Spo11269.1 (mRNA) | SpinachBase

Overview


Name	Spo11269.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	Pentatricopeptide repeat-containing protein, putative
Location	SpoScf_02677 : 42485 .. 45136 (-)
Sequence length	304

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTGCCGAAAACGGAAACATGGTACGTATCGGAAAATATTATCGGAAATGGAAATATTGCCGGAATCGGAAATATTGCCGGAAACGGAAATATTGTCAGAATCGGAAATATTATCGGAATCGGAAAATAATTCCGGAAACGGAAATATTAAATATTTGTTCGAAACGGAAATTAATTCCGGAATCGGAAATGTTAAATATTGTTCGTATCAGAAATGAAATCCGGAATCGGAAATTTAATTGGAAGCGTATCGTACGAATTAGCATCGGACGAGACTTGCTAGACGAAGGCCCAGCACGAAGCCAGGCCGTCGCCCAGCAAGCCACACGCATCAACACACGCCAAAGTCAGCCAGGGCCAGCGCAAGGCCAGGCCTAGCAAGGCATGGCGCGCGCTCTCAACAAATGGGTTGCGAGCATTGGGCCATGGCGCCGTGCAGCTCGCGTGGGCCGCGAGGCATGCGCGTGGGCTGTGCGTTGCTCGTGGTCGTGCTCGTGCTCGTGTGCATGCTATACGAATCCTAAAGCTATCGGAATTCGTGCTATGATTAAATCCTAATCCTAAAAGATAAAATTAATTATTTAGAGTTTTAAAAGGATTCTAATTAATTAATTAGTATTCTAACAGGATTCGAAATCCTTTTCCATGGTTCTATAAATATATGCCTAGGGTCATAAATTTATACAAGAGTTTTTAACAAGTATTCATACAATAAAAGCTGTGATTTTTGAGCAGAAAAATTAGTCACATTACTTGCCCATTTAGCCGAAAATAATAGTACCTTAAGGGCGATTCTAGTTGGTCAATCTTAAGGCGGATCCGGACGTGCTGTGGACTATCTACGGAGGGACGACACTTGGAGTCCTAAAGACTTGTTCGTGTTCGGTTCGGGCGCAGCTAGGGAGAGCACGCTACAAAGTGTATGCATCTAAATTATGCTAATTGATTATGTGTAAATAATATGTTTCCTGGCATTAAGGTTTTTCCGCATGATTTATGTTTTTCATATGTATCATAACCTAACACTATCAACCTTGGTACCCTACATATTTTTCTCCTAAAAACATTACTCACACTTTCTTTTTCATTTCACCATATTTAATAGATTCGTTTTTTATTGTATTGAATTTTATATTAGTACTTTGAAGCAATTGAAGTATATTGCAATCATGCTTAAAATTTCATTAGTCATATTTATCTATTTAAAAAATATATATATTATTAAAAAAATAATATAAAGAACAATTAAAAAAGAAAAAAATTATTTAGTCAATGTTTATTAGAAAAATATAGAGAGAAAAAATTAACACAATAAAATATTTGTCTAATATTAATAAAGAAATAAAATGTGTCAATGTCCTTAATGGTCATTTTACATATTAAAAAGCTAACAGCTATCAGCTAATTTACCAAACAGTTTTACACAAACAGTTAATTCAACAAGCTAGTCAAATCCGTTAATACAAACAACTAATAGCTAACACCTAGTCAAACCAGCTAACAACTCCTAAACAAACAGGGCCTTAGATTTTGGAACTCACCTTCACACTTGTCGTAGTGTTTTATACTTCCTCTATTCTTATTTATATGACATAATTGTGGTTTTGACACTATTCACACAAGCTCCTTTGATTTCAATTTGTGATTTATTTTTAAGAAAAAACATAGTTGTGTGGGATCTTGTTTGATTCGTCTTAATAAATATTTTTTAAATATCAACTTTTATATTTTTTTTCTTATCCATAATTGAAGATATTAAAGTTTGAAATCATGCATTGACAAGCGTGACTAAATAATTGTGTCATATAAATAAGAACAGAGGAAGTAACATTTATCTCAAATTCTCAATAGTCTAGCCAGTGTAGGCACAAACTTTCTTCAAGACTCCTCTCAGACTCGGAGTGGGGATAATCTCACCCTTCGTCATTGATCTATCTTAAACAGCCCGAGCTCCAACCGGCCGATCCCCTTGATTGGCGAGTAAACATATCAAGGGGTATTTTCGTAAATAAATTATCAAAATATACAATCTACATATTTGAGTATACAATATCTTTTTGTATTAATTTTTTTTTTTTTATAATATCAAATATGTTGTACATTGCAATTTCTAAATTATATATTTGCTAATTGTAATTACAAAAATACCACTTGATATGTCAATCAAGGGATTGTTTTGAAATTTCTCTAAACAGCCCACCTGAAAGAAAGACAGTCTACTTTAGTCTAGTGTGTAGTACTGCTACTGACGCTGCGTTCTCATCGCTACCATTTCTTAAATTCCAACCTCAAAAATCCTGAACTGAAGTTCAAAATAGCTCCCAGTAACGGCAATTATACTCCATTCAATCCAACACTCAAACAAGCAGATTTGCAAGAATTCCCAATAAAACACATCAAATTCATCAATTTACTCCACAAAACAGAGTTAAACTAACAAGTGATGATTTCAATGCAAACCTTATCCCTACCACCTTCACCCACACCAAACCCACCACCATTTTCTCTCTCCAAACCTTCCTTCTCACAGAGAGTCTACATCCCTTCCTATATTTACAAACACCCATCTGCAATTCTCCTCGAAATCTGCACCTCAATTCAAGAACTCCGTCAAATCGTTCCTATTATTTACAAACATGGATTATACGAT

