Spo08546.1 (mRNA)

Overview
NameSpo08546.1
TypemRNA
OrganismSpinacia oleracea (Spinach)
DescriptionSplicing factor PWI domain-containing protein / RNA recognition motif-containing protein isoform 2
LocationSuper_scaffold_51 : 418008 .. 419509 (+)
Sequence length642
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTAAATTACGGTCTTTTTTCATCAAATGTCTTGGGTTTAATTGAGATGGTGAAAATTTTGTTATACTCATTAGCGAAACATGTTAGTTGGTGCGTTGGCTATTTTGGCAAGGCTACTATTGTTGCATCTGGATATATTACGAGGTAAGCATGCTTGGTGACTTGTTGATTATGAACCTTGTATTATTTTGTTGATCTCCATCAGGTCTGATCAGCAGCTCATAGTTTCCCTGCTATGATGCCATGTCTCTTATTTGTGATGTGTGGTAACAACACTTTTAAAGATTGTGAAGGTTTCTTTGCCCCTATTTATGAAGTGATATTTTTTATTATTTTATACGTTAATTACAAACCCATGCTGATGTGTTTACATAGATGTTTTTTACATGATTGTGCAGGAGTTGTTCGTTATCCTTATCCTCCAATGGCTCGTCCTCCTCTCCTTCCTCGACCTGGAGTTACAGTTGGCATGGTTCCGCAAATGCCAGGCCTCCTTTTTTGGGACATCGTCCTCCAATGACTCTCCCAATTGTTAGGCCCCCTGTTGTTCCAGTTGTTATGCCAGCAGAGAAACCACAAACTACTGTTTATGTTGGCAAGATAGCCTCTTCGGTGGAGAGTGATTTCGTACGTTCACTCCTTGAGGTATGTTAATATCATGAAGGACTTTTTTTTATTTTGTTTCTTATTCCTTCCCATGCTGGCATGGTATATATTTTCCTTTTCATATTTTTTGTCCCATTCTTTATACTGTGAAACTTTTTATTTTACTTAGTTATGCGGACCAATAAAGAGTTGGAAGCGATCTCAGGATCTGTCGGATGCAACACCAAGAAGATTTGGTTTCTGTGAATTTGAATCTGCTGAAGGGGTTCTCCGAGCTCTACAACTGCTGAGTAAATGCATTAGAGCTTCCTTCTGTTCCAGTTGCTAACGGAAGTGAGACACCTGCCTCACTCTCACCTGAATTTACTGCAAGTGAGACTAAAGATGATATGGAACACGATCATGATTCTGCTCCTGCTCTTGCTTCTGCTCCTGTTTCGGTTCCTTCTCTTGCTCCTGATCCTGCAAGCCATACTAGTAAGTGGCTATATAATTATTAGTCTTCTTTTTTCCTTCTACTTCTGCTTCTTATTATTATTGATTTATTTGGGTTTTTCATACAATTTACTTCTAAAACTAGTAATTTCAGGAAGTTGTATTTTGGAGTTTTATTGAATTGATCTATTAATTGTTAACTCGTACCGGAAAAGTGATATTTGTATCTTTATTGCTCTAAATTCGAGTTCATTAAACTTAATTGTTCCTGTATTCATTCATATTTGCGACACAAAAACATACACCAATTTTTGTGGATGAGTATTTTTATATCACGGATTTATTTTTGGTTGATGGGGACTAGAGAATACAGATACATCCCATACAGGTAACCTGGATTTTTTTTATGAGTTAACTATTTGATCATCTTTAATTTCAGGCCTTTTGTATGTCTCGTGA

mRNA sequence

ATGCTAAATTACGGTCTTTTTTCATCAAATGTCTTGGGTTTAATTGAGATGGTGAAAATTTTGTTATACTCATTAGCGAAACATGTTAGTTGGTGCGTTGGCTATTTTGGCAAGGCTACTATTGTTGCATCTGGATATATTACGAGGAGTTGTTCGTTATCCTTATCCTCCAATGGCTCGTCCTCCTCTCCTTCCTCGACCTGGAGTTACAGTTGGCATGGTTCCGCAAATGCCAGGCCTCCTTTTTTGGGACATCGTCCTCCAATGACTCTCCCAATTGTTAGGCCCCCTGTTGTTCCAGTTGTTATGCCAGCAGAGAAACCACAAACTACTGTTTATGTTGGCAAGATAGCCTCTTCGGTGGAGAGTGATTTCGTACGTTCACTCCTTGAGTTATGCGGACCAATAAAGAGTTGGAAGCGATCTCAGGATCTGTCGGATGCAACACCAAGAAGATTTGGTTTCTTTGCTAACGGAAGTGAGACACCTGCCTCACTCTCACCTGAATTTACTGCAAGTGAGACTAAAGATGATATGGAACACGATCATGATTCTGCTCCTGCTCTTGCTTCTGCTCCTGTTTCGGTTCCTTCTCTTGCTCCTGATCCTGCAAGCCATACTAGCCTTTTGTATGTCTCGTGA

