Spo08328.1 (mRNA) | SpinachBase

Overview


Name	Spo08328.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	(Ascorbate peroxidase) (1.11.1.11)
Location	SpoScf_01273 : 153370 .. 157514 (-)
Sequence length	1469

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CAGTAGTATTTTTTTGGGTACATTCATATAATATTTTAAAATATTCCCCCCCTTTGCTTATTTATAGAGATGAATGAGCAAAAATAAGAATTCAAAACAAATCAAAACGAGTAATAATACATTTGTGTTGATCAAGTTCCAAAATGGGAAGAGTACCAATTGTGAATGAAAACTATCGGAGGGTAATTGAGGCAGCTCGTCGAGACCTCCACCGTTCTCTCGTCCAGGACAACAACAACTCTGCTCCTATCCTCCTCCGCCTCTCGTAACTTCTCTCTCTTTATTTAACATACCACGATATTTCTTTATTTATCTCTTAAACTGACTAATTTTACAAGTCTTTGGTAGTTTAGTACCATATTATTGACAAAGTGTGTTGGACTTATTTCAGACAAAATAAGTTCATGAAAAAAAAAAATCATATATTATGTTTATTATATACAAAAATAACTTCTTCTTTTTTTTCAGTTTAGATAAACCAGATAATTTCACATAAATTTAGATAATATAAATTCACTCAAAATAAGTCGAATATACCAAAAAAAATATCGAATATAATGGAGTCTAACTGTACTAATGAAATCTTAACCTTTTGGTTAAGACCGAGGGCATGTGTACAAATCTACAATAAATGACAGGTTCAAATTTTTCCAAACCCATTTGTAATTTGTATTGCCGTTATACCTCATTCGTACCAAAAAATAATATTATTGACTGCATGCTACTCCGTATTTGTATTCCGACTCCGTAGTATTTTACAATATCAATTTAATTTTCACAGATACTAACCCAACTATTTAAACGTATGTGACAAAATCGATGTGTTTTTTATTTAAGGAAACGGCTCCGGCCACTAGCTTTCATTTTTTCATGATTAAGTAAATAAAAATCAGATGCAGTTACATCCTCTACTAAGGTTGGACAATCCCCTATCCACACTTGTGTAGGGAACCCGAGTGGGCTATTATATTTCCAAAGAGTAACTTGGTTTTGGTGTTGTAGATTTCACGATGCAGTGGACTATGATGCAGCAACAAAACGAGGTGGTGCAAATGGTTCAGTTAGGTTAGCTCAGGAGTTGAACCGCACCCCAAACAAGGGCATAGAAACTGCTGTTAGATTTTGTGGTGAGCTTATCAATTTTACTACATTTTTTGATGGAAAATTCTCTACTTATTAAGCACAAATACATTTTCTGGGTAATAATTATTTCGTGCAAAGTCTACACTAGCTATTTGGTACTAAACGTCTATCTATTTCACCAGCAAGTCTACAACTACTATTTGGAGGATAACTTATTGTTTTTTTTTATTTTCCCCCTTTGTGCAGAGCCGATCAAGCGTAGACACCCTGATATTACATATGCTGACCTTTATCAGGTAAACTATGCATCTTTAAGGATGCATCTAAGCTAGGGAGAAGCCATATTTTGATTGTTAAGACGAGTCTACCAAGTGGGCTTAGAGGGGCAGACGAGTAAAAAACTAGGAAATTTGTTGAAATATTATCTTTAAGTGTAGAGGTTTTGTGAATTGCTATATTTTAAAAAACAATATTAAATTTTGCCGTTTAACTATCTTATAAGTTTAGTTATTTGACTAACAATGCCATTTGACGTATGTCGTTAATGTTTTTTGTCTGTGAACAAATCTATTTGACAAATCTATTTAATATACAAATGAACAAATATATTTAAAGAAATAGAAGAAATACTCGATGAAAAATATTACTCCCTTTGAATCTAAATGTTTTTCCATTTAGAAACTTGACACTATTCACAATAGAAGATAATCTTATGAATTACGTTCAATATATAAGAAAAAACATAGTCATGTGGGATCTTGTTTGATTCTCTCCACGAGTACTTTAACAATATGAAAATTTAATAATTTTTAATAATACGTTACTAGAGATATTAACGATGTAAAATGTGCATTGACATGTGTGTCAAAGAAAAATGGGAAAAACATTTAGATTCGGAGGGAGTATCGGAAAACAATTTACGAAATCACCAAACTTGAAGGTATTGTTTCACAAAATTATTTTAATTTTCCGGGATAACTCGGCCTTTTAAATTCAACTGCAGTCCAAAAAGAGATACAATAAGATTTACTTTTTGAAACTAGAGATAAAATAAGGGATATTAAATTATAAAAAGTTGATATTTTGAGAACACGCATTGAAACAAATCTAACAAAATCTCACATTATTATGTTTTATCTAACATATAAATCATCAACAATTGTTAAAGTAAAATATGTGAATAGTGTAAAAAGTTTAAACGTTGTGAGTATTTCTAAACGAGGAATTACGAGTAGGTTTTTTCCAACACAAAATATATTTTGAACTTTCCAACAAAAAAAATTGATTTTTGTTGTTCATTACTGGTTTTCTCAGCTAGCTGGAATTGTTGCAGTAGAAGTAACCGGAGGTCCTGCTATTGATTTTGTTCCAGGTCGAGCTGTGAGTATTGCTAACTTTGTAATGCTCATGCACAATATTTTAGGCTTTTTGTGACAAATATATAAACAGCATATATATACGGAGTATGTGCGATGTGCCTAATAATTGTCATCTTTCAAATAGGATGCTGATGTTGCAGACCAAGACAATATCCCAAACCCTAGAAGAGGTATCCACTAGTTAATATTCTTACACAATAGGATAATATTAGCAATATCTAACCCATGTGATTTTATGGGTTTTAGGAGCGGATCATCTGAGAACCGTATTTTATCGAATGGGCCTCAATGACAAGGATATCGTTGTACTTTCTGGGGCCCACGCACTGGTGAGATTGACTTTGCTTTGAATTTTAATTTGGTTGCACTGAAGCTGTGCAATAATAAAAAGTAAACTAGTGGAAGTATAAATTTAAAAAAAAATACTTATAGGTTTGCGTTTTGATATGCATATATGTTTGGAATGTGAAGGGTGGAGCACACAAGGACAGATCAGGGTTTGACGGCGATTTCACTCGAAACCCTTTGACGTTTGATAACTCCTACTTTGTGTAAGAATATTAATTAGATATTTGTATGTAAGAATAAGTTGTGTATAATATGGTTCACAGTAAGATCGATAATATAACGAGTCTAAAACAAAGTTTCATATCTATTGTAAATTTGTAATAGCTATCTTATCGTGTACTGTGTGTATTTGTATGATCAGAGAGCTACTGAGGGGTGATACTCCAGGGTTGGTGAAATTTCCAACAGATAAGGCTCTCTTAACTGACCCTCGTTTCCGTCCCTTTGTTGATCTCTACGCTAGGGTATGTCCTCTTACTTAATATATATATGCACAAATTAATGACCTTAATTTAATTAAACTATATTAGCTAGCTTACCAAGCTTACTTGGTGGAGATGGTGACATATGGTACTTACCTACCTTGTGTCTTGAAAGTCACAATTAACAAGACAATATATACTTGATAATAACTAACAAGTGTAGTAGTAATGTTTAAACTAACAAGCAGCAAATGACGTACAGGATCAAAGAGCATTCTTCAGAGATTATGCAGAATCACACAAGAAGATGTCCCTGCTAGGGCTTAATCATCCGGAATCCAACCTATATGAAAGTAACAGCTGCAGCGACGAGGTTGAGTGTGGTGCTCAATCCTACGTTGTCCAAAACGGAGGCTGTCCAATGCAATACGGATATGCTCGACCCTATGCAGTTGGAAATGGTTGCTGCCCAGGCGGCTACTGATACATATAATATGCCCATATATACGGCTGTTAACTGTAACAGTCTACGTGACTGAGTGAGTACGTGATGGGAATTAATATCTTTGAGTTCATGTGATGGCCTCAAGAATAATTAAGTTGTAACGTTTTACTTTGTTATATATACTCGTTTATTATGTAATTAAAGTTGTAGTAGTAACGGGGAAACTGTACGTGACAGGAACTTGCTTGGAGAAAATCAGCCTTCCTGCCCTCCCTGCATGCTATAAATAAATAACAAACACTAATATATTTTTAGTTTTATATTTGCTTCACTTTGTTTTTCCATGCATCTTTTTGCATGCCTAATTGATATCGTTAATCATGTAAAGTCTTTTATTTTGATTATTAGTTAAGCTTCGATCTTTAGTAGAAAATTTGTTATAGTCCTATAGATGGGTATACGTACCTC

