Spo12227.1 (mRNA) | SpinachBase

Overview


Name	Spo12227.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	Xyloglucan endotransglucosylase/hydrolase (2.4.1.207)
Location	SpoScf_01968 : 64845 .. 66861 (-)
Sequence length	835

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CTAAAACAAACAAAATTACTAAAAATAGATTCATGTACCACTTCATTACACTCCACTCCACTCTACTAAACAACACAAACTCACTCAAAATGAATAAATAAAAATAAAAAATAAATAAATAATACCCCATTTAAAAGGGTATTGTAGTATATTTACTCACCATAGCTGGCACCAGAAATGGCTACAGCTGGTGCCCTTAAGCTCCCCACTTCATTACACCATACCTTACACGCTCTTCCAAGGATTCCTTTACCCTTTATATACCCCCCATTACAACTCAAATTTATCATAACAATTCAAAAAATAAATTTATAAAAATAACAAATAATTTTGAAAAAGGAAATTAAAATATAAAATAATGGGTGTTTCTTCAAATGGGGTTTTAGTGATCATAGTAATGGTGGTTGCACTAGCATTGAGCACCATTACTAGTGTTAATGGTGGTGGTAATTTTGAACAAGATTTTGAGGTTACATTTGGTGATGGTAGGGTTAAAAAGTTTTGGAGAGGACAACTTCTAACCCTTTCTCTTGATAAGGTTTCGGGTTCGGGTTTTCGGTCTAAGAGGGAGTATTTGTTTGGAAGGATTGATATGCAACTTAAACTCGTTGCGGGTAATTCTGCTGGCACTGTCACTGCTTATTATGTAAGCCCCTTCTTTTCGAGCATTAACCTTTATTTTTTTACTAATGTCACTAACTCACTATAACAACTAAGTTTAATTGGATTGGACTACTTCGTAGTTTAAGAGACACTTCAATTCCTCTGTTCTATATAGTCGTGTTAGTGGGGTAATTTGAACTTGTGCAATAAAAATAAAAAAAGATGGAATTTTATGAGGAGTGCCAAAAACTATATGAATTATTAAACATCTCTTGTATATTCTGAGAAAAGATTATTTTTTCAAACAAGTAGTAATTTTGTTTTAAACATAATGTTTTTTATTCTTTTCTTTAAAATATGGCTTTATATTCTTCATTATATAAAATAAATGACCACCTTTTATCCAAATAATACTATTTTTTTTAAAAGGACTAGACTAAACAAACATCAACTCCATGTCAAAGTAATGACTTTAAATAATGTAGATCATATATACGGACTTACGGAGTAAATTAATTACAAAATCTAGCATACTAGTCCTGTAGTCCACGTCGAATATTATCCGTTCTTAAAAATTTGTCCCATACTCGTATTACAAGTAGTATTAGTTTACATTTACTTTCTTTGTTTCTAAATATTTAGTATTATACTTGTTAAAATATTAGTTTGACTAGAATTATATGCAAAAATATATTAATAAAAACCAAAAAAAATGATATTTGAAAAATTTAACAGACGAATTGAACAACAAATTTACACGATAATATTTAATTTTGATATATTTGTAATAAAATTTGATTAAAGATAAATATATAAATAGTGCAAAAGTGTTGTAATACAAAGGAATTAGAGAGTGATATATAGTGGTCGGATCACCGAAGTGGAGTGATGATGGATCGACCATGTGAAATTTATGTCTGGAATCATCTCTTATTTTTTCTTTTACGAATTGAAGGGTGGTGGTTAGGCTTATAACTTTAGCACAATTGCACGAGAAATAGAATATGTTATCTACTTTGGTTTTGGACTTATTTATTAGTAGTAGTAGTAGTACCAATAAATAATAATTGCTTTTTTTGTCAACTCATATCACTATTTTTGATTCTACATAGTAAATTAAGCATTCTTTTTTAAAAGAATAAACCCAAAGCATAATAAAGCACTCATTCCATTTTAAGATATCCTGAAAATAATTGTGACATTTTGTGTTACGTTGTATATGCAGTTATCTTCAGAAGGGAAGACACATGACGAGATAGATTTCGAGTTCTTAGGTAACGTAAGTGGGCAGCCTTACACACTTCACACTAATGTGTTTACCAAAGGGAAAGGTGATAGGGAGCAACAATTCCATCTTTGGTTCGACCCAACCTTAAAATTCCACACCTATTCTGTTGCGTGGACGCCCGTATGA

mRNA sequence

CTAAAACAAACAAAATTACTAAAAATAGATTCATGTACCACTTCATTACACTCCACTCCACTCTACTAAACAACACAAACTCACTCAAAATGAATAAATAAAAATAAAAAATAAATAAATAATACCCCATTTAAAAGGGTATTGTAGTATATTTACTCACCATAGCTGGCACCAGAAATGGCTACAGCTGGTGCCCTTAAGCTCCCCACTTCATTACACCATACCTTACACGCTCTTCCAAGGATTCCTTTACCCTTTATATACCCCCCATTACAACTCAAATTTATCATAACAATTCAAAAAATAAATTTATAAAAATAACAAATAATTTTGAAAAAGGAAATTAAAATATAAAATAATGGGTGTTTCTTCAAATGGGGTTTTAGTGATCATAGTAATGGTGGTTGCACTAGCATTGAGCACCATTACTAGTGTTAATGGTGGTGGTAATTTTGAACAAGATTTTGAGGTTACATTTGGTGATGGTAGGGTTAAAAAGTTTTGGAGAGGACAACTTCTAACCCTTTCTCTTGATAAGGTTTCGGGTTCGGGTTTTCGGTCTAAGAGGGAGTATTTGTTTGGAAGGATTGATATGCAACTTAAACTCGTTGCGGGTAATTCTGCTGGCACTGTCACTGCTTATTATTTATCTTCAGAAGGGAAGACACATGACGAGATAGATTTCGAGTTCTTAGGTAACGTAAGTGGGCAGCCTTACACACTTCACACTAATGTGTTTACCAAAGGGAAAGGTGATAGGGAGCAACAATTCCATCTTTGGTTCGACCCAACCTTAAAATTCCACACCTATTCTGTTGCGTGGACGCCCGTATGA

