Spo04872.1 (mRNA) | SpinachBase

Overview


Name	Spo04872.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	Probable calcium-binding protein CML36 (Calmodulin-like protein 36)
Location	SpoScf_01894 : 3958 .. 4611 (+)
Sequence length	654

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGCAACTCTTGAAATCCATCAACCCAAAACAAATTTTCCGCCTTAAGAAGCCAAAGTCATCTTCCACCATTACCAAATCCGAGCCACCGTCTTTCGGTTCTTCGTCTTACTCCTCCACCTCGTCCTCCGATTCTCCTTCCTCCGCTACCCATCACCACCGCTCTGGTCCAGCCCCGGCTAGAACCCAATCGGAGGATATCTCCCTTCGGTCCGACGCCTGGACTGAGGAAGCTTTCAAGATGATCGACCTTGACGGGGACGGGAAAATCACTCGCGTAGAACTACACTCACTTTTCAAGCGCGTAAGAGTAAACGCGCCGTCGTTGAGTGAGGAGGAGGTTGCTGCGATGATCAAAGAAATTGATATCGACGGAGACGGTTGTATCAGCTTAAAAGAGCTGGAGGCCGTTGGATCTGCGCTTGATCCAACGTCTGCCAATGAAGAAGATCTTAAGGGCGCGTTTGATTTCTTTGATACAGATCATGATGGTAAAATATCAGCGAGTGAGCTGCACTCGGGGTTTTTTGCGTTGGGAGATGAACGGTGCACGTTAGATGACTGCCGGCGCATGATAGAAGGTGTGGATAAGACTAAAGATGGGTTTGTTTGTTTTGAGGATTTTTGCCGTATGATGAGCTTGCCTAGATAA

mRNA sequence

ATGAAGCAACTCTTGAAATCCATCAACCCAAAACAAATTTTCCGCCTTAAGAAGCCAAAGTCATCTTCCACCATTACCAAATCCGAGCCACCGTCTTTCGGTTCTTCGTCTTACTCCTCCACCTCGTCCTCCGATTCTCCTTCCTCCGCTACCCATCACCACCGCTCTGGTCCAGCCCCGGCTAGAACCCAATCGGAGGATATCTCCCTTCGGTCCGACGCCTGGACTGAGGAAGCTTTCAAGATGATCGACCTTGACGGGGACGGGAAAATCACTCGCGTAGAACTACACTCACTTTTCAAGCGCGTAAGAGTAAACGCGCCGTCGTTGAGTGAGGAGGAGGTTGCTGCGATGATCAAAGAAATTGATATCGACGGAGACGGTTGTATCAGCTTAAAAGAGCTGGAGGCCGTTGGATCTGCGCTTGATCCAACGTCTGCCAATGAAGAAGATCTTAAGGGCGCGTTTGATTTCTTTGATACAGATCATGATGGTAAAATATCAGCGAGTGAGCTGCACTCGGGGTTTTTTGCGTTGGGAGATGAACGGTGCACGTTAGATGACTGCCGGCGCATGATAGAAGGTGTGGATAAGACTAAAGATGGGTTTGTTTGTTTTGAGGATTTTTGCCGTATGATGAGCTTGCCTAGATAA

Coding sequence (CDS)

ATGAAGCAACTCTTGAAATCCATCAACCCAAAACAAATTTTCCGCCTTAAGAAGCCAAAGTCATCTTCCACCATTACCAAATCCGAGCCACCGTCTTTCGGTTCTTCGTCTTACTCCTCCACCTCGTCCTCCGATTCTCCTTCCTCCGCTACCCATCACCACCGCTCTGGTCCAGCCCCGGCTAGAACCCAATCGGAGGATATCTCCCTTCGGTCCGACGCCTGGACTGAGGAAGCTTTCAAGATGATCGACCTTGACGGGGACGGGAAAATCACTCGCGTAGAACTACACTCACTTTTCAAGCGCGTAAGAGTAAACGCGCCGTCGTTGAGTGAGGAGGAGGTTGCTGCGATGATCAAAGAAATTGATATCGACGGAGACGGTTGTATCAGCTTAAAAGAGCTGGAGGCCGTTGGATCTGCGCTTGATCCAACGTCTGCCAATGAAGAAGATCTTAAGGGCGCGTTTGATTTCTTTGATACAGATCATGATGGTAAAATATCAGCGAGTGAGCTGCACTCGGGGTTTTTTGCGTTGGGAGATGAACGGTGCACGTTAGATGACTGCCGGCGCATGATAGAAGGTGTGGATAAGACTAAAGATGGGTTTGTTTGTTTTGAGGATTTTTGCCGTATGATGAGCTTGCCTAGATAA

Protein sequence

MKQLLKSINPKQIFRLKKPKSSSTITKSEPPSFGSSSYSSTSSSDSPSSATHHHRSGPAPARTQSEDISLRSDAWTEEAFKMIDLDGDGKITRVELHSLFKRVRVNAPSLSEEEVAAMIKEIDIDGDGCISLKELEAVGSALDPTSANEEDLKGAFDFFDTDHDGKISASELHSGFFALGDERCTLDDCRRMIEGVDKTKDGFVCFEDFCRMMSLPR

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo04872	Spo04872	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo04872.1	Spo04872.1-protein	polypeptide

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo04872.1.exon.1	Spo04872.1.exon.1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo04872.1.CDS.1	Spo04872.1.CDS.1	CDS

Homology

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BLAST of Spo04872.1 vs. NCBI nr
Match: gi|902226899|gb|KNA20519.1| (hypothetical protein SOVF_051520 [Spinacia oleracea])

HSP 1 Score: 421.0 bits (1081), Expect = 1.300e-114
Identity = 217/217 (100.00%), Postives = 217/217 (100.00%), Query Frame = 1


