Spo05258.1 (mRNA) | SpinachBase

Overview


Name	Spo05258.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	Cytochrome P450
Location	chr5 : 12435787 .. 12439486 (+)
Sequence length	2327

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTTCTATCCATAAAATATCTTTCTAGTAACTCCCTTCTCTATATGGCTATATATGTAACAACCTCCCAAGACTCACATGCACACCTAACACTTAACAAAAAATCCTACCTTTCATTTCATCTTCATTTTAGCATTGCTTACTATTTTTGTACATCTTTCTGCCCAAAACATGTCACCCTTAGAAATAATCTCTTACACTATTGCCATTGCCATTGCCATTGCCATCATCACCTACTACCTCATTACCACCATCATCTTTCGACACCCCCGGCCGCTTCCGCCGGGGCCAAAGCCATGGCCAATAGTCGGAAACCTCCCACACATGGGCCCAGTCCCACACCAGGGACTGGCAGCCCTGGCTCAAAAGTATGGGCCACTCCTCTACATGAGACTAGGCTACGTCCATGTAGTGGTGGCGGCGTCGGCTTCGGTGGCGGCGCAGTTTTTAAAAACTCATGATGCAAATTTCTCAAGTAGGCCTCCAAATTCAGGGGCAAAGCATATAGCATATAACTACCAAGACATGGTGTTTGCACCTTATGGTCAAAGATGGAGGCTTCTTAGGAAGATTTCTTCACTTCATTTGTTCTCTACTAAGGCCATGGAAGACTACAAACCAATTCGACAGGTTTTTATTTTCTAACTTCTATTTTTTTTAATGTTGCTTACAATTTTTATTTATCTTTATGAGAAAAATCCATGTTGCATCATTTTGTAGATCTAAGATAAATTGAAATTAATGTGTGAATTTTTTGTGAGATTGTTCTACATTGAAGAAAGATAAGAAGGATGACCGACATAAAAACCGGTTTAATATTTTAATTGGACCAATAAAATGGAGTTAGACAGAGATTGAGACAAAATTGTGAGATAGATGAGCTACATATATAGATTTAGGAAGGTACCATGGAAGGCTTGAACATGCCAATGATGATTACAGTCAGTTTTTAGTTTTGGTTTTATGAGTTAGAAATTAATATGATAGAGGTAGACTTATAATCTTAAAGTTAGTTATATTCATAATACTCGTGGCTGATTTGAAAACTGACAACAAAAACTAAAATTACTTTACTCCGTATGTTGTTTCCGTATTTTTGGTTTTTAGTGAATCGGGTTCTTAACTAGCTGTTTACTAGTTAATTATGCATTCTTAGCCGGAATTGACCTTACATGTTAATTGTCATGTGATAGAGCTGATAACGTGACAACTAAATTTGAATGGGAAGCATATATGTATTTGGAATTCATGTAAATTGACATAAATTTTTATCTTTGATTTGTCCAAATTTGTGAAACATATAGGAGGAAGTGGGCATACTAACAAAGGCACTAGTAAAGGCAGAATCAAAAGAAGCAGTTCAACTAGGACAACTTTTGAATGTGTGCACCACCAATGCTCTCTCAAGGGTGATGCTAGGAAAGAGAGTGTTCGGCGACGGCACCGGCGAAAGTGATAAAAAATCAGAGGAATTCAAGGATATGGTAATGGAACTCATGGTTCTCGCCGGAGAATTTAACATCGGCGACTTTGTTCCGGCGTTGGAATGGTTAGACTTACAAGGGATTGCTACTAAGATGAAAAAACTTCATAAGAGGTTTGACAATTTTCTTGGTGCCATTTTGGAAGAACACAAGGCTAAATTTAAGGGTGGTAATTTTGGTGGGGATTTCCTTAGCACTTTGATCTCTTTAAAAGATAATTGTGATGGTGAGGGTGGCAAGTTGACTGACATTGAAATCAAGGCCTTACTCTTGGTAATTATCTCTACATTTTTCTTTGTATTTCACTATAAATGTTTATTTTTGTTAATGGAAATGGTCAAAAGTTCAAACAAAAATGGTATAACCGGTAGTATAATAAAAATTATCGGGAGGACGGTTAGAGATAATAGTTAGAGACTAGTGAGGATAAAATGATAAATAAAACTTATAGAGTATTAGCTACGGTATTTTGAAATAAAACAGGGAAAGATAGACAATGCTAAAAGAAAATTTTAAATGGATTAACATATTCAATTTGTGAAACATATAAGAAAAAAAATCATGTAATTATAATCGCCTCAACTCTCAATATAATGTTAAAAATTTCATTTTTTTTTGTAATTTTATACTTCCTCCGTCTTTTAATACTCGCAACGTTTGGACTTTTGCCACTATTCATATAATCTACTTTGACTATTCTTAGTATTTTTTATATAAGATAAAACATAGTCACGTGGGATCTTGTTAGATTCGTCTCAATGTGTATTTTCAAAATATCAACTTTTTATAATTTTTGCATAAAGAGAATTTAAGATATAAATGATCAAAGTTGTGCATTGGCATGCGTGAAACTAACAAACGTTGCGAGTATTAAAAGACGGAGGAAGTACGAAGTATAGATTAATTTCTCTTGTTCTTAACCTTGGCTTTTGTGAATTTATTGGTTTTGGTTGAACTTTTATGTGGTGGCAGAATATGTTCACAGCAGGGACTGACACGTCATCAAGTACAACAGAATGGGCCATAGCAGAACTAATCCGCCATCCCAAAATCTTGGCCCATGTTCAACAAGAGCTAGACTCAATCGTGGGTCAAGACCGACTTGTTAACGAATCCGACCTACCGAACTTACCTTACTTCCAAGCCGTGATGAAAGAGGTATTCCGGCTCCACCCATCGACACCACTCTCCCTCCCTCGAGTCTCGGCCGAGCCATGTGAAATCAATGGTTACTACATTCCTAAAAACACTACTCTCTTGGTCAATGTATGGGCCATAGCGCGTGATCCAAATGTTTGGGCCGACCCACTAGAGTTCAGGCCTGAAAGGTTCTTAAAAGGCGGGGAGAGGCCCAACGCAGATGTTAAGGGTAATGATTTTGAGGTGATCCCGTTTGGGGCCGGGCGGAGGATATGCGCCGGGATGAGCTTAGGGCTTCGTATGGTCCAATTATTGACAGCCGCTTTGGTCCATGGTTTTGACTGGAAGTTAGCCCATGGGCTTGCACAAGAAAAGCTGAACATGGAAGAGGCTTATGGGCTGACGTTGCAACGTAAGGTTCCCTTGATGGTCTACCCGCTACCTCGGCTGCCAGCCAAAGCCTATACCACAACTTACTAAAGTGGTGCGTCCGTTATTAAAATTTGTTGCTTACACGAGACAAGGGACGAAATGACGCCTACCAATAGTACCAGTCCATGCTGAGCTTGGTTGTAACACGACTTATACGAGTATTAGATTTATGCATGTGTGTGCTCCTTGTAATTTCTGTAAAATACCAAGGTGAATGAGGTAAAATGCTTTGCTTACTTGATAATCAACTCAACTTCTATTGGTTAAAAATAATGAGCCACCAAGGTTACAATGTTTGAGCCTCACTGCGGCAGAAGCTGCAAACATGCATCTAGTAGTCCCTTAATCATCCTGCAGTTTACTCGGCAGCGCCACTGTTGAGACACTTTTCCAGCCTGTTAAGACTATACAAGGACTGTATGTATGGGTGCGAAAGTGCTTATTTTAAAACAACGAGAAATAAGGCCCTTTTATAGTTTTATGTAAATGTAGACATCCTTGTACTCTACTATTTTACAAGTGAAAATCTTCACAATATTTTCTTCTTTTTTCTTTAGAGAAGAAAAACTACATTACAACCATTTGTCCATGACAAATTATTACATGAAAATAACCTAGTGGAGCACATATCAAAACTATTACCAG

mRNA sequence

TTTTCTATCCATAAAATATCTTTCTAGTAACTCCCTTCTCTATATGGCTATATATGTAACAACCTCCCAAGACTCACATGCACACCTAACACTTAACAAAAAATCCTACCTTTCATTTCATCTTCATTTTAGCATTGCTTACTATTTTTGTACATCTTTCTGCCCAAAACATGTCACCCTTAGAAATAATCTCTTACACTATTGCCATTGCCATTGCCATTGCCATCATCACCTACTACCTCATTACCACCATCATCTTTCGACACCCCCGGCCGCTTCCGCCGGGGCCAAAGCCATGGCCAATAGTCGGAAACCTCCCACACATGGGCCCAGTCCCACACCAGGGACTGGCAGCCCTGGCTCAAAAGTATGGGCCACTCCTCTACATGAGACTAGGCTACGTCCATGTAGTGGTGGCGGCGTCGGCTTCGGTGGCGGCGCAGTTTTTAAAAACTCATGATGCAAATTTCTCAAGTAGGCCTCCAAATTCAGGGGCAAAGCATATAGCATATAACTACCAAGACATGGTGTTTGCACCTTATGGTCAAAGATGGAGGCTTCTTAGGAAGATTTCTTCACTTCATTTGTTCTCTACTAAGGCCATGGAAGACTACAAACCAATTCGACAGGAGGAAGTGGGCATACTAACAAAGGCACTAGTAAAGGCAGAATCAAAAGAAGCAGTTCAACTAGGACAACTTTTGAATGTGTGCACCACCAATGCTCTCTCAAGGGTGATGCTAGGAAAGAGAGTGTTCGGCGACGGCACCGGCGAAAGTGATAAAAAATCAGAGGAATTCAAGGATATGGTAATGGAACTCATGGTTCTCGCCGGAGAATTTAACATCGGCGACTTTGTTCCGGCGTTGGAATGGTTAGACTTACAAGGGATTGCTACTAAGATGAAAAAACTTCATAAGAGGTTTGACAATTTTCTTGGTGCCATTTTGGAAGAACACAAGGCTAAATTTAAGGGTGGTAATTTTGGTGGGGATTTCCTTAGCACTTTGATCTCTTTAAAAGATAATTGTGATGGTGAGGGTGGCAAGTTGACTGACATTGAAATCAAGGCCTTACTCTTGAATATGTTCACAGCAGGGACTGACACGTCATCAAGTACAACAGAATGGGCCATAGCAGAACTAATCCGCCATCCCAAAATCTTGGCCCATGTTCAACAAGAGCTAGACTCAATCGTGGGTCAAGACCGACTTGTTAACGAATCCGACCTACCGAACTTACCTTACTTCCAAGCCGTGATGAAAGAGGTATTCCGGCTCCACCCATCGACACCACTCTCCCTCCCTCGAGTCTCGGCCGAGCCATGTGAAATCAATGGTTACTACATTCCTAAAAACACTACTCTCTTGGTCAATGTATGGGCCATAGCGCGTGATCCAAATGTTTGGGCCGACCCACTAGAGTTCAGGCCTGAAAGGTTCTTAAAAGGCGGGGAGAGGCCCAACGCAGATGTTAAGGGTAATGATTTTGAGGTGATCCCGTTTGGGGCCGGGCGGAGGATATGCGCCGGGATGAGCTTAGGGCTTCGTATGGTCCAATTATTGACAGCCGCTTTGGTCCATGGTTTTGACTGGAAGTTAGCCCATGGGCTTGCACAAGAAAAGCTGAACATGGAAGAGGCTTATGGGCTGACGTTGCAACGTAAGGTTCCCTTGATGGTCTACCCGCTACCTCGGCTGCCAGCCAAAGCCTATACCACAACTTACTAAAGTGGTGCGTCCGTTATTAAAATTTGTTGCTTACACGAGACAAGGGACGAAATGACGCCTACCAATAGTACCAGTCCATGCTGAGCTTGGTTGTAACACGACTTATACGAGTATTAGATTTATGCATGTGTGTGCTCCTTGTAATTTCTGTAAAATACCAAGGTGAATGAGGTAAAATGCTTTGCTTACTTGATAATCAACTCAACTTCTATTGGTTAAAAATAATGAGCCACCAAGGTTACAATGTTTGAGCCTCACTGCGGCAGAAGCTGCAAACATGCATCTAGTAGTCCCTTAATCATCCTGCAGTTTACTCGGCAGCGCCACTGTTGAGACACTTTTCCAGCCTGTTAAGACTATACAAGGACTGTATGTATGGGTGCGAAAGTGCTTATTTTAAAACAACGAGAAATAAGGCCCTTTTATAGTTTTATGTAAATGTAGACATCCTTGTACTCTACTATTTTACAAGTGAAAATCTTCACAATATTTTCTTCTTTTTTCTTTAGAGAAGAAAAACTACATTACAACCATTTGTCCATGACAAATTATTACATGAAAATAACCTAGTGGAGCACATATCAAAACTATTACCAG

Coding sequence (CDS)

