGTTGAAGGGTTTGATAAATTGGAAGATCATATTGCCGATGAGGCGGCGGAGAAGGTGGAGGATGATCTTGCTGATGGTGGTTATGATGATGGGGATTATGGCGGTGGTGATGATGATGGATGGTAG

Coding sequence (CDS)

ATGGCGTCAAGATTTGAGGTAGAGGTGACCTTGTCATCAGCCAAGGACCTCAAGAACGTTAACTGGCGTCACGGCCCGATAAAGCCGTACGCCGTCATGTGGTTCGACCCGGCCCGTAAAATAACGAGTCGGGTCGACGAGGAGGGCGACACCACCCCACATTGGAACCAAACACTCGTTATCCCCCTCGATCGCCCCATAGAAGACGCCACCCTCCACATCGACATCGTCCACGCGTACGCCGACGAGGACACCAAGCCGCTCATCGGCTCCGCTAAACTGCCGCTGAGAGAGGTGGTTGATGACGTGGGTGTTGGCGGTCGGCTTAATCGGAGCTTAAAGCTAAAACGGCCATCAGGAAGGCCGCATGGGAAGGTTGAGGTTGAGGTGGTTGTACGTCAGCCACGGTACCACGCCCCTGATCCGTACGGTGCGCCACCGTATGGAGTCGCATCTAAGGATGCAGGATACGGTGGCGCGCCCTATATGGCCCCACCAACAGGGTATCCCCACATGGCACCACCACCTGGTGGCTCTGGGGGCTATCCTTACGGACAGCCCGCTTACGCGCCGCCTTCATACGGACAACCAGCTTACGCGCCGCCGTCATACGGGCAGCCAGCTTACGGGCAGCCTGGATACGCGCAGCCGAGTTACGGGCAGCCAGGCGGTGGTGGGTACGGGTATGGGCAGGTGGCGGAGGAGAAGAAGAAGGGTGGTAAATTTGGAGGGATGGGGACAGGATTGGCTGTGGGTGCGGTTGCAGGGGCGTTGGGTGGACTTGCATTAGTTGAAGGGTTTGATAAATTGGAAGATCATATTGCCGATGAGGCGGCGGAGAAGGTGGAGGATGATCTTGCTGATGGTGGTTATGATGATGGGGATTATGGCGGTGGTGATGATGATGGATGGTAG

Protein sequence

MASRFEVEVTLSSAKDLKNVNWRHGPIKPYAVMWFDPARKITSRVDEEGDTTPHWNQTLVIPLDRPIEDATLHIDIVHAYADEDTKPLIGSAKLPLREVVDDVGVGGRLNRSLKLKRPSGRPHGKVEVEVVVRQPRYHAPDPYGAPPYGVASKDAGYGGAPYMAPPTGYPHMAPPPGGSGGYPYGQPAYAPPSYGQPAYAPPSYGQPAYGQPGYAQPSYGQPGGGGYGYGQVAEEKKKGGKFGGMGTGLAVGAVAGALGGLALVEGFDKLEDHIADEAAEKVEDDLADGGYDDGDYGGGDDDGW

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo04457	Spo04457	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo04457.1	Spo04457.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo04457.1.CDS.1	Spo04457.1.CDS.1	CDS

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo04457.1.exon.1	Spo04457.1.exon.1	exon

Homology

[Close]

BLAST of Spo04457.1 vs. NCBI nr
Match: gi|902236244|gb|KNA24283.1| (hypothetical protein SOVF_016910 [Spinacia oleracea])

HSP 1 Score: 606.3 bits (1562), Expect = 2.900e-170
Identity = 303/304 (99.67%), Postives = 303/304 (99.67%), Query Frame = 1


		  
Query: 1   MASRFEVEVTLSSAKDLKNVNWRHGPIKPYAVMWFDPARKITSRVDEEGDTTPHWNQTLV 60
           MASRFEVEVTLSSAKDLKNVNWRHGPIKPYAVMWFDPARKITSRVDEEGDTTPHWNQTLV
Sbjct: 1   MASRFEVEVTLSSAKDLKNVNWRHGPIKPYAVMWFDPARKITSRVDEEGDTTPHWNQTLV 60

Query: 61  IPLDRPIEDATLHIDIVHAYADEDTKPLIGSAKLPLREVVDDVGVGGRLNRSLKLKRPSG 120
           IPLDRPIEDATLHIDIVHAYADEDTKPLIGSAKLPLREVVDDVGVGGRLNRSLKLKRPSG
Sbjct: 61  IPLDRPIEDATLHIDIVHAYADEDTKPLIGSAKLPLREVVDDVGVGGRLNRSLKLKRPSG 120

Query: 121 RPHGKVEVEVVVRQPRYHAPDPYGAPPYGVASKDAGYGGAPYMAPPTGYPHMAPPPGGSG 180
           RPHGKVEVEVVVRQPRYH PDPYGAPPYGVASKDAGYGGAPYMAPPTGYPHMAPPPGGSG
Sbjct: 121 RPHGKVEVEVVVRQPRYHTPDPYGAPPYGVASKDAGYGGAPYMAPPTGYPHMAPPPGGSG 180

