Spo13171.1 (mRNA) | SpinachBase

Overview


Name	Spo13171.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	Methyltransferase-related protein, putative
Location	chr3 : 10283158 .. 10284208 (+)
Sequence length	953

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TCCAATATTCTTTCTGCAACAAAGGTATTTCAATTATTTTTCTCTCTCTAATAATCTTGCCGGAATTACTTAGGCAGAAGGGTGGGAAATTTGAAAATAACCCAGAAATTGTTTGTATATTTCTAAAAAATGTGCCCTTTAAGATTCATTTTAGTGTTTTTCTCTGCAATTTTGGCTGGATTTTTGGCATGGAAATCATTTCGTTCTGCACCCGAGCTCGATACCGCCTTTTCTGAAGATTTGGTGGCAGATAAACCAGCGTCGTCAATGGATGATCACCAAGAAAAGAACCTTAAAATGGTAAATATCCTATTCCTTTTTATTTTCTTTTTTTGGGATTTTTTTTCTGTAATTTGTTTTCTAAAGTACATTTTATTTACGTTTTTGGAAATTTCCAGATAATTCAAGACGGTTTCTGGGTATTTGTTGACATGGCTAGTGGGAGATACTTGTGGAGAAATTTAAAGGGGATGAAGACAGTGGAGAAAGTAAAGAACTGCTAAAATCTCAATTTCAGAATCCTTGCATTTTTCACTTTGTTGAATATGAGTTACAGATTACATCTTTCTCTTCTTTTTCTTTTTTGTGTAAAAGGAACCGCGATTACATAGACAGGATTCGATCAAACGTCATTGATATGAGTACGAGTTACTTTGTAAGTTGCCAAAGTGTGTAAATTTGTTATTCTGACTTTAAGAATCTGGGAAAATTTTCTATGTCGAATAACTTTGTACTACTTGCAATTGTGCATGATTTCTGCTAAAGAAATTTATACCCAGTTTAACTTTTAATTGCAAAATGTGACTATATATCTTGGGCTGAATTTTTGGCTATTTTGTTATCCGTTAATGGTTCAACTTAAAGTCAACCGACACTTTGGAAAGATTGTTTAATCATCCTCTTGCAGCTTTACAGCAGAGAAGCAGAATATTACTCAATCCTGTAATGTATAAGTTCTGATTTGCTCAAAACGTGACTGCAATGAATATACTTTTTTTTTCCAATTAAGGTAAGGAAAAGATAAAACAAGCAATGCCTAGATACACACACA

mRNA sequence

TCCAATATTCTTTCTGCAACAAAGGTATTTCAATTATTTTTCTCTCTCTAATAATCTTGCCGGAATTACTTAGGCAGAAGGGTGGGAAATTTGAAAATAACCCAGAAATTGTTTGTATATTTCTAAAAAATGTGCCCTTTAAGATTCATTTTAGTGTTTTTCTCTGCAATTTTGGCTGGATTTTTGGCATGGAAATCATTTCGTTCTGCACCCGAGCTCGATACCGCCTTTTCTGAAGATTTGGTGGCAGATAAACCAGCGTCGTCAATGGATGATCACCAAGAAAAGAACCTTAAAATGATAATTCAAGACGGTTTCTGGGTATTTGTTGACATGGCTAGTGGGAGATACTTGTGGAGAAATTTAAAGGGGATGAAGACAGTGGAGAAAGTAAAGAACTGCTAAAATCTCAATTTCAGAATCCTTGCATTTTTCACTTTGTTGAATATGAGTTACAGATTACATCTTTCTCTTCTTTTTCTTTTTTGTGTAAAAGGAACCGCGATTACATAGACAGGATTCGATCAAACGTCATTGATATGAGTACGAGTTACTTTGTAAGTTGCCAAAGTGTGTAAATTTGTTATTCTGACTTTAAGAATCTGGGAAAATTTTCTATGTCGAATAACTTTGTACTACTTGCAATTGTGCATGATTTCTGCTAAAGAAATTTATACCCAGTTTAACTTTTAATTGCAAAATGTGACTATATATCTTGGGCTGAATTTTTGGCTATTTTGTTATCCGTTAATGGTTCAACTTAAAGTCAACCGACACTTTGGAAAGATTGTTTAATCATCCTCTTGCAGCTTTACAGCAGAGAAGCAGAATATTACTCAATCCTGTAATGTATAAGTTCTGATTTGCTCAAAACGTGACTGCAATGAATATACTTTTTTTTTCCAATTAAGGTAAGGAAAAGATAAAACAAGCAATGCCTAGATACACACACA

