Spo12965.1 (mRNA) | SpinachBase

Overview


Name	Spo12965.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	Membrane steroid-binding protein
Location	chr3 : 2157612 .. 2161263 (-)
Sequence length	1855

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AATCGAGCCCTACACAACACATAAACACATATCAAAATCGACACAAAATAAAACACGAACGCACCTCGTAGCCCTAATTGAACTGAAAAAATGTTCCTCTTTAACTCATTGATTTGACTTTGACGGTCTCACCTGCCTACTATTCAAGTACCCTCGTAATTCAAACTGCGACACATACCCAACTCAAACCTGACACAACACTCACAAACACGGGTCAAATTCTCAAACTACGACACAAACCCAACTCAAACCTAACACAACACTCACAAACACGGGTCAAATTCTAAAACTACGACACATACCCAATTCAAACCTGACACAACACTCACAAACACGAGTCAAATTCTCAAACTACGACACATACCCAACTCAAACCTGACACAACACTCATAAACACGGGTCAAATTCTCAAATTACGACACATACCCAACTCAAACCTGACACGACACTCACAAACATGTGTCAAACTCGACACAAAATCAAACACGAACACACTCTTGAACCTCGTGGGATTGACTCTCATCTACCTATTCAACTGCCCAAGTTCCAACCCACCAATAAACCATCTATCTGTTCTCCCATTCTCCCACCTCCATCCGGCCATCCATCTTCATCACTCTCCTCACCAAATCCCAACACTTTTTCTCTCTCCTCAATCCTCATAAGTCAGAGAGTCACAGACCCCAAATTCAACCCGAACTCTCACAAATTCACAATTAACACACACCTTCTTCAACCTCAACAACCCCTCCATCCTCAAAACCTCACAAAAAATGGCTCTGGAATTATGGGGAACACTCAAAGAAGCAATCACAACATACACAGGTCTTTCTCCAACAACTTTCTTCACTGTAGTTGTTTTAAGTTTAGCCTTTTACCATATGGTTTTTGGGTTGTTTGGATCAACCCCTTCTCCGCATCAACGCCATAGGTCTATGGAGGAAGAGATGGAGCCCTTGCCACCACCGGTTCAGCTTGGGGAGATTGAGGAAGATGAGTTGAAACTCTATGATGGCACTGATCCCAAGAAACCCTTGCTTATGGCTATTAAGGGTCAGATCTACGATGTTTCCCAGAGCAGGTCTAATTTCAATTTTAATTTTAATTTCTTTCTAATGTTTTGTTCTTGGTATTTTAAGTTCTGTGTTGCTGAAATTAGCTGGGGTTTTTGTGATCTGAACTGGGTTAATTCAATTTTTTTAGGGTTTGACTGATTTAGGGTTTTAGTAGCTTTGCATGAAGGTAATATGTTTGATCTTGTGGTTAATTGAAGTTTAAAGTTCTGAACTTTGATAATTTGTGGGTTTCAATTGGGTCAATTAGCTTTTGGGGGACTTTTATTTGTGTGTATCTTTAACTGAGTTTTCTTTGGCTGCCAATTGTTGGGGTTTAGGTATGGCTTAATCTTGTTGTTTTTCATAGGTTTAATTAGTTTCTGGAGTTAAATTTGTGTTTTCATGGTGTTGAAGTTGAAGATACAGTTTTCTGGGATTTGGCAGCTGGATAAGTGGAGAGTGTTATGTGGATTTTTATTTGTTAATCAGTGTGCTGTGGAAGTGTTGATCATAATCCTTGTTTCTGATGTGTTAACTATGGGTGTTTGAAGTCTGGAAATGAATCATATAATGCACAAGTACAAGCTTATCATATAATGCACCACTGTTTTGTTTTCCATTTTCTCTTCCCTAGTTTGTGTTAGACTTTATTGGTAGAAATGTGTTGTTTAGCGGTGTATCTAGCGCCTTTTAAAAGATTAGGGAATCTGAAAAGCACTGGAAAAGGATTTTGGTGGAAGAGTTAAAGAAAAGACAGTAACTTTGTATACTCATATGACTTTCTTTCTCAAAAGATCAGATTATTGAGTAGAATTTGACATTCATTATATGGATGAGGGTAGGTAGTGGATGAAAAAGCCTCCCTTCTTGTTTTGAGTCTTTTGATTGACAAGGGTAAGTTGCACAACTTGCACAGAGTTATAACCCTCCATCCTCACGCACTTCACCAGTAGCGGAATTCTGATACCGTTGTTAGTTGAACCAGTAACCTTTAAATCATATTCAGCCTAAAGTCTCATCCTTTTTTCACTTTTTAATTGCTTTGTTTGTCACTGGAATTGATTCTGCAACTGTTACCGATAACTGCTTTGTTCATCACTGGTTTGTTCTGCTCCTTGACAGTCATTTGGGTTCTAGACTGATAAAGTAATGCTTCCTTTTCAACTTAAACATAGATTTGTGCCACTTCCCAAAAGCAGAAAGGGATCTTATATATTCCTTTTACGGTTTTACCACTTTATTTGAGAATAACTAACTTTTGGATATTGCTTTTATAAAATTGAATCACCCAACTTTTTTTTGCTTCTTTTCAACCTCCCATTTACATAACCATGCGTCTGCAAATCTGTAGTACACGCTGTTCTGATGATACCCTTTTTCTGATGCTATACACCTTGTGAATCATGAAAAATAAGTGAATTCAAACTGCTGGTTGCTCTCCTTGCTTCTTTGTTTTGTTTGTCTAGGTTTTTTTTTGTTTTTTTCCTGGTTTAGTTTAATCTTGCTGGCTGCTGTTTCGGTTTTTCTCGTGGAGTTTCGTTTTGTACGCACTCTTTGCTATTCAAGTGCCTTATCTTCATTTAAGCATATAACTGGTATTCATTGTGCAAATTCTATTTCTTTTCTTCACTGGAGTGAAGTAAATGCCTAAGTATATGCACCTATCTATAGGATCTTTTAGAATATTGTGGATGACATTGCAACTGTCAAGATCTTTGAGCATTCCCGAAACATTTCTGAGATTCACCCTTTTCCATTCCATATAAATACCATAACTGGTGCTATGCAACGAACTATGTGTGCTAACATCCGTGTTGTTCCAGGATGTTCTATGGTCCAGGTGGGCCCTACGCTTTATTCGCCGGCAAAGACGCCAGCCGAGCTCTAGCCAAGATGTCGTTCGAAGATAGCGATTTGACAGGGGATATTTCCGGCCTTGGTCCTTTCGAGCTCGATGCACTACAAGACTGGGAATACAAGTTTATGAGTAAATATGTTAAGGTCGGAACTATTAAGTCAAAGGATGCTCCGGTAACTGATGTATCCTCTCCTACCGAAGCAGCCGAAGCTACTGACGGCGTGTCAGAGAAGCCTGAAGCAGAGACTGTAGAGACACCGGCTACCGATGCTGAGAATAAAGACGAGTAAAACAAACAAAAAACTATGCCGTTGGCTGTATACCGAGTTTTGGAAGAAAAAAAAAAGTTTTTAAGAAGCATGGAAAAAGATATAGAAGGGGGGGCTAAGTAGGTTTGGGGTTAAAGAAGTGTGGAATGATGTGATAAATTGGATCCTTGTTACAAGAACTTGTTTACTTGAATTGCTATTGTAAATAAGCATGCTTGTCTGTGGACTCCATTGAATAAGTTTGATGGTAATACAGCTGAAATTGTTGCAATCTCTCTTTTATTTGGCTTATGTAAGATGGTTGTGAGTTGGGCTGGGCTGGGCTAAGCTTTGTGTTGAGCCCACGTGTTTTCGGTGAACAGGGCCGACTCACGCCAAATCCACTTATTTCATATGGGTTGGCTGTATCGAAATTTTCAAAAAAAATGGCCTAGGCACGGCCCAAACCACGTACCCTCGTGTTGGGTCGG