mRNA sequence

CAGTAGTATTTTTTTGGGTACATTCATATAATATTTTAAAATATTCCCCCCCTTTGCTTATTTATAGAGATGAATGAGCAAAAATAAGAATTCAAAACAAATCAAAACGAGTAATAATACATTTGTGTTGATCAAGTTCCAAAATGGGAAGAGTACCAATTGTGAATGAAAACTATCGGAGGGTAATTGAGGCAGCTCGTCGAGACCTCCACCGTTCTCTCGTCCAGGACAACAACAACTCTGCTCCTATCCTCCTCCGCCTCTCATTTCACGATGCAGTGGACTATGATGCAGCAACAAAACGAGGTGGTGCAAATGGTTCAGTTAGGTTAGCTCAGGAGTTGAACCGCACCCCAAACAAGGGCATAGAAACTGCTGTTAGATTTTGTGAGCCGATCAAGCGTAGACACCCTGATATTACATATGCTGACCTTTATCAGCTAGCTGGAATTGTTGCAGTAGAAGTAACCGGAGGTCCTGCTATTGATTTTGTTCCAGGTCGAGCTGATGCTGATGTTGCAGACCAAGACAATATCCCAAACCCTAGAAGAGGAGCGGATCATCTGAGAACCGTATTTTATCGAATGGGCCTCAATGACAAGGATATCGTTGTACTTTCTGGGGCCCACGCACTGGGTGGAGCACACAAGGACAGATCAGGGTTTGACGGCGATTTCACTCGAAACCCTTTGACGTTTGATAACTCCTACTTTGTAGAGCTACTGAGGGGTGATACTCCAGGGTTGGTGAAATTTCCAACAGATAAGGCTCTCTTAACTGACCCTCGTTTCCGTCCCTTTGTTGATCTCTACGCTAGGGATCAAAGAGCATTCTTCAGAGATTATGCAGAATCACACAAGAAGATGTCCCTGCTAGGGCTTAATCATCCGGAATCCAACCTATATGAAAGTAACAGCTGCAGCGACGAGGTTGAGTGTGGTGCTCAATCCTACGTTGTCCAAAACGGAGGCTGTCCAATGCAATACGGATATGCTCGACCCTATGCAGTTGGAAATGGTTGCTGCCCAGGCGGCTACTGATACATATAATATGCCCATATATACGGCTGTTAACTGTAACAGTCTACGTGACTGAGTGAGTACGTGATGGGAATTAATATCTTTGAGTTCATGTGATGGCCTCAAGAATAATTAAGTTGTAACGTTTTACTTTGTTATATATACTCGTTTATTATGTAATTAAAGTTGTAGTAGTAACGGGGAAACTGTACGTGACAGGAACTTGCTTGGAGAAAATCAGCCTTCCTGCCCTCCCTGCATGCTATAAATAAATAACAAACACTAATATATTTTTAGTTTTATATTTGCTTCACTTTGTTTTTCCATGCATCTTTTTGCATGCCTAATTGATATCGTTAATCATGTAAAGTCTTTTATTTTGATTATTAGTTAAGCTTCGATCTTTAGTAGAAAATTTGTTATAGTCCTATAGATGGGTATACGTACCTC

Coding sequence (CDS)

ATGGGAAGAGTACCAATTGTGAATGAAAACTATCGGAGGGTAATTGAGGCAGCTCGTCGAGACCTCCACCGTTCTCTCGTCCAGGACAACAACAACTCTGCTCCTATCCTCCTCCGCCTCTCATTTCACGATGCAGTGGACTATGATGCAGCAACAAAACGAGGTGGTGCAAATGGTTCAGTTAGGTTAGCTCAGGAGTTGAACCGCACCCCAAACAAGGGCATAGAAACTGCTGTTAGATTTTGTGAGCCGATCAAGCGTAGACACCCTGATATTACATATGCTGACCTTTATCAGCTAGCTGGAATTGTTGCAGTAGAAGTAACCGGAGGTCCTGCTATTGATTTTGTTCCAGGTCGAGCTGATGCTGATGTTGCAGACCAAGACAATATCCCAAACCCTAGAAGAGGAGCGGATCATCTGAGAACCGTATTTTATCGAATGGGCCTCAATGACAAGGATATCGTTGTACTTTCTGGGGCCCACGCACTGGGTGGAGCACACAAGGACAGATCAGGGTTTGACGGCGATTTCACTCGAAACCCTTTGACGTTTGATAACTCCTACTTTGTAGAGCTACTGAGGGGTGATACTCCAGGGTTGGTGAAATTTCCAACAGATAAGGCTCTCTTAACTGACCCTCGTTTCCGTCCCTTTGTTGATCTCTACGCTAGGGATCAAAGAGCATTCTTCAGAGATTATGCAGAATCACACAAGAAGATGTCCCTGCTAGGGCTTAATCATCCGGAATCCAACCTATATGAAAGTAACAGCTGCAGCGACGAGGTTGAGTGTGGTGCTCAATCCTACGTTGTCCAAAACGGAGGCTGTCCAATGCAATACGGATATGCTCGACCCTATGCAGTTGGAAATGGTTGCTGCCCAGGCGGCTACTGA

Protein sequence

MGRVPIVNENYRRVIEAARRDLHRSLVQDNNNSAPILLRLSFHDAVDYDAATKRGGANGSVRLAQELNRTPNKGIETAVRFCEPIKRRHPDITYADLYQLAGIVAVEVTGGPAIDFVPGRADADVADQDNIPNPRRGADHLRTVFYRMGLNDKDIVVLSGAHALGGAHKDRSGFDGDFTRNPLTFDNSYFVELLRGDTPGLVKFPTDKALLTDPRFRPFVDLYARDQRAFFRDYAESHKKMSLLGLNHPESNLYESNSCSDEVECGAQSYVVQNGGCPMQYGYARPYAVGNGCCPGGY

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo08328	Spo08328	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo08328.1	Spo08328.1-protein	polypeptide

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo08328.1.utr5p.1	Spo08328.1.utr5p.1	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo08328.1.CDS.9	Spo08328.1.CDS.9	CDS
Spo08328.1.CDS.8	Spo08328.1.CDS.8	CDS
Spo08328.1.CDS.7	Spo08328.1.CDS.7	CDS
Spo08328.1.CDS.6	Spo08328.1.CDS.6	CDS
Spo08328.1.CDS.5	Spo08328.1.CDS.5	CDS
Spo08328.1.CDS.4	Spo08328.1.CDS.4	CDS
Spo08328.1.CDS.3	Spo08328.1.CDS.3	CDS
Spo08328.1.CDS.2	Spo08328.1.CDS.2	CDS
Spo08328.1.CDS.1	Spo08328.1.CDS.1	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo08328.1.utr3p.1	Spo08328.1.utr3p.1	three_prime_UTR

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo08328.1.exon.9	Spo08328.1.exon.9	exon
Spo08328.1.exon.8	Spo08328.1.exon.8	exon
Spo08328.1.exon.7	Spo08328.1.exon.7	exon
Spo08328.1.exon.6	Spo08328.1.exon.6	exon
Spo08328.1.exon.5	Spo08328.1.exon.5	exon
Spo08328.1.exon.4	Spo08328.1.exon.4	exon
Spo08328.1.exon.3	Spo08328.1.exon.3	exon
Spo08328.1.exon.2	Spo08328.1.exon.2	exon
Spo08328.1.exon.1	Spo08328.1.exon.1	exon

Homology

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BLAST of Spo08328.1 vs. NCBI nr
Match: gi|902219780|gb|KNA18404.1| (hypothetical protein SOVF_070820 [Spinacia oleracea])

HSP 1 Score: 617.1 bits (1590), Expect = 1.600e-173
Identity = 298/298 (100.00%), Postives = 298/298 (100.00%), Query Frame = 1


		  
Query: 1   MGRVPIVNENYRRVIEAARRDLHRSLVQDNNNSAPILLRLSFHDAVDYDAATKRGGANGS 60
           MGRVPIVNENYRRVIEAARRDLHRSLVQDNNNSAPILLRLSFHDAVDYDAATKRGGANGS
Sbjct: 1   MGRVPIVNENYRRVIEAARRDLHRSLVQDNNNSAPILLRLSFHDAVDYDAATKRGGANGS 60

