Spo05949.1 (mRNA) | SpinachBase

Overview


Name	Spo05949.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	Mitochondrial import receptor subunit TOM9-2 (Mitochondrial import receptor subunit TOM22 homolog 2) (Translocase of outer membrane 22 kDa subunit homolog 2) (Translocase of outer membrane 9 kDa subunit TOM9-2)
Location	chr1 : 49411354 .. 49411656 (+)
Sequence length	303

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGTCAGGAAGAAAGAGAATAGGATCAAGCAGCGACAATGGAGGCGGAGTATTAGCAAGAGTGACAAACACAGTTTCAGATTCACCAATCTTCCAAAAAGGGGTACAATCTGCTAGCGATGTGGCTGGTGTCACCAAGAAGCTGCTCAAGAGCACCGGAAAAGCTGCTTGGATCGTCGGCACCACTTTCCTCGTCCTTGCTCTCCCTCTCATCATCGAGCTCGAGCGCGATGGTCAGTTGGATGCTATAGAGCTTCAGCAGGCTAGTCTTCTCGGCACCCCTCCCCCCGCCGCCCATTAA

mRNA sequence

ATGGCGTCAGGAAGAAAGAGAATAGGATCAAGCAGCGACAATGGAGGCGGAGTATTAGCAAGAGTGACAAACACAGTTTCAGATTCACCAATCTTCCAAAAAGGGGTACAATCTGCTAGCGATGTGGCTGGTGTCACCAAGAAGCTGCTCAAGAGCACCGGAAAAGCTGCTTGGATCGTCGGCACCACTTTCCTCGTCCTTGCTCTCCCTCTCATCATCGAGCTCGAGCGCGATGGTCAGTTGGATGCTATAGAGCTTCAGCAGGCTAGTCTTCTCGGCACCCCTCCCCCCGCCGCCCATTAA

Coding sequence (CDS)

ATGGCGTCAGGAAGAAAGAGAATAGGATCAAGCAGCGACAATGGAGGCGGAGTATTAGCAAGAGTGACAAACACAGTTTCAGATTCACCAATCTTCCAAAAAGGGGTACAATCTGCTAGCGATGTGGCTGGTGTCACCAAGAAGCTGCTCAAGAGCACCGGAAAAGCTGCTTGGATCGTCGGCACCACTTTCCTCGTCCTTGCTCTCCCTCTCATCATCGAGCTCGAGCGCGATGGTCAGTTGGATGCTATAGAGCTTCAGCAGGCTAGTCTTCTCGGCACCCCTCCCCCCGCCGCCCATTAA

Protein sequence

MASGRKRIGSSSDNGGGVLARVTNTVSDSPIFQKGVQSASDVAGVTKKLLKSTGKAAWIVGTTFLVLALPLIIELERDGQLDAIELQQASLLGTPPPAAH

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo05949	Spo05949	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo05949.1	Spo05949.1-protein	polypeptide

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo05949.1.exon.1	Spo05949.1.exon.1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo05949.1.CDS.1	Spo05949.1.CDS.1	CDS

Homology

[Close]

BLAST of Spo05949.1 vs. NCBI nr
Match: gi|902183133|gb|KNA10063.1| (hypothetical protein SOVF_147920 [Spinacia oleracea])

HSP 1 Score: 189.5 bits (480), Expect = 2.800e-45
Identity = 100/100 (100.00%), Postives = 100/100 (100.00%), Query Frame = 1


		  
Query: 1   MASGRKRIGSSSDNGGGVLARVTNTVSDSPIFQKGVQSASDVAGVTKKLLKSTGKAAWIV 60
           MASGRKRIGSSSDNGGGVLARVTNTVSDSPIFQKGVQSASDVAGVTKKLLKSTGKAAWIV
Sbjct: 1   MASGRKRIGSSSDNGGGVLARVTNTVSDSPIFQKGVQSASDVAGVTKKLLKSTGKAAWIV 60

Query: 61  GTTFLVLALPLIIELERDGQLDAIELQQASLLGTPPPAAH 101
           GTTFLVLALPLIIELERDGQLDAIELQQASLLGTPPPAAH
Sbjct: 61  GTTFLVLALPLIIELERDGQLDAIELQQASLLGTPPPAAH 100

[Close]

BLAST of Spo05949.1 vs. NCBI nr
Match: gi|902184558|gb|KNA10312.1| (hypothetical protein SOVF_145450 [Spinacia oleracea])

