Spo10773.1 (mRNA) | SpinachBase

Overview


Name	Spo10773.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	Kunitz trypsin inhibitor
Location	chr1 : 41391970 .. 41392596 (-)
Sequence length	627

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACTAATTTTATCCTCTCAGCAGCCACCTTTCTCTTCTTCCTCCTTTCCTCTCCTCCATCCACCACCGCGGCCGCAAAAATAACCCCGGTCTTCGATATCAACGGCCGCCCTCTCCAAACCGGCTCCACCTACTACATCCTTCCCGTGATCCGAGGCCGAGGCGGGGGCCTTACAATGACACCCATAAACACCACACAATCATGCCCACTTTACGTAGCCCAAGAAAACCACGAAGTCTCAAACGGCCTACCTCTTAAAATCTACCCGGTCAACCCAAATGAGAAAAGAGTCCCTTTAGGGGGTGACGTCAACATCGTCTTCGATGCCGCCTCCATCTGCGTACAATCCACCGGATGGATGCTGGCCTTCGACGAGGCCACCGGAAAACAACACGTGGCGACTGGAGGAACAATCGGAAACCCGGGTGTCGATACCTTGAGTAACTGGTTCCGGATTGAGAAGGCTGGGAGTGGGATGTATGATTATAAGGTGTCGTTTTGCCCAGGTGTTTGTATCTTTTGTGCGGTCATGTGTGGGGATGTTGGTGTTTTTATTGGGGAAGATGGCACTAGGTTTCTTGGTCTTACCGATCGTCCTCTTTTGGTTAAGTTCAAGAAAGCTTAA

mRNA sequence

ATGACTAATTTTATCCTCTCAGCAGCCACCTTTCTCTTCTTCCTCCTTTCCTCTCCTCCATCCACCACCGCGGCCGCAAAAATAACCCCGGTCTTCGATATCAACGGCCGCCCTCTCCAAACCGGCTCCACCTACTACATCCTTCCCGTGATCCGAGGCCGAGGCGGGGGCCTTACAATGACACCCATAAACACCACACAATCATGCCCACTTTACGTAGCCCAAGAAAACCACGAAGTCTCAAACGGCCTACCTCTTAAAATCTACCCGGTCAACCCAAATGAGAAAAGAGTCCCTTTAGGGGGTGACGTCAACATCGTCTTCGATGCCGCCTCCATCTGCGTACAATCCACCGGATGGATGCTGGCCTTCGACGAGGCCACCGGAAAACAACACGTGGCGACTGGAGGAACAATCGGAAACCCGGGTGTCGATACCTTGAGTAACTGGTTCCGGATTGAGAAGGCTGGGAGTGGGATGTATGATTATAAGGTGTCGTTTTGCCCAGGTGTTTGTATCTTTTGTGCGGTCATGTGTGGGGATGTTGGTGTTTTTATTGGGGAAGATGGCACTAGGTTTCTTGGTCTTACCGATCGTCCTCTTTTGGTTAAGTTCAAGAAAGCTTAA

Coding sequence (CDS)

ATGACTAATTTTATCCTCTCAGCAGCCACCTTTCTCTTCTTCCTCCTTTCCTCTCCTCCATCCACCACCGCGGCCGCAAAAATAACCCCGGTCTTCGATATCAACGGCCGCCCTCTCCAAACCGGCTCCACCTACTACATCCTTCCCGTGATCCGAGGCCGAGGCGGGGGCCTTACAATGACACCCATAAACACCACACAATCATGCCCACTTTACGTAGCCCAAGAAAACCACGAAGTCTCAAACGGCCTACCTCTTAAAATCTACCCGGTCAACCCAAATGAGAAAAGAGTCCCTTTAGGGGGTGACGTCAACATCGTCTTCGATGCCGCCTCCATCTGCGTACAATCCACCGGATGGATGCTGGCCTTCGACGAGGCCACCGGAAAACAACACGTGGCGACTGGAGGAACAATCGGAAACCCGGGTGTCGATACCTTGAGTAACTGGTTCCGGATTGAGAAGGCTGGGAGTGGGATGTATGATTATAAGGTGTCGTTTTGCCCAGGTGTTTGTATCTTTTGTGCGGTCATGTGTGGGGATGTTGGTGTTTTTATTGGGGAAGATGGCACTAGGTTTCTTGGTCTTACCGATCGTCCTCTTTTGGTTAAGTTCAAGAAAGCTTAA

Protein sequence

MTNFILSAATFLFFLLSSPPSTTAAAKITPVFDINGRPLQTGSTYYILPVIRGRGGGLTMTPINTTQSCPLYVAQENHEVSNGLPLKIYPVNPNEKRVPLGGDVNIVFDAASICVQSTGWMLAFDEATGKQHVATGGTIGNPGVDTLSNWFRIEKAGSGMYDYKVSFCPGVCIFCAVMCGDVGVFIGEDGTRFLGLTDRPLLVKFKKA

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo10773	Spo10773	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo10773.1	Spo10773.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo10773.1.CDS.1	Spo10773.1.CDS.1	CDS

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo10773.1.exon.1	Spo10773.1.exon.1	exon

Homology

[Close]

BLAST of Spo10773.1 vs. NCBI nr
Match: gi|902170260|gb|KNA07819.1| (hypothetical protein SOVF_168350 [Spinacia oleracea])

HSP 1 Score: 428.3 bits (1100), Expect = 7.500e-117
Identity = 207/208 (99.52%), Postives = 207/208 (99.52%), Query Frame = 1


		  
Query: 1   MTNFILSAATFLFFLLSSPPSTTAAAKITPVFDINGRPLQTGSTYYILPVIRGRGGGLTM 60
           MTNFILSAATFLFFLLSSPPSTTAAAKITPVFDINGRPLQTGSTYYILPVIRGRGGGLTM
Sbjct: 1   MTNFILSAATFLFFLLSSPPSTTAAAKITPVFDINGRPLQTGSTYYILPVIRGRGGGLTM 60

Query: 61  TPINTTQSCPLYVAQENHEVSNGLPLKIYPVNPNEKRVPLGGDVNIVFDAASICVQSTGW 120
           TPINTTQSCPLYVAQENHEVSNGLPLKIYPVNPNEKRVPLGGDVNIVFDAASICVQSTGW
Sbjct: 61  TPINTTQSCPLYVAQENHEVSNGLPLKIYPVNPNEKRVPLGGDVNIVFDAASICVQSTGW 120

Query: 121 MLAFDEATGKQHVATGGTIGNPGVDTLSNWFRIEKAGSGMYDYKVSFCPGVCIFCAVMCG 180
           MLAFDEATGKQHVATGGTIGNPGVDTLSNWFRIEKAGSGMYDYKVSFCPGVCIFC VMCG
Sbjct: 121 MLAFDEATGKQHVATGGTIGNPGVDTLSNWFRIEKAGSGMYDYKVSFCPGVCIFCTVMCG 180

Query: 181 DVGVFIGEDGTRFLGLTDRPLLVKFKKA 209
           DVGVFIGEDGTRFLGLTDRPLLVKFKKA
Sbjct: 181 DVGVFIGEDGTRFLGLTDRPLLVKFKKA 208

[Close]

BLAST of Spo10773.1 vs. NCBI nr
Match: gi|731344029|ref|XP_010683192.1| (PREDICTED: miraculin-like [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 329.7 bits (844), Expect = 3.600e-87
Identity = 161/206 (78.16%), Postives = 175/206 (84.95%), Query Frame = 1


		  
Query: 5   ILSAATFLFFLLSSPPSTT-AAAKITPVFDINGRPLQTGSTYYILPVIRGRGGGLTMTPI 64
           ILS  T L FLL +P STT AAAK T V DINGRPL+T STYYILPV RGRGGGL M P 
Sbjct: 6   ILSILTILLFLLIAPLSTTTAAAKTTTVLDINGRPLKTDSTYYILPVSRGRGGGLAMAPK 65

Query: 65  NTTQSCPLYVAQENHEVSNGLPLKIYPVNPNEK-RVPLGGDVNIVFDAASICVQSTGWML 124
           N T+SCPLYVAQENHEVSNGLPLK +P NPN+  ++PLG DVNIVFDAA+ICVQ TGWML
Sbjct: 66  NATESCPLYVAQENHEVSNGLPLKFFPTNPNDHDKIPLGYDVNIVFDAATICVQPTGWML 125

Query: 125 AFDEATGKQHVATGGTIGNPGVDTLSNWFRIEKAGSGMYDYKVSFCPGVCIFCAVMCGDV 184
           AFDEA+G ++V  GGTIGNPGVDTLSNWF IEKAGSG+YDYK+ FCP VCIFC VMCGDV
Sbjct: 126 AFDEASGTRYVGIGGTIGNPGVDTLSNWFAIEKAGSGLYDYKIRFCPAVCIFCTVMCGDV 185

Query: 185 GVFIGEDGTRFLGLTDRPLLVKFKKA 209
           GVFIGE GTRFL LTDRPLLV+FKKA
Sbjct: 186 GVFIGEGGTRFLALTDRPLLVRFKKA 211

[Close]

BLAST of Spo10773.1 vs. NCBI nr
Match: gi|731344031|ref|XP_010683193.1| (PREDICTED: miraculin-like [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 293.5 bits (750), Expect = 2.900e-76
Identity = 140/208 (67.31%), Postives = 161/208 (77.40%), Query Frame = 1


		  
Query: 1   MTNFILSAATFLFFLLSSPPSTTAAAKITPVFDINGRPLQTGSTYYILPVIRGRGGGLTM 60
           MT+ I+SAAT   FL  SP   T+ A  T V DINGRPLQ G  YYILPVIRGRGGGLTM
Sbjct: 1   MTHVIISAATIFLFLYLSP--LTSTADNTAVLDINGRPLQAGYNYYILPVIRGRGGGLTM 60

Query: 61  TPINTTQSCPLYVAQENHEVSNGLPLKIYPVNPNEKRVPLGGDVNIVFDAASICVQSTGW 120
              N T+ CPLYVAQE+HEVSNGLPLK YPVNP +KR+ L  D+N VFDAA+ CVQSTGW
Sbjct: 61  ASKNATELCPLYVAQEDHEVSNGLPLKFYPVNPKDKRISLSSDLNFVFDAATTCVQSTGW 120

