Spo06145.1 (mRNA) | SpinachBase

Overview


Name	Spo06145.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	Ubiquitin-conjugating enzyme E2 32
Location	Super_scaffold_65 : 36799 .. 39838 (-)
Sequence length	1531

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATCACCAAACGAAAACCGTGTTCGTCTCCCACTCTAAAATTTCAATTTCCAGATATTCCTCTTTTTCCCCTCTCTCTCTCATCAATCGTCATTCTTACATACCACCCACATAAAAAGAATATCAGCACCACTACCGGCGATCTCGTCGGAATATTCTTCGGGAAATCTCCAATCATGGCGGCTACCGACAAGTATAACCTAAAGAATCCGGCGGTGAAGCGGATCCTTATGGAAGTCAAAGAGATGCAGACCAATCCTTCCGACGATTTCATGAGTGTTCCTCTCGAGGTATCTCTTTCTCTTTCTCTCTCCTCTTTTTGTTTTCGTATTTCTTGTACCTTTTTTGTTATTTTATTCCAATTTGATTTCATAGATTGATTTCTAGCTATGTTGATTAAGGTGCTATTTTTTTTATATTATTTGTTATTGTTTGCGATTTTGTTTTGATGTTCTTCTTGAGCTGTTTTTTCTCTACTGAATTTTCTGCAATTACTGAAATTTTTCGAAGTTGTATGGTTAATGATGTGTTATGTAATCTGATCATGGATCTGTTGAGCTTGAATTTGATCTCGATTGTGTTCTATAGGGTTTCTACTGCCGTTTTCTCCTTTATTTGCAATTTCTAGTTGGATATGTTCATTTGGTACATAACTTTTGCTTGAATAATGAAAATATGGCGGTTATAGATTGGCATGATGGCGTATTTTTTGTTTTGTGCTGAAATAATATGGATGCATTTTCTAGAATTTCTGGAATTGCGTTTTAAACTTCGTATATGCGGATTATTTTGATTTTGTTACCAAAATGTGCACAATATGTAGAATTTCAGCAACTTTCTTTTGCAATTTCGCCGTAGAAATAAAGAGTTAGTGTATGTGTACTATAGTATAGTGTTGCTCAATTACCATAAATGTATTACTTATCTCGACTTTAGATGGGGAGTACAGAGTTAAAGCCTGTAGAGGCGAAATAGAAATGCTTATCTCGTTGAGTTCGACTTCATTCAATTGGAGGTGGTTTTGAAGCATTGTTTTTGTTGATGATTGAGTTTTCTGCCGTTTACATTTCAGGAGAACATATTTGAATGGCAATTTGCAATTCGGGGACCCCAAGATACTGAATTTGAGGGTGGTATATATCATGGAAGGATTCAGCTGCCAGCAGAATATCCTTTTAAGCCTCCTTCTTTCATGTTGTTGACGGTAATCACTTTTACTGAAAACTCTGTCTATTATTCCTTGTCAGACCAATTTGAAAGTATTGTTACTTTTAAACCTGTTTGCAGCCCAATGGTCGTTTTGAGACACAGACAAAAATATGTTTGAGCATATCAAACCATCATCCAGAGCACTGGCAACCATCTTGGAGTGGTAAGTTGGACTTTTCTTGTTCCTTGGACATGATTCAAAATCCAAATAATATCTGTACAAGTTGTATTTGTTGGCATCATTGCATGAAAACTTTAAGCGTGCCTTTCTTTGTGTCTTTGTTGCATGATTCTTGAATGCTGTAAAATCGGTTCTGAGATTGATGTTATACAAATAATACAACGCACAGTGTGAACTGTTAAGCCTAGGGTTCTGGGGGCAGTGTTTGATATTGCAAATTCTTGATCAGAATTCCTTAAGGGAATGAACGTATGGGTATACATTAAAGAGTTGGGTTTTTTACCTGGGAGCATTGGAGTTTGGACTTCTGAAGTTACATTTTGAAATAATTAGCAGAAAAAAATGAGATGTACGCATGTGTCACTAGGAGATCTGATGTAGTAGTCTTTAGATATCCTGCTTAGGAGTTTTTCTTTCGGTCACTGAACCTGAAAATGAGTATAATCAATTTTATTCGTTGGCCTATGTTTTTATTAAATCCTATTTACTTTTTTCCATTACCAACATTAATGATTCATTCAATGGTGGTGGCAGTTCGTACTGCATTGGTGGCCCTCATTGCTTTCATGCCGACAGCCCCTAATGGTGCTTTAGGATCTTTAGACTACAAGAAGGAAGAAAGGCGTGACCTAGCTATTAGATCTCGTGAAGCATCACCAAAATTCGGAACTCCTGAACGTCAAAAGGTGATTGATGAGGTCAGTCTTCTTCACTCTTGAGTAACCTGTCTTCCTCTTTATCAAGTTACGTACAACTGCCTACTTAATCTCAAAACTTATCGACTTTACAGATTCATGAATACGTGCTTAGTAAGGCTCCACCAGTTCCTGAAAGCCTAAAGCAGACTTCTGAAGAACACCAAAGTGAAAAGGAGGCTGAAGAAGCTGAGGGAAGTGAAAATGCTGGAAGATCAGCTGATTCTGTAGAACCTGAAAACGAAGGAGGTGAGAGAATTACTGAGGAACCTCAAGCGGCTGTTGAGAACGCTGATGCTGTGGAACAAAGGGAAGCTATTCCTAGACAAGTTAGAGCTGGAGTTTCTAACCAACAGCTTCAACACAGGCCTGCACAAAGGTCAACTGATGATCGCTTGCTCACATGGGCAGCTGTGGGATTGACTGTTGCCATAGTTTTTCTTTTACTGAAGAAGCTCATGAAATCCAGTGGTTATGACGCTATTTTTTTGAGCGAATCGTAGATATGGTAGATATTTATAGGGCAGGACAAACAAAACATGTCTTGTATTTGTGAGAAAATATTCAAGTATATGGTGTAATATATTGCGTAGGTCTTAATCAAGCTGTTTGATTATAATAGCATTACTAGTAAAACATATGTAGTGTGATGATTGGGGTTATTCCTTCCCTGGAATATTAGTATTGTCTTACATACGAGTAAGTCAGAAGCTTGGTAAACATTGTCTATGGACCTCGCTGATATATTTTGGTAGACTCACGGTGGATTATACATGTAACTGATGTAAACAACATTCGTATGCTCAAACTTGTGGATATATTCTGTATTTTGTCTATAGAACTCTCTATGGTCTGAGAGTCTCACAACAGAATTTTCAAAGGACAAGAATTTCAATAAAATTTCGAGTTTTAAAGTGCAGGAGTCCTCTGAAGAGGAGAGAGTGCGCATGGCTAACA

