Spo07563.1 (mRNA) | SpinachBase

Overview


Name	Spo07563.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	Subtilisin-like serine protease
Location	SpoScf_07174 : 1916 .. 3066 (-)
Sequence length	608

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TAAGTAAAAATAAAAATCCAAAATCTTGAAAAGATAACCCCCAATATTCTATGAGATTCTACTAGTACAAATACCACTCATAGTTGTACATCTTCTTTTATCCCAAAAACAAAACCAAAGTGATAAAAAAAAAACCTTGATCAATGGAGATAATCTCAAAAATCTTATTTCTCTTCTTTTTCCTCCTCCCAATTCTAGCTGCCTCCTCGGAAAATCAGGTATGCAATTAGTGTGCTTAATACGAATACGAATACGAATACGAATACGAATGAAGTAGTATGTTAATTACAATTTACAAATTTATGGTAACGTTATAATTATGTATTGTTTGTGTATGATAAGATGTTATATATATATATATAATGGCAGGTTTATATAGTATACTTGGGAGGGCATAAGGGAGATAAAGAAGTACATGAGATAGAACAATTTCATCACTCTTACTTAGCCTCTGTTAAGGAAACAGAGCAAGAGGCTAGAGCATCTATGCTTTACAGTTACAAACATAGTATTAATGGCTTTGCTGCTACTCTTACTCCCGATCAAGCCTCTAAACTATCTGGTATTCCATTCGTCTTCCCTTCAATTCTTCCGCTTTTCTTTACTCCTTTTTTACTTGAGATTTAAAACATTTCCTCTTTATTAACAGTCACAGTCACAGTCACGTTGATTGTGTAATTTGAATCGGTAACATGTCACTTAAATCTAAAAATCAATGTTTATGCTAATGGCTTTTAATGATTTATACCATTGTTACATTGAATTTTACTGATATCAAATTATACCATGTTGATATTTGACTATGGCAAAAGGTTAAAGGTTTGTTAAAATTTTGCCCTAAGCCTAAAGAGTAAAGACCAAGTGCCCCCGAGCCAAATCTACTTTTATATTTAATTTAATTTAATTTAATTTAATTTAATTTAATGAAATGAATTTGTATTGTAATTTGAATAGAATTGGAGGAAGTGATATCGGTGTTTCGAAGCCATCCAATGAAGCACGAAATGCATACAACAAGGTCATGGGAATTCGTAGGGTTGGAGGAAGGAATCAAAACAACACAGATGCACTCTCATACAGCGGGGATGACTCAAGGTTTACTGTCAAAAGCAAAGTATGGACACAATGTCATCGTCGGGGTCTTAGATAGT

mRNA sequence

TAAGTAAAAATAAAAATCCAAAATCTTGAAAAGATAACCCCCAATATTCTATGAGATTCTACTAGTACAAATACCACTCATAGTTGTACATCTTCTTTTATCCCAAAAACAAAACCAAAGTGATAAAAAAAAAACCTTGATCAATGGAGATAATCTCAAAAATCTTATTTCTCTTCTTTTTCCTCCTCCCAATTCTAGCTGCCTCCTCGGAAAATCAGGTTTATATAGTATACTTGGGAGGGCATAAGGGAGATAAAGAAGTACATGAGATAGAACAATTTCATCACTCTTACTTAGCCTCTGTTAAGGAAACAGAGCAAGAGGCTAGAGCATCTATGCTTTACAGTTACAAACATAGTATTAATGGCTTTGCTGCTACTCTTACTCCCGATCAAGCCTCTAAACTATCTGAATTGGAGGAAGTGATATCGGTGTTTCGAAGCCATCCAATGAAGCACGAAATGCATACAACAAGGTCATGGGAATTCGTAGGGTTGGAGGAAGGAATCAAAACAACACAGATGCACTCTCATACAGCGGGGATGACTCAAGGTTTACTGTCAAAAGCAAAGTATGGACACAATGTCATCGTCGGGGTCTTAGATAGT

Coding sequence (CDS)

ATGGAGATAATCTCAAAAATCTTATTTCTCTTCTTTTTCCTCCTCCCAATTCTAGCTGCCTCCTCGGAAAATCAGGTTTATATAGTATACTTGGGAGGGCATAAGGGAGATAAAGAAGTACATGAGATAGAACAATTTCATCACTCTTACTTAGCCTCTGTTAAGGAAACAGAGCAAGAGGCTAGAGCATCTATGCTTTACAGTTACAAACATAGTATTAATGGCTTTGCTGCTACTCTTACTCCCGATCAAGCCTCTAAACTATCTGAATTGGAGGAAGTGATATCGGTGTTTCGAAGCCATCCAATGAAGCACGAAATGCATACAACAAGGTCATGGGAATTCGTAGGGTTGGAGGAAGGAATCAAAACAACACAGATGCACTCTCATACAGCGGGGATGACTCAAGGTTTACTGTCAAAAGCAAAGTATGGACACAATGTCATCGTCGGGGTCTTAGATAGT