ATGTCACCCTTAGAAATAATCTCTTACACTATTGCCATTGCCATTGCCATTGCCATCATCACCTACTACCTCATTACCACCATCATCTTTCGACACCCCCGGCCGCTTCCGCCGGGGCCAAAGCCATGGCCAATAGTCGGAAACCTCCCACACATGGGCCCAGTCCCACACCAGGGACTGGCAGCCCTGGCTCAAAAGTATGGGCCACTCCTCTACATGAGACTAGGCTACGTCCATGTAGTGGTGGCGGCGTCGGCTTCGGTGGCGGCGCAGTTTTTAAAAACTCATGATGCAAATTTCTCAAGTAGGCCTCCAAATTCAGGGGCAAAGCATATAGCATATAACTACCAAGACATGGTGTTTGCACCTTATGGTCAAAGATGGAGGCTTCTTAGGAAGATTTCTTCACTTCATTTGTTCTCTACTAAGGCCATGGAAGACTACAAACCAATTCGACAGGAGGAAGTGGGCATACTAACAAAGGCACTAGTAAAGGCAGAATCAAAAGAAGCAGTTCAACTAGGACAACTTTTGAATGTGTGCACCACCAATGCTCTCTCAAGGGTGATGCTAGGAAAGAGAGTGTTCGGCGACGGCACCGGCGAAAGTGATAAAAAATCAGAGGAATTCAAGGATATGGTAATGGAACTCATGGTTCTCGCCGGAGAATTTAACATCGGCGACTTTGTTCCGGCGTTGGAATGGTTAGACTTACAAGGGATTGCTACTAAGATGAAAAAACTTCATAAGAGGTTTGACAATTTTCTTGGTGCCATTTTGGAAGAACACAAGGCTAAATTTAAGGGTGGTAATTTTGGTGGGGATTTCCTTAGCACTTTGATCTCTTTAAAAGATAATTGTGATGGTGAGGGTGGCAAGTTGACTGACATTGAAATCAAGGCCTTACTCTTGAATATGTTCACAGCAGGGACTGACACGTCATCAAGTACAACAGAATGGGCCATAGCAGAACTAATCCGCCATCCCAAAATCTTGGCCCATGTTCAACAAGAGCTAGACTCAATCGTGGGTCAAGACCGACTTGTTAACGAATCCGACCTACCGAACTTACCTTACTTCCAAGCCGTGATGAAAGAGGTATTCCGGCTCCACCCATCGACACCACTCTCCCTCCCTCGAGTCTCGGCCGAGCCATGTGAAATCAATGGTTACTACATTCCTAAAAACACTACTCTCTTGGTCAATGTATGGGCCATAGCGCGTGATCCAAATGTTTGGGCCGACCCACTAGAGTTCAGGCCTGAAAGGTTCTTAAAAGGCGGGGAGAGGCCCAACGCAGATGTTAAGGGTAATGATTTTGAGGTGATCCCGTTTGGGGCCGGGCGGAGGATATGCGCCGGGATGAGCTTAGGGCTTCGTATGGTCCAATTATTGACAGCCGCTTTGGTCCATGGTTTTGACTGGAAGTTAGCCCATGGGCTTGCACAAGAAAAGCTGAACATGGAAGAGGCTTATGGGCTGACGTTGCAACGTAAGGTTCCCTTGATGGTCTACCCGCTACCTCGGCTGCCAGCCAAAGCCTATACCACAACTTACTAA

Protein sequence

MSPLEIISYTIAIAIAIAIITYYLITTIIFRHPRPLPPGPKPWPIVGNLPHMGPVPHQGLAALAQKYGPLLYMRLGYVHVVVAASASVAAQFLKTHDANFSSRPPNSGAKHIAYNYQDMVFAPYGQRWRLLRKISSLHLFSTKAMEDYKPIRQEEVGILTKALVKAESKEAVQLGQLLNVCTTNALSRVMLGKRVFGDGTGESDKKSEEFKDMVMELMVLAGEFNIGDFVPALEWLDLQGIATKMKKLHKRFDNFLGAILEEHKAKFKGGNFGGDFLSTLISLKDNCDGEGGKLTDIEIKALLLNMFTAGTDTSSSTTEWAIAELIRHPKILAHVQQELDSIVGQDRLVNESDLPNLPYFQAVMKEVFRLHPSTPLSLPRVSAEPCEINGYYIPKNTTLLVNVWAIARDPNVWADPLEFRPERFLKGGERPNADVKGNDFEVIPFGAGRRICAGMSLGLRMVQLLTAALVHGFDWKLAHGLAQEKLNMEEAYGLTLQRKVPLMVYPLPRLPAKAYTTTY

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo05258	Spo05258	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo05258.1	Spo05258.1-protein	polypeptide

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo05258.1.exon.1	Spo05258.1.exon.1	exon
Spo05258.1.exon.2	Spo05258.1.exon.2	exon
Spo05258.1.exon.3	Spo05258.1.exon.3	exon

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo05258.1.utr5p.1	Spo05258.1.utr5p.1	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo05258.1.CDS.1	Spo05258.1.CDS.1	CDS
Spo05258.1.CDS.2	Spo05258.1.CDS.2	CDS
Spo05258.1.CDS.3	Spo05258.1.CDS.3	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo05258.1.utr3p.1	Spo05258.1.utr3p.1	three_prime_UTR

Homology

[Close]

BLAST of Spo05258.1 vs. NCBI nr
Match: gi|731366673|ref|XP_010695197.1| (PREDICTED: flavonoid 3'-monooxygenase [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 892.5 bits (2305), Expect = 3.500e-256
Identity = 446/515 (86.60%), Postives = 471/515 (91.46%), Query Frame = 1

		  
Query: 1   MSPLEIISYTIAIAIAIAIITYYLITTIIFRHPRPLPPGPKPWPIVGNLPHMGPVPHQGL 60
           MSPLE+  YTIA+AI I    YYLITTI  R+P PLPPGPKPWPIVGNLPHMGPVPHQGL
Sbjct: 1   MSPLELTFYTIALAIFI----YYLITTIFSRYPGPLPPGPKPWPIVGNLPHMGPVPHQGL 60

Query: 61  AALAQKYGPLLYMRLGYVHVVVAASASVAAQFLKTHDANFSSRPPNSGAKHIAYNYQDMV 120
           A LAQKYGPLL++RLGYVHVVVAASASVAA+FLK HDANFSSRPPNSGAK+IAYNYQD+V
Sbjct: 61  AKLAQKYGPLLHLRLGYVHVVVAASASVAAEFLKAHDANFSSRPPNSGAKYIAYNYQDLV 120

Query: 121 FAPYGQRWRLLRKISSLHLFSTKAMEDYKPIRQEEVGILTKALVKAESKEAVQLGQLLNV 180
           FAPYG RWR+LRKISSLHLFS KA+EDYKPIRQEEV ILTKAL K+ S   +QLGQLLNV
Sbjct: 121 FAPYGPRWRMLRKISSLHLFSAKALEDYKPIRQEEVAILTKALAKSGSNNPIQLGQLLNV 180

Query: 181 CTTNALSRVMLGKRVFGDGTGESDKKSEEFKDMVMELMVLAGEFNIGDFVPALEWLDLQG 240
           CTTNALSR MLG+RVFGDGTG+SD KSEEFKDMVME+MVLAGEFNIGDFVPALEWLDLQG
Sbjct: 181 CTTNALSRAMLGRRVFGDGTGKSDTKSEEFKDMVMEVMVLAGEFNIGDFVPALEWLDLQG 240

Query: 241 IATKMKKLHKRFDNFLGAILEEHKAKFKGGNFGGDFLSTLISLKDNCDGEGGKLTDIEIK 300
           IA+KMKKLHKRFD FLGAILEEHK   K G+ G DFLSTLISLKDN D E GKLTD EIK
Sbjct: 241 IASKMKKLHKRFDTFLGAILEEHKTNPK-GDHGRDFLSTLISLKDNYDDEDGKLTDTEIK 300

Query: 301 ALLLNMFTAGTDTSSSTTEWAIAELIRHPKILAHVQQELDSIVGQDRLVNESDLPNLPYF 360
           ALLLNMFTAGTDTSSSTTEWAIAELIRHPKIL  VQQELDSIVGQDRLVNESDLPNLPYF
Sbjct: 301 ALLLNMFTAGTDTSSSTTEWAIAELIRHPKILDKVQQELDSIVGQDRLVNESDLPNLPYF 360

Query: 361 QAVMKEVFRLHPSTPLSLPRVSAEPCEINGYYIPKNTTLLVNVWAIARDPNVWADPLEFR 420
           QA++KEVFRLHPSTPLSLPR+SA+PCEINGYYIPKNTTLLVNVWAIARDPNVW+ PLEF+
Sbjct: 361 QAMIKEVFRLHPSTPLSLPRISADPCEINGYYIPKNTTLLVNVWAIARDPNVWSSPLEFQ 420

Query: 421 PERFLKGGERPNADVKGNDFEVIPFGAGRRICAGMSLGLRMVQLLTAALVHGFDWKLAHG 480
           PERFL GGERPNADVKGNDFEVIPFGAGRRICAGMSLGLRMVQLLTA LVHGFDWKLA G
Sbjct: 421 PERFLPGGERPNADVKGNDFEVIPFGAGRRICAGMSLGLRMVQLLTATLVHGFDWKLADG 480

Query: 481 LAQEKLNMEEAYGLTLQRKVPLMVYPLPRLPAKAY 516
           L  EKLNMEEAYGLTLQRK+PL V+PLPRL A AY
Sbjct: 481 LMSEKLNMEEAYGLTLQRKIPLAVHPLPRLSAHAY 510

[Close]

BLAST of Spo05258.1 vs. NCBI nr
Match: gi|1005743021|gb|AMQ23620.1| (flavonoid 3'hydroxylase [Silene littorea])

HSP 1 Score: 824.7 bits (2129), Expect = 9.000e-236
Identity = 410/518 (79.15%), Postives = 455/518 (87.84%), Query Frame = 1

		  
Query: 1   MSPLEIISYTIAIAIAIAIITYYLITTIIFRHPRPLPPGPKPWPIVGNLPHMGPVPHQGL 60
           MS LE+  YTIAIA  +    YYL++T   R+P+PLPPGPKPWPIVGNLPHMGP PHQGL
Sbjct: 1   MSTLELTFYTIAIATLL----YYLLSTTFHRYPKPLPPGPKPWPIVGNLPHMGPAPHQGL 60

Query: 61  AALAQKYGPLLYMRLGYVHVVVAASASVAAQFLKTHDANFSSRPPNSGAKHIAYNYQDMV 120
           AALAQKYGPLLYMRLGYVHVVVAASASVA+QFLK HD NFSSRPPNSGAKHIAYNYQD+V
Sbjct: 61  AALAQKYGPLLYMRLGYVHVVVAASASVASQFLKVHDVNFSSRPPNSGAKHIAYNYQDLV 120

Query: 121 FAPYGQRWRLLRKISSLHLFSTKAMEDYKPIRQEEVGILTKALVKAESKEAVQLGQLLNV 180
           FAPYG +WR+LRKI S+H+FS KA++D   +RQEE  IL  AL  A++K  VQLGQLLNV
Sbjct: 121 FAPYGPKWRMLRKICSVHMFSNKALDDVTSVRQEEASILVNALANADAKP-VQLGQLLNV 180

Query: 181 CTTNALSRVMLGKRVFGDGTGESDKKSEEFKDMVMELMVLAGEFNIGDFVPALEWLDLQG 240
           CTTNALSRVMLG+RV GDGTG+SD K+EEFK+MVMELMVL G FNIGDFVP LE LDLQG
Sbjct: 181 CTTNALSRVMLGRRVLGDGTGKSDPKAEEFKEMVMELMVLTGVFNIGDFVPVLECLDLQG 240

Query: 241 IATKMKKLHKRFDNFLGAILEEHKAKFKGGNFGGDFLSTLISLKDNCDGEGGKLTDIEIK 300
           IA+KMK LHKR DNF+GAILEEHKA  K G  GGD LSTLISLKDN DGEGG+ TD EIK
Sbjct: 241 IASKMKNLHKRLDNFMGAILEEHKANGKHG--GGDLLSTLISLKDNYDGEGGQFTDTEIK 300

Query: 301 ALLLNMFTAGTDTSSSTTEWAIAELIRHPKILAHVQQELDSIVGQDRLVNESDLPNLPYF 360
           ALLL++F+AGTDTSSSTTEWA+AELIRHPKILA VQQELD++VG+DRLV ESD+PNL YF
Sbjct: 301 ALLLDLFSAGTDTSSSTTEWAMAELIRHPKILAQVQQELDTVVGRDRLVTESDIPNLTYF 360

Query: 361 QAVMKEVFRLHPSTPLSLPRVSAEPCEINGYYIPKNTTLLVNVWAIARDPNVWADPLEFR 420
           QAV+KEVFRLHPSTPLSLPR++AE CEINGY+IPKNTTLLVNVWAIARDP+VWA+PLEFR
Sbjct: 361 QAVIKEVFRLHPSTPLSLPRMAAESCEINGYHIPKNTTLLVNVWAIARDPDVWANPLEFR 420

Query: 421 PERFLKGGERPNADVKGNDFEVIPFGAGRRICAGMSLGLRMVQLLTAALVHGFDWKLAHG 480
           PERFL GGE+PN DVKGNDFE+IPFGAGRRICAG+SLGLRMVQLLTA LVH ++WKLA G
Sbjct: 421 PERFLPGGEKPNVDVKGNDFELIPFGAGRRICAGLSLGLRMVQLLTATLVHSYEWKLADG 480

Query: 481 LAQEKLNMEEAYGLTLQRKVPLMVYPLPRLPAKAYTTT 519
           L  EKLNMEEAYGLTLQRK PLMV+P+ RL A AY  T
Sbjct: 481 LMVEKLNMEEAYGLTLQRKEPLMVHPVRRLSAHAYGPT 511

[Close]