Coding sequence (CDS)

ATGTGCCCTTTAAGATTCATTTTAGTGTTTTTCTCTGCAATTTTGGCTGGATTTTTGGCATGGAAATCATTTCGTTCTGCACCCGAGCTCGATACCGCCTTTTCTGAAGATTTGGTGGCAGATAAACCAGCGTCGTCAATGGATGATCACCAAGAAAAGAACCTTAAAATGATAATTCAAGACGGTTTCTGGGTATTTGTTGACATGGCTAGTGGGAGATACTTGTGGAGAAATTTAAAGGGGATGAAGACAGTGGAGAAAGTAAAGAACTGCTAA

Protein sequence

MCPLRFILVFFSAILAGFLAWKSFRSAPELDTAFSEDLVADKPASSMDDHQEKNLKMIIQDGFWVFVDMASGRYLWRNLKGMKTVEKVKNC

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo13171	Spo13171	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo13171.1	Spo13171.1-protein	polypeptide

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo13171.1.exon.1	Spo13171.1.exon.1	exon
Spo13171.1.exon.2	Spo13171.1.exon.2	exon

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo13171.1.utr5p.1	Spo13171.1.utr5p.1	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo13171.1.CDS.1	Spo13171.1.CDS.1	CDS
Spo13171.1.CDS.2	Spo13171.1.CDS.2	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo13171.1.utr3p.1	Spo13171.1.utr3p.1	three_prime_UTR

Homology

[Close]

BLAST of Spo13171.1 vs. NCBI nr
Match: gi|902217337|gb|KNA17665.1| (hypothetical protein SOVF_077870 [Spinacia oleracea])

HSP 1 Score: 187.6 bits (475), Expect = 9.800e-45
Identity = 91/91 (100.00%), Postives = 91/91 (100.00%), Query Frame = 1


		  
Query: 1  MCPLRFILVFFSAILAGFLAWKSFRSAPELDTAFSEDLVADKPASSMDDHQEKNLKMIIQ 60
          MCPLRFILVFFSAILAGFLAWKSFRSAPELDTAFSEDLVADKPASSMDDHQEKNLKMIIQ
Sbjct: 1  MCPLRFILVFFSAILAGFLAWKSFRSAPELDTAFSEDLVADKPASSMDDHQEKNLKMIIQ 60

Query: 61 DGFWVFVDMASGRYLWRNLKGMKTVEKVKNC 92
          DGFWVFVDMASGRYLWRNLKGMKTVEKVKNC
Sbjct: 61 DGFWVFVDMASGRYLWRNLKGMKTVEKVKNC 91

[Close]

BLAST of Spo13171.1 vs. NCBI nr
Match: gi|731322708|ref|XP_010672044.1| (PREDICTED: uncharacterized protein LOC104888707 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 155.2 bits (391), Expect = 5.400e-35
Identity = 75/91 (82.42%), Postives = 80/91 (87.91%), Query Frame = 1


		  
Query: 1  MCPLRFILVFFSAILAGFLAWKSFRSAPELDTAFSEDLVADKPASSMDDHQEKNLKMIIQ 60
          MCPLRFILVFFSAILAGFLAW+SFRS+PELD A SED  ADK  SS DD QE+NLKMIIQ
Sbjct: 1  MCPLRFILVFFSAILAGFLAWRSFRSSPELDFAISEDSTADKTTSSKDDKQERNLKMIIQ 60

Query: 61 DGFWVFVDMASGRYLWRNLKGMKTVEKVKNC 92
          DGFWVFVDMASG+YLWRN K M TVEKVK+C
Sbjct: 61 DGFWVFVDMASGKYLWRNFKEMNTVEKVKSC 91

[Close]

BLAST of Spo13171.1 vs. NCBI nr
Match: gi|743821683|ref|XP_011021479.1| (PREDICTED: uncharacterized protein LOC105123549 [Populus euphratica])

HSP 1 Score: 117.1 bits (292), Expect = 1.600e-23
Identity = 57/90 (63.33%), Postives = 72/90 (80.00%), Query Frame = 1


