Spo05445.1 (mRNA)

Overview
NameSpo05445.1
TypemRNA
OrganismSpinacia oleracea (Spinach)
DescriptionHistone H2A
LocationSuper_scaffold_126 : 1476486 .. 1477685 (+)
Sequence length354
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTTGTGTTTTCTGTTCAAGATGATGAACTGATGATGAAATTGCATAATGCAGAATGAGATCGCGTTATTAAGTAAAATTCGGTAGCAGAATATTATAACTCTTCAAGGCTGTTGCATTCATGGTGATTCTCGATTTCTTGTGTATGAACTTATGGAAAATGGATGCCTGGAAACTCAGTTGCACGGTAATATTTCAAACATTCTTATATGTTAAGTTACTCGGACTTGGGTACTCATGTTAGACTAGGAAATGTATCTAGGATCAGACTCAGCTGCTCTATGTGAAAATTCCCATGTTAATAATCCAAAATTAAGTGTTAACCATCTGTAACTAATGTTTGAGTGTCAAGGATCCGATAAGGTGAAATGTAGAGTCCAGGTTGATCAGTGTTGAAAGTATAGTGTCAAGTTGAAAGTTCTATAGCATGAGAGGATGGAGAACCTACTATTTGTATTTGATCAATGTTTTTAGGTGTTTTCCTAGAATCAACTTTAACTTGCCATATACGAATGAAAATCTCTTTTGATATTGCAAGGTGACATTTTACAGCGCAAATATTTGGCTATTATTGATTAATTTGTCGATATCATAATTGAATTTGTTGTGGATCGAGTGTTGATTTGAGGAAATTTGTATAATACGCATACATGATTGTATGCTTTTAGTTGATAATTTTTATTCACTTTATGTGTGAAGGACTTTAAGGGAAATTAAAGGTATTTAAGAATTTAATTTGTTTGCTTTTAATGCAAGGATATTTAGCTTGAATTTACGTTGACTAATTAAAAGTTAAATGATTGAATTTGAAGCTGATTAGGTATTTGTGATTATGAATTAACATGTTCTGGAAGTGGATGAAAATGGCGCACGAGACAACAAGAAGATGAGAATCATACCAAGGCACATTCAATTGGCTATTAGGAACGATGAAGAGAATCATACCAGTTAAGCGAGGAGGCGGGTTTTGAGTATTATTGGTTGTTCTAAAAATATCATCCTGTCTTGATTTTATGTCTTCTTTTCCTGTCGTACTTTAAGTAGATTCTGCCTCTTTTATGTTGCAGGTAACAAAACAACACAGTAATGGCTGCAAAAAACTACTAGGATTGAACGATCCTGAAGAGAAGATGAGATTACTTCAGTTAGGGCAAGAGGAGGTTGCGAGAATGGAGAGGGAGCGGCTAAAGTTTAAGTAG

mRNA sequence

ATGCTTCAGAATATTATAACTCTTCAAGGCTGTTGCATTCATGGTGATTCTCGATTTCTTGTGTATGAACTTATGGAAAATGGATGCCTGGAAACTCAGTTGCACGTGGATGAAAATGGCGCACGAGACAACAAGAAGATGAGAATCATACCAAGGCACATTCAATTGGCTATTAGGAACGATGAAGAGAATCATACCAATTCTGCCTCTTTTATGTTGCAGGTAACAAAACAACACAGTAATGGCTGCAAAAAACTACTAGGATTGAACGATCCTGAAGAGAAGATGAGATTACTTCAGTTAGGGCAAGAGGAGGTTGCGAGAATGGAGAGGGAGCGGCTAAAGTTTAAGTAG

Coding sequence (CDS)

ATGCTTCAGAATATTATAACTCTTCAAGGCTGTTGCATTCATGGTGATTCTCGATTTCTTGTGTATGAACTTATGGAAAATGGATGCCTGGAAACTCAGTTGCACGTGGATGAAAATGGCGCACGAGACAACAAGAAGATGAGAATCATACCAAGGCACATTCAATTGGCTATTAGGAACGATGAAGAGAATCATACCAATTCTGCCTCTTTTATGTTGCAGGTAACAAAACAACACAGTAATGGCTGCAAAAAACTACTAGGATTGAACGATCCTGAAGAGAAGATGAGATTACTTCAGTTAGGGCAAGAGGAGGTTGCGAGAATGGAGAGGGAGCGGCTAAAGTTTAAGTAG