BLAST of Spo05258.1 vs. NCBI nr
Match: gi|523496545|dbj|BAN62871.1| (flavonoid 3'-hydroxylase [Dianthus caryophyllus])

HSP 1 Score: 817.8 bits (2111), Expect = 1.100e-233
Identity = 405/519 (78.03%), Postives = 452/519 (87.09%), Query Frame = 1

		  
Query: 1   MSPLEIISYTIAIAIAIAIITYYLITTIIFRHPRPLPPGPKPWPIVGNLPHMGPVPHQGL 60
           MSPLE+  YTI +A     I YYLITT+   H +PLPPGP+PWPIVGNLPHMG  PHQGL
Sbjct: 1   MSPLELTFYTIVLAT----ILYYLITTVFRGHQKPLPPGPRPWPIVGNLPHMGQAPHQGL 60

Query: 61  AALAQKYGPLLYMRLGYVHVVVAASASVAAQFLKTHDANFSSRPPNSGAKHIAYNYQDMV 120
           AALAQKYGPLLYMRLGYV VVVAASASVA QFLKTHD NFSSRPPNSGAKHIAYNYQD+V
Sbjct: 61  AALAQKYGPLLYMRLGYVDVVVAASASVATQFLKTHDLNFSSRPPNSGAKHIAYNYQDLV 120

Query: 121 FAPYGQRWRLLRKISSLHLFSTKAMEDYKPIRQEEVGILTKALVKAESKEAVQLGQLLNV 180
           FAPYG +WR+LRKI SLH+FS+KA++D++ +RQEEV IL  A+ KA +K  VQLGQLLNV
Sbjct: 121 FAPYGPKWRMLRKICSLHMFSSKALDDFRLVRQEEVSILVNAIAKAGTKP-VQLGQLLNV 180

Query: 181 CTTNALSRVMLGKRVFGDGTGESDKKSEEFKDMVMELMVLAGEFNIGDFVPALEWLDLQG 240
           CTTNALSRVMLGKRV GDGTG+SD K+EEFKDMV+ELMVL G FNIGDFVPALE LDLQG
Sbjct: 181 CTTNALSRVMLGKRVLGDGTGKSDPKAEEFKDMVLELMVLTGVFNIGDFVPALECLDLQG 240

Query: 241 IATKMKKLHKRFDNFLGAILEEHKAKFKGGNFGGDFLSTLISLKDNCDGEGGKLTDIEIK 300
           +A+KMKKLHKR DNF+  ILEEHK+     N GGD LS LISLKDNCDGEGGK +D EIK
Sbjct: 241 VASKMKKLHKRLDNFMSNILEEHKSVAHQQN-GGDLLSILISLKDNCDGEGGKFSDTEIK 300

Query: 301 ALLLNMFTAGTDTSSSTTEWAIAELIRHPKILAHVQQELDSIVGQDRLVNESDLPNLPYF 360
           ALLL++FTAGTDTSSSTTEWAIAELIRHPKILA VQQE+DS+VG+DRL+ E+D+PNL YF
Sbjct: 301 ALLLDLFTAGTDTSSSTTEWAIAELIRHPKILAQVQQEMDSVVGRDRLIAEADIPNLTYF 360

Query: 361 QAVMKEVFRLHPSTPLSLPRVSAEPCEINGYYIPKNTTLLVNVWAIARDPNVWADPLEFR 420
           QAV+KEVFRLHPSTPLSLPRV+ E CEINGY+IPKNTTLLVNVWAIARDP VWADPLEF+
Sbjct: 361 QAVIKEVFRLHPSTPLSLPRVANESCEINGYHIPKNTTLLVNVWAIARDPEVWADPLEFK 420

Query: 421 PERFLKGGERPNADVKGNDFEVIPFGAGRRICAGMSLGLRMVQLLTAALVHGFDWKLAHG 480
           PERFL GGE+PN DVKGNDFE+IPFGAGRRICAG+SLGLRMVQL+TA L H +DW LA G
Sbjct: 421 PERFLPGGEKPNVDVKGNDFELIPFGAGRRICAGLSLGLRMVQLMTATLAHTYDWALADG 480

Query: 481 LAQEKLNMEEAYGLTLQRKVPLMVYPLPRLPAKAYTTTY 520
           L  EKLNM+EAYGLTLQRKVPLMV+P  RL A+ Y + +
Sbjct: 481 LMPEKLNMDEAYGLTLQRKVPLMVHPTRRLSARVYNSGF 513

[Close]

BLAST of Spo05258.1 vs. NCBI nr
Match: gi|470902337|dbj|BAN05297.1| (flavonoid 3'-hydroxylase [Dianthus caryophyllus])

HSP 1 Score: 813.5 bits (2100), Expect = 2.100e-232
Identity = 406/520 (78.08%), Postives = 452/520 (86.92%), Query Frame = 1

		  
Query: 1   MSPLEIISYTIAIAIAIAIITYYLITTIIFRHPRPLPPGPKPWPIVGNLPHMGPVPHQGL 60
           MSPLE+  YTIA+A     I YYLI T+   H +PLPPGP+PWPIVGNLPHMG  PHQGL
Sbjct: 1   MSPLELTFYTIALAT----ILYYLIPTVFRGHQKPLPPGPRPWPIVGNLPHMGQAPHQGL 60

Query: 61  AALAQKYGPLLYMRLGYVHVVVAASASVAAQFLKTHDANFSSRPPNSGAKHIAYNYQDMV 120
           AALAQKYGPLLYMRLGYV VVVAASASVA QFLKTHD NFSSRPPNSGAKHIAYNYQD+V
Sbjct: 61  AALAQKYGPLLYMRLGYVDVVVAASASVATQFLKTHDLNFSSRPPNSGAKHIAYNYQDLV 120

Query: 121 FAPYGQRWRLLRKISSLHLFSTKAMEDYKPIRQEEVGILTKALVKAESKEAVQLGQLL-N 180
           FAPYG +WR+LRKI SLH+FS+KA++D++ +RQEEV IL  A+ KA +K  VQLGQLL N
Sbjct: 121 FAPYGPKWRMLRKICSLHMFSSKALDDFRLVRQEEVSILVNAIAKAGTKP-VQLGQLLLN 180

Query: 181 VCTTNALSRVMLGKRVFGDGTGESDKKSEEFKDMVMELMVLAGEFNIGDFVPALEWLDLQ 240
           VCTTNALSRVMLGKRV GDGTG+SD K+EEFKDMV+ELMVL G FNIGDFVPALE LDLQ
Sbjct: 181 VCTTNALSRVMLGKRVLGDGTGKSDPKAEEFKDMVLELMVLTGVFNIGDFVPALECLDLQ 240

Query: 241 GIATKMKKLHKRFDNFLGAILEEHKAKFKGGNFGGDFLSTLISLKDNCDGEGGKLTDIEI 300
           G+A+KMKKLHKR DNF+  ILEEHK+     N GGD LSTLISLKDNCDGEGGK +D EI
Sbjct: 241 GVASKMKKLHKRLDNFMSNILEEHKSVAHQQN-GGDLLSTLISLKDNCDGEGGKFSDTEI 300

Query: 301 KALLLNMFTAGTDTSSSTTEWAIAELIRHPKILAHVQQELDSIVGQDRLVNESDLPNLPY 360
           KALLL++FTAGTDTSSSTTEWAIAELIRHPKILA VQQE+DS+VG+DRL+ E+D+PNL Y
Sbjct: 301 KALLLDLFTAGTDTSSSTTEWAIAELIRHPKILAQVQQEMDSVVGRDRLIAEADIPNLTY 360

Query: 361 FQAVMKEVFRLHPSTPLSLPRVSAEPCEINGYYIPKNTTLLVNVWAIARDPNVWADPLEF 420
           FQAV+KEVFRLHPSTPLSLPRV+ E CEINGY+IPKNTTLLVNVWAIARDP VWADPLEF
Sbjct: 361 FQAVIKEVFRLHPSTPLSLPRVANESCEINGYHIPKNTTLLVNVWAIARDPEVWADPLEF 420

Query: 421 RPERFLKGGERPNADVKGNDFEVIPFGAGRRICAGMSLGLRMVQLLTAALVHGFDWKLAH 480
           +PERFL GGE+PN DVKGNDFE+IPFGAGRRICAG+SLGLRMVQL+TA L H +DW LA 
Sbjct: 421 KPERFLPGGEKPNVDVKGNDFELIPFGAGRRICAGLSLGLRMVQLMTATLAHTYDWALAD 480

Query: 481 GLAQEKLNMEEAYGLTLQRKVPLMVYPLPRLPAKAYTTTY 520
           GL  EKLNM+EAYGLTLQRKVPLMV+P  RL A  Y + +
Sbjct: 481 GLMPEKLNMDEAYGLTLQRKVPLMVHPTRRLSAHVYNSGF 514

[Close]

BLAST of Spo05258.1 vs. NCBI nr
Match: gi|590668630|ref|XP_007037548.1| (Flavonoid 3'-hydroxylase isoform 2 [Theobroma cacao])

HSP 1 Score: 774.2 bits (1998), Expect = 1.400e-220
Identity = 380/503 (75.55%), Postives = 434/503 (86.28%), Query Frame = 1

		  
Query: 14  AIAIAIITYYLITTIIFRHPRPLPPGPKPWPIVGNLPHMGPVPHQGLAALAQKYGPLLYM 73
           +I  A+  Y++  T+  RH  PLPPGPKPWPI+GNLPHMGPVPH  LAALA+ YGPL+++
Sbjct: 8   SILSAVFLYFIFATLRKRHRLPLPPGPKPWPIIGNLPHMGPVPHHSLAALAKVYGPLMHL 67

Query: 74  RLGYVHVVVAASASVAAQFLKTHDANFSSRPPNSGAKHIAYNYQDMVFAPYGQRWRLLRK 133
           RLG+V VVVAASASVAAQFLK HDANFSSRPPNSGAK++AYNYQD+VFAPYG RWR+LRK
Sbjct: 68  RLGFVDVVVAASASVAAQFLKVHDANFSSRPPNSGAKYVAYNYQDLVFAPYGPRWRMLRK 127

Query: 134 ISSLHLFSTKAMEDYKPIRQEEVGILTKALVKAESKEAVQLGQLLNVCTTNALSRVMLGK 193
           ISS+HLFS KA++D++ +RQ+EVG+L +AL  A++K  V LGQLLNVCT NAL RVMLGK
Sbjct: 128 ISSVHLFSGKALDDFRHVRQDEVGVLVRALADAKTK--VNLGQLLNVCTVNALGRVMLGK 187

Query: 194 RVFGDGTGESDKKSEEFKDMVMELMVLAGEFNIGDFVPALEWLDLQGIATKMKKLHKRFD 253
           RVFGDG+G++D +++EFK MV+ELMVLAG  NIGDF+PALEWLDLQG+  KMKKLHKRFD
Sbjct: 188 RVFGDGSGKADPEADEFKSMVVELMVLAGVVNIGDFIPALEWLDLQGVQAKMKKLHKRFD 247

Query: 254 NFLGAILEEHKAKFK-GGNFGGDFLSTLISLKDNCDGEGGKLTDIEIKALLLNMFTAGTD 313
            FL AILEEHK K + G     D LST ISL+D  DGEGGKLTD EIKALLLNMFTAGTD
Sbjct: 248 RFLSAILEEHKIKARDGSGQHKDLLSTFISLED-ADGEGGKLTDTEIKALLLNMFTAGTD 307

Query: 314 TSSSTTEWAIAELIRHPKILAHVQQELDSIVGQDRLVNESDLPNLPYFQAVMKEVFRLHP 373
           TSSST EWAIAELIRHPKILA V++ELDS+VG+DRLV++ DLPNL YFQAV+KE FRLHP
Sbjct: 308 TSSSTVEWAIAELIRHPKILAQVRKELDSVVGRDRLVSDLDLPNLTYFQAVIKETFRLHP 367

Query: 374 STPLSLPRVSAEPCEINGYYIPKNTTLLVNVWAIARDPNVWADPLEFRPERFLKGGERPN 433
           STPLSLPR+++E CEINGY+IPK  TLLVNVWAIARDP+ W DPLEFRPERFL GGERPN
Sbjct: 368 STPLSLPRMASESCEINGYHIPKGATLLVNVWAIARDPDEWKDPLEFRPERFLPGGERPN 427

Query: 434 ADVKGNDFEVIPFGAGRRICAGMSLGLRMVQLLTAALVHGFDWKLAHGLAQEKLNMEEAY 493
           ADV+GNDFEVIPFGAGRRICAGMSLGLRMVQLL A LVH FDW+LA GL  EKLNMEEA+
Sbjct: 428 ADVRGNDFEVIPFGAGRRICAGMSLGLRMVQLLAATLVHAFDWELADGLMPEKLNMEEAF 487

Query: 494 GLTLQRKVPLMVYPLPRLPAKAY 516
           GLTLQR  PLMV+P PRL  +AY
Sbjct: 488 GLTLQRAAPLMVHPRPRLSPRAY 507

[Close]

BLAST of Spo05258.1 vs. UniProtKB/TrEMBL
Match: A0A142D8F1_9CARY (Flavonoid 3'hydroxylase OS=Silene littorea PE=2 SV=1)

HSP 1 Score: 824.7 bits (2129), Expect = 6.300e-236
Identity = 410/518 (79.15%), Postives = 455/518 (87.84%), Query Frame = 1

