Spo08341.1 (mRNA) | SpinachBase

Overview


Name	Spo08341.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	Calmodulin-like protein 6a
Location	chr3 : 11648095 .. 11648595 (+)
Sequence length	501

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTGTCCCACCGGTACTTCCCTACGTCCGTCGAGCATTACAGACGAACCCACGTCAGAATTCCGGCGAGCATTCGAAGTCATAGACTTCGACCACGACGGGAAAATCTCCGGCGAAGATCTCCGTTTATTCTACACATCATTTTCCGGCAGCGGCGACGACGACGAAATGATAAAGGCAATGATATCCGTAGCCGACGCGAATAAAGACGGGTACGTACAATACGACGAGTTTGAGAAGGTGTTGAAGGAGAGAAGAGTGAAAGGGATGAAGGAGAAAAGCTACGGTAACGGCGCGGTGATGGAAGAGGTGTTTAAGGTGATGGATAAAGATGGTGATGGAAGATTAGGGGTTAATGATTTGAGTGATTATCTAAGATTAGCTGGGATTGAGATTGCTAATGATGAGATTAAGGCTATGATTAAGTTGGGTTGTGGCGGTGGTGATGAGAAAGACGGTGTTTCTTTTGATGGATTTGTCAATATTTTTGGATCTCTATGA

mRNA sequence

ATGTGTCCCACCGGTACTTCCCTACGTCCGTCGAGCATTACAGACGAACCCACGTCAGAATTCCGGCGAGCATTCGAAGTCATAGACTTCGACCACGACGGGAAAATCTCCGGCGAAGATCTCCGTTTATTCTACACATCATTTTCCGGCAGCGGCGACGACGACGAAATGATAAAGGCAATGATATCCGTAGCCGACGCGAATAAAGACGGGTACGTACAATACGACGAGTTTGAGAAGGTGTTGAAGGAGAGAAGAGTGAAAGGGATGAAGGAGAAAAGCTACGGTAACGGCGCGGTGATGGAAGAGGTGTTTAAGGTGATGGATAAAGATGGTGATGGAAGATTAGGGGTTAATGATTTGAGTGATTATCTAAGATTAGCTGGGATTGAGATTGCTAATGATGAGATTAAGGCTATGATTAAGTTGGGTTGTGGCGGTGGTGATGAGAAAGACGGTGTTTCTTTTGATGGATTTGTCAATATTTTTGGATCTCTATGA

Coding sequence (CDS)

ATGTGTCCCACCGGTACTTCCCTACGTCCGTCGAGCATTACAGACGAACCCACGTCAGAATTCCGGCGAGCATTCGAAGTCATAGACTTCGACCACGACGGGAAAATCTCCGGCGAAGATCTCCGTTTATTCTACACATCATTTTCCGGCAGCGGCGACGACGACGAAATGATAAAGGCAATGATATCCGTAGCCGACGCGAATAAAGACGGGTACGTACAATACGACGAGTTTGAGAAGGTGTTGAAGGAGAGAAGAGTGAAAGGGATGAAGGAGAAAAGCTACGGTAACGGCGCGGTGATGGAAGAGGTGTTTAAGGTGATGGATAAAGATGGTGATGGAAGATTAGGGGTTAATGATTTGAGTGATTATCTAAGATTAGCTGGGATTGAGATTGCTAATGATGAGATTAAGGCTATGATTAAGTTGGGTTGTGGCGGTGGTGATGAGAAAGACGGTGTTTCTTTTGATGGATTTGTCAATATTTTTGGATCTCTATGA

Protein sequence

MCPTGTSLRPSSITDEPTSEFRRAFEVIDFDHDGKISGEDLRLFYTSFSGSGDDDEMIKAMISVADANKDGYVQYDEFEKVLKERRVKGMKEKSYGNGAVMEEVFKVMDKDGDGRLGVNDLSDYLRLAGIEIANDEIKAMIKLGCGGGDEKDGVSFDGFVNIFGSL

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo08341	Spo08341	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo08341.1	Spo08341.1-protein	polypeptide

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo08341.1.exon.1	Spo08341.1.exon.1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo08341.1.CDS.1	Spo08341.1.CDS.1	CDS

Homology

[Close]

BLAST of Spo08341.1 vs. NCBI nr
Match: gi|902220167|gb|KNA18508.1| (hypothetical protein SOVF_070110 [Spinacia oleracea])

HSP 1 Score: 332.8 bits (852), Expect = 3.400e-88
Identity = 166/166 (100.00%), Postives = 166/166 (100.00%), Query Frame = 1


		  
Query: 1   MCPTGTSLRPSSITDEPTSEFRRAFEVIDFDHDGKISGEDLRLFYTSFSGSGDDDEMIKA 60
           MCPTGTSLRPSSITDEPTSEFRRAFEVIDFDHDGKISGEDLRLFYTSFSGSGDDDEMIKA
Sbjct: 1   MCPTGTSLRPSSITDEPTSEFRRAFEVIDFDHDGKISGEDLRLFYTSFSGSGDDDEMIKA 60