Query: 181 GYPYGQPAYAPPSYGQPAYAPPSYGQPAYGQPGYAQPSYGQPGGGGYGYGQVAEEKKKGG 240
           GYPYGQPAYAPPSYGQPAYAPPSYGQPAYGQPGYAQPSYGQPGGGGYGYGQVAEEKKKGG
Sbjct: 181 GYPYGQPAYAPPSYGQPAYAPPSYGQPAYGQPGYAQPSYGQPGGGGYGYGQVAEEKKKGG 240

Query: 241 KFGGMGTGLAVGAVAGALGGLALVEGFDKLEDHIADEAAEKVEDDLADGGYDDGDYGGGD 300
           KFGGMGTGLAVGAVAGALGGLALVEGFDKLEDHIADEAAEKVEDDLADGGYDDGDYGGGD
Sbjct: 241 KFGGMGTGLAVGAVAGALGGLALVEGFDKLEDHIADEAAEKVEDDLADGGYDDGDYGGGD 300

Query: 301 DDGW 305
           DDGW
Sbjct: 301 DDGW 304

[Close]

BLAST of Spo04457.1 vs. NCBI nr
Match: gi|747059492|ref|XP_011076131.1| (PREDICTED: WW domain-containing protein C11B10.08 [Sesamum indicum])

HSP 1 Score: 340.5 bits (872), Expect = 3.000e-90
Identity = 194/298 (65.10%), Postives = 221/298 (74.16%), Query Frame = 1


		  
Query: 1   MASRFEVEVTLSSAKDLKNVNWRHGPIKPYAVMWFDPARKITSRVDEEGDTTPHWNQTLV 60
           M SR+EVEVT++SAKDLKNVNWRHG +KPYAV+W DP  K ++RVDE+GDT+P+W + +V
Sbjct: 1   MGSRYEVEVTVTSAKDLKNVNWRHGQLKPYAVLWVDPKNKCSTRVDEDGDTSPYWEEKVV 60

Query: 61  IPLDRPIEDATLHIDIVHAYADEDTKPLIGSAKLPLREVVDDVGVGGRLNRSLKLKRPSG 120
           IP + PIED+TL+IDIVHA   EDTKPLIGSAKLPLR+VVDD G+G R+ R L+LKRPSG
Sbjct: 61  IPFNSPIEDSTLYIDIVHANDAEDTKPLIGSAKLPLRDVVDDAGMGARVERKLELKRPSG 120

Query: 121 RPHGKVEVEVVVRQPRYHAPDPYGAPPYGVASKDAGYGGAPYMAPPTGYPHMAPPPGGSG 180
           RP GK+EV V VR+PRY APDPY APPYGV              PP    + APP G   
Sbjct: 121 RPQGKLEVSVSVREPRYRAPDPYYAPPYGV-------------PPPATRDYPAPPYGNQ- 180

Query: 181 GYPYGQPAYAPPSYGQPAY-APPSYGQPAYGQPGYAQPS-YGQPGGGGYGYGQVAEEKKK 240
            Y    PAYA P  G PAY APPSYGQP+YGQP Y Q S YG+PG G  GY    EEKKK
Sbjct: 181 -YYGAPPAYAAPPSGYPAYGAPPSYGQPSYGQPAYGQGSYYGEPGYGQQGY---VEEKKK 240

Query: 241 GGKFGGMGTGLAVGAVAGALGGLALVEGFDKLEDHIADEAAEKVEDDLADGGYDDGDY 297
             KFGGMGTGLAVGAVAGALGGLAL EG D LED IAD+AAE+VEDDL   GYD  D+
Sbjct: 241 -SKFGGMGTGLAVGAVAGALGGLALAEGVDALEDKIADDAAERVEDDL---GYDGDDF 276

[Close]

BLAST of Spo04457.1 vs. NCBI nr
Match: gi|661867246|emb|CDP21817.1| (unnamed protein product [Coffea canephora])

HSP 1 Score: 337.0 bits (863), Expect = 3.300e-89
Identity = 193/308 (62.66%), Postives = 224/308 (72.73%), Query Frame = 1


		  
Query: 1   MASRFEVEVTLSSAKDLKNVNWRHGPIKPYAVMWFDPARKITSRVDEEGDTTPHWNQTLV 60
           M SR+EVEV ++SAKDLKNVNWRHGP+KPYAV+W DP  K ++RVD+EGDT+P+W +TLV
Sbjct: 1   MGSRYEVEVAITSAKDLKNVNWRHGPLKPYAVVWVDPKAKCSTRVDDEGDTSPYWEETLV 60

Query: 61  IPLDRPIEDATLHIDIVHAYADEDTKPLIGSAKLPLREVVDDVGVGGRLNRSLKLKRPSG 120
           IP D PIED+TL ID+VHA A EDTKPLIGSA+L L +VVD+ G+G R+ R L+LKRPSG
Sbjct: 61  IPFDSPIEDSTLLIDVVHAKAAEDTKPLIGSARLRLIDVVDEAGLGRRVERKLELKRPSG 120

