Spo06031.1 (mRNA) | SpinachBase

Overview


Name	Spo06031.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	Cytochrome b6-f complex subunit 7
Location	chr5 : 43748619 .. 43749014 (-)
Sequence length	396

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAACAGCAGCTGCATCAACTACTTTGTCCTCCGCAGCTGTACCGGCAATCTCAGGCAGCAGGGGTCAGAGGAAAATGAACAAAGTTGTTTACATGAGTGGAGTAAACTCCTATGGAGGTCTCAAGGCAAACAATGCTGTGCTCGGCCTCGGTCAGGCAGTATGTACCGAGCAGTGTTTCGCCAATGTTGTGAGTTCATTGAGGAGTACTGCTACCAAGAAAGGAAGATCAGGTGGTGGTGGTGGTGCTGGTGGTGGTGCCTTGACTTCTACCTGCAATGCTGCTGCTGAGATTTTCAGGATTGCTGCTGTTATGAATGGACTTACACTTGTTGGTGTTGCTATTGGTTTCGTTCTTCTGCGGATTGAGGCCACTGTAGAGGAGGCAGAGTAA

mRNA sequence

ATGGCAACAGCAGCTGCATCAACTACTTTGTCCTCCGCAGCTGTACCGGCAATCTCAGGCAGCAGGGGTCAGAGGAAAATGAACAAAGTTGTTTACATGAGTGGAGTAAACTCCTATGGAGGTCTCAAGGCAAACAATGCTGTGCTCGGCCTCGGTCAGGCAGTATGTACCGAGCAGTGTTTCGCCAATGTTGTGAGTTCATTGAGGAGTACTGCTACCAAGAAAGGAAGATCAGGTGGTGGTGGTGGTGCTGGTGGTGGTGCCTTGACTTCTACCTGCAATGCTGCTGCTGAGATTTTCAGGATTGCTGCTGTTATGAATGGACTTACACTTGTTGGTGTTGCTATTGGTTTCGTTCTTCTGCGGATTGAGGCCACTGTAGAGGAGGCAGAGTAA

Coding sequence (CDS)

ATGGCAACAGCAGCTGCATCAACTACTTTGTCCTCCGCAGCTGTACCGGCAATCTCAGGCAGCAGGGGTCAGAGGAAAATGAACAAAGTTGTTTACATGAGTGGAGTAAACTCCTATGGAGGTCTCAAGGCAAACAATGCTGTGCTCGGCCTCGGTCAGGCAGTATGTACCGAGCAGTGTTTCGCCAATGTTGTGAGTTCATTGAGGAGTACTGCTACCAAGAAAGGAAGATCAGGTGGTGGTGGTGGTGCTGGTGGTGGTGCCTTGACTTCTACCTGCAATGCTGCTGCTGAGATTTTCAGGATTGCTGCTGTTATGAATGGACTTACACTTGTTGGTGTTGCTATTGGTTTCGTTCTTCTGCGGATTGAGGCCACTGTAGAGGAGGCAGAGTAA

Protein sequence

MATAAASTTLSSAAVPAISGSRGQRKMNKVVYMSGVNSYGGLKANNAVLGLGQAVCTEQCFANVVSSLRSTATKKGRSGGGGGAGGGALTSTCNAAAEIFRIAAVMNGLTLVGVAIGFVLLRIEATVEEAE

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo06031	Spo06031	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo06031.1	Spo06031.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo06031.1.CDS.1	Spo06031.1.CDS.1	CDS

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo06031.1.exon.1	Spo06031.1.exon.1	exon

Homology

[Close]

BLAST of Spo06031.1 vs. NCBI nr
Match: gi|902227520|gb|KNA20733.1| (hypothetical protein SOVF_049660 [Spinacia oleracea])

HSP 1 Score: 235.3 bits (599), Expect = 5.900e-59
Identity = 131/131 (100.00%), Postives = 131/131 (100.00%), Query Frame = 1


		  
Query: 1   MATAAASTTLSSAAVPAISGSRGQRKMNKVVYMSGVNSYGGLKANNAVLGLGQAVCTEQC 60
           MATAAASTTLSSAAVPAISGSRGQRKMNKVVYMSGVNSYGGLKANNAVLGLGQAVCTEQC
Sbjct: 1   MATAAASTTLSSAAVPAISGSRGQRKMNKVVYMSGVNSYGGLKANNAVLGLGQAVCTEQC 60