mRNA sequence

AATCGAGCCCTACACAACACATAAACACATATCAAAATCGACACAAAATAAAACACGAACGCACCTCGTAGCCCTAATTGAACTGAAAAAATGTTCCTCTTTAACTCATTGATTTGACTTTGACGGTCTCACCTGCCTACTATTCAAGTACCCTCGTAATTCAAACTGCGACACATACCCAACTCAAACCTGACACAACACTCACAAACACGGGTCAAATTCTCAAACTACGACACAAACCCAACTCAAACCTAACACAACACTCACAAACACGGGTCAAATTCTAAAACTACGACACATACCCAATTCAAACCTGACACAACACTCACAAACACGAGTCAAATTCTCAAACTACGACACATACCCAACTCAAACCTGACACAACACTCATAAACACGGGTCAAATTCTCAAATTACGACACATACCCAACTCAAACCTGACACGACACTCACAAACATGTGTCAAACTCGACACAAAATCAAACACGAACACACTCTTGAACCTCGTGGGATTGACTCTCATCTACCTATTCAACTGCCCAAGTTCCAACCCACCAATAAACCATCTATCTGTTCTCCCATTCTCCCACCTCCATCCGGCCATCCATCTTCATCACTCTCCTCACCAAATCCCAACACTTTTTCTCTCTCCTCAATCCTCATAAGTCAGAGAGTCACAGACCCCAAATTCAACCCGAACTCTCACAAATTCACAATTAACACACACCTTCTTCAACCTCAACAACCCCTCCATCCTCAAAACCTCACAAAAAATGGCTCTGGAATTATGGGGAACACTCAAAGAAGCAATCACAACATACACAGGTCTTTCTCCAACAACTTTCTTCACTGTAGTTGTTTTAAGTTTAGCCTTTTACCATATGGTTTTTGGGTTGTTTGGATCAACCCCTTCTCCGCATCAACGCCATAGGTCTATGGAGGAAGAGATGGAGCCCTTGCCACCACCGGTTCAGCTTGGGGAGATTGAGGAAGATGAGTTGAAACTCTATGATGGCACTGATCCCAAGAAACCCTTGCTTATGGCTATTAAGGGTCAGATCTACGATGTTTCCCAGAGCAGGATGTTCTATGGTCCAGGTGGGCCCTACGCTTTATTCGCCGGCAAAGACGCCAGCCGAGCTCTAGCCAAGATGTCGTTCGAAGATAGCGATTTGACAGGGGATATTTCCGGCCTTGGTCCTTTCGAGCTCGATGCACTACAAGACTGGGAATACAAGTTTATGAGTAAATATGTTAAGGTCGGAACTATTAAGTCAAAGGATGCTCCGGTAACTGATGTATCCTCTCCTACCGAAGCAGCCGAAGCTACTGACGGCGTGTCAGAGAAGCCTGAAGCAGAGACTGTAGAGACACCGGCTACCGATGCTGAGAATAAAGACGAGTAAAACAAACAAAAAACTATGCCGTTGGCTGTATACCGAGTTTTGGAAGAAAAAAAAAAGTTTTTAAGAAGCATGGAAAAAGATATAGAAGGGGGGGCTAAGTAGGTTTGGGGTTAAAGAAGTGTGGAATGATGTGATAAATTGGATCCTTGTTACAAGAACTTGTTTACTTGAATTGCTATTGTAAATAAGCATGCTTGTCTGTGGACTCCATTGAATAAGTTTGATGGTAATACAGCTGAAATTGTTGCAATCTCTCTTTTATTTGGCTTATGTAAGATGGTTGTGAGTTGGGCTGGGCTGGGCTAAGCTTTGTGTTGAGCCCACGTGTTTTCGGTGAACAGGGCCGACTCACGCCAAATCCACTTATTTCATATGGGTTGGCTGTATCGAAATTTTCAAAAAAAATGGCCTAGGCACGGCCCAAACCACGTACCCTCGTGTTGGGTCGG

Coding sequence (CDS)