Coding sequence (CDS)

ATGGGTGTTTCTTCAAATGGGGTTTTAGTGATCATAGTAATGGTGGTTGCACTAGCATTGAGCACCATTACTAGTGTTAATGGTGGTGGTAATTTTGAACAAGATTTTGAGGTTACATTTGGTGATGGTAGGGTTAAAAAGTTTTGGAGAGGACAACTTCTAACCCTTTCTCTTGATAAGGTTTCGGGTTCGGGTTTTCGGTCTAAGAGGGAGTATTTGTTTGGAAGGATTGATATGCAACTTAAACTCGTTGCGGGTAATTCTGCTGGCACTGTCACTGCTTATTATTTATCTTCAGAAGGGAAGACACATGACGAGATAGATTTCGAGTTCTTAGGTAACGTAAGTGGGCAGCCTTACACACTTCACACTAATGTGTTTACCAAAGGGAAAGGTGATAGGGAGCAACAATTCCATCTTTGGTTCGACCCAACCTTAAAATTCCACACCTATTCTGTTGCGTGGACGCCCGTATGA

Protein sequence

MGVSSNGVLVIIVMVVALALSTITSVNGGGNFEQDFEVTFGDGRVKKFWRGQLLTLSLDKVSGSGFRSKREYLFGRIDMQLKLVAGNSAGTVTAYYLSSEGKTHDEIDFEFLGNVSGQPYTLHTNVFTKGKGDREQQFHLWFDPTLKFHTYSVAWTPV

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo12227	Spo12227	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo12227.1	Spo12227.1-protein	polypeptide

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo12227.1.utr5p.1	Spo12227.1.utr5p.1	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo12227.1.CDS.2	Spo12227.1.CDS.2	CDS
Spo12227.1.CDS.1	Spo12227.1.CDS.1	CDS

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo12227.1.exon.2	Spo12227.1.exon.2	exon
Spo12227.1.exon.1	Spo12227.1.exon.1	exon

Homology

[Close]

BLAST of Spo12227.1 vs. NCBI nr
Match: gi|902166498|gb|KNA07231.1| (hypothetical protein SOVF_173740 [Spinacia oleracea])

HSP 1 Score: 320.9 bits (821), Expect = 1.300e-84
Identity = 157/157 (100.00%), Postives = 157/157 (100.00%), Query Frame = 1


		  
Query: 1   MGVSSNGVLVIIVMVVALALSTITSVNGGGNFEQDFEVTFGDGRVKKFWRGQLLTLSLDK 60
           MGVSSNGVLVIIVMVVALALSTITSVNGGGNFEQDFEVTFGDGRVKKFWRGQLLTLSLDK
Sbjct: 1   MGVSSNGVLVIIVMVVALALSTITSVNGGGNFEQDFEVTFGDGRVKKFWRGQLLTLSLDK 60

Query: 61  VSGSGFRSKREYLFGRIDMQLKLVAGNSAGTVTAYYLSSEGKTHDEIDFEFLGNVSGQPY 120
           VSGSGFRSKREYLFGRIDMQLKLVAGNSAGTVTAYYLSSEGKTHDEIDFEFLGNVSGQPY
Sbjct: 61  VSGSGFRSKREYLFGRIDMQLKLVAGNSAGTVTAYYLSSEGKTHDEIDFEFLGNVSGQPY 120

Query: 121 TLHTNVFTKGKGDREQQFHLWFDPTLKFHTYSVAWTP 158
           TLHTNVFTKGKGDREQQFHLWFDPTLKFHTYSVAWTP
Sbjct: 121 TLHTNVFTKGKGDREQQFHLWFDPTLKFHTYSVAWTP 157

[Close]

BLAST of Spo12227.1 vs. NCBI nr
Match: gi|731325156|ref|XP_010673363.1| (PREDICTED: brassinosteroid-regulated protein BRU1-like [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 275.0 bits (702), Expect = 8.100e-71
Identity = 140/158 (88.61%), Postives = 147/158 (93.04%), Query Frame = 1


		  
Query: 1   MGVSSNGVLVII-VMVVALALSTITSVNGGGNFEQDFEVTFGDGRVKKFWRGQLLTLSLD 60
           MGVSSNG LV+I VMV  LAL +I  VNGG NF+QDFEVTFGDGR+K+F RGQLLTLSLD
Sbjct: 1   MGVSSNGFLVMIMVMVATLALCSIC-VNGG-NFDQDFEVTFGDGRLKRFGRGQLLTLSLD 60

Query: 61  KVSGSGFRSKREYLFGRIDMQLKLVAGNSAGTVTAYYLSSEGKTHDEIDFEFLGNVSGQP 120
           KVSGSGFRSKR+YLFGRIDMQLKLVAGNSAGTVTAYYLSSEG THDEIDFEFLGNVSGQP
Sbjct: 61  KVSGSGFRSKRQYLFGRIDMQLKLVAGNSAGTVTAYYLSSEGATHDEIDFEFLGNVSGQP 120