Protein sequence

MLQNIITLQGCCIHGDSRFLVYELMENGCLETQLHVDENGARDNKKMRIIPRHIQLAIRNDEENHTNSASFMLQVTKQHSNGCKKLLGLNDPEEKMRLLQLGQEEVARMERERLKFK
Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Spo05445Spo05445gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Spo05445.1Spo05445.1-proteinpolypeptide


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Spo05445.1.exon.1Spo05445.1.exon.1exon
Spo05445.1.exon.2Spo05445.1.exon.2exon
Spo05445.1.exon.3Spo05445.1.exon.3exon
Spo05445.1.exon.4Spo05445.1.exon.4exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Spo05445.1.CDS.1Spo05445.1.CDS.1CDS
Spo05445.1.CDS.2Spo05445.1.CDS.2CDS
Spo05445.1.CDS.3Spo05445.1.CDS.3CDS
Spo05445.1.CDS.4Spo05445.1.CDS.4CDS


Homology
[Close]
BLAST of Spo05445.1 vs. NCBI nr
Match: gi|731316793|ref|XP_010665786.1| (PREDICTED: probable receptor-like protein kinase At1g80640 isoform X1 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 70.5 bits (171), Expect = 2.200e-9
Identity = 30/39 (76.92%), Postives = 33/39 (84.62%), Query Frame = 1 

		  

Query: 3   QNIITLQGCCIHGDSRFLVYELMENGCLETQLHVDENGA 42
           QNII L GCCIHGD+RFLVYE+MENGCLETQLH    G+
Sbjct: 202 QNIINLLGCCIHGDTRFLVYEIMENGCLETQLHGVSRGS 240
[Close]
BLAST of Spo05445.1 vs. NCBI nr
Match: gi|731316795|ref|XP_010665794.1| (PREDICTED: probable receptor-like protein kinase At1g80640 isoform X2 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 70.5 bits (171), Expect = 2.200e-9
Identity = 30/39 (76.92%), Postives = 33/39 (84.62%), Query Frame = 1 

		  

Query: 3   QNIITLQGCCIHGDSRFLVYELMENGCLETQLHVDENGA 42
           QNII L GCCIHGD+RFLVYE+MENGCLETQLH    G+
Sbjct: 202 QNIINLLGCCIHGDTRFLVYEIMENGCLETQLHGVSRGS 240
[Close]
BLAST of Spo05445.1 vs. NCBI nr
Match: gi|902187753|gb|KNA10981.1| (hypothetical protein SOVF_139360 [Spinacia oleracea])

HSP 1 Score: 70.1 bits (170), Expect = 2.900e-9
Identity = 32/39 (82.05%), Postives = 33/39 (84.62%), Query Frame = 1 

		  

Query: 3   QNIITLQGCCIHGDSRFLVYELMENGCLETQLHVDENGA 42
           QNIITL G CIHGDSRFLVYELMENGCLETQLH    G+
Sbjct: 161 QNIITLLGYCIHGDSRFLVYELMENGCLETQLHGVSRGS 199
[Close]
BLAST of Spo05445.1 vs. NCBI nr
Match: gi|1021572801|ref|XP_016177494.1| (PREDICTED: probable receptor-like protein kinase At1g80640 [Arachis ipaensis])

HSP 1 Score: 63.9 bits (154), Expect = 2.100e-7
Identity = 29/39 (74.36%), Postives = 31/39 (79.49%), Query Frame = 1 

		  

Query: 3   QNIITLQGCCIHGDSRFLVYELMENGCLETQLHVDENGA 42
           QNII L G CIHG+SRFLVYELMENG LETQLH    G+
Sbjct: 171 QNIIKLMGYCIHGESRFLVYELMENGSLETQLHGPNRGS 209
[Close]
BLAST of Spo05445.1 vs. NCBI nr
Match: gi|950971361|ref|XP_014500297.1| (PREDICTED: probable receptor-like protein kinase At1g80640 [Vigna radiata var. radiata])

HSP 1 Score: 63.9 bits (154), Expect = 2.100e-7
Identity = 29/39 (74.36%), Postives = 30/39 (76.92%), Query Frame = 1 

		  

Query: 3   QNIITLQGCCIHGDSRFLVYELMENGCLETQLHVDENGA 42
           QNII L G C+HG SRFLVYELMENG LETQLH    GA
Sbjct: 189 QNIIKLMGYCVHGQSRFLVYELMENGSLETQLHGPNRGA 227
[Close]
BLAST of Spo05445.1 vs. UniProtKB/TrEMBL
Match: A0A0J8FTJ6_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_2g031070 PE=3 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 1.600e-9
Identity = 30/39 (76.92%), Postives = 33/39 (84.62%), Query Frame = 1 

		  

Query: 3   QNIITLQGCCIHGDSRFLVYELMENGCLETQLHVDENGA 42
           QNII L GCCIHGD+RFLVYE+MENGCLETQLH    G+
Sbjct: 202 QNIINLLGCCIHGDTRFLVYEIMENGCLETQLHGVSRGS 240
[Close]
BLAST of Spo05445.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QWS0_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_139360 PE=3 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 2.000e-9
Identity = 32/39 (82.05%), Postives = 33/39 (84.62%), Query Frame = 1 

		  

Query: 3   QNIITLQGCCIHGDSRFLVYELMENGCLETQLHVDENGA 42
           QNIITL G CIHGDSRFLVYELMENGCLETQLH    G+
Sbjct: 161 QNIITLLGYCIHGDSRFLVYELMENGCLETQLHGVSRGS 199
[Close]
BLAST of Spo05445.1 vs. UniProtKB/TrEMBL
Match: I1M5X9_SOYBN (Uncharacterized protein OS=Glycine max GN=GLYMA_13G369100 PE=4 SV=2)

HSP 1 Score: 63.2 bits (152), Expect = 2.500e-7
Identity = 28/39 (71.79%), Postives = 31/39 (79.49%), Query Frame = 1 

		  

Query: 3   QNIITLQGCCIHGDSRFLVYELMENGCLETQLHVDENGA 42
           QNII + G CIHG+SRFLVYELMENG LETQLH    G+
Sbjct: 189 QNIIKIMGYCIHGESRFLVYELMENGSLETQLHGPNRGS 227
[Close]
BLAST of Spo05445.1 vs. UniProtKB/TrEMBL
Match: F6HXT5_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_09s0002g09090 PE=3 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 2.500e-7
Identity = 25/39 (64.10%), Postives = 33/39 (84.62%), Query Frame = 1 

		  

Query: 3   QNIITLQGCCIHGDSRFLVYELMENGCLETQLHVDENGA 42
           QNI++L GCCIHG++RFLVYE+M+NG LE QLH   +G+
Sbjct: 152 QNIVSLLGCCIHGETRFLVYEMMQNGSLEAQLHGPSHGS 190
[Close]
BLAST of Spo05445.1 vs. UniProtKB/TrEMBL
Match: A0A0B2RF00_GLYSO (Putative receptor-like protein kinase OS=Glycine soja GN=glysoja_036208 PE=4 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 2.500e-7
Identity = 28/39 (71.79%), Postives = 31/39 (79.49%), Query Frame = 1 

		  

Query: 3   QNIITLQGCCIHGDSRFLVYELMENGCLETQLHVDENGA 42
           QNII + G CIHG+SRFLVYELMENG LETQLH    G+
Sbjct: 189 QNIIKMMGYCIHGESRFLVYELMENGSLETQLHGPNRGS 227
[Close]
BLAST of Spo05445.1 vs. TAIR (Arabidopsis)
Match: AT5G54640.1 (Histone superfamily protein)

HSP 1 Score: 49.3 bits (116), Expect = 1.900e-6
Identity = 22/30 (73.33%), Postives = 25/30 (83.33%), Query Frame = 1 

		  

Query: 34 LHVDENGARDNKKMRIIPRHIQLAIRNDEE 64
          L +  N ARDNKK RI+PRHIQLA+RNDEE
Sbjct: 65 LELAGNAARDNKKTRIVPRHIQLAVRNDEE 94
[Close]
BLAST of Spo05445.1 vs. TAIR (Arabidopsis)
Match: AT1G51060.1 (histone H2A 10)

HSP 1 Score: 49.3 bits (116), Expect = 1.900e-6
Identity = 22/30 (73.33%), Postives = 25/30 (83.33%), Query Frame = 1 

		  

Query: 34 LHVDENGARDNKKMRIIPRHIQLAIRNDEE 64
          L +  N ARDNKK RI+PRHIQLA+RNDEE
Sbjct: 65 LELAGNAARDNKKTRIVPRHIQLAVRNDEE 94
[Close]
BLAST of Spo05445.1 vs. TAIR (Arabidopsis)
Match: AT1G08880.1 (Histone superfamily protein)

HSP 1 Score: 49.3 bits (116), Expect = 1.900e-6
Identity = 22/30 (73.33%), Postives = 25/30 (83.33%), Query Frame = 1 

		  

Query: 34  LHVDENGARDNKKMRIIPRHIQLAIRNDEE 64
           L +  N ARDNKK RI+PRHIQLA+RNDEE
Sbjct: 71  LELAGNAARDNKKTRIVPRHIQLAVRNDEE 100
[Close]
BLAST of Spo05445.1 vs. TAIR (Arabidopsis)
Match: AT1G54690.1 (gamma histone variant H2AX)

HSP 1 Score: 49.3 bits (116), Expect = 1.900e-6
Identity = 22/30 (73.33%), Postives = 25/30 (83.33%), Query Frame = 1 

		  

Query: 34  LHVDENGARDNKKMRIIPRHIQLAIRNDEE 64
           L +  N ARDNKK RI+PRHIQLA+RNDEE
Sbjct: 71  LELAGNAARDNKKTRIVPRHIQLAVRNDEE 100
[Close]
BLAST of Spo05445.1 vs. TAIR (Arabidopsis)
Match: AT4G27230.1 (histone H2A 2)

HSP 1 Score: 49.3 bits (116), Expect = 1.900e-6
Identity = 22/30 (73.33%), Postives = 25/30 (83.33%), Query Frame = 1 

		  

Query: 34 LHVDENGARDNKKMRIIPRHIQLAIRNDEE 64
          L +  N ARDNKK RI+PRHIQLA+RNDEE
Sbjct: 65 LELAGNAARDNKKTRIVPRHIQLAVRNDEE 94
The following BLAST results are available for this feature:
BLAST of Spo05445.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5
Match NameE-valueIdentityDescription
gi|731316793|ref|XP_010665786.1|2.2e-976.9PREDICTED: probable receptor-l... [more]
gi|731316795|ref|XP_010665794.1|2.2e-976.9PREDICTED: probable receptor-l... [more]
gi|902187753|gb|KNA10981.1|2.9e-982.0hypothetical protein SOVF_1393... [more]
gi|1021572801|ref|XP_016177494.1|2.1e-774.3PREDICTED: probable receptor-l... [more]
gi|950971361|ref|XP_014500297.1|2.1e-774.3PREDICTED: probable receptor-l... [more]
back to top
BLAST of Spo05445.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0J8FTJ6_BETVU1.6e-976.9Uncharacterized protein OS=Bet... [more]
A0A0K9QWS0_SPIOL2.0e-982.0Uncharacterized protein OS=Spi... [more]
I1M5X9_SOYBN2.5e-771.7Uncharacterized protein OS=Gly... [more]
F6HXT5_VITVI2.5e-764.1Putative uncharacterized prote... [more]
A0A0B2RF00_GLYSO2.5e-771.7Putative receptor-like protein... [more]
back to top
BLAST of Spo05445.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Spo05445.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 5
Match NameE-valueIdentityDescription
AT5G54640.11.9e-673.3Histone superfamily protein[more]
AT1G51060.11.9e-673.3histone H2A 10[more]
AT1G08880.11.9e-673.3Histone superfamily protein[more]
AT1G54690.11.9e-673.3gamma histone variant H2AX[more]
AT4G27230.11.9e-673.3histone H2A 2[more]
back to top
InterPro
Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001245Serine-threonine/tyrosine-protein kinase catalytic domainPFAMPF07714Pkinase_Tyrcoord: 3..84
score: 1.
IPR009072Histone-foldGENE3D1.10.20.10coord: 38..66
score: 9.7
IPR009072Histone-foldunknownSSF47113Histone-foldcoord: 39..63
score: 1.3
IPR011009Protein kinase-like domainunknownSSF56112Protein kinase-like (PK-like)coord: 3..41
score: 8.5
NoneNo IPR availableGENE3D3.30.200.20coord: 3..27
score: 2.
NoneNo IPR availablePANTHERPTHR23430HISTONE H2Acoord: 41..63
score: 1.5

GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
biological_process GO:0009069 serine family amino acid metabolic process
cellular_component GO:0016020 membrane
molecular_function GO:0046982 protein heterodimerization activity
molecular_function GO:0004672 protein kinase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity