Spo10268.1 (mRNA) | SpinachBase

Overview


Name	Spo10268.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	(L-ascorbate oxidase) (Precursor)
Location	SpoScf_04087 : 3476 .. 6393 (-)
Sequence length	2154

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AATTTTATTCCTATTTACATACGGATTATATCTCTCTATGACTATATATATATCTAGTCCGTCCTTTAAAAACATCATTCAGTTCAATAAACAACCACTGCACGTCTGCACCCTTTTCCCTCTCCTCCTAGACTCCTAGTGTTGCCGCCTTGCCGGCCACCACGCACCACCGTACCAGTTTGTCGACGACGATGGCGTCCTATTATTATGTTGTTGTTATGATAATATTTAGTAGTTTATTAATGTCATGTATTACTGTCGAGGCTGCTGAAACCCGGTATTACAAGTGGGATGTTAAATATGAACTAAAATCTCCAGATTGTTACAGGAAGTTAGTGATTACTATTAATGGCATGTTTCCTGGACCCACTATTACTGCTGAAGAAGGTGACACTATTGTTGTTCAAGTTACCAACAGCTTGTTAACTGAGAACACTGCTATCCATTGGCATGGTATTCGTCAGGTGAGTTTTATTTTATTTTATTGCCTCGATCACTCACTTTTTAGTCGATATTATATATTTTAATGTGTTTTGATTTATCGAATTTGTGTTTTACAGATTGGATCACCATGGTCTGACGGAACCGAAGGCGTTACACAATGTCCTATTGTACCCGGAGACACTTTTGAATACAAATTTGTTGTTGACAGGGTAAGATTGAATTTCACTATTTTTTTGTTTTGTTTGTTAAGATATTACACAATATAAAAGATATGCTCGAAATATTATAAGATATTTTTTTATATCTGTTAAGATATTACAAGATATCAAAAGATATGCTCTTTATATTATAACATACTCCGTAATGTTTAGATCCAATGTAAGAAAGTGTAATACGGAGTATTCGATAACGTTAAATGATTGTTTATTAGTTTTTATACAAAGTATTAATGGAACACATGTGCACATAAATGAACAGGCTGGAACATACTTGTACCATGCCCACTACGGGATGCAGAGAGAAGAAGGATTGAATGGGATGATTCAAGTTAAGCCTGCTCGTGGACAGCGAGAGCCCTTTGTGTATGACTTTGATCGTAGTATCATGCTTACAGATTGGTACCACAAGACTGCTCTTGAACATGCCATTGGCTTGAGTTCTGTTCCCTTTGTCTGGGTCGGTGAACCTCAGGTAATTAATATTTAAAAGGTCTTGTGCACATAAATAAAGTGTATATAAATATTGTTTTAATTGATGAATATTTTAAAGAGTTACATAGTTATTTTGACGGGATAATTTTATTAAAACGATTTTTTTGTCACGTACTGGGTAATTTTAAATGTTTTGACTTAGCTTTTACTCCGATTTATAATAAATTATACCGCATTTCAATAAGAATTTGTGATTACTAATTAGCATTCCAACCATGTTAATACTTGTTCTTAAGTTTGCATATTCAATTGACTAATTCAATCGCTTACCTGTTTGATCCAGTCACTCCTAATCCAAGGAAGAGGTCGGTACAACTGCTCGTCTGTCAGCATTGCCTCTGACCCTGATGTCTGTAACGCTACCCGATCCGATTGCTCGCCGTTTGTCTTGACTGTTGTTCCTGGGAAGATTTATCGCCTAAGGATTGCTAGCTTAACTTCATTATCTGCACTCAACTTTGAGATTGAGGTAACAACTAATACTACATAATTACGTATTCTACTTACTGTTTAATTGTTTATGTTAGTATGTTAATGTTACTGATTGTATATATATTGTTAATCAGGGGCATAACATGACGGTGGTTGAGACAGATGGGCATTTCGTTGAGCCATTTGAAGTCAAGAACTTGTTCATCTACTCAGGAGAGACGTACTCTGTCCTTGTGACGGCCAACCAAGACCCTTCAAGGAACTATTGGGCCACAACTAATGTTGTCAGCCGAAGGCCCGGAACTCCAACGGGTCGAGCCATCTTCAACTACTACCCAAATCACCCGGAGAAGAACCCACCAACTGTCCCTACCACAGGCCCGATGTGGAACGACTCCACATCAAGGGTAGCCCAAAGCCTAGCCATTAAGGCGCGGGAGGGGTTCATTGAAACCCCACCTGCGACCTCTGACAGAGTAATAATGCTCCTCAACACTCAAAATAGAGTTAATGGGTTCACGAAATGGGCCGTTAATAATGTATCCTTCACCTTACCCACCACCCCATACCTAGTGGCTCTCAAGGAAAACAGGCTGAGGAACAAACTCCACCACGTGTTCGACCAAAGCCCGCCTCCTACGGGCTATGATTGGCTAAACTATGACATCCATGTCAACCAATCTAATGTCAATGCCACCACAAGTAACGGCATCTATCGTCTTAAGTTTAACACTACTATTGACATTGTGTTACAAAATGCAAACATGATCAACCCGAACAACAGTGAGACCCACCCGTGGCATTTGCATGGGCATGACTTTTGGGTGTTGGGACATGGAGTGGGAAAGTTTGACCCGTTCAATGACCCGAAGAAATACAACTTGGTTAACCCGATATTGAAGAATACGGTTCCTCTTCATCCATACGGGTGGACTGCTATAAGATTCCAAGCTAATAATCCGGGTGTTTGGTTGTTCCATTGTCATATTGATTCTCATTTCGCAATGGGTATGGGAATTGTTTTTGAAGAAGGAATTGAAAGAGTTGGAAAATTGCCTACCTCCATTATGGGCTGTGGACTCACCAAACGCCTCATTACTCCTTAGTTCTTCCTTGGGCTAATTTAGCACTCTTTACTCCCAATACCAAAAACATTCTTTTATTTTTCATACCAAGTGTTTGTGTATATACATCAATACATGTATAGGGAGTGTAAGTTATTCGGATAAAGACCCAAGTATAGTCGAATGCGAAACATTTTGTATTAAATCTGGATTCAGAAAATTTTAATGAAGACTTCCTTGATAATAAAAGTTCGGAAATTTGATTTGA

mRNA sequence

AATTTTATTCCTATTTACATACGGATTATATCTCTCTATGACTATATATATATCTAGTCCGTCCTTTAAAAACATCATTCAGTTCAATAAACAACCACTGCACGTCTGCACCCTTTTCCCTCTCCTCCTAGACTCCTAGTGTTGCCGCCTTGCCGGCCACCACGCACCACCGTACCAGTTTGTCGACGACGATGGCGTCCTATTATTATGTTGTTGTTATGATAATATTTAGTAGTTTATTAATGTCATGTATTACTGTCGAGGCTGCTGAAACCCGGTATTACAAGTGGGATGTTAAATATGAACTAAAATCTCCAGATTGTTACAGGAAGTTAGTGATTACTATTAATGGCATGTTTCCTGGACCCACTATTACTGCTGAAGAAGGTGACACTATTGTTGTTCAAGTTACCAACAGCTTGTTAACTGAGAACACTGCTATCCATTGGCATGGTATTCGTCAGATTGGATCACCATGGTCTGACGGAACCGAAGGCGTTACACAATGTCCTATTGTACCCGGAGACACTTTTGAATACAAATTTGTTGTTGACAGGGCTGGAACATACTTGTACCATGCCCACTACGGGATGCAGAGAGAAGAAGGATTGAATGGGATGATTCAAGTTAAGCCTGCTCGTGGACAGCGAGAGCCCTTTGTGTATGACTTTGATCGTAGTATCATGCTTACAGATTGGTACCACAAGACTGCTCTTGAACATGCCATTGGCTTGAGTTCTGTTCCCTTTGTCTGGGTCGGTGAACCTCAGTCACTCCTAATCCAAGGAAGAGGTCGGTACAACTGCTCGTCTGTCAGCATTGCCTCTGACCCTGATGTCTGTAACGCTACCCGATCCGATTGCTCGCCGTTTGTCTTGACTGTTGTTCCTGGGAAGATTTATCGCCTAAGGATTGCTAGCTTAACTTCATTATCTGCACTCAACTTTGAGATTGAGGGGCATAACATGACGGTGGTTGAGACAGATGGGCATTTCGTTGAGCCATTTGAAGTCAAGAACTTGTTCATCTACTCAGGAGAGACGTACTCTGTCCTTGTGACGGCCAACCAAGACCCTTCAAGGAACTATTGGGCCACAACTAATGTTGTCAGCCGAAGGCCCGGAACTCCAACGGGTCGAGCCATCTTCAACTACTACCCAAATCACCCGGAGAAGAACCCACCAACTGTCCCTACCACAGGCCCGATGTGGAACGACTCCACATCAAGGGTAGCCCAAAGCCTAGCCATTAAGGCGCGGGAGGGGTTCATTGAAACCCCACCTGCGACCTCTGACAGAGTAATAATGCTCCTCAACACTCAAAATAGAGTTAATGGGTTCACGAAATGGGCCGTTAATAATGTATCCTTCACCTTACCCACCACCCCATACCTAGTGGCTCTCAAGGAAAACAGGCTGAGGAACAAACTCCACCACGTGTTCGACCAAAGCCCGCCTCCTACGGGCTATGATTGGCTAAACTATGACATCCATGTCAACCAATCTAATGTCAATGCCACCACAAGTAACGGCATCTATCGTCTTAAGTTTAACACTACTATTGACATTGTGTTACAAAATGCAAACATGATCAACCCGAACAACAGTGAGACCCACCCGTGGCATTTGCATGGGCATGACTTTTGGGTGTTGGGACATGGAGTGGGAAAGTTTGACCCGTTCAATGACCCGAAGAAATACAACTTGGTTAACCCGATATTGAAGAATACGGTTCCTCTTCATCCATACGGGTGGACTGCTATAAGATTCCAAGCTAATAATCCGGGTGTTTGGTTGTTCCATTGTCATATTGATTCTCATTTCGCAATGGGTATGGGAATTGTTTTTGAAGAAGGAATTGAAAGAGTTGGAAAATTGCCTACCTCCATTATGGGCTGTGGACTCACCAAACGCCTCATTACTCCTTAGTTCTTCCTTGGGCTAATTTAGCACTCTTTACTCCCAATACCAAAAACATTCTTTTATTTTTCATACCAAGTGTTTGTGTATATACATCAATACATGTATAGGGAGTGTAAGTTATTCGGATAAAGACCCAAGTATAGTCGAATGCGAAACATTTTGTATTAAATCTGGATTCAGAAAATTTTAATGAAGACTTCCTTGATAATAAAAGTTCGGAAATTTGATTTGA

Coding sequence (CDS)

ATGGCGTCCTATTATTATGTTGTTGTTATGATAATATTTAGTAGTTTATTAATGTCATGTATTACTGTCGAGGCTGCTGAAACCCGGTATTACAAGTGGGATGTTAAATATGAACTAAAATCTCCAGATTGTTACAGGAAGTTAGTGATTACTATTAATGGCATGTTTCCTGGACCCACTATTACTGCTGAAGAAGGTGACACTATTGTTGTTCAAGTTACCAACAGCTTGTTAACTGAGAACACTGCTATCCATTGGCATGGTATTCGTCAGATTGGATCACCATGGTCTGACGGAACCGAAGGCGTTACACAATGTCCTATTGTACCCGGAGACACTTTTGAATACAAATTTGTTGTTGACAGGGCTGGAACATACTTGTACCATGCCCACTACGGGATGCAGAGAGAAGAAGGATTGAATGGGATGATTCAAGTTAAGCCTGCTCGTGGACAGCGAGAGCCCTTTGTGTATGACTTTGATCGTAGTATCATGCTTACAGATTGGTACCACAAGACTGCTCTTGAACATGCCATTGGCTTGAGTTCTGTTCCCTTTGTCTGGGTCGGTGAACCTCAGTCACTCCTAATCCAAGGAAGAGGTCGGTACAACTGCTCGTCTGTCAGCATTGCCTCTGACCCTGATGTCTGTAACGCTACCCGATCCGATTGCTCGCCGTTTGTCTTGACTGTTGTTCCTGGGAAGATTTATCGCCTAAGGATTGCTAGCTTAACTTCATTATCTGCACTCAACTTTGAGATTGAGGGGCATAACATGACGGTGGTTGAGACAGATGGGCATTTCGTTGAGCCATTTGAAGTCAAGAACTTGTTCATCTACTCAGGAGAGACGTACTCTGTCCTTGTGACGGCCAACCAAGACCCTTCAAGGAACTATTGGGCCACAACTAATGTTGTCAGCCGAAGGCCCGGAACTCCAACGGGTCGAGCCATCTTCAACTACTACCCAAATCACCCGGAGAAGAACCCACCAACTGTCCCTACCACAGGCCCGATGTGGAACGACTCCACATCAAGGGTAGCCCAAAGCCTAGCCATTAAGGCGCGGGAGGGGTTCATTGAAACCCCACCTGCGACCTCTGACAGAGTAATAATGCTCCTCAACACTCAAAATAGAGTTAATGGGTTCACGAAATGGGCCGTTAATAATGTATCCTTCACCTTACCCACCACCCCATACCTAGTGGCTCTCAAGGAAAACAGGCTGAGGAACAAACTCCACCACGTGTTCGACCAAAGCCCGCCTCCTACGGGCTATGATTGGCTAAACTATGACATCCATGTCAACCAATCTAATGTCAATGCCACCACAAGTAACGGCATCTATCGTCTTAAGTTTAACACTACTATTGACATTGTGTTACAAAATGCAAACATGATCAACCCGAACAACAGTGAGACCCACCCGTGGCATTTGCATGGGCATGACTTTTGGGTGTTGGGACATGGAGTGGGAAAGTTTGACCCGTTCAATGACCCGAAGAAATACAACTTGGTTAACCCGATATTGAAGAATACGGTTCCTCTTCATCCATACGGGTGGACTGCTATAAGATTCCAAGCTAATAATCCGGGTGTTTGGTTGTTCCATTGTCATATTGATTCTCATTTCGCAATGGGTATGGGAATTGTTTTTGAAGAAGGAATTGAAAGAGTTGGAAAATTGCCTACCTCCATTATGGGCTGTGGACTCACCAAACGCCTCATTACTCCTTAG

Protein sequence

MASYYYVVVMIIFSSLLMSCITVEAAETRYYKWDVKYELKSPDCYRKLVITINGMFPGPTITAEEGDTIVVQVTNSLLTENTAIHWHGIRQIGSPWSDGTEGVTQCPIVPGDTFEYKFVVDRAGTYLYHAHYGMQREEGLNGMIQVKPARGQREPFVYDFDRSIMLTDWYHKTALEHAIGLSSVPFVWVGEPQSLLIQGRGRYNCSSVSIASDPDVCNATRSDCSPFVLTVVPGKIYRLRIASLTSLSALNFEIEGHNMTVVETDGHFVEPFEVKNLFIYSGETYSVLVTANQDPSRNYWATTNVVSRRPGTPTGRAIFNYYPNHPEKNPPTVPTTGPMWNDSTSRVAQSLAIKAREGFIETPPATSDRVIMLLNTQNRVNGFTKWAVNNVSFTLPTTPYLVALKENRLRNKLHHVFDQSPPPTGYDWLNYDIHVNQSNVNATTSNGIYRLKFNTTIDIVLQNANMINPNNSETHPWHLHGHDFWVLGHGVGKFDPFNDPKKYNLVNPILKNTVPLHPYGWTAIRFQANNPGVWLFHCHIDSHFAMGMGIVFEEGIERVGKLPTSIMGCGLTKRLITP

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo10268	Spo10268	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo10268.1	Spo10268.1-protein	polypeptide

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo10268.1.utr5p.1	Spo10268.1.utr5p.1	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo10268.1.CDS.5	Spo10268.1.CDS.5	CDS
Spo10268.1.CDS.4	Spo10268.1.CDS.4	CDS
Spo10268.1.CDS.3	Spo10268.1.CDS.3	CDS
Spo10268.1.CDS.2	Spo10268.1.CDS.2	CDS
Spo10268.1.CDS.1	Spo10268.1.CDS.1	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo10268.1.utr3p.1	Spo10268.1.utr3p.1	three_prime_UTR

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo10268.1.exon.5	Spo10268.1.exon.5	exon
Spo10268.1.exon.4	Spo10268.1.exon.4	exon
Spo10268.1.exon.3	Spo10268.1.exon.3	exon
Spo10268.1.exon.2	Spo10268.1.exon.2	exon
Spo10268.1.exon.1	Spo10268.1.exon.1	exon

Homology

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BLAST of Spo10268.1 vs. NCBI nr
Match: gi|902035835|gb|KNA02633.1| (hypothetical protein SOVF_216880 [Spinacia oleracea])

HSP 1 Score: 1188.3 bits (3073), Expect = 0.000e+0
Identity = 561/561 (100.00%), Postives = 561/561 (100.00%), Query Frame = 1


		  
Query: 18  MSCITVEAAETRYYKWDVKYELKSPDCYRKLVITINGMFPGPTITAEEGDTIVVQVTNSL 77
           MSCITVEAAETRYYKWDVKYELKSPDCYRKLVITINGMFPGPTITAEEGDTIVVQVTNSL
Sbjct: 1   MSCITVEAAETRYYKWDVKYELKSPDCYRKLVITINGMFPGPTITAEEGDTIVVQVTNSL 60

Query: 78  LTENTAIHWHGIRQIGSPWSDGTEGVTQCPIVPGDTFEYKFVVDRAGTYLYHAHYGMQRE 137
           LTENTAIHWHGIRQIGSPWSDGTEGVTQCPIVPGDTFEYKFVVDRAGTYLYHAHYGMQRE
Sbjct: 61  LTENTAIHWHGIRQIGSPWSDGTEGVTQCPIVPGDTFEYKFVVDRAGTYLYHAHYGMQRE 120

Query: 138 EGLNGMIQVKPARGQREPFVYDFDRSIMLTDWYHKTALEHAIGLSSVPFVWVGEPQSLLI 197
           EGLNGMIQVKPARGQREPFVYDFDRSIMLTDWYHKTALEHAIGLSSVPFVWVGEPQSLLI
Sbjct: 121 EGLNGMIQVKPARGQREPFVYDFDRSIMLTDWYHKTALEHAIGLSSVPFVWVGEPQSLLI 180

Query: 198 QGRGRYNCSSVSIASDPDVCNATRSDCSPFVLTVVPGKIYRLRIASLTSLSALNFEIEGH 257
           QGRGRYNCSSVSIASDPDVCNATRSDCSPFVLTVVPGKIYRLRIASLTSLSALNFEIEGH
Sbjct: 181 QGRGRYNCSSVSIASDPDVCNATRSDCSPFVLTVVPGKIYRLRIASLTSLSALNFEIEGH 240

Query: 258 NMTVVETDGHFVEPFEVKNLFIYSGETYSVLVTANQDPSRNYWATTNVVSRRPGTPTGRA 317
           NMTVVETDGHFVEPFEVKNLFIYSGETYSVLVTANQDPSRNYWATTNVVSRRPGTPTGRA
Sbjct: 241 NMTVVETDGHFVEPFEVKNLFIYSGETYSVLVTANQDPSRNYWATTNVVSRRPGTPTGRA 300

Query: 318 IFNYYPNHPEKNPPTVPTTGPMWNDSTSRVAQSLAIKAREGFIETPPATSDRVIMLLNTQ 377
           IFNYYPNHPEKNPPTVPTTGPMWNDSTSRVAQSLAIKAREGFIETPPATSDRVIMLLNTQ
Sbjct: 301 IFNYYPNHPEKNPPTVPTTGPMWNDSTSRVAQSLAIKAREGFIETPPATSDRVIMLLNTQ 360

Query: 378 NRVNGFTKWAVNNVSFTLPTTPYLVALKENRLRNKLHHVFDQSPPPTGYDWLNYDIHVNQ 437
           NRVNGFTKWAVNNVSFTLPTTPYLVALKENRLRNKLHHVFDQSPPPTGYDWLNYDIHVNQ
Sbjct: 361 NRVNGFTKWAVNNVSFTLPTTPYLVALKENRLRNKLHHVFDQSPPPTGYDWLNYDIHVNQ 420

Query: 438 SNVNATTSNGIYRLKFNTTIDIVLQNANMINPNNSETHPWHLHGHDFWVLGHGVGKFDPF 497
           SNVNATTSNGIYRLKFNTTIDIVLQNANMINPNNSETHPWHLHGHDFWVLGHGVGKFDPF
Sbjct: 421 SNVNATTSNGIYRLKFNTTIDIVLQNANMINPNNSETHPWHLHGHDFWVLGHGVGKFDPF 480

Query: 498 NDPKKYNLVNPILKNTVPLHPYGWTAIRFQANNPGVWLFHCHIDSHFAMGMGIVFEEGIE 557
           NDPKKYNLVNPILKNTVPLHPYGWTAIRFQANNPGVWLFHCHIDSHFAMGMGIVFEEGIE
Sbjct: 481 NDPKKYNLVNPILKNTVPLHPYGWTAIRFQANNPGVWLFHCHIDSHFAMGMGIVFEEGIE 540

Query: 558 RVGKLPTSIMGCGLTKRLITP 579
           RVGKLPTSIMGCGLTKRLITP
Sbjct: 541 RVGKLPTSIMGCGLTKRLITP 561

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BLAST of Spo10268.1 vs. NCBI nr
Match: gi|731338940|ref|XP_010680584.1| (PREDICTED: L-ascorbate oxidase [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 1018.8 bits (2633), Expect = 3.600e-294
Identity = 474/575 (82.43%), Postives = 519/575 (90.26%), Query Frame = 1


		  
Query: 5   YYVVVMIIFSSLLMSCITVEAAETRYYKWDVKYELKSPDCYRKLVITINGMFPGPTITAE 64
           YYV+ MI+ S  LMSC T+EA +TRYYKWDVKYE KSPDCYRKL ITINGM PGPTITAE
Sbjct: 18  YYVIFMIVLS--LMSC-TIEA-KTRYYKWDVKYEFKSPDCYRKLAITINGMSPGPTITAE 77

Query: 65  EGDTIVVQVTNSLLTENTAIHWHGIRQIGSPWSDGTEGVTQCPIVPGDTFEYKFVVDRAG 124
           +GDT+VV+VTN LLTEN AIHWHGIRQIG+PWSDGTEGV+QCP++PGDTF YKFVVDR G
Sbjct: 78  QGDTVVVEVTNGLLTENLAIHWHGIRQIGTPWSDGTEGVSQCPVIPGDTFVYKFVVDRPG 137

Query: 125 TYLYHAHYGMQREEGLNGMIQVKPARGQREPFVYDFDRSIMLTDWYHKTALEHAIGLSSV 184
           TYLYHAHYGMQREEGLNGMI V    G+ EPF YDFDRSIMLTDWYHK+A EHA+GL+S+
Sbjct: 138 TYLYHAHYGMQREEGLNGMIHVSLPVGESEPFTYDFDRSIMLTDWYHKSAFEHAVGLASI 197

Query: 185 PFVWVGEPQSLLIQGRGRYNCSSVSI-ASDPDVCNATRSDCSPFVLTVVPGKIYRLRIAS 244
           PFVWVGEP+SLLIQGRGR+NCSS +I AS+P +CNAT  +C+PFVLTV PGK YRLRI S
Sbjct: 198 PFVWVGEPESLLIQGRGRFNCSSDTIPASEPGICNATNPECTPFVLTVSPGKTYRLRIGS 257

Query: 245 LTSLSALNFEIEGHNMTVVETDGHFVEPFEVKNLFIYSGETYSVLVTANQDPSRNYWATT 304
           LTSLSALNFEIEGHNMTVVE DGH+VEPFEV+NLFIYSGETYSVLV ANQDPSRNYWAT+
Sbjct: 258 LTSLSALNFEIEGHNMTVVEADGHYVEPFEVQNLFIYSGETYSVLVKANQDPSRNYWATS 317

Query: 305 NVVSRRPGTPTGRAIFNYYPNHPEKNPPTVPTTGPMWNDSTSRVAQSLAIKAREGFIETP 364
           NVV RRP TPTG AIFNY PN PE++PPTVPTTGP+WN++  RVAQSLAIKAREGFIETP
Sbjct: 318 NVVGRRPSTPTGLAIFNYSPNPPEQSPPTVPTTGPIWNNTAPRVAQSLAIKAREGFIETP 377

Query: 365 PATSDRVIMLLNTQNRVNGFTKWAVNNVSFTLPTTPYLVALKENRLRNKLHHVFDQSPPP 424
           PAT DRVI+LLNTQNR+NG  KWA+NNVSFTLPTTPYLV LKE +LRNKLH VFDQ PPP
Sbjct: 378 PATPDRVIILLNTQNRINGHIKWAINNVSFTLPTTPYLVTLKEKKLRNKLHRVFDQRPPP 437

Query: 425 TGYDWLNYDIHVNQSNVNATTSNGIYRLKFNTTIDIVLQNANMINPNNSETHPWHLHGHD 484
            GYDW NYDI++   N NAT+SNG+YRLKFNTTIDIVLQNANM++PN S+THPWHLHGHD
Sbjct: 438 NGYDWQNYDINIPPPNANATSSNGLYRLKFNTTIDIVLQNANMLSPNTSDTHPWHLHGHD 497

Query: 485 FWVLGHGVGKFDPFNDPKKYNLVNPILKNTVPLHPYGWTAIRFQANNPGVWLFHCHIDSH 544
           FWVLG+G GKF+PFNDPKKYNLVNPILKNTVPLHPYGWTAIRFQANNPGVWLFHCHIDSH
Sbjct: 498 FWVLGYGTGKFNPFNDPKKYNLVNPILKNTVPLHPYGWTAIRFQANNPGVWLFHCHIDSH 557

Query: 545 FAMGMGIVFEEGIERVGKLPTSIMGCGLTKRLITP 579
           F+MGMGIVFEEGIERVGKLPTSIMGCGLTK L  P
Sbjct: 558 FSMGMGIVFEEGIERVGKLPTSIMGCGLTKNLTNP 588

[Close]

BLAST of Spo10268.1 vs. NCBI nr
Match: gi|870857268|gb|KMT08828.1| (hypothetical protein BVRB_6g135520 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 1011.9 bits (2615), Expect = 4.400e-292
Identity = 467/562 (83.10%), Postives = 510/562 (90.75%), Query Frame = 1


		  
Query: 18  MSCITVEAAETRYYKWDVKYELKSPDCYRKLVITINGMFPGPTITAEEGDTIVVQVTNSL 77
           MSC T+EA +TRYYKWDVKYE KSPDCYRKL ITINGM PGPTITAE+GDT+VV+VTN L
Sbjct: 1   MSC-TIEA-KTRYYKWDVKYEFKSPDCYRKLAITINGMSPGPTITAEQGDTVVVEVTNGL 60

Query: 78  LTENTAIHWHGIRQIGSPWSDGTEGVTQCPIVPGDTFEYKFVVDRAGTYLYHAHYGMQRE 137
           LTEN AIHWHGIRQIG+PWSDGTEGV+QCP++PGDTF YKFVVDR GTYLYHAHYGMQRE
Sbjct: 61  LTENLAIHWHGIRQIGTPWSDGTEGVSQCPVIPGDTFVYKFVVDRPGTYLYHAHYGMQRE 120

Query: 138 EGLNGMIQVKPARGQREPFVYDFDRSIMLTDWYHKTALEHAIGLSSVPFVWVGEPQSLLI 197
           EGLNGMI V    G+ EPF YDFDRSIMLTDWYHK+A EHA+GL+S+PFVWVGEP+SLLI
Sbjct: 121 EGLNGMIHVSLPVGESEPFTYDFDRSIMLTDWYHKSAFEHAVGLASIPFVWVGEPESLLI 180

Query: 198 QGRGRYNCSSVSI-ASDPDVCNATRSDCSPFVLTVVPGKIYRLRIASLTSLSALNFEIEG 257
           QGRGR+NCSS +I AS+P +CNAT  +C+PFVLTV PGK YRLRI SLTSLSALNFEIEG
Sbjct: 181 QGRGRFNCSSDTIPASEPGICNATNPECTPFVLTVSPGKTYRLRIGSLTSLSALNFEIEG 240

Query: 258 HNMTVVETDGHFVEPFEVKNLFIYSGETYSVLVTANQDPSRNYWATTNVVSRRPGTPTGR 317
           HNMTVVE DGH+VEPFEV+NLFIYSGETYSVLV ANQDPSRNYWAT+NVV RRP TPTG 
Sbjct: 241 HNMTVVEADGHYVEPFEVQNLFIYSGETYSVLVKANQDPSRNYWATSNVVGRRPSTPTGL 300

Query: 318 AIFNYYPNHPEKNPPTVPTTGPMWNDSTSRVAQSLAIKAREGFIETPPATSDRVIMLLNT 377
           AIFNY PN PE++PPTVPTTGP+WN++  RVAQSLAIKAREGFIETPPAT DRVI+LLNT
Sbjct: 301 AIFNYSPNPPEQSPPTVPTTGPIWNNTAPRVAQSLAIKAREGFIETPPATPDRVIILLNT 360

Query: 378 QNRVNGFTKWAVNNVSFTLPTTPYLVALKENRLRNKLHHVFDQSPPPTGYDWLNYDIHVN 437
           QNR+NG  KWA+NNVSFTLPTTPYLV LKE +LRNKLH VFDQ PPP GYDW NYDI++ 
Sbjct: 361 QNRINGHIKWAINNVSFTLPTTPYLVTLKEKKLRNKLHRVFDQRPPPNGYDWQNYDINIP 420

Query: 438 QSNVNATTSNGIYRLKFNTTIDIVLQNANMINPNNSETHPWHLHGHDFWVLGHGVGKFDP 497
             N NAT+SNG+YRLKFNTTIDIVLQNANM++PN S+THPWHLHGHDFWVLG+G GKF+P
Sbjct: 421 PPNANATSSNGLYRLKFNTTIDIVLQNANMLSPNTSDTHPWHLHGHDFWVLGYGTGKFNP 480

Query: 498 FNDPKKYNLVNPILKNTVPLHPYGWTAIRFQANNPGVWLFHCHIDSHFAMGMGIVFEEGI 557
           FNDPKKYNLVNPILKNTVPLHPYGWTAIRFQANNPGVWLFHCHIDSHF+MGMGIVFEEGI
Sbjct: 481 FNDPKKYNLVNPILKNTVPLHPYGWTAIRFQANNPGVWLFHCHIDSHFSMGMGIVFEEGI 540

Query: 558 ERVGKLPTSIMGCGLTKRLITP 579
           ERVGKLPTSIMGCGLTK L  P
Sbjct: 541 ERVGKLPTSIMGCGLTKNLTNP 560

[Close]

BLAST of Spo10268.1 vs. NCBI nr
Match: gi|743923090|ref|XP_011005626.1| (PREDICTED: L-ascorbate oxidase-like [Populus euphratica])

HSP 1 Score: 884.4 bits (2284), Expect = 1.100e-253
Identity = 401/558 (71.86%), Postives = 469/558 (84.05%), Query Frame = 1


		  
Query: 21  ITVEAAETRYYKWDVKYELKSPDCYRKLVITINGMFPGPTITAEEGDTIVVQVTNSLLTE 80
           I +  A  R+YKW++KYE KSPDCY+KLVITING  PGPTI A++ DT++V+V NSLLTE
Sbjct: 30  IPIAEARIRHYKWELKYEYKSPDCYKKLVITINGRTPGPTILAKQNDTVIVEVKNSLLTE 89

Query: 81  NTAIHWHGIRQIGSPWSDGTEGVTQCPIVPGDTFEYKFVVDRAGTYLYHAHYGMQREEGL 140
           NTAIHWHGIRQIG+PW DGTEGVTQCPI+PGDTF YKFVVDR GTYLYHAHYGMQRE G+
Sbjct: 90  NTAIHWHGIRQIGTPWFDGTEGVTQCPILPGDTFVYKFVVDRPGTYLYHAHYGMQREAGI 149

Query: 141 NGMIQVKPARGQREPFVYDFDRSIMLTDWYHKTALEHAIGLSSVPFVWVGEPQSLLIQGR 200
            G I+V    G+ EPF YD+DRSI+LTDWYHK+  EHA GLSS+PF WVGEPQSLLIQG+
Sbjct: 150 YGSIRVALPDGESEPFAYDYDRSIILTDWYHKSTYEHAAGLSSIPFQWVGEPQSLLIQGK 209

Query: 201 GRYNCSSVSIASDPDVCNATRSDCSPFVLTVVPGKIYRLRIASLTSLSALNFEIEGHNMT 260
           GR++C++ +     DVCN T  +CS +  TVVPGK YRLRI+SL++LSAL+F+IEGH+MT
Sbjct: 210 GRFDCAAANPPLKADVCNNTNPECSLYSTTVVPGKTYRLRISSLSALSALSFQIEGHSMT 269

Query: 261 VVETDGHFVEPFEVKNLFIYSGETYSVLVTANQDPSRNYWATTNVVSRRPGTPTGRAIFN 320
           VVE DGH+VEPF VKNLFIYSGETYSVLV  +QDPSRNYWA TNVVSR   TP G AIFN
Sbjct: 270 VVEADGHYVEPFVVKNLFIYSGETYSVLVKTDQDPSRNYWAATNVVSRNATTPPGLAIFN 329

Query: 321 YYPNHPEKNPPTVPTTGPMWNDSTSRVAQSLAIKAREGFIETPPATSDRVIMLLNTQNRV 380
           YYPNHP ++PPT+P +GPMWND   R  QS+AIKAR+G I  PPATSDRVI+LLNTQNRV
Sbjct: 330 YYPNHPRRSPPTIPPSGPMWNDIEPRFNQSVAIKARKGHIYYPPATSDRVIVLLNTQNRV 389

Query: 381 NGFTKWAVNNVSFTLPTTPYLVALKENRLRNKLHHVFDQSPPPTGYDWLNYDIHVNQSNV 440
           NG  +W+VNNVSF +P TPYL+ALKEN L     H F Q+PPP GYD+ NYD+   Q+N 
Sbjct: 390 NGNVRWSVNNVSFNIPHTPYLIALKENLL-----HTFSQTPPPEGYDFKNYDVFARQNNT 449

Query: 441 NATTSNGIYRLKFNTTIDIVLQNANMINPNNSETHPWHLHGHDFWVLGHGVGKFDPFNDP 500
           NATTS+ IYRL+ N+T+DI+LQNAN +NPNNSETHPWHLHGHDFWVLG+G GKFDP NDP
Sbjct: 450 NATTSDAIYRLQLNSTVDIILQNANTMNPNNSETHPWHLHGHDFWVLGYGKGKFDPINDP 509

Query: 501 KKYNLVNPILKNTVPLHPYGWTAIRFQANNPGVWLFHCHIDSHFAMGMGIVFEEGIERVG 560
           K YNLV+PI+KNTVP+HP+GWTA+RF+A+NPGVW FHCHI+SHF MGMG+VFEEGIERVG
Sbjct: 510 KNYNLVDPIMKNTVPVHPFGWTALRFKADNPGVWAFHCHIESHFFMGMGVVFEEGIERVG 569

Query: 561 KLPTSIMGCGLTKRLITP 579
           KLP+SIMGCG TK L+ P
Sbjct: 570 KLPSSIMGCGETKHLLKP 582

[Close]

BLAST of Spo10268.1 vs. NCBI nr
Match: gi|566179811|ref|XP_006380435.1| (L-ascorbate oxidase family protein [Populus trichocarpa])

HSP 1 Score: 884.0 bits (2283), Expect = 1.400e-253
Identity = 403/558 (72.22%), Postives = 467/558 (83.69%), Query Frame = 1


		  
Query: 21  ITVEAAETRYYKWDVKYELKSPDCYRKLVITINGMFPGPTITAEEGDTIVVQVTNSLLTE 80
           I +  A  R+YKW++KYE KSPDCY+KLVITING  PGPTI A++ DT++V+V NSLLTE
Sbjct: 16  IPIADARIRHYKWELKYEYKSPDCYKKLVITINGRTPGPTILAKQNDTVIVEVKNSLLTE 75

Query: 81  NTAIHWHGIRQIGSPWSDGTEGVTQCPIVPGDTFEYKFVVDRAGTYLYHAHYGMQREEGL 140
           NTAIHWHGIRQIG+PW DGTEGVTQCPI+PGDTF YKFVVDR GTYLYHAHYGMQRE G+
Sbjct: 76  NTAIHWHGIRQIGTPWFDGTEGVTQCPILPGDTFVYKFVVDRPGTYLYHAHYGMQREAGI 135

Query: 141 NGMIQVKPARGQREPFVYDFDRSIMLTDWYHKTALEHAIGLSSVPFVWVGEPQSLLIQGR 200
            G I+V    G+ EPF YD+DRSI+LTDWYHK+  E A GLSS+PF WVGEPQSLLIQG+
Sbjct: 136 YGSIRVALPDGESEPFAYDYDRSIILTDWYHKSTYEQAAGLSSIPFQWVGEPQSLLIQGK 195

Query: 201 GRYNCSSVSIASDPDVCNATRSDCSPFVLTVVPGKIYRLRIASLTSLSALNFEIEGHNMT 260
           GR++CS+ +     DVCN T  +CS +  TVVPGK YRLRI+SL++LSAL+F+IEGHNMT
Sbjct: 196 GRFDCSAANPPLKADVCNNTNPECSLYSTTVVPGKTYRLRISSLSALSALSFQIEGHNMT 255

Query: 261 VVETDGHFVEPFEVKNLFIYSGETYSVLVTANQDPSRNYWATTNVVSRRPGTPTGRAIFN 320
           VVE DGH+VEPF VKNLFIYSGETYSVLV  +QDPSRNYWATTNVVSR   TP G AIFN
Sbjct: 256 VVEADGHYVEPFVVKNLFIYSGETYSVLVKTDQDPSRNYWATTNVVSRNATTPPGLAIFN 315

Query: 321 YYPNHPEKNPPTVPTTGPMWNDSTSRVAQSLAIKAREGFIETPPATSDRVIMLLNTQNRV 380
           YYPNHP ++PPT+P +GPMWND   R  QS+AIKAR G I  PPATSDRVI+LLNTQN V
Sbjct: 316 YYPNHPRRSPPTIPPSGPMWNDIAPRFNQSVAIKARRGHIYPPPATSDRVIVLLNTQNTV 375

Query: 381 NGFTKWAVNNVSFTLPTTPYLVALKENRLRNKLHHVFDQSPPPTGYDWLNYDIHVNQSNV 440
           NG  +W+VN VSF +P TPYL+ALKEN L     H F Q+PPP GYD+ NYDI   Q+N 
Sbjct: 376 NGNVRWSVNKVSFNIPHTPYLIALKENLL-----HTFSQTPPPEGYDFKNYDIFARQNNT 435

Query: 441 NATTSNGIYRLKFNTTIDIVLQNANMINPNNSETHPWHLHGHDFWVLGHGVGKFDPFNDP 500
           NATTS+ IYRL+ N+T+DI+LQNAN +NPNNSETHPWHLHGHDFWVLG+G GKFDP NDP
Sbjct: 436 NATTSDAIYRLQLNSTVDIILQNANTMNPNNSETHPWHLHGHDFWVLGYGKGKFDPINDP 495

Query: 501 KKYNLVNPILKNTVPLHPYGWTAIRFQANNPGVWLFHCHIDSHFAMGMGIVFEEGIERVG 560
           K YNLV+PI+KNTVP+HP+GWTA+RF+A+NPGVW FHCHI+SHF MGMG+VFEEGIERVG
Sbjct: 496 KNYNLVDPIMKNTVPVHPFGWTALRFKADNPGVWAFHCHIESHFFMGMGVVFEEGIERVG 555

Query: 561 KLPTSIMGCGLTKRLITP 579
           KLP+SIMGCG TKRL+ P
Sbjct: 556 KLPSSIMGCGETKRLLKP 568

[Close]

BLAST of Spo10268.1 vs. UniProtKB/TrEMBL
Match: A0A0K9Q7I6_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_216880 PE=3 SV=1)

HSP 1 Score: 1188.3 bits (3073), Expect = 0.000e+0
Identity = 561/561 (100.00%), Postives = 561/561 (100.00%), Query Frame = 1


		  
Query: 18  MSCITVEAAETRYYKWDVKYELKSPDCYRKLVITINGMFPGPTITAEEGDTIVVQVTNSL 77
           MSCITVEAAETRYYKWDVKYELKSPDCYRKLVITINGMFPGPTITAEEGDTIVVQVTNSL
Sbjct: 1   MSCITVEAAETRYYKWDVKYELKSPDCYRKLVITINGMFPGPTITAEEGDTIVVQVTNSL 60

Query: 78  LTENTAIHWHGIRQIGSPWSDGTEGVTQCPIVPGDTFEYKFVVDRAGTYLYHAHYGMQRE 137
           LTENTAIHWHGIRQIGSPWSDGTEGVTQCPIVPGDTFEYKFVVDRAGTYLYHAHYGMQRE
Sbjct: 61  LTENTAIHWHGIRQIGSPWSDGTEGVTQCPIVPGDTFEYKFVVDRAGTYLYHAHYGMQRE 120

Query: 138 EGLNGMIQVKPARGQREPFVYDFDRSIMLTDWYHKTALEHAIGLSSVPFVWVGEPQSLLI 197
           EGLNGMIQVKPARGQREPFVYDFDRSIMLTDWYHKTALEHAIGLSSVPFVWVGEPQSLLI
Sbjct: 121 EGLNGMIQVKPARGQREPFVYDFDRSIMLTDWYHKTALEHAIGLSSVPFVWVGEPQSLLI 180

Query: 198 QGRGRYNCSSVSIASDPDVCNATRSDCSPFVLTVVPGKIYRLRIASLTSLSALNFEIEGH 257
           QGRGRYNCSSVSIASDPDVCNATRSDCSPFVLTVVPGKIYRLRIASLTSLSALNFEIEGH
Sbjct: 181 QGRGRYNCSSVSIASDPDVCNATRSDCSPFVLTVVPGKIYRLRIASLTSLSALNFEIEGH 240

Query: 258 NMTVVETDGHFVEPFEVKNLFIYSGETYSVLVTANQDPSRNYWATTNVVSRRPGTPTGRA 317
           NMTVVETDGHFVEPFEVKNLFIYSGETYSVLVTANQDPSRNYWATTNVVSRRPGTPTGRA
Sbjct: 241 NMTVVETDGHFVEPFEVKNLFIYSGETYSVLVTANQDPSRNYWATTNVVSRRPGTPTGRA 300

Query: 318 IFNYYPNHPEKNPPTVPTTGPMWNDSTSRVAQSLAIKAREGFIETPPATSDRVIMLLNTQ 377
           IFNYYPNHPEKNPPTVPTTGPMWNDSTSRVAQSLAIKAREGFIETPPATSDRVIMLLNTQ
Sbjct: 301 IFNYYPNHPEKNPPTVPTTGPMWNDSTSRVAQSLAIKAREGFIETPPATSDRVIMLLNTQ 360

Query: 378 NRVNGFTKWAVNNVSFTLPTTPYLVALKENRLRNKLHHVFDQSPPPTGYDWLNYDIHVNQ 437
           NRVNGFTKWAVNNVSFTLPTTPYLVALKENRLRNKLHHVFDQSPPPTGYDWLNYDIHVNQ
Sbjct: 361 NRVNGFTKWAVNNVSFTLPTTPYLVALKENRLRNKLHHVFDQSPPPTGYDWLNYDIHVNQ 420

Query: 438 SNVNATTSNGIYRLKFNTTIDIVLQNANMINPNNSETHPWHLHGHDFWVLGHGVGKFDPF 497
           SNVNATTSNGIYRLKFNTTIDIVLQNANMINPNNSETHPWHLHGHDFWVLGHGVGKFDPF
Sbjct: 421 SNVNATTSNGIYRLKFNTTIDIVLQNANMINPNNSETHPWHLHGHDFWVLGHGVGKFDPF 480

Query: 498 NDPKKYNLVNPILKNTVPLHPYGWTAIRFQANNPGVWLFHCHIDSHFAMGMGIVFEEGIE 557
           NDPKKYNLVNPILKNTVPLHPYGWTAIRFQANNPGVWLFHCHIDSHFAMGMGIVFEEGIE
Sbjct: 481 NDPKKYNLVNPILKNTVPLHPYGWTAIRFQANNPGVWLFHCHIDSHFAMGMGIVFEEGIE 540

Query: 558 RVGKLPTSIMGCGLTKRLITP 579
           RVGKLPTSIMGCGLTKRLITP
Sbjct: 541 RVGKLPTSIMGCGLTKRLITP 561

[Close]

BLAST of Spo10268.1 vs. UniProtKB/TrEMBL
Match: A0A0J8C9Y3_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_6g135520 PE=3 SV=1)

HSP 1 Score: 1011.9 bits (2615), Expect = 3.100e-292
Identity = 467/562 (83.10%), Postives = 510/562 (90.75%), Query Frame = 1


		  
Query: 18  MSCITVEAAETRYYKWDVKYELKSPDCYRKLVITINGMFPGPTITAEEGDTIVVQVTNSL 77
           MSC T+EA +TRYYKWDVKYE KSPDCYRKL ITINGM PGPTITAE+GDT+VV+VTN L
Sbjct: 1   MSC-TIEA-KTRYYKWDVKYEFKSPDCYRKLAITINGMSPGPTITAEQGDTVVVEVTNGL 60

Query: 78  LTENTAIHWHGIRQIGSPWSDGTEGVTQCPIVPGDTFEYKFVVDRAGTYLYHAHYGMQRE 137
           LTEN AIHWHGIRQIG+PWSDGTEGV+QCP++PGDTF YKFVVDR GTYLYHAHYGMQRE
Sbjct: 61  LTENLAIHWHGIRQIGTPWSDGTEGVSQCPVIPGDTFVYKFVVDRPGTYLYHAHYGMQRE 120

Query: 138 EGLNGMIQVKPARGQREPFVYDFDRSIMLTDWYHKTALEHAIGLSSVPFVWVGEPQSLLI 197
           EGLNGMI V    G+ EPF YDFDRSIMLTDWYHK+A EHA+GL+S+PFVWVGEP+SLLI
Sbjct: 121 EGLNGMIHVSLPVGESEPFTYDFDRSIMLTDWYHKSAFEHAVGLASIPFVWVGEPESLLI 180

Query: 198 QGRGRYNCSSVSI-ASDPDVCNATRSDCSPFVLTVVPGKIYRLRIASLTSLSALNFEIEG 257
           QGRGR+NCSS +I AS+P +CNAT  +C+PFVLTV PGK YRLRI SLTSLSALNFEIEG
Sbjct: 181 QGRGRFNCSSDTIPASEPGICNATNPECTPFVLTVSPGKTYRLRIGSLTSLSALNFEIEG 240

Query: 258 HNMTVVETDGHFVEPFEVKNLFIYSGETYSVLVTANQDPSRNYWATTNVVSRRPGTPTGR 317
           HNMTVVE DGH+VEPFEV+NLFIYSGETYSVLV ANQDPSRNYWAT+NVV RRP TPTG 
Sbjct: 241 HNMTVVEADGHYVEPFEVQNLFIYSGETYSVLVKANQDPSRNYWATSNVVGRRPSTPTGL 300

Query: 318 AIFNYYPNHPEKNPPTVPTTGPMWNDSTSRVAQSLAIKAREGFIETPPATSDRVIMLLNT 377
           AIFNY PN PE++PPTVPTTGP+WN++  RVAQSLAIKAREGFIETPPAT DRVI+LLNT
Sbjct: 301 AIFNYSPNPPEQSPPTVPTTGPIWNNTAPRVAQSLAIKAREGFIETPPATPDRVIILLNT 360

Query: 378 QNRVNGFTKWAVNNVSFTLPTTPYLVALKENRLRNKLHHVFDQSPPPTGYDWLNYDIHVN 437
           QNR+NG  KWA+NNVSFTLPTTPYLV LKE +LRNKLH VFDQ PPP GYDW NYDI++ 
Sbjct: 361 QNRINGHIKWAINNVSFTLPTTPYLVTLKEKKLRNKLHRVFDQRPPPNGYDWQNYDINIP 420

Query: 438 QSNVNATTSNGIYRLKFNTTIDIVLQNANMINPNNSETHPWHLHGHDFWVLGHGVGKFDP 497
             N NAT+SNG+YRLKFNTTIDIVLQNANM++PN S+THPWHLHGHDFWVLG+G GKF+P
Sbjct: 421 PPNANATSSNGLYRLKFNTTIDIVLQNANMLSPNTSDTHPWHLHGHDFWVLGYGTGKFNP 480

Query: 498 FNDPKKYNLVNPILKNTVPLHPYGWTAIRFQANNPGVWLFHCHIDSHFAMGMGIVFEEGI 557
           FNDPKKYNLVNPILKNTVPLHPYGWTAIRFQANNPGVWLFHCHIDSHF+MGMGIVFEEGI
Sbjct: 481 FNDPKKYNLVNPILKNTVPLHPYGWTAIRFQANNPGVWLFHCHIDSHFSMGMGIVFEEGI 540

Query: 558 ERVGKLPTSIMGCGLTKRLITP 579
           ERVGKLPTSIMGCGLTK L  P
Sbjct: 541 ERVGKLPTSIMGCGLTKNLTNP 560

[Close]

BLAST of Spo10268.1 vs. UniProtKB/TrEMBL
Match: U5G9V4_POPTR (L-ascorbate oxidase family protein OS=Populus trichocarpa GN=POPTR_0007s05890g PE=3 SV=1)

HSP 1 Score: 884.0 bits (2283), Expect = 9.700e-254
Identity = 403/558 (72.22%), Postives = 467/558 (83.69%), Query Frame = 1


		  
Query: 21  ITVEAAETRYYKWDVKYELKSPDCYRKLVITINGMFPGPTITAEEGDTIVVQVTNSLLTE 80
           I +  A  R+YKW++KYE KSPDCY+KLVITING  PGPTI A++ DT++V+V NSLLTE
Sbjct: 16  IPIADARIRHYKWELKYEYKSPDCYKKLVITINGRTPGPTILAKQNDTVIVEVKNSLLTE 75

Query: 81  NTAIHWHGIRQIGSPWSDGTEGVTQCPIVPGDTFEYKFVVDRAGTYLYHAHYGMQREEGL 140
           NTAIHWHGIRQIG+PW DGTEGVTQCPI+PGDTF YKFVVDR GTYLYHAHYGMQRE G+
Sbjct: 76  NTAIHWHGIRQIGTPWFDGTEGVTQCPILPGDTFVYKFVVDRPGTYLYHAHYGMQREAGI 135

Query: 141 NGMIQVKPARGQREPFVYDFDRSIMLTDWYHKTALEHAIGLSSVPFVWVGEPQSLLIQGR 200
            G I+V    G+ EPF YD+DRSI+LTDWYHK+  E A GLSS+PF WVGEPQSLLIQG+
Sbjct: 136 YGSIRVALPDGESEPFAYDYDRSIILTDWYHKSTYEQAAGLSSIPFQWVGEPQSLLIQGK 195

Query: 201 GRYNCSSVSIASDPDVCNATRSDCSPFVLTVVPGKIYRLRIASLTSLSALNFEIEGHNMT 260
           GR++CS+ +     DVCN T  +CS +  TVVPGK YRLRI+SL++LSAL+F+IEGHNMT
Sbjct: 196 GRFDCSAANPPLKADVCNNTNPECSLYSTTVVPGKTYRLRISSLSALSALSFQIEGHNMT 255

Query: 261 VVETDGHFVEPFEVKNLFIYSGETYSVLVTANQDPSRNYWATTNVVSRRPGTPTGRAIFN 320
           VVE DGH+VEPF VKNLFIYSGETYSVLV  +QDPSRNYWATTNVVSR   TP G AIFN
Sbjct: 256 VVEADGHYVEPFVVKNLFIYSGETYSVLVKTDQDPSRNYWATTNVVSRNATTPPGLAIFN 315

Query: 321 YYPNHPEKNPPTVPTTGPMWNDSTSRVAQSLAIKAREGFIETPPATSDRVIMLLNTQNRV 380
           YYPNHP ++PPT+P +GPMWND   R  QS+AIKAR G I  PPATSDRVI+LLNTQN V
Sbjct: 316 YYPNHPRRSPPTIPPSGPMWNDIAPRFNQSVAIKARRGHIYPPPATSDRVIVLLNTQNTV 375

Query: 381 NGFTKWAVNNVSFTLPTTPYLVALKENRLRNKLHHVFDQSPPPTGYDWLNYDIHVNQSNV 440
           NG  +W+VN VSF +P TPYL+ALKEN L     H F Q+PPP GYD+ NYDI   Q+N 
Sbjct: 376 NGNVRWSVNKVSFNIPHTPYLIALKENLL-----HTFSQTPPPEGYDFKNYDIFARQNNT 435

Query: 441 NATTSNGIYRLKFNTTIDIVLQNANMINPNNSETHPWHLHGHDFWVLGHGVGKFDPFNDP 500
           NATTS+ IYRL+ N+T+DI+LQNAN +NPNNSETHPWHLHGHDFWVLG+G GKFDP NDP
Sbjct: 436 NATTSDAIYRLQLNSTVDIILQNANTMNPNNSETHPWHLHGHDFWVLGYGKGKFDPINDP 495

Query: 501 KKYNLVNPILKNTVPLHPYGWTAIRFQANNPGVWLFHCHIDSHFAMGMGIVFEEGIERVG 560
           K YNLV+PI+KNTVP+HP+GWTA+RF+A+NPGVW FHCHI+SHF MGMG+VFEEGIERVG
Sbjct: 496 KNYNLVDPIMKNTVPVHPFGWTALRFKADNPGVWAFHCHIESHFFMGMGVVFEEGIERVG 555

Query: 561 KLPTSIMGCGLTKRLITP 579
           KLP+SIMGCG TKRL+ P
Sbjct: 556 KLPSSIMGCGETKRLLKP 568

[Close]

BLAST of Spo10268.1 vs. UniProtKB/TrEMBL
Match: M5X9Y5_PRUPE (Uncharacterized protein OS=Prunus persica GN=PRUPE_ppa003375mg PE=3 SV=1)

HSP 1 Score: 879.0 bits (2270), Expect = 3.100e-252
Identity = 399/563 (70.87%), Postives = 475/563 (84.37%), Query Frame = 1


		  
Query: 17  LMSCITVEAAETRYYKWDVKYELKSPDCYRKLVITINGMFPGPTITAEEGDTIVVQVTNS 76
           L++ +TVEA   R+Y+W+VKYE KSPDC++KL ITING  PGP I A++GDTIVV++ NS
Sbjct: 22  LVAVVTVEA-RIRHYRWEVKYEYKSPDCFKKLAITINGGTPGPKILAQQGDTIVVELKNS 81

Query: 77  LLTENTAIHWHGIRQIGSPWSDGTEGVTQCPIVPGDTFEYKFVVDRAGTYLYHAHYGMQR 136
           LLTEN AIHWHGIRQIG+PWSDGTEGVTQCPI PGDTF YKFVVDRAG+YLYHAHYG+QR
Sbjct: 82  LLTENVAIHWHGIRQIGTPWSDGTEGVTQCPIFPGDTFIYKFVVDRAGSYLYHAHYGIQR 141

Query: 137 EEGLNGMIQVKPARGQREPFVYDFDRSIMLTDWYHKTALEHAIGLSSVPFVWVGEPQSLL 196
           E GL G I V    G+ EPF YD+DRSI+L DW+HK+  E A+GLSS+PFVWVGEPQSLL
Sbjct: 142 EAGLYGSITVSVPNGESEPFAYDYDRSIILNDWFHKSTYEQAVGLSSIPFVWVGEPQSLL 201

Query: 197 IQGRGRYNCSSVSIAS-DPDVCNATRSDCSPFVLTVVPGKIYRLRIASLTSLSALNFEIE 256
           I GRGR+NCS ++  S +  VCN T  +CSP++LT++P K YRLRI SLTSLSALNF+IE
Sbjct: 202 INGRGRFNCSLLATPSLEVGVCNTTNPECSPYILTIIPDKTYRLRIGSLTSLSALNFQIE 261

Query: 257 GHNMTVVETDGHFVEPFEVKNLFIYSGETYSVLVTANQDPSRNYWATTNVVSRRPGTPTG 316
           GHNMTVVE DGH+VEPF+ KNL+IYSGETYSVLVTANQDPSRNYW TTNVVSR+P TPTG
Sbjct: 262 GHNMTVVEADGHYVEPFQTKNLYIYSGETYSVLVTANQDPSRNYWVTTNVVSRKPATPTG 321

Query: 317 RAIFNYYPNHPEKNPPTVPTTGPMWNDSTSRVAQSLAIKAREGFIETPPATSDRVIMLLN 376
            AI NYYP + +K+PPT+PTTGP+WNDS  R+AQSL+IKA + FI +P  TSD+VI+LLN
Sbjct: 322 LAILNYYPKYFKKSPPTLPTTGPLWNDSVPRLAQSLSIKAHKSFIHSPALTSDKVIVLLN 381

Query: 377 TQNRVNGFTKWAVNNVSFTLPTTPYLVALKENRLRNKLHHVFDQSPPPTGYDWLNYDIHV 436
           TQN+++G+ +W++NNVSF LP TPYL+ALKEN     L+HVFDQ+PPP  YD+ NYDI+ 
Sbjct: 382 TQNKIDGYYRWSLNNVSFYLPHTPYLIALKENL---NLNHVFDQTPPPENYDFKNYDIYA 441

Query: 437 NQSNVNATTSNGIYRLKFNTTIDIVLQNANMINPNNSETHPWHLHGHDFWVLGHGVGKFD 496
              NVNAT+SNGIYRLKFNTT+DI+LQNAN +  NNSETHPWHLHGHDFWVLG+G GKFD
Sbjct: 442 VAENVNATSSNGIYRLKFNTTVDIILQNANTMTVNNSETHPWHLHGHDFWVLGYGTGKFD 501

Query: 497 PFNDPKKYNLVNPILKNTVPLHPYGWTAIRFQANNPGVWLFHCHIDSHFAMGMGIVFEEG 556
              D  KYNLVNPI+KNTVP+HPYGWTA+RF+A+NPGVW FHCH++SHF MGMG+VFE G
Sbjct: 502 MMKDVGKYNLVNPIMKNTVPVHPYGWTALRFRADNPGVWAFHCHLESHFYMGMGVVFEAG 561

Query: 557 IERVGKLPTSIMGCGLTKRLITP 579
           +E+VGKLP+S+MGCG TK    P
Sbjct: 562 VEKVGKLPSSVMGCGETKGFHRP 580

[Close]

BLAST of Spo10268.1 vs. UniProtKB/TrEMBL
Match: B9HFP2_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0007s05910g PE=3 SV=2)

HSP 1 Score: 877.5 bits (2266), Expect = 9.100e-252
Identity = 400/558 (71.68%), Postives = 467/558 (83.69%), Query Frame = 1


		  
Query: 21  ITVEAAETRYYKWDVKYELKSPDCYRKLVITINGMFPGPTITAEEGDTIVVQVTNSLLTE 80
           I +  A  R+YK+++KYE KSPDCY+KLVITING  PGPTI A++ DT++V+V NSLLTE
Sbjct: 30  IPIAEARIRHYKFELKYEYKSPDCYKKLVITINGRTPGPTIFAQQNDTVIVEVKNSLLTE 89

Query: 81  NTAIHWHGIRQIGSPWSDGTEGVTQCPIVPGDTFEYKFVVDRAGTYLYHAHYGMQREEGL 140
           NTAIHWHGIRQIG+PW DGTEGVTQCPI+PGDTF YKFVVDR GTYLYHAHYGMQRE G+
Sbjct: 90  NTAIHWHGIRQIGTPWFDGTEGVTQCPILPGDTFVYKFVVDRPGTYLYHAHYGMQREAGI 149

Query: 141 NGMIQVKPARGQREPFVYDFDRSIMLTDWYHKTALEHAIGLSSVPFVWVGEPQSLLIQGR 200
            G I+V    G+ EPF YD+DRSI+LTDWYHK+  E A GLSS+PF WVGEPQSLLIQG+
Sbjct: 150 YGSIRVALPDGESEPFAYDYDRSIILTDWYHKSTYEQATGLSSIPFQWVGEPQSLLIQGK 209

Query: 201 GRYNCSSVSIASDPDVCNATRSDCSPFVLTVVPGKIYRLRIASLTSLSALNFEIEGHNMT 260
           GR++CS+ +     DVCN T  +CS +  TVVPGK YRLRI+SLT+LSAL+F+IEGH++T
Sbjct: 210 GRFDCSAANPPLKADVCNNTSPECSLYSTTVVPGKTYRLRISSLTALSALSFQIEGHSLT 269

Query: 261 VVETDGHFVEPFEVKNLFIYSGETYSVLVTANQDPSRNYWATTNVVSRRPGTPTGRAIFN 320
           VVE DGH+VEPF VKNLFIYSGETYSVLV  +QDPSRNYWATTNVVSR   TP G AIFN
Sbjct: 270 VVEADGHYVEPFVVKNLFIYSGETYSVLVKTDQDPSRNYWATTNVVSRNATTPPGLAIFN 329

Query: 321 YYPNHPEKNPPTVPTTGPMWNDSTSRVAQSLAIKAREGFIETPPATSDRVIMLLNTQNRV 380
           YYPNHP ++PPT+P +GPMWND   R  QS+AIKAR+G I +PPATSDRVI+ LNTQNRV
Sbjct: 330 YYPNHPRRSPPTIPPSGPMWNDIEPRFNQSVAIKARKGHIYSPPATSDRVIVFLNTQNRV 389

Query: 381 NGFTKWAVNNVSFTLPTTPYLVALKENRLRNKLHHVFDQSPPPTGYDWLNYDIHVNQSNV 440
           NG  +W+VNNVSF +P TPYL+ALKEN L     H F Q+PPP GYD+ NYDI   Q+N 
Sbjct: 390 NGNVRWSVNNVSFNIPHTPYLIALKENLL-----HTFSQTPPPEGYDFKNYDIFARQNNT 449

Query: 441 NATTSNGIYRLKFNTTIDIVLQNANMINPNNSETHPWHLHGHDFWVLGHGVGKFDPFNDP 500
           NATTS+ IYRL+ N+T+DI+LQNAN +NPNNSETHPWHLHGHDFWVLG+G GKFDP NDP
Sbjct: 450 NATTSDAIYRLQLNSTVDIILQNANTMNPNNSETHPWHLHGHDFWVLGYGKGKFDPINDP 509

Query: 501 KKYNLVNPILKNTVPLHPYGWTAIRFQANNPGVWLFHCHIDSHFAMGMGIVFEEGIERVG 560
           K YNLV+PI+KNTVP+HP GWTA+RF+A+NPG W FHCHI+SHF MGMG+VFEEGIERVG
Sbjct: 510 KNYNLVDPIMKNTVPVHPLGWTALRFKADNPGAWAFHCHIESHFFMGMGVVFEEGIERVG 569

Query: 561 KLPTSIMGCGLTKRLITP 579
           KLP+SIMGCG TK L+ P
Sbjct: 570 KLPSSIMGCGETKHLLKP 582

[Close]

BLAST of Spo10268.1 vs. ExPASy Swiss-Prot
Match: ASO_CUCSA (L-ascorbate oxidase OS=Cucumis sativus PE=1 SV=1)

HSP 1 Score: 603.6 bits (1555), Expect = 2.300e-171
Identity = 290/572 (50.70%), Postives = 379/572 (66.26%), Query Frame = 1


		  
Query: 6   YVVVMIIFSSLLMSCITVEAA---ETRYYKWDVKYELKSPDCYRKLVITINGMFPGPTIT 65
           ++V+ IIF       IT+  A   + ++YKWDV+Y   SPDC   +V+ ING FPGPTI 
Sbjct: 17  FLVLSIIFGF----GITLSEAGFPKIKHYKWDVEYMFWSPDCVENIVMGINGEFPGPTIR 76

Query: 66  AEEGDTIVVQVTNSLLTENTAIHWHGIRQIGSPWSDGTEGVTQCPIVPGDTFEYKFVVDR 125
           A  GD +VV++TN L TE   IHWHGI Q G+PW+DGT  ++QC I PG+TF Y+FVVD+
Sbjct: 77  ANAGDIVVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGETFTYRFVVDK 136

Query: 126 AGTYLYHAHYGMQREEGLNGMIQVKPARGQREPFVYDFDRSIMLTDWYHKTALEHAIGLS 185
           AGTY YH H GMQR  GL G + V P  G+ EPF YD + +++L+DW+H++  +  +GLS
Sbjct: 137 AGTYFYHGHLGMQRSAGLYGSLIVDPPEGRSEPFHYDEEINLLLSDWWHQSVHKQEVGLS 196

Query: 186 SVPFVWVGEPQSLLIQGRGRYNCS-SVSIASDPDVCNAT-RSDCSPFVLTVVPGKIYRLR 245
           S P  W+GEPQS+LI G+G+++CS +         C  + +  C+PF+L V P K YR+R
Sbjct: 197 SKPMRWIGEPQSILINGKGQFDCSIAAKYNQGLKQCELSGKEKCAPFILHVQPKKTYRIR 256

Query: 246 IASLTSLSALNFEIEGHNMTVVETDGHFVEPFEVKNLFIYSGETYSVLVTANQDPSRNYW 305
           IAS T+L++LNF I  H + VVE DG++V+PF   ++ IYSGE+YSVL+T +Q+P  NYW
Sbjct: 257 IASTTALASLNFAIGNHELLVVEADGNYVQPFVTSDIDIYSGESYSVLITTDQNPLENYW 316

Query: 306 ATTNVVSRRPGTPTGRAIFNYYPNHPEKNPPTVPTTGPMWNDSTSRVAQSLAIKAREGFI 365
            +  V +R P TP G  + NY PN   K P + P   P W D       +  I A  G  
Sbjct: 317 VSIGVRARLPKTPPGLTLLNYLPNSASKLPISPPPETPHWEDFDRSKNFTFRIFAAMGSP 376

Query: 366 ETPPATSDRVIMLLNTQNRVNGFTKWAVNNVSFTLPTTPYLVALKENRLRNKLHHVFDQS 425
           + PP   +R + LLNTQNR+NGF KWA+NNVS  LP TPYL A+K      +L+  F+Q+
Sbjct: 377 K-PPVRYNRRLFLLNTQNRINGFMKWAINNVSLALPPTPYLAAMKM-----RLNTAFNQN 436

Query: 426 PPPTGYDWLNYDIHVNQSNVNATTSNGIYRLKFNTTIDIVLQNANMINPNNSETHPWHLH 485
           PPP  +  LNYDI+    N   TT NG+Y+     T+D++LQNANM+NPN SE HPWHLH
Sbjct: 437 PPPETFP-LNYDINNPPPNPETTTGNGVYKFNMGETVDVILQNANMLNPNMSEIHPWHLH 496

Query: 486 GHDFWVLGHGVGKFDPFNDPKKYNLVNPILKNTVPLHPYGWTAIRFQANNPGVWLFHCHI 545
           GHDFWVLG+G GKF    D KK NL NP L+NTV + PYGWTAIRF A+NPGVW FHCHI
Sbjct: 497 GHDFWVLGYGEGKFYAPEDEKKLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHI 556

Query: 546 DSHFAMGMGIVFEEGIERVGKLPTSIMGCGLT 573
           + H  MGMG+VF EG+  VG +P   + CG T
Sbjct: 557 EPHLHMGMGVVFAEGVHMVGMIPPKALACGST 577

[Close]

BLAST of Spo10268.1 vs. ExPASy Swiss-Prot
Match: ASO_TOBAC (L-ascorbate oxidase OS=Nicotiana tabacum GN=AAO PE=2 SV=1)

HSP 1 Score: 601.7 bits (1550), Expect = 8.600e-171
Identity = 289/576 (50.17%), Postives = 387/576 (67.19%), Query Frame = 1


		  
Query: 4   YYYVVVMIIFSSLLMSCITVEAAETRYYKWDVKYELKSPDCYRKLVITINGMFPGPTITA 63
           +++++ +I+    L S  +V AA+TR++KWDV+Y   SPD    +V+ ING FPGPTI A
Sbjct: 8   FFFLLPLILLE--LSSSRSVMAAKTRHFKWDVEYIHWSPDGEESVVMGINGQFPGPTIRA 67

Query: 64  EEGDTIVVQVTNSLLTENTAIHWHGIRQIGSPWSDGTEGVTQCPIVPGDTFEYKFVVDRA 123
           + GDT+ V +TN L TE   IHWHGIRQIG+PW+DGT  ++QC I PG+TF Y+F VD+A
Sbjct: 68  KAGDTVAVHLTNKLHTEGVVIHWHGIRQIGTPWADGTAAISQCAINPGETFLYRFKVDKA 127

Query: 124 GTYLYHAHYGMQREEGLNGMIQVKPARGQREPFVYDFDRSIMLTDWYHKTALEHAIGLSS 183
           GTY YH HYGMQR  GL G + V+   G++EPF YD + +++L+DW+HK + E  + LSS
Sbjct: 128 GTYFYHGHYGMQRSAGLYGSLIVEVGEGEKEPFHYDGEFNLLLSDWWHKGSHEQEVDLSS 187

Query: 184 VPFVWVGEPQSLLIQGRGRYNCSSVSIASDPDV--CNATRSD-CSPFVLTVVPGKIYRLR 243
            P  W+GEPQ+LL+ GRG+YNCS  +  S P +  C     +  +P +L V P KIYRLR
Sbjct: 188 NPLRWIGEPQTLLLNGRGQYNCSLAARFSKPPLPQCKLRGGEQYAPQILRVRPNKIYRLR 247

Query: 244 IASLTSLSALNFEIEGHNMTVVETDGHFVEPFEVKNLFIYSGETYSVLVTANQDPSRNYW 303
           +AS T+L +L+  I GH M VVE DG++V+PF V+++ IYSGE+YSVL   +QDP++NYW
Sbjct: 248 VASTTALGSLSLAIGGHKMVVVEADGNYVQPFSVQDMDIYSGESYSVLFKTDQDPTKNYW 307

Query: 304 ATTNVVSRRPGTPTGRAIFNYYPNHPEKNPPTVPTTGPMWNDSTSRVAQSLAIKAREGFI 363
            + NV  R P TP G  + NY PN   K P   P   P+WND     + S  I A  G  
Sbjct: 308 ISINVRGREPKTPQGLTLLNYLPNSASKFPTLPPPIAPLWNDYNHSKSFSNKIFALMGSP 367

Query: 364 ETPPATSDRVIMLLNTQNRVNGFTKWAVNNVSFTLPTTPYLVALKENRLRNKLHHVFDQS 423
           + PP    R+I LLNTQN+++G+TKWA+NNVS  LPT  YL +++         + FD  
Sbjct: 368 KPPPQNHRRII-LLNTQNKIDGYTKWAINNVSLVLPTQLYLGSIRYGI------NAFDTK 427

Query: 424 PPPTGYDWLNYDIHVNQSNVNATTSNGIYRLKFNTTIDIVLQNANMINPNNSETHPWHLH 483
           PPP  +   +YD+     N N+T  NG+Y LKFNTTIDI+LQNAN +  + SE HPWHLH
Sbjct: 428 PPPDNFP-KDYDVLKQAPNSNSTYGNGVYMLKFNTTIDIILQNANALAKDVSEIHPWHLH 487

Query: 484 GHDFWVLGHGVGKFDPFNDPKKYNLVNPILKNTVPLHPYGWTAIRFQANNPGVWLFHCHI 543
           GHDFWVLG+G GKF    D KK+NL NP L+NT  + P+GWTA+RF  +NPGVW FHCHI
Sbjct: 488 GHDFWVLGYGEGKFSE-KDVKKFNLKNPPLRNTAVIFPFGWTALRFVTDNPGVWAFHCHI 547

Query: 544 DSHFAMGMGIVFEEGIERVGKLPTSIMGCGLTKRLI 577
           + H  MGMG++F EG+  V K+P   + CGLT +++
Sbjct: 548 EPHLHMGMGVIFAEGVHLVKKIPKEALACGLTGKML 572

[Close]

BLAST of Spo10268.1 vs. ExPASy Swiss-Prot
Match: ASO_CUCMA (L-ascorbate oxidase OS=Cucurbita maxima GN=AAO PE=1 SV=2)

HSP 1 Score: 594.3 bits (1531), Expect = 1.400e-168
Identity = 278/569 (48.86%), Postives = 376/569 (66.08%), Query Frame = 1


		  
Query: 8   VVMIIFSSLLMSCITVEAAETRYYKWDVKYELKSPDCYRKLVITINGMFPGPTITAEEGD 67
           +++  F  +L   I+ E ++ R+YKW+V+Y   +PDC   +V+ ING FPGPTI A  GD
Sbjct: 13  LILFFFGLILAFGISSEGSQIRHYKWEVEYMFWAPDCNENIVMGINGQFPGPTIRANAGD 72

Query: 68  TIVVQVTNSLLTENTAIHWHGIRQIGSPWSDGTEGVTQCPIVPGDTFEYKFVVDRAGTYL 127
           T+VV++ N L TE   IHWHGI Q G+PW+DGT  ++QC I PG+TF Y F VD  GT+ 
Sbjct: 73  TVVVELINKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGETFFYNFTVDNPGTFF 132

Query: 128 YHAHYGMQREEGLNGMIQVKPARGQREPFVYDFDRSIMLTDWYHKTALEHAIGLSSVPFV 187
           YH H GMQR  GL G + V P +G++EPF YD + +++L+DW+H++  +  +GLSS P  
Sbjct: 133 YHGHLGMQRSAGLYGSLIVDPPQGKKEPFHYDGEINLLLSDWWHQSIHKQEVGLSSKPIR 192

Query: 188 WVGEPQSLLIQGRGRYNCS-SVSIASDPDVCNATRSD-CSPFVLTVVPGKIYRLRIASLT 247
           W+GEPQ++L+ GRG+++CS +    S+ + C    S+ C+P++  V+P K YR+RIAS T
Sbjct: 193 WIGEPQTILLNGRGQFDCSIAAKYDSNLEPCKLKGSEPCAPYIFHVMPKKTYRIRIASTT 252

Query: 248 SLSALNFEIEGHNMTVVETDGHFVEPFEVKNLFIYSGETYSVLVTANQDPSRNYWATTNV 307
           +L+ALNF I  H + VVE DG++V+PF   ++ IYSGE+YSVL+T +Q+PS NYW +   
Sbjct: 253 ALAALNFAIGNHPLLVVEADGNYVQPFYTSDIDIYSGESYSVLITTDQNPSENYWVSVGT 312

Query: 308 VSRRPGTPTGRAIFNYYPNHPEKNPPTVPTTGPMWNDSTSRVAQSLAIKAREGFIETPPA 367
             R P TP G  + NY PN   K P + P   P W+D       +  I A  G    PP 
Sbjct: 313 RGRHPNTPPGLTLLNYLPNSVSKLPTSPPPETPAWDDFDRSKNFTYRITAAMG-SPKPPV 372

Query: 368 TSDRVIMLLNTQNRVNGFTKWAVNNVSFTLPTTPYLVALKENRLRNKLHHVFDQSPPPTG 427
            S+R I LLNTQN +NG+ KWA+N+VS  LP TPYL A+K N L     H FDQ+PPP  
Sbjct: 373 KSNRRIFLLNTQNVINGYVKWAINDVSLALPPTPYLGAMKFNLL-----HAFDQNPPPEV 432

Query: 428 YDWLNYDIHVNQSNVNATTSNGIYRLKFNTTIDIVLQNANMINPNNSETHPWHLHGHDFW 487
           +   +YDI    +N      NG+Y+ K    +D++LQNANM+  N SE HPWHLHGHDFW
Sbjct: 433 FP-EDYDIDTPPTNEKTKIGNGVYQFKIGEIVDVILQNANMMKENLSEIHPWHLHGHDFW 492

Query: 488 VLGHGVGKFDPFNDPKKYNLVNPILKNTVPLHPYGWTAIRFQANNPGVWLFHCHIDSHFA 547
           VLG+G GKF    +    NL NP L+NTV + PYGWTAIRF A+NPGVW FHCHI+ H  
Sbjct: 493 VLGYGDGKFTA-EEESSLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLH 552

Query: 548 MGMGIVFEEGIERVGKLPTSIMGCGLTKR 575
           MGMG+VF EG+E+VG++PT  + CG T +
Sbjct: 553 MGMGVVFAEGVEKVGRIPTKALACGGTAK 573

[Close]

BLAST of Spo10268.1 vs. ExPASy Swiss-Prot
Match: ASO_CUCPM (L-ascorbate oxidase OS=Cucurbita pepo var. melopepo PE=1 SV=1)

HSP 1 Score: 590.5 bits (1521), Expect = 2.000e-167
Identity = 273/551 (49.55%), Postives = 368/551 (66.79%), Query Frame = 1


		  
Query: 26  AETRYYKWDVKYELKSPDCYRKLVITINGMFPGPTITAEEGDTIVVQVTNSLLTENTAIH 85
           ++ R+YKW+V+Y   +P+C   +V+ ING FPGPTI A  GD++VV++TN L TE   IH
Sbjct: 1   SQIRHYKWEVEYMFWAPNCNENIVMGINGQFPGPTIRANAGDSVVVELTNKLHTEGVVIH 60

Query: 86  WHGIRQIGSPWSDGTEGVTQCPIVPGDTFEYKFVVDRAGTYLYHAHYGMQREEGLNGMIQ 145
           WHGI Q G+PW+DGT  ++QC I PG+TF Y F VD  GT+ YH H GMQR  GL G + 
Sbjct: 61  WHGILQRGTPWADGTASISQCAINPGETFFYNFTVDNPGTFFYHGHLGMQRSAGLYGSLI 120

Query: 146 VKPARGQREPFVYDFDRSIMLTDWYHKTALEHAIGLSSVPFVWVGEPQSLLIQGRGRYNC 205
           V P +G++EPF YD + +++L+DW+H++  +  +GLSS P  W+GEPQ++L+ GRG+++C
Sbjct: 121 VDPPQGKKEPFHYDGEINLLLSDWWHQSIHKQEVGLSSKPIRWIGEPQTILLNGRGQFDC 180

Query: 206 S-SVSIASDPDVCNATRSD-CSPFVLTVVPGKIYRLRIASLTSLSALNFEIEGHNMTVVE 265
           S +    S+ + C    S+ C+P++  V P K YR+RIAS T+L+ALNF I  H + VVE
Sbjct: 181 SIAAKYDSNLEPCKLKGSESCAPYIFHVSPKKTYRIRIASTTALAALNFAIGNHQLLVVE 240

Query: 266 TDGHFVEPFEVKNLFIYSGETYSVLVTANQDPSRNYWATTNVVSRRPGTPTGRAIFNYYP 325
            DG++V+PF   ++ IYSGE+YSVL+T +Q+PS NYW +    +R P TP G  + NY P
Sbjct: 241 ADGNYVQPFYTSDIDIYSGESYSVLITTDQNPSENYWVSVGTRARHPNTPPGLTLLNYLP 300

Query: 326 NHPEKNPPTVPTTGPMWNDSTSRVAQSLAIKAREGFIETPPATSDRVIMLLNTQNRVNGF 385
           N   K P + P   P W+D       +  I A  G    PP   +R I LLNTQN +NG+
Sbjct: 301 NSVSKLPTSPPPQTPAWDDFDRSKNFTYRITAAMG-SPKPPVKFNRRIFLLNTQNVINGY 360

Query: 386 TKWAVNNVSFTLPTTPYLVALKENRLRNKLHHVFDQSPPPTGYDWLNYDIHVNQSNVNAT 445
            KWA+N+VS  LP TPYL A+K N L     H FDQ+PPP  +   +YDI    +N    
Sbjct: 361 VKWAINDVSLALPPTPYLGAMKYNLL-----HAFDQNPPPEVFP-EDYDIDTPPTNEKTR 420

Query: 446 TSNGIYRLKFNTTIDIVLQNANMINPNNSETHPWHLHGHDFWVLGHGVGKFDPFNDPKKY 505
             NG+Y+ K    +D++LQNANM+  N SETHPWHLHGHDFWVLG+G GKF    +    
Sbjct: 421 IGNGVYQFKIGEVVDVILQNANMMKENLSETHPWHLHGHDFWVLGYGDGKFSA-EEESSL 480

Query: 506 NLVNPILKNTVPLHPYGWTAIRFQANNPGVWLFHCHIDSHFAMGMGIVFEEGIERVGKLP 565
           NL NP L+NTV + PYGWTAIRF A+NPGVW FHCHI+ H  MGMG+VF EG+E+VG++P
Sbjct: 481 NLKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMGMGVVFAEGVEKVGRIP 540

Query: 566 TSIMGCGLTKR 575
           T  + CG T +
Sbjct: 541 TKALACGGTAK 543

[Close]

BLAST of Spo10268.1 vs. ExPASy Swiss-Prot
Match: LAC22_ORYSJ (Laccase-22 OS=Oryza sativa subsp. japonica GN=LAC22 PE=2 SV=2)

HSP 1 Score: 287.3 bits (734), Expect = 3.600e-76
Identity = 187/560 (33.39%), Postives = 283/560 (50.54%), Query Frame = 1


		  
Query: 16  LLMSCITVEA----AETRYYKWDVKYELKSPDCYRKLVITINGMFPGPTITAEEGDTIVV 75
           L+ +C  ++A    A TR+YK++V     +  C  K ++T+NG FPGPT+ A EGD ++V
Sbjct: 14  LMAACFLLQALSAHAITRHYKFNVVMRNMTRLCSTKPILTVNGKFPGPTLYAREGDNVLV 73

Query: 76  QVTNSLLTENTAIHWHGIRQIGSPWSDGTEGVTQCPIVPGDTFEYKFVVD-RAGTYLYHA 135
           +V N +   N  IHWHG+RQI + W DG   +TQCPI PG +F Y F +  + GT L+HA
Sbjct: 74  KVVNHV-AHNVTIHWHGVRQIRTGWYDGPAYITQCPIQPGSSFLYNFTITGQRGTLLWHA 133

Query: 136 HYGMQREEGLNGMIQVKPARGQREPFVYDFDRS-IMLTDWYHK---TALEHAIGLSSVPF 195
           H    R   ++G I + P  G   PF      + I+L +W+ +   T +  A+ L   P 
Sbjct: 134 HINWLRAT-VHGAIVILPKLGVPYPFPAPHKEAVIVLGEWWKEDTETVINQAMQLGVGPN 193

Query: 196 VWVGEPQSLLIQGRGRYNCSSVSIASDPDVCNATRSDCSPFVLTVVPGKIYRLRIASLTS 255
           +                   S +I   P   +   S    F L+V  GK Y LRI +   
Sbjct: 194 I-----------------SDSHTINGHPGPLSECASSQDGFKLSVENGKTYMLRIINAAL 253

Query: 256 LSALNFEIEGHNMTVVETDGHFVEPFEVKNLFIYSGETYSVLVTANQDPSRNYWATTNVV 315
              L F++ GH +TVVE D  + +PF+   L I  G+T +VLV ANQ   R   + +  +
Sbjct: 254 NDDLFFKVAGHELTVVEVDAVYTKPFKTDTLLITPGQTTNVLVRANQGAGRYLLSVSPFM 313

Query: 316 SR--RPGTPTGRAIFNYYPNHPEKNPPTVPTTGPMWNDST---SRVAQSLAIKAREGFIE 375
               +    TG A   +Y N    +  ++    P   ++T   S+   SL     + +  
Sbjct: 314 DAPVQVDNKTGTATL-HYANTVSSSMASLTLVKPPPQNATHIVSKFTDSLHSLNSKEYPA 373

Query: 376 TPPATSDRVIMLL------NTQNRVNGF-TKWAVNNVSFTLPTTPYLVALKENRLRNKLH 435
             P T D  ++L          + +NG      +NNV+F +P+TP L A   N     + 
Sbjct: 374 NVPQTVDHSLLLTVGVGVNPCPSCINGTRVVGTINNVTFIMPSTPILQAHYYN-----IP 433

Query: 436 HVFDQSPPPTGYDWLNYDIHVNQSNVNATTSNG--IYRLKFNTTIDIVLQNANMINPNNS 495
            VF +  P T     NY         N  T NG  +YRL +N ++ +VLQ+  +I+P   
Sbjct: 434 GVFTEDFPATPLHKFNY---TGSGPKNLQTMNGTRVYRLPYNASVQVVLQDTGIISP--- 493

Query: 496 ETHPWHLHGHDFWVLGHGVGKFDPFNDPKKYNLVNPILKNTVPLHPYGWTAIRFQANNPG 553
           E+HP HLHG +F+V+G GVG ++P   P  +NL++PI +NT+ +   GWTAIRF+++NPG
Sbjct: 494 ESHPIHLHGFNFFVVGKGVGNYNPRTSPSTFNLIDPIERNTIGVPTGGWTAIRFRSDNPG 542

[Close]

BLAST of Spo10268.1 vs. TAIR (Arabidopsis)
Match: AT4G39830.1 (Cupredoxin superfamily protein)

HSP 1 Score: 795.0 bits (2052), Expect = 3.000e-230
Identity = 367/578 (63.49%), Postives = 457/578 (79.07%), Query Frame = 1


		  
Query: 4   YYYVVVMIIFSSLLMSCITVEAAETRYYKWDVKYELKSPDCYRKLVITINGMFPGPTITA 63
           + + ++++ F +L  S +  +  + R +KW+VKYE KSPDC+ KLVITING FPGPTI A
Sbjct: 12  HVFNLMVLCFIALFFSSVLCQG-KIRRFKWEVKYEFKSPDCFEKLVITINGKFPGPTIKA 71

Query: 64  EEGDTIVVQVTNSLLTENTAIHWHGIRQIGSPWSDGTEGVTQCPIVPGDTFEYKFVVDRA 123
           ++GDTIVV++ NS +TEN A+HWHGIRQIG+PW DG EGVTQCPI+PG+ F Y+FVVDR 
Sbjct: 72  QQGDTIVVELKNSFMTENVAVHWHGIRQIGTPWFDGVEGVTQCPILPGEVFIYQFVVDRP 131

Query: 124 GTYLYHAHYGMQREEGLNGMIQVKPARGQREPFVYDFDRSIMLTDWYHKTALEHAIGLSS 183
           GTY+YH+HYGMQRE GL GMIQV P   + EPF YD+DR+ +LTDWYHK+  E A GL+S
Sbjct: 132 GTYMYHSHYGMQRESGLIGMIQVSPPATEPEPFTYDYDRNFLLTDWYHKSMSEKATGLAS 191

Query: 184 VPFVWVGEPQSLLIQGRGRYNCSSVSIASDPD----VCNATRSDCSPFVLTVVPGKIYRL 243
           +PF WVGEPQSL+IQGRGR+NCS+ ++ + P     VCN + +DCS F+LTV+PGK YRL
Sbjct: 192 IPFKWVGEPQSLMIQGRGRFNCSN-NLTTPPSLVSGVCNVSNADCSRFILTVIPGKTYRL 251

Query: 244 RIASLTSLSALNFEIEGHNMTVVETDGHFVEPFEVKNLFIYSGETYSVLVTANQDPSRNY 303
           RI SLT+LSAL+F+IEGHN+TVVE DGH+VEPF VKNLF+YSGETYSVL+ A+Q+P RNY
Sbjct: 252 RIGSLTALSALSFQIEGHNLTVVEADGHYVEPFTVKNLFVYSGETYSVLLKADQNPRRNY 311

Query: 304 WATTNVVSRRPGTPTGRAIFNYYPNHPEKNPPTVPTTG--PMWNDSTSRVAQSLAIKARE 363
           W T+++VSR   TP   A+ NYYPNHP + PPT  ++   P WND+ SR+AQSLAIKAR 
Sbjct: 312 WITSSIVSRPATTPPATAVLNYYPNHPRRRPPTSESSNIVPEWNDTRSRLAQSLAIKARR 371

Query: 364 GFIETPPATSDRVIMLLNTQNRVNGFTKWAVNNVSFTLPTTPYLVALKENRLRNKLHHVF 423
           GFI   P  SD+VI+LLNTQN VNG+ +W+VNNVS+  P TPYL+ALK+N     L + F
Sbjct: 372 GFIHALPENSDKVIVLLNTQNEVNGYRRWSVNNVSYHHPKTPYLIALKQN-----LTNAF 431

Query: 424 D-QSPPPTGYDWLNYDIHVNQSNVNATTSNGIYRLKFNTTIDIVLQNANMINPNNSETHP 483
           D +   P  YD  NYDI     N NATTS+GIYRL+FN+T+D++LQNAN +N NNSETHP
Sbjct: 432 DWRFTAPENYDSRNYDIFAKPLNANATTSDGIYRLRFNSTVDVILQNANTMNANNSETHP 491

Query: 484 WHLHGHDFWVLGHGVGKFDPFNDPKKYNLVNPILKNTVPLHPYGWTAIRFQANNPGVWLF 543
           WHLHGHDFWVLG+G GKF+   DPK+YN V+PI KNTV + P+GWTA+RF+A+NPGVW F
Sbjct: 492 WHLHGHDFWVLGYGEGKFNESEDPKRYNRVDPIKKNTVAVQPFGWTALRFRADNPGVWSF 551

Query: 544 HCHIDSHFAMGMGIVFEEGIERVGKLPTSIMGCGLTKR 575
           HCHI+SHF MGMGIVFE GI++V  LP+SIMGCG TKR
Sbjct: 552 HCHIESHFFMGMGIVFESGIDKVSSLPSSIMGCGQTKR 582

[Close]

BLAST of Spo10268.1 vs. TAIR (Arabidopsis)
Match: AT5G21105.1 (Plant L-ascorbate oxidase)

HSP 1 Score: 574.7 bits (1480), Expect = 6.400e-164
Identity = 279/581 (48.02%), Postives = 374/581 (64.37%), Query Frame = 1


		  
Query: 2   ASYYYVVVMIIFSSLLMSCITVEAAET--RYYKWDVKYELKSPDCYRKLVITINGMFPGP 61
           +S+ Y+  M ++  +L+  +    A    R Y W+V+Y+  SPDC    V+T+NG FPGP
Sbjct: 9   SSFTYISQMGVWWIVLVVAVLTHTASAAVREYHWEVEYKYWSPDCKEGAVMTVNGEFPGP 68

Query: 62  TITAEEGDTIVVQVTNSLLTENTAIHWHGIRQIGSPWSDGTEGVTQCPIVPGDTFEYKFV 121
           TI A  GDTIVV +TN L TE   IHWHGIRQ GSPW+DG  GVTQC I PG+TF Y F 
Sbjct: 69  TIKAFAGDTIVVNLTNKLTTEGLVIHWHGIRQFGSPWADGAAGVTQCAINPGETFTYNFT 128

Query: 122 VDRAGTYLYHAHYGMQREEGLNGMIQVKPARGQREPFVYDFDRSIMLTDWYHKTALEHAI 181
           V++ GT+ YH HYGMQR  GL G + V  A+G+ E   YD + +++L+DW+H+      +
Sbjct: 129 VEKPGTHFYHGHYGMQRSAGLYGSLIVDVAKGKSERLRYDGEFNLLLSDWWHEAIPSQEL 188

Query: 182 GLSSVPFVWVGEPQSLLIQGRGRYNCSSVSIASDPD---VCNATRSD-CSPFVLTVVPGK 241
           GLSS P  W+GE QS+LI GRG++NCS  +  S+     +C     D C+P +L V P K
Sbjct: 189 GLSSKPMRWIGEAQSILINGRGQFNCSLAAQFSNNTSLPMCTFKEGDQCAPQILHVEPNK 248

Query: 242 IYRLRIASLTSLSALNFEIEGHNMTVVETDGHFVEPFEVKNLFIYSGETYSVLVTANQDP 301
            YR+R++S T+L++LN  ++GH + VVE DG+++ PF   ++ IYSGE+YSVL+T +QDP
Sbjct: 249 TYRIRLSSTTALASLNLAVQGHKLVVVEADGNYITPFTTDDIDIYSGESYSVLLTTDQDP 308

Query: 302 SRNYWATTNVVSRRPGTPTGRAIFNYYPNHPEKNPPTVPTTGPMWNDSTSRVAQSLAIKA 361
           S+NY+ +  V  R+P T     I NY      K P + P   P W+D       S  I +
Sbjct: 309 SQNYYISVGVRGRKPNTTQALTILNYVTAPASKLPSSPPPVTPRWDDFERSKNFSKKIFS 368

Query: 362 REGFIETPPATSDRVIMLLNTQNRVNGFTKWAVNNVSFTLPTTPYLVALKENRLRNKLHH 421
             G   +PP    + ++LLNTQN ++G+TKWA+NNVS   P TPYL ++K N     L  
Sbjct: 369 AMGS-PSPPKKYRKRLILLNTQNLIDGYTKWAINNVSLVTPATPYLGSVKYN-----LKL 428

Query: 422 VFDQSPPPTGYDWLNYDIHVNQSNVNATTSNGIYRLKFNTTIDIVLQNANMINPNNSETH 481
            F++  PP  Y  ++YDI       N TT NGIY   FN T+D+++QNAN++    SE H
Sbjct: 429 GFNRKSPPRSYR-MDYDIMNPPPFPNTTTGNGIYVFPFNVTVDVIIQNANVLKGIVSEIH 488

Query: 482 PWHLHGHDFWVLGHGVGKFDPFNDPKKYNLVNPILKNTVPLHPYGWTAIRFQANNPGVWL 541
           PWHLHGHDFWVLG+G GKF P  D K YNL NP L+NT  L+PYGWTAIRF  +NPGVW 
Sbjct: 489 PWHLHGHDFWVLGYGDGKFKPGIDEKTYNLKNPPLRNTAILYPYGWTAIRFVTDNPGVWF 548

Query: 542 FHCHIDSHFAMGMGIVFEEGIERVGKLPTSIMGCGLTKRLI 577
           FHCHI+ H  MGMG+VF EG+ R+GK+P   +GCGLTK+ +
Sbjct: 549 FHCHIEPHLHMGMGVVFAEGLNRIGKVPDEALGCGLTKQFL 582

[Close]

BLAST of Spo10268.1 vs. TAIR (Arabidopsis)
Match: AT5G21100.1 (Plant L-ascorbate oxidase)

HSP 1 Score: 569.7 bits (1467), Expect = 2.100e-162
Identity = 276/573 (48.17%), Postives = 368/573 (64.22%), Query Frame = 1


		  
Query: 8   VVMIIFSSLLMSCITVEAAETRYYKWDVKYELKSPDCYRKLVITINGMFPGPTITAEEGD 67
           VV++ F S   S   VE+       W+V+Y+   PDC   +V+ ING FPGPTI A  GD
Sbjct: 11  VVVVAFHSA--SAAVVEST------WEVEYKYWWPDCKEGIVMAINGQFPGPTIDAVAGD 70

Query: 68  TIVVQVTNSLLTENTAIHWHGIRQIGSPWSDGTEGVTQCPIVPGDTFEYKFVVDRAGTYL 127
           T+++ V N L TE   IHWHGIRQ G+PW+DG  GVTQCPI PG+TF YKF+VD+AGT+ 
Sbjct: 71  TVIIHVVNKLSTEGVVIHWHGIRQKGTPWADGAAGVTQCPINPGETFTYKFIVDKAGTHF 130

Query: 128 YHAHYGMQREEGLNGMIQVKPARGQREPFVYDFDRSIMLTDWYHKTALEHAIGLSSVPFV 187
           YH HYGMQR  GL GM+ V   R  +E  +YD + +++L+DW+H++     + LSS P  
Sbjct: 131 YHGHYGMQRSSGLYGMLIV---RSPKERLIYDGEFNLLLSDWWHQSIHAQELALSSRPMR 190

Query: 188 WVGEPQSLLIQGRGRYNCSSVSI---ASDPDVCNATRSD-CSPFVLTVVPGKIYRLRIAS 247
           W+GEPQSLLI GRG++NCS  +      + DVC    +D C+P  L V P ++YRLRIAS
Sbjct: 191 WIGEPQSLLINGRGQFNCSQAAYFNKGGEKDVCTFKENDQCAPQTLRVEPNRVYRLRIAS 250

Query: 248 LTSLSALNFEIEGHNMTVVETDGHFVEPFEVKNLFIYSGETYSVLVTANQDPSRNYWATT 307
            T+L++LN  ++GH + VVE DG++V PF V ++ +YSGETYSVL+  N  PS+ YW + 
Sbjct: 251 TTALASLNLAVQGHQLVVVEADGNYVAPFTVNDIDVYSGETYSVLLKTNALPSKKYWISV 310

Query: 308 NVVSRRPGTPTGRAIFNYYPNHPEKNPPTVPTTGPMWNDSTSRVAQSLAIKAREGFIETP 367
            V  R P TP    + NY  +  E  P   P   P+WND+    + S  I A +G+ + P
Sbjct: 311 GVRGREPKTPQALTVINYV-DATESRPSHPPPVTPIWNDTDRSKSFSKKIFAAKGYPKPP 370

Query: 368 PATSDRVIMLLNTQNRVNGFTKWAVNNVSFTLPTTPYLVALKENRLRNKLHHVFDQSPPP 427
             + D++I LLNTQN    +TKW++NNVS ++P TPYL       +R  L   +D   P 
Sbjct: 371 EKSHDQLI-LLNTQNLYEDYTKWSINNVSLSVPVTPYL-----GSIRYGLKSAYDLKSPA 430

Query: 428 TGYDWLNYDIHVNQSNVNATTSNGIYRLKFNTTIDIVLQNANMINPNNSETHPWHLHGHD 487
                 NYDI     N N T  +GIY   F   +D++LQNAN++    SE HPWH+HGHD
Sbjct: 431 KKLIMDNYDIMKPPPNPNTTKGSGIYNFAFGIVVDVILQNANVLKGVISEIHPWHIHGHD 490

Query: 488 FWVLGHGVGKFDPFNDPKKYNLVNPILKNTVPLHPYGWTAIRFQANNPGVWLFHCHIDSH 547
           FWVLG+G GKF P  D K +NL NP L+NTV L+P+GWTAIRF  +NPGVW FHCHI+ H
Sbjct: 491 FWVLGYGEGKFKPGIDEKTFNLKNPPLRNTVVLYPFGWTAIRFVTDNPGVWFFHCHIEPH 550

Query: 548 FAMGMGIVFEEGIERVGK--LPTSIMGCGLTKR 575
             MGMG+VF EG++R+GK  +P   +GCGLT++
Sbjct: 551 LHMGMGVVFVEGVDRIGKMEIPDEALGCGLTRK 565

[Close]

BLAST of Spo10268.1 vs. TAIR (Arabidopsis)
Match: AT5G03260.1 (laccase 11)

HSP 1 Score: 285.0 bits (728), Expect = 1.000e-76
Identity = 191/543 (35.17%), Postives = 282/543 (51.93%), Query Frame = 1


		  
Query: 26  AETRYYKWDVKYELKSPDCYRKLVITINGMFPGPTITAEEGDTIVVQVTNSLLTENTAIH 85
           A  + Y++DV+ +  S  C  K ++T+NGMFPGPT+ A EGD +++ VTN +   N +IH
Sbjct: 23  AAVKKYQFDVQVKNISRICNAKPIVTVNGMFPGPTVYAREGDRVIINVTNHV-QYNMSIH 82

Query: 86  WHGIRQIGSPWSDGTEGVTQCPIVPGDTFEYKF-VVDRAGTYLYHAHYGMQREEGLNGMI 145
           WHG++Q  + W+DG   +TQCPI  G ++ Y F V  + GT  +HAH    R   + G I
Sbjct: 83  WHGLKQYRNGWADGPAYITQCPIQTGQSYLYDFNVTGQRGTLWWHAHILWLRAT-VYGAI 142

Query: 146 QVKPARGQREPFVYDFDRS-IMLTDWYHK---TALEHAIGLSSVPFVWVGEPQSLLIQGR 205
            + PA G+  PF   +  S I+L +W++K   TA+  A  L + P      P S      
Sbjct: 143 VILPAPGKPYPFPQPYQESNIILGEWWNKDVETAVNQANQLGAPP------PMS------ 202

Query: 206 GRYNCSSVSIASDPDVCNATRSDCSPFVLTVVPGKIYRLRIASLTSLSALNFEIEGHNMT 265
                 + +I   P       S+   FV+    GK Y LRI +      L F I GHNMT
Sbjct: 203 -----DAHTINGKPGPLFPC-SEKHTFVIEAEAGKTYLLRIINAALNDELFFGIAGHNMT 262

Query: 266 VVETDGHFVEPFEVKNLFIYSGETYSVLVTANQDPSRNYWATTNVVSRRPGTP--TGRAI 325
           VVE D  + +PF  K + +  G+T +VLV  ++ P+R + A +  +         T  AI
Sbjct: 263 VVEIDAVYTKPFTTKAILLGPGQTTNVLVKTDRSPNRYFMAASPFMDAPVSVDNKTVTAI 322

Query: 326 FNYYPNHPEKNPPTVPTTGPMWNDSTSRVAQSLAIKARE--GFIETPPATSDRVIML--- 385
             Y    P    P +P   P+ ND++  +  +  +K+     F    P   DR +     
Sbjct: 323 LQY-KGVPNTVLPILPKL-PLPNDTSFALDYNGKLKSLNTPNFPALVPLKVDRRLFYTIG 382

Query: 386 --LNT-QNRVNGFTKWA-VNNVSFTLPTTPYLVALKENRLRNKLHHVFDQSPPPTGYDWL 445
             +N     VNG    A +NN++F +P T  L A   N +       F   PP       
Sbjct: 383 LGINACPTCVNGTNLAASINNITFIMPKTALLKAHYSN-ISGVFRTDFPDRPPKA----F 442

Query: 446 NYDIHVNQSNVNATTSNGIYRLKFNTTIDIVLQNANMINPNNSETHPWHLHGHDFWVLGH 505
           NY      +N+  +T   + R+KFNTTI++VLQ+ N++     E+HP+HLHG++F+V+G 
Sbjct: 443 NYTGVPLTANLGTSTGTRLSRVKFNTTIELVLQDTNLLTV---ESHPFHLHGYNFFVVGT 502

Query: 506 GVGKFDPFNDPKKYNLVNPILKNTVPLHPYGWTAIRFQANNPGVWLFHCHIDSHFAMGMG 553
           GVG FDP  DP K+NLV+P  +NTV +   GW AIRF+A+NPGVW  HCH++ H   G+ 
Sbjct: 503 GVGNFDPKKDPAKFNLVDPPERNTVGVPTGGWAAIRFRADNPGVWFMHCHLEVHTMWGLK 535

[Close]

BLAST of Spo10268.1 vs. TAIR (Arabidopsis)
Match: AT5G05390.1 (laccase 12)

HSP 1 Score: 270.4 bits (690), Expect = 2.600e-72
Identity = 175/573 (30.54%), Postives = 282/573 (49.21%), Query Frame = 1


		  
Query: 1   MASYYYVVVMIIFSSLLMSCITVEAAETRYYKWDVKYELKSPDCYRKLVITINGMFPGPT 60
           M + +   +++ F SL  + + +  A+ +++ + ++       C  +  IT+NGMFPGPT
Sbjct: 1   MTTVHTFSILLFFCSLFSASLII--AKVQHHDFVIQETPVKRLCKTRNAITVNGMFPGPT 60

Query: 61  ITAEEGDTIVVQVTNSLLTENTAIHWHGIRQIGSPWSDGTEGVTQCPIVPGDTFEYKFVV 120
           +    GDT+ V+V N     N  IHWHG+RQI + W+DG E VTQCPI PG ++ Y+F +
Sbjct: 61  LEVNNGDTLEVKVHNRA-RYNITIHWHGVRQIRTGWADGPEFVTQCPIRPGKSYTYRFTI 120

Query: 121 D-RAGTYLYHAHYGMQREEGLNGMIQVKPARGQREPFVY-DFDRSIMLTDWYHKTALE-- 180
             + GT  +HAH    R   + G + + P  G   PF   D   ++ML +W++   ++  
Sbjct: 121 QGQEGTLWWHAHSSWLRAT-VYGALIIHPTPGSSFPFPKPDRQTALMLGEWWNANPVDVI 180

Query: 181 -HAIGLSSVPFVWVGEPQSLLIQGRGRYNCSSVSIASDPDVCNATRSDCSPFVLTVVPGK 240
             A    + P +   +  ++  Q    YNCS+                    V+ +  G+
Sbjct: 181 NQATRTGAAPNI--SDAYTINGQPGDLYNCSTKETV----------------VVPINSGE 240

Query: 241 IYRLRIASLTSLSALNFEIEGHNMTVVETDGHFVEPFEVKNLFIYSGETYSVLVTANQDP 300
              LR+ +      L F +  H +TVV  D  +++PF  K L +  G+T  VL+TA+Q P
Sbjct: 241 TSLLRVINAALNQPLFFTVANHKLTVVGADASYLKPFTTKVLMLGPGQTTDVLLTADQPP 300

Query: 301 SRNYWATTNVVSRR--PGTPTGRAIFNYYPNHPEKNPPTVPTTGPMWNDSTSRVAQSLAI 360
            R Y A     S +  P   T       Y      + P +P   P +ND+ +  + S   
Sbjct: 301 KRYYIAARAYQSAQNAPFDNTTTTAILQYKKTTTTSKPIMPVL-PAFNDTNTVTSFSRKF 360

Query: 361 KAREGFIETPPATSDRVIMLLNT----------QNRVNGF--TKW--AVNNVSFTLPTTP 420
           K+    +  P    D +   +            ++R  G   T++  ++NNVSF LP+  
Sbjct: 361 KSLRNVV-VPKTIDDNLFFTIGLGLDNCPKKFPKSRCQGLNGTRFTASMNNVSFVLPSNF 420

Query: 421 YLVALKENRLRNKLHHVFDQSPPPTGYDWLNYDIHVNQSNVNATTSNGIYRLKFNTTIDI 480
            L+    N +       F  S PP  +D+   +I  +++         +Y+LK+ + + +
Sbjct: 421 SLLQAHSNGIPGVFTTDFP-SKPPVKFDYTGNNI--SRALFQPVKGTKLYKLKYGSRVQV 480

Query: 481 VLQNANMINPNNSETHPWHLHGHDFWVLGHGVGKFDPFNDPKKYNLVNPILKNTVPLHPY 540
           VLQ+ N++    SE HP HLHG+DF+++G G G F+P  D  K+NLV+P L+NTV +   
Sbjct: 481 VLQDTNIVT---SENHPIHLHGYDFYIVGEGFGNFNPKKDTSKFNLVDPPLRNTVAVPVN 540

Query: 541 GWTAIRFQANNPGVWLFHCHIDSHFAMGMGIVF 553
           GW  IRF A+NPGVWL HCH+D H   G+ + F
Sbjct: 541 GWAVIRFVADNPGVWLMHCHLDVHIKWGLAMAF 543

The following BLAST results are available for this feature:

BLAST of Spo10268.1 vs. NCBI nr
BLAST of Spo10268.1 vs. UniProtKB/TrEMBL
BLAST of Spo10268.1 vs. ExPASy Swiss-Prot
BLAST of Spo10268.1 vs. TAIR (Arabidopsis)

BLAST of Spo10268.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902035835\|gb\|KNA02633.1\|	0.0e+0	100.	hypothetical protein SOVF_2168...	[more]
gi\|731338940\|ref\|XP_010680584.1\|	3.6e-294	82.4	PREDICTED: L-ascorbate oxidase...	[more]
gi\|870857268\|gb\|KMT08828.1\|	4.4e-292	83.1	hypothetical protein BVRB_6g13...	[more]
gi\|743923090\|ref\|XP_011005626.1\|	1.1e-253	71.8	PREDICTED: L-ascorbate oxidase...	[more]
gi\|566179811\|ref\|XP_006380435.1\|	1.4e-253	72.2	L-ascorbate oxidase family pro...	[more]

BLAST of Spo10268.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9Q7I6_SPIOL	0.0e+0	100.	Uncharacterized protein OS=Spi...	[more]
A0A0J8C9Y3_BETVU	3.1e-292	83.1	Uncharacterized protein OS=Bet...	[more]
U5G9V4_POPTR	9.7e-254	72.2	L-ascorbate oxidase family pro...	[more]
M5X9Y5_PRUPE	3.1e-252	70.8	Uncharacterized protein OS=Pru...	[more]
B9HFP2_POPTR	9.1e-252	71.6	Uncharacterized protein OS=Pop...	[more]

BLAST of Spo10268.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
ASO_CUCSA	2.3e-171	50.7	L-ascorbate oxidase OS=Cucumis...	[more]
ASO_TOBAC	8.6e-171	50.1	L-ascorbate oxidase OS=Nicotia...	[more]
ASO_CUCMA	1.4e-168	48.8	L-ascorbate oxidase OS=Cucurbi...	[more]
ASO_CUCPM	2.0e-167	49.5	L-ascorbate oxidase OS=Cucurbi...	[more]
LAC22_ORYSJ	3.6e-76	33.3	Laccase-22 OS=Oryza sativa sub...	[more]

BLAST of Spo10268.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 5


Match Name	E-value	Identity	Description
AT4G39830.1	3.0e-230	63.4	Cupredoxin superfamily protein	[more]
AT5G21105.1	6.4e-164	48.0	Plant L-ascorbate oxidase	[more]
AT5G21100.1	2.1e-162	48.1	Plant L-ascorbate oxidase	[more]
AT5G03260.1	1.0e-76	35.1	laccase 11	[more]
AT5G05390.1	2.6e-72	30.5	laccase 12	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001117	Multicopper oxidase, type 1	PFAM	PF00394	Cu-oxidase	coord: 162..323 score: 9.2
IPR002355	Multicopper oxidase, copper-binding site	PROSITE	PS00080	MULTICOPPER_OXIDASE2	coord: 537..548 scor
IPR008972	Cupredoxin	GENE3D	2.60.40.420		coord: 166..347 score: 3.8E-54coord: 41..165 score: 5.5E-37coord: 389..571 score: 1.8
IPR008972	Cupredoxin	unknown	SSF49503	Cupredoxins	coord: 364..569 score: 1.86E-46coord: 155..343 score: 2.83E-45coord: 26..172 score: 3.77
IPR011706	Multicopper oxidase, type 2	PFAM	PF07731	Cu-oxidase_2	coord: 439..555 score: 7.8
IPR011707	Multicopper oxidase, type 3	PFAM	PF07732	Cu-oxidase_3	coord: 35..149 score: 1.4
IPR017760	L-ascorbate oxidase, plants	TIGRFAMs	TIGR03388	TIGR03388	coord: 29..572 score: 1.3E
None	No IPR available	PANTHER	PTHR11709	MULTI-COPPER OXIDASE	coord: 1..578 score:
None	No IPR available	PANTHER	PTHR11709:SF28	SUBFAMILY NOT NAMED	coord: 1..578 score:

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
biological_process	GO:0019852	L-ascorbic acid metabolic process
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0005507	copper ion binding
molecular_function	GO:0016491	oxidoreductase activity
molecular_function	GO:0008447	L-ascorbate oxidase activity