mRNA sequence

ATGTTGCCGAAAACGGAAACATGATTTGCAAGAATTCCCAATAAAACACATCAAATTCATCAATTTACTCCACAAAACAGAGTTAAACTAACAAGTGATGATTTCAATGCAAACCTTATCCCTACCACCTTCACCCACACCAAACCCACCACCATTTTCTCTCTCCAAACCTTCCTTCTCACAGAGAGTCTACATCCCTTCCTATATTTACAAACACCCATCTGCAATTCTCCTCGAAATCTGCACCTCAATTCAAGAACTCCGTCAAATCGTTCCTATTATTTACAAACATGGATTATACGAT

Coding sequence (CDS)

ATGATTTCAATGCAAACCTTATCCCTACCACCTTCACCCACACCAAACCCACCACCATTTTCTCTCTCCAAACCTTCCTTCTCACAGAGAGTCTACATCCCTTCCTATATTTACAAACACCCATCTGCAATTCTCCTCGAAATCTGCACCTCAATTCAAGAACTCCGTCAAATCGTTCCTATTATTTACAAACATGGATTATACGAT

Protein sequence

MISMQTLSLPPSPTPNPPPFSLSKPSFSQRVYIPSYIYKHPSAILLEICTSIQELRQIVPIIYKHGLYD

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo11269	Spo11269	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo11269.1	Spo11269.1-protein	polypeptide

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo11269.1.utr5p.2	Spo11269.1.utr5p.2	five_prime_UTR
Spo11269.1.utr5p.1	Spo11269.1.utr5p.1	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo11269.1.CDS.1	Spo11269.1.CDS.1	CDS

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo11269.1.exon.2	Spo11269.1.exon.2	exon
Spo11269.1.exon.1	Spo11269.1.exon.1	exon

Homology

[Close]

BLAST of Spo11269.1 vs. NCBI nr
Match: gi|902208628|gb|KNA15996.1| (hypothetical protein SOVF_093190 [Spinacia oleracea])

HSP 1 Score: 140.2 bits (352), Expect = 1.400e-30
Identity = 69/69 (100.00%), Postives = 69/69 (100.00%), Query Frame = 1