		  
Query: 1   MSPLEIISYTIAIAIAIAIITYYLITTIIFRHPRPLPPGPKPWPIVGNLPHMGPVPHQGL 60
           MS LE+  YTIAIA  +    YYL++T   R+P+PLPPGPKPWPIVGNLPHMGP PHQGL
Sbjct: 1   MSTLELTFYTIAIATLL----YYLLSTTFHRYPKPLPPGPKPWPIVGNLPHMGPAPHQGL 60

Query: 61  AALAQKYGPLLYMRLGYVHVVVAASASVAAQFLKTHDANFSSRPPNSGAKHIAYNYQDMV 120
           AALAQKYGPLLYMRLGYVHVVVAASASVA+QFLK HD NFSSRPPNSGAKHIAYNYQD+V
Sbjct: 61  AALAQKYGPLLYMRLGYVHVVVAASASVASQFLKVHDVNFSSRPPNSGAKHIAYNYQDLV 120

Query: 121 FAPYGQRWRLLRKISSLHLFSTKAMEDYKPIRQEEVGILTKALVKAESKEAVQLGQLLNV 180
           FAPYG +WR+LRKI S+H+FS KA++D   +RQEE  IL  AL  A++K  VQLGQLLNV
Sbjct: 121 FAPYGPKWRMLRKICSVHMFSNKALDDVTSVRQEEASILVNALANADAKP-VQLGQLLNV 180

Query: 181 CTTNALSRVMLGKRVFGDGTGESDKKSEEFKDMVMELMVLAGEFNIGDFVPALEWLDLQG 240
           CTTNALSRVMLG+RV GDGTG+SD K+EEFK+MVMELMVL G FNIGDFVP LE LDLQG
Sbjct: 181 CTTNALSRVMLGRRVLGDGTGKSDPKAEEFKEMVMELMVLTGVFNIGDFVPVLECLDLQG 240

Query: 241 IATKMKKLHKRFDNFLGAILEEHKAKFKGGNFGGDFLSTLISLKDNCDGEGGKLTDIEIK 300
           IA+KMK LHKR DNF+GAILEEHKA  K G  GGD LSTLISLKDN DGEGG+ TD EIK
Sbjct: 241 IASKMKNLHKRLDNFMGAILEEHKANGKHG--GGDLLSTLISLKDNYDGEGGQFTDTEIK 300

Query: 301 ALLLNMFTAGTDTSSSTTEWAIAELIRHPKILAHVQQELDSIVGQDRLVNESDLPNLPYF 360
           ALLL++F+AGTDTSSSTTEWA+AELIRHPKILA VQQELD++VG+DRLV ESD+PNL YF
Sbjct: 301 ALLLDLFSAGTDTSSSTTEWAMAELIRHPKILAQVQQELDTVVGRDRLVTESDIPNLTYF 360

Query: 361 QAVMKEVFRLHPSTPLSLPRVSAEPCEINGYYIPKNTTLLVNVWAIARDPNVWADPLEFR 420
           QAV+KEVFRLHPSTPLSLPR++AE CEINGY+IPKNTTLLVNVWAIARDP+VWA+PLEFR
Sbjct: 361 QAVIKEVFRLHPSTPLSLPRMAAESCEINGYHIPKNTTLLVNVWAIARDPDVWANPLEFR 420

Query: 421 PERFLKGGERPNADVKGNDFEVIPFGAGRRICAGMSLGLRMVQLLTAALVHGFDWKLAHG 480
           PERFL GGE+PN DVKGNDFE+IPFGAGRRICAG+SLGLRMVQLLTA LVH ++WKLA G
Sbjct: 421 PERFLPGGEKPNVDVKGNDFELIPFGAGRRICAGLSLGLRMVQLLTATLVHSYEWKLADG 480

Query: 481 LAQEKLNMEEAYGLTLQRKVPLMVYPLPRLPAKAYTTT 519
           L  EKLNMEEAYGLTLQRK PLMV+P+ RL A AY  T
Sbjct: 481 LMVEKLNMEEAYGLTLQRKEPLMVHPVRRLSAHAYGPT 511

[Close]

BLAST of Spo05258.1 vs. UniProtKB/TrEMBL
Match: S6B1I9_DIACA (Flavonoid 3'-hydroxylase OS=Dianthus caryophyllus GN=F3'H PE=3 SV=1)

HSP 1 Score: 817.8 bits (2111), Expect = 7.700e-234
Identity = 405/519 (78.03%), Postives = 452/519 (87.09%), Query Frame = 1

		  
Query: 1   MSPLEIISYTIAIAIAIAIITYYLITTIIFRHPRPLPPGPKPWPIVGNLPHMGPVPHQGL 60
           MSPLE+  YTI +A     I YYLITT+   H +PLPPGP+PWPIVGNLPHMG  PHQGL
Sbjct: 1   MSPLELTFYTIVLAT----ILYYLITTVFRGHQKPLPPGPRPWPIVGNLPHMGQAPHQGL 60

Query: 61  AALAQKYGPLLYMRLGYVHVVVAASASVAAQFLKTHDANFSSRPPNSGAKHIAYNYQDMV 120
           AALAQKYGPLLYMRLGYV VVVAASASVA QFLKTHD NFSSRPPNSGAKHIAYNYQD+V
Sbjct: 61  AALAQKYGPLLYMRLGYVDVVVAASASVATQFLKTHDLNFSSRPPNSGAKHIAYNYQDLV 120

Query: 121 FAPYGQRWRLLRKISSLHLFSTKAMEDYKPIRQEEVGILTKALVKAESKEAVQLGQLLNV 180
           FAPYG +WR+LRKI SLH+FS+KA++D++ +RQEEV IL  A+ KA +K  VQLGQLLNV
Sbjct: 121 FAPYGPKWRMLRKICSLHMFSSKALDDFRLVRQEEVSILVNAIAKAGTKP-VQLGQLLNV 180

Query: 181 CTTNALSRVMLGKRVFGDGTGESDKKSEEFKDMVMELMVLAGEFNIGDFVPALEWLDLQG 240
           CTTNALSRVMLGKRV GDGTG+SD K+EEFKDMV+ELMVL G FNIGDFVPALE LDLQG
Sbjct: 181 CTTNALSRVMLGKRVLGDGTGKSDPKAEEFKDMVLELMVLTGVFNIGDFVPALECLDLQG 240

Query: 241 IATKMKKLHKRFDNFLGAILEEHKAKFKGGNFGGDFLSTLISLKDNCDGEGGKLTDIEIK 300
           +A+KMKKLHKR DNF+  ILEEHK+     N GGD LS LISLKDNCDGEGGK +D EIK
Sbjct: 241 VASKMKKLHKRLDNFMSNILEEHKSVAHQQN-GGDLLSILISLKDNCDGEGGKFSDTEIK 300

Query: 301 ALLLNMFTAGTDTSSSTTEWAIAELIRHPKILAHVQQELDSIVGQDRLVNESDLPNLPYF 360
           ALLL++FTAGTDTSSSTTEWAIAELIRHPKILA VQQE+DS+VG+DRL+ E+D+PNL YF
Sbjct: 301 ALLLDLFTAGTDTSSSTTEWAIAELIRHPKILAQVQQEMDSVVGRDRLIAEADIPNLTYF 360

Query: 361 QAVMKEVFRLHPSTPLSLPRVSAEPCEINGYYIPKNTTLLVNVWAIARDPNVWADPLEFR 420
           QAV+KEVFRLHPSTPLSLPRV+ E CEINGY+IPKNTTLLVNVWAIARDP VWADPLEF+
Sbjct: 361 QAVIKEVFRLHPSTPLSLPRVANESCEINGYHIPKNTTLLVNVWAIARDPEVWADPLEFK 420

Query: 421 PERFLKGGERPNADVKGNDFEVIPFGAGRRICAGMSLGLRMVQLLTAALVHGFDWKLAHG 480
           PERFL GGE+PN DVKGNDFE+IPFGAGRRICAG+SLGLRMVQL+TA L H +DW LA G
Sbjct: 421 PERFLPGGEKPNVDVKGNDFELIPFGAGRRICAGLSLGLRMVQLMTATLAHTYDWALADG 480

Query: 481 LAQEKLNMEEAYGLTLQRKVPLMVYPLPRLPAKAYTTTY 520
           L  EKLNM+EAYGLTLQRKVPLMV+P  RL A+ Y + +
Sbjct: 481 LMPEKLNMDEAYGLTLQRKVPLMVHPTRRLSARVYNSGF 513

[Close]

BLAST of Spo05258.1 vs. UniProtKB/TrEMBL
Match: M5AAC5_DIACA (Flavonoid 3'-hydroxylase OS=Dianthus caryophyllus PE=3 SV=1)

HSP 1 Score: 813.5 bits (2100), Expect = 1.400e-232
Identity = 406/520 (78.08%), Postives = 452/520 (86.92%), Query Frame = 1

		  
Query: 1   MSPLEIISYTIAIAIAIAIITYYLITTIIFRHPRPLPPGPKPWPIVGNLPHMGPVPHQGL 60
           MSPLE+  YTIA+A     I YYLI T+   H +PLPPGP+PWPIVGNLPHMG  PHQGL
Sbjct: 1   MSPLELTFYTIALAT----ILYYLIPTVFRGHQKPLPPGPRPWPIVGNLPHMGQAPHQGL 60

Query: 61  AALAQKYGPLLYMRLGYVHVVVAASASVAAQFLKTHDANFSSRPPNSGAKHIAYNYQDMV 120
           AALAQKYGPLLYMRLGYV VVVAASASVA QFLKTHD NFSSRPPNSGAKHIAYNYQD+V
Sbjct: 61  AALAQKYGPLLYMRLGYVDVVVAASASVATQFLKTHDLNFSSRPPNSGAKHIAYNYQDLV 120

Query: 121 FAPYGQRWRLLRKISSLHLFSTKAMEDYKPIRQEEVGILTKALVKAESKEAVQLGQLL-N 180
           FAPYG +WR+LRKI SLH+FS+KA++D++ +RQEEV IL  A+ KA +K  VQLGQLL N
Sbjct: 121 FAPYGPKWRMLRKICSLHMFSSKALDDFRLVRQEEVSILVNAIAKAGTKP-VQLGQLLLN 180

Query: 181 VCTTNALSRVMLGKRVFGDGTGESDKKSEEFKDMVMELMVLAGEFNIGDFVPALEWLDLQ 240
           VCTTNALSRVMLGKRV GDGTG+SD K+EEFKDMV+ELMVL G FNIGDFVPALE LDLQ
Sbjct: 181 VCTTNALSRVMLGKRVLGDGTGKSDPKAEEFKDMVLELMVLTGVFNIGDFVPALECLDLQ 240

Query: 241 GIATKMKKLHKRFDNFLGAILEEHKAKFKGGNFGGDFLSTLISLKDNCDGEGGKLTDIEI 300
           G+A+KMKKLHKR DNF+  ILEEHK+     N GGD LSTLISLKDNCDGEGGK +D EI
Sbjct: 241 GVASKMKKLHKRLDNFMSNILEEHKSVAHQQN-GGDLLSTLISLKDNCDGEGGKFSDTEI 300

Query: 301 KALLLNMFTAGTDTSSSTTEWAIAELIRHPKILAHVQQELDSIVGQDRLVNESDLPNLPY 360
           KALLL++FTAGTDTSSSTTEWAIAELIRHPKILA VQQE+DS+VG+DRL+ E+D+PNL Y
Sbjct: 301 KALLLDLFTAGTDTSSSTTEWAIAELIRHPKILAQVQQEMDSVVGRDRLIAEADIPNLTY 360

Query: 361 FQAVMKEVFRLHPSTPLSLPRVSAEPCEINGYYIPKNTTLLVNVWAIARDPNVWADPLEF 420
           FQAV+KEVFRLHPSTPLSLPRV+ E CEINGY+IPKNTTLLVNVWAIARDP VWADPLEF
Sbjct: 361 FQAVIKEVFRLHPSTPLSLPRVANESCEINGYHIPKNTTLLVNVWAIARDPEVWADPLEF 420

Query: 421 RPERFLKGGERPNADVKGNDFEVIPFGAGRRICAGMSLGLRMVQLLTAALVHGFDWKLAH 480
           +PERFL GGE+PN DVKGNDFE+IPFGAGRRICAG+SLGLRMVQL+TA L H +DW LA 
Sbjct: 421 KPERFLPGGEKPNVDVKGNDFELIPFGAGRRICAGLSLGLRMVQLMTATLAHTYDWALAD 480

Query: 481 GLAQEKLNMEEAYGLTLQRKVPLMVYPLPRLPAKAYTTTY 520
           GL  EKLNM+EAYGLTLQRKVPLMV+P  RL A  Y + +
Sbjct: 481 GLMPEKLNMDEAYGLTLQRKVPLMVHPTRRLSAHVYNSGF 514

[Close]

BLAST of Spo05258.1 vs. UniProtKB/TrEMBL
Match: A0A061G4T4_THECC (Flavonoid 3'-hydroxylase isoform 2 OS=Theobroma cacao GN=TCM_014241 PE=3 SV=1)

HSP 1 Score: 774.2 bits (1998), Expect = 9.700e-221
Identity = 380/503 (75.55%), Postives = 434/503 (86.28%), Query Frame = 1

		  
Query: 14  AIAIAIITYYLITTIIFRHPRPLPPGPKPWPIVGNLPHMGPVPHQGLAALAQKYGPLLYM 73
           +I  A+  Y++  T+  RH  PLPPGPKPWPI+GNLPHMGPVPH  LAALA+ YGPL+++
Sbjct: 8   SILSAVFLYFIFATLRKRHRLPLPPGPKPWPIIGNLPHMGPVPHHSLAALAKVYGPLMHL 67