Query: 61  VRLAQELNRTPNKGIETAVRFCEPIKRRHPDITYADLYQLAGIVAVEVTGGPAIDFVPGR 120
           VRLAQELNRTPNKGIETAVRFCEPIKRRHPDITYADLYQLAGIVAVEVTGGPAIDFVPGR
Sbjct: 61  VRLAQELNRTPNKGIETAVRFCEPIKRRHPDITYADLYQLAGIVAVEVTGGPAIDFVPGR 120

Query: 121 ADADVADQDNIPNPRRGADHLRTVFYRMGLNDKDIVVLSGAHALGGAHKDRSGFDGDFTR 180
           ADADVADQDNIPNPRRGADHLRTVFYRMGLNDKDIVVLSGAHALGGAHKDRSGFDGDFTR
Sbjct: 121 ADADVADQDNIPNPRRGADHLRTVFYRMGLNDKDIVVLSGAHALGGAHKDRSGFDGDFTR 180

Query: 181 NPLTFDNSYFVELLRGDTPGLVKFPTDKALLTDPRFRPFVDLYARDQRAFFRDYAESHKK 240
           NPLTFDNSYFVELLRGDTPGLVKFPTDKALLTDPRFRPFVDLYARDQRAFFRDYAESHKK
Sbjct: 181 NPLTFDNSYFVELLRGDTPGLVKFPTDKALLTDPRFRPFVDLYARDQRAFFRDYAESHKK 240

Query: 241 MSLLGLNHPESNLYESNSCSDEVECGAQSYVVQNGGCPMQYGYARPYAVGNGCCPGGY 299
           MSLLGLNHPESNLYESNSCSDEVECGAQSYVVQNGGCPMQYGYARPYAVGNGCCPGGY
Sbjct: 241 MSLLGLNHPESNLYESNSCSDEVECGAQSYVVQNGGCPMQYGYARPYAVGNGCCPGGY 298

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BLAST of Spo08328.1 vs. NCBI nr
Match: gi|987701|dbj|BAA08535.1| (ascorbate peroxidase [Spinacia oleracea])

HSP 1 Score: 511.5 bits (1316), Expect = 9.700e-142
Identity = 253/260 (97.31%), Postives = 253/260 (97.31%), Query Frame = 1


		  
Query: 1   MGRVPIVNENYRRVIEAARRDLHRSLVQDNNNSAPILLRLSFHDAVDYDAATKRGGANGS 60
           MGRVPIVNENYRRVIEAARRDLHRSLVQDNNNSAPILLRLSFHDAVDYDAATKRGGANGS
Sbjct: 1   MGRVPIVNENYRRVIEAARRDLHRSLVQDNNNSAPILLRLSFHDAVDYDAATKRGGANGS 60

Query: 61  VRLAQELNRTPNKGIETAVRFCEPIKRRHPDITYADLYQLAGIVAVEVTGGPAIDFVPGR 120
           VRLAQELNRTPNKGIETAVRFCEPIKRRHPDITYADLYQLAGIVAVEVTGGPAID     
Sbjct: 61  VRLAQELNRTPNKGIETAVRFCEPIKRRHPDITYADLYQLAGIVAVEVTGGPAID----- 120

Query: 121 ADADVADQDNIPNPRRGADHLRTVFYRMGLNDKDIVVLSGAHALGGAHKDRSGFDGDFTR 180
             ADVADQDNIPNPRRGADHLRTVFYRMGLNDKDIVVLSGAHALGGAHKDRSGFDGDFTR
Sbjct: 121 --ADVADQDNIPNPRRGADHLRTVFYRMGLNDKDIVVLSGAHALGGAHKDRSGFDGDFTR 180

Query: 181 NPLTFDNSYFVELLRGDTPGLVKFPTDKALLTDPRFRPFVDLYARDQRAFFRDYAESHKK 240
           NPLTFDNSYFVELLRGDTPGLVKFPTDKALLTDPRFRPFVDLYARDQRAFFRDYAESHKK
Sbjct: 181 NPLTFDNSYFVELLRGDTPGLVKFPTDKALLTDPRFRPFVDLYARDQRAFFRDYAESHKK 240

Query: 241 MSLLGLNHPESNLYESNSCS 261
           MSLLGLNHPESNLYESNSCS
Sbjct: 241 MSLLGLNHPESNLYESNSCS 253

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BLAST of Spo08328.1 vs. NCBI nr
Match: gi|870858051|gb|KMT09577.1| (hypothetical protein BVRB_6g130330 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 483.4 bits (1243), Expect = 2.800e-133
Identity = 238/298 (79.87%), Postives = 258/298 (86.58%), Query Frame = 1


		  
Query: 1   MGRVPIVNENYRRVIEAARRDLHRSLVQDNNNSAPILLRLSFHDAVDYDAATKRGGANGS 60
           MGRVP+VNENY RVIEAARRDL RS++Q+N N+APILLRLSFHDA +YDA  KRGGANGS
Sbjct: 1   MGRVPVVNENYCRVIEAARRDL-RSVIQNNKNAAPILLRLSFHDAGNYDARAKRGGANGS 60

Query: 61  VRLAQELNRTPNKGIETAVRFCEPIKRRHPDITYADLYQLAGIVAVEVTGGPAIDFVPGR 120
           VR  QELNR  NKG+E A+R CEP+KRRHPDITYADLYQLAGIVAVEVTGGPAI+FVPGR
Sbjct: 61  VRFTQELNRPVNKGMENAIRLCEPVKRRHPDITYADLYQLAGIVAVEVTGGPAIEFVPGR 120

Query: 121 ADADVADQDNIPNPRRGADHLRTVFYRMGLNDKDIVVLSGAHALGGAHKDRSGFDGDFTR 180
           AD D  D D IPNPR G DHLRT FYRMGLNDKDIVVLSGAHALGGAHKDRSGFDG+FT+
Sbjct: 121 ADIDSPDADGIPNPRGGVDHLRTTFYRMGLNDKDIVVLSGAHALGGAHKDRSGFDGNFTQ 180

Query: 181 NPLTFDNSYFVELLRGDTPGLVKFPTDKALLTDPRFRPFVDLYARDQRAFFRDYAESHKK 240
           NPL FDNSYFVELLRGDTPGLVK PTDKALL D  FR FV+LYARDQRAFFRDYAESHKK
Sbjct: 181 NPLKFDNSYFVELLRGDTPGLVKLPTDKALLNDSVFRRFVELYARDQRAFFRDYAESHKK 240

Query: 241 MSLLGLNHPESNLYESNSCSDEVECGAQSYVVQNGGCPMQYGYARPYAVGNGCCPGGY 299
           +S LG NH ES+LYE +SC + V C AQSY V++G CP Q+GYARPYAVGNG CP  Y
Sbjct: 241 LSELGFNHQESDLYEDDSCDESVGC-AQSYAVEDGCCPAQFGYARPYAVGNG-CPANY 295

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BLAST of Spo08328.1 vs. NCBI nr
Match: gi|870858054|gb|KMT09580.1| (hypothetical protein BVRB_6g130360 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 373.6 bits (958), Expect = 3.200e-100
Identity = 180/224 (80.36%), Postives = 194/224 (86.61%), Query Frame = 1


		  
Query: 75  IETAVRFCEPIKRRHPDITYADLYQLAGIVAVEVTGGPAIDFVPGRADADVADQDNIPNP 134
           +E A+R CEP+KRRHPDITYADLYQLAGIVAVEVTGGPAI+FVPGRAD D  D D IPNP
Sbjct: 1   MENAIRLCEPVKRRHPDITYADLYQLAGIVAVEVTGGPAIEFVPGRADIDSPDADGIPNP 60

Query: 135 RRGADHLRTVFYRMGLNDKDIVVLSGAHALGGAHKDRSGFDGDFTRNPLTFDNSYFVELL 194
           R G DHLRT FYRMGLNDKDIVVLSGAHALGGAHKDRSGFDG+FT+NPL FDNSYFVELL
Sbjct: 61  RGGVDHLRTTFYRMGLNDKDIVVLSGAHALGGAHKDRSGFDGNFTQNPLKFDNSYFVELL 120

Query: 195 RGDTPGLVKFPTDKALLTDPRFRPFVDLYARDQRAFFRDYAESHKKMSLLGLNHPESNLY 254
           RGDTPGLVK PTDKALL D  FR FV+LYARDQRAFFRDYAESHKK+S LG NH ES+LY
Sbjct: 121 RGDTPGLVKLPTDKALLNDSVFRRFVELYARDQRAFFRDYAESHKKLSELGFNHQESDLY 180