Query: 121 RPHGKVEVEVVVRQ-PRYHAPDPYGAPPYGV---ASKDAG----YGGAPYMAPPTGYPHM 180
           RPHGK++V+V VR+ PRY APDPY APPYGV    S+D      YG  PY APP    + 
Sbjct: 121 RPHGKLQVQVSVREPPRYRAPDPYYAPPYGVPQPGSRDYAAPQPYGNPPYGAPPAS-AYA 180

Query: 181 APPPGGSGGYPYGQPAYAPPSYGQPAYAPPSYGQPAYGQPGYAQPSYGQPGGGGYGYGQV 240
           APP     GYPY  P         PA  PPSYGQP+YGQP Y QPSYGQP         V
Sbjct: 181 APP----AGYPYSAP---------PAAPPPSYGQPSYGQPAYGQPSYGQP---------V 240

Query: 241 AEEKKKGGKFGGMGTGLAVGAVAGALGGLALVEGFDKLEDHIADEAAEKVEDDLADGGYD 300
             E+KK  KFGGMGTGLAVGAVAGALGGLAL EG D  +D IAD+AAEKVE+D+   GYD
Sbjct: 241 YGEEKKKSKFGGMGTGLAVGAVAGALGGLALFEGIDYAKDKIADDAAEKVEEDI---GYD 280

[Close]

BLAST of Spo04457.1 vs. NCBI nr
Match: gi|698571473|ref|XP_009774894.1| (PREDICTED: uncharacterized protein LOC104224872 [Nicotiana sylvestris])

HSP 1 Score: 333.6 bits (854), Expect = 3.700e-88
Identity = 184/303 (60.73%), Postives = 217/303 (71.62%), Query Frame = 1


		  
Query: 1   MASRFEVEVTLSSAKDLKNVNWRHGPIKPYAVMWFDPARKITSRVDEEGDTTPHWNQTLV 60
           M SR+EVEV ++SA DLKNV WR+GP+KPYAV+W DP  K +++VD++GDT+P+WN+ LV
Sbjct: 1   MGSRYEVEVQITSANDLKNVTWRYGPLKPYAVVWVDPNAKCSTKVDDDGDTSPYWNEKLV 60

Query: 61  IPLDRPIEDATLHIDIVHAYADEDTKPLIGSAKLPLREVVDDVGVGGRLNRSLKLKRPSG 120
           +PL+  I+D+TL++DIVHA A EDTKPLIGSAKLPL +V+DDVG+GG L  +L+LKRPSG
Sbjct: 61  VPLNSSIDDSTLYVDIVHANAVEDTKPLIGSAKLPLSKVIDDVGIGGELECTLQLKRPSG 120

Query: 121 RPHGKVEVEVVVRQPRYHAPDPYGAPPYGVASKDAGYG---GAPYMAPPTGYPHMAPPPG 180
           RP GKV+VEV VR+PRY APD Y APPYGV      YG   GAPY APP+GYP+ A  P 
Sbjct: 121 RPQGKVQVEVSVREPRYRAPDAYYAPPYGVPPPSQPYGRDYGAPYTAPPSGYPYSATTP- 180

Query: 181 GSGGYPYGQPAYAPPSYGQPAYAPPSYGQPAYGQPGYAQPSYGQPGGGGYGYGQVAEEKK 240
                PYGQP Y   SYGQP+                          GGYGY    EE+K
Sbjct: 181 -----PYGQPVYGEGSYGQPS--------------------------GGYGY----EEEK 240

Query: 241 KGGKFGGMGTGLAVGAVAGALGGLALVEGFDKLEDHIADEAAEKVEDDLADGGYDDGDYG 300
           K  KFGGMGTGLAVGAVAG LGG+ALVEG D LED IAD+ AEKVEDDLAD   DDGDYG
Sbjct: 241 KKSKFGGMGTGLAVGAVAGVLGGVALVEGVDLLEDKIADDVAEKVEDDLAD---DDGDYG 264

[Close]

BLAST of Spo04457.1 vs. NCBI nr
Match: gi|697098667|ref|XP_009627866.1| (PREDICTED: uncharacterized protein LOC104118341 [Nicotiana tomentosiformis])

HSP 1 Score: 333.2 bits (853), Expect = 4.800e-88
Identity = 188/304 (61.84%), Postives = 218/304 (71.71%), Query Frame = 1


		  
Query: 1   MASRFEVEVTLSSAKDLKNVNWRHGPIKPYAVMWFDPARKITSRVDEEGDTTPHWNQTLV 60
           M SR+EVEV ++SA DLKNVNWR+GP+KPYAV+W DP  K +++VDE+GDT+P+WN+ LV
Sbjct: 1   MGSRYEVEVKITSANDLKNVNWRYGPLKPYAVVWVDPNAKCSTKVDEDGDTSPYWNEKLV 60

Query: 61  IPLDRPIEDATLHIDIVHAYADEDTKPLIGSAKLPLREVVDDVGVGGRLNRSLKLKRPSG 120
           +PL+ PI+D+TL+IDIVHA A EDTKPLIGSAKLPL +VVDD+G+GG L  +L+LKRPSG
Sbjct: 61  VPLNSPIDDSTLYIDIVHANAVEDTKPLIGSAKLPLSKVVDDIGIGGELECTLQLKRPSG 120