Query: 74  RLGYVHVVVAASASVAAQFLKTHDANFSSRPPNSGAKHIAYNYQDMVFAPYGQRWRLLRK 133
           RLG+V VVVAASASVAAQFLK HDANFSSRPPNSGAK++AYNYQD+VFAPYG RWR+LRK
Sbjct: 68  RLGFVDVVVAASASVAAQFLKVHDANFSSRPPNSGAKYVAYNYQDLVFAPYGPRWRMLRK 127

Query: 134 ISSLHLFSTKAMEDYKPIRQEEVGILTKALVKAESKEAVQLGQLLNVCTTNALSRVMLGK 193
           ISS+HLFS KA++D++ +RQ+EVG+L +AL  A++K  V LGQLLNVCT NAL RVMLGK
Sbjct: 128 ISSVHLFSGKALDDFRHVRQDEVGVLVRALADAKTK--VNLGQLLNVCTVNALGRVMLGK 187

Query: 194 RVFGDGTGESDKKSEEFKDMVMELMVLAGEFNIGDFVPALEWLDLQGIATKMKKLHKRFD 253
           RVFGDG+G++D +++EFK MV+ELMVLAG  NIGDF+PALEWLDLQG+  KMKKLHKRFD
Sbjct: 188 RVFGDGSGKADPEADEFKSMVVELMVLAGVVNIGDFIPALEWLDLQGVQAKMKKLHKRFD 247

Query: 254 NFLGAILEEHKAKFK-GGNFGGDFLSTLISLKDNCDGEGGKLTDIEIKALLLNMFTAGTD 313
            FL AILEEHK K + G     D LST ISL+D  DGEGGKLTD EIKALLLNMFTAGTD
Sbjct: 248 RFLSAILEEHKIKARDGSGQHKDLLSTFISLED-ADGEGGKLTDTEIKALLLNMFTAGTD 307

Query: 314 TSSSTTEWAIAELIRHPKILAHVQQELDSIVGQDRLVNESDLPNLPYFQAVMKEVFRLHP 373
           TSSST EWAIAELIRHPKILA V++ELDS+VG+DRLV++ DLPNL YFQAV+KE FRLHP
Sbjct: 308 TSSSTVEWAIAELIRHPKILAQVRKELDSVVGRDRLVSDLDLPNLTYFQAVIKETFRLHP 367

Query: 374 STPLSLPRVSAEPCEINGYYIPKNTTLLVNVWAIARDPNVWADPLEFRPERFLKGGERPN 433
           STPLSLPR+++E CEINGY+IPK  TLLVNVWAIARDP+ W DPLEFRPERFL GGERPN
Sbjct: 368 STPLSLPRMASESCEINGYHIPKGATLLVNVWAIARDPDEWKDPLEFRPERFLPGGERPN 427

Query: 434 ADVKGNDFEVIPFGAGRRICAGMSLGLRMVQLLTAALVHGFDWKLAHGLAQEKLNMEEAY 493
           ADV+GNDFEVIPFGAGRRICAGMSLGLRMVQLL A LVH FDW+LA GL  EKLNMEEA+
Sbjct: 428 ADVRGNDFEVIPFGAGRRICAGMSLGLRMVQLLAATLVHAFDWELADGLMPEKLNMEEAF 487

Query: 494 GLTLQRKVPLMVYPLPRLPAKAY 516
           GLTLQR  PLMV+P PRL  +AY
Sbjct: 488 GLTLQRAAPLMVHPRPRLSPRAY 507

[Close]

BLAST of Spo05258.1 vs. UniProtKB/TrEMBL
Match: A0A0H3WMD5_CAMSI (Flavonoid 3'-hydroxylase 2 OS=Camellia sinensis GN=F3'-H2 PE=2 SV=1)

HSP 1 Score: 768.5 bits (1983), Expect = 5.300e-219
Identity = 377/517 (72.92%), Postives = 434/517 (83.95%), Query Frame = 1

		  
Query: 1   MSPLEIISYTIAIAIAIAIITYYLITTIIFRHPRPLPPGPKPWPIVGNLPHMGPVPHQGL 60
           M+ L  + Y++ IA+ +  +   L T +  RHPRPLPPGP PWP++GNLPH+G +PH  +
Sbjct: 1   MTSLAFVLYSLLIAVFLYCLLN-LRTLLFNRHPRPLPPGPTPWPVIGNLPHLGTMPHHSI 60

Query: 61  AALAQKYGPLLYMRLGYVHVVVAASASVAAQFLKTHDANFSSRPPNSGAKHIAYNYQDMV 120
           A +A+ YGPL+++RLG V VVVAASASVAAQFLKTHDANFSSRPPNSGAKHIAYNYQD+V
Sbjct: 61  ATMARTYGPLMHLRLGLVDVVVAASASVAAQFLKTHDANFSSRPPNSGAKHIAYNYQDLV 120

Query: 121 FAPYGQRWRLLRKISSLHLFSTKAMEDYKPIRQEEVGILTKALVKAESKEAVQLGQLLNV 180
           FAPYG RWR+LRKI S+HLFSTKA++D++ +RQEE+ ILT+AL  A    AV LGQLLNV
Sbjct: 121 FAPYGPRWRMLRKICSVHLFSTKALDDFRHVRQEEIAILTRALESAGQAAAVNLGQLLNV 180

Query: 181 CTTNALSRVMLGKRVFGDGTGESDKKSEEFKDMVMELMVLAGEFNIGDFVPALEWLDLQG 240
           CTTNAL RVMLG RVFGDG+G  D K++EFK+MV+ELMVLAG FNIGDFVPALEWLDLQG
Sbjct: 181 CTTNALGRVMLGHRVFGDGSGSGDPKADEFKEMVVELMVLAGVFNIGDFVPALEWLDLQG 240

Query: 241 IATKMKKLHKRFDNFLGAILEEHKAKFK--GGNFGGDFLSTLISLKDNCDGEGGKLTDIE 300
           +A+KMKKLH RFD+FL  ILEEHK  +   G     D LSTLISLKD+ DGEGGKLTDIE
Sbjct: 241 VASKMKKLHARFDSFLNKILEEHKVNYSSSGAQKHTDLLSTLISLKDDADGEGGKLTDIE 300

Query: 301 IKALLLNMFTAGTDTSSSTTEWAIAELIRHPKILAHVQQELDSIVGQDRLVNESDLPNLP 360
           IKALLL++FTAGTDTSSST EWAIAELIRHPKILA  +QELDSIVG DR V ESDL  L 
Sbjct: 301 IKALLLDLFTAGTDTSSSTVEWAIAELIRHPKILAQAKQELDSIVGPDRRVTESDLAQLT 360

Query: 361 YFQAVMKEVFRLHPSTPLSLPRVSAEPCEINGYYIPKNTTLLVNVWAIARDPNVWADPLE 420
           + QA++KE FRLHPSTPLSLPR++++ CEINGY+IPK +TLLVNVWAIARDP+ WA+PLE
Sbjct: 361 FLQAIIKETFRLHPSTPLSLPRMASDSCEINGYFIPKGSTLLVNVWAIARDPDAWAEPLE 420

Query: 421 FRPERFLKGGERPNADVKGNDFEVIPFGAGRRICAGMSLGLRMVQLLTAALVHGFDWKLA 480
           FRPERFL GGE+PNADV+GNDFEVIPFGAGRRICAGMSLGLRMVQLLTA LVH FDW LA
Sbjct: 421 FRPERFLPGGEKPNADVRGNDFEVIPFGAGRRICAGMSLGLRMVQLLTATLVHAFDWDLA 480

Query: 481 HGLAQEKLNMEEAYGLTLQRKVPLMVYPLPRLPAKAY 516
            G + EKLNM+EAYGLTLQR  PLMV+P PRL    Y
Sbjct: 481 DGQSTEKLNMDEAYGLTLQRAAPLMVHPRPRLAPHVY 516

[Close]

BLAST of Spo05258.1 vs. ExPASy Swiss-Prot
Match: F3PH_PETHY (Flavonoid 3'-monooxygenase OS=Petunia hybrida GN=CYP75B2 PE=2 SV=1)

HSP 1 Score: 724.9 bits (1870), Expect = 6.100e-208
Identity = 356/513 (69.40%), Postives = 422/513 (82.26%), Query Frame = 1

		  
Query: 4   LEIISYTIAIAIAIAIITYYLITTIIFRHPRPLPPGPKPWPIVGNLPHMGPVPHQGLAAL 63
           +EI+S  +   I   ++ + L +    R+P PLPPGPKPWPI+GNL H+GP PHQ  AA+
Sbjct: 1   MEILSLILYTVIFSFLLQFILRSFFRKRYPLPLPPGPKPWPIIGNLVHLGPKPHQSTAAM 60

Query: 64  AQKYGPLLYMRLGYVHVVVAASASVAAQFLKTHDANFSSRPPNSGAKHIAYNYQDMVFAP 123
           AQ YGPL+Y+++G+V VVVAASASVAAQFLKTHDANFSSRPPNSGA+H+AYNYQD+VFAP
Sbjct: 61  AQTYGPLMYLKMGFVDVVVAASASVAAQFLKTHDANFSSRPPNSGAEHMAYNYQDLVFAP 120

Query: 124 YGQRWRLLRKISSLHLFSTKAMEDYKPIRQEEVGILTKALVKAESKEAVQLGQLLNVCTT 183
           YG RWR+LRKI S+HLFSTKA++D++ +RQ+EV  LT+AL  A  K  V+LGQLLNVCTT
Sbjct: 121 YGPRWRMLRKICSVHLFSTKALDDFRHVRQDEVKTLTRALASAGQKP-VKLGQLLNVCTT 180

Query: 184 NALSRVMLGKRVFGDGTGESDKKSEEFKDMVMELMVLAGEFNIGDFVPALEWLDLQGIAT 243
           NAL+RVMLGKRVF DG+G+ D ++ EFK MV+E+MV+AG FNIGDF+P L WLD+QG+A 
Sbjct: 181 NALARVMLGKRVFADGSGDVDPQAAEFKSMVVEMMVVAGVFNIGDFIPQLNWLDIQGVAA 240

Query: 244 KMKKLHKRFDNFLGAILEEHKAKFKGGNFGGDFLSTLISLK-DNCDGEGGKLTDIEIKAL 303
           KMKKLH RFD FL  ILEEHK K  G     D LSTLISLK D+ D +GGKLTD EIKAL
Sbjct: 241 KMKKLHARFDAFLTDILEEHKGKIFGEM--KDLLSTLISLKNDDADNDGGKLTDTEIKAL 300

Query: 304 LLNMFTAGTDTSSSTTEWAIAELIRHPKILAHVQQELDSIVGQDRLVNESDLPNLPYFQA 363
           LLN+F AGTDTSSST EWAIAELIR+PKILA  QQE+D +VG+DRLV E DL  L Y +A
Sbjct: 301 LLNLFVAGTDTSSSTVEWAIAELIRNPKILAQAQQEIDKVVGRDRLVGELDLAQLTYLEA 360

Query: 364 VMKEVFRLHPSTPLSLPRVSAEPCEINGYYIPKNTTLLVNVWAIARDPNVWADPLEFRPE 423
           ++KE FRLHPSTPLSLPR+++E CEINGY+IPK +TLL+NVWAIARDPN WADPLEFRPE
Sbjct: 361 IVKETFRLHPSTPLSLPRIASESCEINGYFIPKGSTLLLNVWAIARDPNAWADPLEFRPE 420

Query: 424 RFLKGGERPNADVKGNDFEVIPFGAGRRICAGMSLGLRMVQLLTAALVHGFDWKLAHGLA 483
           RFL GGE+P  DV+GNDFEVIPFGAGRRICAGM+LG+RMVQL+ A L+H F+W L  G  
Sbjct: 421 RFLPGGEKPKVDVRGNDFEVIPFGAGRRICAGMNLGIRMVQLMIATLIHAFNWDLVSGQL 480

Query: 484 QEKLNMEEAYGLTLQRKVPLMVYPLPRLPAKAY 516
            E LNMEEAYGLTLQR  PL+V+P PRL A+AY
Sbjct: 481 PEMLNMEEAYGLTLQRADPLVVHPRPRLEAQAY 510

[Close]

BLAST of Spo05258.1 vs. ExPASy Swiss-Prot
Match: F3PH_ARATH (Flavonoid 3'-monooxygenase OS=Arabidopsis thaliana GN=CYP75B1 PE=1 SV=1)

HSP 1 Score: 677.9 bits (1748), Expect = 8.500e-194
Identity = 333/511 (65.17%), Postives = 406/511 (79.45%), Query Frame = 1

		  
Query: 10  TIAIAIAIAIITYYLITTIIFR----HPRPLPPGPKPWPIVGNLPHMGPVPHQGLAALAQ 69
           T+ + I +A + + ++     R    H   LPPGP PWPI+GNLPHMG  PH+ L+A+  
Sbjct: 3   TLFLTILLATVLFLILRIFSHRRNRSHNNRLPPGPNPWPIIGNLPHMGTKPHRTLSAMVT 62