mRNA sequence

ATCACCAAACGAAAACCGTGTTCGTCTCCCACTCTAAAATTTCAATTTCCAGATATTCCTCTTTTTCCCCTCTCTCTCTCATCAATCGTCATTCTTACATACCACCCACATAAAAAGAATATCAGCACCACTACCGGCGATCTCGTCGGAATATTCTTCGGGAAATCTCCAATCATGGCGGCTACCGACAAGTATAACCTAAAGAATCCGGCGGTGAAGCGGATCCTTATGGAAGTCAAAGAGATGCAGACCAATCCTTCCGACGATTTCATGAGTGTTCCTCTCGAGGAGAACATATTTGAATGGCAATTTGCAATTCGGGGACCCCAAGATACTGAATTTGAGGGTGGTATATATCATGGAAGGATTCAGCTGCCAGCAGAATATCCTTTTAAGCCTCCTTCTTTCATGTTGTTGACGCCCAATGGTCGTTTTGAGACACAGACAAAAATATGTTTGAGCATATCAAACCATCATCCAGAGCACTGGCAACCATCTTGGAGTGTTCGTACTGCATTGGTGGCCCTCATTGCTTTCATGCCGACAGCCCCTAATGGTGCTTTAGGATCTTTAGACTACAAGAAGGAAGAAAGGCGTGACCTAGCTATTAGATCTCGTGAAGCATCACCAAAATTCGGAACTCCTGAACGTCAAAAGGTGATTGATGAGATTCATGAATACGTGCTTAGTAAGGCTCCACCAGTTCCTGAAAGCCTAAAGCAGACTTCTGAAGAACACCAAAGTGAAAAGGAGGCTGAAGAAGCTGAGGGAAGTGAAAATGCTGGAAGATCAGCTGATTCTGTAGAACCTGAAAACGAAGGAGGTGAGAGAATTACTGAGGAACCTCAAGCGGCTGTTGAGAACGCTGATGCTGTGGAACAAAGGGAAGCTATTCCTAGACAAGTTAGAGCTGGAGTTTCTAACCAACAGCTTCAACACAGGCCTGCACAAAGGTCAACTGATGATCGCTTGCTCACATGGGCAGCTGTGGGATTGACTGTTGCCATAGTTTTTCTTTTACTGAAGAAGCTCATGAAATCCAGTGGTTATGACGCTATTTTTTTGAGCGAATCGTAGATATGGTAGATATTTATAGGGCAGGACAAACAAAACATGTCTTGTATTTGTGAGAAAATATTCAAGTATATGGTGTAATATATTGCGTAGGTCTTAATCAAGCTGTTTGATTATAATAGCATTACTAGTAAAACATATGTAGTGTGATGATTGGGGTTATTCCTTCCCTGGAATATTAGTATTGTCTTACATACGAGTAAGTCAGAAGCTTGGTAAACATTGTCTATGGACCTCGCTGATATATTTTGGTAGACTCACGGTGGATTATACATGTAACTGATGTAAACAACATTCGTATGCTCAAACTTGTGGATATATTCTGTATTTTGTCTATAGAACTCTCTATGGTCTGAGAGTCTCACAACAGAATTTTCAAAGGACAAGAATTTCAATAAAATTTCGAGTTTTAAAGTGCAGGAGTCCTCTGAAGAGGAGAGAGTGCGCATGGCTAACA

Coding sequence (CDS)

ATGGCGGCTACCGACAAGTATAACCTAAAGAATCCGGCGGTGAAGCGGATCCTTATGGAAGTCAAAGAGATGCAGACCAATCCTTCCGACGATTTCATGAGTGTTCCTCTCGAGGAGAACATATTTGAATGGCAATTTGCAATTCGGGGACCCCAAGATACTGAATTTGAGGGTGGTATATATCATGGAAGGATTCAGCTGCCAGCAGAATATCCTTTTAAGCCTCCTTCTTTCATGTTGTTGACGCCCAATGGTCGTTTTGAGACACAGACAAAAATATGTTTGAGCATATCAAACCATCATCCAGAGCACTGGCAACCATCTTGGAGTGTTCGTACTGCATTGGTGGCCCTCATTGCTTTCATGCCGACAGCCCCTAATGGTGCTTTAGGATCTTTAGACTACAAGAAGGAAGAAAGGCGTGACCTAGCTATTAGATCTCGTGAAGCATCACCAAAATTCGGAACTCCTGAACGTCAAAAGGTGATTGATGAGATTCATGAATACGTGCTTAGTAAGGCTCCACCAGTTCCTGAAAGCCTAAAGCAGACTTCTGAAGAACACCAAAGTGAAAAGGAGGCTGAAGAAGCTGAGGGAAGTGAAAATGCTGGAAGATCAGCTGATTCTGTAGAACCTGAAAACGAAGGAGGTGAGAGAATTACTGAGGAACCTCAAGCGGCTGTTGAGAACGCTGATGCTGTGGAACAAAGGGAAGCTATTCCTAGACAAGTTAGAGCTGGAGTTTCTAACCAACAGCTTCAACACAGGCCTGCACAAAGGTCAACTGATGATCGCTTGCTCACATGGGCAGCTGTGGGATTGACTGTTGCCATAGTTTTTCTTTTACTGAAGAAGCTCATGAAATCCAGTGGTTATGACGCTATTTTTTTGAGCGAATCGTAG

Protein sequence

MAATDKYNLKNPAVKRILMEVKEMQTNPSDDFMSVPLEENIFEWQFAIRGPQDTEFEGGIYHGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFMPTAPNGALGSLDYKKEERRDLAIRSREASPKFGTPERQKVIDEIHEYVLSKAPPVPESLKQTSEEHQSEKEAEEAEGSENAGRSADSVEPENEGGERITEEPQAAVENADAVEQREAIPRQVRAGVSNQQLQHRPAQRSTDDRLLTWAAVGLTVAIVFLLLKKLMKSSGYDAIFLSES

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo06145	Spo06145	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo06145.1	Spo06145.1-protein	polypeptide

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo06145.1.utr5p.1	Spo06145.1.utr5p.1	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo06145.1.CDS.5	Spo06145.1.CDS.5	CDS
Spo06145.1.CDS.4	Spo06145.1.CDS.4	CDS
Spo06145.1.CDS.3	Spo06145.1.CDS.3	CDS
Spo06145.1.CDS.2	Spo06145.1.CDS.2	CDS
Spo06145.1.CDS.1	Spo06145.1.CDS.1	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo06145.1.utr3p.1	Spo06145.1.utr3p.1	three_prime_UTR

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo06145.1.exon.5	Spo06145.1.exon.5	exon
Spo06145.1.exon.4	Spo06145.1.exon.4	exon
Spo06145.1.exon.3	Spo06145.1.exon.3	exon
Spo06145.1.exon.2	Spo06145.1.exon.2	exon
Spo06145.1.exon.1	Spo06145.1.exon.1	exon

Homology

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BLAST of Spo06145.1 vs. NCBI nr
Match: gi|902179648|gb|KNA09393.1| (hypothetical protein SOVF_153830 [Spinacia oleracea])

HSP 1 Score: 585.9 bits (1509), Expect = 4.100e-164
Identity = 300/300 (100.00%), Postives = 300/300 (100.00%), Query Frame = 1


		  
Query: 1   MAATDKYNLKNPAVKRILMEVKEMQTNPSDDFMSVPLEENIFEWQFAIRGPQDTEFEGGI 60
           MAATDKYNLKNPAVKRILMEVKEMQTNPSDDFMSVPLEENIFEWQFAIRGPQDTEFEGGI
Sbjct: 1   MAATDKYNLKNPAVKRILMEVKEMQTNPSDDFMSVPLEENIFEWQFAIRGPQDTEFEGGI 60