Protein sequence

MEIISKILFLFFFLLPILAASSENQVYIVYLGGHKGDKEVHEIEQFHHSYLASVKETEQEARASMLYSYKHSINGFAATLTPDQASKLSELEEVISVFRSHPMKHEMHTTRSWEFVGLEEGIKTTQMHSHTAGMTQGLLSKAKYGHNVIVGVLDS

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo07563	Spo07563	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo07563.1	Spo07563.1-protein	polypeptide

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo07563.1.utr5p.1	Spo07563.1.utr5p.1	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo07563.1.CDS.3	Spo07563.1.CDS.3	CDS
Spo07563.1.CDS.2	Spo07563.1.CDS.2	CDS
Spo07563.1.CDS.1	Spo07563.1.CDS.1	CDS

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo07563.1.exon.3	Spo07563.1.exon.3	exon
Spo07563.1.exon.2	Spo07563.1.exon.2	exon
Spo07563.1.exon.1	Spo07563.1.exon.1	exon

Homology

[Close]

BLAST of Spo07563.1 vs. NCBI nr
Match: gi|902181395|gb|KNA09728.1| (hypothetical protein SOVF_150930 [Spinacia oleracea])

HSP 1 Score: 304.7 bits (779), Expect = 9.400e-80
Identity = 155/155 (100.00%), Postives = 155/155 (100.00%), Query Frame = 1


		  
Query: 1   MEIISKILFLFFFLLPILAASSENQVYIVYLGGHKGDKEVHEIEQFHHSYLASVKETEQE 60
           MEIISKILFLFFFLLPILAASSENQVYIVYLGGHKGDKEVHEIEQFHHSYLASVKETEQE
Sbjct: 1   MEIISKILFLFFFLLPILAASSENQVYIVYLGGHKGDKEVHEIEQFHHSYLASVKETEQE 60

Query: 61  ARASMLYSYKHSINGFAATLTPDQASKLSELEEVISVFRSHPMKHEMHTTRSWEFVGLEE 120
           ARASMLYSYKHSINGFAATLTPDQASKLSELEEVISVFRSHPMKHEMHTTRSWEFVGLEE
Sbjct: 61  ARASMLYSYKHSINGFAATLTPDQASKLSELEEVISVFRSHPMKHEMHTTRSWEFVGLEE 120

Query: 121 GIKTTQMHSHTAGMTQGLLSKAKYGHNVIVGVLDS 156
           GIKTTQMHSHTAGMTQGLLSKAKYGHNVIVGVLDS
Sbjct: 121 GIKTTQMHSHTAGMTQGLLSKAKYGHNVIVGVLDS 155

[Close]

BLAST of Spo07563.1 vs. NCBI nr
Match: gi|731370210|ref|XP_010665649.1| (PREDICTED: subtilisin-like protease SBT5.3 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 231.5 bits (589), Expect = 1.000e-57
Identity = 119/155 (76.77%), Postives = 136/155 (87.74%), Query Frame = 1


		  
Query: 1   MEIISKILFLFFFLLPILAASSENQVYIVYLGGHKGDKEVHEIEQFHHSYLASVKETEQE 60
           MEI+S I  LF F+LPILA+SS++QVYIVY G HKGDK++HEIE++HHSYLASVKETE+E
Sbjct: 1   MEILSTIFSLFLFVLPILASSSQSQVYIVYFGEHKGDKKLHEIEEYHHSYLASVKETEEE 60

Query: 61  ARASMLYSYKHSINGFAATLTPDQASKLSELEEVISVFRSHPMKHEMHTTRSWEFVGLEE 120
           AR SMLYSYKHSINGFAATLTP QASKLSELEEV+SVF SHP KH+MHTTRSWEFVGLEE
Sbjct: 61  ARVSMLYSYKHSINGFAATLTPTQASKLSELEEVVSVFPSHPRKHKMHTTRSWEFVGLEE 120

Query: 121 GIKTTQMHSHTAGMTQGLLSKAKYGHNVIVGVLDS 156
           GIK  Q+H H    ++ LL++AKYG NVIVGVLDS
Sbjct: 121 GIK-QQLHMH-MDKSEALLTRAKYGRNVIVGVLDS 153

[Close]

BLAST of Spo07563.1 vs. NCBI nr
Match: gi|743932273|ref|XP_011010424.1| (PREDICTED: subtilisin-like protease SBT5.3 [Populus euphratica])

HSP 1 Score: 184.5 bits (467), Expect = 1.400e-43
Identity = 95/154 (61.69%), Postives = 118/154 (76.62%), Query Frame = 1


		  
Query: 4   ISKILFLFFFLLPILAASSENQVYIVYLGGHKGDKEVHEIEQFHHSYLASVKETEQEARA 63
           + K+  LF FLLP+LA+  E QVYIVY G HKGDK +HEIE+FHHSYL  VK+TE+EA+A
Sbjct: 1   MKKLSILFLFLLPLLASCEEKQVYIVYFGEHKGDKALHEIEEFHHSYLYGVKQTEEEAKA 60