ATGGCTCTGGAATTATGGGGAACACTCAAAGAAGCAATCACAACATACACAGGTCTTTCTCCAACAACTTTCTTCACTGTAGTTGTTTTAAGTTTAGCCTTTTACCATATGGTTTTTGGGTTGTTTGGATCAACCCCTTCTCCGCATCAACGCCATAGGTCTATGGAGGAAGAGATGGAGCCCTTGCCACCACCGGTTCAGCTTGGGGAGATTGAGGAAGATGAGTTGAAACTCTATGATGGCACTGATCCCAAGAAACCCTTGCTTATGGCTATTAAGGGTCAGATCTACGATGTTTCCCAGAGCAGGATGTTCTATGGTCCAGGTGGGCCCTACGCTTTATTCGCCGGCAAAGACGCCAGCCGAGCTCTAGCCAAGATGTCGTTCGAAGATAGCGATTTGACAGGGGATATTTCCGGCCTTGGTCCTTTCGAGCTCGATGCACTACAAGACTGGGAATACAAGTTTATGAGTAAATATGTTAAGGTCGGAACTATTAAGTCAAAGGATGCTCCGGTAACTGATGTATCCTCTCCTACCGAAGCAGCCGAAGCTACTGACGGCGTGTCAGAGAAGCCTGAAGCAGAGACTGTAGAGACACCGGCTACCGATGCTGAGAATAAAGACGAGTAA

Protein sequence

MALELWGTLKEAITTYTGLSPTTFFTVVVLSLAFYHMVFGLFGSTPSPHQRHRSMEEEMEPLPPPVQLGEIEEDELKLYDGTDPKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDASRALAKMSFEDSDLTGDISGLGPFELDALQDWEYKFMSKYVKVGTIKSKDAPVTDVSSPTEAAEATDGVSEKPEAETVETPATDAENKDE

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo12965	Spo12965	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo12965.1	Spo12965.1-protein	polypeptide

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo12965.1.utr5p.1	Spo12965.1.utr5p.1	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo12965.1.CDS.2	Spo12965.1.CDS.2	CDS
Spo12965.1.CDS.1	Spo12965.1.CDS.1	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo12965.1.utr3p.1	Spo12965.1.utr3p.1	three_prime_UTR

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo12965.1.exon.2	Spo12965.1.exon.2	exon
Spo12965.1.exon.1	Spo12965.1.exon.1	exon

Homology

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BLAST of Spo12965.1 vs. NCBI nr
Match: gi|902235440|gb|KNA23910.1| (hypothetical protein SOVF_019540 [Spinacia oleracea])

HSP 1 Score: 411.0 bits (1055), Expect = 1.300e-111
Identity = 209/210 (99.52%), Postives = 209/210 (99.52%), Query Frame = 1


		  
Query: 1   MALELWGTLKEAITTYTGLSPTTFFTVVVLSLAFYHMVFGLFGSTPSPHQRHRSMEEEME 60
           MALE WGTLKEAITTYTGLSPTTFFTVVVLSLAFYHMVFGLFGSTPSPHQRHRSMEEEME
Sbjct: 1   MALEFWGTLKEAITTYTGLSPTTFFTVVVLSLAFYHMVFGLFGSTPSPHQRHRSMEEEME 60

Query: 61  PLPPPVQLGEIEEDELKLYDGTDPKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDA 120
           PLPPPVQLGEIEEDELKLYDGTDPKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDA
Sbjct: 61  PLPPPVQLGEIEEDELKLYDGTDPKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDA 120

Query: 121 SRALAKMSFEDSDLTGDISGLGPFELDALQDWEYKFMSKYVKVGTIKSKDAPVTDVSSPT 180
           SRALAKMSFEDSDLTGDISGLGPFELDALQDWEYKFMSKYVKVGTIKSKDAPVTDVSSPT
Sbjct: 121 SRALAKMSFEDSDLTGDISGLGPFELDALQDWEYKFMSKYVKVGTIKSKDAPVTDVSSPT 180

Query: 181 EAAEATDGVSEKPEAETVETPATDAENKDE 211
           EAAEATDGVSEKPEAETVETPATDAENKDE
Sbjct: 181 EAAEATDGVSEKPEAETVETPATDAENKDE 210

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BLAST of Spo12965.1 vs. NCBI nr
Match: gi|731363620|ref|XP_010693524.1| (PREDICTED: membrane steroid-binding protein 2 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 320.5 bits (820), Expect = 2.200e-84
Identity = 170/227 (74.89%), Postives = 182/227 (80.18%), Query Frame = 1


		  
Query: 1   MALELWGTLKEAITTYTGLSPTTFFTVVVLSLAFYHMVFGLFGSTPSPHQRHRSMEEEME 60
           MALELW TLKEAIT YTGLSP TFFTV+ LSLAFYHMV GLFGS+PS + +H    EE+E
Sbjct: 1   MALELWETLKEAITAYTGLSPATFFTVIALSLAFYHMVSGLFGSSPSQNHQHPRSMEEIE 60

Query: 61  PLPPPVQLGEIEEDELKLYDGTDPKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDA 120
           PLPPPVQLGEI EDELK YDG+D KKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDA
Sbjct: 61  PLPPPVQLGEITEDELKNYDGSDSKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDA 120

Query: 121 SRALAKMSFEDSDLTGDISGLGPFELDALQDWEYKFMSKYVKVGTIKSKDAPVTDVSSPT 180
           SRALAKMSFED+DLTGDISGLGPFEL+ALQDWEYKFMSKYVKVGT+K+KD P T  SSP 
Sbjct: 121 SRALAKMSFEDNDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTLKTKDVPATGESSPN 180

Query: 181 EAAEATDGVS-----------------EKPEAETVETPATDAENKDE 211
           E  EATD  +                 EKP AET ET  TDAE K+E
Sbjct: 181 ETVEATDREAPKQEEHPPSEKPEDSPLEKPIAETAETSLTDAEKKEE 227

[Close]

BLAST of Spo12965.1 vs. NCBI nr
Match: gi|702488501|ref|XP_010035120.1| (PREDICTED: membrane steroid-binding protein 2 [Eucalyptus grandis])

HSP 1 Score: 289.3 bits (739), Expect = 5.500e-75
Identity = 153/212 (72.17%), Postives = 168/212 (79.25%), Query Frame = 1