Query: 61  YHGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIA 120
           YHGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIA
Sbjct: 61  YHGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIA 120

Query: 121 FMPTAPNGALGSLDYKKEERRDLAIRSREASPKFGTPERQKVIDEIHEYVLSKAPPVPES 180
           FMPTAPNGALGSLDYKKEERRDLAIRSREASPKFGTPERQKVIDEIHEYVLSKAPPVPES
Sbjct: 121 FMPTAPNGALGSLDYKKEERRDLAIRSREASPKFGTPERQKVIDEIHEYVLSKAPPVPES 180

Query: 181 LKQTSEEHQSEKEAEEAEGSENAGRSADSVEPENEGGERITEEPQAAVENADAVEQREAI 240
           LKQTSEEHQSEKEAEEAEGSENAGRSADSVEPENEGGERITEEPQAAVENADAVEQREAI
Sbjct: 181 LKQTSEEHQSEKEAEEAEGSENAGRSADSVEPENEGGERITEEPQAAVENADAVEQREAI 240

Query: 241 PRQVRAGVSNQQLQHRPAQRSTDDRLLTWAAVGLTVAIVFLLLKKLMKSSGYDAIFLSES 300
           PRQVRAGVSNQQLQHRPAQRSTDDRLLTWAAVGLTVAIVFLLLKKLMKSSGYDAIFLSES
Sbjct: 241 PRQVRAGVSNQQLQHRPAQRSTDDRLLTWAAVGLTVAIVFLLLKKLMKSSGYDAIFLSES 300

[Close]

BLAST of Spo06145.1 vs. NCBI nr
Match: gi|731322080|ref|XP_010671695.1| (PREDICTED: ubiquitin-conjugating enzyme E2 32 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 491.9 bits (1265), Expect = 8.000e-136
Identity = 256/303 (84.49%), Postives = 272/303 (89.77%), Query Frame = 1


		  
Query: 1   MAATDKYNLKNPAVKRILMEVKEMQTNPSDDFMSVPLEENIFEWQFAIRGPQDTEFEGGI 60
           MAATDKYNLKNPAVKRIL EVKEMQ+NPSDDFMS+PLEENIFEWQFAIRGP +TEFEGGI
Sbjct: 1   MAATDKYNLKNPAVKRILQEVKEMQSNPSDDFMSLPLEENIFEWQFAIRGPGETEFEGGI 60

Query: 61  YHGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIA 120
           YHGRIQLPA+YPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIA
Sbjct: 61  YHGRIQLPADYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIA 120

Query: 121 FMPTAPNGALGSLDYKKEERRDLAIRSREASPKFGTPERQKVIDEIHEYVLSKAPPVPES 180
           FMPT PNGALGSLDYKKEERR+LAIRSRE SPKFGTP+RQKVIDEIH Y+LSKAPPVPE 
Sbjct: 121 FMPTPPNGALGSLDYKKEERRELAIRSREGSPKFGTPDRQKVIDEIHAYMLSKAPPVPEI 180

Query: 181 LKQTSEEHQSEKEAEEAEGSENAGRSADSVEPENEGGERITEEPQAAVENADAVEQREAI 240
           LKQ  +E  +E+EA EAEGSENAGRSA +VE  N  GE I EEPQAAV NA+ V+ REA 
Sbjct: 181 LKQAPDEQSAEREA-EAEGSENAGRSATAVESSNAEGEIINEEPQAAVANANRVDHREAN 240

Query: 241 PRQVRAGVSNQQLQHRPA---QRSTDDRLLTWAAVGLTVAIVFLLLKKLMKSSGYDAIFL 300
           P  VRAG+ NQQL+HRPA   QRS DDRLLTWAAVGLTVAIVFLLLKKL+KS GY AIFL
Sbjct: 241 PGLVRAGILNQQLEHRPAPRIQRSNDDRLLTWAAVGLTVAIVFLLLKKLVKSGGYGAIFL 300

[Close]

BLAST of Spo06145.1 vs. NCBI nr
Match: gi|703103678|ref|XP_010097791.1| (Ubiquitin-conjugating enzyme E2 32 [Morus notabilis])

HSP 1 Score: 407.1 bits (1045), Expect = 2.600e-110
Identity = 216/304 (71.05%), Postives = 246/304 (80.92%), Query Frame = 1


		  
Query: 3   ATDKYNLKNPAVKRILMEVKEMQTNPSDDFMSVPLEENIFEWQFAIRGPQDTEFEGGIYH 62
           A DKYNLKNPAVKRIL E+KEMQ+N SDDFMS+PLEENIFEWQFAIRGP+DTEFEGGIYH
Sbjct: 2   AEDKYNLKNPAVKRILQEIKEMQSNSSDDFMSLPLEENIFEWQFAIRGPRDTEFEGGIYH 61

Query: 63  GRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFM 122
           GRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFM
Sbjct: 62  GRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFM 121

Query: 123 PTAPNGALGSLDYKKEERRDLAIRSREASPKFGTPERQKVIDEIHEYVLSKAPPVPESLK 182
           PT+PNGALGSLDYKKEERR LA++SREASPKFGTPERQK+IDE+HEY+LSKAPPVP+   
Sbjct: 122 PTSPNGALGSLDYKKEERRALAVKSREASPKFGTPERQKLIDEVHEYMLSKAPPVPQLCP 181

Query: 183 QTSEEHQSEK--EAEEAEGSENA--GRSADSVEPENEGGERITEEPQAAVENADAVEQRE 242
             + E Q  K  E +   GS+N+   ++A+ + P    GER+ EE Q +  NA+    RE
Sbjct: 182 SETPEEQPTKIEEGDAQAGSQNSDVAQAAEGL-PNPSVGERVVEEHQESPANANPRLARE 241

Query: 243 AIPRQVRAGVSNQQLQHRP--AQRSTDDRLLTWAAVGLTVAIVFLLLKKLMKSSGYDAIF 301
             P +V +  SNQ LQ +   AQR  DDRL TWAAVGLT+AIV LLLKK M + G+ A+F
Sbjct: 242 RAPSEVHS--SNQVLQRQEVRAQRPADDRLFTWAAVGLTIAIVVLLLKKFMNAGGHGAVF 301

[Close]

BLAST of Spo06145.1 vs. NCBI nr
Match: gi|590661821|ref|XP_007035779.1| (Ubiquitin-conjugating enzyme 32 [Theobroma cacao])

HSP 1 Score: 406.4 bits (1043), Expect = 4.400e-110
Identity = 216/304 (71.05%), Postives = 245/304 (80.59%), Query Frame = 1


		  
Query: 3   ATDKYNLKNPAVKRILMEVKEMQTNPSDDFMSVPLEENIFEWQFAIRGPQDTEFEGGIYH 62
           A DKYNLKNPAVKRIL EVKEMQ+NPSDDFMS+PLEENIFEWQFAIRGP+++EFEGGIYH
Sbjct: 2   AEDKYNLKNPAVKRILQEVKEMQSNPSDDFMSLPLEENIFEWQFAIRGPRESEFEGGIYH 61

Query: 63  GRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFM 122
           GRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFM
Sbjct: 62  GRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFM 121