Query: 121 MLAFDEATGKQHVATGGTIGNPGVDTLSNWFRIEKAGSGMYDYKVSFCPGVCIFCAVMCG 180
            L  +  TG+++V TGG IGNPGV+T+ NWF+IEK GSG YDYK+ +CPGVC FC VMCG
Sbjct: 121 SLTIEMETGRRYVGTGGEIGNPGVETVDNWFKIEKDGSGKYDYKIVYCPGVCNFCKVMCG 180

Query: 181 DVGVFIGEDGTRFLGLTDRPLLVKFKKA 209
           DVGVFI +DG R LG +D+PLLV FKKA
Sbjct: 181 DVGVFIEKDGRRLLGFSDQPLLVMFKKA 206

[Close]

BLAST of Spo10773.1 vs. NCBI nr
Match: gi|902194163|gb|KNA12470.1| (hypothetical protein SOVF_125760 [Spinacia oleracea])

HSP 1 Score: 282.0 bits (720), Expect = 8.700e-73
Identity = 132/208 (63.46%), Postives = 159/208 (76.44%), Query Frame = 1


		  
Query: 1   MTNFILSAATFLFFLLSSPPSTTAAAKITPVFDINGRPLQTGSTYYILPVIRGRGGGLTM 60
           M ++ LSAAT L FL  SPP+TTAA     V DING PL+  S YYILPV+RGRGGGLTM
Sbjct: 1   MIHYFLSAATILLFLSLSPPTTTAANPA--VLDINGHPLRPRSNYYILPVVRGRGGGLTM 60

Query: 61  TPINTTQSCPLYVAQENHEVSNGLPLKIYPVNPNEKRVPLGGDVNIVFDAASICVQSTGW 120
              NTT+ CPLYVAQENHEVSNGLPLK Y VNP + ++ +  D+N VFDA++ICVQS  W
Sbjct: 61  LSKNTTEVCPLYVAQENHEVSNGLPLKFYSVNPKDTKIFVSTDLNFVFDASTICVQSNTW 120

Query: 121 MLAFDEATGKQHVATGGTIGNPGVDTLSNWFRIEKAGSGMYDYKVSFCPGVCIFCAVMCG 180
            L  DE TG++++  GG IGNPGV T+SNWF+IEKAGSG YDYK+ +C GVC FC +MCG
Sbjct: 121 ELTIDEETGRRYIGVGGEIGNPGVQTVSNWFKIEKAGSGKYDYKIVYCAGVCNFCKIMCG 180

Query: 181 DVGVFIGEDGTRFLGLTDRPLLVKFKKA 209
           D+G F+ EDG R LG + +PLLV+FKKA
Sbjct: 181 DIGAFVEEDGRRLLGFSGQPLLVRFKKA 206

[Close]

BLAST of Spo10773.1 vs. NCBI nr
Match: gi|731344033|ref|XP_010683194.1| (PREDICTED: miraculin-like [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 275.8 bits (704), Expect = 6.200e-71
Identity = 135/205 (65.85%), Postives = 158/205 (77.07%), Query Frame = 1


		  
Query: 5   ILSAATFLFFLLSSPPSTTAAAKITPVFDINGRPLQTGSTYYILPVIRGRGGGLTMTPIN 64
           ILSAAT   FL  SP ++TAA     V DINGRPLQ  S YYILPVIRGRGGGL MTP N
Sbjct: 6   ILSAATIFLFLCLSPLTSTAAN--IAVLDINGRPLQARSNYYILPVIRGRGGGLRMTPKN 65

Query: 65  TTQSCPL-YVAQENHEVSNGLPLKIYPVNPNEKRVPLGGDVNIVFDAASICVQSTGWMLA 124
            TQ CPL YVAQE  E++NGLPLK YPVNP +K + L  D+N VFDAA+ICVQST W LA
Sbjct: 66  ATQLCPLAYVAQEGSELANGLPLKFYPVNPKDKTISLSTDLNFVFDAATICVQSTQWRLA 125

Query: 125 FDEATGKQHVATGGTIGNPGVDTLSNWFRIEKAGSGMYDYKVSFCPGVCIFCAVMCGDVG 184
           FDE TG+++V  GG IGNPG +T+SNWF+IEKA +G YDYK+ FCPGVC FC V CG++G
Sbjct: 126 FDEVTGRRYVGFGGEIGNPGGNTVSNWFKIEKAETGKYDYKIVFCPGVCNFCKVACGEIG 185

Query: 185 VFIGEDGTRFLGLTDRPLLVKFKKA 209
           VF+ +DG R LG+ ++PLLV FKKA
Sbjct: 186 VFVEKDGRRLLGINNQPLLVMFKKA 208

[Close]

BLAST of Spo10773.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QKP3_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_168350 PE=4 SV=1)

HSP 1 Score: 428.3 bits (1100), Expect = 5.200e-117
Identity = 207/208 (99.52%), Postives = 207/208 (99.52%), Query Frame = 1