Query: 61  MISVADANKDGYVQYDEFEKVLKERRVKGMKEKSYGNGAVMEEVFKVMDKDGDGRLGVND 120
           MISVADANKDGYVQYDEFEKVLKERRVKGMKEKSYGNGAVMEEVFKVMDKDGDGRLGVND
Sbjct: 61  MISVADANKDGYVQYDEFEKVLKERRVKGMKEKSYGNGAVMEEVFKVMDKDGDGRLGVND 120

Query: 121 LSDYLRLAGIEIANDEIKAMIKLGCGGGDEKDGVSFDGFVNIFGSL 167
           LSDYLRLAGIEIANDEIKAMIKLGCGGGDEKDGVSFDGFVNIFGSL
Sbjct: 121 LSDYLRLAGIEIANDEIKAMIKLGCGGGDEKDGVSFDGFVNIFGSL 166

[Close]

BLAST of Spo08341.1 vs. NCBI nr
Match: gi|731322190|ref|XP_010671757.1| (PREDICTED: calmodulin [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 246.9 bits (629), Expect = 2.500e-62
Identity = 121/162 (74.69%), Postives = 144/162 (88.89%), Query Frame = 1


		  
Query: 1   MCPTGTSLRPSSITDEPTSEFRRAFEVIDFDHDGKISGEDLRLFYTSFSGSGDDDEMIKA 60
           MCPTGTSL P++I+ E TS+FR+AFEVID DHDGKISGEDLR FY + SG+ DD+EMIK+
Sbjct: 1   MCPTGTSLPPTNISGEATSDFRQAFEVIDVDHDGKISGEDLRSFYAANSGTVDDEEMIKS 60

Query: 61  MISVADANKDGYVQYDEFEKVLKERRVKGMKEKSYGNGAVMEEVFKVMDKDGDGRLGVND 120
           MI VADANKDGYVQYDEFE+VL + R +  +EKSYG+G VMEE+FKVMDKDGDG+LGV+D
Sbjct: 61  MILVADANKDGYVQYDEFERVLNKGRTR--REKSYGDGGVMEEMFKVMDKDGDGKLGVDD 120

Query: 121 LSDYLRLAGIEIANDEIKAMIKLGCGGGDEKDGVSFDGFVNI 163
           L  YLRLAGIE+ +DEIKAMIKLGCGGGDE+DGVSF+GF++I
Sbjct: 121 LKSYLRLAGIEVVDDEIKAMIKLGCGGGDERDGVSFNGFLDI 160

[Close]

BLAST of Spo08341.1 vs. NCBI nr
Match: gi|743783482|ref|XP_011019795.1| (PREDICTED: calmodulin-like [Populus euphratica])

HSP 1 Score: 181.0 bits (458), Expect = 1.700e-42
Identity = 91/164 (55.49%), Postives = 122/164 (74.39%), Query Frame = 1


		  
Query: 1   MCPTGTSLRPSSITDEPTSEFRRAFEVIDFDHDGKISGEDLRLFYTSFSGSGDDDEMIKA 60
           MCP+GTSL   +   +  S+F+ AF+VID DHDGKIS +DLR+FY  FS +G DD+ I +
Sbjct: 1   MCPSGTSLAAGTKRGDARSDFKPAFDVIDADHDGKISKDDLRMFYAGFSSNGADDDFIGS 60

Query: 61  MISVADANKDGYVQYDEFEKVLKERRVKGMKEKSYGNGA-VMEEVFKVMDKDGDGRLGVN 120
           M+SVAD NKDG+VQYDEFE+VL     K     S  +G+ VME+VFKVMDKDGDG+L V+
Sbjct: 61  MMSVADFNKDGFVQYDEFERVLDGANEKKKSSSSTRSGSEVMEDVFKVMDKDGDGKLSVD 120

Query: 121 DLSDYLRLAGIEIANDEIKAMIKLGC-GGGDEKDGVSFDGFVNI 163
           DL  +++ AG + ++D+IKAMIKL C  GGD+KDGV+++ F+ I
Sbjct: 121 DLKSFMQWAGFDASDDDIKAMIKLACYSGGDDKDGVTYNDFLKI 164

[Close]

BLAST of Spo08341.1 vs. NCBI nr
Match: gi|567852401|ref|XP_006419364.1| (hypothetical protein CICLE_v10006076mg [Citrus clementina])

HSP 1 Score: 176.8 bits (447), Expect = 3.200e-41
Identity = 96/163 (58.90%), Postives = 113/163 (69.33%), Query Frame = 1