Query: 121 RPHGKVEVEVVVR-QPRYHAPDPYGAPPYGVASKDAGYG---GAPYMAPPTGYPHMAPPP 180
           RP GKV+VEV VR QPRY AP+ Y APPYGV      YG   GAPY APPTGYP+ A  P
Sbjct: 121 RPQGKVQVEVSVRDQPRYRAPEAYYAPPYGVPPPSQPYGRDYGAPYTAPPTGYPYSATTP 180

Query: 181 GGSGGYPYGQPAYAPPSYGQPAYAPPSYGQPAYGQPGYAQPSYGQPGGGGYGYGQVAEEK 240
                 PYGQPAY   SYGQ +                          GGYGY    EE+
Sbjct: 181 ------PYGQPAYGEGSYGQLS--------------------------GGYGY----EEE 240

Query: 241 KKGGKFGGMGTGLAVGAVAGALGGLALVEGFDKLEDHIADEAAEKVEDDLADGGYDDGDY 300
           KK  KFGGMGTGLAVGAVAG LGG+AL EG D LED IAD+ AEKVEDDLAD   DDGDY
Sbjct: 241 KKKSKFGGMGTGLAVGAVAGVLGGVALAEGVDLLEDKIADDVAEKVEDDLAD---DDGDY 265

[Close]

BLAST of Spo04457.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RXU7_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_016910 PE=4 SV=1)

HSP 1 Score: 606.3 bits (1562), Expect = 2.100e-170
Identity = 303/304 (99.67%), Postives = 303/304 (99.67%), Query Frame = 1


		  
Query: 1   MASRFEVEVTLSSAKDLKNVNWRHGPIKPYAVMWFDPARKITSRVDEEGDTTPHWNQTLV 60
           MASRFEVEVTLSSAKDLKNVNWRHGPIKPYAVMWFDPARKITSRVDEEGDTTPHWNQTLV
Sbjct: 1   MASRFEVEVTLSSAKDLKNVNWRHGPIKPYAVMWFDPARKITSRVDEEGDTTPHWNQTLV 60

Query: 61  IPLDRPIEDATLHIDIVHAYADEDTKPLIGSAKLPLREVVDDVGVGGRLNRSLKLKRPSG 120
           IPLDRPIEDATLHIDIVHAYADEDTKPLIGSAKLPLREVVDDVGVGGRLNRSLKLKRPSG
Sbjct: 61  IPLDRPIEDATLHIDIVHAYADEDTKPLIGSAKLPLREVVDDVGVGGRLNRSLKLKRPSG 120

Query: 121 RPHGKVEVEVVVRQPRYHAPDPYGAPPYGVASKDAGYGGAPYMAPPTGYPHMAPPPGGSG 180
           RPHGKVEVEVVVRQPRYH PDPYGAPPYGVASKDAGYGGAPYMAPPTGYPHMAPPPGGSG
Sbjct: 121 RPHGKVEVEVVVRQPRYHTPDPYGAPPYGVASKDAGYGGAPYMAPPTGYPHMAPPPGGSG 180

Query: 181 GYPYGQPAYAPPSYGQPAYAPPSYGQPAYGQPGYAQPSYGQPGGGGYGYGQVAEEKKKGG 240
           GYPYGQPAYAPPSYGQPAYAPPSYGQPAYGQPGYAQPSYGQPGGGGYGYGQVAEEKKKGG
Sbjct: 181 GYPYGQPAYAPPSYGQPAYAPPSYGQPAYGQPGYAQPSYGQPGGGGYGYGQVAEEKKKGG 240

Query: 241 KFGGMGTGLAVGAVAGALGGLALVEGFDKLEDHIADEAAEKVEDDLADGGYDDGDYGGGD 300
           KFGGMGTGLAVGAVAGALGGLALVEGFDKLEDHIADEAAEKVEDDLADGGYDDGDYGGGD
Sbjct: 241 KFGGMGTGLAVGAVAGALGGLALVEGFDKLEDHIADEAAEKVEDDLADGGYDDGDYGGGD 300

Query: 301 DDGW 305
           DDGW
Sbjct: 301 DDGW 304

[Close]

BLAST of Spo04457.1 vs. UniProtKB/TrEMBL
Match: A0A068VMH9_COFCA (Uncharacterized protein OS=Coffea canephora GN=GSCOC_T00005975001 PE=4 SV=1)

HSP 1 Score: 337.0 bits (863), Expect = 2.300e-89
Identity = 193/308 (62.66%), Postives = 224/308 (72.73%), Query Frame = 1


		  
Query: 1   MASRFEVEVTLSSAKDLKNVNWRHGPIKPYAVMWFDPARKITSRVDEEGDTTPHWNQTLV 60
           M SR+EVEV ++SAKDLKNVNWRHGP+KPYAV+W DP  K ++RVD+EGDT+P+W +TLV
Sbjct: 1   MGSRYEVEVAITSAKDLKNVNWRHGPLKPYAVVWVDPKAKCSTRVDDEGDTSPYWEETLV 60