		  
Query: 1   MTNFILSAATFLFFLLSSPPSTTAAAKITPVFDINGRPLQTGSTYYILPVIRGRGGGLTM 60
           MTNFILSAATFLFFLLSSPPSTTAAAKITPVFDINGRPLQTGSTYYILPVIRGRGGGLTM
Sbjct: 1   MTNFILSAATFLFFLLSSPPSTTAAAKITPVFDINGRPLQTGSTYYILPVIRGRGGGLTM 60

Query: 61  TPINTTQSCPLYVAQENHEVSNGLPLKIYPVNPNEKRVPLGGDVNIVFDAASICVQSTGW 120
           TPINTTQSCPLYVAQENHEVSNGLPLKIYPVNPNEKRVPLGGDVNIVFDAASICVQSTGW
Sbjct: 61  TPINTTQSCPLYVAQENHEVSNGLPLKIYPVNPNEKRVPLGGDVNIVFDAASICVQSTGW 120

Query: 121 MLAFDEATGKQHVATGGTIGNPGVDTLSNWFRIEKAGSGMYDYKVSFCPGVCIFCAVMCG 180
           MLAFDEATGKQHVATGGTIGNPGVDTLSNWFRIEKAGSGMYDYKVSFCPGVCIFC VMCG
Sbjct: 121 MLAFDEATGKQHVATGGTIGNPGVDTLSNWFRIEKAGSGMYDYKVSFCPGVCIFCTVMCG 180

Query: 181 DVGVFIGEDGTRFLGLTDRPLLVKFKKA 209
           DVGVFIGEDGTRFLGLTDRPLLVKFKKA
Sbjct: 181 DVGVFIGEDGTRFLGLTDRPLLVKFKKA 208

[Close]

BLAST of Spo10773.1 vs. UniProtKB/TrEMBL
Match: A0A0J8EV86_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_6g153980 PE=4 SV=1)

HSP 1 Score: 329.7 bits (844), Expect = 2.500e-87
Identity = 161/206 (78.16%), Postives = 175/206 (84.95%), Query Frame = 1


		  
Query: 5   ILSAATFLFFLLSSPPSTT-AAAKITPVFDINGRPLQTGSTYYILPVIRGRGGGLTMTPI 64
           ILS  T L FLL +P STT AAAK T V DINGRPL+T STYYILPV RGRGGGL M P 
Sbjct: 6   ILSILTILLFLLIAPLSTTTAAAKTTTVLDINGRPLKTDSTYYILPVSRGRGGGLAMAPK 65

Query: 65  NTTQSCPLYVAQENHEVSNGLPLKIYPVNPNEK-RVPLGGDVNIVFDAASICVQSTGWML 124
           N T+SCPLYVAQENHEVSNGLPLK +P NPN+  ++PLG DVNIVFDAA+ICVQ TGWML
Sbjct: 66  NATESCPLYVAQENHEVSNGLPLKFFPTNPNDHDKIPLGYDVNIVFDAATICVQPTGWML 125

Query: 125 AFDEATGKQHVATGGTIGNPGVDTLSNWFRIEKAGSGMYDYKVSFCPGVCIFCAVMCGDV 184
           AFDEA+G ++V  GGTIGNPGVDTLSNWF IEKAGSG+YDYK+ FCP VCIFC VMCGDV
Sbjct: 126 AFDEASGTRYVGIGGTIGNPGVDTLSNWFAIEKAGSGLYDYKIRFCPAVCIFCTVMCGDV 185

Query: 185 GVFIGEDGTRFLGLTDRPLLVKFKKA 209
           GVFIGE GTRFL LTDRPLLV+FKKA
Sbjct: 186 GVFIGEGGTRFLALTDRPLLVRFKKA 211

[Close]

BLAST of Spo10773.1 vs. UniProtKB/TrEMBL
Match: A0A0J8C0T9_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_6g153990 PE=4 SV=1)

HSP 1 Score: 293.5 bits (750), Expect = 2.000e-76
Identity = 140/208 (67.31%), Postives = 161/208 (77.40%), Query Frame = 1


		  
Query: 1   MTNFILSAATFLFFLLSSPPSTTAAAKITPVFDINGRPLQTGSTYYILPVIRGRGGGLTM 60
           MT+ I+SAAT   FL  SP   T+ A  T V DINGRPLQ G  YYILPVIRGRGGGLTM
Sbjct: 1   MTHVIISAATIFLFLYLSP--LTSTADNTAVLDINGRPLQAGYNYYILPVIRGRGGGLTM 60

Query: 61  TPINTTQSCPLYVAQENHEVSNGLPLKIYPVNPNEKRVPLGGDVNIVFDAASICVQSTGW 120
              N T+ CPLYVAQE+HEVSNGLPLK YPVNP +KR+ L  D+N VFDAA+ CVQSTGW
Sbjct: 61  ASKNATELCPLYVAQEDHEVSNGLPLKFYPVNPKDKRISLSSDLNFVFDAATTCVQSTGW 120

Query: 121 MLAFDEATGKQHVATGGTIGNPGVDTLSNWFRIEKAGSGMYDYKVSFCPGVCIFCAVMCG 180
            L  +  TG+++V TGG IGNPGV+T+ NWF+IEK GSG YDYK+ +CPGVC FC VMCG
Sbjct: 121 SLTIEMETGRRYVGTGGEIGNPGVETVDNWFKIEKDGSGKYDYKIVYCPGVCNFCKVMCG 180