		  
Query: 1   MCPTGTSLRPSSITDEPTSEFRRAFEVIDFDHDGKISGEDLRLFYTSFSGSGDDDEMIKA 60
           MCPTGT+LRP        S  + AF+VID D DGKIS +DLR FY     S DDD++I  
Sbjct: 1   MCPTGTALRPRDAAKP--SRLKPAFDVIDADRDGKISRDDLRAFYAGRRASEDDDDVIGT 60

Query: 61  MISVADANKDGYVQYDEFEKVLK-ERRVKGMKEKSYGNGAVMEEVFKVMDKDGDGRLGVN 120
           MISVAD NKDGYV+YDEFE+VL  E      K KS G G +ME+VFKVMDKDGDGRL  +
Sbjct: 61  MISVADFNKDGYVEYDEFERVLDLEYGKANSKSKSNGGGGLMEDVFKVMDKDGDGRLSHD 120

Query: 121 DLSDYLRLAGIEIANDEIKAMIKLGCGGGDEKDGVSFDGFVNI 163
           DL  Y++ AG    +D+IKAMI+L  GGGDE DGVSFDG + I
Sbjct: 121 DLKSYMKCAGFAATDDDIKAMIRL--GGGDETDGVSFDGLLKI 159

[Close]

BLAST of Spo08341.1 vs. NCBI nr
Match: gi|224101537|ref|XP_002312322.1| (hypersensitive reaction associated Ca2+-binding family protein [Populus trichocarpa])

HSP 1 Score: 176.0 bits (445), Expect = 5.400e-41
Identity = 90/164 (54.88%), Postives = 117/164 (71.34%), Query Frame = 1


		  
Query: 1   MCPTGTSLRPSSITDEPTSEFRRAFEVIDFDHDGKISGEDLRLFYTSFSGSGDDDEMIKA 60
           MCP GTSL   +      S+FR AF+V+D DHDGKIS +DLR+FY  FS +G DD+ I +
Sbjct: 1   MCPYGTSLAAGTKRGGARSDFRPAFDVLDADHDGKISKDDLRMFYAGFSSNGADDDFIGS 60

Query: 61  MISVADANKDGYVQYDEFEKVLKERRVKGMKEKS-YGNGAVMEEVFKVMDKDGDGRLGVN 120
           M+SVAD NKDG+V+YDEFE+VL     K     S   +  VME+VFKVMDKDGDG+L V+
Sbjct: 61  MMSVADFNKDGFVEYDEFERVLDGASEKKKSSSSTRSSSGVMEDVFKVMDKDGDGKLSVD 120

Query: 121 DLSDYLRLAGIEIANDEIKAMIKLGC-GGGDEKDGVSFDGFVNI 163
           DL  Y++ AG + ++D+IKAMIKL C  GG +KDGV++D F+ I
Sbjct: 121 DLKSYMQWAGFDASDDDIKAMIKLACYSGGGDKDGVTYDDFLKI 164

[Close]

BLAST of Spo08341.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RG92_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_070110 PE=4 SV=1)

HSP 1 Score: 332.8 bits (852), Expect = 2.400e-88
Identity = 166/166 (100.00%), Postives = 166/166 (100.00%), Query Frame = 1


		  
Query: 1   MCPTGTSLRPSSITDEPTSEFRRAFEVIDFDHDGKISGEDLRLFYTSFSGSGDDDEMIKA 60
           MCPTGTSLRPSSITDEPTSEFRRAFEVIDFDHDGKISGEDLRLFYTSFSGSGDDDEMIKA
Sbjct: 1   MCPTGTSLRPSSITDEPTSEFRRAFEVIDFDHDGKISGEDLRLFYTSFSGSGDDDEMIKA 60

Query: 61  MISVADANKDGYVQYDEFEKVLKERRVKGMKEKSYGNGAVMEEVFKVMDKDGDGRLGVND 120
           MISVADANKDGYVQYDEFEKVLKERRVKGMKEKSYGNGAVMEEVFKVMDKDGDGRLGVND
Sbjct: 61  MISVADANKDGYVQYDEFEKVLKERRVKGMKEKSYGNGAVMEEVFKVMDKDGDGRLGVND 120

Query: 121 LSDYLRLAGIEIANDEIKAMIKLGCGGGDEKDGVSFDGFVNIFGSL 167
           LSDYLRLAGIEIANDEIKAMIKLGCGGGDEKDGVSFDGFVNIFGSL
Sbjct: 121 LSDYLRLAGIEIANDEIKAMIKLGCGGGDEKDGVSFDGFVNIFGSL 166

[Close]

BLAST of Spo08341.1 vs. UniProtKB/TrEMBL
Match: A0A0J8CWF2_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_3g054380 PE=4 SV=1)

HSP 1 Score: 246.9 bits (629), Expect = 1.700e-62
Identity = 121/162 (74.69%), Postives = 144/162 (88.89%), Query Frame = 1


		  
Query: 1   MCPTGTSLRPSSITDEPTSEFRRAFEVIDFDHDGKISGEDLRLFYTSFSGSGDDDEMIKA 60
           MCPTGTSL P++I+ E TS+FR+AFEVID DHDGKISGEDLR FY + SG+ DD+EMIK+
Sbjct: 1   MCPTGTSLPPTNISGEATSDFRQAFEVIDVDHDGKISGEDLRSFYAANSGTVDDEEMIKS 60

Query: 61  MISVADANKDGYVQYDEFEKVLKERRVKGMKEKSYGNGAVMEEVFKVMDKDGDGRLGVND 120
           MI VADANKDGYVQYDEFE+VL + R +  +EKSYG+G VMEE+FKVMDKDGDG+LGV+D
Sbjct: 61  MILVADANKDGYVQYDEFERVLNKGRTR--REKSYGDGGVMEEMFKVMDKDGDGKLGVDD 120

Query: 121 LSDYLRLAGIEIANDEIKAMIKLGCGGGDEKDGVSFDGFVNI 163
           L  YLRLAGIE+ +DEIKAMIKLGCGGGDE+DGVSF+GF++I
Sbjct: 121 LKSYLRLAGIEVVDDEIKAMIKLGCGGGDERDGVSFNGFLDI 160

[Close]

BLAST of Spo08341.1 vs. UniProtKB/TrEMBL
Match: V4U007_9ROSI (Uncharacterized protein OS=Citrus clementina GN=CICLE_v10006076mg PE=4 SV=1)

HSP 1 Score: 176.8 bits (447), Expect = 2.200e-41
Identity = 96/163 (58.90%), Postives = 113/163 (69.33%), Query Frame = 1


		  
Query: 1   MCPTGTSLRPSSITDEPTSEFRRAFEVIDFDHDGKISGEDLRLFYTSFSGSGDDDEMIKA 60
           MCPTGT+LRP        S  + AF+VID D DGKIS +DLR FY     S DDD++I  
Sbjct: 1   MCPTGTALRPRDAAKP--SRLKPAFDVIDADRDGKISRDDLRAFYAGRRASEDDDDVIGT 60

Query: 61  MISVADANKDGYVQYDEFEKVLK-ERRVKGMKEKSYGNGAVMEEVFKVMDKDGDGRLGVN 120
           MISVAD NKDGYV+YDEFE+VL  E      K KS G G +ME+VFKVMDKDGDGRL  +
Sbjct: 61  MISVADFNKDGYVEYDEFERVLDLEYGKANSKSKSNGGGGLMEDVFKVMDKDGDGRLSHD 120

Query: 121 DLSDYLRLAGIEIANDEIKAMIKLGCGGGDEKDGVSFDGFVNI 163
           DL  Y++ AG    +D+IKAMI+L  GGGDE DGVSFDG + I
Sbjct: 121 DLKSYMKCAGFAATDDDIKAMIRL--GGGDETDGVSFDGLLKI 159

[Close]

BLAST of Spo08341.1 vs. UniProtKB/TrEMBL
Match: B9HIM5_POPTR (Hypersensitive reaction associated Ca2+-binding family protein OS=Populus trichocarpa GN=POPTR_0008s10360g PE=4 SV=1)

HSP 1 Score: 176.0 bits (445), Expect = 3.700e-41
Identity = 90/164 (54.88%), Postives = 117/164 (71.34%), Query Frame = 1


		  
Query: 1   MCPTGTSLRPSSITDEPTSEFRRAFEVIDFDHDGKISGEDLRLFYTSFSGSGDDDEMIKA 60
           MCP GTSL   +      S+FR AF+V+D DHDGKIS +DLR+FY  FS +G DD+ I +
Sbjct: 1   MCPYGTSLAAGTKRGGARSDFRPAFDVLDADHDGKISKDDLRMFYAGFSSNGADDDFIGS 60

Query: 61  MISVADANKDGYVQYDEFEKVLKERRVKGMKEKS-YGNGAVMEEVFKVMDKDGDGRLGVN 120
           M+SVAD NKDG+V+YDEFE+VL     K     S   +  VME+VFKVMDKDGDG+L V+
Sbjct: 61  MMSVADFNKDGFVEYDEFERVLDGASEKKKSSSSTRSSSGVMEDVFKVMDKDGDGKLSVD 120

Query: 121 DLSDYLRLAGIEIANDEIKAMIKLGC-GGGDEKDGVSFDGFVNI 163
           DL  Y++ AG + ++D+IKAMIKL C  GG +KDGV++D F+ I
Sbjct: 121 DLKSYMQWAGFDASDDDIKAMIKLACYSGGGDKDGVTYDDFLKI 164

[Close]

BLAST of Spo08341.1 vs. UniProtKB/TrEMBL
Match: A0A067G974_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g031051mg PE=4 SV=1)

HSP 1 Score: 171.8 bits (434), Expect = 7.100e-40
Identity = 94/163 (57.67%), Postives = 110/163 (67.48%), Query Frame = 1