		  
Query: 1  MCPLRFILVFFSAILAGFLAWKSFRSAPELDTAFSEDLVADKPASSMDDHQEKNLKMIIQ 60
          MCPLRFILVFFSA+LAG+ AW++ RS+PE+D   S+D  A+K  +S++D QE NLK +IQ
Sbjct: 1  MCPLRFILVFFSAVLAGYFAWRTVRSSPEIDDMVSDDSTAEK--TSLEDKQEFNLKRMIQ 60

Query: 61 DGFWVFVDMASGRYLWRNLKGMKTVEKVKN 91
          +GFWVFVDMASG YLWRNLK MK    +K+
Sbjct: 61 NGFWVFVDMASGSYLWRNLKEMKKDATLKS 88

[Close]

BLAST of Spo13171.1 vs. NCBI nr
Match: gi|224125870|ref|XP_002319695.1| (hypothetical protein POPTR_0013s05470g [Populus trichocarpa])

HSP 1 Score: 116.3 bits (290), Expect = 2.800e-23
Identity = 57/90 (63.33%), Postives = 71/90 (78.89%), Query Frame = 1


		  
Query: 1  MCPLRFILVFFSAILAGFLAWKSFRSAPELDTAFSEDLVADKPASSMDDHQEKNLKMIIQ 60
          MCPLRFILVFFSA+LAG+ AW++ RS+PE+D   S+D  A+K  +S+ + QE N K IIQ
Sbjct: 1  MCPLRFILVFFSAVLAGYFAWRTVRSSPEIDDFVSDDSTAEK--TSLREKQEFNFKRIIQ 60

Query: 61 DGFWVFVDMASGRYLWRNLKGMKTVEKVKN 91
          +GFWVFVDMASGRYLWRN K MK  E +K+
Sbjct: 61 NGFWVFVDMASGRYLWRNFKEMKKDETLKS 88

[Close]

BLAST of Spo13171.1 vs. NCBI nr
Match: gi|743873352|ref|XP_011034377.1| (PREDICTED: uncharacterized protein LOC105132516 [Populus euphratica])

HSP 1 Score: 115.5 bits (288), Expect = 4.700e-23
Identity = 57/90 (63.33%), Postives = 70/90 (77.78%), Query Frame = 1


		  
Query: 1  MCPLRFILVFFSAILAGFLAWKSFRSAPELDTAFSEDLVADKPASSMDDHQEKNLKMIIQ 60
          MCPLRFILVFFSA+LAG+ AW++ RS+PE+D   S+D  A+K  + + D QE N K IIQ
Sbjct: 1  MCPLRFILVFFSAVLAGYFAWRTVRSSPEIDDFVSDDSTAEK--TYLRDKQEFNFKRIIQ 60

Query: 61 DGFWVFVDMASGRYLWRNLKGMKTVEKVKN 91
          +GFWVFVDMASGRYLWRN K MK  E +K+
Sbjct: 61 NGFWVFVDMASGRYLWRNFKEMKKDETLKS 88

[Close]

BLAST of Spo13171.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RDV6_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_077870 PE=4 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 6.800e-45
Identity = 91/91 (100.00%), Postives = 91/91 (100.00%), Query Frame = 1


		  
Query: 1  MCPLRFILVFFSAILAGFLAWKSFRSAPELDTAFSEDLVADKPASSMDDHQEKNLKMIIQ 60
          MCPLRFILVFFSAILAGFLAWKSFRSAPELDTAFSEDLVADKPASSMDDHQEKNLKMIIQ
Sbjct: 1  MCPLRFILVFFSAILAGFLAWKSFRSAPELDTAFSEDLVADKPASSMDDHQEKNLKMIIQ 60

Query: 61 DGFWVFVDMASGRYLWRNLKGMKTVEKVKNC 92
          DGFWVFVDMASGRYLWRNLKGMKTVEKVKNC
Sbjct: 61 DGFWVFVDMASGRYLWRNLKGMKTVEKVKNC 91

[Close]

BLAST of Spo13171.1 vs. UniProtKB/TrEMBL
Match: A0A0J8CUK9_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_3g056150 PE=4 SV=1)

HSP 1 Score: 155.2 bits (391), Expect = 3.800e-35
Identity = 75/91 (82.42%), Postives = 80/91 (87.91%), Query Frame = 1


		  
Query: 1  MCPLRFILVFFSAILAGFLAWKSFRSAPELDTAFSEDLVADKPASSMDDHQEKNLKMIIQ 60
          MCPLRFILVFFSAILAGFLAW+SFRS+PELD A SED  ADK  SS DD QE+NLKMIIQ
Sbjct: 1  MCPLRFILVFFSAILAGFLAWRSFRSSPELDFAISEDSTADKTTSSKDDKQERNLKMIIQ 60