Query: 123 PTAPNGALGSLDYKKEERRDLAIRSREASPKFGTPERQKVIDEIHEYVLSKAPPVPE-SL 182
           PT+PNGALGSLDYKKEERR LAI+SREA PKFG PERQK+IDEIHEY+LSKAPPVP+ S 
Sbjct: 122 PTSPNGALGSLDYKKEERRALAIKSREAPPKFGNPERQKLIDEIHEYMLSKAPPVPQLSP 181

Query: 183 KQTSEEHQSEKE-AEEAEGSENAGRSADSVEPENEGGERITEEPQAAVENADAVEQREAI 242
            Q SEEH  +++   +A   ++   +     P    G+R+ EE   A  NA+       +
Sbjct: 182 SQASEEHSMDRDGVTQANQQDSVSMAGGDGLPNQAVGDRVVEEEPVAPANANPAPAEIRV 241

Query: 243 PRQVRA-GVSNQQLQHRP---AQRSTDDRLLTWAAVGLTVAIVFLLLKKLMKSSGYDAIF 301
            R+V A G SNQ LQ RP    Q+S DDRL TWAAVGLT+AI+ LLLKK +KSSG+ A+F
Sbjct: 242 VREVPARGPSNQPLQ-RPELRVQKSADDRLFTWAAVGLTIAILVLLLKKFLKSSGHGAVF 301

[Close]

BLAST of Spo06145.1 vs. NCBI nr
Match: gi|255556356|ref|XP_002519212.1| (PREDICTED: ubiquitin-conjugating enzyme E2 32 [Ricinus communis])

HSP 1 Score: 402.1 bits (1032), Expect = 8.300e-109
Identity = 215/304 (70.72%), Postives = 242/304 (79.61%), Query Frame = 1


		  
Query: 3   ATDKYNLKNPAVKRILMEVKEMQTNPSDDFMSVPLEENIFEWQFAIRGPQDTEFEGGIYH 62
           A DKYNLKNPAVKRIL EVKEMQ+NPSDDFMS+PLEENIFEWQFAIRGP DTEFEGGIYH
Sbjct: 2   AEDKYNLKNPAVKRILQEVKEMQSNPSDDFMSMPLEENIFEWQFAIRGPCDTEFEGGIYH 61

Query: 63  GRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFM 122
           GRIQLP+EYPFKPPSF+LLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFM
Sbjct: 62  GRIQLPSEYPFKPPSFLLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFM 121

Query: 123 PTAPNGALGSLDYKKEERRDLAIRSREASPKFGTPERQKVIDEIHEYVLSKAPPVPE-SL 182
           PT PNGALGSLDYKKEERR LAI+SREA+P+FGTPERQK+IDEIH+Y+LSKAPPVP+ S 
Sbjct: 122 PTNPNGALGSLDYKKEERRALAIKSREAAPRFGTPERQKLIDEIHQYMLSKAPPVPQLSP 181

Query: 183 KQTSEEHQSEKEAE-----EAEGSENAGRSADSVEPENEGGERITEEPQAAVENADAVEQ 242
            Q SEEH + KE E     + +G+  A     +V  +   G+RI EE   A  N +    
Sbjct: 182 SQDSEEHHTGKEDEAHAILQEQGAVAANLPNLAVSEQLAVGDRIVEEVPEAPVNVNPGAT 241

Query: 243 REAIPRQVRAGVSNQQLQHRP---AQRSTDDRLLTWAAVGLTVAIVFLLLKKLMKSSGYD 298
                 ++ A  S+ QL HRP    QR  DDRL TWAAVGLT+AIV LLLKK +KSSG+ 
Sbjct: 242 GVRTSTEIPARASSDQLLHRPETRVQRPADDRLFTWAAVGLTIAIVVLLLKKFLKSSGHG 301

[Close]

BLAST of Spo06145.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QRS7_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_153830 PE=4 SV=1)

HSP 1 Score: 585.9 bits (1509), Expect = 2.800e-164
Identity = 300/300 (100.00%), Postives = 300/300 (100.00%), Query Frame = 1


		  
Query: 1   MAATDKYNLKNPAVKRILMEVKEMQTNPSDDFMSVPLEENIFEWQFAIRGPQDTEFEGGI 60
           MAATDKYNLKNPAVKRILMEVKEMQTNPSDDFMSVPLEENIFEWQFAIRGPQDTEFEGGI
Sbjct: 1   MAATDKYNLKNPAVKRILMEVKEMQTNPSDDFMSVPLEENIFEWQFAIRGPQDTEFEGGI 60

Query: 61  YHGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIA 120
           YHGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIA
Sbjct: 61  YHGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIA 120

Query: 121 FMPTAPNGALGSLDYKKEERRDLAIRSREASPKFGTPERQKVIDEIHEYVLSKAPPVPES 180
           FMPTAPNGALGSLDYKKEERRDLAIRSREASPKFGTPERQKVIDEIHEYVLSKAPPVPES
Sbjct: 121 FMPTAPNGALGSLDYKKEERRDLAIRSREASPKFGTPERQKVIDEIHEYVLSKAPPVPES 180

Query: 181 LKQTSEEHQSEKEAEEAEGSENAGRSADSVEPENEGGERITEEPQAAVENADAVEQREAI 240
           LKQTSEEHQSEKEAEEAEGSENAGRSADSVEPENEGGERITEEPQAAVENADAVEQREAI
Sbjct: 181 LKQTSEEHQSEKEAEEAEGSENAGRSADSVEPENEGGERITEEPQAAVENADAVEQREAI 240

Query: 241 PRQVRAGVSNQQLQHRPAQRSTDDRLLTWAAVGLTVAIVFLLLKKLMKSSGYDAIFLSES 300
           PRQVRAGVSNQQLQHRPAQRSTDDRLLTWAAVGLTVAIVFLLLKKLMKSSGYDAIFLSES
Sbjct: 241 PRQVRAGVSNQQLQHRPAQRSTDDRLLTWAAVGLTVAIVFLLLKKLMKSSGYDAIFLSES 300

[Close]

BLAST of Spo06145.1 vs. UniProtKB/TrEMBL
Match: A0A0J8CR27_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_3g053960 PE=4 SV=1)

HSP 1 Score: 491.9 bits (1265), Expect = 5.600e-136
Identity = 256/303 (84.49%), Postives = 272/303 (89.77%), Query Frame = 1


		  
Query: 1   MAATDKYNLKNPAVKRILMEVKEMQTNPSDDFMSVPLEENIFEWQFAIRGPQDTEFEGGI 60
           MAATDKYNLKNPAVKRIL EVKEMQ+NPSDDFMS+PLEENIFEWQFAIRGP +TEFEGGI
Sbjct: 1   MAATDKYNLKNPAVKRILQEVKEMQSNPSDDFMSLPLEENIFEWQFAIRGPGETEFEGGI 60

Query: 61  YHGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIA 120
           YHGRIQLPA+YPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIA
Sbjct: 61  YHGRIQLPADYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIA 120

Query: 121 FMPTAPNGALGSLDYKKEERRDLAIRSREASPKFGTPERQKVIDEIHEYVLSKAPPVPES 180
           FMPT PNGALGSLDYKKEERR+LAIRSRE SPKFGTP+RQKVIDEIH Y+LSKAPPVPE 
Sbjct: 121 FMPTPPNGALGSLDYKKEERRELAIRSREGSPKFGTPDRQKVIDEIHAYMLSKAPPVPEI 180