Query: 61  FANVVSSLRSTATKKGRSGGGGGAGGGALTSTCNAAAEIFRIAAVMNGLTLVGVAIGFVL 120
           FANVVSSLRSTATKKGRSGGGGGAGGGALTSTCNAAAEIFRIAAVMNGLTLVGVAIGFVL
Sbjct: 61  FANVVSSLRSTATKKGRSGGGGGAGGGALTSTCNAAAEIFRIAAVMNGLTLVGVAIGFVL 120

Query: 121 LRIEATVEEAE 132
           LRIEATVEEAE
Sbjct: 121 LRIEATVEEAE 131

[Close]

BLAST of Spo06031.1 vs. NCBI nr
Match: gi|731314050|ref|XP_010684011.1| (PREDICTED: uncharacterized protein LOC104898612 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 193.7 bits (491), Expect = 2.000e-46
Identity = 112/134 (83.58%), Postives = 119/134 (88.81%), Query Frame = 1


		  
Query: 1   MATAAASTTLSSAAVPA---ISGSRGQRKMNKVVYMSGVNSYGGLKANNAVLGLGQAVCT 60
           MATAAASTTLSSAAVPA   ISGS+ QRKMNKVVYMSG+NSYGGLKANN V+GLG  VCT
Sbjct: 1   MATAAASTTLSSAAVPAAVTISGSKAQRKMNKVVYMSGLNSYGGLKANNTVVGLGNPVCT 60

Query: 61  EQCFANVVSSLRSTATKKGRSGGGGGAGGGALTSTCNAAAEIFRIAAVMNGLTLVGVAIG 120
           EQCFANVVSSLRST++KKGR G     GGGAL+STCNAAAEIFRIAAVMNGL LVGVA+G
Sbjct: 61  EQCFANVVSSLRSTSSKKGRLG-----GGGALSSTCNAAAEIFRIAAVMNGLVLVGVAVG 120

Query: 121 FVLLRIEATVEEAE 132
           FVLLRIEA VEEAE
Sbjct: 121 FVLLRIEAAVEEAE 129

[Close]

BLAST of Spo06031.1 vs. NCBI nr
Match: gi|720024963|ref|XP_010263800.1| (PREDICTED: uncharacterized protein LOC104601975 [Nelumbo nucifera])

HSP 1 Score: 138.7 bits (348), Expect = 7.500e-30
Identity = 82/130 (63.08%), Postives = 99/130 (76.15%), Query Frame = 1


		  
Query: 2   ATAAASTTLSSAAVPAISGSRGQRKMNKVVYMSGVNSYGGLKANNAVLGLGQAVCTEQCF 61
           A  +A+T  ++A V + +    Q+K N VVY+ G+NS+GGLKA+N+V  LG   CTEQ F
Sbjct: 6   AALSAATCTTAAIVSSSNRISTQKKRNNVVYIRGLNSFGGLKAHNSVAALGLTECTEQSF 65

Query: 62  ANVVSSLRSTATKKGRSGGGGGAGGGALTSTCNAAAEIFRIAAVMNGLTLVGVAIGFVLL 121
           A VVSSLRST+  KGR       GGGAL+STCNAAAEIFRIAAVMNGL LVGVA+GFVLL
Sbjct: 66  AKVVSSLRSTSKSKGR-------GGGALSSTCNAAAEIFRIAAVMNGLVLVGVAVGFVLL 125

Query: 122 RIEATVEEAE 132
           RIEA++EEAE
Sbjct: 126 RIEASLEEAE 128

[Close]

BLAST of Spo06031.1 vs. NCBI nr
Match: gi|719997300|ref|XP_010255052.1| (PREDICTED: uncharacterized protein LOC104595834 [Nelumbo nucifera])

HSP 1 Score: 132.1 bits (331), Expect = 7.000e-28
Identity = 78/130 (60.00%), Postives = 100/130 (76.92%), Query Frame = 1


		  
Query: 2   ATAAASTTLSSAAVPAISGSRGQRKMNKVVYMSGVNSYGGLKANNAVLGLGQAVCTEQCF 61
           + A ++ T +SA V +   S  +RK N VVY+ G+NS+GGLKA+N+V  LG  VCTEQ F
Sbjct: 5   SAALSAATCTSAGVMSGRNSTQKRKSN-VVYIGGLNSFGGLKAHNSVAALGLPVCTEQSF 64