Query: 61  IPLDRPIEDATLHIDIVHAYADEDTKPLIGSAKLPLREVVDDVGVGGRLNRSLKLKRPSG 120
           IP D PIED+TL ID+VHA A EDTKPLIGSA+L L +VVD+ G+G R+ R L+LKRPSG
Sbjct: 61  IPFDSPIEDSTLLIDVVHAKAAEDTKPLIGSARLRLIDVVDEAGLGRRVERKLELKRPSG 120

Query: 121 RPHGKVEVEVVVRQ-PRYHAPDPYGAPPYGV---ASKDAG----YGGAPYMAPPTGYPHM 180
           RPHGK++V+V VR+ PRY APDPY APPYGV    S+D      YG  PY APP    + 
Sbjct: 121 RPHGKLQVQVSVREPPRYRAPDPYYAPPYGVPQPGSRDYAAPQPYGNPPYGAPPAS-AYA 180

Query: 181 APPPGGSGGYPYGQPAYAPPSYGQPAYAPPSYGQPAYGQPGYAQPSYGQPGGGGYGYGQV 240
           APP     GYPY  P         PA  PPSYGQP+YGQP Y QPSYGQP         V
Sbjct: 181 APP----AGYPYSAP---------PAAPPPSYGQPSYGQPAYGQPSYGQP---------V 240

Query: 241 AEEKKKGGKFGGMGTGLAVGAVAGALGGLALVEGFDKLEDHIADEAAEKVEDDLADGGYD 300
             E+KK  KFGGMGTGLAVGAVAGALGGLAL EG D  +D IAD+AAEKVE+D+   GYD
Sbjct: 241 YGEEKKKSKFGGMGTGLAVGAVAGALGGLALFEGIDYAKDKIADDAAEKVEEDI---GYD 280

[Close]

BLAST of Spo04457.1 vs. UniProtKB/TrEMBL
Match: A0A059DJS1_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_A02756 PE=4 SV=1)

HSP 1 Score: 318.5 bits (815), Expect = 8.600e-84
Identity = 190/303 (62.71%), Postives = 220/303 (72.61%), Query Frame = 1


		  
Query: 1   MASRFEVEVTLSSAKDLKNVNWRHGPIKPYAVMWFDPARKITSRVDEEGDTTPHWNQTLV 60
           MASR+E+EVT+SSAKDLKNVNWR GP +PYAV+W DP++K ++RVDEE DT+P W+QTLV
Sbjct: 1   MASRYELEVTVSSAKDLKNVNWRQGPTRPYAVLWVDPSKKCSTRVDEENDTSPVWDQTLV 60

Query: 61  IPLDR-PIEDATLHIDIVHAYADEDTKPLIGSAKLPLREVVDDVGVGGRLNRSLKLKRPS 120
           IPL   PIEDATL+ID+VHA ++EDTKPLIGSA+L LR+V+DD G G  L R L+LKRPS
Sbjct: 61  IPLPPGPIEDATLYIDVVHAGSEEDTKPLIGSARLKLRDVLDDAGFGEALKRGLQLKRPS 120

Query: 121 GRPHGKVEVEVVVRQPRYHAPDPYGAPPYGVASK------DAGYGGAPYMAPPTGYPHMA 180
           GRPHGKVEV+V VR+PRY APDPY A PYG A           YG  PY AP     + A
Sbjct: 121 GRPHGKVEVKVCVREPRYRAPDPYHASPYGYAPPATREYASPAYGN-PYAAPQRDPYYAA 180

Query: 181 PPPGGSGGYPYGQPAYAPPSYGQPAYAPPSYGQ-PAYGQPGYAQPSYGQPGGGGYGYGQV 240
            PP  +GGY YG     PP   QP Y  PSYGQ P YG  G  +P+YGQ       YGQ 
Sbjct: 181 APP--AGGYAYGAYNAPPP---QPQYGQPSYGQAPQYGYGG--EPAYGQ-------YGQP 240

Query: 241 AEEKKKGGKFG--GMGTGLAVGAVAGALGGLALVEGFDKLEDHIADEAAEKVEDDLADGG 294
            E+KK   KFG  GMGTGLAVGAVAGA+GG+AL EGF+ LED IAD+AAEKVEDDL   G
Sbjct: 241 EEKKKS--KFGGMGMGTGLAVGAVAGAIGGIALTEGFEHLEDKIADDAAEKVEDDL---G 283

[Close]

BLAST of Spo04457.1 vs. UniProtKB/TrEMBL
Match: A0A0V0HFV8_SOLCH (Putative ovule protein OS=Solanum chacoense PE=4 SV=1)

HSP 1 Score: 315.1 bits (806), Expect = 9.500e-83
Identity = 187/306 (61.11%), Postives = 221/306 (72.22%), Query Frame = 1


		  
Query: 1   MASRFEVEVTLSSAKDLKNVNWRHGPIKPYAVMWFDPARKITSRVDEEGDTTPHWNQTLV 60
           M SR+EVEV ++SAKDLKN+NWR+GP+KPYAV+W DP  K +++VDE+GDT+P+WN  L+
Sbjct: 1   MGSRYEVEVKITSAKDLKNINWRYGPLKPYAVVWVDPNSKCSTKVDEDGDTSPYWNDKLL 60