Query: 70  KYGPLLYMRLGYVHVVVAASASVAAQFLKTHDANFSSRPPNSGAKHIAYNYQDMVFAPYG 129
            YGP+L++RLG+V VVVAAS SVA QFLK HDANF+SRPPNSGAKH+AYNYQD+VFAPYG
Sbjct: 63  TYGPILHLRLGFVDVVVAASKSVAEQFLKIHDANFASRPPNSGAKHMAYNYQDLVFAPYG 122

Query: 130 QRWRLLRKISSLHLFSTKAMEDYKPIRQEEVGILTKALVKAESKEAVQLGQLLNVCTTNA 189
            RWRLLRKISS+HLFS KA+ED+K +RQEEVG LT+ LV+  +K  V LGQL+N+C  NA
Sbjct: 123 HRWRLLRKISSVHLFSAKALEDFKHVRQEEVGTLTRELVRVGTKP-VNLGQLVNMCVVNA 182

Query: 190 LSRVMLGKRVFGDGTGESDKKSEEFKDMVMELMVLAGEFNIGDFVPALEWLDLQGIATKM 249
           L R M+G+R+FG    ++D K++EF+ MV E+M LAG FNIGDFVP+L+WLDLQG+A KM
Sbjct: 183 LGREMIGRRLFG---ADADHKADEFRSMVTEMMALAGVFNIGDFVPSLDWLDLQGVAGKM 242

Query: 250 KKLHKRFDNFLGAILEEHKAKFKGGNFGGDFLSTLISLKD-NCDGEGGKLTDIEIKALLL 309
           K+LHKRFD FL +IL+EH+   +      D LSTLISLK  + DG+GG LTD EIKALLL
Sbjct: 243 KRLHKRFDAFLSSILKEHEMNGQDQKHT-DMLSTLISLKGTDLDGDGGSLTDTEIKALLL 302

Query: 310 NMFTAGTDTSSSTTEWAIAELIRHPKILAHVQQELDSIVGQDRLVNESDLPNLPYFQAVM 369
           NMFTAGTDTS+ST +WAIAELIRHP I+   Q+ELD +VG+DR VNESD+  LPY QAV+
Sbjct: 303 NMFTAGTDTSASTVDWAIAELIRHPDIMVKAQEELDIVVGRDRPVNESDIAQLPYLQAVI 362

Query: 370 KEVFRLHPSTPLSLPRVSAEPCEINGYYIPKNTTLLVNVWAIARDPNVWADPLEFRPERF 429
           KE FRLHP TPLSLP +++E CEINGY+IPK +TLL N+WAIARDP+ W+DPL F+PERF
Sbjct: 363 KENFRLHPPTPLSLPHIASESCEINGYHIPKGSTLLTNIWAIARDPDQWSDPLAFKPERF 422

Query: 430 LKGGERPNADVKGNDFEVIPFGAGRRICAGMSLGLRMVQLLTAALVHGFDWKLAHGLAQE 489
           L GGE+   DVKG+DFE+IPFGAGRRICAG+SLGLR +Q LTA LV GFDW+LA G+  E
Sbjct: 423 LPGGEKSGVDVKGSDFELIPFGAGRRICAGLSLGLRTIQFLTATLVQGFDWELAGGVTPE 482

Query: 490 KLNMEEAYGLTLQRKVPLMVYPLPRLPAKAY 516
           KLNMEE+YGLTLQR VPL+V+P PRL    Y
Sbjct: 483 KLNMEESYGLTLQRAVPLVVHPKPRLAPNVY 508

[Close]

BLAST of Spo05258.1 vs. ExPASy Swiss-Prot
Match: C75A1_PETHY (Flavonoid 3',5'-hydroxylase 1 OS=Petunia hybrida GN=CYP75A1 PE=2 SV=1)

HSP 1 Score: 521.2 bits (1341), Expect = 1.300e-146
Identity = 261/507 (51.48%), Postives = 352/507 (69.43%), Query Frame = 1

		  
Query: 11  IAIAIAIAIITYYLITTIIFRHP-RPLPPGPKPWPIVGNLPHMGPVPHQGLAALAQKYGP 70
           +  A +I +I + +I+T+I +   R LPPGP+ WP++G LP +G +PH  LA +A+KYG 
Sbjct: 7   LGAATSIFLIAHIIISTLISKTTGRHLPPGPRGWPVIGALPLLGAMPHVSLAKMAKKYGA 66

Query: 71  LLYMRLGYVHVVVAASASVAAQFLKTHDANFSSRPPNSGAKHIAYNYQDMVFAPYGQRWR 130
           ++Y+++G   + VA++   A  FLKT D NFS+RPPN+GA H+AYN QDMVFA YG RW+
Sbjct: 67  IMYLKVGTCGMAVASTPDAAKAFLKTLDINFSNRPPNAGATHLAYNAQDMVFAHYGPRWK 126

Query: 131 LLRKISSLHLFSTKAMEDYKPIRQEEVGILTKALVKAESK-EAVQLGQLLNVCTTNALSR 190
           LLRK+S+LH+   KA+E++  +R  E+G + K++     + + V + ++L     N + +
Sbjct: 127 LLRKLSNLHMLGGKALENWANVRANELGHMLKSMSDMSREGQRVVVAEMLTFAMANMIGQ 186

Query: 191 VMLGKRVFGDGTGESDKKSEEFKDMVMELMVLAGEFNIGDFVPALEWLDLQGIATKMKKL 250
           VML KRVF D   E +    EFKDMV+ELM +AG FNIGDF+P L W+DLQGI  +MK+L
Sbjct: 187 VMLSKRVFVDKGVEVN----EFKDMVVELMTIAGYFNIGDFIPCLAWMDLQGIEKRMKRL 246

Query: 251 HKRFDNFLGAILEEHKAKFKGGNFGGDFLSTLISLKDNCDGEGGKLTDIEIKALLLNMFT 310
           HK+FD  L  + +EHKA         DFL  ++   DN +GE  +L+   IKALLLN+FT
Sbjct: 247 HKKFDALLTKMFDEHKATTYERKGKPDFLDVVMENGDNSEGE--RLSTTNIKALLLNLFT 306

Query: 311 AGTDTSSSTTEWAIAELIRHPKILAHVQQELDSIVGQDRLVNESDLPNLPYFQAVMKEVF 370
           AGTDTSSS  EWA+AE++++P IL   Q E+D ++G++R + ESD+PNLPY +A+ KE F
Sbjct: 307 AGTDTSSSAIEWALAEMMKNPAILKKAQAEMDQVIGRNRRLLESDIPNLPYLRAICKETF 366

Query: 371 RLHPSTPLSLPRVSAEPCEINGYYIPKNTTLLVNVWAIARDPNVWADPLEFRPERFLKGG 430
           R HPSTPL+LPR+S EPC ++GYYIPKNT L VN+WAI RDP VW +PLEF PERFL G 
Sbjct: 367 RKHPSTPLNLPRISNEPCIVDGYYIPKNTRLSVNIWAIGRDPQVWENPLEFNPERFLSG- 426

Query: 431 ERPNADVKGNDFEVIPFGAGRRICAGMSLGLRMVQLLTAALVHGFDWKLAHGLAQEKLNM 490
                D +GNDFE+IPFGAGRRICAG  +G+ MV+ +   LVH FDWKL   + +  LNM
Sbjct: 427 RNSKIDPRGNDFELIPFGAGRRICAGTRMGIVMVEYILGTLVHSFDWKLPSEVIE--LNM 486

Query: 491 EEAYGLTLQRKVPLMVYPLPRLPAKAY 516
           EEA+GL LQ+ VPL     PRL    Y
Sbjct: 487 EEAFGLALQKAVPLEAMVTPRLQLDVY 504

[Close]

BLAST of Spo05258.1 vs. ExPASy Swiss-Prot
Match: C75A2_SOLME (Flavonoid 3',5'-hydroxylase OS=Solanum melongena GN=CYP75A2 PE=2 SV=1)

HSP 1 Score: 520.4 bits (1339), Expect = 2.300e-146
Identity = 266/517 (51.45%), Postives = 359/517 (69.44%), Query Frame = 1

		  
Query: 3   PLEIISYTIAIAIAIAIITYYLITTIIFRHPRPLPPGPKPWPIVGNLPHMGPVPHQGLAA 62
           P E+I  TI I I + II   LI T  +R  R LPPGP+ WP++G LP +G +PH  LA 
Sbjct: 5   PSELIGATI-IYIIVYIIIQKLIATGSWRRRR-LPPGPEGWPVIGALPLLGGMPHVALAK 64

Query: 63  LAQKYGPLLYMRLGYVHVVVAASASVAAQFLKTHDANFSSRPPNSGAKHIAYNYQDMVFA 122
           +A+KYGP++Y+++G   +VVA++ + A  FLKT D NFS+RPPN+GA H+AYN QDMVFA
Sbjct: 65  MAKKYGPIMYLKVGTCGMVVASTPNAAKAFLKTLDINFSNRPPNAGATHMAYNAQDMVFA 124

Query: 123 PYGQRWRLLRKISSLHLFSTKAMEDYKPIRQEEVGILTKALVKAESK-EAVQLGQLLNVC 182
           PYG RW+LLRK+S+LH+   KA+E++  +R  E+G + K++  A    E + +  +L   
Sbjct: 125 PYGPRWKLLRKLSNLHMLGGKALENWANVRANELGHMLKSMFDASHVGERIVVADMLTFA 184

Query: 183 TTNALSRVMLGKRVFGDGTGESDKKSEEFKDMVMELMVLAGEFNIGDFVPALEWLDLQGI 242
             N + +VML KRVF     E  K+  EFK+MV+ELM +AG FNIGDF+P + W+DLQGI
Sbjct: 185 MANMIGQVMLSKRVFV----EKGKEVNEFKNMVVELMTVAGYFNIGDFIPQIAWMDLQGI 244

Query: 243 ATKMKKLHKRFDNFLGAILEEHKAKFKGGNFGGDFLSTLISLKDNCDGEGGKLTDIEIKA 302
              MKKLHK+FD+ L  + EEH+A         DFL  +++ +DN +GE  +L+   IKA
Sbjct: 245 EKGMKKLHKKFDDLLTKMFEEHEATSNERKGKPDFLDFIMANRDNSEGE--RLSITNIKA 304

Query: 303 LLLNMFTAGTDTSSSTTEWAIAELIRHPKILAHVQQELDSIVGQDRLVNESDLPNLPYFQ 362
           LLLN+FTAGTDTSSS  EWA+ E++++P I    QQE+D I+G++R   ESD+PNLPY +
Sbjct: 305 LLLNLFTAGTDTSSSVIEWALTEMMKNPTIFKKAQQEMDQIIGKNRRFIESDIPNLPYLR 364

Query: 363 AVMKEVFRLHPSTPLSLPRVSAEPCEINGYYIPKNTTLLVNVWAIARDPNVWADPLEFRP 422
           A+ KE FR HPSTPL+LPRVS++ C I+GYYIPKNT L VN+WAI RDP+VW +PLEF P
Sbjct: 365 AICKEAFRKHPSTPLNLPRVSSDACTIDGYYIPKNTRLSVNIWAIGRDPDVWENPLEFIP 424

Query: 423 ERFLKGGERPNADVKGNDFEVIPFGAGRRICAGMSLGLRMVQLLTAALVHGFDWKLAHGL 482
           ERFL   +    + +GNDFE+IPFGAGRRICAG  +G+ MV+ +   L+H FDWKL + +
Sbjct: 425 ERFL-SEKNAKIEHRGNDFELIPFGAGRRICAGTRMGIVMVEYILGTLIHSFDWKLPNDV 484

Query: 483 AQEKLNMEEAYGLTLQRKVPLMVYPLPRLPAKAYTTT 519
               +NMEE +GL LQ+ VPL     PRL    Y ++
Sbjct: 485 VD--INMEETFGLALQKAVPLEAIVTPRLSFDIYQSS 510

[Close]

BLAST of Spo05258.1 vs. ExPASy Swiss-Prot
Match: C75A3_PETHY (Flavonoid 3',5'-hydroxylase 2 OS=Petunia hybrida GN=CYP75A3 PE=2 SV=1)

HSP 1 Score: 518.1 bits (1333), Expect = 1.100e-145
Identity = 261/507 (51.48%), Postives = 350/507 (69.03%), Query Frame = 1

		  
Query: 11  IAIAIAIAIITYYLITTII-FRHPRPLPPGPKPWPIVGNLPHMGPVPHQGLAALAQKYGP 70
           +A A  I + T+  I+T++   + R LPPGP+ WP++G LP +G +PH  LA +A+KYG 
Sbjct: 7   LAAATLIFLTTHIFISTLLSITNGRRLPPGPRGWPVIGALPLLGAMPHVSLAKMAKKYGA 66

Query: 71  LLYMRLGYVHVVVAASASVAAQFLKTHDANFSSRPPNSGAKHIAYNYQDMVFAPYGQRWR 130
           ++Y+++G   +VVA++   A  FLKT D NFS+RPPN+GA H+AY  QDMVFA YG RW+
Sbjct: 67  IMYLKVGTCGMVVASTPDAAKAFLKTLDLNFSNRPPNAGATHLAYGAQDMVFAHYGPRWK 126

Query: 131 LLRKISSLHLFSTKAMEDYKPIRQEEVGILTKALVKAESK-EAVQLGQLLNVCTTNALSR 190
           LLRK+S+LH+   KA+E++  +R  E+G + K++     + E V + ++L     N + +
Sbjct: 127 LLRKLSNLHMLGGKALENWANVRANELGHMLKSMFDMSREGERVVVAEMLTFAMANMIGQ 186