HSP 1 Score: 183.7 bits (465), Expect = 1.600e-43
Identity = 97/100 (97.00%), Postives = 98/100 (98.00%), Query Frame = 1


		  
Query: 1   MASGRKRIGSSSDNGGGVLARVTNTVSDSPIFQKGVQSASDVAGVTKKLLKSTGKAAWIV 60
           MAS RKRIGSSSDNGGGVLARVT+TVSDSPIFQKGVQSASDVAGVTKKLLKSTGKAAWIV
Sbjct: 1   MASNRKRIGSSSDNGGGVLARVTSTVSDSPIFQKGVQSASDVAGVTKKLLKSTGKAAWIV 60

Query: 61  GTTFLVLALPLIIELERDGQLDAIELQQASLLGTPPPAAH 101
           GTTFLVLALPLIIELERDGQLDAIELQQASLLGTPPPA H
Sbjct: 61  GTTFLVLALPLIIELERDGQLDAIELQQASLLGTPPPAGH 100

[Close]

BLAST of Spo05949.1 vs. NCBI nr
Match: gi|731372562|ref|XP_010666386.1| (PREDICTED: mitochondrial import receptor subunit TOM9-2-like [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 166.4 bits (420), Expect = 2.600e-38
Identity = 89/99 (89.90%), Postives = 95/99 (95.96%), Query Frame = 1


		  
Query: 2   ASGRKRIGSSSDNGGG-VLARVTNTVSDSPIFQKGVQSASDVAGVTKKLLKSTGKAAWIV 61
           +SGRKRIGSSSDNGGG V+ARVT+TVSDSPIFQKGVQSAS  A +TKKLLKSTGKAAWIV
Sbjct: 4   SSGRKRIGSSSDNGGGGVIARVTSTVSDSPIFQKGVQSASGAADITKKLLKSTGKAAWIV 63

Query: 62  GTTFLVLALPLIIELERDGQLDAIELQQASLLGTPPPAA 100
           GTTFLVLALPLIIELERDGQLDA+ELQQASLLGTPPPA+
Sbjct: 64  GTTFLVLALPLIIELERDGQLDALELQQASLLGTPPPAS 102

[Close]

BLAST of Spo05949.1 vs. NCBI nr
Match: gi|720020578|ref|XP_010262447.1| (PREDICTED: mitochondrial import receptor subunit TOM9-2-like [Nelumbo nucifera])

HSP 1 Score: 115.5 bits (288), Expect = 5.200e-23
Identity = 62/98 (63.27%), Postives = 76/98 (77.55%), Query Frame = 1


		  
Query: 1  MASGRKRIGSSSDNGGGVLARVTNTVSDSPIFQKGVQSASDVAGVTKKLLKSTGKAAWIV 60
          MAS  ++ G +     GVLARVTNT+S S I Q+G   ASD A VTKKLLKSTGKAAWI 
Sbjct: 1  MASQSRKGGKNRSGQEGVLARVTNTISQSSIVQQGKLVASDAAFVTKKLLKSTGKAAWIA 60

Query: 61 GTTFLVLALPLIIELERDGQLDAIELQQASLLGTPPPA 99
          GTTFL+L +PLIIE++R+ Q++ +E+QQASLLGTPP A
Sbjct: 61 GTTFLILVVPLIIEMDREQQMNELEMQQASLLGTPPVA 98

[Close]

BLAST of Spo05949.1 vs. NCBI nr
Match: gi|698513962|ref|XP_009801898.1| (PREDICTED: mitochondrial import receptor subunit TOM9-2-like [Nicotiana sylvestris])

HSP 1 Score: 114.4 bits (285), Expect = 1.200e-22
Identity = 55/85 (64.71%), Postives = 72/85 (84.71%), Query Frame = 1


		  
Query: 15  GGGVLARVTNTVSDSPIFQKGVQSASDVAGVTKKLLKSTGKAAWIVGTTFLVLALPLIIE 74
           G G+++RV++++S SPI  +G ++ASD A + KKLLKSTGKAAWI GTTFL+L +PLIIE
Sbjct: 19  GDGIISRVSSSISQSPIVYQGKRAASDAALIAKKLLKSTGKAAWIAGTTFLILVVPLIIE 78