Coding sequence (CDS)

ATGCTAAATTACGGTCTTTTTTCATCAAATGTCTTGGGTTTAATTGAGATGGTGAAAATTTTGTTATACTCATTAGCGAAACATGTTAGTTGGTGCGTTGGCTATTTTGGCAAGGCTACTATTGTTGCATCTGGATATATTACGAGGAGTTGTTCGTTATCCTTATCCTCCAATGGCTCGTCCTCCTCTCCTTCCTCGACCTGGAGTTACAGTTGGCATGGTTCCGCAAATGCCAGGCCTCCTTTTTTGGGACATCGTCCTCCAATGACTCTCCCAATTGTTAGGCCCCCTGTTGTTCCAGTTGTTATGCCAGCAGAGAAACCACAAACTACTGTTTATGTTGGCAAGATAGCCTCTTCGGTGGAGAGTGATTTCGTACGTTCACTCCTTGAGTTATGCGGACCAATAAAGAGTTGGAAGCGATCTCAGGATCTGTCGGATGCAACACCAAGAAGATTTGGTTTCTTTGCTAACGGAAGTGAGACACCTGCCTCACTCTCACCTGAATTTACTGCAAGTGAGACTAAAGATGATATGGAACACGATCATGATTCTGCTCCTGCTCTTGCTTCTGCTCCTGTTTCGGTTCCTTCTCTTGCTCCTGATCCTGCAAGCCATACTAGCCTTTTGTATGTCTCGTGA

Protein sequence

MLNYGLFSSNVLGLIEMVKILLYSLAKHVSWCVGYFGKATIVASGYITRSCSLSLSSNGSSSSPSSTWSYSWHGSANARPPFLGHRPPMTLPIVRPPVVPVVMPAEKPQTTVYVGKIASSVESDFVRSLLELCGPIKSWKRSQDLSDATPRRFGFFANGSETPASLSPEFTASETKDDMEHDHDSAPALASAPVSVPSLAPDPASHTSLLYVS
Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Spo08546Spo08546gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Spo08546.1Spo08546.1-proteinpolypeptide


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Spo08546.1.exon.1Spo08546.1.exon.1exon
Spo08546.1.exon.2Spo08546.1.exon.2exon
Spo08546.1.exon.3Spo08546.1.exon.3exon
Spo08546.1.exon.4Spo08546.1.exon.4exon
Spo08546.1.exon.5Spo08546.1.exon.5exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Spo08546.1.CDS.1Spo08546.1.CDS.1CDS
Spo08546.1.CDS.2Spo08546.1.CDS.2CDS
Spo08546.1.CDS.3Spo08546.1.CDS.3CDS
Spo08546.1.CDS.4Spo08546.1.CDS.4CDS
Spo08546.1.CDS.5Spo08546.1.CDS.5CDS


Homology
[Close]
BLAST of Spo08546.1 vs. NCBI nr
Match: gi|902196392|gb|KNA13112.1| (hypothetical protein SOVF_119660 [Spinacia oleracea])

HSP 1 Score: 321.2 bits (822), Expect = 1.300e-84
Identity = 165/165 (100.00%), Postives = 165/165 (100.00%), Query Frame = 1 

		  

Query: 49  RSCSLSLSSNGSSSSPSSTWSYSWHGSANARPPFLGHRPPMTLPIVRPPVVPVVMPAEKP 108
           RSCSLSLSSNGSSSSPSSTWSYSWHGSANARPPFLGHRPPMTLPIVRPPVVPVVMPAEKP
Sbjct: 12  RSCSLSLSSNGSSSSPSSTWSYSWHGSANARPPFLGHRPPMTLPIVRPPVVPVVMPAEKP 71