Query: 181 LKQTSEEHQSEKEAEEAEGSENAGRSADSVEPENEGGERITEEPQAAVENADAVEQREAI 240
           LKQ  +E  +E+EA EAEGSENAGRSA +VE  N  GE I EEPQAAV NA+ V+ REA 
Sbjct: 181 LKQAPDEQSAEREA-EAEGSENAGRSATAVESSNAEGEIINEEPQAAVANANRVDHREAN 240

Query: 241 PRQVRAGVSNQQLQHRPA---QRSTDDRLLTWAAVGLTVAIVFLLLKKLMKSSGYDAIFL 300
           P  VRAG+ NQQL+HRPA   QRS DDRLLTWAAVGLTVAIVFLLLKKL+KS GY AIFL
Sbjct: 241 PGLVRAGILNQQLEHRPAPRIQRSNDDRLLTWAAVGLTVAIVFLLLKKLVKSGGYGAIFL 300

[Close]

BLAST of Spo06145.1 vs. UniProtKB/TrEMBL
Match: W9RG08_9ROSA (Ubiquitin-conjugating enzyme E2 32 OS=Morus notabilis GN=L484_004205 PE=4 SV=1)

HSP 1 Score: 407.1 bits (1045), Expect = 1.800e-110
Identity = 216/304 (71.05%), Postives = 246/304 (80.92%), Query Frame = 1


		  
Query: 3   ATDKYNLKNPAVKRILMEVKEMQTNPSDDFMSVPLEENIFEWQFAIRGPQDTEFEGGIYH 62
           A DKYNLKNPAVKRIL E+KEMQ+N SDDFMS+PLEENIFEWQFAIRGP+DTEFEGGIYH
Sbjct: 2   AEDKYNLKNPAVKRILQEIKEMQSNSSDDFMSLPLEENIFEWQFAIRGPRDTEFEGGIYH 61

Query: 63  GRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFM 122
           GRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFM
Sbjct: 62  GRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFM 121

Query: 123 PTAPNGALGSLDYKKEERRDLAIRSREASPKFGTPERQKVIDEIHEYVLSKAPPVPESLK 182
           PT+PNGALGSLDYKKEERR LA++SREASPKFGTPERQK+IDE+HEY+LSKAPPVP+   
Sbjct: 122 PTSPNGALGSLDYKKEERRALAVKSREASPKFGTPERQKLIDEVHEYMLSKAPPVPQLCP 181

Query: 183 QTSEEHQSEK--EAEEAEGSENA--GRSADSVEPENEGGERITEEPQAAVENADAVEQRE 242
             + E Q  K  E +   GS+N+   ++A+ + P    GER+ EE Q +  NA+    RE
Sbjct: 182 SETPEEQPTKIEEGDAQAGSQNSDVAQAAEGL-PNPSVGERVVEEHQESPANANPRLARE 241

Query: 243 AIPRQVRAGVSNQQLQHRP--AQRSTDDRLLTWAAVGLTVAIVFLLLKKLMKSSGYDAIF 301
             P +V +  SNQ LQ +   AQR  DDRL TWAAVGLT+AIV LLLKK M + G+ A+F
Sbjct: 242 RAPSEVHS--SNQVLQRQEVRAQRPADDRLFTWAAVGLTIAIVVLLLKKFMNAGGHGAVF 301

[Close]

BLAST of Spo06145.1 vs. UniProtKB/TrEMBL
Match: A0A061ENX3_THECC (Ubiquitin-conjugating enzyme 32 OS=Theobroma cacao GN=TCM_021352 PE=4 SV=1)

HSP 1 Score: 406.4 bits (1043), Expect = 3.100e-110
Identity = 216/304 (71.05%), Postives = 245/304 (80.59%), Query Frame = 1


		  
Query: 3   ATDKYNLKNPAVKRILMEVKEMQTNPSDDFMSVPLEENIFEWQFAIRGPQDTEFEGGIYH 62
           A DKYNLKNPAVKRIL EVKEMQ+NPSDDFMS+PLEENIFEWQFAIRGP+++EFEGGIYH
Sbjct: 2   AEDKYNLKNPAVKRILQEVKEMQSNPSDDFMSLPLEENIFEWQFAIRGPRESEFEGGIYH 61

Query: 63  GRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFM 122
           GRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFM
Sbjct: 62  GRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFM 121

Query: 123 PTAPNGALGSLDYKKEERRDLAIRSREASPKFGTPERQKVIDEIHEYVLSKAPPVPE-SL 182
           PT+PNGALGSLDYKKEERR LAI+SREA PKFG PERQK+IDEIHEY+LSKAPPVP+ S 
Sbjct: 122 PTSPNGALGSLDYKKEERRALAIKSREAPPKFGNPERQKLIDEIHEYMLSKAPPVPQLSP 181

Query: 183 KQTSEEHQSEKE-AEEAEGSENAGRSADSVEPENEGGERITEEPQAAVENADAVEQREAI 242
            Q SEEH  +++   +A   ++   +     P    G+R+ EE   A  NA+       +
Sbjct: 182 SQASEEHSMDRDGVTQANQQDSVSMAGGDGLPNQAVGDRVVEEEPVAPANANPAPAEIRV 241

Query: 243 PRQVRA-GVSNQQLQHRP---AQRSTDDRLLTWAAVGLTVAIVFLLLKKLMKSSGYDAIF 301
            R+V A G SNQ LQ RP    Q+S DDRL TWAAVGLT+AI+ LLLKK +KSSG+ A+F
Sbjct: 242 VREVPARGPSNQPLQ-RPELRVQKSADDRLFTWAAVGLTIAILVLLLKKFLKSSGHGAVF 301

[Close]

BLAST of Spo06145.1 vs. UniProtKB/TrEMBL
Match: B9RZP3_RICCO (Non-canonical ubiquitin conjugating enzyme, putative OS=Ricinus communis GN=RCOM_1000310 PE=4 SV=1)

HSP 1 Score: 402.1 bits (1032), Expect = 5.800e-109
Identity = 215/304 (70.72%), Postives = 242/304 (79.61%), Query Frame = 1


		  
Query: 3   ATDKYNLKNPAVKRILMEVKEMQTNPSDDFMSVPLEENIFEWQFAIRGPQDTEFEGGIYH 62
           A DKYNLKNPAVKRIL EVKEMQ+NPSDDFMS+PLEENIFEWQFAIRGP DTEFEGGIYH
Sbjct: 2   AEDKYNLKNPAVKRILQEVKEMQSNPSDDFMSMPLEENIFEWQFAIRGPCDTEFEGGIYH 61

Query: 63  GRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFM 122
           GRIQLP+EYPFKPPSF+LLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFM
Sbjct: 62  GRIQLPSEYPFKPPSFLLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFM 121

Query: 123 PTAPNGALGSLDYKKEERRDLAIRSREASPKFGTPERQKVIDEIHEYVLSKAPPVPE-SL 182
           PT PNGALGSLDYKKEERR LAI+SREA+P+FGTPERQK+IDEIH+Y+LSKAPPVP+ S 
Sbjct: 122 PTNPNGALGSLDYKKEERRALAIKSREAAPRFGTPERQKLIDEIHQYMLSKAPPVPQLSP 181

