Spo08276.1 (mRNA) | SpinachBase

Overview


Name	Spo08276.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	Protein kinase-like protein
Location	SpoScf_02820 : 29877 .. 30878 (-)
Sequence length	1002

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGTGGGTTAGAGGTGAGATTATAGGAAGGGGAAGCTTTGGTACAGTTAGTTTAGCAATTCCCAAATCAAATTCAAACAAATATGTTGTTAAATCGGCGGAACTTTCCAATTCTTTCTCTCTCAAAAACGAGAAAGAAATTCTTTCGGAGTTGAATCACCCAAACATTGTTCGATATTTGGGGGATTCTTTGAGTTTAGAGAAGGGTACACAGTTCTACAATCTATTCTTGGAGTATGCGACTAAAGGAAGTATAGCTGATCAACTTAAAGTTTCCGGTGGGAGATTCGAGTTTTCCGATGTGAAAAGGTATACCAAGTCCGTATTGAAGGGACTCTGTCATATTCATGAGAGTGGGTTTGTTCATTGCGATATTAAGCTTCAAAACGTTCTTCTTTTTCAAGATGGTACCGCGAAAATTGCTGATTTTGGATTAGCGAGGAGGGTTTCTGATGAGTTTGGGGAGCAATTGAGGGGAACTCCACTTTATATGTCGCCGGAGATTGTTTCCGGTGAGAAAGTTGAGTCTCCGGCGGATGTGTGGGCGCTTGGTTGTGCGGTTGTTGAGATGGTTACAGGTAAACCGGTGTGGGATTTGTTGCCGGAAAGTGATGTCAGCGCTTTGCTATACCGAATTGCGGTTGGCGGGGAATCTCCGAAGATTCCCGGGAATTTGTGCTCTGAAGGAAAAGATTTTCTTGGGAAGTGTTTTGTAAAAAATCCTAATGAGAGATGGACGGCCCAGATGTTGTTGGATCATCCATTTGTTTCCGGGTTGAAAGATGAACGGCCTTCTGTGTCTCCTAAATGCCCGTTTGAGTTCCCTGATTGGCAATCGAATTCGTGTTCGGATTCGGAATCAGTATCATCTTTCGGGTTTTCAGAAGATTGTTGTTGGGCTTCATCTTGGAGCCCCGTTTCACGTACATTAGACGAAGAATTGATTTCTAATCGGATACCCAATTGGAATGATTCGGATGATTGGGTTACCGTTAGATAA

mRNA sequence

ATGGAGTGGGTTAGAGGTGAGATTATAGGAAGGGGAAGCTTTGGTACAGTTAGTTTAGCAATTCCCAAATCAAATTCAAACAAATATGTTGTTAAATCGGCGGAACTTTCCAATTCTTTCTCTCTCAAAAACGAGAAAGAAATTCTTTCGGAGTTGAATCACCCAAACATTGTTCGATATTTGGGGGATTCTTTGAGTTTAGAGAAGGGTACACAGTTCTACAATCTATTCTTGGAGTATGCGACTAAAGGAAGTATAGCTGATCAACTTAAAGTTTCCGGTGGGAGATTCGAGTTTTCCGATGTGAAAAGGTATACCAAGTCCGTATTGAAGGGACTCTGTCATATTCATGAGAGTGGGTTTGTTCATTGCGATATTAAGCTTCAAAACGTTCTTCTTTTTCAAGATGGTACCGCGAAAATTGCTGATTTTGGATTAGCGAGGAGGGTTTCTGATGAGTTTGGGGAGCAATTGAGGGGAACTCCACTTTATATGTCGCCGGAGATTGTTTCCGGTGAGAAAGTTGAGTCTCCGGCGGATGTGTGGGCGCTTGGTTGTGCGGTTGTTGAGATGGTTACAGGTAAACCGGTGTGGGATTTGTTGCCGGAAAGTGATGTCAGCGCTTTGCTATACCGAATTGCGGTTGGCGGGGAATCTCCGAAGATTCCCGGGAATTTGTGCTCTGAAGGAAAAGATTTTCTTGGGAAGTGTTTTGTAAAAAATCCTAATGAGAGATGGACGGCCCAGATGTTGTTGGATCATCCATTTGTTTCCGGGTTGAAAGATGAACGGCCTTCTGTGTCTCCTAAATGCCCGTTTGAGTTCCCTGATTGGCAATCGAATTCGTGTTCGGATTCGGAATCAGTATCATCTTTCGGGTTTTCAGAAGATTGTTGTTGGGCTTCATCTTGGAGCCCCGTTTCACGTACATTAGACGAAGAATTGATTTCTAATCGGATACCCAATTGGAATGATTCGGATGATTGGGTTACCGTTAGATAA

Coding sequence (CDS)

ATGGAGTGGGTTAGAGGTGAGATTATAGGAAGGGGAAGCTTTGGTACAGTTAGTTTAGCAATTCCCAAATCAAATTCAAACAAATATGTTGTTAAATCGGCGGAACTTTCCAATTCTTTCTCTCTCAAAAACGAGAAAGAAATTCTTTCGGAGTTGAATCACCCAAACATTGTTCGATATTTGGGGGATTCTTTGAGTTTAGAGAAGGGTACACAGTTCTACAATCTATTCTTGGAGTATGCGACTAAAGGAAGTATAGCTGATCAACTTAAAGTTTCCGGTGGGAGATTCGAGTTTTCCGATGTGAAAAGGTATACCAAGTCCGTATTGAAGGGACTCTGTCATATTCATGAGAGTGGGTTTGTTCATTGCGATATTAAGCTTCAAAACGTTCTTCTTTTTCAAGATGGTACCGCGAAAATTGCTGATTTTGGATTAGCGAGGAGGGTTTCTGATGAGTTTGGGGAGCAATTGAGGGGAACTCCACTTTATATGTCGCCGGAGATTGTTTCCGGTGAGAAAGTTGAGTCTCCGGCGGATGTGTGGGCGCTTGGTTGTGCGGTTGTTGAGATGGTTACAGGTAAACCGGTGTGGGATTTGTTGCCGGAAAGTGATGTCAGCGCTTTGCTATACCGAATTGCGGTTGGCGGGGAATCTCCGAAGATTCCCGGGAATTTGTGCTCTGAAGGAAAAGATTTTCTTGGGAAGTGTTTTGTAAAAAATCCTAATGAGAGATGGACGGCCCAGATGTTGTTGGATCATCCATTTGTTTCCGGGTTGAAAGATGAACGGCCTTCTGTGTCTCCTAAATGCCCGTTTGAGTTCCCTGATTGGCAATCGAATTCGTGTTCGGATTCGGAATCAGTATCATCTTTCGGGTTTTCAGAAGATTGTTGTTGGGCTTCATCTTGGAGCCCCGTTTCACGTACATTAGACGAAGAATTGATTTCTAATCGGATACCCAATTGGAATGATTCGGATGATTGGGTTACCGTTAGATAA

Protein sequence

MEWVRGEIIGRGSFGTVSLAIPKSNSNKYVVKSAELSNSFSLKNEKEILSELNHPNIVRYLGDSLSLEKGTQFYNLFLEYATKGSIADQLKVSGGRFEFSDVKRYTKSVLKGLCHIHESGFVHCDIKLQNVLLFQDGTAKIADFGLARRVSDEFGEQLRGTPLYMSPEIVSGEKVESPADVWALGCAVVEMVTGKPVWDLLPESDVSALLYRIAVGGESPKIPGNLCSEGKDFLGKCFVKNPNERWTAQMLLDHPFVSGLKDERPSVSPKCPFEFPDWQSNSCSDSESVSSFGFSEDCCWASSWSPVSRTLDEELISNRIPNWNDSDDWVTVR

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo08276	Spo08276	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo08276.1	Spo08276.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo08276.1.CDS.1	Spo08276.1.CDS.1	CDS

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo08276.1.exon.1	Spo08276.1.exon.1	exon

Homology

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BLAST of Spo08276.1 vs. NCBI nr
Match: gi|902176008|gb|KNA08706.1| (hypothetical protein SOVF_160260 [Spinacia oleracea])

HSP 1 Score: 689.9 bits (1779), Expect = 2.200e-195
Identity = 333/333 (100.00%), Postives = 333/333 (100.00%), Query Frame = 1


		  
Query: 1   MEWVRGEIIGRGSFGTVSLAIPKSNSNKYVVKSAELSNSFSLKNEKEILSELNHPNIVRY 60
           MEWVRGEIIGRGSFGTVSLAIPKSNSNKYVVKSAELSNSFSLKNEKEILSELNHPNIVRY
Sbjct: 1   MEWVRGEIIGRGSFGTVSLAIPKSNSNKYVVKSAELSNSFSLKNEKEILSELNHPNIVRY 60

