Spo08745.1 (mRNA) | SpinachBase

Overview


Name	Spo08745.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	BRI1 kinase inhibitor 1
Location	chr3 : 113373637 .. 113374641 (-)
Sequence length	1005

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATAATACTGATACACATCAACACGAAAATCGCATAGTAACAGGTGCAGCAGTGAATTCGCCACCGGTGCTTTCAGCGTCGTCTTCACCATCACATGAATTCTCATTTACAATCTCATTGGATTCCTCAGTGAAAACCACCACGAAAGCATCGTTGTCGCCTTCTGTAACAGAAAAAACTAAGCAGCAGCAGCAAGGTCCATTAGCTATTGATTTATCTCCTGCAGATGAAATCTTTTTCCATGGTCATTTACTTCCTCTTCACTTCCTATCCCACCTCCACGCTTCCTCTGCTCGCCCCTCGATTCATTCATTGGATAGCTTTTCGGGTCCGATTCAAGATGTGTTACAGGAAGAAAAGCCGCTAACAAGTAGTACTATAGAAAACAGCAGCAGCAGTTGTAGCTGTACTGAAGACGACCACTGCATCTCATTCGACAAAGATGACAACGAAATTAAATGTAATATCGATTTCAGTAGTCACCACAAATGCATCAAAAAGGAAAGAAGCAGCAATAATAAATCAAAATCGTTTTCTCTCTTTGGATTATCCAAATGGAAAAAACCGTCAGAAGGTACAGAGAAGTCTCAAGTCATAGAAAACTGCAGCAAGCGAAACAAGGTTAAGTTTGATGTGACTCAATTCCTTAAAAGGTACATAAATATGGTGAAGCCACTTTTATCTCCAAAAAGTGGTGGGCGAAGAGATAATGTACGAGGTCGAAGGCAATCGTACTCATTCTCAGGGACGGTCACTCCTCGAGATAATCGAGAGATAATAAGAGGAAGACGAGGAGAGTTTTCAGCTCCTGCATCCATGAGAACTTCACCTACAAACAGCGGTCTTCTTTTGGCTACTGCAGGAGTCCCATCACCTGTAAGTGAGAGTACCATGGAAGACTTGCAGGCTGCTATTCAAGCTGCTATTGCTCATTGCAAAAATTCTATTTCAACTCAAGAGAATAAATCACATGAAATATCATCATCAATTCCTGTTAATTAG

mRNA sequence

ATGGATAATACTGATACACATCAACACGAAAATCGCATAGTAACAGGTGCAGCAGTGAATTCGCCACCGGTGCTTTCAGCGTCGTCTTCACCATCACATGAATTCTCATTTACAATCTCATTGGATTCCTCAGTGAAAACCACCACGAAAGCATCGTTGTCGCCTTCTGTAACAGAAAAAACTAAGCAGCAGCAGCAAGGTCCATTAGCTATTGATTTATCTCCTGCAGATGAAATCTTTTTCCATGGTCATTTACTTCCTCTTCACTTCCTATCCCACCTCCACGCTTCCTCTGCTCGCCCCTCGATTCATTCATTGGATAGCTTTTCGGGTCCGATTCAAGATGTGTTACAGGAAGAAAAGCCGCTAACAAGTAGTACTATAGAAAACAGCAGCAGCAGTTGTAGCTGTACTGAAGACGACCACTGCATCTCATTCGACAAAGATGACAACGAAATTAAATGTAATATCGATTTCAGTAGTCACCACAAATGCATCAAAAAGGAAAGAAGCAGCAATAATAAATCAAAATCGTTTTCTCTCTTTGGATTATCCAAATGGAAAAAACCGTCAGAAGGTACAGAGAAGTCTCAAGTCATAGAAAACTGCAGCAAGCGAAACAAGGTTAAGTTTGATGTGACTCAATTCCTTAAAAGGTACATAAATATGGTGAAGCCACTTTTATCTCCAAAAAGTGGTGGGCGAAGAGATAATGTACGAGGTCGAAGGCAATCGTACTCATTCTCAGGGACGGTCACTCCTCGAGATAATCGAGAGATAATAAGAGGAAGACGAGGAGAGTTTTCAGCTCCTGCATCCATGAGAACTTCACCTACAAACAGCGGTCTTCTTTTGGCTACTGCAGGAGTCCCATCACCTGTAAGTGAGAGTACCATGGAAGACTTGCAGGCTGCTATTCAAGCTGCTATTGCTCATTGCAAAAATTCTATTTCAACTCAAGAGAATAAATCACATGAAATATCATCATCAATTCCTGTTAATTAG

Coding sequence (CDS)

ATGGATAATACTGATACACATCAACACGAAAATCGCATAGTAACAGGTGCAGCAGTGAATTCGCCACCGGTGCTTTCAGCGTCGTCTTCACCATCACATGAATTCTCATTTACAATCTCATTGGATTCCTCAGTGAAAACCACCACGAAAGCATCGTTGTCGCCTTCTGTAACAGAAAAAACTAAGCAGCAGCAGCAAGGTCCATTAGCTATTGATTTATCTCCTGCAGATGAAATCTTTTTCCATGGTCATTTACTTCCTCTTCACTTCCTATCCCACCTCCACGCTTCCTCTGCTCGCCCCTCGATTCATTCATTGGATAGCTTTTCGGGTCCGATTCAAGATGTGTTACAGGAAGAAAAGCCGCTAACAAGTAGTACTATAGAAAACAGCAGCAGCAGTTGTAGCTGTACTGAAGACGACCACTGCATCTCATTCGACAAAGATGACAACGAAATTAAATGTAATATCGATTTCAGTAGTCACCACAAATGCATCAAAAAGGAAAGAAGCAGCAATAATAAATCAAAATCGTTTTCTCTCTTTGGATTATCCAAATGGAAAAAACCGTCAGAAGGTACAGAGAAGTCTCAAGTCATAGAAAACTGCAGCAAGCGAAACAAGGTTAAGTTTGATGTGACTCAATTCCTTAAAAGGTACATAAATATGGTGAAGCCACTTTTATCTCCAAAAAGTGGTGGGCGAAGAGATAATGTACGAGGTCGAAGGCAATCGTACTCATTCTCAGGGACGGTCACTCCTCGAGATAATCGAGAGATAATAAGAGGAAGACGAGGAGAGTTTTCAGCTCCTGCATCCATGAGAACTTCACCTACAAACAGCGGTCTTCTTTTGGCTACTGCAGGAGTCCCATCACCTGTAAGTGAGAGTACCATGGAAGACTTGCAGGCTGCTATTCAAGCTGCTATTGCTCATTGCAAAAATTCTATTTCAACTCAAGAGAATAAATCACATGAAATATCATCATCAATTCCTGTTAATTAG