		  
Query: 1  MISMQTLSLPPSPTPNPPPFSLSKPSFSQRVYIPSYIYKHPSAILLEICTSIQELRQIVP 60
          MISMQTLSLPPSPTPNPPPFSLSKPSFSQRVYIPSYIYKHPSAILLEICTSIQELRQIVP
Sbjct: 1  MISMQTLSLPPSPTPNPPPFSLSKPSFSQRVYIPSYIYKHPSAILLEICTSIQELRQIVP 60

Query: 61 IIYKHGLYD 70
          IIYKHGLYD
Sbjct: 61 IIYKHGLYD 69

[Close]

BLAST of Spo11269.1 vs. NCBI nr
Match: gi|731365648|ref|XP_010694640.1| (PREDICTED: pentatricopeptide repeat-containing protein At1g11290 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 92.4 bits (228), Expect = 3.200e-16
Identity = 49/68 (72.06%), Postives = 55/68 (80.88%), Query Frame = 1


		  
Query: 2  ISMQTLSLPPSPTPNPPP-FSLSKPSFSQRVYIPSYIYKHPSAILLEICTSIQELRQIVP 61
          ISMQTLSLP S T  P    SLS+P  SQRVYIPS+IYKHP+AILLE+CTSI+EL QI+P
Sbjct: 4  ISMQTLSLPSSSTITPSSQLSLSRPPLSQRVYIPSHIYKHPTAILLELCTSIKELHQIIP 63

Query: 62 IIYKHGLY 69
           IYKH LY
Sbjct: 64 HIYKHNLY 71

[Close]

BLAST of Spo11269.1 vs. NCBI nr
Match: gi|694366641|ref|XP_009361929.1| (PREDICTED: pentatricopeptide repeat-containing protein At1g11290 [Pyrus x bretschneideri])

HSP 1 Score: 76.6 bits (187), Expect = 1.800e-11
Identity = 40/70 (57.14%), Postives = 52/70 (74.29%), Query Frame = 1


		  
Query: 6  TLSLPPSPTPNP--PPFSLSKP----SFSQRVYIPSYIYKHPSAILLEICTSIQELRQIV 65
          T  LPP PTP+   PP S  K     + SQR +IPS++YKHP+AILLE+CTS+QEL QI+
Sbjct: 19 TPPLPPPPTPSRLRPPVSTPKSQTFHTLSQRTHIPSHVYKHPAAILLELCTSVQELNQII 78

Query: 66 PIIYKHGLYD 70
          P+I K+GLY+
Sbjct: 79 PLIIKNGLYN 88

[Close]

BLAST of Spo11269.1 vs. NCBI nr
Match: gi|298205132|emb|CBI40653.3| (unnamed protein product [Vitis vinifera])

HSP 1 Score: 74.3 bits (181), Expect = 9.200e-11
Identity = 38/67 (56.72%), Postives = 47/67 (70.15%), Query Frame = 1


		  
Query: 2   ISMQTLSLPPSPTPNPPPFSLSKPSFSQRVYIPSYIYKHPSAILLEICTSIQELRQIVPI 61
           I   TL LPP P P+P       PS   R YIPS++YKHPSAILLE+CTS++EL Q +P+
Sbjct: 48  IPTNTLPLPPPPPPSPTSNLHRTPS--SRTYIPSHVYKHPSAILLELCTSMKELHQFIPL 107

Query: 62  IYKHGLY 69
           I K+GLY
Sbjct: 108 IIKNGLY 112

[Close]

BLAST of Spo11269.1 vs. NCBI nr
Match: gi|658042344|ref|XP_008356785.1| (PREDICTED: pentatricopeptide repeat-containing protein At1g11290-like [Malus domestica])

HSP 1 Score: 74.3 bits (181), Expect = 9.200e-11
Identity = 39/72 (54.17%), Postives = 50/72 (69.44%), Query Frame = 1


		  
Query: 6  TLSLPPSPTPNP----PPFSLSKP----SFSQRVYIPSYIYKHPSAILLEICTSIQELRQ 65
          T  LPP P P P    PP +  K     + SQR +IPS++YKHP+AILLE+CTS QEL Q
Sbjct: 19 TPPLPPPPPPTPSRLRPPIATPKSQTFHTLSQRTHIPSHVYKHPAAILLELCTSXQELNQ 78