Query: 255 ESNSCSDEVECGAQSYVVQNGGCPMQYGYARPYAVGNGCCPGGY 299
           E +SC + V C AQSY V++G CP Q+GYARPYAVGNG CP  Y
Sbjct: 181 EDDSCDESVGC-AQSYAVEDGCCPAQFGYARPYAVGNG-CPANY 222

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BLAST of Spo08328.1 vs. NCBI nr
Match: gi|3377755|gb|AAC28103.1| (ascorbate peroxidase [Mesembryanthemum crystallinum])

HSP 1 Score: 341.3 bits (874), Expect = 1.700e-90
Identity = 162/241 (67.22%), Postives = 198/241 (82.16%), Query Frame = 1


		  
Query: 5   PIVNENYRRVIEAARRDLHRSLVQDNNNSAPILLRLSFHDAVDYDAATKRGGANGSVRLA 64
           P+V++ Y + +E ARRDL  S++Q   N+AP+LLRL+FHDA +Y+     GG NGSVRL 
Sbjct: 5   PVVDQRYLKDLEGARRDL-ASIIQ-RKNAAPVLLRLAFHDAANYNVTNNTGGVNGSVRLR 64

Query: 65  QELNRTPNKGIETAVRFCEPIKRRHPDITYADLYQLAGIVAVEVTGGPAIDFVPGRADAD 124
           QEL++ PNKGIE  V+FCE +K++HP +TYAD+ QLAG++AVE++GGP IDFVPGR D +
Sbjct: 65  QELSQPPNKGIEDGVKFCEEVKKKHPRVTYADIIQLAGVLAVELSGGPCIDFVPGRMDTN 124

Query: 125 VADQDNIPNPRRGADHLRTVFYRMGLNDKDIVVLSGAHALGGAHKDRSGFDGDFTRNPLT 184
           VAD+ NIPNPR GADHLR  FY+MGL+DKDIVVLSGAH LG A K+ SGF+G FTRN L 
Sbjct: 125 VADKLNIPNPRGGADHLRRTFYQMGLSDKDIVVLSGAHTLGRARKENSGFNGPFTRNTLK 184

Query: 185 FDNSYFVELLRGDTPGLVKFPTDKALLTDPRFRPFVDLYARDQRAFFRDYAESHKKMSLL 244
           FDNSYFVEL+RG+TPGLVKFPTDKAL+ DP FRP V+LYAR + AFFRDYAESHKK+S L
Sbjct: 185 FDNSYFVELMRGETPGLVKFPTDKALVQDPVFRPLVELYARHEGAFFRDYAESHKKLSEL 243

Query: 245 G 246
           G
Sbjct: 245 G 243

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BLAST of Spo08328.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RHH7_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_070820 PE=3 SV=1)

HSP 1 Score: 617.1 bits (1590), Expect = 1.100e-173
Identity = 298/298 (100.00%), Postives = 298/298 (100.00%), Query Frame = 1


		  
Query: 1   MGRVPIVNENYRRVIEAARRDLHRSLVQDNNNSAPILLRLSFHDAVDYDAATKRGGANGS 60
           MGRVPIVNENYRRVIEAARRDLHRSLVQDNNNSAPILLRLSFHDAVDYDAATKRGGANGS
Sbjct: 1   MGRVPIVNENYRRVIEAARRDLHRSLVQDNNNSAPILLRLSFHDAVDYDAATKRGGANGS 60

Query: 61  VRLAQELNRTPNKGIETAVRFCEPIKRRHPDITYADLYQLAGIVAVEVTGGPAIDFVPGR 120
           VRLAQELNRTPNKGIETAVRFCEPIKRRHPDITYADLYQLAGIVAVEVTGGPAIDFVPGR
Sbjct: 61  VRLAQELNRTPNKGIETAVRFCEPIKRRHPDITYADLYQLAGIVAVEVTGGPAIDFVPGR 120

Query: 121 ADADVADQDNIPNPRRGADHLRTVFYRMGLNDKDIVVLSGAHALGGAHKDRSGFDGDFTR 180
           ADADVADQDNIPNPRRGADHLRTVFYRMGLNDKDIVVLSGAHALGGAHKDRSGFDGDFTR
Sbjct: 121 ADADVADQDNIPNPRRGADHLRTVFYRMGLNDKDIVVLSGAHALGGAHKDRSGFDGDFTR 180

Query: 181 NPLTFDNSYFVELLRGDTPGLVKFPTDKALLTDPRFRPFVDLYARDQRAFFRDYAESHKK 240
           NPLTFDNSYFVELLRGDTPGLVKFPTDKALLTDPRFRPFVDLYARDQRAFFRDYAESHKK
Sbjct: 181 NPLTFDNSYFVELLRGDTPGLVKFPTDKALLTDPRFRPFVDLYARDQRAFFRDYAESHKK 240

Query: 241 MSLLGLNHPESNLYESNSCSDEVECGAQSYVVQNGGCPMQYGYARPYAVGNGCCPGGY 299
           MSLLGLNHPESNLYESNSCSDEVECGAQSYVVQNGGCPMQYGYARPYAVGNGCCPGGY
Sbjct: 241 MSLLGLNHPESNLYESNSCSDEVECGAQSYVVQNGGCPMQYGYARPYAVGNGCCPGGY 298

[Close]

BLAST of Spo08328.1 vs. UniProtKB/TrEMBL
Match: Q41371_SPIOL (Ascorbate peroxidase OS=Spinacia oleracea PE=2 SV=1)

HSP 1 Score: 511.5 bits (1316), Expect = 6.800e-142
Identity = 253/260 (97.31%), Postives = 253/260 (97.31%), Query Frame = 1


		  
Query: 1   MGRVPIVNENYRRVIEAARRDLHRSLVQDNNNSAPILLRLSFHDAVDYDAATKRGGANGS 60
           MGRVPIVNENYRRVIEAARRDLHRSLVQDNNNSAPILLRLSFHDAVDYDAATKRGGANGS
Sbjct: 1   MGRVPIVNENYRRVIEAARRDLHRSLVQDNNNSAPILLRLSFHDAVDYDAATKRGGANGS 60

Query: 61  VRLAQELNRTPNKGIETAVRFCEPIKRRHPDITYADLYQLAGIVAVEVTGGPAIDFVPGR 120
           VRLAQELNRTPNKGIETAVRFCEPIKRRHPDITYADLYQLAGIVAVEVTGGPAID     
Sbjct: 61  VRLAQELNRTPNKGIETAVRFCEPIKRRHPDITYADLYQLAGIVAVEVTGGPAID----- 120

Query: 121 ADADVADQDNIPNPRRGADHLRTVFYRMGLNDKDIVVLSGAHALGGAHKDRSGFDGDFTR 180
             ADVADQDNIPNPRRGADHLRTVFYRMGLNDKDIVVLSGAHALGGAHKDRSGFDGDFTR
Sbjct: 121 --ADVADQDNIPNPRRGADHLRTVFYRMGLNDKDIVVLSGAHALGGAHKDRSGFDGDFTR 180

Query: 181 NPLTFDNSYFVELLRGDTPGLVKFPTDKALLTDPRFRPFVDLYARDQRAFFRDYAESHKK 240
           NPLTFDNSYFVELLRGDTPGLVKFPTDKALLTDPRFRPFVDLYARDQRAFFRDYAESHKK
Sbjct: 181 NPLTFDNSYFVELLRGDTPGLVKFPTDKALLTDPRFRPFVDLYARDQRAFFRDYAESHKK 240

Query: 241 MSLLGLNHPESNLYESNSCS 261
           MSLLGLNHPESNLYESNSCS
Sbjct: 241 MSLLGLNHPESNLYESNSCS 253

[Close]

BLAST of Spo08328.1 vs. UniProtKB/TrEMBL
Match: A0A0J8C7N2_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_6g130330 PE=3 SV=1)

HSP 1 Score: 483.4 bits (1243), Expect = 2.000e-133
Identity = 238/298 (79.87%), Postives = 258/298 (86.58%), Query Frame = 1


		  
Query: 1   MGRVPIVNENYRRVIEAARRDLHRSLVQDNNNSAPILLRLSFHDAVDYDAATKRGGANGS 60
           MGRVP+VNENY RVIEAARRDL RS++Q+N N+APILLRLSFHDA +YDA  KRGGANGS
Sbjct: 1   MGRVPVVNENYCRVIEAARRDL-RSVIQNNKNAAPILLRLSFHDAGNYDARAKRGGANGS 60

Query: 61  VRLAQELNRTPNKGIETAVRFCEPIKRRHPDITYADLYQLAGIVAVEVTGGPAIDFVPGR 120
           VR  QELNR  NKG+E A+R CEP+KRRHPDITYADLYQLAGIVAVEVTGGPAI+FVPGR
Sbjct: 61  VRFTQELNRPVNKGMENAIRLCEPVKRRHPDITYADLYQLAGIVAVEVTGGPAIEFVPGR 120

Query: 121 ADADVADQDNIPNPRRGADHLRTVFYRMGLNDKDIVVLSGAHALGGAHKDRSGFDGDFTR 180
           AD D  D D IPNPR G DHLRT FYRMGLNDKDIVVLSGAHALGGAHKDRSGFDG+FT+
Sbjct: 121 ADIDSPDADGIPNPRGGVDHLRTTFYRMGLNDKDIVVLSGAHALGGAHKDRSGFDGNFTQ 180

Query: 181 NPLTFDNSYFVELLRGDTPGLVKFPTDKALLTDPRFRPFVDLYARDQRAFFRDYAESHKK 240
           NPL FDNSYFVELLRGDTPGLVK PTDKALL D  FR FV+LYARDQRAFFRDYAESHKK
Sbjct: 181 NPLKFDNSYFVELLRGDTPGLVKLPTDKALLNDSVFRRFVELYARDQRAFFRDYAESHKK 240

Query: 241 MSLLGLNHPESNLYESNSCSDEVECGAQSYVVQNGGCPMQYGYARPYAVGNGCCPGGY 299
           +S LG NH ES+LYE +SC + V C AQSY V++G CP Q+GYARPYAVGNG CP  Y
Sbjct: 241 LSELGFNHQESDLYEDDSCDESVGC-AQSYAVEDGCCPAQFGYARPYAVGNG-CPANY 295

[Close]

BLAST of Spo08328.1 vs. UniProtKB/TrEMBL
Match: A0A0J8C797_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_6g130360 PE=3 SV=1)

HSP 1 Score: 373.6 bits (958), Expect = 2.200e-100
Identity = 180/224 (80.36%), Postives = 194/224 (86.61%), Query Frame = 1


		  
Query: 75  IETAVRFCEPIKRRHPDITYADLYQLAGIVAVEVTGGPAIDFVPGRADADVADQDNIPNP 134
           +E A+R CEP+KRRHPDITYADLYQLAGIVAVEVTGGPAI+FVPGRAD D  D D IPNP
Sbjct: 1   MENAIRLCEPVKRRHPDITYADLYQLAGIVAVEVTGGPAIEFVPGRADIDSPDADGIPNP 60

Query: 135 RRGADHLRTVFYRMGLNDKDIVVLSGAHALGGAHKDRSGFDGDFTRNPLTFDNSYFVELL 194
           R G DHLRT FYRMGLNDKDIVVLSGAHALGGAHKDRSGFDG+FT+NPL FDNSYFVELL
Sbjct: 61  RGGVDHLRTTFYRMGLNDKDIVVLSGAHALGGAHKDRSGFDGNFTQNPLKFDNSYFVELL 120

Query: 195 RGDTPGLVKFPTDKALLTDPRFRPFVDLYARDQRAFFRDYAESHKKMSLLGLNHPESNLY 254
           RGDTPGLVK PTDKALL D  FR FV+LYARDQRAFFRDYAESHKK+S LG NH ES+LY
Sbjct: 121 RGDTPGLVKLPTDKALLNDSVFRRFVELYARDQRAFFRDYAESHKKLSELGFNHQESDLY 180

Query: 255 ESNSCSDEVECGAQSYVVQNGGCPMQYGYARPYAVGNGCCPGGY 299
           E +SC + V C AQSY V++G CP Q+GYARPYAVGNG CP  Y
Sbjct: 181 EDDSCDESVGC-AQSYAVEDGCCPAQFGYARPYAVGNG-CPANY 222

[Close]

BLAST of Spo08328.1 vs. UniProtKB/TrEMBL
Match: O81604_MESCR (Ascorbate peroxidase OS=Mesembryanthemum crystallinum PE=2 SV=1)

HSP 1 Score: 341.3 bits (874), Expect = 1.200e-90
Identity = 162/241 (67.22%), Postives = 198/241 (82.16%), Query Frame = 1


		  
Query: 5   PIVNENYRRVIEAARRDLHRSLVQDNNNSAPILLRLSFHDAVDYDAATKRGGANGSVRLA 64
           P+V++ Y + +E ARRDL  S++Q   N+AP+LLRL+FHDA +Y+     GG NGSVRL 
Sbjct: 5   PVVDQRYLKDLEGARRDL-ASIIQ-RKNAAPVLLRLAFHDAANYNVTNNTGGVNGSVRLR 64

Query: 65  QELNRTPNKGIETAVRFCEPIKRRHPDITYADLYQLAGIVAVEVTGGPAIDFVPGRADAD 124
           QEL++ PNKGIE  V+FCE +K++HP +TYAD+ QLAG++AVE++GGP IDFVPGR D +
Sbjct: 65  QELSQPPNKGIEDGVKFCEEVKKKHPRVTYADIIQLAGVLAVELSGGPCIDFVPGRMDTN 124

Query: 125 VADQDNIPNPRRGADHLRTVFYRMGLNDKDIVVLSGAHALGGAHKDRSGFDGDFTRNPLT 184
           VAD+ NIPNPR GADHLR  FY+MGL+DKDIVVLSGAH LG A K+ SGF+G FTRN L 
Sbjct: 125 VADKLNIPNPRGGADHLRRTFYQMGLSDKDIVVLSGAHTLGRARKENSGFNGPFTRNTLK 184

Query: 185 FDNSYFVELLRGDTPGLVKFPTDKALLTDPRFRPFVDLYARDQRAFFRDYAESHKKMSLL 244
           FDNSYFVEL+RG+TPGLVKFPTDKAL+ DP FRP V+LYAR + AFFRDYAESHKK+S L
Sbjct: 185 FDNSYFVELMRGETPGLVKFPTDKALVQDPVFRPLVELYARHEGAFFRDYAESHKKLSEL 243

Query: 245 G 246
           G
Sbjct: 245 G 243

[Close]

BLAST of Spo08328.1 vs. ExPASy Swiss-Prot
Match: APX5_ARATH (L-ascorbate peroxidase 5, peroxisomal OS=Arabidopsis thaliana GN=APX5 PE=1 SV=2)

HSP 1 Score: 316.6 bits (810), Expect = 2.900e-85
Identity = 154/243 (63.37%), Postives = 187/243 (76.95%), Query Frame = 1


		  
Query: 7   VNENYRRVIEAARRDLHRSLVQDNNNSAPILLRLSFHDAVDYDAATKRGGANGSVRLAQE 66
           V+  Y + IE  RRDL R+L+  + N API+LRL++HDA  YDA  K GGANGS+R  +E
Sbjct: 5   VDAEYLKEIEKTRRDL-RALIS-SRNCAPIMLRLAWHDAGTYDAKKKTGGANGSIRFKEE 64

Query: 67  LNRTPNKGIETAVRFCEPIKRRHPDITYADLYQLAGIVAVEVTGGPAIDFVPGRADADVA 126
           LNR  NKG+E AV FCE +K +HP ++YADLYQLAG+VAVEVTGGPAI F PGR DAD A
Sbjct: 65  LNRPHNKGLEKAVAFCEEVKAKHPRVSYADLYQLAGVVAVEVTGGPAIPFTPGRKDADSA 124

Query: 127 DQDNIPNPRRGADHLRTVFYRMGLNDKDIVVLSGAHALGGAHKDRSGFDGDFTRNPLTFD 186
           D   +PNP  GA HLRT+F RMGL D+DIV LSG H LG AHK+RS F+G +T++PL FD
Sbjct: 125 DDGELPNPNEGASHLRTLFSRMGLLDRDIVALSGGHTLGRAHKERSDFEGPWTQDPLKFD 184

Query: 187 NSYFVELLRGDTPGLVKFPTDKALLTDPRFRPFVDLYARDQRAFFRDYAESHKKMSLLGL 246
           NSYFVELL+G+TPGL++  TDKALL DP+F PFV LYA+D+  FF+ YA SHKK+S LG 
Sbjct: 185 NSYFVELLKGETPGLLQLKTDKALLDDPKFHPFVKLYAKDEDMFFKAYAISHKKLSELGF 244

Query: 247 NHP 250
           N P
Sbjct: 245 NPP 245

[Close]