Protein sequence

MDNTDTHQHENRIVTGAAVNSPPVLSASSSPSHEFSFTISLDSSVKTTTKASLSPSVTEKTKQQQQGPLAIDLSPADEIFFHGHLLPLHFLSHLHASSARPSIHSLDSFSGPIQDVLQEEKPLTSSTIENSSSSCSCTEDDHCISFDKDDNEIKCNIDFSSHHKCIKKERSSNNKSKSFSLFGLSKWKKPSEGTEKSQVIENCSKRNKVKFDVTQFLKRYINMVKPLLSPKSGGRRDNVRGRRQSYSFSGTVTPRDNREIIRGRRGEFSAPASMRTSPTNSGLLLATAGVPSPVSESTMEDLQAAIQAAIAHCKNSISTQENKSHEISSSIPVN

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo08745	Spo08745	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo08745.1	Spo08745.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo08745.1.CDS.1	Spo08745.1.CDS.1	CDS

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo08745.1.exon.1	Spo08745.1.exon.1	exon

Homology

[Close]

BLAST of Spo08745.1 vs. NCBI nr
Match: gi|902220225|gb|KNA18535.1| (hypothetical protein SOVF_069890 [Spinacia oleracea])

HSP 1 Score: 641.0 bits (1652), Expect = 1.200e-180
Identity = 334/334 (100.00%), Postives = 334/334 (100.00%), Query Frame = 1


		  
Query: 1   MDNTDTHQHENRIVTGAAVNSPPVLSASSSPSHEFSFTISLDSSVKTTTKASLSPSVTEK 60
           MDNTDTHQHENRIVTGAAVNSPPVLSASSSPSHEFSFTISLDSSVKTTTKASLSPSVTEK
Sbjct: 1   MDNTDTHQHENRIVTGAAVNSPPVLSASSSPSHEFSFTISLDSSVKTTTKASLSPSVTEK 60

Query: 61  TKQQQQGPLAIDLSPADEIFFHGHLLPLHFLSHLHASSARPSIHSLDSFSGPIQDVLQEE 120
           TKQQQQGPLAIDLSPADEIFFHGHLLPLHFLSHLHASSARPSIHSLDSFSGPIQDVLQEE
Sbjct: 61  TKQQQQGPLAIDLSPADEIFFHGHLLPLHFLSHLHASSARPSIHSLDSFSGPIQDVLQEE 120

Query: 121 KPLTSSTIENSSSSCSCTEDDHCISFDKDDNEIKCNIDFSSHHKCIKKERSSNNKSKSFS 180
           KPLTSSTIENSSSSCSCTEDDHCISFDKDDNEIKCNIDFSSHHKCIKKERSSNNKSKSFS
Sbjct: 121 KPLTSSTIENSSSSCSCTEDDHCISFDKDDNEIKCNIDFSSHHKCIKKERSSNNKSKSFS 180

Query: 181 LFGLSKWKKPSEGTEKSQVIENCSKRNKVKFDVTQFLKRYINMVKPLLSPKSGGRRDNVR 240
           LFGLSKWKKPSEGTEKSQVIENCSKRNKVKFDVTQFLKRYINMVKPLLSPKSGGRRDNVR
Sbjct: 181 LFGLSKWKKPSEGTEKSQVIENCSKRNKVKFDVTQFLKRYINMVKPLLSPKSGGRRDNVR 240

Query: 241 GRRQSYSFSGTVTPRDNREIIRGRRGEFSAPASMRTSPTNSGLLLATAGVPSPVSESTME 300
           GRRQSYSFSGTVTPRDNREIIRGRRGEFSAPASMRTSPTNSGLLLATAGVPSPVSESTME
Sbjct: 241 GRRQSYSFSGTVTPRDNREIIRGRRGEFSAPASMRTSPTNSGLLLATAGVPSPVSESTME 300

Query: 301 DLQAAIQAAIAHCKNSISTQENKSHEISSSIPVN 335
           DLQAAIQAAIAHCKNSISTQENKSHEISSSIPVN
Sbjct: 301 DLQAAIQAAIAHCKNSISTQENKSHEISSSIPVN 334

[Close]

BLAST of Spo08745.1 vs. NCBI nr
Match: gi|731362252|ref|XP_010692794.1| (PREDICTED: BRI1 kinase inhibitor 1 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 354.8 bits (909), Expect = 1.700e-94
Identity = 223/352 (63.35%), Postives = 264/352 (75.00%), Query Frame = 1


		  
Query: 2   DNTDTHQHENRIVTGAAVNSPPVLSASSSPSHEFSFTISLDSSVKTTTKASLSPSVTEKT 61
           + ++    + R       NS PV+SASSSPSHEFSFTISL SS  TTT A+ +P+  +K+
Sbjct: 16  NRSNLEPQQQRATPATIANSSPVISASSSPSHEFSFTISLHSS--TTTTATSNPASIDKS 75