Query: 75  LERDGQLDAIELQQASLLGTPPPAA 100
           ++RD QL  +ELQQASLLGTPPPA+
Sbjct: 79  MDRDAQLTELELQQASLLGTPPPAS 103

[Close]

BLAST of Spo05949.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QTM4_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_147920 PE=4 SV=1)

HSP 1 Score: 189.5 bits (480), Expect = 2.000e-45
Identity = 100/100 (100.00%), Postives = 100/100 (100.00%), Query Frame = 1


		  
Query: 1   MASGRKRIGSSSDNGGGVLARVTNTVSDSPIFQKGVQSASDVAGVTKKLLKSTGKAAWIV 60
           MASGRKRIGSSSDNGGGVLARVTNTVSDSPIFQKGVQSASDVAGVTKKLLKSTGKAAWIV
Sbjct: 1   MASGRKRIGSSSDNGGGVLARVTNTVSDSPIFQKGVQSASDVAGVTKKLLKSTGKAAWIV 60

Query: 61  GTTFLVLALPLIIELERDGQLDAIELQQASLLGTPPPAAH 101
           GTTFLVLALPLIIELERDGQLDAIELQQASLLGTPPPAAH
Sbjct: 61  GTTFLVLALPLIIELERDGQLDAIELQQASLLGTPPPAAH 100

[Close]

BLAST of Spo05949.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QSN9_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_145450 PE=4 SV=1)

HSP 1 Score: 183.7 bits (465), Expect = 1.100e-43
Identity = 97/100 (97.00%), Postives = 98/100 (98.00%), Query Frame = 1


		  
Query: 1   MASGRKRIGSSSDNGGGVLARVTNTVSDSPIFQKGVQSASDVAGVTKKLLKSTGKAAWIV 60
           MAS RKRIGSSSDNGGGVLARVT+TVSDSPIFQKGVQSASDVAGVTKKLLKSTGKAAWIV
Sbjct: 1   MASNRKRIGSSSDNGGGVLARVTSTVSDSPIFQKGVQSASDVAGVTKKLLKSTGKAAWIV 60

Query: 61  GTTFLVLALPLIIELERDGQLDAIELQQASLLGTPPPAAH 101
           GTTFLVLALPLIIELERDGQLDAIELQQASLLGTPPPA H
Sbjct: 61  GTTFLVLALPLIIELERDGQLDAIELQQASLLGTPPPAGH 100

[Close]

BLAST of Spo05949.1 vs. UniProtKB/TrEMBL
Match: A0A0J8B4L3_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_001700 PE=4 SV=1)

HSP 1 Score: 166.4 bits (420), Expect = 1.800e-38
Identity = 89/99 (89.90%), Postives = 95/99 (95.96%), Query Frame = 1


		  
Query: 2   ASGRKRIGSSSDNGGG-VLARVTNTVSDSPIFQKGVQSASDVAGVTKKLLKSTGKAAWIV 61
           +SGRKRIGSSSDNGGG V+ARVT+TVSDSPIFQKGVQSAS  A +TKKLLKSTGKAAWIV
Sbjct: 4   SSGRKRIGSSSDNGGGGVIARVTSTVSDSPIFQKGVQSASGAADITKKLLKSTGKAAWIV 63

Query: 62  GTTFLVLALPLIIELERDGQLDAIELQQASLLGTPPPAA 100
           GTTFLVLALPLIIELERDGQLDA+ELQQASLLGTPPPA+
Sbjct: 64  GTTFLVLALPLIIELERDGQLDALELQQASLLGTPPPAS 102

[Close]

BLAST of Spo05949.1 vs. UniProtKB/TrEMBL
Match: K4C3J2_SOLLC (Uncharacterized protein OS=Solanum lycopersicum PE=4 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 1.800e-22
Identity = 56/92 (60.87%), Postives = 74/92 (80.43%), Query Frame = 1


		  
Query: 7   RIGSSSDNGGGVLARVTNTVSDSPIFQKGVQSASDVAGVTKKLLKSTGKAAWIVGTTFLV 66
           R G+ +  G G+L+R ++++S+SPI  +G ++ASDVA V KKL KSTGKAAWI GTTFL+
Sbjct: 13  RPGAGAGAGDGILSRFSSSISESPIVYQGKRAASDVAFVAKKLFKSTGKAAWIAGTTFLI 72

Query: 67  LALPLIIELERDGQLDAIELQQASLLGTPPPA 99
           L +PLIIE++RD QL  +E+QQASLLG PPPA
Sbjct: 73  LVVPLIIEMDRDAQLTELEMQQASLLGAPPPA 104