Query: 121 YTLHTNVFTKGKGDREQQFHLWFDPTLKFHTYSVAWTP 158
           YTLHTNVFT+GKG REQQFHLWFDPTLKFHTYSVAWTP
Sbjct: 121 YTLHTNVFTQGKGGREQQFHLWFDPTLKFHTYSVAWTP 156

[Close]

BLAST of Spo12227.1 vs. NCBI nr
Match: gi|764573164|ref|XP_011462834.1| (PREDICTED: xyloglucan endotransglucosylase/hydrolase 2-like [Fragaria vesca subsp. vesca])

HSP 1 Score: 231.5 bits (589), Expect = 1.000e-57
Identity = 112/157 (71.34%), Postives = 131/157 (83.44%), Query Frame = 1


		  
Query: 1   MGVSSNGVLVIIVMVVALALSTITSVNGGGNFEQDFEVTFGDGRVKKFWRGQLLTLSLDK 60
           MG+S NG    +V+++ LALS+    +  GNF QDF++T+G  R K F  GQLL+LSLDK
Sbjct: 1   MGISGNGAW--LVLLIGLALSSFIVGSYAGNFYQDFDLTWGGNRPKIFKGGQLLSLSLDK 60

Query: 61  VSGSGFRSKREYLFGRIDMQLKLVAGNSAGTVTAYYLSSEGKTHDEIDFEFLGNVSGQPY 120
           VSGSGF+SK+EYLFGRIDMQLKLVAGNSAGTVTAYYLSS+G THDEIDFEFLGNVSG PY
Sbjct: 61  VSGSGFQSKKEYLFGRIDMQLKLVAGNSAGTVTAYYLSSQGPTHDEIDFEFLGNVSGDPY 120

Query: 121 TLHTNVFTKGKGDREQQFHLWFDPTLKFHTYSVAWTP 158
            LHTNVFT+GKG+REQQF+LWFDP+  FHTYS+ W P
Sbjct: 121 VLHTNVFTQGKGNREQQFYLWFDPSKNFHTYSIIWKP 155

[Close]

BLAST of Spo12227.1 vs. NCBI nr
Match: gi|482578119|gb|AFR46580.2| (xyloglucan endotransglucosylase/hydrolase 12 [Rosa x borboniana])

HSP 1 Score: 228.8 bits (582), Expect = 6.700e-57
Identity = 111/157 (70.70%), Postives = 132/157 (84.08%), Query Frame = 1


		  
Query: 1   MGVSSNGVLVIIVMVVALALSTITSVNGGGNFEQDFEVTFGDGRVKKFWRGQLLTLSLDK 60
           MG+S N  +  +V++V LALS++   +  GNF QDF++T+G  R K F  GQLL+LSLDK
Sbjct: 1   MGISGN--VSCLVLLVGLALSSLIVGSYAGNFYQDFDLTWGGHRPKIFKGGQLLSLSLDK 60

Query: 61  VSGSGFRSKREYLFGRIDMQLKLVAGNSAGTVTAYYLSSEGKTHDEIDFEFLGNVSGQPY 120
           VSGSGF+SK+EYLFGRIDMQLKLVAGNSAGTVTAYYLSS+G THDEIDFEFLGNVSG PY
Sbjct: 61  VSGSGFQSKKEYLFGRIDMQLKLVAGNSAGTVTAYYLSSQGPTHDEIDFEFLGNVSGDPY 120

Query: 121 TLHTNVFTKGKGDREQQFHLWFDPTLKFHTYSVAWTP 158
            LHTN+FT+GKG+REQQF+LWFDP+  FHTYS+ W P
Sbjct: 121 VLHTNIFTQGKGNREQQFYLWFDPSKNFHTYSIIWKP 155

[Close]

BLAST of Spo12227.1 vs. NCBI nr
Match: gi|1021489362|ref|XP_016187686.1| (PREDICTED: xyloglucan endotransglucosylase/hydrolase 2-like [Arachis ipaensis])

HSP 1 Score: 228.8 bits (582), Expect = 6.700e-57
Identity = 110/152 (72.37%), Postives = 128/152 (84.21%), Query Frame = 1


		  
Query: 6   NGVLVIIVMVVALALSTITSVNGGGNFEQDFEVTFGDGRVKKFWRGQLLTLSLDKVSGSG 65
           NG  V I+++  + +ST+   +  GNF Q+F++T+G  R K F  GQLL+LSLD+VSGSG
Sbjct: 9   NGFYVGIMVIGLVVVSTMVG-SCEGNFNQEFDLTWGGNRAKIFGGGQLLSLSLDRVSGSG 68

Query: 66  FRSKREYLFGRIDMQLKLVAGNSAGTVTAYYLSSEGKTHDEIDFEFLGNVSGQPYTLHTN 125
           F+SKREYLFGRIDMQLKLVAGNSAGTVTAYYLSS+G THDEIDFEFLGN+SG PYTLHTN
Sbjct: 69  FQSKREYLFGRIDMQLKLVAGNSAGTVTAYYLSSQGPTHDEIDFEFLGNLSGDPYTLHTN 128

Query: 126 VFTKGKGDREQQFHLWFDPTLKFHTYSVAWTP 158
           +FT+GKGDREQQFHLWFDPT  FHTYSV W P
Sbjct: 129 IFTQGKGDREQQFHLWFDPTKNFHTYSVIWKP 159

[Close]

BLAST of Spo12227.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QKY1_SPIOL (Xyloglucan endotransglucosylase/hydrolase OS=Spinacia oleracea GN=SOVF_173740 PE=3 SV=1)

HSP 1 Score: 320.9 bits (821), Expect = 9.000e-85
Identity = 157/157 (100.00%), Postives = 157/157 (100.00%), Query Frame = 1