Query: 62  ANVVSSLRSTATKKGRSGGGGGAGGGALTSTCNAAAEIFRIAAVMNGLTLVGVAIGFVLL 121
           A +VSSL+S++  KG+       GGGAL+STCNAAAEIF+IAA+MNGL LVGVA+GFVLL
Sbjct: 65  AKIVSSLKSSSQSKGK-------GGGALSSTCNAAAEIFKIAAIMNGLVLVGVAVGFVLL 124

Query: 122 RIEATVEEAE 132
           RIEA++EEAE
Sbjct: 125 RIEASLEEAE 126

[Close]

BLAST of Spo06031.1 vs. NCBI nr
Match: gi|659109813|ref|XP_008454894.1| (PREDICTED: uncharacterized protein LOC103495201 [Cucumis melo])

HSP 1 Score: 131.7 bits (330), Expect = 9.200e-28
Identity = 79/132 (59.85%), Postives = 102/132 (77.27%), Query Frame = 1


		  
Query: 1   MATAAAS-TTLSSAAVPAISGSRGQRKMNKVVYMSGVNSYGGLKANNAVLGLGQAVCTEQ 60
           MATA+A+ +T  S A P  +GSR QR+ N V Y++G+NS+GGLKA+N V+ LG  VC +Q
Sbjct: 1   MATASATLSTAMSTAAPIAAGSRRQRQTN-VHYITGLNSFGGLKAHNNVVSLGLPVCADQ 60

Query: 61  CFANVVSSLRSTATKKGRSGGGGGAGGGALTSTCNAAAEIFRIAAVMNGLTLVGVAIGFV 120
            FA +VSSL+  +  KG++G      GGAL+STCNAA EIFRIAA++NGL L+GVA+GFV
Sbjct: 61  SFAKIVSSLKYPSKGKGKNG------GGALSSTCNAAGEIFRIAAIINGLVLIGVAVGFV 120

Query: 121 LLRIEATVEEAE 132
           LLRIEA+VEEAE
Sbjct: 121 LLRIEASVEEAE 125

[Close]

BLAST of Spo06031.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RMM1_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_049660 PE=3 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 4.100e-59
Identity = 131/131 (100.00%), Postives = 131/131 (100.00%), Query Frame = 1


		  
Query: 1   MATAAASTTLSSAAVPAISGSRGQRKMNKVVYMSGVNSYGGLKANNAVLGLGQAVCTEQC 60
           MATAAASTTLSSAAVPAISGSRGQRKMNKVVYMSGVNSYGGLKANNAVLGLGQAVCTEQC
Sbjct: 1   MATAAASTTLSSAAVPAISGSRGQRKMNKVVYMSGVNSYGGLKANNAVLGLGQAVCTEQC 60

Query: 61  FANVVSSLRSTATKKGRSGGGGGAGGGALTSTCNAAAEIFRIAAVMNGLTLVGVAIGFVL 120
           FANVVSSLRSTATKKGRSGGGGGAGGGALTSTCNAAAEIFRIAAVMNGLTLVGVAIGFVL
Sbjct: 61  FANVVSSLRSTATKKGRSGGGGGAGGGALTSTCNAAAEIFRIAAVMNGLTLVGVAIGFVL 120

Query: 121 LRIEATVEEAE 132
           LRIEATVEEAE
Sbjct: 121 LRIEATVEEAE 131

[Close]

BLAST of Spo06031.1 vs. UniProtKB/TrEMBL
Match: A0A0J8CZJ8_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_1g012920 PE=3 SV=1)

HSP 1 Score: 193.7 bits (491), Expect = 1.400e-46
Identity = 112/134 (83.58%), Postives = 119/134 (88.81%), Query Frame = 1


		  
Query: 1   MATAAASTTLSSAAVPA---ISGSRGQRKMNKVVYMSGVNSYGGLKANNAVLGLGQAVCT 60
           MATAAASTTLSSAAVPA   ISGS+ QRKMNKVVYMSG+NSYGGLKANN V+GLG  VCT
Sbjct: 1   MATAAASTTLSSAAVPAAVTISGSKAQRKMNKVVYMSGLNSYGGLKANNTVVGLGNPVCT 60

Query: 61  EQCFANVVSSLRSTATKKGRSGGGGGAGGGALTSTCNAAAEIFRIAAVMNGLTLVGVAIG 120
           EQCFANVVSSLRST++KKGR G     GGGAL+STCNAAAEIFRIAAVMNGL LVGVA+G
Sbjct: 61  EQCFANVVSSLRSTSSKKGRLG-----GGGALSSTCNAAAEIFRIAAVMNGLVLVGVAVG 120