Query: 61  LGDSLSLEKGTQFYNLFLEYATKGSIADQLKVSGGRFEFSDVKRYTKSVLKGLCHIHESG 120
           LGDSLSLEKGTQFYNLFLEYATKGSIADQLKVSGGRFEFSDVKRYTKSVLKGLCHIHESG
Sbjct: 61  LGDSLSLEKGTQFYNLFLEYATKGSIADQLKVSGGRFEFSDVKRYTKSVLKGLCHIHESG 120

Query: 121 FVHCDIKLQNVLLFQDGTAKIADFGLARRVSDEFGEQLRGTPLYMSPEIVSGEKVESPAD 180
           FVHCDIKLQNVLLFQDGTAKIADFGLARRVSDEFGEQLRGTPLYMSPEIVSGEKVESPAD
Sbjct: 121 FVHCDIKLQNVLLFQDGTAKIADFGLARRVSDEFGEQLRGTPLYMSPEIVSGEKVESPAD 180

Query: 181 VWALGCAVVEMVTGKPVWDLLPESDVSALLYRIAVGGESPKIPGNLCSEGKDFLGKCFVK 240
           VWALGCAVVEMVTGKPVWDLLPESDVSALLYRIAVGGESPKIPGNLCSEGKDFLGKCFVK
Sbjct: 181 VWALGCAVVEMVTGKPVWDLLPESDVSALLYRIAVGGESPKIPGNLCSEGKDFLGKCFVK 240

Query: 241 NPNERWTAQMLLDHPFVSGLKDERPSVSPKCPFEFPDWQSNSCSDSESVSSFGFSEDCCW 300
           NPNERWTAQMLLDHPFVSGLKDERPSVSPKCPFEFPDWQSNSCSDSESVSSFGFSEDCCW
Sbjct: 241 NPNERWTAQMLLDHPFVSGLKDERPSVSPKCPFEFPDWQSNSCSDSESVSSFGFSEDCCW 300

Query: 301 ASSWSPVSRTLDEELISNRIPNWNDSDDWVTVR 334
           ASSWSPVSRTLDEELISNRIPNWNDSDDWVTVR
Sbjct: 301 ASSWSPVSRTLDEELISNRIPNWNDSDDWVTVR 333

[Close]

BLAST of Spo08276.1 vs. NCBI nr
Match: gi|731375935|ref|XP_010667409.1| (PREDICTED: mitogen-activated protein kinase kinase kinase A [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 528.5 bits (1360), Expect = 8.600e-147
Identity = 255/345 (73.91%), Postives = 297/345 (86.09%), Query Frame = 1


		  
Query: 1   MEWVRGEIIGRGSFGTVSLAIPKS-NSNKYVVKSAELSNSFSLKNEKEILSEL--NHPNI 60
           MEWVRGEIIGRGSFGTV+LAIPKS NS ++VVKS+E+S   SLKNE+EIL EL  NHPNI
Sbjct: 1   MEWVRGEIIGRGSFGTVNLAIPKSSNSTQFVVKSSEISKCSSLKNEREILLELSNNHPNI 60

Query: 61  VRYLGDSLSLEKGTQFYNLFLEYATKGSIADQLKVSGGRFEFSDVKRYTKSVLKGLCHIH 120
           ++YLGDSLS+EKGTQ YNLFLEYA+KGS+AD LK  GG+FEFSDVK YTKS+LKGLCHIH
Sbjct: 61  IQYLGDSLSIEKGTQIYNLFLEYASKGSLADHLKFCGGKFEFSDVKSYTKSILKGLCHIH 120

Query: 121 ESGFVHCDIKLQNVLLFQDGTAKIADFGLARRVSDEFGEQLRGTPLYMSPEIVSGEKVES 180
           ++GFVHCDIKLQN+LL  + T KIADFGLARRVSDEFG+QLRGTPLYMSPEI++GEKVES
Sbjct: 121 QNGFVHCDIKLQNILLLDNTTIKIADFGLARRVSDEFGKQLRGTPLYMSPEIIAGEKVES 180

Query: 181 PADVWALGCAVVEMVTGKPVWDLLPESDVSALLYRIAVGGESPKIPGNLCSEGKDFLGKC 240
           PAD+WALGCAV+EM +G   W++ PESDVSALLYRIAVGGESPKIPGNLC EGKDFLGKC
Sbjct: 181 PADIWALGCAVIEMFSGISAWEMSPESDVSALLYRIAVGGESPKIPGNLCCEGKDFLGKC 240

Query: 241 FVKNPNERWTAQMLLDHPFVSGLKDERPS---VSPKCPFEFPDWQ------SNSCSDSES 300
           F+ NPNERWTAQMLL+HPF+SGL D++     +SP+CPFEFPDWQ      S S SDSES
Sbjct: 241 FINNPNERWTAQMLLEHPFLSGLYDDKQQQSFISPRCPFEFPDWQSTSGSSSGSSSDSES 300

Query: 301 VSSFGFSEDCCWASSWSPVSRTLDEELISNRIPNWNDSDDWVTVR 334
           +SSFGFSE+  W  + SP+S  L+ +LIS+RIP+W  S+DWVT+R
Sbjct: 301 ISSFGFSEE-SWTLTSSPISHRLEGKLISDRIPDWGASNDWVTIR 344

[Close]

BLAST of Spo08276.1 vs. NCBI nr
Match: gi|1009144451|ref|XP_015889808.1| (PREDICTED: mitogen-activated protein kinase kinase kinase NPK1 [Ziziphus jujuba])

HSP 1 Score: 359.8 bits (922), Expect = 5.300e-96
Identity = 187/356 (52.53%), Postives = 244/356 (68.54%), Query Frame = 1


		  
Query: 1   MEWVRGEIIGRGSFGTVSLAIPKSNSNKY----VVKSAELSNSFSLKNEKEILSEL-NHP 60
           MEWVRGE IG GSF T+SLA+P+  S +      VKS+E+ NS S+ NEKEIL +L + P
Sbjct: 1   MEWVRGEEIGSGSFATISLAVPRKASAEIPPLMAVKSSEIFNSASINNEKEILDQLADCP 60

Query: 61  NIVRYLGDSLSLEKGTQFYNLFLEYATKGSIADQLKVSGGRFEFSDVKRYTKSVLKGLCH 120
            ++R  G++ S EKG +F+NL LEYA+ GS+ DQLK +GGR   SD++RYT+++LKGL  
Sbjct: 61  QVIRCFGENYSTEKGEEFHNLMLEYASGGSLVDQLKKNGGRLSESDIRRYTRTILKGLSF 120

Query: 121 IHESGFVHCDIKLQNVLLFQDGTAKIADFGLARRVSDEFGE-QLRGTPLYMSPEIVSGEK 180
           IH  GFVHCDIKLQN+L+F++G AKIADFGLA++  ++  E +LRGTPLYMSPE V+  +
Sbjct: 121 IHTKGFVHCDIKLQNILVFENGAAKIADFGLAKKTGEKKTEVELRGTPLYMSPESVNDNE 180

Query: 181 VESPADVWALGCAVVEMVTGKPVWDLLPESDVSALLYRIAVGGESPKIPGNLCSEGKDFL 240
            ESP+D+WALGCAVVEM+TGKP W+  P S++ ALL RI VG E P++P  L  EGKDFL
Sbjct: 181 YESPSDIWALGCAVVEMMTGKPAWNCEPGSNICALLIRIGVGEELPQVPEELSEEGKDFL 240

Query: 241 GKCFVKNPNERWTAQMLLDHPFVSG---LKDERPSVSPKCPFEFPDWQSNSCS------- 300
           G+CFVK+P +RWTA  LL HPF+S    +  E  S SP+ PF+FP+W S   +       
Sbjct: 241 GRCFVKDPRKRWTADRLLKHPFISDDDTVSLEELSTSPRSPFDFPEWVSMQSTVRFSKLS 300

Query: 301 --DSESVSSFGFSEDCCWASSWSPVSRTLDEE-----LISNRIPNWNDSDDWVTVR 334
               ES + FG   D  + SS+S  S +   E     L+S+  P W  SD WVTVR
Sbjct: 301 EFSPESENWFGRELDSYFGSSYSSSSSSTCLENRLRRLMSDETPYWLVSDSWVTVR 356

[Close]