Query: 183 KQTSEEHQSEKEAE-----EAEGSENAGRSADSVEPENEGGERITEEPQAAVENADAVEQ 242
            Q SEEH + KE E     + +G+  A     +V  +   G+RI EE   A  N +    
Sbjct: 182 SQDSEEHHTGKEDEAHAILQEQGAVAANLPNLAVSEQLAVGDRIVEEVPEAPVNVNPGAT 241

Query: 243 REAIPRQVRAGVSNQQLQHRP---AQRSTDDRLLTWAAVGLTVAIVFLLLKKLMKSSGYD 298
                 ++ A  S+ QL HRP    QR  DDRL TWAAVGLT+AIV LLLKK +KSSG+ 
Sbjct: 242 GVRTSTEIPARASSDQLLHRPETRVQRPADDRLFTWAAVGLTIAIVVLLLKKFLKSSGHG 301

[Close]

BLAST of Spo06145.1 vs. ExPASy Swiss-Prot
Match: UBC32_ARATH (Ubiquitin-conjugating enzyme E2 32 OS=Arabidopsis thaliana GN=UBC32 PE=2 SV=1)

HSP 1 Score: 362.5 bits (929), Expect = 4.600e-99
Identity = 193/306 (63.07%), Postives = 224/306 (73.20%), Query Frame = 1


		  
Query: 3   ATDKYNLKNPAVKRILMEVKEMQTNPSDDFMSVPLEENIFEWQFAIRGPQDTEFEGGIYH 62
           A ++YN KNPAVKRIL EVKEMQ NPSDDFMS+PLEENIFEWQFAIRGP DTEFEGGIYH
Sbjct: 2   ADERYNRKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDTEFEGGIYH 61

Query: 63  GRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFM 122
           GRIQLPA+YPFKPPSFMLLTPNGRFET TKICLSISN+HPEHWQPSWSVRTALVALIAFM
Sbjct: 62  GRIQLPADYPFKPPSFMLLTPNGRFETNTKICLSISNYHPEHWQPSWSVRTALVALIAFM 121

Query: 123 PTAPNGALGSLDYKKEERRDLAIRSREASPKFGTPERQKVIDEIHEYVLSKAPPVPESL- 182
           PT+PNGALGS+DY K+ERR LAI+SRE  PK+G+PERQK+IDEIH+Y+LSKA  VP+ L 
Sbjct: 122 PTSPNGALGSVDYPKDERRTLAIKSRETPPKYGSPERQKIIDEIHQYILSKATVVPKPLP 181

Query: 183 -------KQTSEEHQSEKEAEEAEGSENAGRSADSVEPENEGGERITEEPQAAVENADAV 242
                     SE H   +  E     E    +      +++  E   E    A     A 
Sbjct: 182 LECSQAPSIVSEAHSQVEPQEAITVVEERSIATTDTIVDDQIIEETAEAVNTAASVVPAA 241

Query: 243 EQREAIPRQVRAGVSNQQ-LQHRPAQRSTDDRLLTWAAVGLTVAIVFLLLKKLMKSSGYD 300
               A+   V+A VS +Q +  R AQ+  DDRL TWAAVGLT+AI+ LLLKK +KS+GY 
Sbjct: 242 APLPAVEVVVKASVSGEQRMARRAAQKPVDDRLFTWAAVGLTIAIMVLLLKKFIKSNGYS 301

[Close]

BLAST of Spo06145.1 vs. ExPASy Swiss-Prot
Match: UB2J1_MOUSE (Ubiquitin-conjugating enzyme E2 J1 OS=Mus musculus GN=Ube2j1 PE=1 SV=2)

HSP 1 Score: 204.9 bits (520), Expect = 1.200e-51
Identity = 91/144 (63.19%), Postives = 116/144 (80.56%), Query Frame = 1


		  
Query: 6   KYNLKNPAVKRILMEVKEMQTNPSDDFMSVPLEENIFEWQFAIRGPQDTEFEGGIYHGRI 65
           +YNLK+PAVKR++ E  E++ +P+D + + PLE+N+FEW F +RGP D++F+GG+YHGRI
Sbjct: 4   RYNLKSPAVKRLMKEAAELK-DPTDHYHAQPLEDNLFEWHFTVRGPPDSDFDGGVYHGRI 63

Query: 66  QLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFMPTA 125
            LP EYP KPPS +LLT NGRFE   KICLSIS HHPE WQPSWS+RTAL+A+I FMPT 
Sbjct: 64  VLPPEYPMKPPSIILLTANGRFEVGKKICLSISGHHPETWQPSWSIRTALLAIIGFMPTK 123

Query: 126 PNGALGSLDYKKEERRDLAIRSRE 150
             GA+GSLDY  EERR LA +S++
Sbjct: 124 GEGAIGSLDYTPEERRALAKKSQD 146

[Close]

BLAST of Spo06145.1 vs. ExPASy Swiss-Prot
Match: UB2J1_HUMAN (Ubiquitin-conjugating enzyme E2 J1 OS=Homo sapiens GN=UBE2J1 PE=1 SV=2)

HSP 1 Score: 199.9 bits (507), Expect = 3.900e-50
Identity = 102/191 (53.40%), Postives = 131/191 (68.59%), Query Frame = 1


		  
Query: 6   KYNLKNPAVKRILMEVKEMQTNPSDDFMSVPLEENIFEWQFAIRGPQDTEFEGGIYHGRI 65
           +YNLK+PAVKR++ E  E++ +P+D + + PLE+N+FEW F +RGP D++F+GG+YHGRI
Sbjct: 4   RYNLKSPAVKRLMKEAAELK-DPTDHYHAQPLEDNLFEWHFTVRGPPDSDFDGGVYHGRI 63

Query: 66  QLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFMPTA 125
            LP EYP KPPS +LLT NGRFE   KICLSIS HHPE WQPSWS+RTAL+A+I FMPT 
Sbjct: 64  VLPPEYPMKPPSIILLTANGRFEVGKKICLSISGHHPETWQPSWSIRTALLAIIGFMPTK 123

Query: 126 PNGALGSLDYKKEERRDLAIRSREASPKFGTPERQKVIDEIHEYVLSKAPPVPESLKQTS 185
             GA+GSLDY  EERR LA +S+               D   E   S    V   LK  S
Sbjct: 124 GEGAIGSLDYTPEERRALAKKSQ---------------DFCCEGCGSAMKDVLLPLKSGS 178

Query: 186 EEHQSEKEAEE 197
           +  Q+++EA+E
Sbjct: 184 DSSQADQEAKE 178

[Close]

BLAST of Spo06145.1 vs. ExPASy Swiss-Prot
Match: UB2J2_HUMAN (Ubiquitin-conjugating enzyme E2 J2 OS=Homo sapiens GN=UBE2J2 PE=1 SV=3)

HSP 1 Score: 137.1 bits (344), Expect = 3.100e-31
Identity = 59/147 (40.14%), Postives = 91/147 (61.90%), Query Frame = 1


		  
Query: 1   MAATDKYNLKNPAVKRILMEVKEMQTNPSDDFMSVPLEENIFEWQFAIRGPQDTEFEGGI 60
           M++T        A +R+  +   ++ +P     + PL  NI EW + +RGP+ T +EGG 
Sbjct: 1   MSSTSSKRAPTTATQRLKQDYLRIKKDPVPYICAEPLPSNILEWHYVVRGPEMTPYEGGY 60