Query: 191 VMLGKRVFGDGTGESDKKSEEFKDMVMELMVLAGEFNIGDFVPALEWLDLQGIATKMKKL 250
           V+L KRVF +   E +    EFKDMV+ELM  AG FNIGDF+P L W+DLQGI   MK+L
Sbjct: 187 VILSKRVFVNKGVEVN----EFKDMVVELMTTAGYFNIGDFIPCLAWMDLQGIEKGMKRL 246

Query: 251 HKRFDNFLGAILEEHKAKFKGGNFGGDFLSTLISLKDNCDGEGGKLTDIEIKALLLNMFT 310
           HK+FD  L  + +EHKA         DFL  ++  +DN +GE  +L+   IKALLLN+FT
Sbjct: 247 HKKFDALLTKMFDEHKATSYERKGKPDFLDCVMENRDNSEGE--RLSTTNIKALLLNLFT 306

Query: 311 AGTDTSSSTTEWAIAELIRHPKILAHVQQELDSIVGQDRLVNESDLPNLPYFQAVMKEVF 370
           AGTDTSSS  EWA+AE++++P IL   Q E+D ++G +R + ESD+PNLPY +A+ KE F
Sbjct: 307 AGTDTSSSAIEWALAEMMKNPAILKKAQGEMDQVIGNNRRLLESDIPNLPYLRAICKETF 366

Query: 371 RLHPSTPLSLPRVSAEPCEINGYYIPKNTTLLVNVWAIARDPNVWADPLEFRPERFLKGG 430
           R HPSTPL+LPR+S EPC ++GYYIPKNT L VN+WAI RDP VW +PLEF PERFL G 
Sbjct: 367 RKHPSTPLNLPRISNEPCIVDGYYIPKNTRLSVNIWAIGRDPEVWENPLEFYPERFLSG- 426

Query: 431 ERPNADVKGNDFEVIPFGAGRRICAGMSLGLRMVQLLTAALVHGFDWKLAHGLAQEKLNM 490
                D +GNDFE+IPFGAGRRICAG  +G+ MV+ +   LVH FDWKL   + +  LNM
Sbjct: 427 RNSKIDPRGNDFELIPFGAGRRICAGTRMGIVMVEYILGTLVHSFDWKLPSEVIE--LNM 486

Query: 491 EEAYGLTLQRKVPLMVYPLPRLPAKAY 516
           EEA+GL LQ+ VPL     PRLP   Y
Sbjct: 487 EEAFGLALQKAVPLEAMVTPRLPIDVY 504

[Close]

BLAST of Spo05258.1 vs. TAIR (Arabidopsis)
Match: AT5G07990.1 (Cytochrome P450 superfamily protein)

HSP 1 Score: 677.9 bits (1748), Expect = 4.800e-195
Identity = 333/511 (65.17%), Postives = 406/511 (79.45%), Query Frame = 1

		  
Query: 10  TIAIAIAIAIITYYLITTIIFR----HPRPLPPGPKPWPIVGNLPHMGPVPHQGLAALAQ 69
           T+ + I +A + + ++     R    H   LPPGP PWPI+GNLPHMG  PH+ L+A+  
Sbjct: 3   TLFLTILLATVLFLILRIFSHRRNRSHNNRLPPGPNPWPIIGNLPHMGTKPHRTLSAMVT 62

Query: 70  KYGPLLYMRLGYVHVVVAASASVAAQFLKTHDANFSSRPPNSGAKHIAYNYQDMVFAPYG 129
            YGP+L++RLG+V VVVAAS SVA QFLK HDANF+SRPPNSGAKH+AYNYQD+VFAPYG
Sbjct: 63  TYGPILHLRLGFVDVVVAASKSVAEQFLKIHDANFASRPPNSGAKHMAYNYQDLVFAPYG 122

Query: 130 QRWRLLRKISSLHLFSTKAMEDYKPIRQEEVGILTKALVKAESKEAVQLGQLLNVCTTNA 189
            RWRLLRKISS+HLFS KA+ED+K +RQEEVG LT+ LV+  +K  V LGQL+N+C  NA
Sbjct: 123 HRWRLLRKISSVHLFSAKALEDFKHVRQEEVGTLTRELVRVGTKP-VNLGQLVNMCVVNA 182

Query: 190 LSRVMLGKRVFGDGTGESDKKSEEFKDMVMELMVLAGEFNIGDFVPALEWLDLQGIATKM 249
           L R M+G+R+FG    ++D K++EF+ MV E+M LAG FNIGDFVP+L+WLDLQG+A KM
Sbjct: 183 LGREMIGRRLFG---ADADHKADEFRSMVTEMMALAGVFNIGDFVPSLDWLDLQGVAGKM 242

Query: 250 KKLHKRFDNFLGAILEEHKAKFKGGNFGGDFLSTLISLKD-NCDGEGGKLTDIEIKALLL 309
           K+LHKRFD FL +IL+EH+   +      D LSTLISLK  + DG+GG LTD EIKALLL
Sbjct: 243 KRLHKRFDAFLSSILKEHEMNGQDQKHT-DMLSTLISLKGTDLDGDGGSLTDTEIKALLL 302

Query: 310 NMFTAGTDTSSSTTEWAIAELIRHPKILAHVQQELDSIVGQDRLVNESDLPNLPYFQAVM 369
           NMFTAGTDTS+ST +WAIAELIRHP I+   Q+ELD +VG+DR VNESD+  LPY QAV+
Sbjct: 303 NMFTAGTDTSASTVDWAIAELIRHPDIMVKAQEELDIVVGRDRPVNESDIAQLPYLQAVI 362

Query: 370 KEVFRLHPSTPLSLPRVSAEPCEINGYYIPKNTTLLVNVWAIARDPNVWADPLEFRPERF 429
           KE FRLHP TPLSLP +++E CEINGY+IPK +TLL N+WAIARDP+ W+DPL F+PERF
Sbjct: 363 KENFRLHPPTPLSLPHIASESCEINGYHIPKGSTLLTNIWAIARDPDQWSDPLAFKPERF 422

Query: 430 LKGGERPNADVKGNDFEVIPFGAGRRICAGMSLGLRMVQLLTAALVHGFDWKLAHGLAQE 489
           L GGE+   DVKG+DFE+IPFGAGRRICAG+SLGLR +Q LTA LV GFDW+LA G+  E
Sbjct: 423 LPGGEKSGVDVKGSDFELIPFGAGRRICAGLSLGLRTIQFLTATLVQGFDWELAGGVTPE 482

Query: 490 KLNMEEAYGLTLQRKVPLMVYPLPRLPAKAY 516
           KLNMEE+YGLTLQR VPL+V+P PRL    Y
Sbjct: 483 KLNMEESYGLTLQRAVPLVVHPKPRLAPNVY 508

[Close]

BLAST of Spo05258.1 vs. TAIR (Arabidopsis)
Match: AT4G12320.1 (cytochrome P450, family 706, subfamily A, polypeptide 6)

HSP 1 Score: 364.0 bits (933), Expect = 1.500e-100
Identity = 201/512 (39.26%), Postives = 299/512 (58.40%), Query Frame = 1

		  
Query: 4   LEIISYTIAIAIAIAIITYYLITTIIFRHPRP-LPPGPKPWPIVGNLPHMGPVPHQGLAA 63
           + +  Y I I IAI  I +YL      R P+P LPPGP+  PIVGNLP + P  H     
Sbjct: 13  INLTPYAILILIAIFSILWYLFK----RSPQPHLPPGPRGLPIVGNLPFLDPDLHTYFTK 72

Query: 64  LAQKYGPLLYMRLGYVHVVVAASASVAAQFLKTHDANFSSRPPNSGAKHIAYNYQDMVFA 123
           LA+ YGP+  + LG    VV  + S+A + LK  D NFS+      A+ + Y   D+V+ 
Sbjct: 73  LAESYGPIFKLNLGSKLTVVVNTPSLAREILKDQDINFSNHDVPLTARAVTYGGLDLVWL 132

Query: 124 PYGQRWRLLRKISSLHLFSTKAMEDYKPIRQEEVGILTKALV-KAESKEAVQLGQLLNVC 183
           PYG  WR+LRK+  L L S + +  +  +R++E+   T+ L  K + +  V +G+ + + 
Sbjct: 133 PYGAEWRMLRKVCVLKLLSHRTLNSFYELRRKEIRERTRYLYQKGQEESPVNVGEQVFLT 192

Query: 184 TTNALSRVMLGKRVFGDGTGESDKKSEEFKDMVMELMVLAGEFNIGDFVPALEWLDLQGI 243
             N    ++ G  V  +   E +    EFK+++ E+  L GE N+ DF P L   DLQG+
Sbjct: 193 MMNLTMNMLWGGSVKAE---EMESVGTEFKEVISEITRLLGEPNVSDFFPRLARFDLQGL 252

Query: 244 ATKMKKLHKRFDNFLGAILEEHKA-KFKGGNFGG--DFLSTLISLKDNCDGEGGKLTDIE 303
             KM    +  D  L   +E+ +  + + G+ G   DFL  L+ LKD        +T   
Sbjct: 253 VKKMHVCARELDAILDRAIEQMQLLRTRDGDDGECKDFLQHLMKLKDQEADSEVPITVNH 312

Query: 304 IKALLLNMFTAGTDTSSSTTEWAIAELIRHPKILAHVQQELDSIVGQDRLVNESDLPNLP 363
           +KA+L+++   GTDTS++T E+A+AELIR P+++   QQELD +VG+D ++ ES +  LP
Sbjct: 313 VKAVLVDLVVGGTDTSTNTIEFAMAELIRKPELMKRAQQELDEVVGKDNIIEESHITRLP 372

Query: 364 YFQAVMKEVFRLHPSTPLSLPRVSAEPCEINGYYIPKNTTLLVNVWAIARDPNVWADPLE 423
           +  A+MKE  RL+P+ PL +P   +E   + GY IPKNT + +NVW+I RDPNVW  P E
Sbjct: 373 FISAIMKETLRLYPTIPLLVPHRPSETALVGGYTIPKNTKIFINVWSIQRDPNVWEYPTE 432

Query: 424 FRPERFLKGGERPNADVKGNDFEVIPFGAGRRICAGMSLGLRMVQLLTAALVHGFDWKLA 483
           FRPERFL   ++ + D  G D+  +PFG+GRRICAG++L  RM+    A L+H FDWK+ 
Sbjct: 433 FRPERFL---DKKSCDFTGTDYSYLPFGSGRRICAGIALAERMILYTLATLLHSFDWKIP 492

Query: 484 HGLAQEKLNMEEAYGLTLQRKVPLMVYPLPRL 511
            G     L+++E +G+ L+ K PL+  P+PRL
Sbjct: 493 EG---HILDLKEKFGIVLKLKSPLVALPVPRL 511

[Close]

BLAST of Spo05258.1 vs. TAIR (Arabidopsis)
Match: AT1G01280.1 (cytochrome P450, family 703, subfamily A, polypeptide 2)

HSP 1 Score: 361.3 bits (926), Expect = 9.900e-100
Identity = 192/489 (39.26%), Postives = 288/489 (58.90%), Query Frame = 1

		  
Query: 36  LPPGPKPWPIVGNLPHMGPVPHQGLAALAQKYGPLLYMRLGYVHVVVAASASVAAQFLKT 95
           LPPGP   PI+GNL  +GP+PH+ LA+L  KYGPL+Y+RLG V  +         + L  
Sbjct: 32  LPPGPPRLPILGNLLQLGPLPHRDLASLCDKYGPLVYLRLGNVDAITTNDPDTIREILLR 91

Query: 96  HDANFSSRPPNSGAKHIAYNYQDMVFAPYGQRWRLLRKISSLHLFSTKAMEDYKPIRQEE 155
            D  FSSRP    A H+AY   D+  AP G  W+ +R+I   HL +TK +E +   R EE
Sbjct: 92  QDDVFSSRPKTLAAVHLAYGCGDVALAPMGPHWKRMRRICMEHLLTTKRLESFTTQRAEE 151

Query: 156 VGILTKALVK-AESKEAVQLGQLLNVCTTNALSRVMLGKRVFGDGTGESDKKSEEFKDMV 215
              L + + K +E+ + + L ++L   + N ++R++LGK+ FG G+  S K+++EF  + 
Sbjct: 152 ARYLIRDVFKRSETGKPINLKEVLGAFSMNNVTRMLLGKQFFGPGSLVSPKEAQEFLHIT 211

Query: 216 MELMVLAGEFNIGDFVPALEWLDLQGIATKMKKLHKRFDNFLGAILEEHK-AKFKGGNFG 275
            +L  L G   +GD++P   W+D  G   +M+ + KR D F   I++EH+ AK +  +  
Sbjct: 212 HKLFWLLGVIYLGDYLPFWRWVDPSGCEKEMRDVEKRVDEFHTKIIDEHRRAKLEDEDKN 271

Query: 276 GD--FLSTLISLKDNCDGEGGK--LTDIEIKALLLNMFTAGTDTSSSTTEWAIAELIRHP 335
           GD  F+  L+SL     GE GK  + D+EIKAL+ +M  A TDTS+ T EWA+AE I+ P
Sbjct: 272 GDMDFVDVLLSLP----GENGKAHMEDVEIKALIQDMIAAATDTSAVTNEWAMAEAIKQP 331

Query: 336 KILAHVQQELDSIVGQDRLVNESDLPNLPYFQAVMKEVFRLHPSTPLSLPRVSAEPCEIN 395
           +++  +Q+ELD++VG +R+V+ESDL +L Y + V++E FR+HP+ P  +P  S     IN
Sbjct: 332 RVMRKIQEELDNVVGSNRMVDESDLVHLNYLRCVVRETFRMHPAGPFLIPHESVRATTIN 391

Query: 396 GYYIPKNTTLLVNVWAIARDPNVWADPLEFRPERF--LKGGERPNADVKGNDFEVIPFGA 455
           GYYIP  T + +N   + R+  +W D  +FRPER   ++G  R      G DF+++PF A
Sbjct: 392 GYYIPAKTRVFINTHGLGRNTKIWDDVEDFRPERHWPVEGSGRVEIS-HGPDFKILPFSA 451

Query: 456 GRRICAGMSLGLRMVQLLTAALVHGFDWKLAHGLAQEKLNMEEAYGLTLQRKVPLMVYPL 515
           G+R C G  LG+ MV +  A L H F+W      +   ++  E YG+T+ +  PL     
Sbjct: 452 GKRKCPGAPLGVTMVLMALARLFHCFEWS-----SPGNIDTVEVYGMTMPKAKPLRAIAK 510

Query: 516 PRLPAKAYT 517
           PRL A  YT
Sbjct: 512 PRLAAHLYT 510

[Close]

BLAST of Spo05258.1 vs. TAIR (Arabidopsis)
Match: AT4G12310.1 (cytochrome P450, family 706, subfamily A, polypeptide 5)

HSP 1 Score: 360.5 bits (924), Expect = 1.700e-99
Identity = 201/514 (39.11%), Postives = 300/514 (58.37%), Query Frame = 1

		  
Query: 3   PLEIISYT-IAIAIAIAIITYYLITTIIFRHPRP-LPPGPKPWPIVGNLPHMGPVPHQGL 62
           P   IS T  A A+ I   T+ ++  I  R P+P LPPGP+  PIVGNLP + P  H   
Sbjct: 9   PDNAISLTPYAYAVLILTATFSILWYIFKRSPQPPLPPGPRGLPIVGNLPFLDPDLHTYF 68

Query: 63  AALAQKYGPLLYMRLGYVHVVVAASASVAAQFLKTHDANFSSRPPNSGAKHIAYNYQDMV 122
             LAQ +GP+  + LG    VV  S S+A++ LK  D NFS+      A+ + Y   D+V
Sbjct: 69  TKLAQSHGPIFKLNLGSKLTVVVNSPSLASEILKDQDINFSNHDVPLTARAVTYGGLDLV 128

Query: 123 FAPYGQRWRLLRKISSLHLFSTKAMEDYKPIRQEEVGILTKALV-KAESKEAVQLGQLLN 182
           + PYG  WR+LRK+ +  LFS K ++ +  +R++E+   T+ L  K   K  V +G+ L 
Sbjct: 129 WLPYGAEWRMLRKVCAAKLFSRKTLDSFYELRRKEIRERTRCLYQKGLEKSPVNVGEQLF 188

Query: 183 VCTTNALSRVMLGKRVFGDGTGESDKKSEEFKDMVMELMVLAGEFNIGDFVPALEWLDLQ 242
           +   N +  ++ G  V  +   + +    EFK ++ E+  L G  N+ DF P L   DLQ
Sbjct: 189 LTMMNLMMNMLWGGSVKAE---DMESVGTEFKGVISEITRLLGVPNVSDFFPMLARFDLQ 248

Query: 243 GIATKMKKLHKRFDNFLGAILEE-HKAKFKGGNFGG--DFLSTLISLKDNCDGEGGKLTD 302
           G+  KM    +  D  L   +E+  + + + G+ G   DFL  L+ L+D        +T 
Sbjct: 249 GLVKKMHLYARDLDAILDRAIEQMQRLRSRDGDDGECKDFLQHLMKLRDQEADSDVPITM 308

Query: 303 IEIKALLLNMFTAGTDTSSSTTEWAIAELIRHPKILAHVQQELDSIVGQDRLVNESDLPN 362
             +KA+L++M   GT++S++T E+ +AELI +P+++   QQELD +VG+D +V ES + +
Sbjct: 309 NHVKAVLMDMVVGGTESSTNTIEFVMAELISNPELMRRAQQELDEVVGKDNIVEESHITS 368

Query: 363 LPYFQAVMKEVFRLHPSTPLSLPRVSAEPCEINGYYIPKNTTLLVNVWAIARDPNVWADP 422
           LPY  AV+KE  RL+P+ PL +P   +E   + GY IPKNT + +NVW+I RDPNVW  P
Sbjct: 369 LPYILAVLKETLRLYPTIPLLVPHRPSETALVGGYTIPKNTKIFINVWSIQRDPNVWEYP 428

Query: 423 LEFRPERFLKGGERPNADVKGNDFEVIPFGAGRRICAGMSLGLRMVQLLTAALVHGFDWK 482
            EFRPERFL   ++ + D  G D+  +PFG+GRRICAG++L  RM+    A L+H FDW 
Sbjct: 429 TEFRPERFL---DKKSCDFTGTDYSYLPFGSGRRICAGIALAERMILYTLATLLHSFDWT 488

Query: 483 LAHGLAQEKLNMEEAYGLTLQRKVPLMVYPLPRL 511
           +  G     L++EE +G+ L+ K PL+  P+PRL
Sbjct: 489 IPDG---HVLDLEEKFGIVLKLKTPLVALPIPRL 513

[Close]

BLAST of Spo05258.1 vs. TAIR (Arabidopsis)
Match: AT2G45550.1 (cytochrome P450, family 76, subfamily C, polypeptide 4)

HSP 1 Score: 358.6 bits (919), Expect = 6.400e-99
Identity = 194/482 (40.25%), Postives = 290/482 (60.17%), Query Frame = 1

		  
Query: 36  LPPGPKPWPIVGNLPHMGPVPHQGLAALAQKYGPLLYMRLGYVHVVVAASASVAAQFLKT 95
           LPPGP   PI+GN+  +G  PH   A LA+ YGP++ ++ G ++ VV  S   A + L+T
Sbjct: 38  LPPGPPRLPIIGNIHQVGKNPHSSFADLAKIYGPIMSLKFGCLNSVVITSPEAAREVLRT 97

Query: 96  HDANFSSRPPNSGAKHIAYNYQDMVFAPYGQ-RWRLLRKISSLHLFSTKAMEDYKPIRQE 155
           HD   S R  N   +   +    +++ P    RWR+LRK+S   +FS +  E  K +R +
Sbjct: 98  HDQILSGRKSNDSIRCFGHEEVSVIWLPPSSARWRMLRKLSVTLMFSPQRTEATKALRMK 157

Query: 156 EVGILTKALVKA-ESKEAVQLGQLLNVCTTNALSRVMLGKRVFGDGTGESD-KKSEEFKD 215
           +V  L   + ++ E KEAV + +       N +S ++     F    G  D KKS EF+D
Sbjct: 158 KVQELVSFMNESSERKEAVDISRASYTTVLNIISNIL-----FSVDLGSYDSKKSNEFQD 217

Query: 216 MVMELMVLAGEFNIGDFVPALEWLDLQGIATKMKKLHKRFDNFLGAILEEHKAKFKGGNF 275
            V+  M  AG+ +  ++ P + +LDLQG    M+ L +R        ++   A+   GN+
Sbjct: 218 TVIGAMEAAGKPDAANYFPFMGFLDLQGNRKAMRGLTERLFRVFRGFMDAKIAEKSLGNY 277

Query: 276 GGD-----FLSTLISLKDNCDGEGGKLTDIEIKALLLNMFTAGTDTSSSTTEWAIAELIR 335
             D     FL +L+ L +   G+  +L + +I+ LLL+MFTAGTDTSSST EWA+AEL+R
Sbjct: 278 SKDVSNRDFLDSLLILNE---GDEAELDNNDIEHLLLDMFTAGTDTSSSTLEWAMAELLR 337

Query: 336 HPKILAHVQQELDSIVGQDRLVNESDLPNLPYFQAVMKEVFRLHPSTPLSLPRVSAEPCE 395
           +PK +   Q E+D ++GQ+ +V ESD+  LPY QAV+KE FRLHP+ PL +PR +    E
Sbjct: 338 NPKTMVKAQAEMDRVLGQNSVVQESDISGLPYLQAVVKETFRLHPAAPLLVPRKAESDVE 397

Query: 396 INGYYIPKNTTLLVNVWAIARDPNVWADPLEFRPERFLKGGERPNADVKGNDFEVIPFGA 455
           + G+ +PK+T +LVNVWAI RDP+VW +P +F PERF+      + DVKG D+E+ PFG 
Sbjct: 398 VLGFMVPKDTQVLVNVWAIGRDPSVWENPSQFEPERFM----GKDIDVKGRDYELTPFGG 457

Query: 456 GRRICAGMSLGLRMVQLLTAALVHGFDWKLAHGLAQEKLNMEEAYGLTLQRKVPLMVYPL 510
           GRRIC G+ L ++ V L+ A+L++ FDWKL +G+  E L+M+E +G+TL R   L   P+
Sbjct: 458 GRRICPGLPLAVKTVSLMLASLLYSFDWKLPNGVVSEDLDMDETFGITLHRTNTLYAIPV 507

The following BLAST results are available for this feature:

BLAST of Spo05258.1 vs. NCBI nr
BLAST of Spo05258.1 vs. UniProtKB/TrEMBL
BLAST of Spo05258.1 vs. ExPASy Swiss-Prot
BLAST of Spo05258.1 vs. TAIR (Arabidopsis)

BLAST of Spo05258.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5

Position :

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x 1


Match Name	E-value	Identity	Description
gi\|731366673\|ref\|XP_010695197.1\|	3.5e-256	86.6	PREDICTED: flavonoid 3'-monoox...	[more]
gi\|1005743021\|gb\|AMQ23620.1\|	9.0e-236	79.1	flavonoid 3'hydroxylase [Silen...	[more]
gi\|523496545\|dbj\|BAN62871.1\|	1.1e-233	78.0	flavonoid 3'-hydroxylase [Dian...	[more]
gi\|470902337\|dbj\|BAN05297.1\|	2.1e-232	78.0	flavonoid 3'-hydroxylase [Dian...	[more]
gi\|590668630\|ref\|XP_007037548.1\|	1.4e-220	75.5	Flavonoid 3'-hydroxylase isofo...	[more]

BLAST of Spo05258.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5

Position :

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Match Name	E-value	Identity	Description
A0A142D8F1_9CARY	6.3e-236	79.1	Flavonoid 3'hydroxylase OS=Sil...	[more]
S6B1I9_DIACA	7.7e-234	78.0	Flavonoid 3'-hydroxylase OS=Di...	[more]
M5AAC5_DIACA	1.4e-232	78.0	Flavonoid 3'-hydroxylase OS=Di...	[more]
A0A061G4T4_THECC	9.7e-221	75.5	Flavonoid 3'-hydroxylase isofo...	[more]
A0A0H3WMD5_CAMSI	5.3e-219	72.9	Flavonoid 3'-hydroxylase 2 OS=...	[more]

BLAST of Spo05258.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5

Position :

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Match Name	E-value	Identity	Description
F3PH_PETHY	6.1e-208	69.4	Flavonoid 3'-monooxygenase OS=...	[more]
F3PH_ARATH	8.5e-194	65.1	Flavonoid 3'-monooxygenase OS=...	[more]
C75A1_PETHY	1.3e-146	51.4	Flavonoid 3',5'-hydroxylase 1 ...	[more]
C75A2_SOLME	2.3e-146	51.4	Flavonoid 3',5'-hydroxylase OS...	[more]
C75A3_PETHY	1.1e-145	51.4	Flavonoid 3',5'-hydroxylase 2 ...	[more]

BLAST of Spo05258.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 5

Position :

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Match Name	E-value	Identity	Description
AT5G07990.1	4.8e-195	65.1	Cytochrome P450 superfamily pr...	[more]
AT4G12320.1	1.5e-100	39.2	cytochrome P450, family 706, s...	[more]
AT1G01280.1	9.9e-100	39.2	cytochrome P450, family 703, s...	[more]
AT4G12310.1	1.7e-99	39.1	cytochrome P450, family 706, s...	[more]
AT2G45550.1	6.4e-99	40.2	cytochrome P450, family 76, su...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 443..452 score: 1.6E-11coord: 309..326 score: 1.6E-11coord: 452..463 score: 1.6E-11coord: 362..373 score: 1.6
IPR001128	Cytochrome P450	GENE3D	1.10.630.10		coord: 28..509 score: 1.3E
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 37..497 score: 6.6E
IPR001128	Cytochrome P450	unknown	SSF48264	Cytochrome P450	coord: 37..509 score: 8.91E
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 442..452 score: 1.1E-48coord: 298..315 score: 1.1E-48coord: 88..109 score: 1.1E-48coord: 402..426 score: 1.1E-48coord: 361..379 score: 1.1E-48coord: 181..199 score: 1.1E-48coord: 452..475 score: 1.1E-48coord: 318..344 score: 1.1E-48coord: 64..83 score: 1.1
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 445..454 scor
None	No IPR available	PANTHER	PTHR24298	FAMILY NOT NAMED	coord: 7..513 score:
None	No IPR available	PANTHER	PTHR24298:SF104	FLAVONOID 3'-MONOOXYGENASE	coord: 7..513 score:

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0009813	flavonoid biosynthetic process
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
molecular_function	GO:0016711	flavonoid 3'-monooxygenase activity