		  
Query: 1   MKQLLKSINPKQIFRLKKPKSSSTITKSEPPSFGSSSYSSTSSSDSPSSATHHHRSGPAP 60
           MKQLLKSINPKQIFRLKKPKSSSTITKSEPPSFGSSSYSSTSSSDSPSSATHHHRSGPAP
Sbjct: 1   MKQLLKSINPKQIFRLKKPKSSSTITKSEPPSFGSSSYSSTSSSDSPSSATHHHRSGPAP 60

Query: 61  ARTQSEDISLRSDAWTEEAFKMIDLDGDGKITRVELHSLFKRVRVNAPSLSEEEVAAMIK 120
           ARTQSEDISLRSDAWTEEAFKMIDLDGDGKITRVELHSLFKRVRVNAPSLSEEEVAAMIK
Sbjct: 61  ARTQSEDISLRSDAWTEEAFKMIDLDGDGKITRVELHSLFKRVRVNAPSLSEEEVAAMIK 120

Query: 121 EIDIDGDGCISLKELEAVGSALDPTSANEEDLKGAFDFFDTDHDGKISASELHSGFFALG 180
           EIDIDGDGCISLKELEAVGSALDPTSANEEDLKGAFDFFDTDHDGKISASELHSGFFALG
Sbjct: 121 EIDIDGDGCISLKELEAVGSALDPTSANEEDLKGAFDFFDTDHDGKISASELHSGFFALG 180

Query: 181 DERCTLDDCRRMIEGVDKTKDGFVCFEDFCRMMSLPR 218
           DERCTLDDCRRMIEGVDKTKDGFVCFEDFCRMMSLPR
Sbjct: 181 DERCTLDDCRRMIEGVDKTKDGFVCFEDFCRMMSLPR 217

[Close]

BLAST of Spo04872.1 vs. NCBI nr
Match: gi|731324427|ref|XP_010672963.1| (PREDICTED: probable calcium-binding protein CML35 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 306.2 bits (783), Expect = 4.500e-80
Identity = 163/220 (74.09%), Postives = 182/220 (82.73%), Query Frame = 1


		  
Query: 1   MKQLLKSINPKQIFR---LKKPKSSSTITKSEPPSFGSSSYSSTSSSDSPSSATHHHRSG 60
           MKQLLKS++PKQIFR    K P SS ++TKSEPPSFGSSS SS+S+S           SG
Sbjct: 1   MKQLLKSLHPKQIFRHNNKKTPLSSHSLTKSEPPSFGSSSSSSSSAS-----------SG 60

Query: 61  PAPARTQSEDISLRSDAWTEEAFKMIDLDGDGKITRVELHSLFKRVRVNAPSLSEEEVAA 120
           P P RT+SED+S+R D WTEEAFKM+DLDGDGKITRVELHSLFKRVRVNAPSLSEEEVA 
Sbjct: 61  PGPGRTRSEDLSVRPDPWTEEAFKMMDLDGDGKITRVELHSLFKRVRVNAPSLSEEEVAE 120

Query: 121 MIKEIDIDGDGCISLKELEAVGSALDPTSANEEDLKGAFDFFDTDHDGKISASELHSGFF 180
           MIK+ID DGDGCISL+ELEAVGSALDPT A+E+DLK AF FFD + DGKIS  ELH+GF 
Sbjct: 121 MIKDIDTDGDGCISLEELEAVGSALDPTGADEDDLKDAFGFFDVNKDGKISPEELHAGFV 180

Query: 181 ALGDERCTLDDCRRMIEGVDKTKDGFVCFEDFCRMMSLPR 218
           ALGDERCTL++CRRMIEGVDK KDGFVCF+DF RMM   R
Sbjct: 181 ALGDERCTLEECRRMIEGVDKNKDGFVCFQDFSRMMRFSR 209

[Close]

BLAST of Spo04872.1 vs. NCBI nr
Match: gi|225441325|ref|XP_002276334.1| (PREDICTED: probable calcium-binding protein CML35 [Vitis vinifera])

HSP 1 Score: 183.0 bits (463), Expect = 5.700e-43
Identity = 117/224 (52.23%), Postives = 144/224 (64.29%), Query Frame = 1


		  
Query: 3   QLLKSINPKQIFRLKKPKSSSTITKSEPPSFGSSSYSSTSSSDSPSSATHHHRSG----- 62
           +L+  INPKQ+FR KK +S   +T+++P SF S + SSTSS  S +  T     G     
Sbjct: 2   KLIYKINPKQLFRSKKSRS---VTRTDPSSFSSGTSSSTSSDSSTNLKTGTGACGGGMGT 61

Query: 63  -----PAPARTQSEDIS-LRSDAWTE--EAFKMIDLDGDGKITRVELHSLFKRVRVNAPS 122
                P  +   S D S   SD  TE   AFKMID DGDGKIT+ EL +L  RV V  PS
Sbjct: 62  PTSVLPMHSYEISGDWSDFSSDIQTELVHAFKMIDRDGDGKITKRELEALLSRVGVEPPS 121

Query: 123 LSEEEVAAMIKEIDIDGDGCISLKELEAVGSALDPTSANEEDLKGAFDFFDTDHDGKISA 182
             EEE+  M+ E+D DGDGCISL+E  A+ SA  P  A + +L+ AF FFDTD DGKI+A
Sbjct: 122 --EEEIMMMLSEVDRDGDGCISLEEFGAISSAFGP--ACDTELRDAFCFFDTDRDGKITA 181