BLAST of Spo08328.1 vs. ExPASy Swiss-Prot
Match: APX3_ORYSI (Probable L-ascorbate peroxidase 3 OS=Oryza sativa subsp. indica GN=APX3 PE=2 SV=1)

HSP 1 Score: 307.0 bits (785), Expect = 2.300e-82
Identity = 150/252 (59.52%), Postives = 191/252 (75.79%), Query Frame = 1


		  
Query: 1   MGRVPIVNENYRRVIEAARRDLHRSLVQDNNNSAPILLRLSFHDAVDYDAATKRGGANGS 60
           M   P+V+  Y   +E ARRDL R+L+  + + API+LRL++HDA  YD ATK GG NGS
Sbjct: 1   MSAAPVVDAEYMAEVERARRDL-RALIA-SKSCAPIMLRLAWHDAGTYDKATKTGGPNGS 60

Query: 61  VRLAQELNRTPNKGIETAVRFCEPIKRRHPDITYADLYQLAGIVAVEVTGGPAIDFVPGR 120
           +R  QE +   N GI+ A+   EP+K+RHP ITYADLYQLAG+VAVEVTGGP ID+VPGR
Sbjct: 61  IRFPQEYSHAANAGIKIAIDLLEPMKQRHPKITYADLYQLAGVVAVEVTGGPTIDYVPGR 120

Query: 121 ADA-DVADQDNIPNPRRGADHLRTVFYRMGLNDKDIVVLSGAHALGGAHKDRSGFDGDFT 180
            D+ D  ++  +P+ ++GA HLR VFYRMGL+DKDIV LSG H LG A  +RSGFDG +T
Sbjct: 121 RDSSDSPEEGRLPDAKKGAAHLREVFYRMGLSDKDIVALSGGHTLGKARPERSGFDGAWT 180

Query: 181 RNPLTFDNSYFVELLRGDTPGLVKFPTDKALLTDPRFRPFVDLYARDQRAFFRDYAESHK 240
           ++PL FDNSYF+ELL+ ++ GL+K PTDKAL+ DP FR +V+LYA+D+ AFFRDYAESHK
Sbjct: 181 KDPLKFDNSYFIELLKENSEGLLKLPTDKALVEDPTFRRYVELYAKDEDAFFRDYAESHK 240

Query: 241 KMSLLGLNHPES 252
           K+S LG   P S
Sbjct: 241 KLSELGFTPPRS 250

[Close]

BLAST of Spo08328.1 vs. ExPASy Swiss-Prot
Match: APX3_ORYSJ (Probable L-ascorbate peroxidase 3 OS=Oryza sativa subsp. japonica GN=APX3 PE=3 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 5.100e-82
Identity = 149/252 (59.13%), Postives = 191/252 (75.79%), Query Frame = 1


		  
Query: 1   MGRVPIVNENYRRVIEAARRDLHRSLVQDNNNSAPILLRLSFHDAVDYDAATKRGGANGS 60
           M   P+V+  Y   +E ARRDL R+L+  + + API+LRL++HDA  YD ATK GG NGS
Sbjct: 1   MSAAPVVDAEYMAEVERARRDL-RALIA-SKSCAPIMLRLAWHDAGTYDKATKTGGPNGS 60

Query: 61  VRLAQELNRTPNKGIETAVRFCEPIKRRHPDITYADLYQLAGIVAVEVTGGPAIDFVPGR 120
           +R  QE +   N GI+ A+   EP+K++HP ITYADLYQLAG+VAVEVTGGP ID+VPGR
Sbjct: 61  IRFPQEYSHAANAGIKIAIDLLEPMKQKHPKITYADLYQLAGVVAVEVTGGPTIDYVPGR 120

Query: 121 ADA-DVADQDNIPNPRRGADHLRTVFYRMGLNDKDIVVLSGAHALGGAHKDRSGFDGDFT 180
            D+ D  ++  +P+ ++GA HLR VFYRMGL+DKDIV LSG H LG A  +RSGFDG +T
Sbjct: 121 RDSSDSPEEGRLPDAKKGAAHLREVFYRMGLSDKDIVALSGGHTLGKARPERSGFDGAWT 180

Query: 181 RNPLTFDNSYFVELLRGDTPGLVKFPTDKALLTDPRFRPFVDLYARDQRAFFRDYAESHK 240
           ++PL FDNSYF+ELL+ ++ GL+K PTDKAL+ DP FR +V+LYA+D+ AFFRDYAESHK
Sbjct: 181 KDPLKFDNSYFIELLKENSEGLLKLPTDKALVEDPTFRRYVELYAKDEDAFFRDYAESHK 240

Query: 241 KMSLLGLNHPES 252
           K+S LG   P S
Sbjct: 241 KLSELGFTPPRS 250

[Close]

BLAST of Spo08328.1 vs. ExPASy Swiss-Prot
Match: APX3_ARATH (L-ascorbate peroxidase 3, peroxisomal OS=Arabidopsis thaliana GN=APX3 PE=1 SV=1)

HSP 1 Score: 299.7 bits (766), Expect = 3.600e-80
Identity = 154/280 (55.00%), Postives = 196/280 (70.00%), Query Frame = 1


		  
Query: 5   PIVNENYRRVIEAARRDLHRSLVQDNNNSAPILLRLSFHDAVDYDAATKRGGANGSVRLA 64
           PIV+  Y + I  ARR+L RSL+  N N API+LRL++HDA  YDA +K GG NGS+R  
Sbjct: 4   PIVDAEYLKEITKARREL-RSLIA-NKNCAPIMLRLAWHDAGTYDAQSKTGGPNGSIRNE 63

Query: 65  QELNRTPNKGIETAVRFCEPIKRRHPDITYADLYQLAGIVAVEVTGGPAIDFVPGRADAD 124
           +E     N G++ A+  CE +K +HP ITYADLYQLAG+VAVEVTGGP I FVPGR D++
Sbjct: 64  EEHTHGANSGLKIALDLCEGVKAKHPKITYADLYQLAGVVAVEVTGGPDIVFVPGRKDSN 123

Query: 125 VADQDN-IPNPRRGADHLRTVFYRMGLNDKDIVVLSGAHALGGAHKDRSGFDGDFTRNPL 184
           V  ++  +P+ ++G  HLR VFYRMGL+DKDIV LSG H LG AH +RSGFDG +T+ PL
Sbjct: 124 VCPKEGRLPDAKQGFQHLRDVFYRMGLSDKDIVALSGGHTLGRAHPERSGFDGPWTQEPL 183

Query: 185 TFDNSYFVELLRGDTPGLVKFPTDKALLTDPRFRPFVDLYARDQRAFFRDYAESHKKMSL 244
            FDNSYFVELL+G++ GL+K PTDK LL DP FR  V+LYA+D+ AFFRDYAESHKK+S 
Sbjct: 184 KFDNSYFVELLKGESEGLLKLPTDKTLLEDPEFRRLVELYAKDEDAFFRDYAESHKKLSE 243

Query: 245 LGLNHPESNLYESNSCSDEVECGAQSYVVQNGGCPMQYGY 284
           LG N    N     + +D       ++ V      + +GY
Sbjct: 244 LGFN---PNSSAGKAVADSTILAQSAFGVAVAAAVVAFGY 278

[Close]

BLAST of Spo08328.1 vs. ExPASy Swiss-Prot
Match: APX4_ORYSJ (Probable L-ascorbate peroxidase 4 OS=Oryza sativa subsp. japonica GN=APX4 PE=2 SV=1)

HSP 1 Score: 294.7 bits (753), Expect = 1.200e-78
Identity = 142/242 (58.68%), Postives = 184/242 (76.03%), Query Frame = 1


		  
Query: 5   PIVNENYRRVIEAARRDLHRSLVQDNNNSAPILLRLSFHDAVDYDAATKRGGANGSVRLA 64
           P+V+  Y R ++ ARR L R+L+  +   API+LRL++HDA  YD  TK GGANGS+R  
Sbjct: 4   PVVDAEYLRQVDRARRHL-RALIS-SKGCAPIMLRLAWHDAGTYDVNTKTGGANGSIRYE 63

Query: 65  QELNRTPNKGIETAVRFCEPIKRRHPDITYADLYQLAGIVAVEVTGGPAIDFVPGRADAD 124
           +E     N G++ A+   EPIK + P ITYADLYQLAG+VAVEVTGGP ++F+PGR D+ 
Sbjct: 64  EEYTHGSNAGLKIAIDLLEPIKAKSPKITYADLYQLAGVVAVEVTGGPTVEFIPGRRDSS 123