Query: 62  KQQQQGP-------------LAIDLSPADEIFFHGHLLPLHFLSHLHASSARPSIHSLDS 121
           KQQQQ               LAIDLSPADEIFFHGHLLPLHFLSHLH SS RPS HSLDS
Sbjct: 76  KQQQQQQQQQQQQQQSQPPSLAIDLSPADEIFFHGHLLPLHFLSHLHTSSPRPSTHSLDS 135

Query: 122 FSGPIQDVLQEEKPLTSSTIENSSSSCS-CTEDDHCISFDKDDNEIKCNIDFSSHHKCIK 181
           F+ P +   ++       T ENS+ SC+ C EDDHCISF+K+ N+ + NI+ + H  C  
Sbjct: 136 FALPNKHESKDR------TTENSNISCNTCNEDDHCISFNKE-NDDESNIEINLH-SCQT 195

Query: 182 KERSSNNKSKS-FSLFGLSKWKK-PSEGTEKSQVIENCS--KRNKVKFDVTQFLKRYINM 241
            +  +N+KSKS FSLFGLSK KK P+E  EKS   E  S  K  K+KFDV+QFLKRY+NM
Sbjct: 196 AKEGTNDKSKSSFSLFGLSKRKKAPTEVKEKSSS-EGISEHKSRKLKFDVSQFLKRYLNM 255

Query: 242 VKPLLSPKSGGRRDNVRGRRQSYSFSGTVTPRDNRE-IIRGRRGEFSAPASMRTSPTNSG 301
           VKPLLSP++G R +NVRGRRQSYSFSGTVTPRDNRE ++RG+RGEFSAPASMRTSPTNSG
Sbjct: 256 VKPLLSPRAGRRDNNVRGRRQSYSFSGTVTPRDNREGLMRGKRGEFSAPASMRTSPTNSG 315

Query: 302 LLLATAGVPSPVSESTMEDLQAAIQAAIAHCKNSISTQENKSHEISSSIPVN 335
           LLLATAGV SPVS+STME+LQAAIQAAIAHCKNSIS+ E+KS E+ SS+PVN
Sbjct: 316 LLLATAGVASPVSDSTMEELQAAIQAAIAHCKNSISS-EDKSQEL-SSVPVN 354

[Close]

BLAST of Spo08745.1 vs. NCBI nr
Match: gi|225458591|ref|XP_002284646.1| (PREDICTED: BRI1 kinase inhibitor 1 [Vitis vinifera])

HSP 1 Score: 248.1 bits (632), Expect = 2.200e-62
Identity = 166/304 (54.61%), Postives = 207/304 (68.09%), Query Frame = 1


		  
Query: 21  SPPVLSASSSPSHEFSFTISLDSSVKTTTKASLSPSVTEKTKQQQQGPLAIDLSPADEIF 80
           SPP  SASSSPSHEFSFTISL  S         S ++ +K K Q     AIDLSPAD+IF
Sbjct: 36  SPP--SASSSPSHEFSFTISLHPS---------STTIPDKAKAQTS-TFAIDLSPADDIF 95

Query: 81  FHGHLLPLHFLSHLHASSARPSIHSLDSFSGPIQDVLQEEKPLTSSTIENSSSSCSCTED 140
           FHGHLLPLH LSHL   S R S +SLDSF+ PI+++L+++KP      EN+ S+CS + +
Sbjct: 96  FHGHLLPLHLLSHLPV-SPRSSTNSLDSFTLPIRELLEDQKP------ENNGSNCSSSSN 155

Query: 141 DHCISFDKDDNEIKCNIDFSS---HHKCIKKERSSNNKSKSFSLFGLSKWKKPSEGTEKS 200
              I   K     + NI  SS   H +    E    NKSKSFSLFG  +W+K  E  +K 
Sbjct: 156 SQNI---KRSCNSENNISSSSNRRHQQSNSSETKERNKSKSFSLFG--RWRKGCEIGDKE 215

Query: 201 QVIENCSKRNKVKFDVTQFLKRYINMVKPLLSPKSGGRRDNVRGRRQSYSFSGTVTPRDN 260
              E   ++ K++FDV+  LKRY+ MV+PLL  +  GRRDN++ RRQ YSFSG ++ R  
Sbjct: 216 ---EKEKQKRKLRFDVSHVLKRYVRMVRPLLFFR--GRRDNIQFRRQPYSFSGNLSLRGK 275

Query: 261 REIIRGRRGEFSAPASMRTSPTNSGLLLATAGVPSPVSESTMEDLQAAIQAAIAHCKNSI 320
           +E +RGRRGEFSAPASMRTSPTNSGLL+ATA +PS  S+STME+LQAAIQAAIAHCKNSI
Sbjct: 276 QE-LRGRRGEFSAPASMRTSPTNSGLLVATATLPSSTSDSTMEELQAAIQAAIAHCKNSI 309

Query: 321 STQE 322
           + +E
Sbjct: 336 AVEE 309

[Close]

BLAST of Spo08745.1 vs. NCBI nr
Match: gi|590592448|ref|XP_007017284.1| (BRI1 kinase inhibitor 1 [Theobroma cacao])

HSP 1 Score: 247.3 bits (630), Expect = 3.800e-62
Identity = 164/314 (52.23%), Postives = 210/314 (66.88%), Query Frame = 1


		  
Query: 17  AAVNSPPVLSASSSPSHEFSFTISLDSSVKTTTKASLSPSVTEKTKQQQQGPLAIDLSPA 76
           A+  SPP  SASSSPSHEFSFTISL SS  T         V +KTK      +AIDLSPA
Sbjct: 40  ASPASPP--SASSSPSHEFSFTISLHSSSNT---------VPDKTKTPPS--IAIDLSPA 99

Query: 77  DEIFFHGHLLPLHFLSHLHASSARPSIHSLDSFSGPIQDVLQEEKPLTSSTIENSSSSCS 136
           D+IFFHGHLLPLH LSHL  S  R S +SLDSF+ P++++L ++KP      + SSS+ S
Sbjct: 100 DDIFFHGHLLPLHLLSHLPVSP-RSSTNSLDSFTLPVRELLDDQKP------DKSSSNRS 159