		  
Query: 1   MCPTGTSLRPSSITDEPTSEFRRAFEVIDFDHDGKISGEDLRLFYTSFSGSGDDDEMIKA 60
           MCPTGT+LRP        S  + AF+VID D DGKIS +DLR FY     S +D ++I  
Sbjct: 1   MCPTGTALRPRDAAKP--SRLKPAFDVIDADRDGKISRDDLRAFYAGRRASEEDGDVIGT 60

Query: 61  MISVADANKDGYVQYDEFEKVLK-ERRVKGMKEKSYGNGAVMEEVFKVMDKDGDGRLGVN 120
           MISVAD NKDGYV+YDEFE+VL  E      K KS G G +ME+VFKVMDKDGDGRL   
Sbjct: 61  MISVADFNKDGYVEYDEFERVLDLEYGKANSKSKSNGGGGLMEDVFKVMDKDGDGRLSHG 120

Query: 121 DLSDYLRLAGIEIANDEIKAMIKLGCGGGDEKDGVSFDGFVNI 163
           DL  Y+  AG    +D+IKAMI+L  GGGDE DGVSFDG + I
Sbjct: 121 DLKSYMNCAGFAATDDDIKAMIRL--GGGDENDGVSFDGLLKI 159

[Close]

BLAST of Spo08341.1 vs. ExPASy Swiss-Prot
Match: CALM_SCHPO (Calmodulin OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) GN=cam1 PE=1 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 4.400e-11
Identity = 47/154 (30.52%), Postives = 78/154 (50.65%), Query Frame = 1


		  
Query: 12  SITDEPTSEFRRAFEVIDFDHDGKISGEDLRLFYTSFSGSGDDDEMIKAMISVADANKDG 71
           ++TDE  +EFR AF + D D DG I+  +L +   S   S    E+ + MI+  DA+ +G
Sbjct: 5   NLTDEQIAEFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAEL-QDMINEVDADGNG 64

Query: 72  YVQYDEFEKVLKERRVKGMKEKSYGNGAVMEEVFKVMDKDGDGRLGVNDLSDYLRLAGIE 131
            + + EF  ++        K K   N   + E FKV DKDG+G + V +L+  L   G  
Sbjct: 65  TIDFTEFLTMMAR------KMKDTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGER 124

Query: 132 IANDEIKAMIKLGCGGGDEKDGVSFDGFVNIFGS 166
           ++ +E+  MI+     GD    ++++ F  +  S
Sbjct: 125 LSQEEVADMIREADTDGD--GVINYEEFSRVISS 149

[Close]

BLAST of Spo08341.1 vs. ExPASy Swiss-Prot
Match: CALM_COLTR (Calmodulin OS=Colletotrichum trifolii PE=3 SV=2)

HSP 1 Score: 67.4 bits (163), Expect = 1.700e-10
Identity = 42/151 (27.81%), Postives = 77/151 (50.99%), Query Frame = 1


		  
Query: 12  SITDEPTSEFRRAFEVIDFDHDGKISGEDLRLFYTSFSGSGDDDEMIKAMISVADANKDG 71
           S+T+E  SEF+ AF + D D DG+I+ ++L     S  G    +  ++ MI+  DA+ +G
Sbjct: 4   SLTEEQVSEFKEAFSLFDKDGDGQITTKELGTVMRSL-GQNPSESELQDMINEVDADNNG 63

Query: 72  YVQYDEFEKVLKERRVKGMKEKSYGNGAVMEEVFKVMDKDGDGRLGVNDLSDYLRLAGIE 131
            + + EF  ++        K K   +   + E FKV D+D +G +   +L   +   G +
Sbjct: 64  TIDFPEFLTMM------ARKMKDTDSEEEIREAFKVFDRDNNGFISAAELRHVMTSIGEK 123

Query: 132 IANDEIKAMIKLGCGGGDEKDGVSFDGFVNI 163
           + +DE+  MI+     GD +  + ++ FV +
Sbjct: 124 LTDDEVDEMIREADQDGDGR--IDYNEFVQL 145

[Close]

BLAST of Spo08341.1 vs. ExPASy Swiss-Prot
Match: CALM_COLGL (Calmodulin OS=Colletotrichum gloeosporioides PE=2 SV=2)

HSP 1 Score: 67.4 bits (163), Expect = 1.700e-10
Identity = 42/151 (27.81%), Postives = 77/151 (50.99%), Query Frame = 1


		  
Query: 12  SITDEPTSEFRRAFEVIDFDHDGKISGEDLRLFYTSFSGSGDDDEMIKAMISVADANKDG 71
           S+T+E  SEF+ AF + D D DG+I+ ++L     S  G    +  ++ MI+  DA+ +G
Sbjct: 4   SLTEEQVSEFKEAFSLFDKDGDGQITTKELGTVMRSL-GQNPSESELQDMINEVDADNNG 63

Query: 72  YVQYDEFEKVLKERRVKGMKEKSYGNGAVMEEVFKVMDKDGDGRLGVNDLSDYLRLAGIE 131
            + + EF  ++        K K   +   + E FKV D+D +G +   +L   +   G +
Sbjct: 64  TIDFPEFLTMM------ARKMKDTDSEEEIREAFKVFDRDNNGFISAAELRHVMTSIGEK 123

Query: 132 IANDEIKAMIKLGCGGGDEKDGVSFDGFVNI 163
           + +DE+  MI+     GD +  + ++ FV +
Sbjct: 124 LTDDEVDEMIREADQDGDGR--IDYNEFVQL 145

[Close]

BLAST of Spo08341.1 vs. ExPASy Swiss-Prot
Match: CALM_NEUCR (Calmodulin OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) GN=cmd-1 PE=1 SV=2)

HSP 1 Score: 67.4 bits (163), Expect = 1.700e-10
Identity = 42/151 (27.81%), Postives = 77/151 (50.99%), Query Frame = 1


		  
Query: 12  SITDEPTSEFRRAFEVIDFDHDGKISGEDLRLFYTSFSGSGDDDEMIKAMISVADANKDG 71
           S+T+E  SEF+ AF + D D DG+I+ ++L     S  G    +  ++ MI+  DA+ +G
Sbjct: 4   SLTEEQVSEFKEAFSLFDKDGDGQITTKELGTVMRSL-GQNPSESELQDMINEVDADNNG 63

Query: 72  YVQYDEFEKVLKERRVKGMKEKSYGNGAVMEEVFKVMDKDGDGRLGVNDLSDYLRLAGIE 131
            + + EF  ++        K K   +   + E FKV D+D +G +   +L   +   G +
Sbjct: 64  TIDFPEFLTMM------ARKMKDTDSEEEIREAFKVFDRDNNGFISAAELRHVMTSIGEK 123

Query: 132 IANDEIKAMIKLGCGGGDEKDGVSFDGFVNI 163
           + +DE+  MI+     GD +  + ++ FV +
Sbjct: 124 LTDDEVDEMIREADQDGDGR--IDYNEFVQL 145

[Close]

BLAST of Spo08341.1 vs. ExPASy Swiss-Prot
Match: CALM_MAGO7 (Calmodulin OS=Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) GN=CMD1 PE=3 SV=4)

HSP 1 Score: 67.4 bits (163), Expect = 1.700e-10
Identity = 42/151 (27.81%), Postives = 77/151 (50.99%), Query Frame = 1


		  
Query: 12  SITDEPTSEFRRAFEVIDFDHDGKISGEDLRLFYTSFSGSGDDDEMIKAMISVADANKDG 71
           S+T+E  SEF+ AF + D D DG+I+ ++L     S  G    +  ++ MI+  DA+ +G
Sbjct: 4   SLTEEQVSEFKEAFSLFDKDGDGQITTKELGTVMRSL-GQNPSESELQDMINEVDADNNG 63

Query: 72  YVQYDEFEKVLKERRVKGMKEKSYGNGAVMEEVFKVMDKDGDGRLGVNDLSDYLRLAGIE 131
            + + EF  ++        K K   +   + E FKV D+D +G +   +L   +   G +
Sbjct: 64  TIDFPEFLTMM------ARKMKDTDSEEEIREAFKVFDRDNNGFISAAELRHVMTSIGEK 123

Query: 132 IANDEIKAMIKLGCGGGDEKDGVSFDGFVNI 163
           + +DE+  MI+     GD +  + ++ FV +
Sbjct: 124 LTDDEVDEMIREADQDGDGR--IDYNEFVQL 145

[Close]

BLAST of Spo08341.1 vs. TAIR (Arabidopsis)
Match: AT5G49480.1 (Ca2+-binding protein 1)

HSP 1 Score: 165.6 bits (418), Expect = 2.600e-41
Identity = 90/165 (54.55%), Postives = 113/165 (68.48%), Query Frame = 1


		  
Query: 1   MCPTGTSLRPSSITDEPTSEFRRAFEVIDFDHDGKISGEDLRLFYTSF-SGSGDDDEMIK 60
           MCP+G    P + T  P   FR AFE+ID DHDGKIS +DLR FY    SG  +D+ MI 
Sbjct: 1   MCPSGRIAIPITTTANPN--FRPAFEIIDTDHDGKISSDDLRAFYAGIPSGENNDETMIG 60

Query: 61  AMISVADANKDGYVQYDEFEKVLKERRVKGMKEKSYGNGAVMEEVFKVMDKDGDGRLGVN 120
            MISVADANKDG+V++DEFEKVL+       +  + G+  +M++VFKVMDKDGDGRL   
Sbjct: 61  TMISVADANKDGFVEFDEFEKVLETTPFS--RSGNGGDDGLMKDVFKVMDKDGDGRLSYG 120