Query: 109 QTTVYVGKIASSVESDFVRSLLELCGPIKSWKRSQDLSDATPRRFGFFANGSETPASLSP 168
           QTTVYVGKIASSVESDFVRSLLELCGPIKSWKRSQDLSDATPRRFGFFANGSETPASLSP
Sbjct: 72  QTTVYVGKIASSVESDFVRSLLELCGPIKSWKRSQDLSDATPRRFGFFANGSETPASLSP 131

Query: 169 EFTASETKDDMEHDHDSAPALASAPVSVPSLAPDPASHTSLLYVS 214
           EFTASETKDDMEHDHDSAPALASAPVSVPSLAPDPASHTSLLYVS
Sbjct: 132 EFTASETKDDMEHDHDSAPALASAPVSVPSLAPDPASHTSLLYVS 176
[Close]
BLAST of Spo08546.1 vs. NCBI nr
Match: gi|902237817|gb|KNA24947.1| (hypothetical protein SOVF_011000 isoform A [Spinacia oleracea])

HSP 1 Score: 140.6 bits (353), Expect = 3.200e-30
Identity = 71/77 (92.21%), Postives = 72/77 (93.51%), Query Frame = 1 

		  

Query: 79  RPPFLGHRPPMTLPIVRPPVVPVVMPAEKPQTTVYVGKIASSVESDFVRSLLELCGPIKS 138
           RPPFLG RPPMTLPIVRPPV PVVMP EKPQTTVYVGKIASSVESDFVRSLLELCGPIKS
Sbjct: 225 RPPFLGLRPPMTLPIVRPPV-PVVMPVEKPQTTVYVGKIASSVESDFVRSLLELCGPIKS 284

Query: 139 WKRSQDLSDATPRRFGF 156
           WKR+QD SD TPRRFGF
Sbjct: 285 WKRAQDPSDGTPRRFGF 300
[Close]
BLAST of Spo08546.1 vs. NCBI nr
Match: gi|731342783|ref|XP_010682568.1| (PREDICTED: RNA-binding protein 25 isoform X2 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 131.3 bits (329), Expect = 1.900e-27
Identity = 63/77 (81.82%), Postives = 68/77 (88.31%), Query Frame = 1 

		  

Query: 79  RPPFLGHRPPMTLPIVRPPVVPVVMPAEKPQTTVYVGKIASSVESDFVRSLLELCGPIKS 138
           RPPFLG RPPMT PIVRPPVVP+V P EKPQTTVYVGKIA SVE+DF+ SLLELCG IKS
Sbjct: 39  RPPFLGLRPPMTPPIVRPPVVPIVAPVEKPQTTVYVGKIAPSVENDFIHSLLELCGTIKS 98

Query: 139 WKRSQDLSDATPRRFGF 156
           WKR+QD SD TP+RFGF
Sbjct: 99  WKRAQDPSDGTPKRFGF 115
[Close]
BLAST of Spo08546.1 vs. NCBI nr
Match: gi|731342785|ref|XP_010682570.1| (PREDICTED: RNA-binding protein 25 isoform X3 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 131.3 bits (329), Expect = 1.900e-27
Identity = 63/77 (81.82%), Postives = 68/77 (88.31%), Query Frame = 1 

		  

Query: 79  RPPFLGHRPPMTLPIVRPPVVPVVMPAEKPQTTVYVGKIASSVESDFVRSLLELCGPIKS 138
           RPPFLG RPPMT PIVRPPVVP+V P EKPQTTVYVGKIA SVE+DF+ SLLELCG IKS
Sbjct: 24  RPPFLGLRPPMTPPIVRPPVVPIVAPVEKPQTTVYVGKIAPSVENDFIHSLLELCGTIKS 83

Query: 139 WKRSQDLSDATPRRFGF 156
           WKR+QD SD TP+RFGF
Sbjct: 84  WKRAQDPSDGTPKRFGF 100
[Close]
BLAST of Spo08546.1 vs. NCBI nr
Match: gi|731342781|ref|XP_010682567.1| (PREDICTED: RNA-binding protein 25 isoform X1 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 131.3 bits (329), Expect = 1.900e-27
Identity = 63/77 (81.82%), Postives = 68/77 (88.31%), Query Frame = 1 

		  

Query: 79  RPPFLGHRPPMTLPIVRPPVVPVVMPAEKPQTTVYVGKIASSVESDFVRSLLELCGPIKS 138
           RPPFLG RPPMT PIVRPPVVP+V P EKPQTTVYVGKIA SVE+DF+ SLLELCG IKS
Sbjct: 190 RPPFLGLRPPMTPPIVRPPVVPIVAPVEKPQTTVYVGKIAPSVENDFIHSLLELCGTIKS 249