Query: 64  SMLYSYKHSINGFAATLTPDQASKLSELEEVISVFRSHPMKHEMHTTRSWEFVGLEEGIK 123
           S+LYSYKHSINGFAA L PD+ASKLSEL+EV+SVF+S+P K+ + TTRSW F GLEE   
Sbjct: 61  SLLYSYKHSINGFAALLNPDEASKLSELKEVVSVFKSNPRKYSVQTTRSWRFAGLEE--- 120

Query: 124 TTQMHSHTAGMTQG--LLSKAKYGHNVIVGVLDS 156
             + H+   G  +G  LL +A YG  VIVG++DS
Sbjct: 121 --EGHNVNHGFGEGSDLLKRAGYGKQVIVGLIDS 149

[Close]

BLAST of Spo07563.1 vs. NCBI nr
Match: gi|970010684|ref|XP_015066240.1| (PREDICTED: subtilisin-like protease SBT5.3 [Solanum pennellii])

HSP 1 Score: 183.3 bits (464), Expect = 3.100e-43
Identity = 91/155 (58.71%), Postives = 116/155 (74.84%), Query Frame = 1


		  
Query: 1   MEIISKILFLFFFLLPILAASSENQVYIVYLGGHKGDKEVHEIEQFHHSYLASVKETEQE 60
           M   S + FL   +LP+LA+  E QVYIVY GGH G+K +HEIE+ HHSYL SVK+ E+E
Sbjct: 1   MNSFSTLSFLLLLVLPLLASCHEKQVYIVYFGGHNGEKALHEIEENHHSYLLSVKDNEEE 60

Query: 61  ARASMLYSYKHSINGFAATLTPDQASKLSELEEVISVFRSHPMKHEMHTTRSWEFVGLEE 120
           A++S++YSYKHSINGFAA LTP QASKLSELEEV+SV++S P K+ +HTTRSWEF G+EE
Sbjct: 61  AKSSLIYSYKHSINGFAALLTPHQASKLSELEEVVSVYKSEPRKYSLHTTRSWEFSGVEE 120

Query: 121 GIKTTQMHSHTAGMTQGLLSKAKYGHNVIVGVLDS 156
            +    ++         LL KA+YG N+I+GVLDS
Sbjct: 121 SVAPNYLNK------DDLLLKARYGKNIIIGVLDS 149

[Close]

BLAST of Spo07563.1 vs. NCBI nr
Match: gi|743903976|ref|XP_011045349.1| (PREDICTED: subtilisin-like protease SBT5.3 isoform X2 [Populus euphratica])

HSP 1 Score: 183.0 bits (463), Expect = 4.100e-43
Identity = 94/154 (61.04%), Postives = 118/154 (76.62%), Query Frame = 1


		  
Query: 4   ISKILFLFFFLLPILAASSENQVYIVYLGGHKGDKEVHEIEQFHHSYLASVKETEQEARA 63
           + K+  LF FLLP+LA+  E QVYIVY G HKG+K +HEIE+FHHSYL  VK+TE+EA+A
Sbjct: 1   MKKLSILFLFLLPLLASCEEKQVYIVYFGEHKGEKALHEIEEFHHSYLYGVKQTEEEAKA 60

Query: 64  SMLYSYKHSINGFAATLTPDQASKLSELEEVISVFRSHPMKHEMHTTRSWEFVGLEEGIK 123
           S+LYSYKHSINGFAA L PD+ASKLSEL+EV+SVF+S+P K+ + TTRSW F GLEE   
Sbjct: 61  SLLYSYKHSINGFAALLNPDEASKLSELKEVVSVFKSNPRKYSVQTTRSWRFAGLEE--- 120

Query: 124 TTQMHSHTAGMTQG--LLSKAKYGHNVIVGVLDS 156
             + H+   G  +G  LL +A YG  VIVG++DS
Sbjct: 121 --EGHNVNHGFGEGSDLLKRAGYGKQVIVGLIDS 149

[Close]

BLAST of Spo07563.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QSR9_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_150930 PE=4 SV=1)

HSP 1 Score: 304.7 bits (779), Expect = 6.500e-80
Identity = 155/155 (100.00%), Postives = 155/155 (100.00%), Query Frame = 1


		  
Query: 1   MEIISKILFLFFFLLPILAASSENQVYIVYLGGHKGDKEVHEIEQFHHSYLASVKETEQE 60
           MEIISKILFLFFFLLPILAASSENQVYIVYLGGHKGDKEVHEIEQFHHSYLASVKETEQE
Sbjct: 1   MEIISKILFLFFFLLPILAASSENQVYIVYLGGHKGDKEVHEIEQFHHSYLASVKETEQE 60

Query: 61  ARASMLYSYKHSINGFAATLTPDQASKLSELEEVISVFRSHPMKHEMHTTRSWEFVGLEE 120
           ARASMLYSYKHSINGFAATLTPDQASKLSELEEVISVFRSHPMKHEMHTTRSWEFVGLEE
Sbjct: 61  ARASMLYSYKHSINGFAATLTPDQASKLSELEEVISVFRSHPMKHEMHTTRSWEFVGLEE 120