Query: 121 DLSDYLRLAGIEIANDEIKAMIKLGCGGGDEKDGVSFDGFVNIFG 165
           DL  Y+  AG+ + +DEIK+MI+L   GGD  DGVSFDG + IFG
Sbjct: 121 DLKSYMDSAGLAVTDDEIKSMIRL--AGGDLNDGVSFDGLLKIFG 159

[Close]

BLAST of Spo08341.1 vs. TAIR (Arabidopsis)
Match: AT3G56800.1 (calmodulin 3)

HSP 1 Score: 62.8 bits (151), Expect = 2.300e-10
Identity = 41/150 (27.33%), Postives = 77/150 (51.33%), Query Frame = 1


		  
Query: 13  ITDEPTSEFRRAFEVIDFDHDGKISGEDLRLFYTSFSGSGDDDEMIKAMISVADANKDGY 72
           +TD+  SEF+ AF + D D DG I+ ++L     S  G    +  ++ MI+  DA+ +G 
Sbjct: 5   LTDDQISEFKEAFSLFDKDGDGCITTKELGTVMRSL-GQNPTEAELQDMINEVDADGNGT 64

Query: 73  VQYDEFEKVLKERRVKGMKEKSYGNGAVMEEVFKVMDKDGDGRLGVNDLSDYLRLAGIEI 132
           + + EF  ++        K K   +   ++E F+V DKD +G +   +L   +   G ++
Sbjct: 65  IDFPEFLNLM------ARKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKL 124

Query: 133 ANDEIKAMIKLGCGGGDEKDGVSFDGFVNI 163
            ++E+  MIK     GD +  ++++ FV +
Sbjct: 125 TDEEVDEMIKEADVDGDGQ--INYEEFVKV 145

[Close]

BLAST of Spo08341.1 vs. TAIR (Arabidopsis)
Match: AT2G27030.3 (calmodulin 5)

HSP 1 Score: 62.8 bits (151), Expect = 2.300e-10
Identity = 41/150 (27.33%), Postives = 77/150 (51.33%), Query Frame = 1


		  
Query: 13  ITDEPTSEFRRAFEVIDFDHDGKISGEDLRLFYTSFSGSGDDDEMIKAMISVADANKDGY 72
           +TD+  SEF+ AF + D D DG I+ ++L     S  G    +  ++ MI+  DA+ +G 
Sbjct: 5   LTDDQISEFKEAFSLFDKDGDGCITTKELGTVMRSL-GQNPTEAELQDMINEVDADGNGT 64

Query: 73  VQYDEFEKVLKERRVKGMKEKSYGNGAVMEEVFKVMDKDGDGRLGVNDLSDYLRLAGIEI 132
           + + EF  ++        K K   +   ++E F+V DKD +G +   +L   +   G ++
Sbjct: 65  IDFPEFLNLM------ARKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKL 124

Query: 133 ANDEIKAMIKLGCGGGDEKDGVSFDGFVNI 163
            ++E+  MIK     GD +  ++++ FV +
Sbjct: 125 TDEEVDEMIKEADVDGDGQ--INYEEFVKV 145

[Close]

BLAST of Spo08341.1 vs. TAIR (Arabidopsis)
Match: AT5G21274.1 (calmodulin 6)

HSP 1 Score: 62.0 bits (149), Expect = 4.000e-10
Identity = 40/150 (26.67%), Postives = 78/150 (52.00%), Query Frame = 1


		  
Query: 13  ITDEPTSEFRRAFEVIDFDHDGKISGEDLRLFYTSFSGSGDDDEMIKAMISVADANKDGY 72
           +TD+  SEF+ AF + D D DG I+ ++L     S  G    +  ++ MI+  DA+ +G 
Sbjct: 5   LTDDQISEFKEAFSLFDKDGDGCITTKELGTVMRSL-GQNPTEAELQDMINEVDADGNGT 64

Query: 73  VQYDEFEKVLKERRVKGMKEKSYGNGAVMEEVFKVMDKDGDGRLGVNDLSDYLRLAGIEI 132
           + + EF  ++        K K   +   ++E F+V DKD +G +   +L   +   G ++
Sbjct: 65  IDFPEFLNLM------ARKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKL 124

Query: 133 ANDEIKAMIKLGCGGGDEKDGVSFDGFVNI 163
           +++E+  MI+     GD +  ++++ FV +
Sbjct: 125 SDEEVDEMIREADVDGDGQ--INYEEFVKV 145

[Close]

BLAST of Spo08341.1 vs. TAIR (Arabidopsis)
Match: AT3G43810.1 (calmodulin 7)

HSP 1 Score: 61.6 bits (148), Expect = 5.200e-10
Identity = 40/150 (26.67%), Postives = 77/150 (51.33%), Query Frame = 1