Query: 66 IVPIIYKHGLYD 70
          I+P+I K+GLY+
Sbjct: 79 IIPLIIKNGLYN 90

[Close]

BLAST of Spo11269.1 vs. UniProtKB/TrEMBL
Match: A0A0K9R922_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_093190 PE=4 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 9.500e-31
Identity = 69/69 (100.00%), Postives = 69/69 (100.00%), Query Frame = 1


		  
Query: 1  MISMQTLSLPPSPTPNPPPFSLSKPSFSQRVYIPSYIYKHPSAILLEICTSIQELRQIVP 60
          MISMQTLSLPPSPTPNPPPFSLSKPSFSQRVYIPSYIYKHPSAILLEICTSIQELRQIVP
Sbjct: 1  MISMQTLSLPPSPTPNPPPFSLSKPSFSQRVYIPSYIYKHPSAILLEICTSIQELRQIVP 60

Query: 61 IIYKHGLYD 70
          IIYKHGLYD
Sbjct: 61 IIYKHGLYD 69

[Close]

BLAST of Spo11269.1 vs. UniProtKB/TrEMBL
Match: A0A0J8E4W7_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_4g094640 PE=4 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 2.300e-16
Identity = 49/68 (72.06%), Postives = 55/68 (80.88%), Query Frame = 1


		  
Query: 2  ISMQTLSLPPSPTPNPPP-FSLSKPSFSQRVYIPSYIYKHPSAILLEICTSIQELRQIVP 61
          ISMQTLSLP S T  P    SLS+P  SQRVYIPS+IYKHP+AILLE+CTSI+EL QI+P
Sbjct: 4  ISMQTLSLPSSSTITPSSQLSLSRPPLSQRVYIPSHIYKHPTAILLELCTSIKELHQIIP 63

Query: 62 IIYKHGLY 69
           IYKH LY
Sbjct: 64 HIYKHNLY 71

[Close]

BLAST of Spo11269.1 vs. UniProtKB/TrEMBL
Match: F6I6N4_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_13s0067g02100 PE=4 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 6.400e-11
Identity = 38/67 (56.72%), Postives = 47/67 (70.15%), Query Frame = 1


		  
Query: 2   ISMQTLSLPPSPTPNPPPFSLSKPSFSQRVYIPSYIYKHPSAILLEICTSIQELRQIVPI 61
           I   TL LPP P P+P       PS   R YIPS++YKHPSAILLE+CTS++EL Q +P+
Sbjct: 48  IPTNTLPLPPPPPPSPTSNLHRTPS--SRTYIPSHVYKHPSAILLELCTSMKELHQFIPL 107

Query: 62  IYKHGLY 69
           I K+GLY
Sbjct: 108 IIKNGLY 112

[Close]

BLAST of Spo11269.1 vs. UniProtKB/TrEMBL
Match: A0A067L904_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_24583 PE=4 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 1.100e-10
Identity = 37/73 (50.68%), Postives = 52/73 (71.23%), Query Frame = 1


		  
Query: 2  ISMQTLSLPPSPTPNPPPF-----SLSKPSFSQRVYIPSYIYKHPSAILLEICTSIQELR 61
          +S Q L    +PTP   P      S + P+ S+R++IP+YIYKHP+AILLE+CTSI+EL 
Sbjct: 1  MSSQLLPFASTPTPPSSPLYSKTRSSASPTLSKRIHIPAYIYKHPAAILLELCTSIKELH 60

Query: 62 QIVPIIYKHGLYD 70
          QI+P+I K+G Y+
Sbjct: 61 QILPLIIKNGFYN 73

[Close]

BLAST of Spo11269.1 vs. UniProtKB/TrEMBL
Match: W9R9T7_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_012217 PE=4 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 2.400e-10
Identity = 41/72 (56.94%), Postives = 52/72 (72.22%), Query Frame = 1


		  
Query: 1  MISMQTLSLPPSPTPNPPPFSLSKP----SFSQRVYIPSYIYKHPSAILLEICTSIQELR 60
          MIS Q L + PSP P     S S P    + SQR +IPS++YKHP+AILLE+ TS+QELR
Sbjct: 1  MISSQVLPIAPSP-PTKLRLSTSLPGTFHTLSQRTHIPSHVYKHPAAILLELSTSLQELR 60