Query: 183 SELHSGFFALGDERCTLDDCRRMIEGVDKTKDGFVCFEDFCRMM 214
            EL+  F A+GD+RCTL+DC+RMI GVDK  DGFVCFEDF RMM
Sbjct: 182 EELNQVFAAIGDDRCTLEDCQRMIAGVDKNGDGFVCFEDFSRMM 218

[Close]

BLAST of Spo04872.1 vs. NCBI nr
Match: gi|697172930|ref|XP_009595400.1| (PREDICTED: probable calcium-binding protein CML36 [Nicotiana tomentosiformis])

HSP 1 Score: 181.4 bits (459), Expect = 1.700e-42
Identity = 109/217 (50.23%), Postives = 144/217 (66.36%), Query Frame = 1


		  
Query: 3   QLLKSINPKQIFRLKKPKSSSTITKSEPPSFGSSSYSSTSSSDSPSSATHHHRSGPAPAR 62
           +LLK+I P ++F+ KK +S   I++SE PSFGS + SS S  DS S       +G A   
Sbjct: 2   KLLKNI-PNKLFKSKKSRS---ISRSENPSFGSGTTSSCS--DSESHRGFKKPNGLATPT 61

Query: 63  TQSEDISLRSDAWTE--EAFKMIDLDGDGKITRVELHSLFKRVRVNAPSLSEEEVAAMIK 122
           +      + +D W+E  +AF MID DGDGKI + +L ++  RV  + PS  EEE+ +++ 
Sbjct: 62  SVLPPSEISADEWSEILQAFHMIDSDGDGKIRKEQLEAILTRVGPDPPS--EEELISLLN 121

Query: 123 EIDIDGDGCISLKELEAVGSALDPTSANEEDLKGAFDFFDTDHDGKISASELHSGFFALG 182
           E+D +GDGCISL+E  A+ SA  P + ++E L+ AFDFFD DHDGKI+A EL + F  +G
Sbjct: 122 EVDRNGDGCISLEEFGAISSAFGPPACDDE-LRNAFDFFDADHDGKITAEELFNVFRTIG 181

Query: 183 DERCTLDDCRRMIEGVDKTKDGFVCFEDFCRMMSLPR 218
           D RCTLDDCRRMI GVDK  DGFVCFEDFC MM   R
Sbjct: 182 DGRCTLDDCRRMIRGVDKNGDGFVCFEDFCLMMEQQR 209

[Close]

BLAST of Spo04872.1 vs. NCBI nr
Match: gi|1021035939|gb|KZM93722.1| (hypothetical protein DCAR_016967 [Daucus carota subsp. sativus])

HSP 1 Score: 181.0 bits (458), Expect = 2.200e-42
Identity = 111/210 (52.86%), Postives = 141/210 (67.14%), Query Frame = 1


		  
Query: 5   LKSINPKQIFRLKKPKSSSTITKSEPPSFGSSSYSSTSSSDSPSSATHHHRSGPAPARTQ 64
           L +INPK++F+    K + ++++SE  SFGSS+   TSSSD    +THH +   +   T 
Sbjct: 3   LSNINPKKLFK----KKTRSVSRSEQSSFGSSA---TSSSDD---STHHSKPAGSGTPTS 62

Query: 65  SEDISLRSDAWT-EEAFKMIDLDGDGKITRVELHSLFKRVRVNAPSLSEEEVAAMIKEID 124
                  SD     +AFK+ID DGDGKITR EL SL  RV   +  LSEEE+  M++E+D
Sbjct: 63  VLPDPTSSDFCDLVQAFKIIDADGDGKITRAELGSLLGRV--GSDPLSEEELTMMLRELD 122

Query: 125 IDGDGCISLKELEAVGSALDPTSANEEDLKGAFDFFDTDHDGKISASELHSGFFALGDER 184
            DGDGCISL+E  A+ SA  P S + E L+ AFDFFDTDHDGKISA EL   F ++GD  
Sbjct: 123 SDGDGCISLEEFGAISSAFGPPSGDVE-LREAFDFFDTDHDGKISAEELLCVFTSIGDGV 182

Query: 185 CTLDDCRRMIEGVDKTKDGFVCFEDFCRMM 214
           C+L+DCRRMI+GVDK  DGFVCFEDF +MM
Sbjct: 183 CSLEDCRRMIKGVDKNMDGFVCFEDFSKMM 199

[Close]

BLAST of Spo04872.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RNM0_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_051520 PE=4 SV=1)

HSP 1 Score: 421.0 bits (1081), Expect = 8.700e-115
Identity = 217/217 (100.00%), Postives = 217/217 (100.00%), Query Frame = 1


		  
Query: 1   MKQLLKSINPKQIFRLKKPKSSSTITKSEPPSFGSSSYSSTSSSDSPSSATHHHRSGPAP 60
           MKQLLKSINPKQIFRLKKPKSSSTITKSEPPSFGSSSYSSTSSSDSPSSATHHHRSGPAP
Sbjct: 1   MKQLLKSINPKQIFRLKKPKSSSTITKSEPPSFGSSSYSSTSSSDSPSSATHHHRSGPAP 60

Query: 61  ARTQSEDISLRSDAWTEEAFKMIDLDGDGKITRVELHSLFKRVRVNAPSLSEEEVAAMIK 120
           ARTQSEDISLRSDAWTEEAFKMIDLDGDGKITRVELHSLFKRVRVNAPSLSEEEVAAMIK
Sbjct: 61  ARTQSEDISLRSDAWTEEAFKMIDLDGDGKITRVELHSLFKRVRVNAPSLSEEEVAAMIK 120