Query: 61  IPLDRPIEDATLHIDIVHAY-ADEDTKPLIGSAKLPLREVVDDVGVGGRLNRSLKLKRPS 120
           IPL+ PI+++ L+IDIVHA  A+EDTKPLIGSAK+ L EVVD+ GVGG + R+L+LKRPS
Sbjct: 61  IPLNSPIDESVLYIDIVHAVGAEEDTKPLIGSAKISLSEVVDEAGVGGEVERTLQLKRPS 120

Query: 121 GRPHGKVEVEVVVRQPRYHAPDPYGAPPYGVASKDAGYGGAPYMAPPTGYPHMAPPPGGS 180
           GRP GKVEV+V VR PRY A D Y APPYGV              PP   P+       +
Sbjct: 121 GRPQGKVEVQVTVRDPRYRARDAYYAPPYGV--------------PPPAQPY-------N 180

Query: 181 GGYPYGQPAYAPPSYGQPAYAPPSYGQPAYGQPGYAQP---SYGQPGGGGYGYGQVAEEK 240
            G  YG P    P YGQP  AP SYGQP YG  GY QP   SYGQP GGGYGY    EE+
Sbjct: 181 YGGXYGAPI---PPYGQP--APGSYGQPGYGMGGYGQPAVGSYGQP-GGGYGY----EEE 240

Query: 241 KKGGKFGGMGTGLAVGAVAGALGGLALVEGFDKLEDHIADEAAEKVEDDLADGGYDDGDY 300
           KK  KF GMGTGLAVGAVAG LGG+A+ EG D LED IA++AAEKVE+DLAD   DDGDY
Sbjct: 241 KKKSKF-GMGTGLAVGAVAGVLGGVAIAEGIDHLEDSIAEDAAEKVEEDLAD---DDGDY 269

Query: 301 GGGDDD 303
             GDDD
Sbjct: 301 --GDDD 269

[Close]

BLAST of Spo04457.1 vs. UniProtKB/TrEMBL
Match: M1CXL8_SOLTU (Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG400029920 PE=4 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 2.100e-82
Identity = 187/305 (61.31%), Postives = 223/305 (73.11%), Query Frame = 1


		  
Query: 1   MASRFEVEVTLSSAKDLKNVNWRHGPIKPYAVMWFDPARKITSRVDEEGDTTPHWNQTLV 60
           M SR+EVEV ++SAKDLKN+NWR+GP+KPYAV+W DP  K +++VDE+GDT+P+W   L+
Sbjct: 1   MGSRYEVEVKITSAKDLKNINWRYGPLKPYAVVWVDPNSKCSTKVDEDGDTSPYWTDKLL 60

Query: 61  IPLDRPIEDATLHIDIVHAY-ADEDTKPLIGSAKLPLREVVDDVGVGGRLNRSLKLKRPS 120
           IPL+ PI+++ L+IDIVHA  A+EDTKPLIGSAK+ L EVVD+ GVGG + R+L+LKRPS
Sbjct: 61  IPLNSPIDESVLYIDIVHAVGAEEDTKPLIGSAKISLSEVVDEAGVGGEVERTLQLKRPS 120

Query: 121 GRPHGKVEVEVVVRQPRYHAPDPYGAPPYGVA--SKDAGYGGAPYMAPPTGYPHMAPPPG 180
           GRP GKVEV+V VR PRY A D Y APPYGV   ++   YGGA          + AP P 
Sbjct: 121 GRPQGKVEVQVTVRDPRYRARDAYYAPPYGVPPPAQPYNYGGA----------YGAPIP- 180

Query: 181 GSGGYPYGQPAYAPPSYGQPAYAPPSYGQPAYGQPGYAQPSYGQPGGGGYGYGQVAEEKK 240
                PYGQPA  P SYGQPAY    YGQPA G       SYGQPGGG YGY    EE+K
Sbjct: 181 -----PYGQPA--PGSYGQPAYGMGGYGQPAVG-------SYGQPGGG-YGY----EEEK 240

Query: 241 KGGKFGGMGTGLAVGAVAGALGGLALVEGFDKLEDHIADEAAEKVEDDLADGGYDDGDYG 300
           K  KFG +GTGLAVGAVAG LGG+A+ EG D LED IA++AAEKVE+DLAD   DDGDY 
Sbjct: 241 KKSKFG-LGTGLAVGAVAGVLGGVAIAEGIDHLEDSIAEDAAEKVEEDLAD---DDGDY- 269

Query: 301 GGDDD 303
            GDDD
Sbjct: 301 -GDDD 269

[Close]

BLAST of Spo04457.1 vs. TAIR (Arabidopsis)
Match: AT5G23950.1 (Calcium-dependent lipid-binding (CaLB domain) family protein)

HSP 1 Score: 163.7 bits (413), Expect = 1.800e-40
Identity = 87/156 (55.77%), Postives = 112/156 (71.79%), Query Frame = 1