		  
Query: 1   MALELWGTLKEAITTYTGLSPTTFFTVVVLSLAFYHMVFGLFGSTPSPHQRHRSMEEEME 60
           MAL+LW TLKE+I  YTGLSP TFFTV+ L LA Y++   LFGS    HQR R  +E+M+
Sbjct: 1   MALQLWETLKESIVAYTGLSPATFFTVLALGLAVYYVASSLFGSPADHHQRPRDYQEQMQ 60

Query: 61  PLPPPVQLGEIEEDELKLYDGTDPKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDA 120
           PLPPPVQLGEI  +ELK YDG DPKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDA
Sbjct: 61  PLPPPVQLGEISGEELKQYDGADPKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDA 120

Query: 121 SRALAKMSFEDSDLTGDISGLGPFELDALQDWEYKFMSKYVKVGTIKSKDAPVTDVSSPT 180
           SRALAKMSFED DLTGDISGLGPFE+DALQDWEYKFMSKYVKVGTIK K  PVT+  S  
Sbjct: 121 SRALAKMSFEDKDLTGDISGLGPFEIDALQDWEYKFMSKYVKVGTIK-KTVPVTEGESTA 180

Query: 181 EAAE---------ATDGVSEKPEAETVETPAT 204
           E AE         + DG SE  EA+T ETP++
Sbjct: 181 EPAEVADKDVPKTSEDGPSESTEAKTEETPSS 211

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BLAST of Spo12965.1 vs. NCBI nr
Match: gi|743857017|ref|XP_011030067.1| (PREDICTED: membrane steroid-binding protein 2-like [Populus euphratica])

HSP 1 Score: 288.1 bits (736), Expect = 1.200e-74
Identity = 157/217 (72.35%), Postives = 175/217 (80.65%), Query Frame = 1


		  
Query: 1   MALELWGTLKEAITTYTGLSPTTFFTVVVLSLAFYHMVFGLFGSTPSPHQRHRSMEEEME 60
           MALELW T+KEAIT YTGLSP TFFTV  L LA Y++V GLFG +   H   R  EE+M+
Sbjct: 1   MALELWETVKEAITAYTGLSPATFFTVAALGLAVYYVVSGLFGGSDHHHHVPRQSEEQMQ 60

Query: 61  PLPPPVQLGEIEEDELKLYDGTDPKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDA 120
           PLPPPVQLGE+ E+ELK YDGTDPKKPLLMAIKGQIYDV+QSRMFYGPGGPYALFAGKDA
Sbjct: 61  PLPPPVQLGEVTEEELKQYDGTDPKKPLLMAIKGQIYDVTQSRMFYGPGGPYALFAGKDA 120

Query: 121 SRALAKMSFEDSDLTGDISGLGPFELDALQDWEYKFMSKYVKVGTIKSKDAPVTDVSSPT 180
           SRALAKMSFED DLTGD+SGLGPFEL+ALQDWE+KFMSKY+KVGTIK K  PVTD +S +
Sbjct: 121 SRALAKMSFEDKDLTGDVSGLGPFELEALQDWEFKFMSKYIKVGTIK-KTVPVTDGTSTS 180

Query: 181 E-AAEATDGVSEKPE------AETVETPATDAENKDE 211
           E AAEAT+    KP       A  VETPA   E+K+E
Sbjct: 181 EPAAEATESDVAKPAEDVPSVAAPVETPA-GGESKEE 215

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BLAST of Spo12965.1 vs. NCBI nr
Match: gi|255539487|ref|XP_002510808.1| (PREDICTED: membrane steroid-binding protein 1 [Ricinus communis])

HSP 1 Score: 287.3 bits (734), Expect = 2.100e-74
Identity = 154/219 (70.32%), Postives = 173/219 (79.00%), Query Frame = 1


		  
Query: 1   MALELWGTLKEAITTYTGLSPTTFFTVVVLSLAFYHMVFGLFGSTPSPHQRHRSMEEEME 60
           MAL+LW T KEAIT YTGLSP TFFTV+ L LA Y+++ G+FGS+ + HQR RS EE+M+
Sbjct: 3   MALQLWETFKEAITVYTGLSPATFFTVLALGLAVYYVISGMFGSSDTHHQRPRSFEEQMQ 62

Query: 61  PLPPPVQLGEIEEDELKLYDGTDPKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDA 120
           PLPPPVQLGE+ E+ELK YDG+DPKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDA
Sbjct: 63  PLPPPVQLGEVTEEELKQYDGSDPKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDA 122

Query: 121 SRALAKMSFEDSDLTGDISGLGPFELDALQDWEYKFMSKYVKVGTIKSKDAPVTD-VSSP 180
           SRALAKMSFED DLTGDISGLGPFEL+ALQDWEYKFMSKYVKVGTIK K  PVTD  +S 
Sbjct: 123 SRALAKMSFEDKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTIK-KPVPVTDEATSA 182

Query: 181 TEAAEATDGVSEKPE--------AETVETPATDAENKDE 211
           +E AE  +    KP         A+  E P+ D     E
Sbjct: 183 SEPAENKEVDDTKPAEDGPTADVAKPTEGPSADVAKSTE 220

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BLAST of Spo12965.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RWJ8_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_019540 PE=3 SV=1)

HSP 1 Score: 411.0 bits (1055), Expect = 8.800e-112
Identity = 209/210 (99.52%), Postives = 209/210 (99.52%), Query Frame = 1


		  
Query: 1   MALELWGTLKEAITTYTGLSPTTFFTVVVLSLAFYHMVFGLFGSTPSPHQRHRSMEEEME 60
           MALE WGTLKEAITTYTGLSPTTFFTVVVLSLAFYHMVFGLFGSTPSPHQRHRSMEEEME
Sbjct: 1   MALEFWGTLKEAITTYTGLSPTTFFTVVVLSLAFYHMVFGLFGSTPSPHQRHRSMEEEME 60

Query: 61  PLPPPVQLGEIEEDELKLYDGTDPKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDA 120
           PLPPPVQLGEIEEDELKLYDGTDPKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDA
Sbjct: 61  PLPPPVQLGEIEEDELKLYDGTDPKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDA 120