Query: 181 DVGVFIGEDGTRFLGLTDRPLLVKFKKA 209
           DVGVFI +DG R LG +D+PLLV FKKA
Sbjct: 181 DVGVFIEKDGRRLLGFSDQPLLVMFKKA 206

[Close]

BLAST of Spo10773.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QYY3_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_125760 PE=4 SV=1)

HSP 1 Score: 282.0 bits (720), Expect = 6.100e-73
Identity = 132/208 (63.46%), Postives = 159/208 (76.44%), Query Frame = 1


		  
Query: 1   MTNFILSAATFLFFLLSSPPSTTAAAKITPVFDINGRPLQTGSTYYILPVIRGRGGGLTM 60
           M ++ LSAAT L FL  SPP+TTAA     V DING PL+  S YYILPV+RGRGGGLTM
Sbjct: 1   MIHYFLSAATILLFLSLSPPTTTAANPA--VLDINGHPLRPRSNYYILPVVRGRGGGLTM 60

Query: 61  TPINTTQSCPLYVAQENHEVSNGLPLKIYPVNPNEKRVPLGGDVNIVFDAASICVQSTGW 120
              NTT+ CPLYVAQENHEVSNGLPLK Y VNP + ++ +  D+N VFDA++ICVQS  W
Sbjct: 61  LSKNTTEVCPLYVAQENHEVSNGLPLKFYSVNPKDTKIFVSTDLNFVFDASTICVQSNTW 120

Query: 121 MLAFDEATGKQHVATGGTIGNPGVDTLSNWFRIEKAGSGMYDYKVSFCPGVCIFCAVMCG 180
            L  DE TG++++  GG IGNPGV T+SNWF+IEKAGSG YDYK+ +C GVC FC +MCG
Sbjct: 121 ELTIDEETGRRYIGVGGEIGNPGVQTVSNWFKIEKAGSGKYDYKIVYCAGVCNFCKIMCG 180

Query: 181 DVGVFIGEDGTRFLGLTDRPLLVKFKKA 209
           D+G F+ EDG R LG + +PLLV+FKKA
Sbjct: 181 DIGAFVEEDGRRLLGFSGQPLLVRFKKA 206

[Close]

BLAST of Spo10773.1 vs. UniProtKB/TrEMBL
Match: A0A0J8C4X4_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_6g154000 PE=4 SV=1)

HSP 1 Score: 275.8 bits (704), Expect = 4.400e-71
Identity = 135/205 (65.85%), Postives = 158/205 (77.07%), Query Frame = 1


		  
Query: 5   ILSAATFLFFLLSSPPSTTAAAKITPVFDINGRPLQTGSTYYILPVIRGRGGGLTMTPIN 64
           ILSAAT   FL  SP ++TAA     V DINGRPLQ  S YYILPVIRGRGGGL MTP N
Sbjct: 6   ILSAATIFLFLCLSPLTSTAAN--IAVLDINGRPLQARSNYYILPVIRGRGGGLRMTPKN 65

Query: 65  TTQSCPL-YVAQENHEVSNGLPLKIYPVNPNEKRVPLGGDVNIVFDAASICVQSTGWMLA 124
            TQ CPL YVAQE  E++NGLPLK YPVNP +K + L  D+N VFDAA+ICVQST W LA
Sbjct: 66  ATQLCPLAYVAQEGSELANGLPLKFYPVNPKDKTISLSTDLNFVFDAATICVQSTQWRLA 125

Query: 125 FDEATGKQHVATGGTIGNPGVDTLSNWFRIEKAGSGMYDYKVSFCPGVCIFCAVMCGDVG 184
           FDE TG+++V  GG IGNPG +T+SNWF+IEKA +G YDYK+ FCPGVC FC V CG++G
Sbjct: 126 FDEVTGRRYVGFGGEIGNPGGNTVSNWFKIEKAETGKYDYKIVFCPGVCNFCKVACGEIG 185

Query: 185 VFIGEDGTRFLGLTDRPLLVKFKKA 209
           VF+ +DG R LG+ ++PLLV FKKA
Sbjct: 186 VFVEKDGRRLLGINNQPLLVMFKKA 208

[Close]

BLAST of Spo10773.1 vs. ExPASy Swiss-Prot
Match: MIRA_SYNDU (Miraculin OS=Synsepalum dulcificum PE=1 SV=3)

HSP 1 Score: 162.5 bits (410), Expect = 4.800e-39
Identity = 94/210 (44.76%), Postives = 124/210 (59.05%), Query Frame = 1


		  
Query: 4   FILSAATFLFFLLSSPPSTTAAAKITPVFDINGRPLQTGSTYYILPVIRGRGGGLTM--T 63
           F +SA   L    ++P  + A +   PV DI+G  L+TG+ YYI+PV+R  GGGLT+  T
Sbjct: 12  FFVSA---LLAAAANPLLSAADSAPNPVLDIDGEKLRTGTNYYIVPVLRDHGGGLTVSAT 71