Query: 61 QIVPIIYKHGLY 69
          QI+P+I K+GLY
Sbjct: 61 QILPLIIKNGLY 71

[Close]

BLAST of Spo11269.1 vs. ExPASy Swiss-Prot
Match: PPR32_ARATH (Pentatricopeptide repeat-containing protein At1g11290, chloroplastic OS=Arabidopsis thaliana GN=PCMP-H40 PE=2 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 4.500e-10
Identity = 29/54 (53.70%), Postives = 44/54 (81.48%), Query Frame = 1


		  
Query: 15 PNPPPFSLSKPSFSQRVYIPSYIYKHPSAILLEICTSIQELRQIVPIIYKHGLY 69
          PNPP  S  +   S+R YIP+ +Y+HP+A+LLE C+S++ELRQI+P+++K+GLY
Sbjct: 15 PNPP--SRHRHFLSERNYIPANVYEHPAALLLERCSSLKELRQILPLVFKNGLY 66

[Close]

BLAST of Spo11269.1 vs. TAIR (Arabidopsis)
Match: AT1G11290.1 (Pentatricopeptide repeat (PPR) superfamily protein)

HSP 1 Score: 64.7 bits (156), Expect = 2.600e-11
Identity = 29/54 (53.70%), Postives = 44/54 (81.48%), Query Frame = 1


		  
Query: 15 PNPPPFSLSKPSFSQRVYIPSYIYKHPSAILLEICTSIQELRQIVPIIYKHGLY 69
          PNPP  S  +   S+R YIP+ +Y+HP+A+LLE C+S++ELRQI+P+++K+GLY
Sbjct: 15 PNPP--SRHRHFLSERNYIPANVYEHPAALLLERCSSLKELRQILPLVFKNGLY 66

The following BLAST results are available for this feature:

BLAST of Spo11269.1 vs. NCBI nr
BLAST of Spo11269.1 vs. UniProtKB/TrEMBL
BLAST of Spo11269.1 vs. ExPASy Swiss-Prot
BLAST of Spo11269.1 vs. TAIR (Arabidopsis)

BLAST of Spo11269.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902208628\|gb\|KNA15996.1\|	1.4e-30	100.	hypothetical protein SOVF_0931...	[more]
gi\|731365648\|ref\|XP_010694640.1\|	3.2e-16	72.0	PREDICTED: pentatricopeptide r...	[more]
gi\|694366641\|ref\|XP_009361929.1\|	1.8e-11	57.1	PREDICTED: pentatricopeptide r...	[more]
gi\|298205132\|emb\|CBI40653.3\|	9.2e-11	56.7	unnamed protein product [Vitis...	[more]
gi\|658042344\|ref\|XP_008356785.1\|	9.2e-11	54.1	PREDICTED: pentatricopeptide r...	[more]

BLAST of Spo11269.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9R922_SPIOL	9.5e-31	100.	Uncharacterized protein OS=Spi...	[more]
A0A0J8E4W7_BETVU	2.3e-16	72.0	Uncharacterized protein OS=Bet...	[more]
F6I6N4_VITVI	6.4e-11	56.7	Putative uncharacterized prote...	[more]
A0A067L904_JATCU	1.1e-10	50.6	Uncharacterized protein OS=Jat...	[more]
W9R9T7_9ROSA	2.4e-10	56.9	Uncharacterized protein OS=Mor...	[more]

BLAST of Spo11269.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 1


Match Name	E-value	Identity	Description
PPR32_ARATH	4.5e-10	53.7	Pentatricopeptide repeat-conta...	[more]

BLAST of Spo11269.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 1


Match Name	E-value	Identity	Description
AT1G11290.1	2.6e-11	53.7	Pentatricopeptide repeat (PPR)...	[more]

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016556	mRNA modification
biological_process	GO:0090305	nucleic acid phosphodiester bond hydrolysis
cellular_component	GO:0009507	chloroplast
molecular_function	GO:0004519	endonuclease activity