		  
Query: 6   EVEVTLSSAKDLKNVNWRHGPIKPYAVMWFDPARKITSRVDEEGDTTPHWNQTLVIPL-D 65
           EVEVT+SSAKD+KNVNWR+GP KPYAV+W DP  K ++RVDE+G+T   WN+T VI L  
Sbjct: 7   EVEVTISSAKDIKNVNWRNGPNKPYAVVWIDPKFKSSTRVDEDGNTCTTWNETFVIALPP 66

Query: 66  RPIEDATLHIDIVHAYADEDTKPLIGSAKLPLREVVDDVGVGGRLNRSLKLKRPSGRPHG 125
              +D  ++I+IVHA  +E+TKPLIGSA L LR+V+DDVG G    ++LKLKRPSGRP G
Sbjct: 67  ANDDDDKVYINIVHAGREENTKPLIGSAHLSLRDVIDDVGFGVPFMKTLKLKRPSGRPQG 126

Query: 126 KVEVEVVVRQ-PRYHAPDPYGAPPYGVASKDAGYGG 160
           K++V V VR+ P  +   PYG P      K + +GG
Sbjct: 127 KLDVTVTVRETPGSNYALPYGDP--YAPEKGSKFGG 160

[Close]

BLAST of Spo04457.1 vs. TAIR (Arabidopsis)
Match: AT1G07310.1 (Calcium-dependent lipid-binding (CaLB domain) family protein)

HSP 1 Score: 105.1 bits (261), Expect = 7.500e-23
Identity = 71/227 (31.28%), Postives = 116/227 (51.10%), Query Frame = 1


		  
Query: 6   EVEVTLSSAKDLKNVNWRHGPIKPYAVMWFDPARKITSRVDEEGDTTPHWNQTLVIPLDR 65
           ++ VT+ SAK LKNVNWR+G +KPY V++ D    +++R D+     P WN+ + +PL R
Sbjct: 8   DLVVTVVSAKHLKNVNWRNGDLKPYVVLYLDQDHPLSTRSDDSSSIKPVWNERITLPLTR 67

Query: 66  PIEDATLHIDIVHAYADEDTKPLIGSAKLPLREVVD-DVGVGGRLNRSLKLKRPSGRPHG 125
            + ++ L+I++ H+ + +  K L+GS + PL  ++D D  +   L  SL+L RPSGRP G
Sbjct: 68  SVHESVLNIEVFHSNSSDLAKTLVGSVRFPLARLIDSDGSMIPELINSLELVRPSGRPQG 127

Query: 126 KVEVEVVVRQ-----PRYHAPDPYGAP--PYGVASKDAGYGGAPYMAPPTGYPHMAPPPG 185
           K+ +++ ++      P++  P P   P   Y     +  Y  +P   PP   P  AP P 
Sbjct: 128 KIRLKLAIKDRPIPPPQHPPPRPQSQPLDYYSAPQGNHYYSPSPPPPPPPQAPITAPSPQ 187

Query: 186 GSGGYPYGQPAYAPPSYGQPAYAPPSYGQPAYGQPGYAQPSYGQPGG 225
                    P+ +P ++    Y+   Y  P          +YG P G
Sbjct: 188 RDYREFSQSPSPSPYAFTDHYYSGYYYPPPPPRSMYDRASNYGLPSG 234

[Close]

BLAST of Spo04457.1 vs. TAIR (Arabidopsis)
Match: AT2G13350.1 (Calcium-dependent lipid-binding (CaLB domain) family protein)

HSP 1 Score: 66.2 bits (160), Expect = 3.900e-11
Identity = 43/126 (34.13%), Postives = 69/126 (54.76%), Query Frame = 1


		  
Query: 7   VEVTLSSAKDLKNVNWRHGPIKPYAVMWFDPARKITSRVDEEGDTTPHWNQTLVIPLD-R 66
           +E+ + SA++L  V      +K YA+ W DP RK+T+RVD  G T+P WN   V  LD  
Sbjct: 10  LELNIISAQELAPVA---RCMKTYAIAWIDPERKLTTRVDNTGGTSPTWNDKFVFRLDEE 69

Query: 67  PIEDATLHIDIVHAYADEDTKPL-IGSAKLPLREVVDDVGVGGRLNRSLKLKRPSGRPHG 126
            + DAT  I ++  YA    K + +G+ +  + ++V        +  +L++ R SGRPHG
Sbjct: 70  ALYDAT-SIVVIEIYALHWFKDIHVGTVQALISDLVSPSSAMRFV--TLEVLRASGRPHG 129

Query: 127 KVEVEV 131
            + + V
Sbjct: 130 LLNIAV 129

[Close]

BLAST of Spo04457.1 vs. TAIR (Arabidopsis)
Match: AT3G04360.1 (Calcium-dependent lipid-binding (CaLB domain) family protein)

HSP 1 Score: 58.5 bits (140), Expect = 8.100e-9
Identity = 44/144 (30.56%), Postives = 72/144 (50.00%), Query Frame = 1


		  
Query: 7   VEVTLSSAKDLKNVNWRHGPIKPYAVMWF--DPARKITSRVDEEGDTTPHWNQTLVIPLD 66
           +E+ L SA+DL  V+     +K Y+V W   DP RK+T+RVD+     P WN+  V    
Sbjct: 11  LEINLISAQDLAPVSRN---MKTYSVAWINTDPMRKLTTRVDQSNRANPIWNEKFVF--- 70