Query: 121 GIKTTQMHSHTAGMTQGLLSKAKYGHNVIVGVLDS 156
           GIKTTQMHSHTAGMTQGLLSKAKYGHNVIVGVLDS
Sbjct: 121 GIKTTQMHSHTAGMTQGLLSKAKYGHNVIVGVLDS 155

[Close]

BLAST of Spo07563.1 vs. UniProtKB/TrEMBL
Match: A0A0J8B6Q9_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_8g200960 PE=4 SV=1)

HSP 1 Score: 231.5 bits (589), Expect = 7.000e-58
Identity = 119/155 (76.77%), Postives = 136/155 (87.74%), Query Frame = 1


		  
Query: 1   MEIISKILFLFFFLLPILAASSENQVYIVYLGGHKGDKEVHEIEQFHHSYLASVKETEQE 60
           MEI+S I  LF F+LPILA+SS++QVYIVY G HKGDK++HEIE++HHSYLASVKETE+E
Sbjct: 1   MEILSTIFSLFLFVLPILASSSQSQVYIVYFGEHKGDKKLHEIEEYHHSYLASVKETEEE 60

Query: 61  ARASMLYSYKHSINGFAATLTPDQASKLSELEEVISVFRSHPMKHEMHTTRSWEFVGLEE 120
           AR SMLYSYKHSINGFAATLTP QASKLSELEEV+SVF SHP KH+MHTTRSWEFVGLEE
Sbjct: 61  ARVSMLYSYKHSINGFAATLTPTQASKLSELEEVVSVFPSHPRKHKMHTTRSWEFVGLEE 120

Query: 121 GIKTTQMHSHTAGMTQGLLSKAKYGHNVIVGVLDS 156
           GIK  Q+H H    ++ LL++AKYG NVIVGVLDS
Sbjct: 121 GIK-QQLHMH-MDKSEALLTRAKYGRNVIVGVLDS 153

[Close]

BLAST of Spo07563.1 vs. UniProtKB/TrEMBL
Match: K4B8D4_SOLLC (Uncharacterized protein OS=Solanum lycopersicum PE=4 SV=1)

HSP 1 Score: 181.0 bits (458), Expect = 1.100e-42
Identity = 90/155 (58.06%), Postives = 115/155 (74.19%), Query Frame = 1


		  
Query: 1   MEIISKILFLFFFLLPILAASSENQVYIVYLGGHKGDKEVHEIEQFHHSYLASVKETEQE 60
           M   S + FL   +LP+LA+  E QVYIVY GGH G+K +HEIE+ HHSYL SVK+ E+E
Sbjct: 1   MNSFSTLSFLLLLVLPLLASCHEKQVYIVYFGGHNGEKALHEIEENHHSYLLSVKDNEEE 60

Query: 61  ARASMLYSYKHSINGFAATLTPDQASKLSELEEVISVFRSHPMKHEMHTTRSWEFVGLEE 120
           A++S++YSYKHSINGFAA LTP QA KLSELEEV+SV++S P K+ +HTTRSWEF G+EE
Sbjct: 61  AKSSLIYSYKHSINGFAALLTPHQAFKLSELEEVVSVYKSEPRKYSLHTTRSWEFSGVEE 120

Query: 121 GIKTTQMHSHTAGMTQGLLSKAKYGHNVIVGVLDS 156
            +    ++         LL KA+YG N+I+GVLDS
Sbjct: 121 SVAPNYLNK------DDLLLKARYGKNIIIGVLDS 149

[Close]

BLAST of Spo07563.1 vs. UniProtKB/TrEMBL
Match: F6HME6_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_10s0003g02170 PE=4 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 1.400e-42
Identity = 88/148 (59.46%), Postives = 114/148 (77.03%), Query Frame = 1


		  
Query: 8   LFLFFFLLPILAASSENQVYIVYLGGHKGDKEVHEIEQFHHSYLASVKETEQEARASMLY 67
           +F   FLLP+LA+ +E +VYIVY GGH G K +HEIE +HHSYL SVK +E+EAR S+LY
Sbjct: 5   VFFLLFLLPLLASCAERKVYIVYFGGHSGQKALHEIEDYHHSYLLSVKASEEEARDSLLY 64

Query: 68  SYKHSINGFAATLTPDQASKLSELEEVISVFRSHPMKHEMHTTRSWEFVGLEEGIKTTQM 127
           SYKHSINGFAA L+P +A+KLSE++EV+SVF S   KH +HTTRSWEFVGLE+G+   Q+
Sbjct: 65  SYKHSINGFAAVLSPQEATKLSEMDEVVSVFPSQRKKHTLHTTRSWEFVGLEKGLGREQL 124

Query: 128 HSHTAGMTQGLLSKAKYGHNVIVGVLDS 156
                  T+ LL KA+YG  +IVG++D+
Sbjct: 125 KKQK--KTRNLLEKARYGDQIIVGMVDN 150

[Close]

BLAST of Spo07563.1 vs. UniProtKB/TrEMBL
Match: B9I132_POPTR (Subtilase family protein OS=Populus trichocarpa GN=POPTR_0011s03070g PE=4 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 1.400e-42
Identity = 94/152 (61.84%), Postives = 114/152 (75.00%), Query Frame = 1


		  
Query: 4   ISKILFLFFFLLPILAASSENQVYIVYLGGHKGDKEVHEIEQFHHSYLASVKETEQEARA 63
           + K+  LF FLLP+LA+  E QVYIVY G HKGDK +HEIE+FH SYL  VK+TE+EA A
Sbjct: 1   MKKLSILFLFLLPLLASCEEKQVYIVYFGEHKGDKALHEIEEFHQSYLYGVKQTEEEATA 60

Query: 64  SMLYSYKHSINGFAATLTPDQASKLSELEEVISVFRSHPMKHEMHTTRSWEFVGLEEGIK 123
           S+LYSYKHSINGFAA L PD+ASKLSEL+EV+SVF+S+P K+ + TTRSW F GLEE   
Sbjct: 61  SLLYSYKHSINGFAALLNPDEASKLSELKEVVSVFKSNPRKYSVQTTRSWRFAGLEEEGH 120

Query: 124 TTQMHSHTAGMTQGLLSKAKYGHNVIVGVLDS 156
                +H  G  + LL +A YG  VIVG+LDS
Sbjct: 121 NV---NHGFGGGRDLLKRAGYGKQVIVGLLDS 149

[Close]

BLAST of Spo07563.1 vs. ExPASy Swiss-Prot
Match: SBT56_ARATH (Subtilisin-like protease SBT5.6 OS=Arabidopsis thaliana GN=SBT5.6 PE=2 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 1.300e-44
Identity = 96/155 (61.94%), Postives = 115/155 (74.19%), Query Frame = 1


		  
Query: 8   LFLFFFLLPILAA-SSENQVYIVYLGGHKGDKEVHEIEQFHHSYLASVKETEQEARASML 67
           LF   FL+P+LA+ + E QVYIVY G HKGDK  HEIE+ HHSYL SVKE+E++ARAS+L
Sbjct: 7   LFPLLFLIPLLASCAEEKQVYIVYFGEHKGDKAFHEIEEHHHSYLQSVKESEEDARASLL 66

Query: 68  YSYKHSINGFAATLTPDQASKLSELEEVISVFRSHPMKHEMHTTRSWEFVGLEEGIKTTQ 127
           YSYKHSINGFAA LTPDQASKL +L EV+SVF+SHP K+E HTTRSWEFVGLEE    + 
Sbjct: 67  YSYKHSINGFAAELTPDQASKLEKLAEVVSVFKSHPRKYEAHTTRSWEFVGLEEEETDSD 126

Query: 128 MHSHTAG------MTQGLLSKAKYGHNVIVGVLDS 156
           +            + +  L KAK+G  +IVGVLDS
Sbjct: 127 VPRRKNDADDRFRVGRNFLKKAKHGDGIIVGVLDS 161

[Close]

BLAST of Spo07563.1 vs. ExPASy Swiss-Prot
Match: SBT55_ARATH (Subtilisin-like protease SBT5.5 OS=Arabidopsis thaliana GN=SBT5.5 PE=3 SV=2)

HSP 1 Score: 155.6 bits (392), Expect = 4.400e-37
Identity = 87/160 (54.37%), Postives = 109/160 (68.12%), Query Frame = 1


		  
Query: 8   LFLFFFLLPILAA-SSENQVYIVYLGGHKGDKEVHEIEQFHHSYLASVKETEQEARASML 67
           L L  FL+P+LA+ + E QVYIVY G HKGDK  HEIE  HHSYL SVKETE++A +S+L
Sbjct: 11  LSLLLFLVPLLASCTKEKQVYIVYFGEHKGDKAFHEIEAHHHSYLQSVKETEEDATSSLL 70

Query: 68  YSYKHSINGFAATLTPDQASKLSELEEVISVFRSHPMKHEMHTTRSWEFVGLEE------ 127
           Y    SINGFAA LTPDQAS+L EL+EV+SVF+S P K+++HTTRSWEFVGL+E      
Sbjct: 71  YRRASSINGFAAELTPDQASRLKELKEVVSVFKSDPRKYKIHTTRSWEFVGLKEEEGEDY 130

Query: 128 -GIKTTQMHSHTAG----MTQGLLSKAKYGHNVIVGVLDS 156
                   H +       + +  L  AK+G  VIVG++DS
Sbjct: 131 RSDGDAPRHKYDVNDRFRVGRKFLKNAKHGDGVIVGLIDS 170

[Close]

BLAST of Spo07563.1 vs. ExPASy Swiss-Prot
Match: SBT39_ARATH (Subtilisin-like protease SBT3.9 OS=Arabidopsis thaliana GN=SBT3.9 PE=3 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 7.800e-18
Identity = 59/154 (38.31%), Postives = 89/154 (57.79%), Query Frame = 1


		  
Query: 7   ILFLFFFLLPILAAS-----SENQVYIVYLGGHKGDKEVHEIEQFHHSYLASVKETEQEA 66
           ILFL  FL  +L        +E++VY+VYLG  + D     + + HH  L S+  +++  
Sbjct: 5   ILFLALFLSIVLNVQISFVVAESKVYVVYLGEKEHDNP-ESVTESHHQMLWSLLGSKEAV 64

Query: 67  RASMLYSYKHSINGFAATLTPDQASKLSELEEVISVFRSHPMKHEMHTTRSWEFVGLEEG 126
             S++YSY+H  +GFAA LT  QA ++SEL EV+ V  +    +EM TTR+W+++G+   
Sbjct: 65  LDSIVYSYRHGFSGFAAKLTESQAQQISELPEVVQVIPN--TLYEMTTTRTWDYLGV--- 124

Query: 127 IKTTQMHSHTAGMTQGLLSKAKYGHNVIVGVLDS 156
                    + G +  LL KA  G+NVIVGV+DS
Sbjct: 125 ---------SPGNSDSLLQKANMGYNVIVGVIDS 143

[Close]

BLAST of Spo07563.1 vs. ExPASy Swiss-Prot
Match: SBT3A_ARATH (Subtilisin-like protease SBT3.10 OS=Arabidopsis thaliana GN=SBT3.10 PE=3 SV=2)

HSP 1 Score: 89.7 bits (221), Expect = 3.000e-17
Identity = 57/154 (37.01%), Postives = 89/154 (57.79%), Query Frame = 1


		  
Query: 7   ILFLFFFLLPILAAS-----SENQVYIVYLGGHKGDKEVHEIEQFHHSYLASVKETEQEA 66
           I+ L FFL  +L        +E++VY+VYLG  + D     + + HH  L S+  +++  
Sbjct: 5   IILLAFFLSIVLNVQISFVVAESKVYVVYLGEKEHDNP-ESVTESHHQMLWSLLGSKEAV 64

Query: 67  RASMLYSYKHSINGFAATLTPDQASKLSELEEVISVFRSHPMKHEMHTTRSWEFVGLEEG 126
             S++YSY+H  +GFAA LT  QA ++SEL EV+ V  +    +EM TTR+W+++G+   
Sbjct: 65  LDSIVYSYRHGFSGFAAKLTESQAQQISELPEVVQVIPN--TLYEMTTTRTWDYLGV--- 124

Query: 127 IKTTQMHSHTAGMTQGLLSKAKYGHNVIVGVLDS 156
                    + G +  LL KA  G+NVIVGV+D+
Sbjct: 125 ---------SPGNSDSLLQKANMGYNVIVGVIDT 143

[Close]

BLAST of Spo07563.1 vs. ExPASy Swiss-Prot
Match: SBT3F_ARATH (Subtilisin-like protease SBT3.15 OS=Arabidopsis thaliana GN=SBT3.15 PE=2 SV=2)

HSP 1 Score: 89.4 bits (220), Expect = 3.900e-17
Identity = 60/155 (38.71%), Postives = 88/155 (56.77%), Query Frame = 1


		  
Query: 4   ISKILFL---FFFLLPILAASSENQVYIVYLGGHKGDKEVHEIEQFHHSYLASVKETEQE 63
           I+ +LFL     FL    A+ S ++VYIVYLG  + D +   +   HH  L S+ +++++
Sbjct: 16  IALVLFLNTELSFLTAEGASDSNSKVYIVYLGQREHD-DPELLTASHHQMLESLLQSKED 75

Query: 64  ARASMLYSYKHSINGFAATLTPDQASKLSELEEVISVFRSHPMKHEMHTTRSWEFVGLEE 123
           A  SM+YSY+H  +GFAA LT  QA K+SE  EVI V  +  +K  + TTR W+ +GL  
Sbjct: 76  AHNSMIYSYQHGFSGFAALLTSSQAKKISEHPEVIHVIPNRILK--LKTTRIWDHLGLSP 135

Query: 124 GIKTTQMHSHTAGMTQGLLSKAKYGHNVIVGVLDS 156
               T   S ++   +GLL     G   I+GV+DS
Sbjct: 136 --IPTSFSSSSSAKAKGLLHNTSMGSEAIIGVVDS 165

[Close]

BLAST of Spo07563.1 vs. TAIR (Arabidopsis)
Match: AT5G45650.1 (subtilase family protein)

HSP 1 Score: 180.6 bits (457), Expect = 7.200e-46
Identity = 96/155 (61.94%), Postives = 115/155 (74.19%), Query Frame = 1


		  
Query: 8   LFLFFFLLPILAA-SSENQVYIVYLGGHKGDKEVHEIEQFHHSYLASVKETEQEARASML 67
           LF   FL+P+LA+ + E QVYIVY G HKGDK  HEIE+ HHSYL SVKE+E++ARAS+L
Sbjct: 7   LFPLLFLIPLLASCAEEKQVYIVYFGEHKGDKAFHEIEEHHHSYLQSVKESEEDARASLL 66

Query: 68  YSYKHSINGFAATLTPDQASKLSELEEVISVFRSHPMKHEMHTTRSWEFVGLEEGIKTTQ 127
           YSYKHSINGFAA LTPDQASKL +L EV+SVF+SHP K+E HTTRSWEFVGLEE    + 
Sbjct: 67  YSYKHSINGFAAELTPDQASKLEKLAEVVSVFKSHPRKYEAHTTRSWEFVGLEEEETDSD 126

Query: 128 MHSHTAG------MTQGLLSKAKYGHNVIVGVLDS 156
           +            + +  L KAK+G  +IVGVLDS
Sbjct: 127 VPRRKNDADDRFRVGRNFLKKAKHGDGIIVGVLDS 161

[Close]

BLAST of Spo07563.1 vs. TAIR (Arabidopsis)
Match: AT4G10520.1 (Subtilase family protein)

HSP 1 Score: 91.7 bits (226), Expect = 4.400e-19
Identity = 59/154 (38.31%), Postives = 89/154 (57.79%), Query Frame = 1


		  
Query: 7   ILFLFFFLLPILAAS-----SENQVYIVYLGGHKGDKEVHEIEQFHHSYLASVKETEQEA 66
           ILFL  FL  +L        +E++VY+VYLG  + D     + + HH  L S+  +++  
Sbjct: 5   ILFLALFLSIVLNVQISFVVAESKVYVVYLGEKEHDNP-ESVTESHHQMLWSLLGSKEAV 64

Query: 67  RASMLYSYKHSINGFAATLTPDQASKLSELEEVISVFRSHPMKHEMHTTRSWEFVGLEEG 126
             S++YSY+H  +GFAA LT  QA ++SEL EV+ V  +    +EM TTR+W+++G+   
Sbjct: 65  LDSIVYSYRHGFSGFAAKLTESQAQQISELPEVVQVIPN--TLYEMTTTRTWDYLGV--- 124

Query: 127 IKTTQMHSHTAGMTQGLLSKAKYGHNVIVGVLDS 156
                    + G +  LL KA  G+NVIVGV+DS
Sbjct: 125 ---------SPGNSDSLLQKANMGYNVIVGVIDS 143

[Close]

BLAST of Spo07563.1 vs. TAIR (Arabidopsis)
Match: AT4G10530.1 (Subtilase family protein)

HSP 1 Score: 89.7 bits (221), Expect = 1.700e-18
Identity = 57/154 (37.01%), Postives = 89/154 (57.79%), Query Frame = 1


		  
Query: 7   ILFLFFFLLPILAAS-----SENQVYIVYLGGHKGDKEVHEIEQFHHSYLASVKETEQEA 66
           I+ L FFL  +L        +E++VY+VYLG  + D     + + HH  L S+  +++  
Sbjct: 5   IILLAFFLSIVLNVQISFVVAESKVYVVYLGEKEHDNP-ESVTESHHQMLWSLLGSKEAV 64

Query: 67  RASMLYSYKHSINGFAATLTPDQASKLSELEEVISVFRSHPMKHEMHTTRSWEFVGLEEG 126
             S++YSY+H  +GFAA LT  QA ++SEL EV+ V  +    +EM TTR+W+++G+   
Sbjct: 65  LDSIVYSYRHGFSGFAAKLTESQAQQISELPEVVQVIPN--TLYEMTTTRTWDYLGV--- 124

Query: 127 IKTTQMHSHTAGMTQGLLSKAKYGHNVIVGVLDS 156
                    + G +  LL KA  G+NVIVGV+D+
Sbjct: 125 ---------SPGNSDSLLQKANMGYNVIVGVIDT 143

[Close]

BLAST of Spo07563.1 vs. TAIR (Arabidopsis)
Match: AT4G21640.1 (Subtilase family protein)

HSP 1 Score: 89.4 bits (220), Expect = 2.200e-18
Identity = 60/155 (38.71%), Postives = 88/155 (56.77%), Query Frame = 1


		  
Query: 4   ISKILFL---FFFLLPILAASSENQVYIVYLGGHKGDKEVHEIEQFHHSYLASVKETEQE 63
           I+ +LFL     FL    A+ S ++VYIVYLG  + D +   +   HH  L S+ +++++
Sbjct: 16  IALVLFLNTELSFLTAEGASDSNSKVYIVYLGQREHD-DPELLTASHHQMLESLLQSKED 75

Query: 64  ARASMLYSYKHSINGFAATLTPDQASKLSELEEVISVFRSHPMKHEMHTTRSWEFVGLEE 123
           A  SM+YSY+H  +GFAA LT  QA K+SE  EVI V  +  +K  + TTR W+ +GL  
Sbjct: 76  AHNSMIYSYQHGFSGFAALLTSSQAKKISEHPEVIHVIPNRILK--LKTTRIWDHLGLSP 135

Query: 124 GIKTTQMHSHTAGMTQGLLSKAKYGHNVIVGVLDS 156
               T   S ++   +GLL     G   I+GV+DS
Sbjct: 136 --IPTSFSSSSSAKAKGLLHNTSMGSEAIIGVVDS 165

[Close]

BLAST of Spo07563.1 vs. TAIR (Arabidopsis)
Match: AT5G45640.1 (Subtilisin-like serine endopeptidase family protein)

HSP 1 Score: 88.2 bits (217), Expect = 4.900e-18
Identity = 56/118 (47.46%), Postives = 72/118 (61.02%), Query Frame = 1


		  
Query: 51  LASVKETEQ--EARASMLYSYKHSINGFAATLTPDQASKLSELEEVISVFRSHPMKHEMH 110
           LAS  + +Q  E RAS       SINGFAA LTPDQAS+L EL+EV+SVF+S P K+++H
Sbjct: 21  LASCTKEKQLREERAS-------SINGFAAELTPDQASRLKELKEVVSVFKSDPRKYKIH 80

Query: 111 TTRSWEFVGLEE-------GIKTTQMHSHTAG----MTQGLLSKAKYGHNVIVGVLDS 156
           TTRSWEFVGL+E              H +       + +  L  AK+G  VIVG++DS
Sbjct: 81  TTRSWEFVGLKEEEGEDYRSDGDAPRHKYDVNDRFRVGRKFLKNAKHGDGVIVGLIDS 131

The following BLAST results are available for this feature:

BLAST of Spo07563.1 vs. NCBI nr
BLAST of Spo07563.1 vs. UniProtKB/TrEMBL
BLAST of Spo07563.1 vs. ExPASy Swiss-Prot
BLAST of Spo07563.1 vs. TAIR (Arabidopsis)

BLAST of Spo07563.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902181395\|gb\|KNA09728.1\|	9.4e-80	100.	hypothetical protein SOVF_1509...	[more]
gi\|731370210\|ref\|XP_010665649.1\|	1.0e-57	76.7	PREDICTED: subtilisin-like pro...	[more]
gi\|743932273\|ref\|XP_011010424.1\|	1.4e-43	61.6	PREDICTED: subtilisin-like pro...	[more]
gi\|970010684\|ref\|XP_015066240.1\|	3.1e-43	58.7	PREDICTED: subtilisin-like pro...	[more]
gi\|743903976\|ref\|XP_011045349.1\|	4.1e-43	61.0	PREDICTED: subtilisin-like pro...	[more]

BLAST of Spo07563.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9QSR9_SPIOL	6.5e-80	100.	Uncharacterized protein OS=Spi...	[more]
A0A0J8B6Q9_BETVU	7.0e-58	76.7	Uncharacterized protein OS=Bet...	[more]
K4B8D4_SOLLC	1.1e-42	58.0	Uncharacterized protein OS=Sol...	[more]
F6HME6_VITVI	1.4e-42	59.4	Putative uncharacterized prote...	[more]
B9I132_POPTR	1.4e-42	61.8	Subtilase family protein OS=Po...	[more]

BLAST of Spo07563.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
SBT56_ARATH	1.3e-44	61.9	Subtilisin-like protease SBT5....	[more]
SBT55_ARATH	4.4e-37	54.3	Subtilisin-like protease SBT5....	[more]
SBT39_ARATH	7.8e-18	38.3	Subtilisin-like protease SBT3....	[more]
SBT3A_ARATH	3.0e-17	37.0	Subtilisin-like protease SBT3....	[more]
SBT3F_ARATH	3.9e-17	38.7	Subtilisin-like protease SBT3....	[more]

BLAST of Spo07563.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 5


Match Name	E-value	Identity	Description
AT5G45650.1	7.2e-46	61.9	subtilase family protein	[more]
AT4G10520.1	4.4e-19	38.3	Subtilase family protein	[more]
AT4G10530.1	1.7e-18	37.0	Subtilase family protein	[more]
AT4G21640.1	2.2e-18	38.7	Subtilase family protein	[more]
AT5G45640.1	4.9e-18	47.4	Subtilisin-like serine endopep...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000209	Peptidase S8/S53 domain	unknown	SSF52743	Subtilisin-like	coord: 4..155 score: 3.1
IPR010259	Peptidase S8 propeptide/proteinase inhibitor I9	GENE3D	3.30.70.80		coord: 27..97 score: 3.
IPR010259	Peptidase S8 propeptide/proteinase inhibitor I9	PFAM	PF05922	Inhibitor_I9	coord: 26..104 score: 5.8
IPR015500	Peptidase S8, subtilisin-related	PANTHER	PTHR10795	PROPROTEIN CONVERTASE SUBTILISIN/KEXIN	coord: 7..155 score: 7.3
None	No IPR available	PANTHER	PTHR10795:SF375	SUBTILISIN-LIKE SERINE ENDOPEPTIDASE FAMILY PROTEIN-RELATED	coord: 7..155 score: 7.3

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0005618	cell wall
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0004252	serine-type endopeptidase activity