		  
Query: 1   MGVSSNGVLVIIVMVVALALSTITSVNGGGNFEQDFEVTFGDGRVKKFWRGQLLTLSLDK 60
           MGVSSNGVLVIIVMVVALALSTITSVNGGGNFEQDFEVTFGDGRVKKFWRGQLLTLSLDK
Sbjct: 1   MGVSSNGVLVIIVMVVALALSTITSVNGGGNFEQDFEVTFGDGRVKKFWRGQLLTLSLDK 60

Query: 61  VSGSGFRSKREYLFGRIDMQLKLVAGNSAGTVTAYYLSSEGKTHDEIDFEFLGNVSGQPY 120
           VSGSGFRSKREYLFGRIDMQLKLVAGNSAGTVTAYYLSSEGKTHDEIDFEFLGNVSGQPY
Sbjct: 61  VSGSGFRSKREYLFGRIDMQLKLVAGNSAGTVTAYYLSSEGKTHDEIDFEFLGNVSGQPY 120

Query: 121 TLHTNVFTKGKGDREQQFHLWFDPTLKFHTYSVAWTP 158
           TLHTNVFTKGKGDREQQFHLWFDPTLKFHTYSVAWTP
Sbjct: 121 TLHTNVFTKGKGDREQQFHLWFDPTLKFHTYSVAWTP 157

[Close]

BLAST of Spo12227.1 vs. UniProtKB/TrEMBL
Match: A0A0J8CRX9_BETVU (Xyloglucan endotransglucosylase/hydrolase OS=Beta vulgaris subsp. vulgaris GN=BVRB_3g065250 PE=3 SV=1)

HSP 1 Score: 275.0 bits (702), Expect = 5.600e-71
Identity = 140/158 (88.61%), Postives = 147/158 (93.04%), Query Frame = 1


		  
Query: 1   MGVSSNGVLVII-VMVVALALSTITSVNGGGNFEQDFEVTFGDGRVKKFWRGQLLTLSLD 60
           MGVSSNG LV+I VMV  LAL +I  VNGG NF+QDFEVTFGDGR+K+F RGQLLTLSLD
Sbjct: 1   MGVSSNGFLVMIMVMVATLALCSIC-VNGG-NFDQDFEVTFGDGRLKRFGRGQLLTLSLD 60

Query: 61  KVSGSGFRSKREYLFGRIDMQLKLVAGNSAGTVTAYYLSSEGKTHDEIDFEFLGNVSGQP 120
           KVSGSGFRSKR+YLFGRIDMQLKLVAGNSAGTVTAYYLSSEG THDEIDFEFLGNVSGQP
Sbjct: 61  KVSGSGFRSKRQYLFGRIDMQLKLVAGNSAGTVTAYYLSSEGATHDEIDFEFLGNVSGQP 120

Query: 121 YTLHTNVFTKGKGDREQQFHLWFDPTLKFHTYSVAWTP 158
           YTLHTNVFT+GKG REQQFHLWFDPTLKFHTYSVAWTP
Sbjct: 121 YTLHTNVFTQGKGGREQQFHLWFDPTLKFHTYSVAWTP 156

[Close]

BLAST of Spo12227.1 vs. UniProtKB/TrEMBL
Match: J9S7C7_9ROSA (Xyloglucan endotransglucosylase/hydrolase OS=Rosa x borboniana GN=XTH12 PE=2 SV=2)

HSP 1 Score: 228.8 bits (582), Expect = 4.600e-57
Identity = 111/157 (70.70%), Postives = 132/157 (84.08%), Query Frame = 1


		  
Query: 1   MGVSSNGVLVIIVMVVALALSTITSVNGGGNFEQDFEVTFGDGRVKKFWRGQLLTLSLDK 60
           MG+S N  +  +V++V LALS++   +  GNF QDF++T+G  R K F  GQLL+LSLDK
Sbjct: 1   MGISGN--VSCLVLLVGLALSSLIVGSYAGNFYQDFDLTWGGHRPKIFKGGQLLSLSLDK 60

Query: 61  VSGSGFRSKREYLFGRIDMQLKLVAGNSAGTVTAYYLSSEGKTHDEIDFEFLGNVSGQPY 120
           VSGSGF+SK+EYLFGRIDMQLKLVAGNSAGTVTAYYLSS+G THDEIDFEFLGNVSG PY
Sbjct: 61  VSGSGFQSKKEYLFGRIDMQLKLVAGNSAGTVTAYYLSSQGPTHDEIDFEFLGNVSGDPY 120

Query: 121 TLHTNVFTKGKGDREQQFHLWFDPTLKFHTYSVAWTP 158
            LHTN+FT+GKG+REQQF+LWFDP+  FHTYS+ W P
Sbjct: 121 VLHTNIFTQGKGNREQQFYLWFDPSKNFHTYSIIWKP 155

[Close]

BLAST of Spo12227.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RRH7_SPIOL (Xyloglucan endotransglucosylase/hydrolase OS=Spinacia oleracea GN=SOVF_036570 PE=3 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 6.100e-57
Identity = 106/150 (70.67%), Postives = 121/150 (80.67%), Query Frame = 1


		  
Query: 8   VLVIIVMVVALALSTITSVNGGGNFEQDFEVTFGDGRVKKFWRGQLLTLSLDKVSGSGFR 67
           V  ++ +++ +  S I   +  GNF QDF++T+GDGR K    GQLLTLSLDK SGSGFR
Sbjct: 3   VFALVNVLLLVVTSFIAVASAAGNFNQDFDITWGDGRAKILNNGQLLTLSLDKTSGSGFR 62