Query: 61  YHGRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIA 120
           YHG++  P E+PFKPPS  ++TPNGRF+  T++CLSI++ HP+ W P+WSV T L  L++
Sbjct: 61  YHGKLIFPREFPFKPPSIYMITPNGRFKCNTRLCLSITDFHPDTWNPAWSVSTILTGLLS 120

Query: 121 FMPTAPNGALGSLDYKKEERRDLAIRS 148
           FM       LGS++     +R LA++S
Sbjct: 121 FM-VEKGPTLGSIETSDFTKRQLAVQS 146

[Close]

BLAST of Spo06145.1 vs. ExPASy Swiss-Prot
Match: UBC6_ASHGO (Ubiquitin-conjugating enzyme E2 6 OS=Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) GN=UBC6 PE=3 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 5.300e-31
Identity = 59/137 (43.07%), Postives = 86/137 (62.77%), Query Frame = 1


		  
Query: 13  AVKRILMEVKEMQTNPSDDFMSVPLEENIFEWQFAIRGPQDTEFEGGIYHGRIQLPAEYP 72
           A KR+  E K M  NP    ++ P E+NI  W + I GP +T +E G YHG +  P +YP
Sbjct: 6   AYKRLSKEYKMMTENPPPYIVAAPKEDNILVWHYVITGPPETPYEDGQYHGTLVFPNDYP 65

Query: 73  FKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFMPTAPNGALGS 132
           F PP+  +LTPNGRF   T++CLS+S++HP+ W PSWSV T L  L++FM T    ++G+
Sbjct: 66  FNPPAIRMLTPNGRFRENTRLCLSMSDYHPDTWNPSWSVATILTGLLSFM-TTDESSIGT 125

Query: 133 LDYKKEERRDLAIRSRE 150
           ++     +R  A +S+E
Sbjct: 126 MESTDSTKRKYAAKSKE 141

[Close]

BLAST of Spo06145.1 vs. TAIR (Arabidopsis)
Match: AT3G17000.1 (ubiquitin-conjugating enzyme 32)

HSP 1 Score: 362.5 bits (929), Expect = 2.600e-100
Identity = 193/306 (63.07%), Postives = 224/306 (73.20%), Query Frame = 1


		  
Query: 3   ATDKYNLKNPAVKRILMEVKEMQTNPSDDFMSVPLEENIFEWQFAIRGPQDTEFEGGIYH 62
           A ++YN KNPAVKRIL EVKEMQ NPSDDFMS+PLEENIFEWQFAIRGP DTEFEGGIYH
Sbjct: 2   ADERYNRKNPAVKRILQEVKEMQANPSDDFMSLPLEENIFEWQFAIRGPGDTEFEGGIYH 61

Query: 63  GRIQLPAEYPFKPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFM 122
           GRIQLPA+YPFKPPSFMLLTPNGRFET TKICLSISN+HPEHWQPSWSVRTALVALIAFM
Sbjct: 62  GRIQLPADYPFKPPSFMLLTPNGRFETNTKICLSISNYHPEHWQPSWSVRTALVALIAFM 121

Query: 123 PTAPNGALGSLDYKKEERRDLAIRSREASPKFGTPERQKVIDEIHEYVLSKAPPVPESL- 182
           PT+PNGALGS+DY K+ERR LAI+SRE  PK+G+PERQK+IDEIH+Y+LSKA  VP+ L 
Sbjct: 122 PTSPNGALGSVDYPKDERRTLAIKSRETPPKYGSPERQKIIDEIHQYILSKATVVPKPLP 181

Query: 183 -------KQTSEEHQSEKEAEEAEGSENAGRSADSVEPENEGGERITEEPQAAVENADAV 242
                     SE H   +  E     E    +      +++  E   E    A     A 
Sbjct: 182 LECSQAPSIVSEAHSQVEPQEAITVVEERSIATTDTIVDDQIIEETAEAVNTAASVVPAA 241

Query: 243 EQREAIPRQVRAGVSNQQ-LQHRPAQRSTDDRLLTWAAVGLTVAIVFLLLKKLMKSSGYD 300
               A+   V+A VS +Q +  R AQ+  DDRL TWAAVGLT+AI+ LLLKK +KS+GY 
Sbjct: 242 APLPAVEVVVKASVSGEQRMARRAAQKPVDDRLFTWAAVGLTIAIMVLLLKKFIKSNGYS 301

[Close]

BLAST of Spo06145.1 vs. TAIR (Arabidopsis)
Match: AT1G17280.1 (ubiquitin-conjugating enzyme 34)

HSP 1 Score: 120.2 bits (300), Expect = 2.200e-27
Identity = 70/200 (35.00%), Postives = 112/200 (56.00%), Query Frame = 1


		  
Query: 14  VKRILMEVKEMQTNPSDDFMSVPLEENIFEWQFAIRGPQDTEFEGGIYHGRIQLPAEYPF 73
           +KR+  E + +   P    ++ P   +I EW + + G + T F GG Y+G+I+ P EYP+
Sbjct: 7   IKRLQKEYRALCKEPVSHVVARPSPNDILEWHYVLEGSEGTPFAGGFYYGKIKFPPEYPY 66

Query: 74  KPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAF-MPTAPNGALGS 133
           KPP   + TPNGRF TQ KICLS+S+ HPE W P WSV + L  L++F M T+P    GS
Sbjct: 67  KPPGITMTTPNGRFMTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFMMDTSP--TTGS 126

Query: 134 LDYKKEERRDLAIRSREASPKFGTPERQKV----IDEIHEYVLSKAP----PVPESLKQT 193
           ++    E++ LA  S   + K  TP  +K+    +++ ++  L++        PES +++
Sbjct: 127 VNTTVIEKQRLAKSSLAFNCK--TPAFRKLFPEYVEKYNQQQLAEQATTQLTTPESPQKS 186

Query: 194 SEEHQSEKEAEEAEGSENAG 205
             + +SEK  +  +G    G
Sbjct: 187 DTKVESEKTIDPTKGDSEGG 202

[Close]

BLAST of Spo06145.1 vs. TAIR (Arabidopsis)
Match: AT5G50430.1 (ubiquitin-conjugating enzyme 33)

HSP 1 Score: 115.5 bits (288), Expect = 5.500e-26
Identity = 67/201 (33.33%), Postives = 110/201 (54.73%), Query Frame = 1


		  
Query: 14  VKRILMEVKEMQTNPSDDFMSVPLEENIFEWQFAIRGPQDTEFEGGIYHGRIQLPAEYPF 73
           +KR+  E + +   P    ++ P   +I EW + + G + T F GG Y+G+I+ P EYP+
Sbjct: 7   IKRLQKEYRALCKEPVSHVVARPSPNDILEWHYVLEGSEGTPFAGGFYYGKIKFPPEYPY 66

Query: 74  KPPSFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFMPTAPNGALGSL 133
           KPP   + TPNGRF TQ KICLS+S+ HPE W P WSV + L  L++FM    +   GS+
Sbjct: 67  KPPGITMTTPNGRFVTQKKICLSMSDFHPESWNPMWSVSSILTGLLSFM-MDNSPTTGSV 126

