Spo09366.1 (mRNA) | SpinachBase

Overview


Name	Spo09366.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	Vacuolar protein sorting-associated protein VPS28 family protein
Location	SpoScf_01466 : 7640 .. 8269 (+)
Sequence length	630

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGTGAAACTATGGAATGATAAGCGTGAAAGAGAAATGTATGAGAATTTTGCCGAGCTTTTCGCCATTATTAAAGCTACTGAGAAGCTTGAAAAGGCTTACATTAGGGATGTTATTGCACCTTCAGACTATGAAACTGAGTGCCTTAAACTGATTGCCCACTTCAAAACCCTTTCATCATCGTTGAAAGACACTGTCCCAAGCATTGAGAGCTTTGCTGATACATATAAAATGGATTGCCGGGCCGCGTTAAATAGGCTGGTAACTTCTGGGGTTCCTGCTACAGTGGAACACAGATCAGCTGCTGCTTCTTCTTCCACAAATTCAGCTGCCACTGTTGCAGAGTGTGTTCAGAACTTCATCACATCCATGGACTCATTGAAATTGAACATGGTGGCGGTTGATCAGGTTTATCCCCTGCTTTCGGACCTCTCAACGTCGTTGAACAAGCTCAGTTTATTGCCGCCTGATTTTGAAGGGAAGACCAAAATGAAAGAGTGGCTCGGACGGTTGTCTAAGATGGGAGCTTCTGATGAGCTGACTGAGCAACAAGCAAGACAGCTTCACTTTGATCTCGAGTCTTCGTACAACTCTTTCATGGCTGCGTTGCCTCATGCTGGAAATTGA

mRNA sequence

ATGGAAGTGAAACTATGGAATGATAAGCGTGAAAGAGAAATGTATGAGAATTTTGCCGAGCTTTTCGCCATTATTAAAGCTACTGAGAAGCTTGAAAAGGCTTACATTAGGGATGTTATTGCACCTTCAGACTATGAAACTGAGTGCCTTAAACTGATTGCCCACTTCAAAACCCTTTCATCATCGTTGAAAGACACTGTCCCAAGCATTGAGAGCTTTGCTGATACATATAAAATGGATTGCCGGGCCGCGTTAAATAGGCTGGTAACTTCTGGGGTTCCTGCTACAGTGGAACACAGATCAGCTGCTGCTTCTTCTTCCACAAATTCAGCTGCCACTGTTGCAGAGTGTGTTCAGAACTTCATCACATCCATGGACTCATTGAAATTGAACATGGTGGCGGTTGATCAGGTTTATCCCCTGCTTTCGGACCTCTCAACGTCGTTGAACAAGCTCAGTTTATTGCCGCCTGATTTTGAAGGGAAGACCAAAATGAAAGAGTGGCTCGGACGGTTGTCTAAGATGGGAGCTTCTGATGAGCTGACTGAGCAACAAGCAAGACAGCTTCACTTTGATCTCGAGTCTTCGTACAACTCTTTCATGGCTGCGTTGCCTCATGCTGGAAATTGA

Coding sequence (CDS)

ATGGAAGTGAAACTATGGAATGATAAGCGTGAAAGAGAAATGTATGAGAATTTTGCCGAGCTTTTCGCCATTATTAAAGCTACTGAGAAGCTTGAAAAGGCTTACATTAGGGATGTTATTGCACCTTCAGACTATGAAACTGAGTGCCTTAAACTGATTGCCCACTTCAAAACCCTTTCATCATCGTTGAAAGACACTGTCCCAAGCATTGAGAGCTTTGCTGATACATATAAAATGGATTGCCGGGCCGCGTTAAATAGGCTGGTAACTTCTGGGGTTCCTGCTACAGTGGAACACAGATCAGCTGCTGCTTCTTCTTCCACAAATTCAGCTGCCACTGTTGCAGAGTGTGTTCAGAACTTCATCACATCCATGGACTCATTGAAATTGAACATGGTGGCGGTTGATCAGGTTTATCCCCTGCTTTCGGACCTCTCAACGTCGTTGAACAAGCTCAGTTTATTGCCGCCTGATTTTGAAGGGAAGACCAAAATGAAAGAGTGGCTCGGACGGTTGTCTAAGATGGGAGCTTCTGATGAGCTGACTGAGCAACAAGCAAGACAGCTTCACTTTGATCTCGAGTCTTCGTACAACTCTTTCATGGCTGCGTTGCCTCATGCTGGAAATTGA

Protein sequence

MEVKLWNDKREREMYENFAELFAIIKATEKLEKAYIRDVIAPSDYETECLKLIAHFKTLSSSLKDTVPSIESFADTYKMDCRAALNRLVTSGVPATVEHRSAAASSSTNSAATVAECVQNFITSMDSLKLNMVAVDQVYPLLSDLSTSLNKLSLLPPDFEGKTKMKEWLGRLSKMGASDELTEQQARQLHFDLESSYNSFMAALPHAGN

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo09366	Spo09366	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo09366.1	Spo09366.1-protein	polypeptide

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo09366.1.exon.1	Spo09366.1.exon.1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo09366.1.CDS.1	Spo09366.1.CDS.1	CDS

Homology

[Close]

BLAST of Spo09366.1 vs. NCBI nr
Match: gi|902184823|gb|KNA10377.1| (hypothetical protein SOVF_144800 [Spinacia oleracea])

HSP 1 Score: 401.4 bits (1030), Expect = 9.900e-109
Identity = 209/209 (100.00%), Postives = 209/209 (100.00%), Query Frame = 1


		  
Query: 1   MEVKLWNDKREREMYENFAELFAIIKATEKLEKAYIRDVIAPSDYETECLKLIAHFKTLS 60
           MEVKLWNDKREREMYENFAELFAIIKATEKLEKAYIRDVIAPSDYETECLKLIAHFKTLS
Sbjct: 1   MEVKLWNDKREREMYENFAELFAIIKATEKLEKAYIRDVIAPSDYETECLKLIAHFKTLS 60

Query: 61  SSLKDTVPSIESFADTYKMDCRAALNRLVTSGVPATVEHRSAAASSSTNSAATVAECVQN 120
           SSLKDTVPSIESFADTYKMDCRAALNRLVTSGVPATVEHRSAAASSSTNSAATVAECVQN
Sbjct: 61  SSLKDTVPSIESFADTYKMDCRAALNRLVTSGVPATVEHRSAAASSSTNSAATVAECVQN 120

Query: 121 FITSMDSLKLNMVAVDQVYPLLSDLSTSLNKLSLLPPDFEGKTKMKEWLGRLSKMGASDE 180
           FITSMDSLKLNMVAVDQVYPLLSDLSTSLNKLSLLPPDFEGKTKMKEWLGRLSKMGASDE
Sbjct: 121 FITSMDSLKLNMVAVDQVYPLLSDLSTSLNKLSLLPPDFEGKTKMKEWLGRLSKMGASDE 180

Query: 181 LTEQQARQLHFDLESSYNSFMAALPHAGN 210
           LTEQQARQLHFDLESSYNSFMAALPHAGN
Sbjct: 181 LTEQQARQLHFDLESSYNSFMAALPHAGN 209

[Close]

BLAST of Spo09366.1 vs. NCBI nr
Match: gi|731369614|ref|XP_010696614.1| (PREDICTED: vacuolar protein sorting-associated protein 28 homolog 2 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 379.4 bits (973), Expect = 4.000e-102
Identity = 195/209 (93.30%), Postives = 202/209 (96.65%), Query Frame = 1


		  
Query: 1   MEVKLWNDKREREMYENFAELFAIIKATEKLEKAYIRDVIAPSDYETECLKLIAHFKTLS 60
           MEVKLWNDKREREMYENFAELFAIIKATEKLEKAYIRD+I+PSDYE ECLKLIAHFKTLS
Sbjct: 1   MEVKLWNDKREREMYENFAELFAIIKATEKLEKAYIRDIISPSDYENECLKLIAHFKTLS 60

Query: 61  SSLKDTVPSIESFADTYKMDCRAALNRLVTSGVPATVEHRSAAASSSTNSAATVAECVQN 120
           SSLKDTVPSIE F D YKMDC AALNRLVTSGVPATVEHR+A+A+SST SAATVAECVQN
Sbjct: 61  SSLKDTVPSIEKFVDVYKMDCSAALNRLVTSGVPATVEHRAASAASSTTSAATVAECVQN 120

Query: 121 FITSMDSLKLNMVAVDQVYPLLSDLSTSLNKLSLLPPDFEGKTKMKEWLGRLSKMGASDE 180
           FITSMDSLKLNMVAVDQVYPLLSDLSTSLNKLS+LPPDFEGKTKMKEWLGRLSKMGA+DE
Sbjct: 121 FITSMDSLKLNMVAVDQVYPLLSDLSTSLNKLSILPPDFEGKTKMKEWLGRLSKMGAADE 180

Query: 181 LTEQQARQLHFDLESSYNSFMAALPHAGN 210
           LTEQQARQLHFDLESSYNSFMAALP AGN
Sbjct: 181 LTEQQARQLHFDLESSYNSFMAALPSAGN 209

[Close]

BLAST of Spo09366.1 vs. NCBI nr
Match: gi|731354719|ref|XP_010688749.1| (PREDICTED: vacuolar protein sorting-associated protein 28 homolog 1-like [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 376.7 bits (966), Expect = 2.600e-101
Identity = 193/209 (92.34%), Postives = 201/209 (96.17%), Query Frame = 1


		  
Query: 1   MEVKLWNDKREREMYENFAELFAIIKATEKLEKAYIRDVIAPSDYETECLKLIAHFKTLS 60
           MEVKLWNDK EREMY+NFAELFAIIKATEKLEKAYIRD+I+PSDYETEC KLIAHFKTLS
Sbjct: 1   MEVKLWNDKHEREMYDNFAELFAIIKATEKLEKAYIRDIISPSDYETECFKLIAHFKTLS 60

Query: 61  SSLKDTVPSIESFADTYKMDCRAALNRLVTSGVPATVEHRSAAASSSTNSAATVAECVQN 120
           SSLKDTVPSIE F D YKMDC AALNRLVTSGVPATVEHR+A+A+SST SAATVAECVQN
Sbjct: 61  SSLKDTVPSIEKFVDVYKMDCSAALNRLVTSGVPATVEHRAASAASSTTSAATVAECVQN 120

Query: 121 FITSMDSLKLNMVAVDQVYPLLSDLSTSLNKLSLLPPDFEGKTKMKEWLGRLSKMGASDE 180
           FITSMDSLKLNMVAVDQVYPLLSDLSTSLNKLS+LPPDFEGKTKMKEWLGRLSKMGA+DE
Sbjct: 121 FITSMDSLKLNMVAVDQVYPLLSDLSTSLNKLSILPPDFEGKTKMKEWLGRLSKMGAADE 180

Query: 181 LTEQQARQLHFDLESSYNSFMAALPHAGN 210
           LTEQQARQLHFDLESSYNSFMAALP AGN
Sbjct: 181 LTEQQARQLHFDLESSYNSFMAALPSAGN 209

[Close]

BLAST of Spo09366.1 vs. NCBI nr
Match: gi|590625041|ref|XP_007025773.1| (Vacuolar protein sorting-associated protein VPS28 family protein [Theobroma cacao])

HSP 1 Score: 360.5 bits (924), Expect = 1.900e-96
Identity = 183/209 (87.56%), Postives = 200/209 (95.69%), Query Frame = 1


		  
Query: 1   MEVKLWNDKREREMYENFAELFAIIKATEKLEKAYIRDVIAPSDYETECLKLIAHFKTLS 60
           MEVKLWNDKREREMYENFAEL+AIIKATEKLEKAY+RD+I  S+YETEC KLIAHFKTL+
Sbjct: 1   MEVKLWNDKREREMYENFAELYAIIKATEKLEKAYVRDIITSSEYETECQKLIAHFKTLA 60

Query: 61  SSLKDTVPSIESFADTYKMDCRAALNRLVTSGVPATVEHRSAAASSSTNSAATVAECVQN 120
           S+LKDTVPSIE FADTYKMDC AA+NRLVTSGVPATVEHR+AAA+S+T SAA VAECVQN
Sbjct: 61  STLKDTVPSIERFADTYKMDCPAAINRLVTSGVPATVEHRAAAAASATTSAAIVAECVQN 120

Query: 121 FITSMDSLKLNMVAVDQVYPLLSDLSTSLNKLSLLPPDFEGKTKMKEWLGRLSKMGASDE 180
           FIT+MDSLKLNMVAVDQV+PLLSDLS SLNKLS+LPPDFEGKTKMKEW+ RLSKMGA+DE
Sbjct: 121 FITAMDSLKLNMVAVDQVHPLLSDLSASLNKLSILPPDFEGKTKMKEWISRLSKMGAADE 180

Query: 181 LTEQQARQLHFDLESSYNSFMAALPHAGN 210
           LTEQQARQLHFDLESSYNSFMAALP+AG+
Sbjct: 181 LTEQQARQLHFDLESSYNSFMAALPNAGS 209

[Close]

BLAST of Spo09366.1 vs. NCBI nr
Match: gi|659078771|ref|XP_008439898.1| (PREDICTED: vacuolar protein sorting-associated protein 28 homolog 2 [Cucumis melo])

HSP 1 Score: 358.2 bits (918), Expect = 9.600e-96
Identity = 182/208 (87.50%), Postives = 199/208 (95.67%), Query Frame = 1


		  
Query: 1   MEVKLWNDKREREMYENFAELFAIIKATEKLEKAYIRDVIAPSDYETECLKLIAHFKTLS 60
           MEVKLWNDKREREMY+NFAEL+AIIKATEKLEKAYIRD+I+ SDYETEC KLIAHFKTL+
Sbjct: 1   MEVKLWNDKREREMYDNFAELYAIIKATEKLEKAYIRDIISSSDYETECFKLIAHFKTLA 60

Query: 61  SSLKDTVPSIESFADTYKMDCRAALNRLVTSGVPATVEHRSAAASSSTNSAATVAECVQN 120
           S+LKDTVPSIE FADTYKMDC AA+NRLV +GVPATVEHR+AAA+S+T SAA VAECVQN
Sbjct: 61  STLKDTVPSIERFADTYKMDCPAAINRLVITGVPATVEHRAAAAASATTSAAIVAECVQN 120

Query: 121 FITSMDSLKLNMVAVDQVYPLLSDLSTSLNKLSLLPPDFEGKTKMKEWLGRLSKMGASDE 180
           FIT+MDSLKLNMVAVDQV+PLLSDL+ SLNKL++LPPDFEGKTKMKEWLGRLSKMGA+DE
Sbjct: 121 FITAMDSLKLNMVAVDQVHPLLSDLAASLNKLTILPPDFEGKTKMKEWLGRLSKMGAADE 180

Query: 181 LTEQQARQLHFDLESSYNSFMAALPHAG 209
           LTEQQARQLHFDLESSYNSFMAALP AG
Sbjct: 181 LTEQQARQLHFDLESSYNSFMAALPTAG 208

[Close]

BLAST of Spo09366.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QSV0_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_144800 PE=4 SV=1)

HSP 1 Score: 401.4 bits (1030), Expect = 6.900e-109
Identity = 209/209 (100.00%), Postives = 209/209 (100.00%), Query Frame = 1


		  
Query: 1   MEVKLWNDKREREMYENFAELFAIIKATEKLEKAYIRDVIAPSDYETECLKLIAHFKTLS 60
           MEVKLWNDKREREMYENFAELFAIIKATEKLEKAYIRDVIAPSDYETECLKLIAHFKTLS
Sbjct: 1   MEVKLWNDKREREMYENFAELFAIIKATEKLEKAYIRDVIAPSDYETECLKLIAHFKTLS 60

Query: 61  SSLKDTVPSIESFADTYKMDCRAALNRLVTSGVPATVEHRSAAASSSTNSAATVAECVQN 120
           SSLKDTVPSIESFADTYKMDCRAALNRLVTSGVPATVEHRSAAASSSTNSAATVAECVQN
Sbjct: 61  SSLKDTVPSIESFADTYKMDCRAALNRLVTSGVPATVEHRSAAASSSTNSAATVAECVQN 120

Query: 121 FITSMDSLKLNMVAVDQVYPLLSDLSTSLNKLSLLPPDFEGKTKMKEWLGRLSKMGASDE 180
           FITSMDSLKLNMVAVDQVYPLLSDLSTSLNKLSLLPPDFEGKTKMKEWLGRLSKMGASDE
Sbjct: 121 FITSMDSLKLNMVAVDQVYPLLSDLSTSLNKLSLLPPDFEGKTKMKEWLGRLSKMGASDE 180

Query: 181 LTEQQARQLHFDLESSYNSFMAALPHAGN 210
           LTEQQARQLHFDLESSYNSFMAALPHAGN
Sbjct: 181 LTEQQARQLHFDLESSYNSFMAALPHAGN 209

[Close]

BLAST of Spo09366.1 vs. UniProtKB/TrEMBL
Match: A0A0J8B717_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_8g199790 PE=4 SV=1)

HSP 1 Score: 379.4 bits (973), Expect = 2.800e-102
Identity = 195/209 (93.30%), Postives = 202/209 (96.65%), Query Frame = 1


		  
Query: 1   MEVKLWNDKREREMYENFAELFAIIKATEKLEKAYIRDVIAPSDYETECLKLIAHFKTLS 60
           MEVKLWNDKREREMYENFAELFAIIKATEKLEKAYIRD+I+PSDYE ECLKLIAHFKTLS
Sbjct: 1   MEVKLWNDKREREMYENFAELFAIIKATEKLEKAYIRDIISPSDYENECLKLIAHFKTLS 60

Query: 61  SSLKDTVPSIESFADTYKMDCRAALNRLVTSGVPATVEHRSAAASSSTNSAATVAECVQN 120
           SSLKDTVPSIE F D YKMDC AALNRLVTSGVPATVEHR+A+A+SST SAATVAECVQN
Sbjct: 61  SSLKDTVPSIEKFVDVYKMDCSAALNRLVTSGVPATVEHRAASAASSTTSAATVAECVQN 120

Query: 121 FITSMDSLKLNMVAVDQVYPLLSDLSTSLNKLSLLPPDFEGKTKMKEWLGRLSKMGASDE 180
           FITSMDSLKLNMVAVDQVYPLLSDLSTSLNKLS+LPPDFEGKTKMKEWLGRLSKMGA+DE
Sbjct: 121 FITSMDSLKLNMVAVDQVYPLLSDLSTSLNKLSILPPDFEGKTKMKEWLGRLSKMGAADE 180

Query: 181 LTEQQARQLHFDLESSYNSFMAALPHAGN 210
           LTEQQARQLHFDLESSYNSFMAALP AGN
Sbjct: 181 LTEQQARQLHFDLESSYNSFMAALPSAGN 209

[Close]

BLAST of Spo09366.1 vs. UniProtKB/TrEMBL
Match: A0A0J8BMT2_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_9g203370 PE=4 SV=1)

HSP 1 Score: 376.7 bits (966), Expect = 1.800e-101
Identity = 193/209 (92.34%), Postives = 201/209 (96.17%), Query Frame = 1


		  
Query: 1   MEVKLWNDKREREMYENFAELFAIIKATEKLEKAYIRDVIAPSDYETECLKLIAHFKTLS 60
           MEVKLWNDK EREMY+NFAELFAIIKATEKLEKAYIRD+I+PSDYETEC KLIAHFKTLS
Sbjct: 1   MEVKLWNDKHEREMYDNFAELFAIIKATEKLEKAYIRDIISPSDYETECFKLIAHFKTLS 60

Query: 61  SSLKDTVPSIESFADTYKMDCRAALNRLVTSGVPATVEHRSAAASSSTNSAATVAECVQN 120
           SSLKDTVPSIE F D YKMDC AALNRLVTSGVPATVEHR+A+A+SST SAATVAECVQN
Sbjct: 61  SSLKDTVPSIEKFVDVYKMDCSAALNRLVTSGVPATVEHRAASAASSTTSAATVAECVQN 120

Query: 121 FITSMDSLKLNMVAVDQVYPLLSDLSTSLNKLSLLPPDFEGKTKMKEWLGRLSKMGASDE 180
           FITSMDSLKLNMVAVDQVYPLLSDLSTSLNKLS+LPPDFEGKTKMKEWLGRLSKMGA+DE
Sbjct: 121 FITSMDSLKLNMVAVDQVYPLLSDLSTSLNKLSILPPDFEGKTKMKEWLGRLSKMGAADE 180

Query: 181 LTEQQARQLHFDLESSYNSFMAALPHAGN 210
           LTEQQARQLHFDLESSYNSFMAALP AGN
Sbjct: 181 LTEQQARQLHFDLESSYNSFMAALPSAGN 209

[Close]

BLAST of Spo09366.1 vs. UniProtKB/TrEMBL
Match: A0A061GEU0_THECC (Vacuolar protein sorting-associated protein VPS28 family protein OS=Theobroma cacao GN=TCM_029974 PE=4 SV=1)

HSP 1 Score: 360.5 bits (924), Expect = 1.300e-96
Identity = 183/209 (87.56%), Postives = 200/209 (95.69%), Query Frame = 1


		  
Query: 1   MEVKLWNDKREREMYENFAELFAIIKATEKLEKAYIRDVIAPSDYETECLKLIAHFKTLS 60
           MEVKLWNDKREREMYENFAEL+AIIKATEKLEKAY+RD+I  S+YETEC KLIAHFKTL+
Sbjct: 1   MEVKLWNDKREREMYENFAELYAIIKATEKLEKAYVRDIITSSEYETECQKLIAHFKTLA 60

Query: 61  SSLKDTVPSIESFADTYKMDCRAALNRLVTSGVPATVEHRSAAASSSTNSAATVAECVQN 120
           S+LKDTVPSIE FADTYKMDC AA+NRLVTSGVPATVEHR+AAA+S+T SAA VAECVQN
Sbjct: 61  STLKDTVPSIERFADTYKMDCPAAINRLVTSGVPATVEHRAAAAASATTSAAIVAECVQN 120

Query: 121 FITSMDSLKLNMVAVDQVYPLLSDLSTSLNKLSLLPPDFEGKTKMKEWLGRLSKMGASDE 180
           FIT+MDSLKLNMVAVDQV+PLLSDLS SLNKLS+LPPDFEGKTKMKEW+ RLSKMGA+DE
Sbjct: 121 FITAMDSLKLNMVAVDQVHPLLSDLSASLNKLSILPPDFEGKTKMKEWISRLSKMGAADE 180

Query: 181 LTEQQARQLHFDLESSYNSFMAALPHAGN 210
           LTEQQARQLHFDLESSYNSFMAALP+AG+
Sbjct: 181 LTEQQARQLHFDLESSYNSFMAALPNAGS 209

[Close]

BLAST of Spo09366.1 vs. UniProtKB/TrEMBL
Match: B9S615_RICCO (Vacuolar protein sorting-associated protein VPS28, putative OS=Ricinus communis GN=RCOM_1063030 PE=4 SV=1)

HSP 1 Score: 357.8 bits (917), Expect = 8.700e-96
Identity = 184/208 (88.46%), Postives = 196/208 (94.23%), Query Frame = 1


		  
Query: 1   MEVKLWNDKREREMYENFAELFAIIKATEKLEKAYIRDVIAPSDYETECLKLIAHFKTLS 60
           MEVKLWNDKREREMYENFAEL+AIIKATEKLEKAYIRD+I+ SDYETEC KLIAHFKTL+
Sbjct: 1   MEVKLWNDKREREMYENFAELYAIIKATEKLEKAYIRDIISSSDYETECQKLIAHFKTLA 60

Query: 61  SSLKDTVPSIESFADTYKMDCRAALNRLVTSGVPATVEHRSAAASSSTNSAATVAECVQN 120
           S+LKD VPSIE FADTYKMDC AA+NRLVTSGVPATVEHR+AAASS+T SAA VAECVQN
Sbjct: 61  STLKDIVPSIERFADTYKMDCPAAINRLVTSGVPATVEHRAAAASSATTSAAIVAECVQN 120

Query: 121 FITSMDSLKLNMVAVDQVYPLLSDLSTSLNKLSLLPPDFEGKTKMKEWLGRLSKMGASDE 180
           FIT+MDSLKLNMVAVDQVYPLLSDLS SLNKLS+LPPDFEGK KMK+W+ RLSKMGASDE
Sbjct: 121 FITAMDSLKLNMVAVDQVYPLLSDLSASLNKLSILPPDFEGKLKMKDWITRLSKMGASDE 180

Query: 181 LTEQQARQLHFDLESSYNSFMAALPHAG 209
           LTEQQARQLHFDLESSYNSFMA LP AG
Sbjct: 181 LTEQQARQLHFDLESSYNSFMAVLPSAG 208

[Close]

BLAST of Spo09366.1 vs. ExPASy Swiss-Prot
Match: VP282_ARATH (Vacuolar protein sorting-associated protein 28 homolog 2 OS=Arabidopsis thaliana GN=VPS28-2 PE=1 SV=2)

HSP 1 Score: 356.3 bits (913), Expect = 2.300e-97
Identity = 182/209 (87.08%), Postives = 197/209 (94.26%), Query Frame = 1


		  
Query: 1   MEVKLWNDKREREMYENFAELFAIIKATEKLEKAYIRDVIAPSDYETECLKLIAHFKTLS 60
           MEVKLWNDKREREMYENFAELFAIIKATEKLEKAYIRD+I PS+YE+EC KLI HFKTLS
Sbjct: 2   MEVKLWNDKREREMYENFAELFAIIKATEKLEKAYIRDLINPSEYESECQKLIVHFKTLS 61

Query: 61  SSLKDTVPSIESFADTYKMDCRAALNRLVTSGVPATVEHRSAAASSSTNSAATVAECVQN 120
           ++LKDTVP+IE FADTYKMDC AAL RLVTSG+PATVEHR+  A+S++NSA+ VAECVQN
Sbjct: 62  ATLKDTVPNIERFADTYKMDCPAALYRLVTSGLPATVEHRATVAASTSNSASIVAECVQN 121

Query: 121 FITSMDSLKLNMVAVDQVYPLLSDLSTSLNKLSLLPPDFEGKTKMKEWLGRLSKMGASDE 180
           FITSMDSLKLNMVAVDQVYPLLSDLS SLNKLS+LPPDFEGKTKMKEWL RLSKMGA+DE
Sbjct: 122 FITSMDSLKLNMVAVDQVYPLLSDLSASLNKLSILPPDFEGKTKMKEWLSRLSKMGAADE 181

Query: 181 LTEQQARQLHFDLESSYNSFMAALPHAGN 210
           LTEQQ+RQLHFDLESSYNSFMAALP AGN
Sbjct: 182 LTEQQSRQLHFDLESSYNSFMAALPKAGN 210

[Close]

BLAST of Spo09366.1 vs. ExPASy Swiss-Prot
Match: VP281_ARATH (Vacuolar protein sorting-associated protein 28 homolog 1 OS=Arabidopsis thaliana GN=VPS28-1 PE=1 SV=1)

HSP 1 Score: 355.1 bits (910), Expect = 5.100e-97
Identity = 182/209 (87.08%), Postives = 199/209 (95.22%), Query Frame = 1


		  
Query: 1   MEVKLWNDKREREMYENFAELFAIIKATEKLEKAYIRDVIAPSDYETECLKLIAHFKTLS 60
           MEVKLWNDKREREMYENFAEL+AIIKATEKLEKAYIRD+I+PS+YETEC KLI HFKTLS
Sbjct: 1   MEVKLWNDKREREMYENFAELYAIIKATEKLEKAYIRDLISPSEYETECQKLIVHFKTLS 60

Query: 61  SSLKDTVPSIESFADTYKMDCRAALNRLVTSGVPATVEHRSAAASSSTNSAATVAECVQN 120
           +SLKD VP+IE FA+TYKMDC AA+ RLVTSGVPATVEHR+AA++S+++SA+ VAECVQN
Sbjct: 61  ASLKDMVPNIERFAETYKMDCSAAVYRLVTSGVPATVEHRAAASASTSSSASVVAECVQN 120

Query: 121 FITSMDSLKLNMVAVDQVYPLLSDLSTSLNKLSLLPPDFEGKTKMKEWLGRLSKMGASDE 180
           FITSMDSLKLNMVAVDQVYPLLSDLS SLNKLS+LPPDFEGK KMKEWL RLSKMGASDE
Sbjct: 121 FITSMDSLKLNMVAVDQVYPLLSDLSASLNKLSILPPDFEGKIKMKEWLLRLSKMGASDE 180

Query: 181 LTEQQARQLHFDLESSYNSFMAALPHAGN 210
           LTEQQARQLHFDLESSYNSFMAALP+AGN
Sbjct: 181 LTEQQARQLHFDLESSYNSFMAALPNAGN 209

[Close]

BLAST of Spo09366.1 vs. ExPASy Swiss-Prot
Match: VPS28_DICDI (Vacuolar protein sorting-associated protein 28 OS=Dictyostelium discoideum GN=vps28 PE=3 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 7.000e-54
Identity = 111/203 (54.68%), Postives = 147/203 (72.41%), Query Frame = 1


		  
Query: 2   EVKLWNDKREREMYENFAELFAIIKATEKLEKAYIRDVIAPSDYETECLKLIAHFKTLSS 61
           EVKL+N+  EREMYEN AEL++IIK TE LEKAYIRD ++P DY T C KLIA FK+  +
Sbjct: 84  EVKLFNNNIEREMYENLAELYSIIKVTEHLEKAYIRDDVSPKDYTTACSKLIAQFKSSQT 143

Query: 62  SLKDTVPSIESFADTYKMDCRAALNRLVTSGVPATVEHRSAAASSSTNSAATVAECVQNF 121
            LKD V ++  F   Y ++C+AA +RLV  G P+T+EH +  +S+ +  A  VAE VQ F
Sbjct: 144 LLKDQVSNVGQFMKDYDLNCKAAFDRLVIKGFPSTLEHNTNESSTDSAMAKNVAEAVQLF 203

Query: 122 ITSMDSLKLNMVAVDQVYPLLSDLSTSLNKLSLLPPDFEGKTKMKEWLGRLSKMGASDEL 181
           IT+MDS++L +V+VD +YPLLSDL  SLNK   L P FEGK K+K W+  L++M A+DEL
Sbjct: 204 ITTMDSIRLKLVSVDGIYPLLSDLMESLNKNQWLGPTFEGKEKIKNWISILNQMKATDEL 263

Query: 182 TEQQARQLHFDLESSYNSFMAAL 205
            + Q+RQL FDL++SYN F  A+
Sbjct: 264 DDDQSRQLLFDLDNSYNIFYKAI 286

[Close]

BLAST of Spo09366.1 vs. ExPASy Swiss-Prot
Match: VPS28_DROME (Vacuolar protein sorting-associated protein 28 homolog OS=Drosophila melanogaster GN=Vps28 PE=1 SV=1)

HSP 1 Score: 159.1 bits (401), Expect = 5.400e-38
Identity = 87/200 (43.50%), Postives = 127/200 (63.50%), Query Frame = 1


		  
Query: 2   EVKLWNDKREREMYENFAELFAIIKATEKLEKAYIRDVIAPSDYETECLKLIAHFKTLSS 61
           EVKL+ + RERE Y+N A+L+AII   ++LEKAYIRD I P +Y   C K +  +K    
Sbjct: 11  EVKLFRNAREREKYDNMADLYAIINTIQQLEKAYIRDCITPQEYTAACSKYLVQYKVAFK 70

Query: 62  SLK-DTVPSIESFADTYKMDCRAALNRLVTSGVPATVEHRSAAASSSTNSAATVAECVQN 121
            ++ D  PS+E+F   +++DC AAL R +    P T+           N++  +AE V  
Sbjct: 71  QVQCDEFPSVETFVKKFRLDCPAALER-IREDRPITIR------DDKGNTSKCIAEIVSL 130

Query: 122 FITSMDSLKLNMVAVDQVYPLLSDLSTSLNKLSLLPPDFEGKTKMKEWLGRLSKMGASDE 181
           FIT MD L+L +  +D + P + DL+ ++N+LSL+P DF+ K K+++WLG L++M ASDE
Sbjct: 131 FITIMDKLRLQINTMDALQPDVKDLADNMNRLSLIPEDFDAKLKVEKWLGSLNEMQASDE 190

Query: 182 LTEQQARQLHFDLESSYNSF 201
           L+E Q RQ  FDLES+Y  F
Sbjct: 191 LSEGQVRQFLFDLESAYADF 203

[Close]

BLAST of Spo09366.1 vs. ExPASy Swiss-Prot
Match: VPS28_HUMAN (Vacuolar protein sorting-associated protein 28 homolog OS=Homo sapiens GN=VPS28 PE=1 SV=1)

HSP 1 Score: 153.3 bits (386), Expect = 2.900e-36
Identity = 82/200 (41.00%), Postives = 127/200 (63.50%), Query Frame = 1


		  
Query: 2   EVKLWNDKREREMYENFAELFAIIKATEKLEKAYIRDVIAPSDYETECLKLIAHFKTLSS 61
           EVKL+ + RERE Y+N AELFA++K  + LEKAYI+D ++PS+Y   C +L+  +K    
Sbjct: 23  EVKLYKNAREREKYDNMAELFAVVKTMQALEKAYIKDCVSPSEYTAACSRLLVQYKAAFR 82

Query: 62  SLKDT-VPSIESFADTYKMDCRAALNRLVTSGVPATVEHRSAAASSSTNSAATVAECVQN 121
            ++ + + SI+ F   +++DC  A+ R +    P T++          N    +A+ V  
Sbjct: 83  QVQGSEISSIDEFCRKFRLDCPLAMER-IKEDRPITIK------DDKGNLNRCIADVVSL 142

Query: 122 FITSMDSLKLNMVAVDQVYPLLSDLSTSLNKLSLLPPDFEGKTKMKEWLGRLSKMGASDE 181
           FIT MD L+L + A+D++ P L +L  +++++S LPPDFEG+  + +WL  LS M ASDE
Sbjct: 143 FITVMDKLRLEIRAMDEIQPDLRELMETMHRMSHLPPDFEGRQTVSQWLQTLSGMSASDE 202

Query: 182 LTEQQARQLHFDLESSYNSF 201
           L + Q RQ+ FDLES+YN+F
Sbjct: 203 LDDSQVRQMLFDLESAYNAF 215

[Close]

BLAST of Spo09366.1 vs. TAIR (Arabidopsis)
Match: AT4G05000.1 (Vacuolar protein sorting-associated protein VPS28 family protein)

HSP 1 Score: 356.3 bits (913), Expect = 1.300e-98
Identity = 182/209 (87.08%), Postives = 197/209 (94.26%), Query Frame = 1


		  
Query: 1   MEVKLWNDKREREMYENFAELFAIIKATEKLEKAYIRDVIAPSDYETECLKLIAHFKTLS 60
           MEVKLWNDKREREMYENFAELFAIIKATEKLEKAYIRD+I PS+YE+EC KLI HFKTLS
Sbjct: 2   MEVKLWNDKREREMYENFAELFAIIKATEKLEKAYIRDLINPSEYESECQKLIVHFKTLS 61

Query: 61  SSLKDTVPSIESFADTYKMDCRAALNRLVTSGVPATVEHRSAAASSSTNSAATVAECVQN 120
           ++LKDTVP+IE FADTYKMDC AAL RLVTSG+PATVEHR+  A+S++NSA+ VAECVQN
Sbjct: 62  ATLKDTVPNIERFADTYKMDCPAALYRLVTSGLPATVEHRATVAASTSNSASIVAECVQN 121

Query: 121 FITSMDSLKLNMVAVDQVYPLLSDLSTSLNKLSLLPPDFEGKTKMKEWLGRLSKMGASDE 180
           FITSMDSLKLNMVAVDQVYPLLSDLS SLNKLS+LPPDFEGKTKMKEWL RLSKMGA+DE
Sbjct: 122 FITSMDSLKLNMVAVDQVYPLLSDLSASLNKLSILPPDFEGKTKMKEWLSRLSKMGAADE 181

Query: 181 LTEQQARQLHFDLESSYNSFMAALPHAGN 210
           LTEQQ+RQLHFDLESSYNSFMAALP AGN
Sbjct: 182 LTEQQSRQLHFDLESSYNSFMAALPKAGN 210

[Close]

BLAST of Spo09366.1 vs. TAIR (Arabidopsis)
Match: AT4G21560.1 (vacuolar protein sorting-associated protein 28 homolog 1)

HSP 1 Score: 355.1 bits (910), Expect = 2.900e-98
Identity = 182/209 (87.08%), Postives = 199/209 (95.22%), Query Frame = 1


		  
Query: 1   MEVKLWNDKREREMYENFAELFAIIKATEKLEKAYIRDVIAPSDYETECLKLIAHFKTLS 60
           MEVKLWNDKREREMYENFAEL+AIIKATEKLEKAYIRD+I+PS+YETEC KLI HFKTLS
Sbjct: 1   MEVKLWNDKREREMYENFAELYAIIKATEKLEKAYIRDLISPSEYETECQKLIVHFKTLS 60

Query: 61  SSLKDTVPSIESFADTYKMDCRAALNRLVTSGVPATVEHRSAAASSSTNSAATVAECVQN 120
           +SLKD VP+IE FA+TYKMDC AA+ RLVTSGVPATVEHR+AA++S+++SA+ VAECVQN
Sbjct: 61  ASLKDMVPNIERFAETYKMDCSAAVYRLVTSGVPATVEHRAAASASTSSSASVVAECVQN 120

Query: 121 FITSMDSLKLNMVAVDQVYPLLSDLSTSLNKLSLLPPDFEGKTKMKEWLGRLSKMGASDE 180
           FITSMDSLKLNMVAVDQVYPLLSDLS SLNKLS+LPPDFEGK KMKEWL RLSKMGASDE
Sbjct: 121 FITSMDSLKLNMVAVDQVYPLLSDLSASLNKLSILPPDFEGKIKMKEWLLRLSKMGASDE 180

Query: 181 LTEQQARQLHFDLESSYNSFMAALPHAGN 210
           LTEQQARQLHFDLESSYNSFMAALP+AGN
Sbjct: 181 LTEQQARQLHFDLESSYNSFMAALPNAGN 209

The following BLAST results are available for this feature:

BLAST of Spo09366.1 vs. NCBI nr
BLAST of Spo09366.1 vs. UniProtKB/TrEMBL
BLAST of Spo09366.1 vs. ExPASy Swiss-Prot
BLAST of Spo09366.1 vs. TAIR (Arabidopsis)

BLAST of Spo09366.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902184823\|gb\|KNA10377.1\|	9.9e-109	100.	hypothetical protein SOVF_1448...	[more]
gi\|731369614\|ref\|XP_010696614.1\|	4.0e-102	93.3	PREDICTED: vacuolar protein so...	[more]
gi\|731354719\|ref\|XP_010688749.1\|	2.6e-101	92.3	PREDICTED: vacuolar protein so...	[more]
gi\|590625041\|ref\|XP_007025773.1\|	1.9e-96	87.5	Vacuolar protein sorting-assoc...	[more]
gi\|659078771\|ref\|XP_008439898.1\|	9.6e-96	87.5	PREDICTED: vacuolar protein so...	[more]

BLAST of Spo09366.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9QSV0_SPIOL	6.9e-109	100.	Uncharacterized protein OS=Spi...	[more]
A0A0J8B717_BETVU	2.8e-102	93.3	Uncharacterized protein OS=Bet...	[more]
A0A0J8BMT2_BETVU	1.8e-101	92.3	Uncharacterized protein OS=Bet...	[more]
A0A061GEU0_THECC	1.3e-96	87.5	Vacuolar protein sorting-assoc...	[more]
B9S615_RICCO	8.7e-96	88.4	Vacuolar protein sorting-assoc...	[more]

BLAST of Spo09366.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
VP282_ARATH	2.3e-97	87.0	Vacuolar protein sorting-assoc...	[more]
VP281_ARATH	5.1e-97	87.0	Vacuolar protein sorting-assoc...	[more]
VPS28_DICDI	7.0e-54	54.6	Vacuolar protein sorting-assoc...	[more]
VPS28_DROME	5.4e-38	43.5	Vacuolar protein sorting-assoc...	[more]
VPS28_HUMAN	2.9e-36	41.0	Vacuolar protein sorting-assoc...	[more]

BLAST of Spo09366.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 2


Match Name	E-value	Identity	Description
AT4G05000.1	1.3e-98	87.0	Vacuolar protein sorting-assoc...	[more]
AT4G21560.1	2.9e-98	87.0	vacuolar protein sorting-assoc...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR007143	Vacuolar protein sorting-associated, VPS28	PIR	PIRSF017535	ESCRT1_Vps28	coord: 1..205 score: 1.0
IPR007143	Vacuolar protein sorting-associated, VPS28	PANTHER	PTHR12937	VACUOLAR PROTEIN SORTING 28, ISOFORM 2 VPS28	coord: 1..209 score: 1.3E
IPR007143	Vacuolar protein sorting-associated, VPS28	PFAM	PF03997	VPS28	coord: 18..204 score: 1.6
IPR017898	Vacuolar protein sorting-associated, VPS28, N-terminal	PROFILE	PS51313	VPS28_N	coord: 1..99 score: 30
IPR017899	Vacuolar protein sorting-associated, VPS28, C-terminal	PROFILE	PS51310	VPS28_C	coord: 109..205 score: 44
None	No IPR available	unknown	SSF140111	Endosomal sorting complex assembly domain	coord: 2..97 score: 1.47
None	No IPR available	unknown	SSF140427	VPS28 C-terminal domain-like	coord: 110..204 score: 2.48

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043328	protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway
cellular_component	GO:0000813	ESCRT I complex
cellular_component	GO:0005634	nucleus
molecular_function	GO:0044877	protein-containing complex binding
molecular_function	GO:0005215	transporter activity