Query: 125 VADQDN-IPNPRRGADHLRTVFYRMGLNDKDIVVLSGAHALGGAHKDRSGFDGDFTRNPL 184
           V  ++  +P+ ++GA HLR +FYRMGL+DKDIV LSG H LG AH +RSGF+G +T+ PL
Sbjct: 124 VCPREGRLPDAKKGALHLRDIFYRMGLSDKDIVALSGGHTLGRAHPERSGFEGAWTQEPL 183

Query: 185 TFDNSYFVELLRGDTPGLVKFPTDKALLTDPRFRPFVDLYARDQRAFFRDYAESHKKMSL 244
            FDNSYF+ELL+G++ GL+K PTDKALL DP FR +VDLYARD+  FF+DYAESHKK+S 
Sbjct: 184 KFDNSYFLELLKGESEGLLKLPTDKALLEDPSFRRYVDLYARDEDTFFKDYAESHKKLSE 243

Query: 245 LG 246
           LG
Sbjct: 244 LG 243

[Close]

BLAST of Spo08328.1 vs. TAIR (Arabidopsis)
Match: AT4G35970.1 (ascorbate peroxidase 5)

HSP 1 Score: 316.6 bits (810), Expect = 1.600e-86
Identity = 154/243 (63.37%), Postives = 187/243 (76.95%), Query Frame = 1


		  
Query: 7   VNENYRRVIEAARRDLHRSLVQDNNNSAPILLRLSFHDAVDYDAATKRGGANGSVRLAQE 66
           V+  Y + IE  RRDL R+L+  + N API+LRL++HDA  YDA  K GGANGS+R  +E
Sbjct: 5   VDAEYLKEIEKTRRDL-RALIS-SRNCAPIMLRLAWHDAGTYDAKKKTGGANGSIRFKEE 64

Query: 67  LNRTPNKGIETAVRFCEPIKRRHPDITYADLYQLAGIVAVEVTGGPAIDFVPGRADADVA 126
           LNR  NKG+E AV FCE +K +HP ++YADLYQLAG+VAVEVTGGPAI F PGR DAD A
Sbjct: 65  LNRPHNKGLEKAVAFCEEVKAKHPRVSYADLYQLAGVVAVEVTGGPAIPFTPGRKDADSA 124

Query: 127 DQDNIPNPRRGADHLRTVFYRMGLNDKDIVVLSGAHALGGAHKDRSGFDGDFTRNPLTFD 186
           D   +PNP  GA HLRT+F RMGL D+DIV LSG H LG AHK+RS F+G +T++PL FD
Sbjct: 125 DDGELPNPNEGASHLRTLFSRMGLLDRDIVALSGGHTLGRAHKERSDFEGPWTQDPLKFD 184

Query: 187 NSYFVELLRGDTPGLVKFPTDKALLTDPRFRPFVDLYARDQRAFFRDYAESHKKMSLLGL 246
           NSYFVELL+G+TPGL++  TDKALL DP+F PFV LYA+D+  FF+ YA SHKK+S LG 
Sbjct: 185 NSYFVELLKGETPGLLQLKTDKALLDDPKFHPFVKLYAKDEDMFFKAYAISHKKLSELGF 244

Query: 247 NHP 250
           N P
Sbjct: 245 NPP 245

[Close]

BLAST of Spo08328.1 vs. TAIR (Arabidopsis)
Match: AT4G35000.1 (ascorbate peroxidase 3)

HSP 1 Score: 299.7 bits (766), Expect = 2.000e-81
Identity = 154/280 (55.00%), Postives = 196/280 (70.00%), Query Frame = 1


		  
Query: 5   PIVNENYRRVIEAARRDLHRSLVQDNNNSAPILLRLSFHDAVDYDAATKRGGANGSVRLA 64
           PIV+  Y + I  ARR+L RSL+  N N API+LRL++HDA  YDA +K GG NGS+R  
Sbjct: 4   PIVDAEYLKEITKARREL-RSLIA-NKNCAPIMLRLAWHDAGTYDAQSKTGGPNGSIRNE 63

Query: 65  QELNRTPNKGIETAVRFCEPIKRRHPDITYADLYQLAGIVAVEVTGGPAIDFVPGRADAD 124
           +E     N G++ A+  CE +K +HP ITYADLYQLAG+VAVEVTGGP I FVPGR D++
Sbjct: 64  EEHTHGANSGLKIALDLCEGVKAKHPKITYADLYQLAGVVAVEVTGGPDIVFVPGRKDSN 123

Query: 125 VADQDN-IPNPRRGADHLRTVFYRMGLNDKDIVVLSGAHALGGAHKDRSGFDGDFTRNPL 184
           V  ++  +P+ ++G  HLR VFYRMGL+DKDIV LSG H LG AH +RSGFDG +T+ PL
Sbjct: 124 VCPKEGRLPDAKQGFQHLRDVFYRMGLSDKDIVALSGGHTLGRAHPERSGFDGPWTQEPL 183

Query: 185 TFDNSYFVELLRGDTPGLVKFPTDKALLTDPRFRPFVDLYARDQRAFFRDYAESHKKMSL 244
            FDNSYFVELL+G++ GL+K PTDK LL DP FR  V+LYA+D+ AFFRDYAESHKK+S 
Sbjct: 184 KFDNSYFVELLKGESEGLLKLPTDKTLLEDPEFRRLVELYAKDEDAFFRDYAESHKKLSE 243

Query: 245 LGLNHPESNLYESNSCSDEVECGAQSYVVQNGGCPMQYGY 284
           LG N    N     + +D       ++ V      + +GY
Sbjct: 244 LGFN---PNSSAGKAVADSTILAQSAFGVAVAAAVVAFGY 278

[Close]

BLAST of Spo08328.1 vs. TAIR (Arabidopsis)
Match: AT3G09640.1 (ascorbate peroxidase 2)

HSP 1 Score: 260.8 bits (665), Expect = 1.100e-69
Identity = 131/247 (53.04%), Postives = 167/247 (67.61%), Query Frame = 1


		  
Query: 5   PIVNENYRRVIEAARRDLHRSLVQDNNNSAPILLRLSFHDAVDYDAATKRGGANGSVRLA 64
           P V E Y++ ++  +R L R L+ + +  API+LRL++H A  +D  TK GG  G++R  
Sbjct: 7   PEVKEEYKKAVQRCKRKL-RGLIAEKH-CAPIVLRLAWHSAGTFDVKTKTGGPFGTIRHP 66

Query: 65  QELNRTPNKGIETAVRFCEPIKRRHPDITYADLYQLAGIVAVEVTGGPAIDFVPGRAD-A 124
           QEL    N G++ AVR  +PIK   P ++YAD YQLAG+VAVE+TGGP I F PGR D  
Sbjct: 67  QELAHDANNGLDIAVRLLDPIKELFPILSYADFYQLAGVVAVEITGGPEIPFHPGRLDKV 126

Query: 125 DVADQDNIPNPRRGADHLRTVFYRMGLNDKDIVVLSGAHALGGAHKDRSGFDGDFTRNPL 184
           +   +  +P   +G DHLR VF RMGLNDKDIV LSG H LG  HK+RSGF+G +T NPL
Sbjct: 127 EPPPEGRLPQATKGVDHLRDVFGRMGLNDKDIVALSGGHTLGRCHKERSGFEGAWTPNPL 186

Query: 185 TFDNSYFVELLRGDTPGLVKFPTDKALLTDPRFRPFVDLYARDQRAFFRDYAESHKKMSL 244
            FDNSYF E+L G+  GL++ PTDKALL DP F PFV+ YA D+ AFF DY E+H K+S 
Sbjct: 187 IFDNSYFKEILSGEKEGLLQLPTDKALLDDPLFLPFVEKYAADEDAFFEDYTEAHLKLSE 246

Query: 245 LGLNHPE 251
           LG    E
Sbjct: 247 LGFADKE 251

[Close]

BLAST of Spo08328.1 vs. TAIR (Arabidopsis)
Match: AT1G07890.3 (ascorbate peroxidase 1)

HSP 1 Score: 247.7 bits (631), Expect = 9.200e-66
Identity = 127/243 (52.26%), Postives = 167/243 (68.72%), Query Frame = 1


		  
Query: 5   PIVNENYRRVIEAARRDLHRSLVQDNNNSAPILLRLSFHDAVDYDAATKRGGANGSVRLA 64
           P V+E+Y++ +E  RR L R L+ + N  API++RL++H A  +D  ++ GG  G++R  
Sbjct: 6   PTVSEDYKKAVEKCRRKL-RGLIAEKN-CAPIMVRLAWHSAGTFDCQSRTGGPFGTMRFD 65

Query: 65  QELNRTPNKGIETAVRFCEPIKRRHPDITYADLYQLAGIVAVEVTGGPAIDFVPGRADA- 124
            E     N GI  A+R  +PI+ + P I++AD +QLAG+VAVEVTGGP I F PGR D  
Sbjct: 66  AEQAHGANSGIHIALRLLDPIREQFPTISFADFHQLAGVVAVEVTGGPDIPFHPGREDKP 125

Query: 125 DVADQDNIPNPRRGADHLRTVFYR-MGLNDKDIVVLSGAHALGGAHKDRSGFDGDFTRNP 184
               +  +P+  +G DHLR VF + MGL+DKDIV LSGAH LG  HKDRSGF+G +T NP
Sbjct: 126 QPPPEGRLPDATKGCDHLRDVFAKQMGLSDKDIVALSGAHTLGRCHKDRSGFEGAWTSNP 185

Query: 185 LTFDNSYFVELLRGDTPGLVKFPTDKALLTDPRFRPFVDLYARDQRAFFRDYAESHKKMS 244
           L FDNSYF ELL G+  GL++  +DKALL DP FRP V+ YA D+ AFF DYAE+H K+S
Sbjct: 186 LIFDNSYFKELLSGEKEGLLQLVSDKALLDDPVFRPLVEKYAADEDAFFADYAEAHMKLS 245

Query: 245 LLG 246
            LG
Sbjct: 246 ELG 246

[Close]

BLAST of Spo08328.1 vs. TAIR (Arabidopsis)
Match: AT1G77490.1 (thylakoidal ascorbate peroxidase)

HSP 1 Score: 186.8 bits (473), Expect = 1.900e-47
Identity = 108/250 (43.20%), Postives = 143/250 (57.20%), Query Frame = 1


		  
Query: 35  PILLRLSFHDAVDYDAATK----RGGANGSVRLAQELNRTPNKGIETAVRFCEPIKRRHP 94
           PIL+RL +HDA  Y+   +    RGGANGS+R   EL    N G+  A++  +P+K ++P
Sbjct: 104 PILVRLGWHDAGTYNKNIEEWPLRGGANGSLRFEAELKHAANAGLLNALKLIQPLKDKYP 163

Query: 95  DITYADLYQLAGIVAVEVTGGPAIDFVPGRADADVADQ----DNIPN--PRRGADHLRTV 154
           +I+YADL+QLA   A+E  GGP I    GR D    +Q      +P+  P   ADHLR V
Sbjct: 164 NISYADLFQLASATAIEEAGGPDIPMKYGRVDVVAPEQCPEEGRLPDAGPPSPADHLRDV 223

Query: 155 FYRMGLNDKDIVVLSGAHALGGAHKDRSGFDGDFTRNP----------------LTFDNS 214
           FYRMGL+DK+IV LSGAH LG A  DRSG+    T+                  L FDNS
Sbjct: 224 FYRMGLDDKEIVALSGAHTLGRARPDRSGWGKPETKYTKTGPGEAGGQSWTVKWLKFDNS 283

Query: 215 YFVELLRGDTPGLVKFPTDKALLTDPRFRPFVDLYARDQRAFFRDYAESHKKMSLLG--L 257
           YF ++       L+  PTD AL  DP F+ + + YA D  AFF+DYAE+H K+S LG   
Sbjct: 284 YFKDIKEKRDDDLLVLPTDAALFEDPSFKNYAEKYAEDVAAFFKDYAEAHAKLSNLGAKF 343

The following BLAST results are available for this feature:

BLAST of Spo08328.1 vs. NCBI nr
BLAST of Spo08328.1 vs. UniProtKB/TrEMBL
BLAST of Spo08328.1 vs. ExPASy Swiss-Prot
BLAST of Spo08328.1 vs. TAIR (Arabidopsis)

BLAST of Spo08328.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902219780\|gb\|KNA18404.1\|	1.6e-173	100.	hypothetical protein SOVF_0708...	[more]
gi\|987701\|dbj\|BAA08535.1\|	9.7e-142	97.3	ascorbate peroxidase [Spinacia...	[more]
gi\|870858051\|gb\|KMT09577.1\|	2.8e-133	79.8	hypothetical protein BVRB_6g13...	[more]
gi\|870858054\|gb\|KMT09580.1\|	3.2e-100	80.3	hypothetical protein BVRB_6g13...	[more]
gi\|3377755\|gb\|AAC28103.1\|	1.7e-90	67.2	ascorbate peroxidase [Mesembry...	[more]

BLAST of Spo08328.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9RHH7_SPIOL	1.1e-173	100.	Uncharacterized protein OS=Spi...	[more]
Q41371_SPIOL	6.8e-142	97.3	Ascorbate peroxidase OS=Spinac...	[more]
A0A0J8C7N2_BETVU	2.0e-133	79.8	Uncharacterized protein OS=Bet...	[more]
A0A0J8C797_BETVU	2.2e-100	80.3	Uncharacterized protein OS=Bet...	[more]
O81604_MESCR	1.2e-90	67.2	Ascorbate peroxidase OS=Mesemb...	[more]

BLAST of Spo08328.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
APX5_ARATH	2.9e-85	63.3	L-ascorbate peroxidase 5, pero...	[more]
APX3_ORYSI	2.3e-82	59.5	Probable L-ascorbate peroxidas...	[more]
APX3_ORYSJ	5.1e-82	59.1	Probable L-ascorbate peroxidas...	[more]
APX3_ARATH	3.6e-80	55.0	L-ascorbate peroxidase 3, pero...	[more]
APX4_ORYSJ	1.2e-78	58.6	Probable L-ascorbate peroxidas...	[more]

BLAST of Spo08328.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 5


Match Name	E-value	Identity	Description
AT4G35970.1	1.6e-86	63.3	ascorbate peroxidase 5	[more]
AT4G35000.1	2.0e-81	55.0	ascorbate peroxidase 3	[more]
AT3G09640.1	1.1e-69	53.0	ascorbate peroxidase 2	[more]
AT1G07890.3	9.2e-66	52.2	ascorbate peroxidase 1	[more]
AT1G77490.1	1.9e-47	43.2	thylakoidal ascorbate peroxida...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002016	Haem peroxidase, plant/fungal/bacterial	PRINTS	PR00458	PEROXIDASE	coord: 154..169 score: 2.5E-24coord: 34..48 score: 2.5E-24coord: 92..109 score: 2.5E-24coord: 110..122 score: 2.5E-24coord: 178..193 score: 2.5
IPR002016	Haem peroxidase, plant/fungal/bacterial	PFAM	PF00141	peroxidase	coord: 24..226 score: 1.9
IPR002016	Haem peroxidase, plant/fungal/bacterial	PROFILE	PS50873	PEROXIDASE_4	coord: 15..269 score: 18
IPR002207	Plant ascorbate peroxidase	PRINTS	PR00459	ASPEROXIDASE	coord: 130..147 score: 1.4E-73coord: 110..122 score: 1.4E-73coord: 148..169 score: 1.4E-73coord: 91..109 score: 1.4E-73coord: 170..194 score: 1.4E-73coord: 11..31 score: 1.4E-73coord: 66..90 score: 1.4E-73coord: 196..221 score: 1.4
IPR010255	Haem peroxidase	unknown	SSF48113	Heme-dependent peroxidases	coord: 14..250 score: 3.24
IPR019793	Peroxidases heam-ligand binding site	PROSITE	PS00435	PEROXIDASE_1	coord: 154..164 scor
IPR019794	Peroxidase, active site	PROSITE	PS00436	PEROXIDASE_2	coord: 34..45 scor
None	No IPR available	GENE3D	1.10.420.10		coord: 151..187 score: 6.2E-21coord: 188..241 score: 2.3
None	No IPR available	GENE3D	1.10.520.10		coord: 13..150 score: 5.3
None	No IPR available	PANTHER	PTHR31356	FAMILY NOT NAMED	coord: 5..252 score: 6.2E
None	No IPR available	PANTHER	PTHR31356:SF8	L-ASCORBATE PEROXIDASE 5, PEROXISOMAL	coord: 5..252 score: 6.2E

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0006979	response to oxidative stress
biological_process	GO:0019852	L-ascorbic acid metabolic process
cellular_component	GO:0016020	membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0004601	peroxidase activity
molecular_function	GO:0016688	L-ascorbate peroxidase activity