[Close]

BLAST of Spo05949.1 vs. UniProtKB/TrEMBL
Match: A0A103YIA1_CYNCS (Mitochondrial outer membrane translocase complex, subunit Tom22, plant OS=Cynara cardunculus var. scolymus GN=Ccrd_012002 PE=4 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 3.100e-22
Identity = 57/89 (64.04%), Postives = 74/89 (83.15%), Query Frame = 1


		  
Query: 10 SSSDNGGGVLARVTNTVSDSPIFQKGVQSASDVAGVTKKLLKSTGKAAWIVGTTFLVLAL 69
          SSSD   GVL+R+++T+S+SP+  +G Q+ASD   V KKLLKSTGKAAWI GTTFL+L +
Sbjct: 6  SSSD---GVLSRISSTISESPVVYRGKQAASDAGFVVKKLLKSTGKAAWIAGTTFLILVV 65

Query: 70 PLIIELERDGQLDAIELQQASLLGTPPPA 99
          PLIIE++R+ QL+ ++LQQASLLGTP PA
Sbjct: 66 PLIIEMDREAQLNELDLQQASLLGTPTPA 91

[Close]

BLAST of Spo05949.1 vs. ExPASy Swiss-Prot
Match: TOM92_ARATH (Mitochondrial import receptor subunit TOM9-2 OS=Arabidopsis thaliana GN=TOM9-2 PE=1 SV=3)

HSP 1 Score: 99.0 bits (245), Expect = 3.100e-20
Identity = 53/93 (56.99%), Postives = 69/93 (74.19%), Query Frame = 1


		  
Query: 6  KRIGSSSDNGG--GVLARVTNTVSDSPIFQKGVQSASDVAGVTKKLLKSTGKAAWIVGTT 65
          KRIG+    GG   +LAR++N    S I  +G ++A D   V+KKLL+STGKAAWI GTT
Sbjct: 4  KRIGAGKSGGGDPNILARISN----SEIVSQGRRAAGDAVEVSKKLLRSTGKAAWIAGTT 63

Query: 66 FLVLALPLIIELERDGQLDAIELQQASLLGTPP 97
          FL+L +PLIIE++R+ Q++ IELQQASLLG PP
Sbjct: 64 FLILVVPLIIEMDREAQINEIELQQASLLGAPP 92

[Close]

BLAST of Spo05949.1 vs. ExPASy Swiss-Prot
Match: TOM91_ARATH (Mitochondrial import receptor subunit TOM9-1 OS=Arabidopsis thaliana GN=TOM9-1 PE=2 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 1.900e-17
Identity = 50/94 (53.19%), Postives = 68/94 (72.34%), Query Frame = 1


		  
Query: 6   KRIGSSSDNGGGVLARVTNTVSDSPIFQKGVQSASDVAGVTKKLLKSTGKAAWIVGTTFL 65
           K+IG+   +   +LA+++N      I  +G ++A D   V+KKLLKSTGKAAWI GTTFL
Sbjct: 4   KKIGAGKGDSS-ILAKISNY----DIVSQGRRAACDAVYVSKKLLKSTGKAAWIAGTTFL 63

Query: 66  VLALPLIIELERDGQLDAIELQQASLLGTPPPAA 100
           +LA+PLI+ELE+D +L  I+ +QASLLGTPP  A
Sbjct: 64  ILAVPLILELEQDHRLGEIDFEQASLLGTPPVGA 92

[Close]

BLAST of Spo05949.1 vs. TAIR (Arabidopsis)
Match: AT5G43970.1 (translocase of outer membrane 22-V)

HSP 1 Score: 99.0 bits (245), Expect = 1.800e-21
Identity = 53/93 (56.99%), Postives = 69/93 (74.19%), Query Frame = 1


		  
Query: 6  KRIGSSSDNGG--GVLARVTNTVSDSPIFQKGVQSASDVAGVTKKLLKSTGKAAWIVGTT 65
          KRIG+    GG   +LAR++N    S I  +G ++A D   V+KKLL+STGKAAWI GTT
Sbjct: 4  KRIGAGKSGGGDPNILARISN----SEIVSQGRRAAGDAVEVSKKLLRSTGKAAWIAGTT 63