Query: 137 CTEDDHCISFDKDDNEIKCNIDFSSHHKCIKKERSSNN-------KSKSFSLFGLSKWKK 196
                      K D+ I+ NI+ S +H  +     SN+       KSKSFS+F L++W+K
Sbjct: 160 -----------KSDSNIRTNINRSKNHDKVSNRHQSNDIEGEGRSKSKSFSIFSLTRWQK 219

Query: 197 PSEGTEKSQVIENCSKRNKVKFDVTQFLKRYINMVKPLLSPKSGGRRDNVRGRRQSYSFS 256
             +  E  +  ++  K  K++FDV+  LKRY+ MV+PLL  +  GRRDN+  RRQ+YSFS
Sbjct: 220 GRDVRETQEKEKHNKK--KMRFDVSHVLKRYVRMVRPLLFFR--GRRDNLHLRRQAYSFS 279

Query: 257 GTVTPRDNREIIRGRRGEFSAPASMRTSPTNSGLLLATAGVPSPVSESTMEDLQAAIQAA 316
           G ++ R N++ +RGRRGE+SAPASMRTSPTNSGLL+AT G PS  S+STME+LQAAIQAA
Sbjct: 280 GNLSLR-NKQELRGRRGEYSAPASMRTSPTNSGLLVATTGFPSSTSDSTMEELQAAIQAA 317

Query: 317 IAHCKNSISTQENK 324
           IAHCKNSI   + K
Sbjct: 340 IAHCKNSIGEDKLK 317

[Close]

BLAST of Spo08745.1 vs. NCBI nr
Match: gi|147866713|emb|CAN78408.1| (hypothetical protein VITISV_023177 [Vitis vinifera])

HSP 1 Score: 246.9 bits (629), Expect = 5.000e-62
Identity = 165/304 (54.28%), Postives = 206/304 (67.76%), Query Frame = 1


		  
Query: 21  SPPVLSASSSPSHEFSFTISLDSSVKTTTKASLSPSVTEKTKQQQQGPLAIDLSPADEIF 80
           SPP  SASSSPSHEFSFTISL  S         S ++ +K K Q     AIDLSPAD+IF
Sbjct: 98  SPP--SASSSPSHEFSFTISLHPS---------STTIPDKAKAQTS-TFAIDLSPADDIF 157

Query: 81  FHGHLLPLHFLSHLHASSARPSIHSLDSFSGPIQDVLQEEKPLTSSTIENSSSSCSCTED 140
           FHGHLLPLH LSHL   S R S +SLDSF+ PI+++L+++KP      EN+ S+CS + +
Sbjct: 158 FHGHLLPLHLLSHLPV-SPRSSXNSLDSFTLPIRELLEDQKP------ENNGSNCSSSSN 217

Query: 141 DHCISFDKDDNEIKCNIDFSS---HHKCIKKERSSNNKSKSFSLFGLSKWKKPSEGTEKS 200
              I   K     + NI  SS   H +    E    NKSK FSLFG  +W+K  E  +K 
Sbjct: 218 SQNI---KRSCNSENNISSSSNRRHQQSNSSETKERNKSKXFSLFG--RWRKGCEIGDKE 277

Query: 201 QVIENCSKRNKVKFDVTQFLKRYINMVKPLLSPKSGGRRDNVRGRRQSYSFSGTVTPRDN 260
              E   ++ K++FDV+  LKRY+ MV+PLL  +  GRRDN++ RRQ YSFSG ++ R  
Sbjct: 278 ---EKEKQKRKLRFDVSHVLKRYVRMVRPLLFFR--GRRDNIQXRRQPYSFSGNLSLRGK 337

Query: 261 REIIRGRRGEFSAPASMRTSPTNSGLLLATAGVPSPVSESTMEDLQAAIQAAIAHCKNSI 320
           +E +RGRRGEFSAPASMRTSPTNSGLL+ATA +PS  S+STME+LQAAIQAAIAHCKNSI
Sbjct: 338 QE-LRGRRGEFSAPASMRTSPTNSGLLVATATLPSSTSDSTMEELQAAIQAAIAHCKNSI 371

Query: 321 STQE 322
           + +E
Sbjct: 398 AVEE 371

[Close]

BLAST of Spo08745.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RGD0_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_069890 PE=4 SV=1)

HSP 1 Score: 641.0 bits (1652), Expect = 8.300e-181
Identity = 334/334 (100.00%), Postives = 334/334 (100.00%), Query Frame = 1


		  
Query: 1   MDNTDTHQHENRIVTGAAVNSPPVLSASSSPSHEFSFTISLDSSVKTTTKASLSPSVTEK 60
           MDNTDTHQHENRIVTGAAVNSPPVLSASSSPSHEFSFTISLDSSVKTTTKASLSPSVTEK
Sbjct: 1   MDNTDTHQHENRIVTGAAVNSPPVLSASSSPSHEFSFTISLDSSVKTTTKASLSPSVTEK 60

Query: 61  TKQQQQGPLAIDLSPADEIFFHGHLLPLHFLSHLHASSARPSIHSLDSFSGPIQDVLQEE 120
           TKQQQQGPLAIDLSPADEIFFHGHLLPLHFLSHLHASSARPSIHSLDSFSGPIQDVLQEE
Sbjct: 61  TKQQQQGPLAIDLSPADEIFFHGHLLPLHFLSHLHASSARPSIHSLDSFSGPIQDVLQEE 120

Query: 121 KPLTSSTIENSSSSCSCTEDDHCISFDKDDNEIKCNIDFSSHHKCIKKERSSNNKSKSFS 180
           KPLTSSTIENSSSSCSCTEDDHCISFDKDDNEIKCNIDFSSHHKCIKKERSSNNKSKSFS
Sbjct: 121 KPLTSSTIENSSSSCSCTEDDHCISFDKDDNEIKCNIDFSSHHKCIKKERSSNNKSKSFS 180