		  
Query: 13  ITDEPTSEFRRAFEVIDFDHDGKISGEDLRLFYTSFSGSGDDDEMIKAMISVADANKDGY 72
           +TD+  SEF+ AF + D D DG I+ ++L     S  G    +  ++ MI+  DA+ +G 
Sbjct: 5   LTDDQISEFKEAFSLFDKDGDGCITTKELGTVMRSL-GQNPTEAELQDMINEVDADGNGT 64

Query: 73  VQYDEFEKVLKERRVKGMKEKSYGNGAVMEEVFKVMDKDGDGRLGVNDLSDYLRLAGIEI 132
           + + EF  ++        K K   +   ++E F+V DKD +G +   +L   +   G ++
Sbjct: 65  IDFPEFLNLM------ARKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKL 124

Query: 133 ANDEIKAMIKLGCGGGDEKDGVSFDGFVNI 163
            ++E+  MI+     GD +  ++++ FV +
Sbjct: 125 TDEEVDEMIREADVDGDGQ--INYEEFVKV 145

The following BLAST results are available for this feature:

BLAST of Spo08341.1 vs. NCBI nr
BLAST of Spo08341.1 vs. UniProtKB/TrEMBL
BLAST of Spo08341.1 vs. ExPASy Swiss-Prot
BLAST of Spo08341.1 vs. TAIR (Arabidopsis)

BLAST of Spo08341.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902220167\|gb\|KNA18508.1\|	3.4e-88	100.	hypothetical protein SOVF_0701...	[more]
gi\|731322190\|ref\|XP_010671757.1\|	2.5e-62	74.6	PREDICTED: calmodulin [Beta vu...	[more]
gi\|743783482\|ref\|XP_011019795.1\|	1.7e-42	55.4	PREDICTED: calmodulin-like [Po...	[more]
gi\|567852401\|ref\|XP_006419364.1\|	3.2e-41	58.9	hypothetical protein CICLE_v10...	[more]
gi\|224101537\|ref\|XP_002312322.1\|	5.4e-41	54.8	hypersensitive reaction associ...	[more]

BLAST of Spo08341.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9RG92_SPIOL	2.4e-88	100.	Uncharacterized protein OS=Spi...	[more]
A0A0J8CWF2_BETVU	1.7e-62	74.6	Uncharacterized protein OS=Bet...	[more]
V4U007_9ROSI	2.2e-41	58.9	Uncharacterized protein OS=Cit...	[more]
B9HIM5_POPTR	3.7e-41	54.8	Hypersensitive reaction associ...	[more]
A0A067G974_CITSI	7.1e-40	57.6	Uncharacterized protein OS=Cit...	[more]

BLAST of Spo08341.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
CALM_SCHPO	4.4e-11	30.5	Calmodulin OS=Schizosaccharomy...	[more]
CALM_COLTR	1.7e-10	27.8	Calmodulin OS=Colletotrichum t...	[more]
CALM_COLGL	1.7e-10	27.8	Calmodulin OS=Colletotrichum g...	[more]
CALM_NEUCR	1.7e-10	27.8	Calmodulin OS=Neurospora crass...	[more]
CALM_MAGO7	1.7e-10	27.8	Calmodulin OS=Magnaporthe oryz...	[more]

BLAST of Spo08341.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 5


Match Name	E-value	Identity	Description
AT5G49480.1	2.6e-41	54.5	Ca2+-binding protein 1	[more]
AT3G56800.1	2.3e-10	27.3	calmodulin 3	[more]
AT2G27030.3	2.3e-10	27.3	calmodulin 5	[more]
AT5G21274.1	4.0e-10	26.6	calmodulin 6	[more]
AT3G43810.1	5.2e-10	26.6	calmodulin 7	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	PFAM	PF13405	EF-hand_6	coord: 101..127 score: 3.
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 20..81 score: 4.9
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 20..48 score: 7.7E-4coord: 57..85 score: 9.2E-4coord: 100..128 score:
IPR002048	EF-hand domain	PROFILE	PS50222	EF_HAND_2	coord: 96..131 score: 12.979coord: 16..51 score: 12.672coord: 53..88 score: 12
IPR011992	EF-hand domain pair	GENE3D	1.10.238.10		coord: 12..163 score: 6.8
IPR011992	EF-hand domain pair	unknown	SSF47473	EF-hand	coord: 9..163 score: 4.06
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 66..78 score: -coord: 109..121 score: -coord: 29..41 scor
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 19..163 score: 1.9
None	No IPR available	PANTHER	PTHR10891:SF629	SUBFAMILY NOT NAMED	coord: 19..163 score: 1.9

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009740	gibberellic acid mediated signaling pathway
biological_process	GO:0009686	gibberellin biosynthetic process
biological_process	GO:0042538	hyperosmotic salinity response
biological_process	GO:0010114	response to red light
biological_process	GO:0009845	seed germination
cellular_component	GO:0005618	cell wall
cellular_component	GO:0005829	cytosol
cellular_component	GO:0005777	peroxisome
molecular_function	GO:0005509	calcium ion binding