Query: 68  SKREYLFGRIDMQLKLVAGNSAGTVTAYYLSSEGKTHDEIDFEFLGNVSGQPYTLHTNVF 127
           SK EYLFG+ID+Q+KLV GNSAGTVT YYLSSEGK HDEIDFEFLGNV+GQPYTLHTNVF
Sbjct: 63  SKNEYLFGKIDLQIKLVPGNSAGTVTTYYLSSEGKNHDEIDFEFLGNVTGQPYTLHTNVF 122

Query: 128 TKGKGDREQQFHLWFDPTLKFHTYSVAWTP 158
            KGKGDREQQF LWFDPT  FHTYS+ W P
Sbjct: 123 AKGKGDREQQFRLWFDPTADFHTYSILWNP 152

[Close]

BLAST of Spo12227.1 vs. UniProtKB/TrEMBL
Match: A0MMD7_LITCN (Xyloglucan endotransglucosylase/hydrolase OS=Litchi chinensis GN=XET3 PE=2 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 6.100e-57
Identity = 105/128 (82.03%), Postives = 116/128 (90.62%), Query Frame = 1


		  
Query: 30  GNFEQDFEVTFGDGRVKKFWRGQLLTLSLDKVSGSGFRSKREYLFGRIDMQLKLVAGNSA 89
           GNF QDF++T+GD R K F RGQLL+LSLDKVSGSGFRSK+EY+FGRIDMQLKLVAGNSA
Sbjct: 8   GNFYQDFDLTWGDHRAKIFNRGQLLSLSLDKVSGSGFRSKKEYMFGRIDMQLKLVAGNSA 67

Query: 90  GTVTAYYLSSEGKTHDEIDFEFLGNVSGQPYTLHTNVFTKGKGDREQQFHLWFDPTLKFH 149
           GTVTAYYLSS+G THDEIDFEFLGN+SG PY LHTNVFT+GKGDREQQF+LWFDPT  FH
Sbjct: 68  GTVTAYYLSSQGPTHDEIDFEFLGNLSGDPYILHTNVFTQGKGDREQQFYLWFDPTRNFH 127

Query: 150 TYSVAWTP 158
           TYS+ W P
Sbjct: 128 TYSIIWKP 135

[Close]

BLAST of Spo12227.1 vs. ExPASy Swiss-Prot
Match: XTH2_SOYBN (Xyloglucan endotransglucosylase/hydrolase 2 OS=Glycine max PE=2 SV=1)

HSP 1 Score: 221.9 bits (564), Expect = 5.100e-57
Identity = 104/146 (71.23%), Postives = 125/146 (85.62%), Query Frame = 1


		  
Query: 12  IVMVVALALSTITSVNGGGNFEQDFEVTFGDGRVKKFWRGQLLTLSLDKVSGSGFRSKRE 71
           ++M+V + +ST+ +   G +F QDF++T+G  R K F  GQLL+LSLDKVSGSGF+SK+E
Sbjct: 13  VLMLVGIVVSTMVATCAG-SFYQDFDLTWGGDRAKIFNGGQLLSLSLDKVSGSGFKSKKE 72

Query: 72  YLFGRIDMQLKLVAGNSAGTVTAYYLSSEGKTHDEIDFEFLGNVSGQPYTLHTNVFTKGK 131
           YLFGRIDMQLKLVAGNSAGTVTAYYLSS+G THDEIDFEFLGN+SG PY LHTN+FT+GK
Sbjct: 73  YLFGRIDMQLKLVAGNSAGTVTAYYLSSQGPTHDEIDFEFLGNLSGDPYILHTNIFTQGK 132

Query: 132 GDREQQFHLWFDPTLKFHTYSVAWTP 158
           G+REQQF+LWFDPT  FHTYS+ W P
Sbjct: 133 GNREQQFYLWFDPTRNFHTYSIIWKP 157

[Close]

BLAST of Spo12227.1 vs. ExPASy Swiss-Prot
Match: XTH23_ARATH (Probable xyloglucan endotransglucosylase/hydrolase protein 23 OS=Arabidopsis thaliana GN=XTH23 PE=2 SV=1)

HSP 1 Score: 212.2 bits (539), Expect = 4.000e-54
Identity = 103/145 (71.03%), Postives = 117/145 (80.69%), Query Frame = 1


		  
Query: 13  VMVVALALSTITSVNGGGNFEQDFEVTFGDGRVKKFWRGQLLTLSLDKVSGSGFRSKREY 72
           ++V  LA   I SV+   NF++D E+T+GDGR +    G LLTLSLDK SGSGF+SK EY
Sbjct: 9   IVVALLASFMICSVSA--NFQRDVEITWGDGRGQITNNGDLLTLSLDKASGSGFQSKNEY 68

Query: 73  LFGRIDMQLKLVAGNSAGTVTAYYLSSEGKTHDEIDFEFLGNVSGQPYTLHTNVFTKGKG 132
           LFG+IDMQ+KLVAGNSAGTVTAYYL S G T DEIDFEFLGN+SG PYTLHTNVFT+GKG
Sbjct: 69  LFGKIDMQIKLVAGNSAGTVTAYYLKSPGSTWDEIDFEFLGNLSGDPYTLHTNVFTQGKG 128

Query: 133 DREQQFHLWFDPTLKFHTYSVAWTP 158
           DREQQF LWFDPT  FHTYS+ W P
Sbjct: 129 DREQQFKLWFDPTSDFHTYSILWNP 151

[Close]

BLAST of Spo12227.1 vs. ExPASy Swiss-Prot
Match: XTH25_ARATH (Probable xyloglucan endotransglucosylase/hydrolase protein 25 OS=Arabidopsis thaliana GN=XTH25 PE=2 SV=2)

HSP 1 Score: 211.8 bits (538), Expect = 5.300e-54
Identity = 96/149 (64.43%), Postives = 122/149 (81.88%), Query Frame = 1


		  
Query: 9   LVIIVMVVALALSTITSVNGGGNFEQDFEVTFGDGRVKKFWRGQLLTLSLDKVSGSGFRS 68
           ++ ++  + ++ +T+ S    G F+ +F++T+GDGR K    G+LLTLSLD+ SGSGF++
Sbjct: 7   ILSLLFTLTVSTTTLFSPVFAGTFDTEFDITWGDGRGKVLNNGELLTLSLDRASGSGFQT 66

Query: 69  KREYLFGRIDMQLKLVAGNSAGTVTAYYLSSEGKTHDEIDFEFLGNVSGQPYTLHTNVFT 128
           K+EYLFG+IDMQLKLV GNSAGTVTAYYL S+G T DEIDFEFLGN++G PYT+HTNV+T
Sbjct: 67  KKEYLFGKIDMQLKLVPGNSAGTVTAYYLKSKGDTWDEIDFEFLGNLTGDPYTMHTNVYT 126

Query: 129 KGKGDREQQFHLWFDPTLKFHTYSVAWTP 158
           +GKGDREQQFHLWFDPT  FHTYSV W P
Sbjct: 127 QGKGDREQQFHLWFDPTADFHTYSVLWNP 155

[Close]

BLAST of Spo12227.1 vs. ExPASy Swiss-Prot
Match: XTH15_ARATH (Xyloglucan endotransglucosylase/hydrolase protein 15 OS=Arabidopsis thaliana GN=XTH15 PE=1 SV=1)

HSP 1 Score: 211.5 bits (537), Expect = 6.900e-54
Identity = 103/157 (65.61%), Postives = 123/157 (78.34%), Query Frame = 1


		  
Query: 1   MGVSSNGVLVIIVMVVALALSTITSVNGGGNFEQDFEVTFGDGRVKKFWRGQLLTLSLDK 60
           MG SS+    +  +V  + L T+       NF  +F++T+GD R K F  G +L+LSLD+
Sbjct: 1   MGPSSS----LTTIVATVLLVTLFGSAYASNFFDEFDLTWGDHRGKIFNGGNMLSLSLDQ 60

Query: 61  VSGSGFRSKREYLFGRIDMQLKLVAGNSAGTVTAYYLSSEGKTHDEIDFEFLGNVSGQPY 120
           VSGSGF+SK+EYLFGRIDMQLKLVAGNSAGTVTAYYLSS+G THDEIDFEFLGN +G+PY
Sbjct: 61  VSGSGFKSKKEYLFGRIDMQLKLVAGNSAGTVTAYYLSSQGATHDEIDFEFLGNETGKPY 120

Query: 121 TLHTNVFTKGKGDREQQFHLWFDPTLKFHTYSVAWTP 158
            LHTNVF +GKGDREQQF+LWFDPT  FHTYS+ W P
Sbjct: 121 VLHTNVFAQGKGDREQQFYLWFDPTKNFHTYSIVWRP 153

[Close]

BLAST of Spo12227.1 vs. ExPASy Swiss-Prot
Match: XTH16_ARATH (Probable xyloglucan endotransglucosylase/hydrolase protein 16 OS=Arabidopsis thaliana GN=XTH16 PE=2 SV=2)

HSP 1 Score: 210.7 bits (535), Expect = 1.200e-53
Identity = 101/157 (64.33%), Postives = 128/157 (81.53%), Query Frame = 1


		  
Query: 1   MGVSSNGVLVIIVMVVALALSTITSVNGGGNFEQDFEVTFGDGRVKKFWRGQLLTLSLDK 60
           MG   N  +++ ++VV +A +  +     G+F ++F++T+G+ R K F  G++L+LSLD+
Sbjct: 1   MGRILNRTVLMTLLVVTMAGTAFS-----GSFNEEFDLTWGEHRGKIFSGGKMLSLSLDR 60

Query: 61  VSGSGFRSKREYLFGRIDMQLKLVAGNSAGTVTAYYLSSEGKTHDEIDFEFLGNVSGQPY 120
           VSGSGF+SK+EYLFGRIDMQLKLVAGNSAGTVTAYYLSSEG THDEIDFEFLGN +G+PY
Sbjct: 61  VSGSGFKSKKEYLFGRIDMQLKLVAGNSAGTVTAYYLSSEGPTHDEIDFEFLGNETGKPY 120

Query: 121 TLHTNVFTKGKGDREQQFHLWFDPTLKFHTYSVAWTP 158
            LHTNVF +GKG+REQQF+LWFDPT  FHTYS+ W P
Sbjct: 121 VLHTNVFAQGKGNREQQFYLWFDPTKNFHTYSLVWRP 152

[Close]

BLAST of Spo12227.1 vs. TAIR (Arabidopsis)
Match: AT4G25810.1 (xyloglucan endotransglycosylase 6)

HSP 1 Score: 212.2 bits (539), Expect = 2.300e-55
Identity = 103/145 (71.03%), Postives = 117/145 (80.69%), Query Frame = 1


		  
Query: 13  VMVVALALSTITSVNGGGNFEQDFEVTFGDGRVKKFWRGQLLTLSLDKVSGSGFRSKREY 72
           ++V  LA   I SV+   NF++D E+T+GDGR +    G LLTLSLDK SGSGF+SK EY
Sbjct: 9   IVVALLASFMICSVSA--NFQRDVEITWGDGRGQITNNGDLLTLSLDKASGSGFQSKNEY 68

Query: 73  LFGRIDMQLKLVAGNSAGTVTAYYLSSEGKTHDEIDFEFLGNVSGQPYTLHTNVFTKGKG 132
           LFG+IDMQ+KLVAGNSAGTVTAYYL S G T DEIDFEFLGN+SG PYTLHTNVFT+GKG
Sbjct: 69  LFGKIDMQIKLVAGNSAGTVTAYYLKSPGSTWDEIDFEFLGNLSGDPYTLHTNVFTQGKG 128

Query: 133 DREQQFHLWFDPTLKFHTYSVAWTP 158
           DREQQF LWFDPT  FHTYS+ W P
Sbjct: 129 DREQQFKLWFDPTSDFHTYSILWNP 151

[Close]

BLAST of Spo12227.1 vs. TAIR (Arabidopsis)
Match: AT5G57550.1 (xyloglucan endotransglucosylase/hydrolase 25)

HSP 1 Score: 211.8 bits (538), Expect = 3.000e-55
Identity = 96/149 (64.43%), Postives = 122/149 (81.88%), Query Frame = 1


		  
Query: 9   LVIIVMVVALALSTITSVNGGGNFEQDFEVTFGDGRVKKFWRGQLLTLSLDKVSGSGFRS 68
           ++ ++  + ++ +T+ S    G F+ +F++T+GDGR K    G+LLTLSLD+ SGSGF++
Sbjct: 7   ILSLLFTLTVSTTTLFSPVFAGTFDTEFDITWGDGRGKVLNNGELLTLSLDRASGSGFQT 66

Query: 69  KREYLFGRIDMQLKLVAGNSAGTVTAYYLSSEGKTHDEIDFEFLGNVSGQPYTLHTNVFT 128
           K+EYLFG+IDMQLKLV GNSAGTVTAYYL S+G T DEIDFEFLGN++G PYT+HTNV+T
Sbjct: 67  KKEYLFGKIDMQLKLVPGNSAGTVTAYYLKSKGDTWDEIDFEFLGNLTGDPYTMHTNVYT 126

Query: 129 KGKGDREQQFHLWFDPTLKFHTYSVAWTP 158
           +GKGDREQQFHLWFDPT  FHTYSV W P
Sbjct: 127 QGKGDREQQFHLWFDPTADFHTYSVLWNP 155

[Close]

BLAST of Spo12227.1 vs. TAIR (Arabidopsis)
Match: AT4G14130.1 (xyloglucan endotransglucosylase/hydrolase 15)

HSP 1 Score: 211.5 bits (537), Expect = 3.900e-55
Identity = 103/157 (65.61%), Postives = 123/157 (78.34%), Query Frame = 1


		  
Query: 1   MGVSSNGVLVIIVMVVALALSTITSVNGGGNFEQDFEVTFGDGRVKKFWRGQLLTLSLDK 60
           MG SS+    +  +V  + L T+       NF  +F++T+GD R K F  G +L+LSLD+
Sbjct: 1   MGPSSS----LTTIVATVLLVTLFGSAYASNFFDEFDLTWGDHRGKIFNGGNMLSLSLDQ 60

Query: 61  VSGSGFRSKREYLFGRIDMQLKLVAGNSAGTVTAYYLSSEGKTHDEIDFEFLGNVSGQPY 120
           VSGSGF+SK+EYLFGRIDMQLKLVAGNSAGTVTAYYLSS+G THDEIDFEFLGN +G+PY
Sbjct: 61  VSGSGFKSKKEYLFGRIDMQLKLVAGNSAGTVTAYYLSSQGATHDEIDFEFLGNETGKPY 120

Query: 121 TLHTNVFTKGKGDREQQFHLWFDPTLKFHTYSVAWTP 158
            LHTNVF +GKGDREQQF+LWFDPT  FHTYS+ W P
Sbjct: 121 VLHTNVFAQGKGDREQQFYLWFDPTKNFHTYSIVWRP 153

[Close]

BLAST of Spo12227.1 vs. TAIR (Arabidopsis)
Match: AT3G23730.1 (xyloglucan endotransglucosylase/hydrolase 16)

HSP 1 Score: 210.7 bits (535), Expect = 6.600e-55
Identity = 101/157 (64.33%), Postives = 128/157 (81.53%), Query Frame = 1


		  
Query: 1   MGVSSNGVLVIIVMVVALALSTITSVNGGGNFEQDFEVTFGDGRVKKFWRGQLLTLSLDK 60
           MG   N  +++ ++VV +A +  +     G+F ++F++T+G+ R K F  G++L+LSLD+
Sbjct: 1   MGRILNRTVLMTLLVVTMAGTAFS-----GSFNEEFDLTWGEHRGKIFSGGKMLSLSLDR 60

Query: 61  VSGSGFRSKREYLFGRIDMQLKLVAGNSAGTVTAYYLSSEGKTHDEIDFEFLGNVSGQPY 120
           VSGSGF+SK+EYLFGRIDMQLKLVAGNSAGTVTAYYLSSEG THDEIDFEFLGN +G+PY
Sbjct: 61  VSGSGFKSKKEYLFGRIDMQLKLVAGNSAGTVTAYYLSSEGPTHDEIDFEFLGNETGKPY 120

Query: 121 TLHTNVFTKGKGDREQQFHLWFDPTLKFHTYSVAWTP 158
            LHTNVF +GKG+REQQF+LWFDPT  FHTYS+ W P
Sbjct: 121 VLHTNVFAQGKGNREQQFYLWFDPTKNFHTYSLVWRP 152

[Close]

BLAST of Spo12227.1 vs. TAIR (Arabidopsis)
Match: AT4G25820.1 (xyloglucan endotransglucosylase/hydrolase 14)