Query: 181 LFGLSKWKKPSEGTEKSQVIENCSKRNKVKFDVTQFLKRYINMVKPLLSPKSGGRRDNVR 240
           LFGLSKWKKPSEGTEKSQVIENCSKRNKVKFDVTQFLKRYINMVKPLLSPKSGGRRDNVR
Sbjct: 181 LFGLSKWKKPSEGTEKSQVIENCSKRNKVKFDVTQFLKRYINMVKPLLSPKSGGRRDNVR 240

Query: 241 GRRQSYSFSGTVTPRDNREIIRGRRGEFSAPASMRTSPTNSGLLLATAGVPSPVSESTME 300
           GRRQSYSFSGTVTPRDNREIIRGRRGEFSAPASMRTSPTNSGLLLATAGVPSPVSESTME
Sbjct: 241 GRRQSYSFSGTVTPRDNREIIRGRRGEFSAPASMRTSPTNSGLLLATAGVPSPVSESTME 300

Query: 301 DLQAAIQAAIAHCKNSISTQENKSHEISSSIPVN 335
           DLQAAIQAAIAHCKNSISTQENKSHEISSSIPVN
Sbjct: 301 DLQAAIQAAIAHCKNSISTQENKSHEISSSIPVN 334

[Close]

BLAST of Spo08745.1 vs. UniProtKB/TrEMBL
Match: A0A0J8E8Z9_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_1g022700 PE=4 SV=1)

HSP 1 Score: 354.8 bits (909), Expect = 1.200e-94
Identity = 223/352 (63.35%), Postives = 264/352 (75.00%), Query Frame = 1


		  
Query: 2   DNTDTHQHENRIVTGAAVNSPPVLSASSSPSHEFSFTISLDSSVKTTTKASLSPSVTEKT 61
           + ++    + R       NS PV+SASSSPSHEFSFTISL SS  TTT A+ +P+  +K+
Sbjct: 16  NRSNLEPQQQRATPATIANSSPVISASSSPSHEFSFTISLHSS--TTTTATSNPASIDKS 75

Query: 62  KQQQQGP-------------LAIDLSPADEIFFHGHLLPLHFLSHLHASSARPSIHSLDS 121
           KQQQQ               LAIDLSPADEIFFHGHLLPLHFLSHLH SS RPS HSLDS
Sbjct: 76  KQQQQQQQQQQQQQQSQPPSLAIDLSPADEIFFHGHLLPLHFLSHLHTSSPRPSTHSLDS 135

Query: 122 FSGPIQDVLQEEKPLTSSTIENSSSSCS-CTEDDHCISFDKDDNEIKCNIDFSSHHKCIK 181
           F+ P +   ++       T ENS+ SC+ C EDDHCISF+K+ N+ + NI+ + H  C  
Sbjct: 136 FALPNKHESKDR------TTENSNISCNTCNEDDHCISFNKE-NDDESNIEINLH-SCQT 195

Query: 182 KERSSNNKSKS-FSLFGLSKWKK-PSEGTEKSQVIENCS--KRNKVKFDVTQFLKRYINM 241
            +  +N+KSKS FSLFGLSK KK P+E  EKS   E  S  K  K+KFDV+QFLKRY+NM
Sbjct: 196 AKEGTNDKSKSSFSLFGLSKRKKAPTEVKEKSSS-EGISEHKSRKLKFDVSQFLKRYLNM 255

Query: 242 VKPLLSPKSGGRRDNVRGRRQSYSFSGTVTPRDNRE-IIRGRRGEFSAPASMRTSPTNSG 301
           VKPLLSP++G R +NVRGRRQSYSFSGTVTPRDNRE ++RG+RGEFSAPASMRTSPTNSG
Sbjct: 256 VKPLLSPRAGRRDNNVRGRRQSYSFSGTVTPRDNREGLMRGKRGEFSAPASMRTSPTNSG 315

Query: 302 LLLATAGVPSPVSESTMEDLQAAIQAAIAHCKNSISTQENKSHEISSSIPVN 335
           LLLATAGV SPVS+STME+LQAAIQAAIAHCKNSIS+ E+KS E+ SS+PVN
Sbjct: 316 LLLATAGVASPVSDSTMEELQAAIQAAIAHCKNSISS-EDKSQEL-SSVPVN 354

[Close]

BLAST of Spo08745.1 vs. UniProtKB/TrEMBL
Match: F6H005_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_18s0001g10690 PE=4 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 1.600e-62
Identity = 166/304 (54.61%), Postives = 207/304 (68.09%), Query Frame = 1


		  
Query: 21  SPPVLSASSSPSHEFSFTISLDSSVKTTTKASLSPSVTEKTKQQQQGPLAIDLSPADEIF 80
           SPP  SASSSPSHEFSFTISL  S         S ++ +K K Q     AIDLSPAD+IF
Sbjct: 36  SPP--SASSSPSHEFSFTISLHPS---------STTIPDKAKAQTS-TFAIDLSPADDIF 95

Query: 81  FHGHLLPLHFLSHLHASSARPSIHSLDSFSGPIQDVLQEEKPLTSSTIENSSSSCSCTED 140
           FHGHLLPLH LSHL   S R S +SLDSF+ PI+++L+++KP      EN+ S+CS + +
Sbjct: 96  FHGHLLPLHLLSHLPV-SPRSSTNSLDSFTLPIRELLEDQKP------ENNGSNCSSSSN 155

Query: 141 DHCISFDKDDNEIKCNIDFSS---HHKCIKKERSSNNKSKSFSLFGLSKWKKPSEGTEKS 200
              I   K     + NI  SS   H +    E    NKSKSFSLFG  +W+K  E  +K 
Sbjct: 156 SQNI---KRSCNSENNISSSSNRRHQQSNSSETKERNKSKSFSLFG--RWRKGCEIGDKE 215