Query: 121 SRALAKMSFEDSDLTGDISGLGPFELDALQDWEYKFMSKYVKVGTIKSKDAPVTDVSSPT 180
           SRALAKMSFEDSDLTGDISGLGPFELDALQDWEYKFMSKYVKVGTIKSKDAPVTDVSSPT
Sbjct: 121 SRALAKMSFEDSDLTGDISGLGPFELDALQDWEYKFMSKYVKVGTIKSKDAPVTDVSSPT 180

Query: 181 EAAEATDGVSEKPEAETVETPATDAENKDE 211
           EAAEATDGVSEKPEAETVETPATDAENKDE
Sbjct: 181 EAAEATDGVSEKPEAETVETPATDAENKDE 210

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BLAST of Spo12965.1 vs. UniProtKB/TrEMBL
Match: A0A0J8BDV2_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_3g066760 PE=3 SV=1)

HSP 1 Score: 320.5 bits (820), Expect = 1.600e-84
Identity = 170/227 (74.89%), Postives = 182/227 (80.18%), Query Frame = 1


		  
Query: 1   MALELWGTLKEAITTYTGLSPTTFFTVVVLSLAFYHMVFGLFGSTPSPHQRHRSMEEEME 60
           MALELW TLKEAIT YTGLSP TFFTV+ LSLAFYHMV GLFGS+PS + +H    EE+E
Sbjct: 1   MALELWETLKEAITAYTGLSPATFFTVIALSLAFYHMVSGLFGSSPSQNHQHPRSMEEIE 60

Query: 61  PLPPPVQLGEIEEDELKLYDGTDPKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDA 120
           PLPPPVQLGEI EDELK YDG+D KKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDA
Sbjct: 61  PLPPPVQLGEITEDELKNYDGSDSKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDA 120

Query: 121 SRALAKMSFEDSDLTGDISGLGPFELDALQDWEYKFMSKYVKVGTIKSKDAPVTDVSSPT 180
           SRALAKMSFED+DLTGDISGLGPFEL+ALQDWEYKFMSKYVKVGT+K+KD P T  SSP 
Sbjct: 121 SRALAKMSFEDNDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTLKTKDVPATGESSPN 180

Query: 181 EAAEATDGVS-----------------EKPEAETVETPATDAENKDE 211
           E  EATD  +                 EKP AET ET  TDAE K+E
Sbjct: 181 ETVEATDREAPKQEEHPPSEKPEDSPLEKPIAETAETSLTDAEKKEE 227

[Close]

BLAST of Spo12965.1 vs. UniProtKB/TrEMBL
Match: G5DXK7_SILLA (Membrane steroid-binding protein (Fragment) OS=Silene latifolia PE=2 SV=1)

HSP 1 Score: 300.4 bits (768), Expect = 1.700e-78
Identity = 163/210 (77.62%), Postives = 172/210 (81.90%), Query Frame = 1


		  
Query: 1   MALELWGTLKEAITTYTGLSPTTFFTVVVLSLAFYHMVFGLFGSTPSP-HQRHRSMEEEM 60
           MALELW TLKEAIT YTGLSP TFFTVV LSLAFYH VFGLFGS+ S  HQ  RS EE+M
Sbjct: 1   MALELWETLKEAITVYTGLSPATFFTVVALSLAFYHAVFGLFGSSSSANHQHPRSFEEQM 60

Query: 61  EPLPPPVQLGEIEEDELKLYDGTDPKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKD 120
           EPLPPPVQLGEI EDELK YDG+D KKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKD
Sbjct: 61  EPLPPPVQLGEITEDELKQYDGSDSKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKD 120

Query: 121 ASRALAKMSFEDSDLTGDISGLGPFELDALQDWEYKFMSKYVKVGTIKSKDAPVTDVSS- 180
           ASRALAKMSFED DLTGDISGLGPFEL+ALQDWEYKFMSKYVKVGTIK   A   + SS 
Sbjct: 121 ASRALAKMSFEDKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTIKKDVASGDEPSSS 180

Query: 181 -PTEAAEATDGVSEKPEAETVETPATDAEN 208
            P+EA +ATD    K E    E+ A +  N
Sbjct: 181 EPSEAVKATDSEVPKQEDAPQESAAVETVN 210

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BLAST of Spo12965.1 vs. UniProtKB/TrEMBL
Match: A0A058ZX73_EUCGR (Uncharacterized protein OS=Eucalyptus grandis GN=EUGRSUZ_K00245 PE=3 SV=1)

HSP 1 Score: 289.3 bits (739), Expect = 3.800e-75
Identity = 153/212 (72.17%), Postives = 168/212 (79.25%), Query Frame = 1


		  
Query: 1   MALELWGTLKEAITTYTGLSPTTFFTVVVLSLAFYHMVFGLFGSTPSPHQRHRSMEEEME 60
           MAL+LW TLKE+I  YTGLSP TFFTV+ L LA Y++   LFGS    HQR R  +E+M+
Sbjct: 1   MALQLWETLKESIVAYTGLSPATFFTVLALGLAVYYVASSLFGSPADHHQRPRDYQEQMQ 60

Query: 61  PLPPPVQLGEIEEDELKLYDGTDPKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDA 120
           PLPPPVQLGEI  +ELK YDG DPKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDA
Sbjct: 61  PLPPPVQLGEISGEELKQYDGADPKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDA 120

Query: 121 SRALAKMSFEDSDLTGDISGLGPFELDALQDWEYKFMSKYVKVGTIKSKDAPVTDVSSPT 180
           SRALAKMSFED DLTGDISGLGPFE+DALQDWEYKFMSKYVKVGTIK K  PVT+  S  
Sbjct: 121 SRALAKMSFEDKDLTGDISGLGPFEIDALQDWEYKFMSKYVKVGTIK-KTVPVTEGESTA 180

Query: 181 EAAE---------ATDGVSEKPEAETVETPAT 204
           E AE         + DG SE  EA+T ETP++
Sbjct: 181 EPAEVADKDVPKTSEDGPSESTEAKTEETPSS 211

[Close]

BLAST of Spo12965.1 vs. UniProtKB/TrEMBL
Match: B9R9D3_RICCO (Steroid binding protein, putative OS=Ricinus communis GN=RCOM_1496460 PE=3 SV=1)