Query: 139 WKRSQDLSDATPRRFGF 156
           WKR+QD SD TP+RFGF
Sbjct: 250 WKRAQDPSDGTPKRFGF 266
[Close]
BLAST of Spo08546.1 vs. UniProtKB/TrEMBL
Match: A0A0K9R2V0_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_119660 PE=4 SV=1)

HSP 1 Score: 321.2 bits (822), Expect = 9.200e-85
Identity = 165/165 (100.00%), Postives = 165/165 (100.00%), Query Frame = 1 

		  

Query: 49  RSCSLSLSSNGSSSSPSSTWSYSWHGSANARPPFLGHRPPMTLPIVRPPVVPVVMPAEKP 108
           RSCSLSLSSNGSSSSPSSTWSYSWHGSANARPPFLGHRPPMTLPIVRPPVVPVVMPAEKP
Sbjct: 12  RSCSLSLSSNGSSSSPSSTWSYSWHGSANARPPFLGHRPPMTLPIVRPPVVPVVMPAEKP 71

Query: 109 QTTVYVGKIASSVESDFVRSLLELCGPIKSWKRSQDLSDATPRRFGFFANGSETPASLSP 168
           QTTVYVGKIASSVESDFVRSLLELCGPIKSWKRSQDLSDATPRRFGFFANGSETPASLSP
Sbjct: 72  QTTVYVGKIASSVESDFVRSLLELCGPIKSWKRSQDLSDATPRRFGFFANGSETPASLSP 131

Query: 169 EFTASETKDDMEHDHDSAPALASAPVSVPSLAPDPASHTSLLYVS 214
           EFTASETKDDMEHDHDSAPALASAPVSVPSLAPDPASHTSLLYVS
Sbjct: 132 EFTASETKDDMEHDHDSAPALASAPVSVPSLAPDPASHTSLLYVS 176
[Close]
BLAST of Spo08546.1 vs. UniProtKB/TrEMBL
Match: A0A0K9S1E2_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_011000 PE=4 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 2.200e-30
Identity = 71/77 (92.21%), Postives = 72/77 (93.51%), Query Frame = 1 

		  

Query: 79  RPPFLGHRPPMTLPIVRPPVVPVVMPAEKPQTTVYVGKIASSVESDFVRSLLELCGPIKS 138
           RPPFLG RPPMTLPIVRPPV PVVMP EKPQTTVYVGKIASSVESDFVRSLLELCGPIKS
Sbjct: 225 RPPFLGLRPPMTLPIVRPPV-PVVMPVEKPQTTVYVGKIASSVESDFVRSLLELCGPIKS 284

Query: 139 WKRSQDLSDATPRRFGF 156
           WKR+QD SD TPRRFGF
Sbjct: 285 WKRAQDPSDGTPRRFGF 300
[Close]
BLAST of Spo08546.1 vs. UniProtKB/TrEMBL
Match: A0A0J8C0H4_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_6g148970 PE=4 SV=1)

HSP 1 Score: 131.3 bits (329), Expect = 1.400e-27
Identity = 63/77 (81.82%), Postives = 68/77 (88.31%), Query Frame = 1 

		  

Query: 79  RPPFLGHRPPMTLPIVRPPVVPVVMPAEKPQTTVYVGKIASSVESDFVRSLLELCGPIKS 138
           RPPFLG RPPMT PIVRPPVVP+V P EKPQTTVYVGKIA SVE+DF+ SLLELCG IKS
Sbjct: 39  RPPFLGLRPPMTPPIVRPPVVPIVAPVEKPQTTVYVGKIAPSVENDFIHSLLELCGTIKS 98

Query: 139 WKRSQDLSDATPRRFGF 156
           WKR+QD SD TP+RFGF
Sbjct: 99  WKRAQDPSDGTPKRFGF 115
[Close]
BLAST of Spo08546.1 vs. UniProtKB/TrEMBL
Match: A0A0J8EVN0_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_6g148970 PE=4 SV=1)

HSP 1 Score: 131.3 bits (329), Expect = 1.400e-27
Identity = 63/77 (81.82%), Postives = 68/77 (88.31%), Query Frame = 1 

		  

Query: 79  RPPFLGHRPPMTLPIVRPPVVPVVMPAEKPQTTVYVGKIASSVESDFVRSLLELCGPIKS 138
           RPPFLG RPPMT PIVRPPVVP+V P EKPQTTVYVGKIA SVE+DF+ SLLELCG IKS
Sbjct: 190 RPPFLGLRPPMTPPIVRPPVVPIVAPVEKPQTTVYVGKIAPSVENDFIHSLLELCGTIKS 249