BLAST of Spo08276.1 vs. NCBI nr
Match: gi|823139394|ref|XP_012469556.1| (PREDICTED: mitogen-activated protein kinase kinase kinase YODA-like [Gossypium raimondii])

HSP 1 Score: 355.5 bits (911), Expect = 9.900e-95
Identity = 181/356 (50.84%), Postives = 237/356 (66.57%), Query Frame = 1


		  
Query: 1   MEWVRGEIIGRGSFGTVSLAIPK---SNSNKYVVKSAELSNSFSLKNEKEILSELNH-PN 60
           MEWVRG+ IG GSFGTV+L +PK   S S    VKS E+ +S SLKNEKE+L  +   P 
Sbjct: 1   MEWVRGDTIGSGSFGTVNLVVPKNGCSRSPLAAVKSCEVIDSVSLKNEKEVLDRIGSCPQ 60

Query: 61  IVRYLGDSLSLEKGTQFYNLFLEYATKGSIADQLKVSGGRFEFSDVKRYTKSVLKGLCHI 120
           IVR  GD  ++EKG + YNLFLEYA+KGS+AD++K SGG+   SDVKRY KS+LKGL  +
Sbjct: 61  IVRCFGDDYTVEKGVKLYNLFLEYASKGSLADEVKKSGGKLPESDVKRYGKSILKGLRFV 120

Query: 121 HESGFVHCDIKLQNVLLFQDGTAKIADFGLARRVSDEFGEQLRGTPLYMSPEIVSGEKVE 180
           H  GFVHCD+KL+NVLLF +G  K+ADFGLA+R   + G ++RGTPL ++PE V+    +
Sbjct: 121 HSKGFVHCDVKLRNVLLFGNGDVKLADFGLAKRNGGKQGMEIRGTPLNLAPESVNENSYD 180

Query: 181 SPADVWALGCAVVEMVTGKPVWDLLPESDVSALLYRIAVGGESPKIPGNLCSEGKDFLGK 240
           SP D+WA GCA+VEM TGKP W+  P ++++ LL +I V  E P+IP  L  EGKDFLGK
Sbjct: 181 SPVDIWAFGCAIVEMFTGKPAWNFEPGTNMAGLLIKIGVSDEVPEIPLELSEEGKDFLGK 240

Query: 241 CFVKNPNERWTAQMLLDHPFVSG--------LKDERPSV---------SPKCPFEFPDWQ 300
           CFVK+P  RWTA+MLLDHPF++G         ++E  S+         SP+CPF+FPDW 
Sbjct: 241 CFVKDPKNRWTAEMLLDHPFMAGDEIISLNRCQEEEESISCSFEEFSASPRCPFDFPDW- 300

Query: 301 SNSCSDSESVSSFGFSEDCCWASSWSPVSRTLD--EELISNRIPNWNDSDDWVTVR 334
               S   +VSS+              VS  LD   EL+ + +PNW  S++W+T+R
Sbjct: 301 ---VSTESTVSSY--------------VSSGLDRVRELVCDEVPNWLASENWITLR 338

[Close]

BLAST of Spo08276.1 vs. NCBI nr
Match: gi|590704879|ref|XP_007047281.1| (Mitogen-activated protein kinase kinase kinase 19, putative [Theobroma cacao])

HSP 1 Score: 354.0 bits (907), Expect = 2.900e-94
Identity = 183/361 (50.69%), Postives = 240/361 (66.48%), Query Frame = 1


		  
Query: 1   MEWVRGEIIGRGSFGTVSLAIPKSNSNK----YVVKSAELSNSFSLKNEKEILSELNH-P 60
           M+WVRG+ IG GSFGTV+L +PK   +K      VKS E   S SLK+EKE+L +L   P
Sbjct: 1   MDWVRGDTIGCGSFGTVNLVLPKKGFSKSPSVMAVKSCETIKSVSLKSEKEVLDQLGFCP 60

Query: 61  NIVRYLGDSLSLEKGTQFYNLFLEYATKGSIADQLKVSGGRFEFSDVKRYTKSVLKGLCH 120
            I+R  GD  +LE G   YNLFLEYA KGS+ADQ+K SGG    SDV+RY +S+LKGL  
Sbjct: 61  QIIRCFGDGYTLENGESLYNLFLEYADKGSLADQVKKSGGNLIESDVRRYARSILKGLLF 120

Query: 121 IHESGFVHCDIKLQNVLLFQDGTAKIADFGLARRVSDEFGEQ-----LRGTPLYMSPEIV 180
           +H  GF HCDIK+QNVLLF +G  KI DFGLA+R+ +E G++     +RGTPL ++PE V
Sbjct: 121 VHAKGFAHCDIKVQNVLLFGNGNVKITDFGLAKRIGEEEGDEPRRIEIRGTPLNIAPESV 180

Query: 181 SGEKVESPADVWALGCAVVEMVTGKPVWDLLPESDVSALLYRIAVGGESPKIPGNLCSEG 240
           +  + +SP D+WALGC +VEM TGKP W+L P ++++ALL +I V  E P+IP  L  +G
Sbjct: 181 NDNEYDSPVDIWALGCVIVEMFTGKPAWNLKPGTNLAALLIKIGVSDELPEIPAELSEDG 240

Query: 241 KDFLGKCFVKNPNERWTAQMLLDHPFVS------GLKDERP---------SVSPKCPFEF 300
           KDFL KCFVK+PN RWTA+MLLDHPFV+       L++E P         S+SP+CPF+F
Sbjct: 241 KDFLEKCFVKDPNNRWTAEMLLDHPFVAADDETVALEEESPSPNCSCEEFSMSPRCPFDF 300

Query: 301 PDWQSNSCSDSESVSSFGFSEDCCWASSWSP-VSRTLD--EELISNRIPNWNDSDDWVTV 334
           PDW S   + S   S   F E+    SS+S  +S  LD   +L  ++ PNW+ S  W+T+
Sbjct: 301 PDWVSTQYTAS---SQSTFQENSSLVSSFSSYLSSPLDRIHQLACHQAPNWSVSGSWITL 358

[Close]

BLAST of Spo08276.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QQB3_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_160260 PE=3 SV=1)

HSP 1 Score: 689.9 bits (1779), Expect = 1.500e-195
Identity = 333/333 (100.00%), Postives = 333/333 (100.00%), Query Frame = 1


		  
Query: 1   MEWVRGEIIGRGSFGTVSLAIPKSNSNKYVVKSAELSNSFSLKNEKEILSELNHPNIVRY 60
           MEWVRGEIIGRGSFGTVSLAIPKSNSNKYVVKSAELSNSFSLKNEKEILSELNHPNIVRY
Sbjct: 1   MEWVRGEIIGRGSFGTVSLAIPKSNSNKYVVKSAELSNSFSLKNEKEILSELNHPNIVRY 60

Query: 61  LGDSLSLEKGTQFYNLFLEYATKGSIADQLKVSGGRFEFSDVKRYTKSVLKGLCHIHESG 120
           LGDSLSLEKGTQFYNLFLEYATKGSIADQLKVSGGRFEFSDVKRYTKSVLKGLCHIHESG
Sbjct: 61  LGDSLSLEKGTQFYNLFLEYATKGSIADQLKVSGGRFEFSDVKRYTKSVLKGLCHIHESG 120

Query: 121 FVHCDIKLQNVLLFQDGTAKIADFGLARRVSDEFGEQLRGTPLYMSPEIVSGEKVESPAD 180
           FVHCDIKLQNVLLFQDGTAKIADFGLARRVSDEFGEQLRGTPLYMSPEIVSGEKVESPAD
Sbjct: 121 FVHCDIKLQNVLLFQDGTAKIADFGLARRVSDEFGEQLRGTPLYMSPEIVSGEKVESPAD 180

Query: 181 VWALGCAVVEMVTGKPVWDLLPESDVSALLYRIAVGGESPKIPGNLCSEGKDFLGKCFVK 240
           VWALGCAVVEMVTGKPVWDLLPESDVSALLYRIAVGGESPKIPGNLCSEGKDFLGKCFVK
Sbjct: 181 VWALGCAVVEMVTGKPVWDLLPESDVSALLYRIAVGGESPKIPGNLCSEGKDFLGKCFVK 240

Query: 241 NPNERWTAQMLLDHPFVSGLKDERPSVSPKCPFEFPDWQSNSCSDSESVSSFGFSEDCCW 300
           NPNERWTAQMLLDHPFVSGLKDERPSVSPKCPFEFPDWQSNSCSDSESVSSFGFSEDCCW
Sbjct: 241 NPNERWTAQMLLDHPFVSGLKDERPSVSPKCPFEFPDWQSNSCSDSESVSSFGFSEDCCW 300