HSP 1 Score: 287.3 bits (734), Expect = 1.500e-74
Identity = 154/219 (70.32%), Postives = 173/219 (79.00%), Query Frame = 1


		  
Query: 1   MALELWGTLKEAITTYTGLSPTTFFTVVVLSLAFYHMVFGLFGSTPSPHQRHRSMEEEME 60
           MAL+LW T KEAIT YTGLSP TFFTV+ L LA Y+++ G+FGS+ + HQR RS EE+M+
Sbjct: 3   MALQLWETFKEAITVYTGLSPATFFTVLALGLAVYYVISGMFGSSDTHHQRPRSFEEQMQ 62

Query: 61  PLPPPVQLGEIEEDELKLYDGTDPKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDA 120
           PLPPPVQLGE+ E+ELK YDG+DPKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDA
Sbjct: 63  PLPPPVQLGEVTEEELKQYDGSDPKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDA 122

Query: 121 SRALAKMSFEDSDLTGDISGLGPFELDALQDWEYKFMSKYVKVGTIKSKDAPVTD-VSSP 180
           SRALAKMSFED DLTGDISGLGPFEL+ALQDWEYKFMSKYVKVGTIK K  PVTD  +S 
Sbjct: 123 SRALAKMSFEDKDLTGDISGLGPFELEALQDWEYKFMSKYVKVGTIK-KPVPVTDEATSA 182

Query: 181 TEAAEATDGVSEKPE--------AETVETPATDAENKDE 211
           +E AE  +    KP         A+  E P+ D     E
Sbjct: 183 SEPAENKEVDDTKPAEDGPTADVAKPTEGPSADVAKSTE 220

[Close]

BLAST of Spo12965.1 vs. ExPASy Swiss-Prot
Match: MSBP2_ARATH (Membrane steroid-binding protein 2 OS=Arabidopsis thaliana GN=MSBP2 PE=1 SV=1)

HSP 1 Score: 261.5 bits (667), Expect = 7.700e-69
Identity = 135/191 (70.68%), Postives = 152/191 (79.58%), Query Frame = 1


		  
Query: 1   MALELWGTLKEAITTYTGLSPTTFFTVVVLSLAFYHMVFGLFGSTPSPHQRHRSMEEEME 60
           M  ++W TLKE IT YTGLSP  FFTV+ L+ A Y +V G F S      R   ++ + E
Sbjct: 1   MVQQIWETLKETITAYTGLSPAAFFTVLALAFAVYQVVSGFFVSPEVHRPRSLEVQPQSE 60

Query: 61  PLPPPVQLGEIEEDELKLYDGTDPKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDA 120
           PLPPPVQLGEI E+ELKLYDG+D KKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDA
Sbjct: 61  PLPPPVQLGEITEEELKLYDGSDSKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDA 120

Query: 121 SRALAKMSFEDSDLTGDISGLGPFELDALQDWEYKFMSKYVKVGTIKSKDAPVTDVSSPT 180
           SRALAKMSFED DLTGDISGLG FEL+ALQDWEYKFMSKYVKVGTI+ KD    + S P+
Sbjct: 121 SRALAKMSFEDQDLTGDISGLGAFELEALQDWEYKFMSKYVKVGTIQKKDGEGKESSEPS 180

Query: 181 EAAEAT-DGVS 191
           EA  A+ +G+S
Sbjct: 181 EAKTASAEGLS 191

[Close]

BLAST of Spo12965.1 vs. ExPASy Swiss-Prot
Match: MSBP1_ARATH (Membrane steroid-binding protein 1 OS=Arabidopsis thaliana GN=MSBP1 PE=1 SV=2)

HSP 1 Score: 246.9 bits (629), Expect = 2.000e-64
Identity = 138/220 (62.73%), Postives = 158/220 (71.82%), Query Frame = 1


		  
Query: 1   MALELWGTLKEAITTYTGLSPTTFFTVVVLSLAFYHMVFGLFGST---PSPHQRHRSMEE 60
           MALELW TLKEAI  YTGLSP  FFT + L+ A Y ++ G F S     + HQR RS+ +
Sbjct: 1   MALELWQTLKEAIHAYTGLSPVVFFTALALAFAIYQVISGWFASPFDDVNRHQRARSLAQ 60

Query: 61  EMEP-LPPPVQLGEIEEDELKLYDGTDPKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFA 120
           E EP +P PVQ+GEI E+ELK YDG+DP+KPLLMAIK QIYDV+QSRMFYGPGGPYALFA
Sbjct: 61  EEEPPIPQPVQVGEITEEELKQYDGSDPQKPLLMAIKHQIYDVTQSRMFYGPGGPYALFA 120

Query: 121 GKDASRALAKMSFEDSDLTGDISGLGPFELDALQDWEYKFMSKYVKVGTIK--SKDAPVT 180
           GKDASRALAKMSFE+ DLT D+SGLGPFELDALQDWEYKFMSKY KVGT+K    +    
Sbjct: 121 GKDASRALAKMSFEEKDLTWDVSGLGPFELDALQDWEYKFMSKYAKVGTVKVAGSEPETA 180

Query: 181 DVSSPTEAAEATDGVSEKPEAETV----ETPATDAENKDE 211
            VS PTE  E    V+  P    V    + PA     K+E
Sbjct: 181 SVSEPTENVEQDAHVTTTPGKTVVDKSDDAPAETVLKKEE 220

[Close]

BLAST of Spo12965.1 vs. ExPASy Swiss-Prot
Match: SBP3_ARATH (Probable steroid-binding protein 3 OS=Arabidopsis thaliana GN=MP3 PE=1 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 5.400e-22
Identity = 48/99 (48.48%), Postives = 68/99 (68.69%), Query Frame = 1


		  
Query: 70  EIEEDELKLYDGTDPKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDASRALAKMSF 129
           E   ++L  Y+GTD  KP+ +AIKG+++DV+  + FYG GG Y++FAGKDASRAL KMS 
Sbjct: 2   EFTAEQLSQYNGTDESKPIYVAIKGRVFDVTTGKSFYGSGGDYSMFAGKDASRALGKMSK 61