Query: 139 WKRSQDLSDATPRRFGF 156
           WKR+QD SD TP+RFGF
Sbjct: 250 WKRAQDPSDGTPKRFGF 266
[Close]
BLAST of Spo08546.1 vs. UniProtKB/TrEMBL
Match: E5GBJ9_CUCME (RNA binding motif protein OS=Cucumis melo subsp. melo PE=4 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 5.200e-19
Identity = 51/82 (62.20%), Postives = 63/82 (76.83%), Query Frame = 1 

		  

Query: 76  ANARPPFLGHRP--PMTLPIVRPPVVPVVMPAEKPQTTVYVGKIASSVESDFVRSLLELC 135
           A  RPP  G     P+  P+VRP V+P V PAEKPQTT+Y+G+IA SV++DF+ SLL+LC
Sbjct: 19  AMPRPPIPGIPGVRPIIPPVVRPAVLPSVTPAEKPQTTIYIGRIAQSVDNDFMLSLLQLC 78

Query: 136 GPIKSWKRSQDLSDATPRRFGF 156
           GP+KSWKR+QD SD TPR FGF
Sbjct: 79  GPVKSWKRAQDPSDGTPRTFGF 100
[Close]
BLAST of Spo08546.1 vs. TAIR (Arabidopsis)
Match: AT1G60200.1 (splicing factor PWI domain-containing protein / RNA recognition motif (RRM)-containing protein)

HSP 1 Score: 76.3 bits (186), Expect = 2.600e-14
Identity = 41/77 (53.25%), Postives = 55/77 (71.43%), Query Frame = 1 

		  

Query: 80  PPFLGHRPPMTLPIVRPPVVPVVMPAEKPQTTVYVGKIASSVESDFVRSLLELCGPIKSW 139
           P   G RP M  P+VRP  +P V PAEKPQTT+Y+GKIA +VE+DF+ S+LE CG +KS 
Sbjct: 175 PGMPGLRPVMP-PMVRPASLPFVTPAEKPQTTIYIGKIA-TVENDFMMSILEFCGHVKSC 234

Query: 140 KRSQDLSDATPRRFGFF 157
            R++D +   P+ FGF+
Sbjct: 235 LRAEDPTTKKPKGFGFY 249
The following BLAST results are available for this feature:
BLAST of Spo08546.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5
Match NameE-valueIdentityDescription
gi|902196392|gb|KNA13112.1|1.3e-84100.hypothetical protein SOVF_1196... [more]
gi|902237817|gb|KNA24947.1|3.2e-3092.2hypothetical protein SOVF_0110... [more]
gi|731342783|ref|XP_010682568.1|1.9e-2781.8PREDICTED: RNA-binding protein... [more]
gi|731342785|ref|XP_010682570.1|1.9e-2781.8PREDICTED: RNA-binding protein... [more]
gi|731342781|ref|XP_010682567.1|1.9e-2781.8PREDICTED: RNA-binding protein... [more]
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BLAST of Spo08546.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0K9R2V0_SPIOL9.2e-85100.Uncharacterized protein OS=Spi... [more]
A0A0K9S1E2_SPIOL2.2e-3092.2Uncharacterized protein OS=Spi... [more]
A0A0J8C0H4_BETVU1.4e-2781.8Uncharacterized protein OS=Bet... [more]
A0A0J8EVN0_BETVU1.4e-2781.8Uncharacterized protein OS=Bet... [more]
E5GBJ9_CUCME5.2e-1962.2RNA binding motif protein OS=C... [more]
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BLAST of Spo08546.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Spo08546.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 1
Match NameE-valueIdentityDescription
AT1G60200.12.6e-1453.2splicing factor PWI domain-con... [more]
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InterPro
Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012677Nucleotide-binding alpha-beta plait domainGENE3D3.30.70.330coord: 106..155
score: 5.
IPR012677Nucleotide-binding alpha-beta plait domainunknownSSF54928RNA-binding domain, RBDcoord: 69..155
score: 5.9
NoneNo IPR availablePANTHERPTHR18806RBM25 PROTEINcoord: 80..155
score: 3.4

GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006397 mRNA processing
molecular_function GO:0000166 nucleotide binding
molecular_function GO:0003676 nucleic acid binding