Query: 121 FVLLRIEATVEEAE 132
           FVLLRIEA VEEAE
Sbjct: 121 FVLLRIEAAVEEAE 129

[Close]

BLAST of Spo06031.1 vs. UniProtKB/TrEMBL
Match: E0CPD5_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_18s0001g11370 PE=3 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 6.400e-28
Identity = 79/131 (60.31%), Postives = 100/131 (76.34%), Query Frame = 1


		  
Query: 4   AAASTTLSSAAVPA---ISGSRGQRKMNKVVYMSGVNSYGGLKANNAVLGLGQAVCTEQC 63
           A AS  L  A V A    SGS+ Q+   KVVY++G+NS+GGLKA+N+V+ +G  +CTEQC
Sbjct: 2   ATASAILCPATVTARVRSSGSKTQKGEKKVVYVAGLNSFGGLKAHNSVVAMGVPMCTEQC 61

Query: 64  FANVVSSLRSTATKKGRSGGGGGAGGGALTSTCNAAAEIFRIAAVMNGLTLVGVAIGFVL 123
           FANVVSSL++    KGR GGG      AL+STC+AA+EIFRIAA+MN L L+GVA+GFVL
Sbjct: 62  FANVVSSLKA----KGRGGGG------ALSSTCSAASEIFRIAAIMNALVLIGVAVGFVL 121

Query: 124 LRIEATVEEAE 132
           LRIEA+VEE+E
Sbjct: 122 LRIEASVEESE 122

[Close]

BLAST of Spo06031.1 vs. UniProtKB/TrEMBL
Match: B9R7K1_RICCO (Plastoquinol-plastocyanin reductase, putative OS=Ricinus communis GN=RCOM_1592620 PE=3 SV=1)

HSP 1 Score: 131.0 bits (328), Expect = 1.100e-27
Identity = 78/132 (59.09%), Postives = 101/132 (76.52%), Query Frame = 1


		  
Query: 1   MATAAASTTLSSAAVPAIS-GSRGQRKMNKVVYMSGVNSYGGLKANNAVLGLGQAVCTEQ 60
           MAT AA+ T +     A+S G++ Q++M+KVVY+ G+NS+GGLKA+N+V+ LG  VCTEQ
Sbjct: 1   MATTAATFTPARINGAAVSFGNKPQKRMDKVVYIRGLNSFGGLKAHNSVVSLGMPVCTEQ 60

Query: 61  CFANVVSSLRSTATKKGRSGGGGGAGGGALTSTCNAAAEIFRIAAVMNGLTLVGVAIGFV 120
           CFAN VSS+++ A     +  G G GGGAL+S C+   EIF+IAA+MNGL LVGVAIGFV
Sbjct: 61  CFANFVSSIKAAAA----ASNGKGKGGGALSSKCSKIGEIFQIAAIMNGLVLVGVAIGFV 120

Query: 121 LLRIEATVEEAE 132
           LLRIEA VEE+E
Sbjct: 121 LLRIEAFVEESE 128

[Close]

BLAST of Spo06031.1 vs. UniProtKB/TrEMBL
Match: A0A166GGY9_DAUCA (Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_001610 PE=4 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 2.400e-27
Identity = 81/132 (61.36%), Postives = 92/132 (69.70%), Query Frame = 1


		  
Query: 5   AASTTLSSAAVPAISGS-----RGQRKMNKVVYMSGVNSYGGLKANNAVLGLGQAVCTEQ 64
           AAS TL  A   AIS +     R  +K   V Y+SG+NS+GGLKA N+V+ LG  VCTEQ
Sbjct: 2   AASATLCPAKFTAISAAVACPTRASQKRPNVKYISGLNSFGGLKAQNSVVSLGLPVCTEQ 61

Query: 65  CFANVVSSLRSTATKKGRSGGGGGAGGGALTSTCNAAAEIFRIAAVMNGLTLVGVAIGFV 124
            FAN  SSL+    + G        GGGALTSTCNA AEIFRIAAV+NGLTLVGVA+GFV
Sbjct: 62  SFANFASSLKYPNQRNG--------GGGALTSTCNAVAEIFRIAAVINGLTLVGVAVGFV 121

Query: 125 LLRIEATVEEAE 132
           LLRIEA VEEAE
Sbjct: 122 LLRIEAAVEEAE 125

[Close]

BLAST of Spo06031.1 vs. ExPASy Swiss-Prot
Match: PETM_SPIOL (Cytochrome b6-f complex subunit 7 (Fragment) OS=Spinacia oleracea GN=petM PE=1 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 3.900e-10
Identity = 36/36 (100.00%), Postives = 36/36 (100.00%), Query Frame = 1