Query: 67  RPIEDATLHID----IVHAYADEDTK-PLIGSAKLPLREVV--------DDVGVGGRLNR 126
             + D  L++D    ++  YA    K  L+G+  + L ++          + G GG  N 
Sbjct: 71  -RVNDKILYVDASAIVIEIYAAAWAKDALVGTVNVLLSDLFAPWSGFGDGNDGGGGNNNM 130

Query: 127 ---SLKLKRPSGRPHGKVEVEVVV 133
              +L+++RPSGR  G + + V +
Sbjct: 131 RLVTLQIRRPSGRLQGFLRLGVAL 147

[Close]

BLAST of Spo04457.1 vs. TAIR (Arabidopsis)
Match: AT1G04540.1 (Calcium-dependent lipid-binding (CaLB domain) family protein)

HSP 1 Score: 50.1 bits (118), Expect = 2.900e-6
Identity = 37/140 (26.43%), Postives = 65/140 (46.43%), Query Frame = 1


		  
Query: 7   VEVTLSSAKDLKNVNWRHGPIKPYAVMWFDPARKITSRVDEEGDTTPHWNQTLVIPLDRP 66
           +E+ + SA+DL  V  +    K YAV W    RK+T+RVD  G T P WN   V  ++  
Sbjct: 11  LELNIISAQDLAPVARK---TKTYAVAWVHSERKLTTRVDYNGGTNPTWNDKFVFRVNEE 70

Query: 67  IEDATLHIDIVHAYADEDTKPL-IGSAKLPLREVV-DDVGVGGRLNR------------- 126
              A     ++  YA    + + +G+ ++ +  ++  +   G R +              
Sbjct: 71  FLYADTSAVVIEIYALHWFRDVHVGTVRVLISNLIPPNRRPGYRTSNNEYRRTPPPGMRF 130

Query: 127 -SLKLKRPSGRPHGKVEVEV 131
            +L+++R SGRP G + + V
Sbjct: 131 VALQVRRTSGRPQGILNIGV 147

The following BLAST results are available for this feature:

BLAST of Spo04457.1 vs. NCBI nr
BLAST of Spo04457.1 vs. UniProtKB/TrEMBL
BLAST of Spo04457.1 vs. ExPASy Swiss-Prot
BLAST of Spo04457.1 vs. TAIR (Arabidopsis)

BLAST of Spo04457.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902236244\|gb\|KNA24283.1\|	2.9e-170	99.6	hypothetical protein SOVF_0169...	[more]
gi\|747059492\|ref\|XP_011076131.1\|	3.0e-90	65.1	PREDICTED: WW domain-containin...	[more]
gi\|661867246\|emb\|CDP21817.1\|	3.3e-89	62.6	unnamed protein product [Coffe...	[more]
gi\|698571473\|ref\|XP_009774894.1\|	3.7e-88	60.7	PREDICTED: uncharacterized pro...	[more]
gi\|697098667\|ref\|XP_009627866.1\|	4.8e-88	61.8	PREDICTED: uncharacterized pro...	[more]

BLAST of Spo04457.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9RXU7_SPIOL	2.1e-170	99.6	Uncharacterized protein OS=Spi...	[more]
A0A068VMH9_COFCA	2.3e-89	62.6	Uncharacterized protein OS=Cof...	[more]
A0A059DJS1_EUCGR	8.6e-84	62.7	Uncharacterized protein OS=Euc...	[more]
A0A0V0HFV8_SOLCH	9.5e-83	61.1	Putative ovule protein OS=Sola...	[more]
M1CXL8_SOLTU	2.1e-82	61.3	Uncharacterized protein OS=Sol...	[more]

BLAST of Spo04457.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Spo04457.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 5


Match Name	E-value	Identity	Description
AT5G23950.1	1.8e-40	55.7	Calcium-dependent lipid-bindin...	[more]
AT1G07310.1	7.5e-23	31.2	Calcium-dependent lipid-bindin...	[more]
AT2G13350.1	3.9e-11	34.1	Calcium-dependent lipid-bindin...	[more]
AT3G04360.1	8.1e-9	30.5	Calcium-dependent lipid-bindin...	[more]
AT1G04540.1	2.9e-6	26.4	Calcium-dependent lipid-bindin...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000008	C2 domain	GENE3D	2.60.40.150		coord: 4..130 score: 4.7
IPR000008	C2 domain	PFAM	PF00168	C2	coord: 7..100 score: 1.
IPR000008	C2 domain	SMART	SM00239	C2_3c	coord: 6..108 score: 4.
IPR000008	C2 domain	unknown	SSF49562	C2 domain (Calcium/lipid-binding domain, CaLB)	coord: 4..132 score: 2.65
None	No IPR available	PANTHER	PTHR32246	FAMILY NOT NAMED	coord: 1..302 score: 5.2E
None	No IPR available	PANTHER	PTHR32246:SF17	SUBFAMILY NOT NAMED	coord: 1..302 score: 5.2E

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005515	protein binding