Query: 301 ASSWSPVSRTLDEELISNRIPNWNDSDDWVTVR 334
           ASSWSPVSRTLDEELISNRIPNWNDSDDWVTVR
Sbjct: 301 ASSWSPVSRTLDEELISNRIPNWNDSDDWVTVR 333

[Close]

BLAST of Spo08276.1 vs. UniProtKB/TrEMBL
Match: A0A0J8B321_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_008540 PE=3 SV=1)

HSP 1 Score: 528.5 bits (1360), Expect = 6.000e-147
Identity = 255/345 (73.91%), Postives = 297/345 (86.09%), Query Frame = 1


		  
Query: 1   MEWVRGEIIGRGSFGTVSLAIPKS-NSNKYVVKSAELSNSFSLKNEKEILSEL--NHPNI 60
           MEWVRGEIIGRGSFGTV+LAIPKS NS ++VVKS+E+S   SLKNE+EIL EL  NHPNI
Sbjct: 1   MEWVRGEIIGRGSFGTVNLAIPKSSNSTQFVVKSSEISKCSSLKNEREILLELSNNHPNI 60

Query: 61  VRYLGDSLSLEKGTQFYNLFLEYATKGSIADQLKVSGGRFEFSDVKRYTKSVLKGLCHIH 120
           ++YLGDSLS+EKGTQ YNLFLEYA+KGS+AD LK  GG+FEFSDVK YTKS+LKGLCHIH
Sbjct: 61  IQYLGDSLSIEKGTQIYNLFLEYASKGSLADHLKFCGGKFEFSDVKSYTKSILKGLCHIH 120

Query: 121 ESGFVHCDIKLQNVLLFQDGTAKIADFGLARRVSDEFGEQLRGTPLYMSPEIVSGEKVES 180
           ++GFVHCDIKLQN+LL  + T KIADFGLARRVSDEFG+QLRGTPLYMSPEI++GEKVES
Sbjct: 121 QNGFVHCDIKLQNILLLDNTTIKIADFGLARRVSDEFGKQLRGTPLYMSPEIIAGEKVES 180

Query: 181 PADVWALGCAVVEMVTGKPVWDLLPESDVSALLYRIAVGGESPKIPGNLCSEGKDFLGKC 240
           PAD+WALGCAV+EM +G   W++ PESDVSALLYRIAVGGESPKIPGNLC EGKDFLGKC
Sbjct: 181 PADIWALGCAVIEMFSGISAWEMSPESDVSALLYRIAVGGESPKIPGNLCCEGKDFLGKC 240

Query: 241 FVKNPNERWTAQMLLDHPFVSGLKDERPS---VSPKCPFEFPDWQ------SNSCSDSES 300
           F+ NPNERWTAQMLL+HPF+SGL D++     +SP+CPFEFPDWQ      S S SDSES
Sbjct: 241 FINNPNERWTAQMLLEHPFLSGLYDDKQQQSFISPRCPFEFPDWQSTSGSSSGSSSDSES 300

Query: 301 VSSFGFSEDCCWASSWSPVSRTLDEELISNRIPNWNDSDDWVTVR 334
           +SSFGFSE+  W  + SP+S  L+ +LIS+RIP+W  S+DWVT+R
Sbjct: 301 ISSFGFSEE-SWTLTSSPISHRLEGKLISDRIPDWGASNDWVTIR 344

[Close]

BLAST of Spo08276.1 vs. UniProtKB/TrEMBL
Match: A0A0D2QEQ1_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_003G025400 PE=3 SV=1)

HSP 1 Score: 355.5 bits (911), Expect = 6.900e-95
Identity = 181/356 (50.84%), Postives = 237/356 (66.57%), Query Frame = 1


		  
Query: 1   MEWVRGEIIGRGSFGTVSLAIPK---SNSNKYVVKSAELSNSFSLKNEKEILSELNH-PN 60
           MEWVRG+ IG GSFGTV+L +PK   S S    VKS E+ +S SLKNEKE+L  +   P 
Sbjct: 1   MEWVRGDTIGSGSFGTVNLVVPKNGCSRSPLAAVKSCEVIDSVSLKNEKEVLDRIGSCPQ 60

Query: 61  IVRYLGDSLSLEKGTQFYNLFLEYATKGSIADQLKVSGGRFEFSDVKRYTKSVLKGLCHI 120
           IVR  GD  ++EKG + YNLFLEYA+KGS+AD++K SGG+   SDVKRY KS+LKGL  +
Sbjct: 61  IVRCFGDDYTVEKGVKLYNLFLEYASKGSLADEVKKSGGKLPESDVKRYGKSILKGLRFV 120

Query: 121 HESGFVHCDIKLQNVLLFQDGTAKIADFGLARRVSDEFGEQLRGTPLYMSPEIVSGEKVE 180
           H  GFVHCD+KL+NVLLF +G  K+ADFGLA+R   + G ++RGTPL ++PE V+    +
Sbjct: 121 HSKGFVHCDVKLRNVLLFGNGDVKLADFGLAKRNGGKQGMEIRGTPLNLAPESVNENSYD 180

Query: 181 SPADVWALGCAVVEMVTGKPVWDLLPESDVSALLYRIAVGGESPKIPGNLCSEGKDFLGK 240
           SP D+WA GCA+VEM TGKP W+  P ++++ LL +I V  E P+IP  L  EGKDFLGK
Sbjct: 181 SPVDIWAFGCAIVEMFTGKPAWNFEPGTNMAGLLIKIGVSDEVPEIPLELSEEGKDFLGK 240

Query: 241 CFVKNPNERWTAQMLLDHPFVSG--------LKDERPSV---------SPKCPFEFPDWQ 300
           CFVK+P  RWTA+MLLDHPF++G         ++E  S+         SP+CPF+FPDW 
Sbjct: 241 CFVKDPKNRWTAEMLLDHPFMAGDEIISLNRCQEEEESISCSFEEFSASPRCPFDFPDW- 300

Query: 301 SNSCSDSESVSSFGFSEDCCWASSWSPVSRTLD--EELISNRIPNWNDSDDWVTVR 334
               S   +VSS+              VS  LD   EL+ + +PNW  S++W+T+R
Sbjct: 301 ---VSTESTVSSY--------------VSSGLDRVRELVCDEVPNWLASENWITLR 338

[Close]

BLAST of Spo08276.1 vs. UniProtKB/TrEMBL
Match: A0A061DN83_THECC (Mitogen-activated protein kinase kinase kinase 19, putative OS=Theobroma cacao GN=TCM_000635 PE=3 SV=1)

HSP 1 Score: 354.0 bits (907), Expect = 2.000e-94
Identity = 183/361 (50.69%), Postives = 240/361 (66.48%), Query Frame = 1


		  
Query: 1   MEWVRGEIIGRGSFGTVSLAIPKSNSNK----YVVKSAELSNSFSLKNEKEILSELNH-P 60
           M+WVRG+ IG GSFGTV+L +PK   +K      VKS E   S SLK+EKE+L +L   P
Sbjct: 1   MDWVRGDTIGCGSFGTVNLVLPKKGFSKSPSVMAVKSCETIKSVSLKSEKEVLDQLGFCP 60

Query: 61  NIVRYLGDSLSLEKGTQFYNLFLEYATKGSIADQLKVSGGRFEFSDVKRYTKSVLKGLCH 120
            I+R  GD  +LE G   YNLFLEYA KGS+ADQ+K SGG    SDV+RY +S+LKGL  
Sbjct: 61  QIIRCFGDGYTLENGESLYNLFLEYADKGSLADQVKKSGGNLIESDVRRYARSILKGLLF 120

Query: 121 IHESGFVHCDIKLQNVLLFQDGTAKIADFGLARRVSDEFGEQ-----LRGTPLYMSPEIV 180
           +H  GF HCDIK+QNVLLF +G  KI DFGLA+R+ +E G++     +RGTPL ++PE V
Sbjct: 121 VHAKGFAHCDIKVQNVLLFGNGNVKITDFGLAKRIGEEEGDEPRRIEIRGTPLNIAPESV 180

Query: 181 SGEKVESPADVWALGCAVVEMVTGKPVWDLLPESDVSALLYRIAVGGESPKIPGNLCSEG 240
           +  + +SP D+WALGC +VEM TGKP W+L P ++++ALL +I V  E P+IP  L  +G
Sbjct: 181 NDNEYDSPVDIWALGCVIVEMFTGKPAWNLKPGTNLAALLIKIGVSDELPEIPAELSEDG 240