Query: 201 QVIENCSKRNKVKFDVTQFLKRYINMVKPLLSPKSGGRRDNVRGRRQSYSFSGTVTPRDN 260
              E   ++ K++FDV+  LKRY+ MV+PLL  +  GRRDN++ RRQ YSFSG ++ R  
Sbjct: 216 ---EKEKQKRKLRFDVSHVLKRYVRMVRPLLFFR--GRRDNIQFRRQPYSFSGNLSLRGK 275

Query: 261 REIIRGRRGEFSAPASMRTSPTNSGLLLATAGVPSPVSESTMEDLQAAIQAAIAHCKNSI 320
           +E +RGRRGEFSAPASMRTSPTNSGLL+ATA +PS  S+STME+LQAAIQAAIAHCKNSI
Sbjct: 276 QE-LRGRRGEFSAPASMRTSPTNSGLLVATATLPSSTSDSTMEELQAAIQAAIAHCKNSI 309

Query: 321 STQE 322
           + +E
Sbjct: 336 AVEE 309

[Close]

BLAST of Spo08745.1 vs. UniProtKB/TrEMBL
Match: A0A061FJ36_THECC (BRI1 kinase inhibitor 1 OS=Theobroma cacao GN=TCM_033868 PE=4 SV=1)

HSP 1 Score: 247.3 bits (630), Expect = 2.700e-62
Identity = 164/314 (52.23%), Postives = 210/314 (66.88%), Query Frame = 1


		  
Query: 17  AAVNSPPVLSASSSPSHEFSFTISLDSSVKTTTKASLSPSVTEKTKQQQQGPLAIDLSPA 76
           A+  SPP  SASSSPSHEFSFTISL SS  T         V +KTK      +AIDLSPA
Sbjct: 40  ASPASPP--SASSSPSHEFSFTISLHSSSNT---------VPDKTKTPPS--IAIDLSPA 99

Query: 77  DEIFFHGHLLPLHFLSHLHASSARPSIHSLDSFSGPIQDVLQEEKPLTSSTIENSSSSCS 136
           D+IFFHGHLLPLH LSHL  S  R S +SLDSF+ P++++L ++KP      + SSS+ S
Sbjct: 100 DDIFFHGHLLPLHLLSHLPVSP-RSSTNSLDSFTLPVRELLDDQKP------DKSSSNRS 159

Query: 137 CTEDDHCISFDKDDNEIKCNIDFSSHHKCIKKERSSNN-------KSKSFSLFGLSKWKK 196
                      K D+ I+ NI+ S +H  +     SN+       KSKSFS+F L++W+K
Sbjct: 160 -----------KSDSNIRTNINRSKNHDKVSNRHQSNDIEGEGRSKSKSFSIFSLTRWQK 219

Query: 197 PSEGTEKSQVIENCSKRNKVKFDVTQFLKRYINMVKPLLSPKSGGRRDNVRGRRQSYSFS 256
             +  E  +  ++  K  K++FDV+  LKRY+ MV+PLL  +  GRRDN+  RRQ+YSFS
Sbjct: 220 GRDVRETQEKEKHNKK--KMRFDVSHVLKRYVRMVRPLLFFR--GRRDNLHLRRQAYSFS 279

Query: 257 GTVTPRDNREIIRGRRGEFSAPASMRTSPTNSGLLLATAGVPSPVSESTMEDLQAAIQAA 316
           G ++ R N++ +RGRRGE+SAPASMRTSPTNSGLL+AT G PS  S+STME+LQAAIQAA
Sbjct: 280 GNLSLR-NKQELRGRRGEYSAPASMRTSPTNSGLLVATTGFPSSTSDSTMEELQAAIQAA 317

Query: 317 IAHCKNSISTQENK 324
           IAHCKNSI   + K
Sbjct: 340 IAHCKNSIGEDKLK 317

[Close]

BLAST of Spo08745.1 vs. UniProtKB/TrEMBL
Match: A5AWQ1_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_023177 PE=4 SV=1)

HSP 1 Score: 246.9 bits (629), Expect = 3.500e-62
Identity = 165/304 (54.28%), Postives = 206/304 (67.76%), Query Frame = 1


		  
Query: 21  SPPVLSASSSPSHEFSFTISLDSSVKTTTKASLSPSVTEKTKQQQQGPLAIDLSPADEIF 80
           SPP  SASSSPSHEFSFTISL  S         S ++ +K K Q     AIDLSPAD+IF
Sbjct: 98  SPP--SASSSPSHEFSFTISLHPS---------STTIPDKAKAQTS-TFAIDLSPADDIF 157

Query: 81  FHGHLLPLHFLSHLHASSARPSIHSLDSFSGPIQDVLQEEKPLTSSTIENSSSSCSCTED 140
           FHGHLLPLH LSHL   S R S +SLDSF+ PI+++L+++KP      EN+ S+CS + +
Sbjct: 158 FHGHLLPLHLLSHLPV-SPRSSXNSLDSFTLPIRELLEDQKP------ENNGSNCSSSSN 217

Query: 141 DHCISFDKDDNEIKCNIDFSS---HHKCIKKERSSNNKSKSFSLFGLSKWKKPSEGTEKS 200
              I   K     + NI  SS   H +    E    NKSK FSLFG  +W+K  E  +K 
Sbjct: 218 SQNI---KRSCNSENNISSSSNRRHQQSNSSETKERNKSKXFSLFG--RWRKGCEIGDKE 277

Query: 201 QVIENCSKRNKVKFDVTQFLKRYINMVKPLLSPKSGGRRDNVRGRRQSYSFSGTVTPRDN 260
              E   ++ K++FDV+  LKRY+ MV+PLL  +  GRRDN++ RRQ YSFSG ++ R  
Sbjct: 278 ---EKEKQKRKLRFDVSHVLKRYVRMVRPLLFFR--GRRDNIQXRRQPYSFSGNLSLRGK 337