Query: 130 EDSDLTGDISGLGPFELDALQDWEYKFMSKYVKVGTIKS 169
            + D++  + GL   E++ L DWE KF +KY  VG + S
Sbjct: 62  NEEDVSPSLEGLTEKEINTLNDWETKFEAKYPVVGRVVS 100

[Close]

BLAST of Spo12965.1 vs. ExPASy Swiss-Prot
Match: NENF_RAT (Neudesin OS=Rattus norvegicus GN=Nenf PE=2 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 3.600e-18
Identity = 55/138 (39.86%), Postives = 78/138 (56.52%), Query Frame = 1


		  
Query: 28  VVLSLAFYHMVFGLFGSTPSPHQRHRSMEEEMEPLPPPVQLGEIEEDELKLYDGTDPKKP 87
           + L+LA   +     G  P P +R            PPV+L    E+EL  Y G +  +P
Sbjct: 16  LALALALVRVPSARAGQMPRPAERG-----------PPVRL--FTEEELARYSGEEEDQP 75

Query: 88  LLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDASRALAKMSFEDSDLTGDISGLGPFELD 147
           + +A+KG ++DV+  + FYG G PY   AGKD+SR +AKMS + +DLT DISGL   EL+
Sbjct: 76  IYLAVKGVVFDVTSGKEFYGRGAPYNALAGKDSSRGVAKMSLDPADLTHDISGLTAKELE 135

Query: 148 ALQD-WEYKFMSKYVKVG 165
           AL D +   + +KY  VG
Sbjct: 136 ALDDIFSKVYKAKYPIVG 140

[Close]

BLAST of Spo12965.1 vs. ExPASy Swiss-Prot
Match: NENF_MOUSE (Neudesin OS=Mus musculus GN=Nenf PE=1 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 4.700e-18
Identity = 55/138 (39.86%), Postives = 79/138 (57.25%), Query Frame = 1


		  
Query: 28  VVLSLAFYHMVFGLFGSTPSPHQRHRSMEEEMEPLPPPVQLGEIEEDELKLYDGTDPKKP 87
           +VL+LA   +     G TP P +R            PPV+L    E+EL  Y G +  +P
Sbjct: 16  LVLALALVPVPSAWAGQTPRPAERG-----------PPVRL--FTEEELARYGGEEEDQP 75

Query: 88  LLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDASRALAKMSFEDSDLTGDISGLGPFELD 147
           + +A+KG ++DV+  + FYG G PY   AGKD+SR +AKMS + +DLT D +GL   EL+
Sbjct: 76  IYLAVKGVVFDVTSGKEFYGRGAPYNALAGKDSSRGVAKMSLDPADLTHDTTGLTAKELE 135

Query: 148 ALQD-WEYKFMSKYVKVG 165
           AL D +   + +KY  VG
Sbjct: 136 ALDDVFSKVYKAKYPIVG 140

[Close]

BLAST of Spo12965.1 vs. TAIR (Arabidopsis)
Match: AT3G48890.1 (membrane-associated progesterone binding protein 3)

HSP 1 Score: 261.5 bits (667), Expect = 4.300e-70
Identity = 135/191 (70.68%), Postives = 152/191 (79.58%), Query Frame = 1


		  
Query: 1   MALELWGTLKEAITTYTGLSPTTFFTVVVLSLAFYHMVFGLFGSTPSPHQRHRSMEEEME 60
           M  ++W TLKE IT YTGLSP  FFTV+ L+ A Y +V G F S      R   ++ + E
Sbjct: 1   MVQQIWETLKETITAYTGLSPAAFFTVLALAFAVYQVVSGFFVSPEVHRPRSLEVQPQSE 60

Query: 61  PLPPPVQLGEIEEDELKLYDGTDPKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDA 120
           PLPPPVQLGEI E+ELKLYDG+D KKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDA
Sbjct: 61  PLPPPVQLGEITEEELKLYDGSDSKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDA 120

Query: 121 SRALAKMSFEDSDLTGDISGLGPFELDALQDWEYKFMSKYVKVGTIKSKDAPVTDVSSPT 180
           SRALAKMSFED DLTGDISGLG FEL+ALQDWEYKFMSKYVKVGTI+ KD    + S P+
Sbjct: 121 SRALAKMSFEDQDLTGDISGLGAFELEALQDWEYKFMSKYVKVGTIQKKDGEGKESSEPS 180

Query: 181 EAAEAT-DGVS 191
           EA  A+ +G+S
Sbjct: 181 EAKTASAEGLS 191

[Close]

BLAST of Spo12965.1 vs. TAIR (Arabidopsis)
Match: AT5G52240.1 (membrane steroid binding protein 1)

HSP 1 Score: 246.9 bits (629), Expect = 1.100e-65
Identity = 138/220 (62.73%), Postives = 158/220 (71.82%), Query Frame = 1


		  
Query: 1   MALELWGTLKEAITTYTGLSPTTFFTVVVLSLAFYHMVFGLFGST---PSPHQRHRSMEE 60
           MALELW TLKEAI  YTGLSP  FFT + L+ A Y ++ G F S     + HQR RS+ +
Sbjct: 1   MALELWQTLKEAIHAYTGLSPVVFFTALALAFAIYQVISGWFASPFDDVNRHQRARSLAQ 60

Query: 61  EMEP-LPPPVQLGEIEEDELKLYDGTDPKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFA 120
           E EP +P PVQ+GEI E+ELK YDG+DP+KPLLMAIK QIYDV+QSRMFYGPGGPYALFA
Sbjct: 61  EEEPPIPQPVQVGEITEEELKQYDGSDPQKPLLMAIKHQIYDVTQSRMFYGPGGPYALFA 120

Query: 121 GKDASRALAKMSFEDSDLTGDISGLGPFELDALQDWEYKFMSKYVKVGTIK--SKDAPVT 180
           GKDASRALAKMSFE+ DLT D+SGLGPFELDALQDWEYKFMSKY KVGT+K    +    
Sbjct: 121 GKDASRALAKMSFEEKDLTWDVSGLGPFELDALQDWEYKFMSKYAKVGTVKVAGSEPETA 180