Query: 64  PINTTQSCPLYVAQENHEVSNGLPLKIYPVNPNEKRVPLGGDVNIVFDAASIC--VQSTG 123
             N T  CP  V Q   EV +  PL  +P NP E  V +  D+NI F A   C    ST 
Sbjct: 72  TPNGTFVCPPRVVQTRKEVDHDRPLAFFPENPKEDVVRVSTDLNINFSAFMPCRWTSSTV 131

Query: 124 WML-AFDEATGKQHVATGGTIGNPGVDTLSNWFRIEK-AGSGMYDYKVSFCPGVCIFCAV 183
           W L  +DE+TG+  V  GG  GNPG +T+S+WF+IE+  GSG Y  K+ FCP VC  C V
Sbjct: 132 WRLDKYDESTGQYFVTIGGVKGNPGPETISSWFKIEEFCGSGFY--KLVFCPTVCGSCKV 191

Query: 184 MCGDVGVFIGEDGTRFLGLTDRPLLVKFKK 208
            CGDVG++I + G R L L+D+P   +F K
Sbjct: 192 KCGDVGIYIDQKGRRRLALSDKPFAFEFNK 216

[Close]

BLAST of Spo10773.1 vs. ExPASy Swiss-Prot
Match: ASP_THECC (21 kDa seed protein OS=Theobroma cacao GN=ASP PE=2 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 9.100e-30
Identity = 77/188 (40.96%), Postives = 97/188 (51.60%), Query Frame = 1


		  
Query: 24  AAAKITPVFDINGRPLQTGSTYYILPVIRGRGGGLTMTPINTTQSCPLYVAQENHEVSNG 83
           A A  +PV D +G  LQTG  YY+L  I G GGG       T QSCP  V Q   ++ NG
Sbjct: 24  ANAANSPVLDTDGDELQTGVQYYVLSSISGAGGGGLALGRATGQSCPEIVVQRRSDLDNG 83

Query: 84  LPLKIYPVNPNEKRVPLGGDVNIVFDAA--SICVQSTGWML-AFDEATGKQHVATGGTIG 143
            P+     +  +  V +  DVNI F      +C  ST W L  +D + GK  V T G  G
Sbjct: 84  TPVIFSNADSKDDVVRVSTDVNIEFVPIRDRLCSTSTVWRLDNYDNSAGKWWVTTDGVKG 143

Query: 144 NPGVDTLSNWFRIEKAGSGMYDYKVSFCPGVCIFCAVMCGDVGVFIGEDGTRFLGLTDRP 203
            PG +TL +WF+IEKA  G+  YK  FCP VC  C  +C D+G    +DG   L L+D  
Sbjct: 144 EPGPNTLCSWFKIEKA--GVLGYKFRFCPSVCDSCTTLCSDIGRHSDDDGQIRLALSDNE 203

Query: 204 LLVKFKKA 209
               FKKA
Sbjct: 204 WAWMFKKA 209

[Close]

BLAST of Spo10773.1 vs. ExPASy Swiss-Prot
Match: IAAS_ORYSJ (Alpha-amylase/subtilisin inhibitor OS=Oryza sativa subsp. japonica GN=RASI PE=1 SV=2)

HSP 1 Score: 121.3 bits (303), Expect = 1.200e-26
Identity = 77/193 (39.90%), Postives = 100/193 (51.81%), Query Frame = 1


		  
Query: 21  STTAAAKITPVFDINGRPLQTGSTYYILPVIRGRGGGLTMTPINTTQSCPLYVAQENHEV 80
           S + +A   PV+D  G  L    +YY+LP   G GGGLTM P      CPL VAQE  E 
Sbjct: 17  SFSCSAAPPPVYDTEGHELSADGSYYVLPASPGHGGGLTMAP--RVLPCPLLVAQETDER 76

Query: 81  SNGLPLKIYP----VNPNEKRVPLGGDVNIVFDAASICVQSTGWMLAFDEATGKQHVATG 140
             G P++  P      P ++ + +  DV I F+AA+ICVQST W +  +  TG + V TG
Sbjct: 77  RKGFPVRFTPWGGAAAPEDRTIRVSTDVRIRFNAATICVQSTEWHVGDEPLTGARRVVTG 136

Query: 141 GTIGNPGVDTLSNWFRIEKAGSGMYDYKVSFCPGVCIFCAVMCGDVGVFIGEDGTR-FLG 200
             IG P      N FR+EK G G   YK+       + C   C D+GV    DG R +LG
Sbjct: 137 PLIG-PSPSGRENAFRVEKYGGG---YKL-------VSCRDSCQDLGV--SRDGARAWLG 194

Query: 201 LTDRPLLVKFKKA 209
            +  P +V FKKA
Sbjct: 197 ASQPPHVVVFKKA 194

[Close]

BLAST of Spo10773.1 vs. ExPASy Swiss-Prot
Match: IAAS_WHEAT (Endogenous alpha-amylase/subtilisin inhibitor OS=Triticum aestivum PE=1 SV=1)

HSP 1 Score: 120.6 bits (301), Expect = 2.100e-26
Identity = 73/183 (39.89%), Postives = 105/183 (57.38%), Query Frame = 1