		  
Query: 94  NAAAEIFRIAAVMNGLTLVGVAIGFVLLRIEATVEE 130
           NAAAEIFRIAAVMNGLTLVGVAIGFVLLRIEATVEE
Sbjct: 1   NAAAEIFRIAAVMNGLTLVGVAIGFVLLRIEATVEE 36

[Close]

BLAST of Spo06031.1 vs. TAIR (Arabidopsis)
Match: AT2G26500.2 (cytochrome b6f complex subunit (petM), putative)

HSP 1 Score: 121.7 bits (304), Expect = 3.300e-28
Identity = 77/134 (57.46%), Postives = 95/134 (70.90%), Query Frame = 1


		  
Query: 1   MATAAASTTLS--SAAVPAISGSRGQRKMNKVVYMSGVNSYGGLKA-NNAVLGLGQAVCT 60
           MATAAA   +S   + + + SG   ++   KV Y++G+NSYGGLKA NN V+ +G  +CT
Sbjct: 1   MATAAAPAVISWTRSGIVSKSGQTQKKSEMKVSYITGLNSYGGLKAQNNKVVSMGSPLCT 60

Query: 61  EQCFANVVSSLRSTATKKGRSGGGGGAGGGALTSTCNAAAEIFRIAAVMNGLTLVGVAIG 120
           EQCFANVV SL      KGR G      GGAL++TCNA  EIF+IAA+MN LTLVGVA+G
Sbjct: 61  EQCFANVVMSL------KGRRGN-----GGALSTTCNAVGEIFKIAAIMNALTLVGVAVG 120

Query: 121 FVLLRIEATVEEAE 132
           FVLLRIE +VEEAE
Sbjct: 121 FVLLRIETSVEEAE 123

The following BLAST results are available for this feature:

BLAST of Spo06031.1 vs. NCBI nr
BLAST of Spo06031.1 vs. UniProtKB/TrEMBL
BLAST of Spo06031.1 vs. ExPASy Swiss-Prot
BLAST of Spo06031.1 vs. TAIR (Arabidopsis)

BLAST of Spo06031.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902227520\|gb\|KNA20733.1\|	5.9e-59	100.	hypothetical protein SOVF_0496...	[more]
gi\|731314050\|ref\|XP_010684011.1\|	2.0e-46	83.5	PREDICTED: uncharacterized pro...	[more]
gi\|720024963\|ref\|XP_010263800.1\|	7.5e-30	63.0	PREDICTED: uncharacterized pro...	[more]
gi\|719997300\|ref\|XP_010255052.1\|	7.0e-28	60.0	PREDICTED: uncharacterized pro...	[more]
gi\|659109813\|ref\|XP_008454894.1\|	9.2e-28	59.8	PREDICTED: uncharacterized pro...	[more]

BLAST of Spo06031.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9RMM1_SPIOL	4.1e-59	100.	Uncharacterized protein OS=Spi...	[more]
A0A0J8CZJ8_BETVU	1.4e-46	83.5	Uncharacterized protein OS=Bet...	[more]
E0CPD5_VITVI	6.4e-28	60.3	Putative uncharacterized prote...	[more]
B9R7K1_RICCO	1.1e-27	59.0	Plastoquinol-plastocyanin redu...	[more]
A0A166GGY9_DAUCA	2.4e-27	61.3	Uncharacterized protein OS=Dau...	[more]

BLAST of Spo06031.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 1


Match Name	E-value	Identity	Description
PETM_SPIOL	3.9e-10	100.	Cytochrome b6-f complex subuni...	[more]

BLAST of Spo06031.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 1


Match Name	E-value	Identity	Description
AT2G26500.2	3.3e-28	57.4	cytochrome b6f complex subunit...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012595	PetM of cytochrome b6/f complex subunit 7	HAMAP	MF_00396	Cytb6_f_PetM	coord: 95..128 score: 1
IPR012595	PetM of cytochrome b6/f complex subunit 7	PFAM	PF08041	PetM	coord: 97..124 score: 1.9
None	No IPR available	PANTHER	PTHR34951	FAMILY NOT NAMED	coord: 1..131 score: 2.7
None	No IPR available	unknown	SSF103441	PetM subunit of the cytochrome b6f complex	coord: 96..128 score: 4.18

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0009512	cytochrome b6f complex
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0016491	oxidoreductase activity