HSP 1 Score: 208.8 bits (530), Expect = 2.500e-54
Identity = 97/147 (65.99%), Postives = 116/147 (78.91%), Query Frame = 1


		  
Query: 12  IVMVVALALSTITSVNGGGNFEQDFEVTFGDGRVKKFWRGQLLTLSLDKVSGSGFRSKRE 71
           +++ + LA+         GNF + F++T+G+GR   F  GQLLT +LDKVSGSGF+SK+E
Sbjct: 10  LLLSLLLAIGFFVVAASAGNFYESFDITWGNGRANIFENGQLLTCTLDKVSGSGFQSKKE 69

Query: 72  YLFGRIDMQLKLVAGNSAGTVTAYYLSSEGKTHDEIDFEFLGNVSGQPYTLHTNVFTKGK 131
           YLFG+IDM+LKLVAGNSAGTVTAYYLSS+G   DEIDFEFLGN +G PYT+HTNVFT GK
Sbjct: 70  YLFGKIDMKLKLVAGNSAGTVTAYYLSSKGTAWDEIDFEFLGNRTGHPYTIHTNVFTGGK 129

Query: 132 GDREQQFHLWFDPTLKFHTYSVAWTPV 159
           GDRE QF LWFDPT  FHTY+V W PV
Sbjct: 130 GDREMQFRLWFDPTADFHTYTVHWNPV 156

The following BLAST results are available for this feature:

BLAST of Spo12227.1 vs. NCBI nr
BLAST of Spo12227.1 vs. UniProtKB/TrEMBL
BLAST of Spo12227.1 vs. ExPASy Swiss-Prot
BLAST of Spo12227.1 vs. TAIR (Arabidopsis)

BLAST of Spo12227.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902166498\|gb\|KNA07231.1\|	1.3e-84	100.	hypothetical protein SOVF_1737...	[more]
gi\|731325156\|ref\|XP_010673363.1\|	8.1e-71	88.6	PREDICTED: brassinosteroid-reg...	[more]
gi\|764573164\|ref\|XP_011462834.1\|	1.0e-57	71.3	PREDICTED: xyloglucan endotran...	[more]
gi\|482578119\|gb\|AFR46580.2\|	6.7e-57	70.7	xyloglucan endotransglucosylas...	[more]
gi\|1021489362\|ref\|XP_016187686.1\|	6.7e-57	72.3	PREDICTED: xyloglucan endotran...	[more]

BLAST of Spo12227.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9QKY1_SPIOL	9.0e-85	100.	Xyloglucan endotransglucosylas...	[more]
A0A0J8CRX9_BETVU	5.6e-71	88.6	Xyloglucan endotransglucosylas...	[more]
J9S7C7_9ROSA	4.6e-57	70.7	Xyloglucan endotransglucosylas...	[more]
A0A0K9RRH7_SPIOL	6.1e-57	70.6	Xyloglucan endotransglucosylas...	[more]
A0MMD7_LITCN	6.1e-57	82.0	Xyloglucan endotransglucosylas...	[more]

BLAST of Spo12227.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
XTH2_SOYBN	5.1e-57	71.2	Xyloglucan endotransglucosylas...	[more]
XTH23_ARATH	4.0e-54	71.0	Probable xyloglucan endotransg...	[more]
XTH25_ARATH	5.3e-54	64.4	Probable xyloglucan endotransg...	[more]
XTH15_ARATH	6.9e-54	65.6	Xyloglucan endotransglucosylas...	[more]
XTH16_ARATH	1.2e-53	64.3	Probable xyloglucan endotransg...	[more]

BLAST of Spo12227.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 5


Match Name	E-value	Identity	Description
AT4G25810.1	2.3e-55	71.0	xyloglucan endotransglycosylas...	[more]
AT5G57550.1	3.0e-55	64.4	xyloglucan endotransglucosylas...	[more]
AT4G14130.1	3.9e-55	65.6	xyloglucan endotransglucosylas...	[more]
AT3G23730.1	6.6e-55	64.3	xyloglucan endotransglucosylas...	[more]
AT4G25820.1	2.5e-54	65.9	xyloglucan endotransglucosylas...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000757	Glycoside hydrolase family 16	PFAM	PF00722	Glyco_hydro_16	coord: 33..157 score: 9.8
IPR000757	Glycoside hydrolase family 16	PROFILE	PS51762	GH16_2	coord: 26..158 score: 1
IPR008263	Glycoside hydrolase, family 16, active site	PROSITE	PS01034	GH16_1	coord: 106..116 scor
IPR008264	Beta-glucanase	PRINTS	PR00737	GLHYDRLASE16	coord: 122..135 score: 5.3E-5coord: 64..82 score: 5.3E-5coord: 140..157 score: 5.
IPR013320	Concanavalin A-like lectin/glucanase domain	GENE3D	2.60.120.200		coord: 25..157 score: 4.6
IPR013320	Concanavalin A-like lectin/glucanase domain	unknown	SSF49899	Concanavalin A-like lectins/glucanases	coord: 31..157 score: 4.3
None	No IPR available	PANTHER	PTHR31062	FAMILY NOT NAMED	coord: 1..157 score: 6.2
None	No IPR available	PANTHER	PTHR31062:SF20	XYLOGLUCAN ENDOTRANSGLUCOSYLASE/HYDROLASE PROTEIN 14-RELATED	coord: 1..157 score: 6.2

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
biological_process	GO:0006073	cellular glucan metabolic process
biological_process	GO:0071555	cell wall organization
cellular_component	GO:0048046	apoplast
cellular_component	GO:0005618	cell wall
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds
molecular_function	GO:0016762	xyloglucan:xyloglucosyl transferase activity