Query: 261 REIIRGRRGEFSAPASMRTSPTNSGLLLATAGVPSPVSESTMEDLQAAIQAAIAHCKNSI 320
           +E +RGRRGEFSAPASMRTSPTNSGLL+ATA +PS  S+STME+LQAAIQAAIAHCKNSI
Sbjct: 338 QE-LRGRRGEFSAPASMRTSPTNSGLLVATATLPSSTSDSTMEELQAAIQAAIAHCKNSI 371

Query: 321 STQE 322
           + +E
Sbjct: 398 AVEE 371

[Close]

BLAST of Spo08745.1 vs. ExPASy Swiss-Prot
Match: BKI1_ARATH (BRI1 kinase inhibitor 1 OS=Arabidopsis thaliana GN=BKI1 PE=1 SV=1)

HSP 1 Score: 185.3 bits (469), Expect = 1.100e-45
Identity = 140/318 (44.03%), Postives = 180/318 (56.60%), Query Frame = 1


		  
Query: 23  PVLSASSSPSHEFSFTISLD---SSVKTTTKASLSPSVTEKTKQQQQGPLAIDLSPADEI 82
           P+ S  SSPSH+FSFTISL    SS K  +    SPS T  + QQ   P A+DLSPADEI
Sbjct: 29  PISSTCSSPSHDFSFTISLQPLSSSSKHISPTLRSPSKTTSSYQQTD-PFAVDLSPADEI 88

Query: 83  FFHGHLLPLHFLSHLHASSARPSIHSLDSFSGPIQDVLQEEKPLTSSTIENSSSSCSCTE 142
           FFHGHLLPLH LSHL  S    +    D F+ P++D+L ++    ++  EN+ ++ S   
Sbjct: 89  FFHGHLLPLHLLSHLPVSPRTSTGSYNDGFTLPVKDILPDQPTNNNNNTENAITNISTEA 148

Query: 143 DDHCISFDKDDNEIKCNIDFSSHHKCIKKERSSNNKSKSFSLFGLSKWKKPSEGTEKSQV 202
                   KDDN         +  K   + R      KSFSLFGLSKW+K  E  E+ Q 
Sbjct: 149 --------KDDN---------TEDKAEGEIRVKTKPIKSFSLFGLSKWRKGFESNEREQE 208

Query: 203 IENCSKRNKVKFDVTQFLKRYINMVKPLLSPKSGGRRDNVRGRRQSYSFSGTVT-PRDN- 262
            +    +  +  D++  +K+YI M   L   +  G +   R +  SYSFS ++  P  N 
Sbjct: 209 QQQQKIKKPMSLDLSHAVKKYIRM---LFQKRGNGTQFWNRRQTSSYSFSSSLMGPNGNS 268

Query: 263 ----------REIIRGRRGE-FSAPASMRTSPTNSG-LLLATAGVPSPV-------SEST 317
                     R++IRGRRGE FSAPASMRTSPTNSG L ++TAG+ S         S+ST
Sbjct: 269 KTMINGSYNKRDLIRGRRGELFSAPASMRTSPTNSGHLRVSTAGLSSSSGSTSSSSSDST 325

[Close]

BLAST of Spo08745.1 vs. ExPASy Swiss-Prot
Match: BKI1_ORYSI (Probable BRI1 kinase inhibitor 1 OS=Oryza sativa subsp. indica GN=BKI1 PE=3 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 1.300e-14
Identity = 69/169 (40.83%), Postives = 94/169 (55.62%), Query Frame = 1


		  
Query: 160 SSHHKCIKKERSSNNKSKSFSLFGLSKWKKPSEGTEKSQVIENCSKRNKVKFDVTQFLKR 219
           SS     +K+R    KSK+ S F          G  + + ++  +K      DV Q++K+
Sbjct: 95  SSSSSSKEKDREYKAKSKASSPFFSGLGGSWRSGLSRDEEVKRKAKAKTRGLDVGQWVKK 154

Query: 220 YI-NMVKPLLSPKS--GG----RRDNVRGRRQSYSFSGTVTPRDNREIIRGRRGEFS-AP 279
           Y+ +MV+ LL+  S  GG    +R+  R R  S+S  G    R+ RE  R RRG+ S AP
Sbjct: 155 YMASMVEHLLASFSRHGGGEREKREQQRRRPHSFSAHGPSALREQRERWRRRRGQLSSAP 214

Query: 280 ASMRTSPTNSGLLLATAGVPSPVS--ESTMEDLQAAIQAAIAHCKNSIS 319
           AS+R SP NSG L     V    S  ESTME+LQ+AI+AAIAHCKNSI+
Sbjct: 215 ASLRASPVNSGHLSVGGSVKVSTSSEESTMEELQSAIEAAIAHCKNSIT 263

[Close]

BLAST of Spo08745.1 vs. ExPASy Swiss-Prot
Match: BKI1_ORYSJ (Probable BRI1 kinase inhibitor 1 OS=Oryza sativa subsp. japonica GN=BKI1 PE=1 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 1.700e-14
Identity = 67/162 (41.36%), Postives = 92/162 (56.79%), Query Frame = 1


		  
Query: 167 KKERSSNNKSKSFSLFGLSKWKKPSEGTEKSQVIENCSKRNKVKFDVTQFLKRYI-NMVK 226
           +K+R    KSK+ S F          G  + + ++  +K      DV Q++K+Y+ +MV+
Sbjct: 100 EKDREYKAKSKASSPFFSGLGGSWRSGLSRDEEVKRKAKAKTRGLDVGQWVKKYMASMVE 159