Query: 61 DGFWVFVDMASGRYLWRNLKGMKTVEKVKNC 92
          DGFWVFVDMASG+YLWRN K M TVEKVK+C
Sbjct: 61 DGFWVFVDMASGKYLWRNFKEMNTVEKVKSC 91

[Close]

BLAST of Spo13171.1 vs. UniProtKB/TrEMBL
Match: B9I5Y1_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0013s05470g PE=4 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 1.900e-23
Identity = 57/90 (63.33%), Postives = 71/90 (78.89%), Query Frame = 1


		  
Query: 1  MCPLRFILVFFSAILAGFLAWKSFRSAPELDTAFSEDLVADKPASSMDDHQEKNLKMIIQ 60
          MCPLRFILVFFSA+LAG+ AW++ RS+PE+D   S+D  A+K  +S+ + QE N K IIQ
Sbjct: 1  MCPLRFILVFFSAVLAGYFAWRTVRSSPEIDDFVSDDSTAEK--TSLREKQEFNFKRIIQ 60

Query: 61 DGFWVFVDMASGRYLWRNLKGMKTVEKVKN 91
          +GFWVFVDMASGRYLWRN K MK  E +K+
Sbjct: 61 NGFWVFVDMASGRYLWRNFKEMKKDETLKS 88

[Close]

BLAST of Spo13171.1 vs. UniProtKB/TrEMBL
Match: B9SHF7_RICCO (Putative uncharacterized protein OS=Ricinus communis GN=RCOM_1121640 PE=4 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 7.300e-23
Identity = 55/92 (59.78%), Postives = 71/92 (77.17%), Query Frame = 1


		  
Query: 1  MCPLRFILVFFSAILAGFLAWKSFRSAPELDTAFSEDLVADKPASSMDDHQEK--NLKMI 60
          MCPLRFILVFFSA+LAG+ AW++ RS+PE++T  S+D   +K     D++  K  + K I
Sbjct: 1  MCPLRFILVFFSAVLAGYFAWRTVRSSPEIETTVSDDSTVEKKPLQDDNNNNKVCSAKRI 60

Query: 61 IQDGFWVFVDMASGRYLWRNLKGMKTVEKVKN 91
          IQ+GFWVFVDMASG+YLWRNLK M+  EKVK+
Sbjct: 61 IQNGFWVFVDMASGKYLWRNLKEMREDEKVKS 92

[Close]

BLAST of Spo13171.1 vs. UniProtKB/TrEMBL
Match: A0A061F3J3_THECC (Methyltransferase-related protein, putative OS=Theobroma cacao GN=TCM_026468 PE=4 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 1.600e-22
Identity = 55/90 (61.11%), Postives = 72/90 (80.00%), Query Frame = 1


		  
Query: 1  MCPLRFILVFFSAILAGFLAWKSFRSAPELDTAFSEDLVADKPASSMDDHQEKNLKMIIQ 60
          MCP+RF+LVFFSA+LAG++AW++ RS+ ++D   SED  +DK  +   D QE + + ++Q
Sbjct: 1  MCPMRFLLVFFSAVLAGYVAWRTVRSSSDIDDVVSED--SDKMVAK--DKQEFSFRRMVQ 60

Query: 61 DGFWVFVDMASGRYLWRNLKGMKTVEKVKN 91
          +GFWVFVDMASGRYLWRNLK MKT EKVKN
Sbjct: 61 NGFWVFVDMASGRYLWRNLKEMKTDEKVKN 86

[Close]

BLAST of Spo13171.1 vs. TAIR (Arabidopsis)
Match: AT5G18150.1 (Methyltransferase-related protein)

HSP 1 Score: 97.1 bits (240), Expect = 6.100e-21
Identity = 46/83 (55.42%), Postives = 60/83 (72.29%), Query Frame = 1


		  
Query: 1  MCPLRFILVFFSAILAGFLAWKSFRSAPELDTAFSEDLVADKPASSMDDHQEKNLKMIIQ 60
          MCP+RF+LVFFSA+LAG+ AWK+  S+PE D+              +++ QE NLK  ++
Sbjct: 1  MCPMRFLLVFFSAVLAGYFAWKTVSSSPEFDSP-----------DELNEKQELNLKKKME 60