Query: 181 DVSSPTEAAEATDGVSEKPEAETV----ETPATDAENKDE 211
            VS PTE  E    V+  P    V    + PA     K+E
Sbjct: 181 SVSEPTENVEQDAHVTTTPGKTVVDKSDDAPAETVLKKEE 220

[Close]

BLAST of Spo12965.1 vs. TAIR (Arabidopsis)
Match: AT2G24940.1 (membrane-associated progesterone binding protein 2)

HSP 1 Score: 105.9 bits (263), Expect = 3.000e-23
Identity = 48/99 (48.48%), Postives = 68/99 (68.69%), Query Frame = 1


		  
Query: 70  EIEEDELKLYDGTDPKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDASRALAKMSF 129
           E   ++L  Y+GTD  KP+ +AIKG+++DV+  + FYG GG Y++FAGKDASRAL KMS 
Sbjct: 2   EFTAEQLSQYNGTDESKPIYVAIKGRVFDVTTGKSFYGSGGDYSMFAGKDASRALGKMSK 61

Query: 130 EDSDLTGDISGLGPFELDALQDWEYKFMSKYVKVGTIKS 169
            + D++  + GL   E++ L DWE KF +KY  VG + S
Sbjct: 62  NEEDVSPSLEGLTEKEINTLNDWETKFEAKYPVVGRVVS 100

[Close]

BLAST of Spo12965.1 vs. TAIR (Arabidopsis)
Match: AT4G14965.1 (membrane-associated progesterone binding protein 4)

HSP 1 Score: 81.3 bits (199), Expect = 8.000e-16
Identity = 37/93 (39.78%), Postives = 54/93 (58.06%), Query Frame = 1


		  
Query: 74  DELKLYDGTDPKKPLLMAIKGQIYDVSQSRMFYGPGGPYALFAGKDASRALAKMSFEDSD 133
           +EL LY+GTD   P+L+ I G ++DV++ +  YG GG Y  FAG+DASRA    +F    
Sbjct: 45  EELALYNGTDETLPILLGILGSVFDVTKGKFHYGSGGGYNHFAGRDASRAFVSGNFTGDG 104

Query: 134 LTGDISGLGPFELDALQDWEYKFMSKYVKVGTI 167
           LT  + GL   E+ ++ DW   +   Y  VG +
Sbjct: 105 LTDSLQGLSSSEVKSIVDWRGFYSRTYTPVGKL 137

The following BLAST results are available for this feature:

BLAST of Spo12965.1 vs. NCBI nr
BLAST of Spo12965.1 vs. UniProtKB/TrEMBL
BLAST of Spo12965.1 vs. ExPASy Swiss-Prot
BLAST of Spo12965.1 vs. TAIR (Arabidopsis)

BLAST of Spo12965.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902235440\|gb\|KNA23910.1\|	1.3e-111	99.5	hypothetical protein SOVF_0195...	[more]
gi\|731363620\|ref\|XP_010693524.1\|	2.2e-84	74.8	PREDICTED: membrane steroid-bi...	[more]
gi\|702488501\|ref\|XP_010035120.1\|	5.5e-75	72.1	PREDICTED: membrane steroid-bi...	[more]
gi\|743857017\|ref\|XP_011030067.1\|	1.2e-74	72.3	PREDICTED: membrane steroid-bi...	[more]
gi\|255539487\|ref\|XP_002510808.1\|	2.1e-74	70.3	PREDICTED: membrane steroid-bi...	[more]

BLAST of Spo12965.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9RWJ8_SPIOL	8.8e-112	99.5	Uncharacterized protein OS=Spi...	[more]
A0A0J8BDV2_BETVU	1.6e-84	74.8	Uncharacterized protein OS=Bet...	[more]
G5DXK7_SILLA	1.7e-78	77.6	Membrane steroid-binding prote...	[more]
A0A058ZX73_EUCGR	3.8e-75	72.1	Uncharacterized protein OS=Euc...	[more]
B9R9D3_RICCO	1.5e-74	70.3	Steroid binding protein, putat...	[more]

BLAST of Spo12965.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
MSBP2_ARATH	7.7e-69	70.6	Membrane steroid-binding prote...	[more]
MSBP1_ARATH	2.0e-64	62.7	Membrane steroid-binding prote...	[more]
SBP3_ARATH	5.4e-22	48.4	Probable steroid-binding prote...	[more]
NENF_RAT	3.6e-18	39.8	Neudesin OS=Rattus norvegicus ...	[more]
NENF_MOUSE	4.7e-18	39.8	Neudesin OS=Mus musculus GN=Ne...	[more]

BLAST of Spo12965.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 4


Match Name	E-value	Identity	Description
AT3G48890.1	4.3e-70	70.6	membrane-associated progestero...	[more]
AT5G52240.1	1.1e-65	62.7	membrane steroid binding prote...	[more]
AT2G24940.1	3.0e-23	48.4	membrane-associated progestero...	[more]
AT4G14965.1	8.0e-16	39.7	membrane-associated progestero...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001199	Cytochrome b5-like heme/steroid binding domain	GENE3D	3.10.120.10		coord: 71..166 score: 3.2
IPR001199	Cytochrome b5-like heme/steroid binding domain	PFAM	PF00173	Cyt-b5	coord: 73..166 score: 5.5
IPR001199	Cytochrome b5-like heme/steroid binding domain	SMART	SM01117	Cyt_b5_2	coord: 71..167 score: 2.8
IPR001199	Cytochrome b5-like heme/steroid binding domain	unknown	SSF55856	Cytochrome b5-like heme/steroid binding domain	coord: 63..167 score: 3.73
None	No IPR available	PANTHER	PTHR10281	MEMBRANE-ASSOCIATED PROGESTERONE RECEPTOR COMPONENT-RELATED	coord: 1..184 score: 3.7E
None	No IPR available	PANTHER	PTHR10281:SF45	MEMBRANE STEROID-BINDING PROTEIN 1-RELATED	coord: 1..184 score: 3.7E

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055114	oxidation-reduction process
molecular_function	GO:0032440	2-alkenal reductase [NAD(P)] activity
molecular_function	GO:0020037	heme binding