Query: 227 PLLSPKS--GG----RRDNVRGRRQSYSFSGTVTPRDNREIIRGRRGEFS-APASMRTSP 286
            LL+  S  GG    +R+  R R  S+S  G    R+ RE  R RRG+ S APAS+R SP
Sbjct: 160 HLLASFSRHGGGEREKREQQRRRPHSFSAHGPSALREQRERWRRRRGQLSSAPASLRASP 219

Query: 287 TNSGLLLATAGVPSPVS--ESTMEDLQAAIQAAIAHCKNSIS 319
            NSG L     V    S  ESTME+LQ+AI+AAIAHCKNSI+
Sbjct: 220 VNSGHLSVGGSVKVSTSSEESTMEELQSAIEAAIAHCKNSIT 261

[Close]

BLAST of Spo08745.1 vs. TAIR (Arabidopsis)
Match: AT5G42750.1 (BRI1 kinase inhibitor 1)

HSP 1 Score: 185.3 bits (469), Expect = 6.300e-47
Identity = 140/318 (44.03%), Postives = 180/318 (56.60%), Query Frame = 1


		  
Query: 23  PVLSASSSPSHEFSFTISLD---SSVKTTTKASLSPSVTEKTKQQQQGPLAIDLSPADEI 82
           P+ S  SSPSH+FSFTISL    SS K  +    SPS T  + QQ   P A+DLSPADEI
Sbjct: 29  PISSTCSSPSHDFSFTISLQPLSSSSKHISPTLRSPSKTTSSYQQTD-PFAVDLSPADEI 88

Query: 83  FFHGHLLPLHFLSHLHASSARPSIHSLDSFSGPIQDVLQEEKPLTSSTIENSSSSCSCTE 142
           FFHGHLLPLH LSHL  S    +    D F+ P++D+L ++    ++  EN+ ++ S   
Sbjct: 89  FFHGHLLPLHLLSHLPVSPRTSTGSYNDGFTLPVKDILPDQPTNNNNNTENAITNISTEA 148

Query: 143 DDHCISFDKDDNEIKCNIDFSSHHKCIKKERSSNNKSKSFSLFGLSKWKKPSEGTEKSQV 202
                   KDDN         +  K   + R      KSFSLFGLSKW+K  E  E+ Q 
Sbjct: 149 --------KDDN---------TEDKAEGEIRVKTKPIKSFSLFGLSKWRKGFESNEREQE 208

Query: 203 IENCSKRNKVKFDVTQFLKRYINMVKPLLSPKSGGRRDNVRGRRQSYSFSGTVT-PRDN- 262
            +    +  +  D++  +K+YI M   L   +  G +   R +  SYSFS ++  P  N 
Sbjct: 209 QQQQKIKKPMSLDLSHAVKKYIRM---LFQKRGNGTQFWNRRQTSSYSFSSSLMGPNGNS 268

Query: 263 ----------REIIRGRRGE-FSAPASMRTSPTNSG-LLLATAGVPSPV-------SEST 317
                     R++IRGRRGE FSAPASMRTSPTNSG L ++TAG+ S         S+ST
Sbjct: 269 KTMINGSYNKRDLIRGRRGELFSAPASMRTSPTNSGHLRVSTAGLSSSSGSTSSSSSDST 325

The following BLAST results are available for this feature:

BLAST of Spo08745.1 vs. NCBI nr
BLAST of Spo08745.1 vs. UniProtKB/TrEMBL
BLAST of Spo08745.1 vs. ExPASy Swiss-Prot
BLAST of Spo08745.1 vs. TAIR (Arabidopsis)

BLAST of Spo08745.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902220225\|gb\|KNA18535.1\|	1.2e-180	100.	hypothetical protein SOVF_0698...	[more]
gi\|731362252\|ref\|XP_010692794.1\|	1.7e-94	63.3	PREDICTED: BRI1 kinase inhibit...	[more]
gi\|225458591\|ref\|XP_002284646.1\|	2.2e-62	54.6	PREDICTED: BRI1 kinase inhibit...	[more]
gi\|590592448\|ref\|XP_007017284.1\|	3.8e-62	52.2	BRI1 kinase inhibitor 1 [Theob...	[more]
gi\|147866713\|emb\|CAN78408.1\|	5.0e-62	54.2	hypothetical protein VITISV_02...	[more]

BLAST of Spo08745.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9RGD0_SPIOL	8.3e-181	100.	Uncharacterized protein OS=Spi...	[more]
A0A0J8E8Z9_BETVU	1.2e-94	63.3	Uncharacterized protein OS=Bet...	[more]
F6H005_VITVI	1.6e-62	54.6	Putative uncharacterized prote...	[more]
A0A061FJ36_THECC	2.7e-62	52.2	BRI1 kinase inhibitor 1 OS=The...	[more]
A5AWQ1_VITVI	3.5e-62	54.2	Putative uncharacterized prote...	[more]

BLAST of Spo08745.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 3


Match Name	E-value	Identity	Description
BKI1_ARATH	1.1e-45	44.0	BRI1 kinase inhibitor 1 OS=Ara...	[more]
BKI1_ORYSI	1.3e-14	40.8	Probable BRI1 kinase inhibitor...	[more]
BKI1_ORYSJ	1.7e-14	41.3	Probable BRI1 kinase inhibitor...	[more]

BLAST of Spo08745.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 1


Match Name	E-value	Identity	Description
AT5G42750.1	6.3e-47	44.0	BRI1 kinase inhibitor 1	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR33312	FAMILY NOT NAMED	coord: 69..324 score: 7.5E-88coord: 18..51 score: 7.5
None	No IPR available	PANTHER	PTHR33312:SF8	BRI1 KINASE INHIBITOR 1	coord: 18..51 score: 7.5E-88coord: 69..324 score: 7.5

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009742	brassinosteroid mediated signaling pathway
biological_process	GO:0010423	negative regulation of brassinosteroid biosynthetic process
cellular_component	GO:0005829	cytosol
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0046982	protein heterodimerization activity