Query: 121 EIDIDGDGCISLKELEAVGSALDPTSANEEDLKGAFDFFDTDHDGKISASELHSGFFALG 180
           EIDIDGDGCISLKELEAVGSALDPTSANEEDLKGAFDFFDTDHDGKISASELHSGFFALG
Sbjct: 121 EIDIDGDGCISLKELEAVGSALDPTSANEEDLKGAFDFFDTDHDGKISASELHSGFFALG 180

Query: 181 DERCTLDDCRRMIEGVDKTKDGFVCFEDFCRMMSLPR 218
           DERCTLDDCRRMIEGVDKTKDGFVCFEDFCRMMSLPR
Sbjct: 181 DERCTLDDCRRMIEGVDKTKDGFVCFEDFCRMMSLPR 217

[Close]

BLAST of Spo04872.1 vs. UniProtKB/TrEMBL
Match: A0A0J8FGX1_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_3g062390 PE=4 SV=1)

HSP 1 Score: 306.2 bits (783), Expect = 3.100e-80
Identity = 163/220 (74.09%), Postives = 182/220 (82.73%), Query Frame = 1


		  
Query: 1   MKQLLKSINPKQIFR---LKKPKSSSTITKSEPPSFGSSSYSSTSSSDSPSSATHHHRSG 60
           MKQLLKS++PKQIFR    K P SS ++TKSEPPSFGSSS SS+S+S           SG
Sbjct: 1   MKQLLKSLHPKQIFRHNNKKTPLSSHSLTKSEPPSFGSSSSSSSSAS-----------SG 60

Query: 61  PAPARTQSEDISLRSDAWTEEAFKMIDLDGDGKITRVELHSLFKRVRVNAPSLSEEEVAA 120
           P P RT+SED+S+R D WTEEAFKM+DLDGDGKITRVELHSLFKRVRVNAPSLSEEEVA 
Sbjct: 61  PGPGRTRSEDLSVRPDPWTEEAFKMMDLDGDGKITRVELHSLFKRVRVNAPSLSEEEVAE 120

Query: 121 MIKEIDIDGDGCISLKELEAVGSALDPTSANEEDLKGAFDFFDTDHDGKISASELHSGFF 180
           MIK+ID DGDGCISL+ELEAVGSALDPT A+E+DLK AF FFD + DGKIS  ELH+GF 
Sbjct: 121 MIKDIDTDGDGCISLEELEAVGSALDPTGADEDDLKDAFGFFDVNKDGKISPEELHAGFV 180

Query: 181 ALGDERCTLDDCRRMIEGVDKTKDGFVCFEDFCRMMSLPR 218
           ALGDERCTL++CRRMIEGVDK KDGFVCF+DF RMM   R
Sbjct: 181 ALGDERCTLEECRRMIEGVDKNKDGFVCFQDFSRMMRFSR 209

[Close]

BLAST of Spo04872.1 vs. UniProtKB/TrEMBL
Match: F6HLP0_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_08s0007g05790 PE=4 SV=1)

HSP 1 Score: 183.0 bits (463), Expect = 4.000e-43
Identity = 117/224 (52.23%), Postives = 144/224 (64.29%), Query Frame = 1


		  
Query: 3   QLLKSINPKQIFRLKKPKSSSTITKSEPPSFGSSSYSSTSSSDSPSSATHHHRSG----- 62
           +L+  INPKQ+FR KK +S   +T+++P SF S + SSTSS  S +  T     G     
Sbjct: 2   KLIYKINPKQLFRSKKSRS---VTRTDPSSFSSGTSSSTSSDSSTNLKTGTGACGGGMGT 61

Query: 63  -----PAPARTQSEDIS-LRSDAWTE--EAFKMIDLDGDGKITRVELHSLFKRVRVNAPS 122
                P  +   S D S   SD  TE   AFKMID DGDGKIT+ EL +L  RV V  PS
Sbjct: 62  PTSVLPMHSYEISGDWSDFSSDIQTELVHAFKMIDRDGDGKITKRELEALLSRVGVEPPS 121

Query: 123 LSEEEVAAMIKEIDIDGDGCISLKELEAVGSALDPTSANEEDLKGAFDFFDTDHDGKISA 182
             EEE+  M+ E+D DGDGCISL+E  A+ SA  P  A + +L+ AF FFDTD DGKI+A
Sbjct: 122 --EEEIMMMLSEVDRDGDGCISLEEFGAISSAFGP--ACDTELRDAFCFFDTDRDGKITA 181

Query: 183 SELHSGFFALGDERCTLDDCRRMIEGVDKTKDGFVCFEDFCRMM 214
            EL+  F A+GD+RCTL+DC+RMI GVDK  DGFVCFEDF RMM
Sbjct: 182 EELNQVFAAIGDDRCTLEDCQRMIAGVDKNGDGFVCFEDFSRMM 218

[Close]

BLAST of Spo04872.1 vs. UniProtKB/TrEMBL
Match: A0A161ZZ87_DAUCA (Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_016967 PE=4 SV=1)

HSP 1 Score: 181.0 bits (458), Expect = 1.500e-42
Identity = 111/210 (52.86%), Postives = 141/210 (67.14%), Query Frame = 1


		  
Query: 5   LKSINPKQIFRLKKPKSSSTITKSEPPSFGSSSYSSTSSSDSPSSATHHHRSGPAPARTQ 64
           L +INPK++F+    K + ++++SE  SFGSS+   TSSSD    +THH +   +   T 
Sbjct: 3   LSNINPKKLFK----KKTRSVSRSEQSSFGSSA---TSSSDD---STHHSKPAGSGTPTS 62

Query: 65  SEDISLRSDAWT-EEAFKMIDLDGDGKITRVELHSLFKRVRVNAPSLSEEEVAAMIKEID 124
                  SD     +AFK+ID DGDGKITR EL SL  RV   +  LSEEE+  M++E+D
Sbjct: 63  VLPDPTSSDFCDLVQAFKIIDADGDGKITRAELGSLLGRV--GSDPLSEEELTMMLRELD 122