Query: 66 FLVLALPLIIELERDGQLDAIELQQASLLGTPP 97
          FL+L +PLIIE++R+ Q++ IELQQASLLG PP
Sbjct: 64 FLILVVPLIIEMDREAQINEIELQQASLLGAPP 92

[Close]

BLAST of Spo05949.1 vs. TAIR (Arabidopsis)
Match: AT1G04070.1 (translocase of outer membrane 22-I)

HSP 1 Score: 89.7 bits (221), Expect = 1.100e-18
Identity = 50/94 (53.19%), Postives = 68/94 (72.34%), Query Frame = 1


		  
Query: 6   KRIGSSSDNGGGVLARVTNTVSDSPIFQKGVQSASDVAGVTKKLLKSTGKAAWIVGTTFL 65
           K+IG+   +   +LA+++N      I  +G ++A D   V+KKLLKSTGKAAWI GTTFL
Sbjct: 4   KKIGAGKGDSS-ILAKISNY----DIVSQGRRAACDAVYVSKKLLKSTGKAAWIAGTTFL 63

Query: 66  VLALPLIIELERDGQLDAIELQQASLLGTPPPAA 100
           +LA+PLI+ELE+D +L  I+ +QASLLGTPP  A
Sbjct: 64  ILAVPLILELEQDHRLGEIDFEQASLLGTPPVGA 92

The following BLAST results are available for this feature:

BLAST of Spo05949.1 vs. NCBI nr
BLAST of Spo05949.1 vs. UniProtKB/TrEMBL
BLAST of Spo05949.1 vs. ExPASy Swiss-Prot
BLAST of Spo05949.1 vs. TAIR (Arabidopsis)

BLAST of Spo05949.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902183133\|gb\|KNA10063.1\|	2.8e-45	100.	hypothetical protein SOVF_1479...	[more]
gi\|902184558\|gb\|KNA10312.1\|	1.6e-43	97.0	hypothetical protein SOVF_1454...	[more]
gi\|731372562\|ref\|XP_010666386.1\|	2.6e-38	89.9	PREDICTED: mitochondrial impor...	[more]
gi\|720020578\|ref\|XP_010262447.1\|	5.2e-23	63.2	PREDICTED: mitochondrial impor...	[more]
gi\|698513962\|ref\|XP_009801898.1\|	1.2e-22	64.7	PREDICTED: mitochondrial impor...	[more]

BLAST of Spo05949.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9QTM4_SPIOL	2.0e-45	100.	Uncharacterized protein OS=Spi...	[more]
A0A0K9QSN9_SPIOL	1.1e-43	97.0	Uncharacterized protein OS=Spi...	[more]
A0A0J8B4L3_BETVU	1.8e-38	89.9	Uncharacterized protein OS=Bet...	[more]
K4C3J2_SOLLC	1.8e-22	60.8	Uncharacterized protein OS=Sol...	[more]
A0A103YIA1_CYNCS	3.1e-22	64.0	Mitochondrial outer membrane t...	[more]

BLAST of Spo05949.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 2


Match Name	E-value	Identity	Description
TOM92_ARATH	3.1e-20	56.9	Mitochondrial import receptor ...	[more]
TOM91_ARATH	1.9e-17	53.1	Mitochondrial import receptor ...	[more]

BLAST of Spo05949.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 2


Match Name	E-value	Identity	Description
AT5G43970.1	1.8e-21	56.9	translocase of outer membrane ...	[more]
AT1G04070.1	1.1e-18	53.1	translocase of outer membrane ...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005683	Mitochondrial import receptor subunit Tom22	PFAM	PF04281	Tom22	coord: 37..88 score: 5.
IPR017411	Mitochondrial outer membrane translocase complex, subunit Tom22, plant	PIR	PIRSF038151	TOM9-2_plant	coord: 1..100 score: 5.6
None	No IPR available	PANTHER	PTHR12504	MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM22	coord: 37..99 score: 3.1

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006886	intracellular protein transport
biological_process	GO:0006626	protein targeting to mitochondrion
biological_process	GO:0071806	protein transmembrane transport
cellular_component	GO:0005741	mitochondrial outer membrane
cellular_component	GO:0009941	chloroplast envelope
cellular_component	GO:0005742	mitochondrial outer membrane translocase complex
cellular_component	GO:0005774	vacuolar membrane
molecular_function	GO:0015450	P-P-bond-hydrolysis-driven protein transmembrane transporter activity