		  
Query: 30  PVFDINGRPLQTGSTYYILPVIRGRGGGLTMTPINTTQSCPLYVAQENHEVSNGLPLKIY 89
           PV D +G  L+  + YY+LP  R  GGGLTM P    + CPL+V+QE     +GLP++I 
Sbjct: 4   PVHDTDGNELRADANYYVLPANRAHGGGLTMAP-GHGRRCPLFVSQEADGQRDGLPVRIA 63

Query: 90  PVN--PNEKRVPLGGDVNIVFDAASICVQSTGWMLAFDEATGKQHVATGGTIGNPGVDTL 149
           P    P++K + L  DV I F A + CVQST W +  +  +G++HV T G + +P     
Sbjct: 64  PHGGAPSDKIIRLSTDVRISFRAYTTCVQSTEWHIDSELVSGRRHVIT-GPVRDPSPSGR 123

Query: 150 SNWFRIEK-AGSGMYDYKVSFCPGVCIFCAVMCGDVGVFIG-EDGTRFLGLTDRPLLVKF 209
            N FRIEK +G+ +++YK+       + C   C D+GVF   + G  FLG T+   +V F
Sbjct: 124 ENAFRIEKYSGAEVHEYKL-------MACGDSCQDLGVFRDLKGGAWFLGATEPYHVVVF 177

[Close]

BLAST of Spo10773.1 vs. ExPASy Swiss-Prot
Match: IAAS_HORVU (Alpha-amylase/subtilisin inhibitor OS=Hordeum vulgare PE=1 SV=2)

HSP 1 Score: 119.8 bits (299), Expect = 3.600e-26
Identity = 76/195 (38.97%), Postives = 111/195 (56.92%), Query Frame = 1


		  
Query: 18  SPPSTTAAAKITPVFDINGRPLQTGSTYYILPVIRGRGGGLTMTPINTTQSCPLYVAQEN 77
           +PPS   AA   PV D +G  L+  + YY+L   R  GGGLTM P    + CPL+V+Q+ 
Sbjct: 17  APPSR--AADPPPVHDTDGHELRADANYYVLSANRAHGGGLTMAP-GHGRHCPLFVSQDP 76

Query: 78  HEVSNGLPLKIYP--VNPNEKRVPLGGDVNIVFDAASICVQSTGWMLAFDEATGKQHVAT 137
           +   +G P++I P  V P++K + L  DV I F A + C+QST W +  + A G++HV T
Sbjct: 77  NGQHDGFPVRITPYGVAPSDKIIRLSTDVRISFRAYTTCLQSTEWHIDSELAAGRRHVIT 136

Query: 138 GGTIGNPGVDTLSNWFRIEK-AGSGMYDYKVSFCPGVCIFCAVMCGDVGVFIG-EDGTRF 197
            G + +P      N FRIEK +G+ +++YK+       + C   C D+GVF   + G  F
Sbjct: 137 -GPVKDPSPSGRENAFRIEKYSGAEVHEYKL-------MSCGDWCQDLGVFRDLKGGAWF 196

Query: 198 LGLTDRPLLVKFKKA 209
           LG T+   +V FKKA
Sbjct: 197 LGATEPYHVVVFKKA 200

[Close]

BLAST of Spo10773.1 vs. TAIR (Arabidopsis)
Match: AT1G17860.1 (Kunitz family trypsin and protease inhibitor protein)

HSP 1 Score: 177.2 bits (448), Expect = 1.100e-44
Identity = 101/207 (48.79%), Postives = 130/207 (62.80%), Query Frame = 1


		  
Query: 6   LSAATFLFFLLS---SPPSTTAAAKITPVFDINGRPLQTGSTYYILPVIRGRGGGLTMTP 65
           +S+  ++F LL+   S    T  A + PV DING+ L TG  YYILPVIRGRGGGLTM+ 
Sbjct: 1   MSSLLYIFLLLAVFISHRGVTTEAAVEPVKDINGKSLLTGVNYYILPVIRGRGGGLTMSN 60

Query: 66  INTTQSCPLYVAQENHEVSNGLPLKIYPVNPNEKRVPLGGDVNIVFDAASICVQSTGWML 125
           +  T++CP  V Q+  EVS GLP+K  P +   + +P+  DVNI F   SI      W L
Sbjct: 61  LK-TETCPTSVIQDQFEVSQGLPVKFSPYD-KSRTIPVSTDVNIKFSPTSI------WEL 120

Query: 126 A-FDEATGKQHVATGGTIGNPGVDTLSNWFRIEKAGSGMYDYKVSFCPGVCIFCAVMCGD 185
           A FDE T +  ++T G  GNPG  T+ NWF+I+K      DYK+ FCP VC FC V+C D
Sbjct: 121 ANFDETTKQWFISTCGVEGNPGQKTVDNWFKIDKFEK---DYKIRFCPTVCNFCKVICRD 180

Query: 186 VGVFIGEDGTRFLGLTDRPLLVKFKKA 209
           VGVF+ +DG R L L+D PL V FK+A
Sbjct: 181 VGVFV-QDGKRRLALSDVPLKVMFKRA 195

[Close]

BLAST of Spo10773.1 vs. TAIR (Arabidopsis)
Match: AT1G73260.1 (kunitz trypsin inhibitor 1)

HSP 1 Score: 128.3 bits (321), Expect = 5.700e-30
Identity = 81/198 (40.91%), Postives = 108/198 (54.55%), Query Frame = 1


		  
Query: 13  FFLLSSPPSTTAAAKITPVFDINGRPLQTGSTYYILPVIRGRGGGLTMTPINTTQSCPLY 72
           F+L+ +  +  A+     V DI+G  +   S YY+LPVIRGRGGGLT+      Q CP  
Sbjct: 12  FYLVLALTAVLASNAYGAVVDIDGNAMFHES-YYVLPVIRGRGGGLTLAG-RGGQPCPYD 71

Query: 73  VAQENHEVSNGLPLKIYPVNPNEKRVPLGGDVNIVFDA-ASICVQSTGWMLA-FDEATGK 132
           + QE+ EV  G+P+K          VP   ++NI  D  A+IC+QST W +  FD    +
Sbjct: 72  IVQESSEVDEGIPVKFSNWRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHERKQ 131

Query: 133 QHVATGGTIGNPGVDTLSNWFRIEKAGSGMYDYKVSFCPGVCIFCAVMCGDVGVFIGEDG 192
             V  G      G D+L ++F+IEK+G   Y  K  FCP  C      C DVG+FI E G
Sbjct: 132 YFVVAGPKPEGFGQDSLKSFFKIEKSGEDAY--KFVFCPRTCDSGNPKCSDVGIFIDELG 191

Query: 193 TRFLGLTDRPLLVKFKKA 209
            R L L+D+P LV FKKA
Sbjct: 192 VRRLALSDKPFLVMFKKA 205

The following BLAST results are available for this feature:

BLAST of Spo10773.1 vs. NCBI nr
BLAST of Spo10773.1 vs. UniProtKB/TrEMBL
BLAST of Spo10773.1 vs. ExPASy Swiss-Prot
BLAST of Spo10773.1 vs. TAIR (Arabidopsis)

BLAST of Spo10773.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902170260\|gb\|KNA07819.1\|	7.5e-117	99.5	hypothetical protein SOVF_1683...	[more]
gi\|731344029\|ref\|XP_010683192.1\|	3.6e-87	78.1	PREDICTED: miraculin-like [Bet...	[more]
gi\|731344031\|ref\|XP_010683193.1\|	2.9e-76	67.3	PREDICTED: miraculin-like [Bet...	[more]
gi\|902194163\|gb\|KNA12470.1\|	8.7e-73	63.4	hypothetical protein SOVF_1257...	[more]
gi\|731344033\|ref\|XP_010683194.1\|	6.2e-71	65.8	PREDICTED: miraculin-like [Bet...	[more]

BLAST of Spo10773.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9QKP3_SPIOL	5.2e-117	99.5	Uncharacterized protein OS=Spi...	[more]
A0A0J8EV86_BETVU	2.5e-87	78.1	Uncharacterized protein OS=Bet...	[more]
A0A0J8C0T9_BETVU	2.0e-76	67.3	Uncharacterized protein OS=Bet...	[more]
A0A0K9QYY3_SPIOL	6.1e-73	63.4	Uncharacterized protein OS=Spi...	[more]
A0A0J8C4X4_BETVU	4.4e-71	65.8	Uncharacterized protein OS=Bet...	[more]

BLAST of Spo10773.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
MIRA_SYNDU	4.8e-39	44.7	Miraculin OS=Synsepalum dulcif...	[more]
ASP_THECC	9.1e-30	40.9	21 kDa seed protein OS=Theobro...	[more]
IAAS_ORYSJ	1.2e-26	39.9	Alpha-amylase/subtilisin inhib...	[more]
IAAS_WHEAT	2.1e-26	39.8	Endogenous alpha-amylase/subti...	[more]
IAAS_HORVU	3.6e-26	38.9	Alpha-amylase/subtilisin inhib...	[more]

BLAST of Spo10773.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 2


Match Name	E-value	Identity	Description
AT1G17860.1	1.1e-44	48.7	Kunitz family trypsin and prot...	[more]
AT1G73260.1	5.7e-30	40.9	kunitz trypsin inhibitor 1	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002160	Proteinase inhibitor I3, Kunitz legume	PRINTS	PR00291	KUNITZINHBTR	coord: 150..169 score: 2.1E-21coord: 30..59 score: 2.1E-21coord: 69..89 score: 2.1
IPR002160	Proteinase inhibitor I3, Kunitz legume	PFAM	PF00197	Kunitz_legume	coord: 31..207 score: 1.0
IPR002160	Proteinase inhibitor I3, Kunitz legume	SMART	SM00452	kul_2	coord: 30..208 score: 3.7
IPR002160	Proteinase inhibitor I3, Kunitz legume	PROSITE	PS00283	SOYBEAN_KUNITZ	coord: 31..47 scor
IPR011065	Kunitz inhibitor ST1-like	unknown	SSF50386	STI-like	coord: 29..208 score: 1.64
None	No IPR available	GENE3D	2.80.10.50		coord: 29..208 score: 3.3
None	No IPR available	PANTHER	PTHR33107	FAMILY NOT NAMED	coord: 1..208 score: 5.8

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0004866	endopeptidase inhibitor activity