Query: 125 IDGDGCISLKELEAVGSALDPTSANEEDLKGAFDFFDTDHDGKISASELHSGFFALGDER 184
            DGDGCISL+E  A+ SA  P S + E L+ AFDFFDTDHDGKISA EL   F ++GD  
Sbjct: 123 SDGDGCISLEEFGAISSAFGPPSGDVE-LREAFDFFDTDHDGKISAEELLCVFTSIGDGV 182

Query: 185 CTLDDCRRMIEGVDKTKDGFVCFEDFCRMM 214
           C+L+DCRRMI+GVDK  DGFVCFEDF +MM
Sbjct: 183 CSLEDCRRMIKGVDKNMDGFVCFEDFSKMM 199

[Close]

BLAST of Spo04872.1 vs. UniProtKB/TrEMBL
Match: B9T832_RICCO (Calcium-binding allergen Ole e, putative OS=Ricinus communis GN=RCOM_0150470 PE=4 SV=1)

HSP 1 Score: 179.9 bits (455), Expect = 3.400e-42
Identity = 117/228 (51.32%), Postives = 152/228 (66.67%), Query Frame = 1


		  
Query: 1   MKQLLKSINPKQIFRLKKPKSSSTITKSEPPSFGSSSYSSTSSSDSPSSATHHHRSG--- 60
           MKQL+K+I+PK++FR KK +S     +S+P      SYSS+SSSDS SS  H  RS    
Sbjct: 1   MKQLIKAISPKRLFRSKKGRS----IRSDP-----LSYSSSSSSDSSSSGFHQKRSADNL 60

Query: 61  ---PAPARTQSEDIS-LRSDAWTE--EAFKMIDLDGDGKITRVELHSLFKRVRVNAPSLS 120
              P+     S D S + SD ++E  +AFK++D D DG ++R +L SL  R+       +
Sbjct: 61  GTPPSVLPDLSGDWSDVSSDFFSELVQAFKLMDNDNDGIVSRTQLESLLIRLG------A 120

Query: 121 EEEVAAMIKEIDIDGDGCISLKELEAV--GSALDPTSANEEDLKGAFDFFDTDHDGKISA 180
           EEEVA M+ E+D DGDGCIS++ L +   G A +P  A +++L+ AF+FFDTDHDGKI+A
Sbjct: 121 EEEVATMLSEVDRDGDGCISVEALMSRIGGPACEP--AGDDELRVAFEFFDTDHDGKITA 180

Query: 181 SELHSGFFALGDERCTLDDCRRMIEGVDKTKDGFVCFEDFCRMMSLPR 218
            EL   + A+GDERCTLDDCRRMI GVDK  DGFVCFEDF RMM L R
Sbjct: 181 EELMGVYKAIGDERCTLDDCRRMIAGVDKNGDGFVCFEDFARMMELQR 211

[Close]

BLAST of Spo04872.1 vs. ExPASy Swiss-Prot
Match: CML36_ARATH (Probable calcium-binding protein CML36 OS=Arabidopsis thaliana GN=CML36 PE=2 SV=1)

HSP 1 Score: 143.7 bits (361), Expect = 2.400e-33
Identity = 96/213 (45.07%), Postives = 123/213 (57.75%), Query Frame = 1


		  
Query: 5   LKSINPKQIFRLKKPKSSSTITKSEPPSFGSSSYSSTSSSDSPSSATHHHRSGPAPARTQ 64
           L  + PK+ F   + K  ST++KS P +F   S SS+S  D  +S          P    
Sbjct: 3   LAKLIPKRFFI--RSKDRSTVSKS-PTAFSFGSASSSSGQDCKNSGGDGGGGSVTPTSIL 62

Query: 65  SEDISLRSDAWTEEAFKMIDLDGDGKITRVELHSLFKRVRVNAPSLSEEEVAAMIKEIDI 124
            E  S  S     +AFK+ID D DG ++R +L SL  R+      L+EEE+  M+KE+D 
Sbjct: 63  PEVPSPYSYVEILQAFKLIDRDNDGAVSRHDLESLLSRL--GPDPLTEEEINVMLKEVDC 122

Query: 125 DGDGCISLKELEAVGSALDPTSANEEDLKGAFDFFDTDHDGKISASELHSGFFALGDERC 184
           DGDG I L+EL +   +LDP   + E LK  F+FFD D DG ISA EL   F  +GDERC
Sbjct: 123 DGDGTIRLEELASRVVSLDPARDSTE-LKETFEFFDADRDGLISADELLRVFSTIGDERC 182

Query: 185 TLDDCRRMIEGVDKTKDGFVCFEDFCRMMSLPR 218
           TLDDC+RMI  VD+  DGFVCF +F RMM L R
Sbjct: 183 TLDDCKRMIADVDEDGDGFVCFTEFSRMMDLQR 209

[Close]

BLAST of Spo04872.1 vs. ExPASy Swiss-Prot
Match: CML35_ARATH (Probable calcium-binding protein CML35 OS=Arabidopsis thaliana GN=CML35 PE=2 SV=2)

HSP 1 Score: 141.7 bits (356), Expect = 9.200e-33
Identity = 92/212 (43.40%), Postives = 130/212 (61.32%), Query Frame = 1


		  
Query: 5   LKSINPKQIFRLKKPKSSSTITKSEPPSFGSSSYSSTSSSDSPSSATHHHRSGPAPARTQ 64
           L  ++PK++FR K   S +++++SEP SF SS+ SS+SS  S  +      + P     Q
Sbjct: 7   LNRLSPKRLFRTK---SKASVSRSEPSSF-SSNASSSSSDGSYGNLKQGPTATPISVLPQ 66

Query: 65  SEDISLRSDAWTE--EAFKMIDLDGDGKITRVELHSLFKRVRVNAPSLSEEEVAAMIKEI 124
           +       D +TE  +AFK+ID D DG ++R +L +L  R+    PS  +EEV+ M++E+
Sbjct: 67  NS-----GDFYTELVQAFKLIDRDDDGVVSRGDLAALISRLSHEPPS--QEEVSLMLREV 126

Query: 125 DIDGDGCISLKELEA-VGSALDPTSANEEDLKGAFDFFDTDHDGKISASELHSGFFALGD 184
           D    GCISL++L + V       S   E+L+  F+ FD D +GKISA ELH  F  +GD
Sbjct: 127 DGGDGGCISLEDLASRVAGTSGEGSVETEELREVFEIFDVDRNGKISAEELHRVFGVIGD 186

Query: 185 ERCTLDDCRRMIEGVDKTKDGFVCFEDFCRMM 214
           ERCTL++C RMI  VD   DGFVCF+DFCRMM
Sbjct: 187 ERCTLEECMRMIATVDGNGDGFVCFDDFCRMM 207

[Close]

BLAST of Spo04872.1 vs. ExPASy Swiss-Prot
Match: CML23_ARATH (Probable calcium-binding protein CML23 OS=Arabidopsis thaliana GN=CML23 PE=2 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 8.600e-23
Identity = 62/141 (43.97%), Postives = 88/141 (62.41%), Query Frame = 1


		  
Query: 77  EEAFKMIDLDGDGKITRVELHSLFKRVRVNAPSLSEEEVAAMIKEIDIDGDGCISLKELE 136
           ++ F+  D + DGKI+  EL  +   +   +P+ S+EE  AM+KE D+DG+G I L E  
Sbjct: 17  KKVFQRFDKNNDGKISIDELKDVIGAL---SPNASQEETKAMMKEFDLDGNGFIDLDEFV 76

Query: 137 AVGSALDPTSANE--EDLKGAFDFFDTDHDGKISASELHSGFFALGDERCTLDDCRRMIE 196
           A+    D +S N    DLK AFD +D D +G+ISA+ELHS    LG E+C++ DC+RMI 
Sbjct: 77  ALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLG-EKCSIQDCQRMIN 136

Query: 197 GVDKTKDGFVCFEDFCRMMSL 216
            VD   DG V FE+F +MM +
Sbjct: 137 KVDSDGDGCVDFEEFKKMMMI 153

[Close]

BLAST of Spo04872.1 vs. ExPASy Swiss-Prot
Match: CML10_ORYSJ (Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica GN=CML10 PE=2 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 7.300e-22
Identity = 70/175 (40.00%), Postives = 99/175 (56.57%), Query Frame = 1


		  
Query: 41  TSSSDSPSSATHHHRSGPAPARTQSEDISLRSDAWTEEAFKMIDLDGDGKITRVELHSLF 100
           + S   P         G +PA T  E++        E  F+  D +GDG+I+R EL +LF
Sbjct: 14  SGSKSPPLPQADPASGGGSPAPTPEEEM--------ERVFRKFDANGDGRISRSELGALF 73

Query: 101 KRVRVNAPSLSEEEVAAMIKEIDIDGDGCISLKELEAVGS-ALDPTSANEEDLKGAFDFF 160
           + +     + +++E+A M+ E D DGDG ISL E  A+ + A    +A EEDL+ AF  F
Sbjct: 74  ESL---GHAATDDELARMMAEADADGDGFISLDEFAALNATASGDAAAVEEDLRHAFRVF 133

Query: 161 DTDHDGKISASELHSGFFALGDERCTLDDCRRMIEGVDKTKDGFVCFEDFCRMMS 215
           D D +G ISA+EL      LG E+ T+  CRRMIEGVD+  DG + FE+F  MM+
Sbjct: 134 DADGNGTISAAELARVLHGLG-EKATVQQCRRMIEGVDQNGDGLISFEEFKVMMA 176

[Close]

BLAST of Spo04872.1 vs. ExPASy Swiss-Prot
Match: CML15_ORYSJ (Probable calcium-binding protein CML15 OS=Oryza sativa subsp. japonica GN=CML15 PE=2 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 3.100e-20
Identity = 71/183 (38.80%), Postives = 105/183 (57.38%), Query Frame = 1


		  
Query: 34  GSSSYSSTSSSDSPSSATHHHRSGPAPARTQSEDISLRSDAWTEEAFKMIDLDGDGKITR 93
           G+SS  S S  ++  +     R     A   ++     ++  TE  F+  D +GDG+I+R
Sbjct: 14  GNSSGRSRSMREAAMNVDWSPRPSDLAAAAAAKPRPPAAEDETERVFRKFDANGDGRISR 73

Query: 94  VELHSLFKRVRVNAPSLSEEEVAAMIKEIDIDGDGCISLKELEAVGSALDP--TSANEED 153
            EL +LF+ V     +++++EVA M++E D DGDG ISL E  A+ SA  P   +A EED
Sbjct: 74  AELAALFRSV---GHAVTDDEVARMMQEADSDGDGYISLGEFAAI-SAPPPGDAAAAEED 133

Query: 154 LKGAFDFFDTDHDGKISASELHSGFFALGDERCTLDDCRRMIEGVDKTKDGFVCFEDFCR 213
           L+ AF  FD D +G I+ +EL      +G E  T+  CRRMI+GVD+  DG + FE+F  
Sbjct: 134 LRHAFGVFDADGNGVITPAELARVLRGIG-EAATVAQCRRMIDGVDRNGDGLINFEEFKL 191