Query: 241 KDFLGKCFVKNPNERWTAQMLLDHPFVS------GLKDERP---------SVSPKCPFEF 300
           KDFL KCFVK+PN RWTA+MLLDHPFV+       L++E P         S+SP+CPF+F
Sbjct: 241 KDFLEKCFVKDPNNRWTAEMLLDHPFVAADDETVALEEESPSPNCSCEEFSMSPRCPFDF 300

Query: 301 PDWQSNSCSDSESVSSFGFSEDCCWASSWSP-VSRTLD--EELISNRIPNWNDSDDWVTV 334
           PDW S   + S   S   F E+    SS+S  +S  LD   +L  ++ PNW+ S  W+T+
Sbjct: 301 PDWVSTQYTAS---SQSTFQENSSLVSSFSSYLSSPLDRIHQLACHQAPNWSVSGSWITL 358

[Close]

BLAST of Spo08276.1 vs. UniProtKB/TrEMBL
Match: A0A072TH41_MEDTR (MAP3K-like kinase OS=Medicago truncatula GN=MTR_0154s0020 PE=3 SV=1)

HSP 1 Score: 345.9 bits (886), Expect = 5.500e-92
Identity = 177/339 (52.21%), Postives = 241/339 (71.09%), Query Frame = 1


		  
Query: 1   MEWVRGEIIGRGSFGTVSLAIPKSNSNKY--VVKSAELSNSFSLKNEKEILSELNH-PNI 60
           M+WVRGE +GRGSF TV+L IPKSNSN     VK++E+S S SLKNEK +L +L     I
Sbjct: 1   MDWVRGETVGRGSFATVNLVIPKSNSNSTPTAVKTSEVSTSSSLKNEKYVLHQLGSCQRI 60

Query: 61  VRYLGDSLSLEKGTQFYNLFLEYATKGSIADQLKVSGGRFEFSDVKRYTKSVLKGLCHIH 120
           +   GD  + E G ++YNLFLEYA+ GS++DQ+K++GGR     ++RYT+S+++GL HIH
Sbjct: 61  IPCFGDDYTFENGKEYYNLFLEYASAGSLSDQVKLNGGRIPEQHIRRYTRSIVEGLDHIH 120

Query: 121 ESGFVHCDIKLQNVLLFQDGTAKIADFGLARRVSDEFGEQLRGTPLYMSPEIVSGEKVES 180
            +GFVHCDIKLQN+L+F DG  KIADFGLA++  ++   + RGTPL+MSPE V+  + ES
Sbjct: 121 RNGFVHCDIKLQNILVFNDGEIKIADFGLAKKTGEKQSFECRGTPLFMSPESVNNGEHES 180

Query: 181 PADVWALGCAVVEMVTGKPVWDLLPESDVSALLYRIAVGGESPKIPGNLCSEGKDFLGKC 240
           PAD+WALGCAVVEMVTGKP W+L  +S++ +LL +I  G ESP IP  L  EGKDF+ KC
Sbjct: 181 PADIWALGCAVVEMVTGKPAWNLEKDSNMWSLLLQIGAGEESPLIPEELSKEGKDFVEKC 240

Query: 241 FVKNPNERWTAQMLLDHPFVSGLKDERPSVSPKCPFEFPDWQSNSCSDSESVSSFGFSED 300
           FVK+P +RWTA+MLL+HPFV  + +   S SP+  F+F DW S++   S+SV S   SED
Sbjct: 241 FVKDPRKRWTAEMLLNHPFVEEVINVNES-SPRNHFDFNDWVSDA---SDSVPSSPESED 300

Query: 301 CCWASSWSPVSRTLD--EELISNRIP-NWNDSDDWVTVR 334
                  S     +D   +L++++ P +W+ SD W++VR
Sbjct: 301 SNQWDFDSKFCSAVDRLRQLVTDQGPMSWSRSDSWISVR 335

[Close]

BLAST of Spo08276.1 vs. ExPASy Swiss-Prot
Match: MKKA_DICDI (Mitogen-activated protein kinase kinase kinase A OS=Dictyostelium discoideum GN=mkkA PE=1 SV=2)

HSP 1 Score: 194.9 bits (494), Expect = 1.400e-48
Identity = 106/283 (37.46%), Postives = 160/283 (56.54%), Query Frame = 1


		  
Query: 1   MEWVRGEIIGRGSFGTVSLAIPKSNSNKYVVKSAELSNSFS---LKN-------EKEILS 60
           ++W +G+I+GRG +G+V L + K     + VK  E+ +  S   LKN       E E++ 
Sbjct: 168 IKWQKGQILGRGGYGSVYLGLNKDTGELFAVKQLEIVDINSDPKLKNMILSFSKEIEVMR 227

Query: 61  ELNHPNIVRYLGDSLSLEKGTQFYNLFLEYATKGSIADQLKVSGGRFEFSDVKRYTKSVL 120
            L H NIVRYLG SL       F ++FLEY   GSI+  L   G  F  + +K YTK +L
Sbjct: 228 SLRHDNIVRYLGTSLD----QSFLSVFLEYIPGGSISSLLGKFGA-FSENVIKVYTKQIL 287

Query: 121 KGLCHIHESGFVHCDIKLQNVLLFQDGTAKIADFGLARRVSDEFGE--QLRGTPLYMSPE 180
           +GL  +H +  +H DIK  N+L+   G  K++DFG ++  S    +   ++GTP +M+PE
Sbjct: 288 QGLSFLHANSIIHRDIKGANILIDTKGIVKLSDFGCSKSFSGIVSQFKSMQGTPYWMAPE 347

Query: 181 IVSGEKVESPADVWALGCAVVEMVTGKPVWDLLPESDVSALLYRIAVGGESPKIPGNLCS 240
           ++        +D+W+LGC +VEM T +P W  + E  ++A++Y IA     P IP ++  
Sbjct: 348 VIKQTGHGRSSDIWSLGCVIVEMATAQPPWSNITE--LAAVMYHIASSNSIPNIPSHMSQ 407

Query: 241 EGKDFLGKCFVKNPNERWTAQMLLDHPFVSGLKD--ERPSVSP 270
           E  DFL  CF ++P ER  A  LL HPF+  L D  + P++SP
Sbjct: 408 EAFDFLNLCFKRDPKERPDANQLLKHPFIMNLDDNIQLPTISP 443

[Close]

BLAST of Spo08276.1 vs. ExPASy Swiss-Prot
Match: BCK1_YEAST (Serine/threonine-protein kinase BCK1/SLK1/SSP31 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=BCK1 PE=1 SV=1)

HSP 1 Score: 184.1 bits (466), Expect = 2.500e-45
Identity = 104/273 (38.10%), Postives = 153/273 (56.04%), Query Frame = 1


		  
Query: 3    WVRGEIIGRGSFGTVSLAIPKSNSNKYVVKSAE-----------LSNSFSLKNEKEILSE 62
            W++GE+IG+GSFG V L +  +      VK  E           LS   +L++E   L +
Sbjct: 1175 WMKGEMIGKGSFGAVYLCLNVTTGEMMAVKQVEVPKYSSQNEAILSTVEALRSEVSTLKD 1234

Query: 63   LNHPNIVRYLGDSLSLEKGTQFYNLFLEYATKGSIADQLKVSGGRFEFSDVKRYTKSVLK 122
            L+H NIV+YLG     E     Y+LFLEY   GS+   +++ G RF+   +K  T  VLK
Sbjct: 1235 LDHLNIVQYLG----FENKNNIYSLFLEYVAGGSVGSLIRMYG-RFDEPLIKHLTTQVLK 1294

Query: 123  GLCHIHESGFVHCDIKLQNVLLFQDGTAKIADFGLARRVSDEFGEQ---LRGTPLYMSPE 182
            GL ++H  G +H D+K  N+LL QDG  KI+DFG++R+  D +      +RGT  +M+PE
Sbjct: 1295 GLAYLHSKGILHRDMKADNLLLDQDGICKISDFGISRKSKDIYSNSDMTMRGTVFWMAPE 1354

Query: 183  IVSGEK-VESPADVWALGCAVVEMVTGKPVWDLLPESDVSALLYRIAVGGESPKIPGN-- 242
            +V  ++   +  D+W+LGC V+EM  GK  W  L   +V A +++I     +P IP +  
Sbjct: 1355 MVDTKQGYSAKVDIWSLGCIVLEMFAGKRPWSNL---EVVAAMFKIGKSKSAPPIPEDTL 1414

Query: 243  --LCSEGKDFLGKCFVKNPNERWTAQMLLDHPF 257
              +   G++FL  CF  NP +R TA  LL HPF
Sbjct: 1415 PLISQIGRNFLDACFEINPEKRPTANELLSHPF 1439

[Close]

BLAST of Spo08276.1 vs. ExPASy Swiss-Prot
Match: M3K2_ARATH (Mitogen-activated protein kinase kinase kinase 2 OS=Arabidopsis thaliana GN=ANP2 PE=2 SV=1)

HSP 1 Score: 182.6 bits (462), Expect = 7.200e-45
Identity = 104/299 (34.78%), Postives = 161/299 (53.85%), Query Frame = 1


		  
Query: 1   MEWVRGEIIGRGSFGTVSLAIPKSNSNKYVVKSAELSNSFS-----------LKNEKEIL 60
           + W +G++IGRG+FGTV + +   +     VK   ++++ +           L+ E ++L
Sbjct: 66  IRWRKGQLIGRGAFGTVYMGMNLDSGELLAVKQVLITSNCASKEKTQAHIQELEEEVKLL 125

Query: 61  SELNHPNIVRYLGDSLSLEKGTQFYNLFLEYATKGSIADQLKVSGGRFEFSDVKRYTKSV 120
             L+HPNIVRYLG      +  +  N+ LE+   GSI+  L+  G  F  S V+ YT  +
Sbjct: 126 KNLSHPNIVRYLGTV----REDETLNILLEFVPGGSISSLLEKFGA-FPESVVRTYTNQL 185

Query: 121 LKGLCHIHESGFVHCDIKLQNVLLFQDGTAKIADFGLARRVSD----EFGEQLRGTPLYM 180
           L GL ++H    +H DIK  N+L+   G  K+ADFG +++V++       + ++GTP +M
Sbjct: 186 LLGLEYLHNHAIMHRDIKGANILVDNQGCIKLADFGASKQVAELATISGAKSMKGTPYWM 245

Query: 181 SPEIVSGEKVESPADVWALGCAVVEMVTGKPVWDLLPESDVSALLYRIAVGGESPKIPGN 240
           +PE++        AD+W++GC V+EMVTGK  W    +    A ++ I      P IP N
Sbjct: 246 APEVILQTGHSFSADIWSVGCTVIEMVTGKAPWS--QQYKEIAAIFHIGTTKSHPPIPDN 305

Query: 241 LCSEGKDFLGKCFVKNPNERWTAQMLLDHPFVSGLKDERPSVSPKCPFEFPDWQSNSCS 285
           + S+  DFL KC  + PN R TA  LL HPFV+G + E  S       +   +  NSCS
Sbjct: 306 ISSDANDFLLKCLQQEPNLRPTASELLKHPFVTGKQKESASK------DLTSFMDNSCS 351

[Close]

BLAST of Spo08276.1 vs. ExPASy Swiss-Prot
Match: ANP1_ARATH (Mitogen-activated protein kinase kinase kinase ANP1 OS=Arabidopsis thaliana GN=ANP1 PE=1 SV=2)

HSP 1 Score: 178.3 bits (451), Expect = 1.400e-43
Identity = 110/320 (34.38%), Postives = 170/320 (53.12%), Query Frame = 1


		  
Query: 1   MEWVRGEIIGRGSFGTVSLAIPKSNSNKYVVKSAELSNSFS-----------LKNEKEIL 60
           + W +G++IGRG+FGTV + +   +     VK   ++ +F+           L+ E ++L
Sbjct: 67  ISWRKGQLIGRGAFGTVYMGMNLDSGELLAVKQVLIAANFASKEKTQAHIQELEEEVKLL 126

Query: 61  SELNHPNIVRYLGDSLSLEKGTQFYNLFLEYATKGSIADQLKVSGGRFEFSDVKRYTKSV 120
             L+HPNIVRYLG      +     N+ LE+   GSI+  L+   G F  S V+ YT+ +
Sbjct: 127 KNLSHPNIVRYLGTV----REDDTLNILLEFVPGGSISSLLE-KFGPFPESVVRTYTRQL 186

Query: 121 LKGLCHIHESGFVHCDIKLQNVLLFQDGTAKIADFGLARRVSD----EFGEQLRGTPLYM 180
           L GL ++H    +H DIK  N+L+   G  K+ADFG +++V++       + ++GTP +M
Sbjct: 187 LLGLEYLHNHAIMHRDIKGANILVDNKGCIKLADFGASKQVAELATMTGAKSMKGTPYWM 246

Query: 181 SPEIVSGEKVESPADVWALGCAVVEMVTGKPVWDLLPESDVSALLYRIAVGGESPKIPGN 240
           +PE++        AD+W++GC V+EMVTGK  W      +V+A+ + I      P IP  
Sbjct: 247 APEVILQTGHSFSADIWSVGCTVIEMVTGKAPWS-QQYKEVAAIFF-IGTTKSHPPIPDT 306

Query: 241 LCSEGKDFLGKCFVKNPNERWTAQMLLDHPFVSGLKDERPS---------VSPKCPFEFP 294
           L S+ KDFL KC  + PN R TA  LL HPFV G   E  S         +S   P +  
Sbjct: 307 LSSDAKDFLLKCLQEVPNLRPTASELLKHPFVMGKHKESASTDLGSVLNNLSTPLPLQIN 366

[Close]

BLAST of Spo08276.1 vs. ExPASy Swiss-Prot
Match: NPK1_TOBAC (Mitogen-activated protein kinase kinase kinase NPK1 OS=Nicotiana tabacum GN=NPK1 PE=1 SV=1)

HSP 1 Score: 176.4 bits (446), Expect = 5.200e-43
Identity = 99/278 (35.61%), Postives = 153/278 (55.04%), Query Frame = 1


		  
Query: 1   MEWVRGEIIGRGSFGTVSLAIPKSNSNKYVVKSAELSNSFS-----------LKNEKEIL 60
           + W +GE+IG G+FG V + +   +     +K   ++ + +           L+ E  +L
Sbjct: 78  IRWRKGEMIGCGAFGRVYMGMNVDSGELLAIKEVSIAMNGASRERAQAHVRELEEEVNLL 137

Query: 61  SELNHPNIVRYLGDSLSLEKGTQFYNLFLEYATKGSIADQLKVSGGRFEFSDVKRYTKSV 120
             L+HPNIVRYLG +   E G+   N+ LE+   GSI+  L   G  F  S ++ YTK +
Sbjct: 138 KNLSHPNIVRYLGTAR--EAGS--LNILLEFVPGGSISSLLGKFGS-FPESVIRMYTKQL 197

Query: 121 LKGLCHIHESGFVHCDIKLQNVLLFQDGTAKIADFGLARRVSD----EFGEQLRGTPLYM 180
           L GL ++H++G +H DIK  N+L+   G  K+ADFG +++V +       + ++GTP +M
Sbjct: 198 LLGLEYLHKNGIMHRDIKGANILVDNKGCIKLADFGASKKVVELATMTGAKSMKGTPYWM 257

Query: 181 SPEIVSGEKVESPADVWALGCAVVEMVTGKPVWDLLPESDVSALLYRIAVGGESPKIPGN 240
           +PE++        AD+W++GC ++EM TGKP W    +    A L+ I      P IP +
Sbjct: 258 APEVILQTGHSFSADIWSVGCTIIEMATGKPPWS--QQYQEVAALFHIGTTKSHPPIPEH 317

Query: 241 LCSEGKDFLGKCFVKNPNERWTAQMLLDHPFVSGLKDE 264
           L +E KDFL KC  K P+ R +A  LL HPFV+    E
Sbjct: 318 LSAESKDFLLKCLQKEPHLRHSASNLLQHPFVTAEHQE 348

[Close]

BLAST of Spo08276.1 vs. TAIR (Arabidopsis)
Match: AT4G36950.1 (mitogen-activated protein kinase kinase kinase 21)

HSP 1 Score: 325.9 bits (834), Expect = 3.000e-89
Identity = 175/346 (50.58%), Postives = 231/346 (66.76%), Query Frame = 1


		  
Query: 1   MEWVRGEIIGRGSFGTVSLAIPKSNSNK-----YVVKSAELSNSFSLKNEKEILSELNH- 60
           MEW+R E IG GSF TVSLA    +S+K       VKS+ +  S +L+NE+++L +L   
Sbjct: 1   MEWIRRETIGHGSFSTVSLATTSGSSSKAFPSLMAVKSSGVVCSAALRNERDVLDDLGDC 60

Query: 61  PNIVRYLGDSLSLEKGTQFYNLFLEYATKGSIADQLKVSGGRFEFSDVKRYTKSVLKGLC 120
             IVR  G+  ++E G + YNLFLEYA+ GS+AD++K SG      +V+R+T+S++KGLC
Sbjct: 61  SEIVRCFGEGRTVENGEEIYNLFLEYASGGSLADRIKSSGEALPEFEVRRFTRSIVKGLC 120

Query: 121 HIHESGFVHCDIKLQNVLLFQDGTAKIADFGLARRVSDEFGEQLRGTPLYMSPEIVSGEK 180
           HIH +GF HCDIKL+NVL+F DG  KI+DFGLA+R S E   ++RGTPLYM+PE V+  +
Sbjct: 121 HIHGNGFTHCDIKLENVLVFGDGDVKISDFGLAKRRSGEVCVEIRGTPLYMAPESVNHGE 180

Query: 181 VESPADVWALGCAVVEMVTGKPVWDLLP--ESDVSALLYRIAVGGESPKIPGNLCSEGKD 240
            ESPAD+WALGC+VVEM +GK  W L     ++V +LL RI  G E P+IP  L  EGKD
Sbjct: 181 FESPADIWALGCSVVEMSSGKTAWCLEDGVMNNVMSLLVRIGSGDEVPRIPVELSEEGKD 240

Query: 241 FLGKCFVKNPNERWTAQMLLDHPFV-----SGLKDERPSVSPKCPFEFPDWQSNSCSDSE 300
           F+ KCFVKN  ERWTA+MLLDHPF+     SG +DE  SVSP+ PF+FP W S     ++
Sbjct: 241 FVSKCFVKNAAERWTAEMLLDHPFLAVDDESGEEDEACSVSPRNPFDFPGWNSVQSPVND 300

Query: 301 SVSSFGFSEDCCWASSWSPVSRTLDEELISNRIPNWNDSDDWVTVR 334
           SV  FG       +   SP  R     L+S ++P+W+ S DWV VR
Sbjct: 301 SV-MFG-------SLVGSPEERI--SGLVSEKVPDWSVSCDWVNVR 336

[Close]

BLAST of Spo08276.1 vs. TAIR (Arabidopsis)
Match: AT5G67080.1 (mitogen-activated protein kinase kinase kinase 19)

HSP 1 Score: 310.8 bits (795), Expect = 9.900e-85
Identity = 170/361 (47.09%), Postives = 222/361 (61.50%), Query Frame = 1


		  
Query: 1   MEWVRGEIIGRGSFGTVSLAIPKSNSNK-----YVVKSAELSNSFSLKNEKEILSELNHP 60
           MEW+RGE IG G+F TVSLA   +N +        VKSA+   + SL NEK +L  L   
Sbjct: 1   MEWIRGETIGYGTFSTVSLATRSNNDSGEFPPLMAVKSADSYGAASLANEKSVLDNLGDD 60

Query: 61  --NIVRYLGDSLSLEKGTQFYNLFLEYATKGSIADQLK-VSGGRFEFSDVKRYTKSVLKG 120
              IVR  G+  ++E G + +NLFLEYA++GS+   LK ++G     S V+R+T SVL+G
Sbjct: 61  CNEIVRCFGEDRTVENGEEMHNLFLEYASRGSLESYLKKLAGEGVPESTVRRHTGSVLRG 120

Query: 121 LCHIHESGFVHCDIKLQNVLLFQDGTAKIADFGLARRVSD----EFGEQLRGTPLYMSPE 180
           L HIH +GF HCD+KL N+LLF DG  KIADFGLA+R+ D     +G Q+RGTPLYM+PE
Sbjct: 121 LRHIHANGFAHCDLKLGNILLFGDGAVKIADFGLAKRIGDLTALNYGVQIRGTPLYMAPE 180

Query: 181 IVSGEKVESPADVWALGCAVVEMVTGKPVWDLLPESDVSALLYRIAVGGESPKIPGNLCS 240
            V+  +  S  DVWALGC VVEM +GK  W L   S+  +LL RI VG E P IP  L  
Sbjct: 181 SVNDNEYGSEGDVWALGCVVVEMFSGKTAWSLKEGSNFMSLLLRIGVGDEVPMIPEELSE 240

Query: 241 EGKDFLGKCFVKNPNERWTAQMLLDHPFVS----------------GLKDERPSVSPKCP 300
           +G+DFL KCFVK+P +RWTA+MLL+HPFV+                 +K E  S SP+CP
Sbjct: 241 QGRDFLSKCFVKDPKKRWTAEMLLNHPFVTVDVDHDVLVKEEDFVVNMKTEDVSTSPRCP 300

Query: 301 FEFPDWQSNSCSDSESVSSFGFSEDCCWASSWSPVSRTLDEELISNRIPNWNDSDDWVTV 334
           FEFPDW S S S S+++               SP  R     L+++ IP+W+ ++ WVTV
Sbjct: 301 FEFPDWVSVS-SGSQTID--------------SPDERV--ASLVTDMIPDWSVTNSWVTV 344

[Close]

BLAST of Spo08276.1 vs. TAIR (Arabidopsis)
Match: AT3G50310.1 (mitogen-activated protein kinase kinase kinase 20)

HSP 1 Score: 306.2 bits (783), Expect = 2.400e-83
Identity = 171/358 (47.77%), Postives = 214/358 (59.78%), Query Frame = 1


		  
Query: 1   MEWVRGEIIGRGSFGTVSLAIPKSNSNKY----VVKSAELSNSFSLKNEKEILSELNH-P 60
           MEWVRGE IG G+F TVS A    NS  +     VKS +   + SL NEK +L  L   P
Sbjct: 1   MEWVRGETIGFGTFSTVSTATKSRNSGDFPALIAVKSTDAYGAASLSNEKSVLDSLGDCP 60

Query: 61  NIVRYLGDSLSLEKGTQFYNLFLEYATKGSIADQLKVSGGR-FEFSDVKRYTKSVLKGLC 120
            I+R  G+  ++E G + +NL LEYA++GS+A  +K  GG     S V+R+T SVL+GL 
Sbjct: 61  EIIRCYGEDSTVENGEEMHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSVLRGLR 120

Query: 121 HIHESGFVHCDIKLQNVLLFQDGTAKIADFGLARRVSDEF-----GEQLRGTPLYMSPEI 180
           HIH  GF HCDIKL N+LLF DG+ KIADFGLA RV  +        ++RGTPLYM+PE 
Sbjct: 121 HIHAKGFAHCDIKLANILLFNDGSVKIADFGLAMRVDGDLTALRKSVEIRGTPLYMAPEC 180

Query: 181 VSGEKVESPADVWALGCAVVEMVTGKPVWDLLPESDVSALLYRIAVGGESPKIPGNLCSE 240
           V+  +  S ADVWALGCAVVEM +GK  W +   S   +LL RI VG E PKIP  L  E
Sbjct: 181 VNDNEYGSAADVWALGCAVVEMFSGKTAWSVKEGSHFMSLLIRIGVGDELPKIPEMLSEE 240

Query: 241 GKDFLGKCFVKNPNERWTAQMLLDHPFVS-------------GLKDE-RPSVSPKCPFEF 300
           GKDFL KCFVK+P +RWTA+MLL+H FV+              +KDE +  +SPKCPFEF
Sbjct: 241 GKDFLSKCFVKDPAKRWTAEMLLNHSFVTIDLEDDHRENFVVKVKDEDKVLMSPKCPFEF 300

Query: 301 PDWQSNSCSDSESVSSFGFSEDCCWASSWSPVSRTLDEELISNRIPNWNDSDDWVTVR 334
            DW S +   + S                SPV R     L+S  IP+W+    W+TVR
Sbjct: 301 DDWDSFTLDSNPSFD--------------SPVERL--GSLVSGSIPDWSVGGSWLTVR 342

[Close]

BLAST of Spo08276.1 vs. TAIR (Arabidopsis)
Match: AT5G55090.1 (mitogen-activated protein kinase kinase kinase 15)

HSP 1 Score: 246.5 bits (628), Expect = 2.300e-65
Identity = 139/346 (40.17%), Postives = 198/346 (57.23%), Query Frame = 1


		  
Query: 3   WVRGEIIGRGSFGTVSLAIPKSNSNKYVVKSAELSNSFSLKNEKEILSELNHPNIVRYLG 62
           W+RG IIGRGS  TVSL I  S  + + VKSAE S+S  L+ E+ ILS+L+ P IV+Y+G
Sbjct: 6   WIRGPIIGRGSTATVSLGITNSG-DFFAVKSAEFSSSAFLQREQSILSKLSSPYIVKYIG 65

Query: 63  DSLSLEKGTQFYNLFLEYATKGSIADQLKVSGGRFEFSDVKRYTKSVLKGLCHIHESGFV 122
            +++ E     YNL +EY + GS+ D +K SGG+     ++ YT+ +LKGL ++H+ G V
Sbjct: 66  SNVTKENDKLMYNLLMEYVSGGSLHDLIKNSGGKLPEPLIRSYTRQILKGLMYLHDQGIV 125

Query: 123 HCDIKLQNVLLFQDGTAKIADFGLARRVSDEFGEQLRGTPLYMSPEIVSGEKVESPADVW 182
           HCD+K QNV++  +  AKI D G A+ V +    +  GTP +MSPE+  GE+   PADVW
Sbjct: 126 HCDVKSQNVMIGGE-IAKIVDLGCAKTVEENENLEFSGTPAFMSPEVARGEEQSFPADVW 185

Query: 183 ALGCAVVEMVTGKPVWDLLPESDVSALLYRIAVGGESPKIPGNLCSEGKDFLGKCFVKNP 242
           ALGC V+EM TG   W  L  +DV A +Y+I   GESP IP  L  +G+DFL KC  K+P
Sbjct: 186 ALGCTVIEMATGSSPWPEL--NDVVAAIYKIGFTGESPVIPVWLSEKGQDFLRKCLRKDP 245

Query: 243 NERWTAQMLLDHPFVSGLKDERP-------SVSPKCPFEFPDWQSNSCSDSESVSS---- 302
            +RWT + LL HPF+    ++         S SP    +   W     S S  +      
Sbjct: 246 KQRWTVEELLQHPFLDEEDNDSDQTGNCLNSSSPSTVLDQRFWDLCETSRSRFIKEDHED 305

Query: 303 -FGFSEDCCWASSWSP---VSRTLDEELISNRIPNWNDSDDWVTVR 334
            F  S +  W     P   + +   +E  S+  P+W +++ W+ VR
Sbjct: 306 PFANSTNFLWDDDSLPGDRIKKLAGDE--SSGEPDW-ETNGWIEVR 344

[Close]

BLAST of Spo08276.1 vs. TAIR (Arabidopsis)
Match: AT2G32510.1 (mitogen-activated protein kinase kinase kinase 17)

HSP 1 Score: 244.2 bits (622), Expect = 1.100e-64
Identity = 119/271 (43.91%), Postives = 163/271 (60.15%), Query Frame = 1


		  
Query: 1   MEWVRGEIIGRGSFGTVSLAIPKSNSNKYVVKSAELSNSFSLKNEKEILSELNHPNIVRY 60
           MEW RG I+GRGS  TV  A   ++     VKS+E+  S  L+ E +ILS L+ P ++ Y
Sbjct: 1   MEWTRGRILGRGSTATVYAAAGHNSDEILAVKSSEVHRSEFLQREAKILSSLSSPYVIGY 60

Query: 61  LGDSLSLEK-GTQFYNLFLEYATKGSIADQLKVSGGRFEFSDVKRYTKSVLKGLCHIHES 120
            G     E  G   YNL +EYA  G++ D     GGR + + V +YT+ +LKGL +IH  
Sbjct: 61  RGSETKRESNGVVMYNLLMEYAPYGTLTDAAAKDGGRVDETRVVKYTRDILKGLEYIHSK 120

Query: 121 GFVHCDIKLQNVLLFQDGTAKIADFGLARRVSDEFGEQLRGTPLYMSPEIVSGEKVESPA 180
           G VHCD+K  NV++ + G AKIADFG A+RV   F   + GTP +M+PE+  GEK    +
Sbjct: 121 GIVHCDVKGSNVVISEKGEAKIADFGCAKRVDPVFESPVMGTPAFMAPEVARGEKQGKES 180

Query: 181 DVWALGCAVVEMVTGKPVWDLL-PESDVSALLYRIAVGGESPKIPGNLCSEGKDFLGKCF 240
           D+WA+GC ++EMVTG P W       D  ++LYR+    E+P++P  L  E KDFL KC 
Sbjct: 181 DIWAVGCTMIEMVTGSPPWTKADSREDPVSVLYRVGYSSETPELPCLLAEEAKDFLEKCL 240

Query: 241 VKNPNERWTAQMLLDHPFVSGLKDERPSVSP 270
            +  NERWTA  LL+HPF++   D  P + P
Sbjct: 241 KREANERWTATQLLNHPFLTTKPDIEPVLVP 271

The following BLAST results are available for this feature:

BLAST of Spo08276.1 vs. NCBI nr
BLAST of Spo08276.1 vs. UniProtKB/TrEMBL
BLAST of Spo08276.1 vs. ExPASy Swiss-Prot
BLAST of Spo08276.1 vs. TAIR (Arabidopsis)

BLAST of Spo08276.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902176008\|gb\|KNA08706.1\|	2.2e-195	100.	hypothetical protein SOVF_1602...	[more]
gi\|731375935\|ref\|XP_010667409.1\|	8.6e-147	73.9	PREDICTED: mitogen-activated p...	[more]
gi\|1009144451\|ref\|XP_015889808.1\|	5.3e-96	52.5	PREDICTED: mitogen-activated p...	[more]
gi\|823139394\|ref\|XP_012469556.1\|	9.9e-95	50.8	PREDICTED: mitogen-activated p...	[more]
gi\|590704879\|ref\|XP_007047281.1\|	2.9e-94	50.6	Mitogen-activated protein kina...	[more]

BLAST of Spo08276.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9QQB3_SPIOL	1.5e-195	100.	Uncharacterized protein OS=Spi...	[more]
A0A0J8B321_BETVU	6.0e-147	73.9	Uncharacterized protein OS=Bet...	[more]
A0A0D2QEQ1_GOSRA	6.9e-95	50.8	Uncharacterized protein OS=Gos...	[more]
A0A061DN83_THECC	2.0e-94	50.6	Mitogen-activated protein kina...	[more]
A0A072TH41_MEDTR	5.5e-92	52.2	MAP3K-like kinase OS=Medicago ...	[more]

BLAST of Spo08276.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
MKKA_DICDI	1.4e-48	37.4	Mitogen-activated protein kina...	[more]
BCK1_YEAST	2.5e-45	38.1	Serine/threonine-protein kinas...	[more]
M3K2_ARATH	7.2e-45	34.7	Mitogen-activated protein kina...	[more]
ANP1_ARATH	1.4e-43	34.3	Mitogen-activated protein kina...	[more]
NPK1_TOBAC	5.2e-43	35.6	Mitogen-activated protein kina...	[more]

BLAST of Spo08276.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 5


Match Name	E-value	Identity	Description
AT4G36950.1	3.0e-89	50.5	mitogen-activated protein kina...	[more]
AT5G67080.1	9.9e-85	47.0	mitogen-activated protein kina...	[more]
AT3G50310.1	2.4e-83	47.7	mitogen-activated protein kina...	[more]
AT5G55090.1	2.3e-65	40.1	mitogen-activated protein kina...	[more]
AT2G32510.1	1.1e-64	43.9	mitogen-activated protein kina...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000719	Protein kinase domain	PFAM	PF00069	Pkinase	coord: 5..257 score: 3.5
IPR000719	Protein kinase domain	SMART	SM00220	serkin_6	coord: 3..257 score: 2.8
IPR000719	Protein kinase domain	PROFILE	PS50011	PROTEIN_KINASE_DOM	coord: 3..257 score: 41
IPR008271	Serine/threonine-protein kinase, active site	PROSITE	PS00108	PROTEIN_KINASE_ST	coord: 121..133 scor
IPR011009	Protein kinase-like domain	unknown	SSF56112	Protein kinase-like (PK-like)	coord: 3..283 score: 7.69
IPR017441	Protein kinase, ATP binding site	PROSITE	PS00107	PROTEIN_KINASE_ATP	coord: 9..32 scor
None	No IPR available	GENE3D	1.10.510.10		coord: 53..288 score: 5.6
None	No IPR available	GENE3D	3.30.200.20		coord: 3..52 score: 1.8
None	No IPR available	PANTHER	PTHR24361	MITOGEN-ACTIVATED KINASE KINASE KINASE	coord: 1..333 score: 7.7E
None	No IPR available	PANTHER	PTHR24361:SF334	MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 19-RELATED	coord: 1..333 score: 7.7E

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004672	protein kinase activity