Query: 134 DYKKEERRDLAIRSREASPKFGTPERQKVIDEIHEYVLSKAPPVPESLKQ-TSEEHQS-- 193
           +    E++ LA  S   + K  T  R+   + + +Y   +      + +Q T+ E+Q   
Sbjct: 127 NTSVAEKQRLAKSSLAFNCKSVT-FRKLFPEYVEKYSQQQVAEEEAATQQTTTSENQDFP 186

Query: 194 EKEAEEAEGSENAGRSADSVE 212
           +K+  + E  ++ G   +S++
Sbjct: 187 QKDNAKVESEKSVGLKKESIQ 205

[Close]

BLAST of Spo06145.1 vs. TAIR (Arabidopsis)
Match: AT4G27960.2 (ubiquitin conjugating enzyme 9)

HSP 1 Score: 80.1 bits (196), Expect = 2.600e-15
Identity = 40/117 (34.19%), Postives = 63/117 (53.85%), Query Frame = 1


		  
Query: 13  AVKRILMEVKEMQTNPSDDFMSVPLEENIFEWQFAIRGPQDTEFEGGIYHGRIQLPAEYP 72
           A KRIL E+K++Q +P     + P+ E++F WQ  I GP D+ + GG++   I  P +YP
Sbjct: 32  ASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPSDSPYSGGVFLVTIHFPPDYP 91

Query: 73  FKPPSFMLLTP--NGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFMPTAPN 128
           FKPP     T   +    +   ICL I     E W P+ ++   L+++ + + T PN
Sbjct: 92  FKPPKVAFRTKVFHPNINSNGSICLDILK---EQWSPALTISKVLLSICSLL-TDPN 144

[Close]

BLAST of Spo06145.1 vs. TAIR (Arabidopsis)
Match: AT3G08690.1 (ubiquitin-conjugating enzyme 11)

HSP 1 Score: 79.7 bits (195), Expect = 3.300e-15
Identity = 40/117 (34.19%), Postives = 65/117 (55.56%), Query Frame = 1


		  
Query: 13  AVKRILMEVKEMQTNPSDDFMSVPLEENIFEWQFAIRGPQDTEFEGGIYHGRIQLPAEYP 72
           A KRIL E+K++Q +P  +  + P+ E++F WQ  I GP ++ + GG++   I  P +YP
Sbjct: 2   ASKRILKELKDLQKDPPSNCSAGPVAEDMFHWQATIMGPPESPYAGGVFLVSIHFPPDYP 61

Query: 73  FKPP--SFMLLTPNGRFETQTKICLSISNHHPEHWQPSWSVRTALVALIAFMPTAPN 128
           FKPP  SF     +    +   ICL I     E W P+ ++   L+++ + + T PN
Sbjct: 62  FKPPKVSFKTKVYHPNINSNGSICLDILK---EQWSPALTISKVLLSICSLL-TDPN 114

The following BLAST results are available for this feature:

BLAST of Spo06145.1 vs. NCBI nr
BLAST of Spo06145.1 vs. UniProtKB/TrEMBL
BLAST of Spo06145.1 vs. ExPASy Swiss-Prot
BLAST of Spo06145.1 vs. TAIR (Arabidopsis)

BLAST of Spo06145.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902179648\|gb\|KNA09393.1\|	4.1e-164	100.	hypothetical protein SOVF_1538...	[more]
gi\|731322080\|ref\|XP_010671695.1\|	8.0e-136	84.4	PREDICTED: ubiquitin-conjugati...	[more]
gi\|703103678\|ref\|XP_010097791.1\|	2.6e-110	71.0	Ubiquitin-conjugating enzyme E...	[more]
gi\|590661821\|ref\|XP_007035779.1\|	4.4e-110	71.0	Ubiquitin-conjugating enzyme 3...	[more]
gi\|255556356\|ref\|XP_002519212.1\|	8.3e-109	70.7	PREDICTED: ubiquitin-conjugati...	[more]

BLAST of Spo06145.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9QRS7_SPIOL	2.8e-164	100.	Uncharacterized protein OS=Spi...	[more]
A0A0J8CR27_BETVU	5.6e-136	84.4	Uncharacterized protein OS=Bet...	[more]
W9RG08_9ROSA	1.8e-110	71.0	Ubiquitin-conjugating enzyme E...	[more]
A0A061ENX3_THECC	3.1e-110	71.0	Ubiquitin-conjugating enzyme 3...	[more]
B9RZP3_RICCO	5.8e-109	70.7	Non-canonical ubiquitin conjug...	[more]

BLAST of Spo06145.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
UBC32_ARATH	4.6e-99	63.0	Ubiquitin-conjugating enzyme E...	[more]
UB2J1_MOUSE	1.2e-51	63.1	Ubiquitin-conjugating enzyme E...	[more]
UB2J1_HUMAN	3.9e-50	53.4	Ubiquitin-conjugating enzyme E...	[more]
UB2J2_HUMAN	3.1e-31	40.1	Ubiquitin-conjugating enzyme E...	[more]
UBC6_ASHGO	5.3e-31	43.0	Ubiquitin-conjugating enzyme E...	[more]

BLAST of Spo06145.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 5


Match Name	E-value	Identity	Description
AT3G17000.1	2.6e-100	63.0	ubiquitin-conjugating enzyme 3...	[more]
AT1G17280.1	2.2e-27	35.0	ubiquitin-conjugating enzyme 3...	[more]
AT5G50430.1	5.5e-26	33.3	ubiquitin-conjugating enzyme 3...	[more]
AT4G27960.2	2.6e-15	34.1	ubiquitin conjugating enzyme 9	[more]
AT3G08690.1	3.3e-15	34.1	ubiquitin-conjugating enzyme 1...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000608	Ubiquitin-conjugating enzyme E2	PFAM	PF00179	UQ_con	coord: 16..127 score: 1.2
IPR000608	Ubiquitin-conjugating enzyme E2	PROFILE	PS50127	UBIQUITIN_CONJUGAT_2	coord: 15..127 score: 23
IPR016135	Ubiquitin-conjugating enzyme/RWD-like	GENE3D	3.10.110.10		coord: 8..211 score: 1.4
IPR016135	Ubiquitin-conjugating enzyme/RWD-like	unknown	SSF54495	UBC-like	coord: 13..135 score: 2.42
None	No IPR available	PANTHER	PTHR24067	UBIQUITIN-CONJUGATING ENZYME E2	coord: 1..149 score: 6.9
None	No IPR available	PANTHER	PTHR24067:SF54	UBIQUITIN-CONJUGATING ENZYME E2 J1	coord: 1..149 score: 6.9
None	No IPR available	SMART	SM00212	ubc_7	coord: 15..175 score: 6.2

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009056	catabolic process
biological_process	GO:0044249	cellular biosynthetic process
biological_process	GO:0044255	cellular lipid metabolic process
biological_process	GO:0031668	cellular response to extracellular stimulus
biological_process	GO:0033554	cellular response to stress
biological_process	GO:0090304	nucleic acid metabolic process
biological_process	GO:1901576	organic substance biosynthetic process
biological_process	GO:0042221	response to chemical
biological_process	GO:0006511	ubiquitin-dependent protein catabolic process
cellular_component	GO:0044444	cytoplasmic part
cellular_component	GO:0016020	membrane
molecular_function	GO:0016874	ligase activity
molecular_function	GO:0000166	nucleotide binding