Query: 214 MMS 215
           MM+
Sbjct: 194 MMA 191

[Close]

BLAST of Spo04872.1 vs. TAIR (Arabidopsis)
Match: AT3G10190.1 (Calcium-binding EF-hand family protein)

HSP 1 Score: 143.7 bits (361), Expect = 1.400e-34
Identity = 96/213 (45.07%), Postives = 123/213 (57.75%), Query Frame = 1


		  
Query: 5   LKSINPKQIFRLKKPKSSSTITKSEPPSFGSSSYSSTSSSDSPSSATHHHRSGPAPARTQ 64
           L  + PK+ F   + K  ST++KS P +F   S SS+S  D  +S          P    
Sbjct: 3   LAKLIPKRFFI--RSKDRSTVSKS-PTAFSFGSASSSSGQDCKNSGGDGGGGSVTPTSIL 62

Query: 65  SEDISLRSDAWTEEAFKMIDLDGDGKITRVELHSLFKRVRVNAPSLSEEEVAAMIKEIDI 124
            E  S  S     +AFK+ID D DG ++R +L SL  R+      L+EEE+  M+KE+D 
Sbjct: 63  PEVPSPYSYVEILQAFKLIDRDNDGAVSRHDLESLLSRL--GPDPLTEEEINVMLKEVDC 122

Query: 125 DGDGCISLKELEAVGSALDPTSANEEDLKGAFDFFDTDHDGKISASELHSGFFALGDERC 184
           DGDG I L+EL +   +LDP   + E LK  F+FFD D DG ISA EL   F  +GDERC
Sbjct: 123 DGDGTIRLEELASRVVSLDPARDSTE-LKETFEFFDADRDGLISADELLRVFSTIGDERC 182

Query: 185 TLDDCRRMIEGVDKTKDGFVCFEDFCRMMSLPR 218
           TLDDC+RMI  VD+  DGFVCF +F RMM L R
Sbjct: 183 TLDDCKRMIADVDEDGDGFVCFTEFSRMMDLQR 209

[Close]

BLAST of Spo04872.1 vs. TAIR (Arabidopsis)
Match: AT2G41410.1 (Calcium-binding EF-hand family protein)

HSP 1 Score: 141.7 bits (356), Expect = 5.200e-34
Identity = 92/212 (43.40%), Postives = 130/212 (61.32%), Query Frame = 1


		  
Query: 5   LKSINPKQIFRLKKPKSSSTITKSEPPSFGSSSYSSTSSSDSPSSATHHHRSGPAPARTQ 64
           L  ++PK++FR K   S +++++SEP SF SS+ SS+SS  S  +      + P     Q
Sbjct: 7   LNRLSPKRLFRTK---SKASVSRSEPSSF-SSNASSSSSDGSYGNLKQGPTATPISVLPQ 66

Query: 65  SEDISLRSDAWTE--EAFKMIDLDGDGKITRVELHSLFKRVRVNAPSLSEEEVAAMIKEI 124
           +       D +TE  +AFK+ID D DG ++R +L +L  R+    PS  +EEV+ M++E+
Sbjct: 67  NS-----GDFYTELVQAFKLIDRDDDGVVSRGDLAALISRLSHEPPS--QEEVSLMLREV 126

Query: 125 DIDGDGCISLKELEA-VGSALDPTSANEEDLKGAFDFFDTDHDGKISASELHSGFFALGD 184
           D    GCISL++L + V       S   E+L+  F+ FD D +GKISA ELH  F  +GD
Sbjct: 127 DGGDGGCISLEDLASRVAGTSGEGSVETEELREVFEIFDVDRNGKISAEELHRVFGVIGD 186

Query: 185 ERCTLDDCRRMIEGVDKTKDGFVCFEDFCRMM 214
           ERCTL++C RMI  VD   DGFVCF+DFCRMM
Sbjct: 187 ERCTLEECMRMIATVDGNGDGFVCFDDFCRMM 207

[Close]

BLAST of Spo04872.1 vs. TAIR (Arabidopsis)
Match: AT1G66400.1 (calmodulin like 23)

HSP 1 Score: 108.6 bits (270), Expect = 4.900e-24
Identity = 62/141 (43.97%), Postives = 88/141 (62.41%), Query Frame = 1


		  
Query: 77  EEAFKMIDLDGDGKITRVELHSLFKRVRVNAPSLSEEEVAAMIKEIDIDGDGCISLKELE 136
           ++ F+  D + DGKI+  EL  +   +   +P+ S+EE  AM+KE D+DG+G I L E  
Sbjct: 17  KKVFQRFDKNNDGKISIDELKDVIGAL---SPNASQEETKAMMKEFDLDGNGFIDLDEFV 76

Query: 137 AVGSALDPTSANE--EDLKGAFDFFDTDHDGKISASELHSGFFALGDERCTLDDCRRMIE 196
           A+    D +S N    DLK AFD +D D +G+ISA+ELHS    LG E+C++ DC+RMI 
Sbjct: 77  ALFQISDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLG-EKCSIQDCQRMIN 136

Query: 197 GVDKTKDGFVCFEDFCRMMSL 216
            VD   DG V FE+F +MM +
Sbjct: 137 KVDSDGDGCVDFEEFKKMMMI 153

[Close]

BLAST of Spo04872.1 vs. TAIR (Arabidopsis)
Match: AT5G37770.1 (EF hand calcium-binding protein family)

HSP 1 Score: 99.8 bits (247), Expect = 2.300e-21
Identity = 59/142 (41.55%), Postives = 87/142 (61.27%), Query Frame = 1