Query: 61 DGFWVFVDMASGRYLWRNLKGMK 84
          +GFWVFVDMASGRYLWRNLK M+
Sbjct: 61 NGFWVFVDMASGRYLWRNLKEMR 72

[Close]

BLAST of Spo13171.1 vs. TAIR (Arabidopsis)
Match: AT5G14602.1 (FUNCTIONS IN: molecular_function unknown)

HSP 1 Score: 89.0 bits (219), Expect = 1.700e-18
Identity = 44/83 (53.01%), Postives = 59/83 (71.08%), Query Frame = 1


		  
Query: 1  MCPLRFILVFFSAILAGFLAWKSFRSAPELDTAFSEDLVADKPASSMDDHQEKNLKMIIQ 60
          MCPLRF+LVFFSA+LAG++AW++  S PEL   FS++L+     +   + Q  + K  + 
Sbjct: 1  MCPLRFVLVFFSAVLAGYIAWRTVNSTPEL---FSDELL----QAEAKEKQGLDFKRKMD 60

Query: 61 DGFWVFVDMASGRYLWRNLKGMK 84
          +G W FVDMASGRYLWRNLK M+
Sbjct: 61 NGLWTFVDMASGRYLWRNLKEMR 76

[Close]

BLAST of Spo13171.1 vs. TAIR (Arabidopsis)
Match: AT5G58375.1 (Methyltransferase-related protein)

HSP 1 Score: 63.9 bits (154), Expect = 5.800e-11
Identity = 37/81 (45.68%), Postives = 47/81 (58.02%), Query Frame = 1


		  
Query: 1  MCPLRFILVFFSAILAGFLAWKSFRSAPE--LDTAFSEDLVADKPASSMDDHQEKNLKMI 60
          MCPLR IL+F SA LAGF   +   S  +  LD +F++   AD  + S        + M 
Sbjct: 1  MCPLRLILIFLSATLAGFFVLQKLNSTSDDPLDDSFTDAEHADDDSVS----GFSKVGMA 60

Query: 61 IQDGFWVFVDMASGRYLWRNL 80
          ++ GFW  VDMASGRYLW NL
Sbjct: 61 MKSGFWTCVDMASGRYLWNNL 77

The following BLAST results are available for this feature:

BLAST of Spo13171.1 vs. NCBI nr
BLAST of Spo13171.1 vs. UniProtKB/TrEMBL
BLAST of Spo13171.1 vs. ExPASy Swiss-Prot
BLAST of Spo13171.1 vs. TAIR (Arabidopsis)

BLAST of Spo13171.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902217337\|gb\|KNA17665.1\|	9.8e-45	100.	hypothetical protein SOVF_0778...	[more]
gi\|731322708\|ref\|XP_010672044.1\|	5.4e-35	82.4	PREDICTED: uncharacterized pro...	[more]
gi\|743821683\|ref\|XP_011021479.1\|	1.6e-23	63.3	PREDICTED: uncharacterized pro...	[more]
gi\|224125870\|ref\|XP_002319695.1\|	2.8e-23	63.3	hypothetical protein POPTR_001...	[more]
gi\|743873352\|ref\|XP_011034377.1\|	4.7e-23	63.3	PREDICTED: uncharacterized pro...	[more]

BLAST of Spo13171.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9RDV6_SPIOL	6.8e-45	100.	Uncharacterized protein OS=Spi...	[more]
A0A0J8CUK9_BETVU	3.8e-35	82.4	Uncharacterized protein OS=Bet...	[more]
B9I5Y1_POPTR	1.9e-23	63.3	Uncharacterized protein OS=Pop...	[more]
B9SHF7_RICCO	7.3e-23	59.7	Putative uncharacterized prote...	[more]
A0A061F3J3_THECC	1.6e-22	61.1	Methyltransferase-related prot...	[more]

BLAST of Spo13171.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Spo13171.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 3


Match Name	E-value	Identity	Description
AT5G18150.1	6.1e-21	55.4	Methyltransferase-related prot...	[more]
AT5G14602.1	1.7e-18	53.0	FUNCTIONS IN: molecular_functi...	[more]
AT5G58375.1	5.8e-11	45.6	Methyltransferase-related prot...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR34132	FAMILY NOT NAMED	coord: 2..90 score: 7.1

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010200	response to chitin
biological_process	GO:0030488	tRNA methylation
cellular_component	GO:0005634	nucleus
cellular_component	GO:0031515	tRNA (m1A) methyltransferase complex
molecular_function	GO:0016429	tRNA (adenine-N1-)-methyltransferase activity