		  
Query: 77  EEAFKMIDLDGDGKITRVELHSLFKRVRVNAPSLSEEEVAAMIKEIDIDGDGCISLKELE 136
           ++ F+  D +GDGKI+  EL  +   +R  +P+ S EE   M+K+ D+DG+G I L E  
Sbjct: 19  KKVFQRFDKNGDGKISVDELKEV---IRALSPTASPEETVTMMKQFDLDGNGFIDLDEFV 78

Query: 137 A---VGSALDPTSANE-EDLKGAFDFFDTDHDGKISASELHSGFFALGDERCTLDDCRRM 196
           A   +G      + N+  DLK AF+ +D D +G+ISA ELHS    LG E+C++ DC++M
Sbjct: 79  ALFQIGIGGGGNNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLG-EKCSVQDCKKM 138

Query: 197 IEGVDKTKDGFVCFEDFCRMMS 215
           I  VD   DG V F++F +MMS
Sbjct: 139 ISKVDIDGDGCVNFDEFKKMMS 156

[Close]

BLAST of Spo04872.1 vs. TAIR (Arabidopsis)
Match: AT1G24620.1 (EF hand calcium-binding protein family)

HSP 1 Score: 98.2 bits (243), Expect = 6.600e-21
Identity = 58/139 (41.73%), Postives = 82/139 (58.99%), Query Frame = 1


		  
Query: 77  EEAFKMIDLDGDGKITRVELHSLFKRVRVNAPSLSEEEVAAMIKEIDIDGDGCISLKELE 136
           E  FK  D++GDGKI+  EL ++   +    P   EEE+   I EID  GDG I+ +E  
Sbjct: 39  EAVFKKFDVNGDGKISSKELGAIMTSLGHEVP---EEELEKAITEIDRKGDGYINFEEFV 98

Query: 137 AVGSALDPTSANEEDLKGAFDFFDTDHDGKISASELHSGFFALGDERCTLDDCRRMIEGV 196
            + +     +   E+LK AF  +D D +G ISA ELH    +LGDE C++ +CR+MI GV
Sbjct: 99  ELNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDE-CSIAECRKMIGGV 158

Query: 197 DKTKDGFVCFEDFCRMMSL 216
           DK  DG + FE+F  MM++
Sbjct: 159 DKDGDGTIDFEEFKIMMTM 173

The following BLAST results are available for this feature:

BLAST of Spo04872.1 vs. NCBI nr
BLAST of Spo04872.1 vs. UniProtKB/TrEMBL
BLAST of Spo04872.1 vs. ExPASy Swiss-Prot
BLAST of Spo04872.1 vs. TAIR (Arabidopsis)

BLAST of Spo04872.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902226899\|gb\|KNA20519.1\|	1.3e-114	100.	hypothetical protein SOVF_0515...	[more]
gi\|731324427\|ref\|XP_010672963.1\|	4.5e-80	74.0	PREDICTED: probable calcium-bi...	[more]
gi\|225441325\|ref\|XP_002276334.1\|	5.7e-43	52.2	PREDICTED: probable calcium-bi...	[more]
gi\|697172930\|ref\|XP_009595400.1\|	1.7e-42	50.2	PREDICTED: probable calcium-bi...	[more]
gi\|1021035939\|gb\|KZM93722.1\|	2.2e-42	52.8	hypothetical protein DCAR_0169...	[more]

BLAST of Spo04872.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9RNM0_SPIOL	8.7e-115	100.	Uncharacterized protein OS=Spi...	[more]
A0A0J8FGX1_BETVU	3.1e-80	74.0	Uncharacterized protein OS=Bet...	[more]
F6HLP0_VITVI	4.0e-43	52.2	Putative uncharacterized prote...	[more]
A0A161ZZ87_DAUCA	1.5e-42	52.8	Uncharacterized protein OS=Dau...	[more]
B9T832_RICCO	3.4e-42	51.3	Calcium-binding allergen Ole e...	[more]

BLAST of Spo04872.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
CML36_ARATH	2.4e-33	45.0	Probable calcium-binding prote...	[more]
CML35_ARATH	9.2e-33	43.4	Probable calcium-binding prote...	[more]
CML23_ARATH	8.6e-23	43.9	Probable calcium-binding prote...	[more]
CML10_ORYSJ	7.3e-22	40.0	Probable calcium-binding prote...	[more]
CML15_ORYSJ	3.1e-20	38.8	Probable calcium-binding prote...	[more]

BLAST of Spo04872.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 5


Match Name	E-value	Identity	Description
AT3G10190.1	1.4e-34	45.0	Calcium-binding EF-hand family...	[more]
AT2G41410.1	5.2e-34	43.4	Calcium-binding EF-hand family...	[more]
AT1G66400.1	4.9e-24	43.9	calmodulin like 23	[more]
AT5G37770.1	2.3e-21	41.5	EF hand calcium-binding protei...	[more]
AT1G24620.1	6.6e-21	41.7	EF hand calcium-binding protei...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 149..213 score: 1.1E-8coord: 76..136 score: 5.0
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 188..216 score: 0.05coord: 151..179 score: 7.0E-4coord: 75..103 score: 0.0013coord: 114..142 score: 3.
IPR002048	EF-hand domain	PROFILE	PS50222	EF_HAND_2	coord: 71..106 score: 12.951coord: 184..217 score: 9.827coord: 147..182 score: 14.625coord: 110..145 score: 12
IPR011992	EF-hand domain pair	GENE3D	1.10.238.10		coord: 74..159 score: 6.3E-25coord: 160..214 score: 2.9
IPR011992	EF-hand domain pair	unknown	SSF47473	EF-hand	coord: 63..214 score: 5.08
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 123..135 score: -coord: 84..96 score: -coord: 160..172 scor
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 77..213 score: 9.6

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding