Spo13623.1 (mRNA)

BLAST of Spo13623.1 vs. NCBI nr
Match: gi|731319208|ref|XP_010670137.1| (PREDICTED: valine--tRNA ligase, mitochondrial isoform X3 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 851.7 bits (2199), Expect = 9.400e-244
Identity = 459/608 (75.49%), Postives = 496/608 (81.58%), Query Frame = 1

		  

Query: 84  GLDRFEAREKLWKDLTETGLAVKKQPHTLRVPRSQRGGEVRIIEPLVSKQWFVTMEALAE 143
           GLDRFEAREKLW DL ETGLAVKK+PHTLRVPRSQRGGE  IIEPLVSKQWFVTME LAE
Sbjct: 251 GLDRFEAREKLWNDLLETGLAVKKEPHTLRVPRSQRGGE--IIEPLVSKQWFVTMEPLAE 310

Query: 144 KALHAVEKGDLTIIPDRFEKIYNHWLTNIKDWCISRQLWWGHRIPVWYIVGRDCEEEYIV 203
           KALHAVE GDLTI+PDRFEKIYNHWL+NIKDWCISRQLWWGHRIPVWYIVG+DCEEEYIV
Sbjct: 311 KALHAVETGDLTILPDRFEKIYNHWLSNIKDWCISRQLWWGHRIPVWYIVGKDCEEEYIV 370

Query: 204 ARSQEEALHKAQQKYGKDAEIYQDPDVLDTWFSSALWPFSTLGWPDVSQEEYKMFYPTTM 263
           ARS EEALHKAQQKYGKDAEIYQDPDVLDTWFSS+LWPFSTLGWPDVS +++K FYPTTM
Sbjct: 371 ARSSEEALHKAQQKYGKDAEIYQDPDVLDTWFSSSLWPFSTLGWPDVSDDDFKSFYPTTM 430

Query: 264 LETGHDILFFWVARMVMMGIEFTGNVPFRYVYLHGLIRDSQGRKMSKTLGNVIDPVDTIK 323
           LETGHDILFFWVARMVMMGIEFTGNVPFRYVYLHGLIRDSQGRKMSKTLGNVIDPVDTIK
Sbjct: 431 LETGHDILFFWVARMVMMGIEFTGNVPFRYVYLHGLIRDSQGRKMSKTLGNVIDPVDTIK 490

Query: 324 DFGTDALRFTLALGTAGQFDKEESVLSLRLPECWVVSKLHLLIDLVTTSYDKFYFGDVAR 383
           +FGTDALRFTLALGTAGQ D   S   L   + +  +KL         +  KF   ++  
Sbjct: 491 EFGTDALRFTLALGTAGQ-DLNLSAERLSANKAF-TNKL--------WNAGKFMLQNLPS 550

Query: 384 ETYDFFWGDFADW-YAYSNSLCAQILLNLKLQQQLYLLSILMKVS------------LFD 443
           ++  F W     + +   +SL    L    +  +L+ L  L+  S             +D
Sbjct: 551 QSDVFAWETLRAYEFDKEDSLLKLRLPECWVVSKLHGLIDLVTASYDKFYFGDVARETYD 610

Query: 444 FFDMNACLIRYIEASKARLYQSEDNSVTTAAQAVLLYVFENILKILHPFMPFVTEALWQA 503
           FF        YIEASKARLYQS D SVT+AAQAVLLYVFENILKILHPFMP+VTEALWQA
Sbjct: 611 FF-WGDFADWYIEASKARLYQSGDKSVTSAAQAVLLYVFENILKILHPFMPYVTEALWQA 670

Query: 504 IPNQKEPLIVSSWPKTSLPSYSNA----------TRAIRNVRAEYSVEPVKRISATIVAN 563
           IP QK PLIVSSWP TSLP YSNA          TRAIRN RAEYSVEPVKRI+AT+VAN
Sbjct: 671 IPYQKAPLIVSSWPTTSLPVYSNAIKRFENFQALTRAIRNARAEYSVEPVKRIAATVVAN 730

Query: 564 PDVLQYMSEEKEVLALLSKLDLQVLSFAESLPEDANQSVHLVASEGLEAYLPLADMVDIS 623
            DVL Y+SEEKEVLALLSKLDLQ L FAE+LPEDANQSVHLVASEGLEAYLPLA MVDIS
Sbjct: 731 ADVLHYISEEKEVLALLSKLDLQSLRFAETLPEDANQSVHLVASEGLEAYLPLAGMVDIS 790

Query: 624 AELPRLHKRLLKMDGEYKGIKARLGSPKFLEKAPEEIVRAAQEKADEMEEKINLTQKRIA 669
           AE+ RL KRLLKM+GE+KG+KARLGSPKFLEKAPEEIVR AQEKA+EMEEKINLT+ RIA
Sbjct: 791 AEMQRLQKRLLKMEGEFKGLKARLGSPKFLEKAPEEIVRTAQEKAEEMEEKINLTRNRIA 845

		  

BLAST of Spo13623.1 vs. NCBI nr
Match: gi|731319204|ref|XP_010670135.1| (PREDICTED: valine--tRNA ligase, mitochondrial isoform X1 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 851.7 bits (2199), Expect = 9.400e-244
Identity = 459/608 (75.49%), Postives = 496/608 (81.58%), Query Frame = 1

		  

Query: 84  GLDRFEAREKLWKDLTETGLAVKKQPHTLRVPRSQRGGEVRIIEPLVSKQWFVTMEALAE 143
           GLDRFEAREKLW DL ETGLAVKK+PHTLRVPRSQRGGE  IIEPLVSKQWFVTME LAE
Sbjct: 371 GLDRFEAREKLWNDLLETGLAVKKEPHTLRVPRSQRGGE--IIEPLVSKQWFVTMEPLAE 430

Query: 144 KALHAVEKGDLTIIPDRFEKIYNHWLTNIKDWCISRQLWWGHRIPVWYIVGRDCEEEYIV 203
           KALHAVE GDLTI+PDRFEKIYNHWL+NIKDWCISRQLWWGHRIPVWYIVG+DCEEEYIV
Sbjct: 431 KALHAVETGDLTILPDRFEKIYNHWLSNIKDWCISRQLWWGHRIPVWYIVGKDCEEEYIV 490

Query: 204 ARSQEEALHKAQQKYGKDAEIYQDPDVLDTWFSSALWPFSTLGWPDVSQEEYKMFYPTTM 263
           ARS EEALHKAQQKYGKDAEIYQDPDVLDTWFSS+LWPFSTLGWPDVS +++K FYPTTM
Sbjct: 491 ARSSEEALHKAQQKYGKDAEIYQDPDVLDTWFSSSLWPFSTLGWPDVSDDDFKSFYPTTM 550

Query: 264 LETGHDILFFWVARMVMMGIEFTGNVPFRYVYLHGLIRDSQGRKMSKTLGNVIDPVDTIK 323
           LETGHDILFFWVARMVMMGIEFTGNVPFRYVYLHGLIRDSQGRKMSKTLGNVIDPVDTIK
Sbjct: 551 LETGHDILFFWVARMVMMGIEFTGNVPFRYVYLHGLIRDSQGRKMSKTLGNVIDPVDTIK 610

Query: 324 DFGTDALRFTLALGTAGQFDKEESVLSLRLPECWVVSKLHLLIDLVTTSYDKFYFGDVAR 383
           +FGTDALRFTLALGTAGQ D   S   L   + +  +KL         +  KF   ++  
Sbjct: 611 EFGTDALRFTLALGTAGQ-DLNLSAERLSANKAF-TNKL--------WNAGKFMLQNLPS 670

Query: 384 ETYDFFWGDFADW-YAYSNSLCAQILLNLKLQQQLYLLSILMKVS------------LFD 443
           ++  F W     + +   +SL    L    +  +L+ L  L+  S             +D
Sbjct: 671 QSDVFAWETLRAYEFDKEDSLLKLRLPECWVVSKLHGLIDLVTASYDKFYFGDVARETYD 730

Query: 444 FFDMNACLIRYIEASKARLYQSEDNSVTTAAQAVLLYVFENILKILHPFMPFVTEALWQA 503
           FF        YIEASKARLYQS D SVT+AAQAVLLYVFENILKILHPFMP+VTEALWQA
Sbjct: 731 FF-WGDFADWYIEASKARLYQSGDKSVTSAAQAVLLYVFENILKILHPFMPYVTEALWQA 790

Query: 504 IPNQKEPLIVSSWPKTSLPSYSNA----------TRAIRNVRAEYSVEPVKRISATIVAN 563
           IP QK PLIVSSWP TSLP YSNA          TRAIRN RAEYSVEPVKRI+AT+VAN
Sbjct: 791 IPYQKAPLIVSSWPTTSLPVYSNAIKRFENFQALTRAIRNARAEYSVEPVKRIAATVVAN 850

Query: 564 PDVLQYMSEEKEVLALLSKLDLQVLSFAESLPEDANQSVHLVASEGLEAYLPLADMVDIS 623
            DVL Y+SEEKEVLALLSKLDLQ L FAE+LPEDANQSVHLVASEGLEAYLPLA MVDIS
Sbjct: 851 ADVLHYISEEKEVLALLSKLDLQSLRFAETLPEDANQSVHLVASEGLEAYLPLAGMVDIS 910

Query: 624 AELPRLHKRLLKMDGEYKGIKARLGSPKFLEKAPEEIVRAAQEKADEMEEKINLTQKRIA 669
           AE+ RL KRLLKM+GE+KG+KARLGSPKFLEKAPEEIVR AQEKA+EMEEKINLT+ RIA
Sbjct: 911 AEMQRLQKRLLKMEGEFKGLKARLGSPKFLEKAPEEIVRTAQEKAEEMEEKINLTRNRIA 965

		  

BLAST of Spo13623.1 vs. NCBI nr
Match: gi|731319210|ref|XP_010670138.1| (PREDICTED: valine--tRNA ligase, mitochondrial isoform X4 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 851.7 bits (2199), Expect = 9.400e-244
Identity = 459/608 (75.49%), Postives = 496/608 (81.58%), Query Frame = 1

		  

Query: 84  GLDRFEAREKLWKDLTETGLAVKKQPHTLRVPRSQRGGEVRIIEPLVSKQWFVTMEALAE 143
           GLDRFEAREKLW DL ETGLAVKK+PHTLRVPRSQRGGE  IIEPLVSKQWFVTME LAE
Sbjct: 220 GLDRFEAREKLWNDLLETGLAVKKEPHTLRVPRSQRGGE--IIEPLVSKQWFVTMEPLAE 279

Query: 144 KALHAVEKGDLTIIPDRFEKIYNHWLTNIKDWCISRQLWWGHRIPVWYIVGRDCEEEYIV 203
           KALHAVE GDLTI+PDRFEKIYNHWL+NIKDWCISRQLWWGHRIPVWYIVG+DCEEEYIV
Sbjct: 280 KALHAVETGDLTILPDRFEKIYNHWLSNIKDWCISRQLWWGHRIPVWYIVGKDCEEEYIV 339

Query: 204 ARSQEEALHKAQQKYGKDAEIYQDPDVLDTWFSSALWPFSTLGWPDVSQEEYKMFYPTTM 263
           ARS EEALHKAQQKYGKDAEIYQDPDVLDTWFSS+LWPFSTLGWPDVS +++K FYPTTM
Sbjct: 340 ARSSEEALHKAQQKYGKDAEIYQDPDVLDTWFSSSLWPFSTLGWPDVSDDDFKSFYPTTM 399

Query: 264 LETGHDILFFWVARMVMMGIEFTGNVPFRYVYLHGLIRDSQGRKMSKTLGNVIDPVDTIK 323
           LETGHDILFFWVARMVMMGIEFTGNVPFRYVYLHGLIRDSQGRKMSKTLGNVIDPVDTIK
Sbjct: 400 LETGHDILFFWVARMVMMGIEFTGNVPFRYVYLHGLIRDSQGRKMSKTLGNVIDPVDTIK 459

Query: 324 DFGTDALRFTLALGTAGQFDKEESVLSLRLPECWVVSKLHLLIDLVTTSYDKFYFGDVAR 383
           +FGTDALRFTLALGTAGQ D   S   L   + +  +KL         +  KF   ++  
Sbjct: 460 EFGTDALRFTLALGTAGQ-DLNLSAERLSANKAF-TNKL--------WNAGKFMLQNLPS 519

Query: 384 ETYDFFWGDFADW-YAYSNSLCAQILLNLKLQQQLYLLSILMKVS------------LFD 443
           ++  F W     + +   +SL    L    +  +L+ L  L+  S             +D
Sbjct: 520 QSDVFAWETLRAYEFDKEDSLLKLRLPECWVVSKLHGLIDLVTASYDKFYFGDVARETYD 579

Query: 444 FFDMNACLIRYIEASKARLYQSEDNSVTTAAQAVLLYVFENILKILHPFMPFVTEALWQA 503
           FF        YIEASKARLYQS D SVT+AAQAVLLYVFENILKILHPFMP+VTEALWQA
Sbjct: 580 FF-WGDFADWYIEASKARLYQSGDKSVTSAAQAVLLYVFENILKILHPFMPYVTEALWQA 639

Query: 504 IPNQKEPLIVSSWPKTSLPSYSNA----------TRAIRNVRAEYSVEPVKRISATIVAN 563
           IP QK PLIVSSWP TSLP YSNA          TRAIRN RAEYSVEPVKRI+AT+VAN
Sbjct: 640 IPYQKAPLIVSSWPTTSLPVYSNAIKRFENFQALTRAIRNARAEYSVEPVKRIAATVVAN 699

Query: 564 PDVLQYMSEEKEVLALLSKLDLQVLSFAESLPEDANQSVHLVASEGLEAYLPLADMVDIS 623
            DVL Y+SEEKEVLALLSKLDLQ L FAE+LPEDANQSVHLVASEGLEAYLPLA MVDIS
Sbjct: 700 ADVLHYISEEKEVLALLSKLDLQSLRFAETLPEDANQSVHLVASEGLEAYLPLAGMVDIS 759

Query: 624 AELPRLHKRLLKMDGEYKGIKARLGSPKFLEKAPEEIVRAAQEKADEMEEKINLTQKRIA 669
           AE+ RL KRLLKM+GE+KG+KARLGSPKFLEKAPEEIVR AQEKA+EMEEKINLT+ RIA
Sbjct: 760 AEMQRLQKRLLKMEGEFKGLKARLGSPKFLEKAPEEIVRTAQEKAEEMEEKINLTRNRIA 814

		  

BLAST of Spo13623.1 vs. NCBI nr
Match: gi|590651897|ref|XP_007033012.1| (ATP binding,valine-tRNA ligase isoform 1 [Theobroma cacao])

HSP 1 Score: 767.3 bits (1980), Expect = 2.300e-218
Identity = 420/643 (65.32%), Postives = 468/643 (72.78%), Query Frame = 1

		  

Query: 84  GLDRFEAREKLWKDLTETGLAVKKQPHTLRVPRSQRGGEVRIIEPLVSKQWFVTMEALAE 143
           GLDRFEAR+KLW +L ET LAVKK+P+TLRVPRSQRGGEV  IEPLVSKQWFVTME LAE
Sbjct: 375 GLDRFEARKKLWCELEETDLAVKKEPYTLRVPRSQRGGEV--IEPLVSKQWFVTMEPLAE 434

Query: 144 KALHAVEKGDLTIIPDRFEKIYNHWLTNIKDWCISRQLWWGHRIPVWYIVGRDCEEEYIV 203
           KAL AVEKG+LTI+P+RFEKIYNHWL+NIKDWCISRQLWWGHRIPVWYIVG+DCEEEYIV
Sbjct: 435 KALRAVEKGELTIMPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWYIVGKDCEEEYIV 494

Query: 204 ARSQEEALHKAQQKYGKDAEIYQDPDVLDTWFSSALWPFSTLGWPDVSQEEYKMFYPTTM 263
           ARS EEAL KA  KYGK+ EIYQDPDVLDTWFSSALWPFSTLGWPDVS E++K FYPTTM
Sbjct: 495 ARSAEEALIKACDKYGKEIEIYQDPDVLDTWFSSALWPFSTLGWPDVSAEDFKRFYPTTM 554

Query: 264 LETGHDILFFWVARMVMMGIEFTGNVPFRYVYLHGLIRDSQGRKMSKTLGNVIDPVDTIK 323
           LETGHDILFFWVARMVMMGIEFTG VPF YVYLHGLIRDS+GRKMSKTLGNVIDP+DTIK
Sbjct: 555 LETGHDILFFWVARMVMMGIEFTGTVPFSYVYLHGLIRDSEGRKMSKTLGNVIDPLDTIK 614

Query: 324 DFGTDALR------------------------FTLALGTAGQF----------------- 383
           +FGTDALR                        FT  L  AG+F                 
Sbjct: 615 EFGTDALRFTLALGTAGQDLNLSTERLTANKAFTNKLWNAGKFVLQNLPDRDNVSGWQTI 674

Query: 384 -----DKEESVLSLRLPECWVVSKLHLLIDLVTTSYDKFYFGDVARETYD-FFWGDFA-D 443
                D EES+L L L ECWVVSKLHLL              D   E+Y+ FF+G+   +
Sbjct: 675 QAYKFDMEESLLRLPLSECWVVSKLHLL-------------IDAVTESYNKFFFGEVGRE 734

Query: 444 WYAYSNSLCAQILLNLKLQQQLYLLSILMKVSLFDFFDMNACLIRYIEASKARLYQSEDN 503
            Y +                              DF D       YIEASKARLY S D+
Sbjct: 735 TYDFIWG---------------------------DFADW------YIEASKARLYHSGDD 794

Query: 504 SVTTAAQAVLLYVFENILKILHPFMPFVTEALWQAIPNQKEPLIVSSWPKTSLPSYSNA- 563
           SV   AQAVLLYVFE+ILK+LHPFMPFVTE LWQA+PN+KE LI+SSWP+TSLP  +   
Sbjct: 795 SVALVAQAVLLYVFESILKLLHPFMPFVTEELWQALPNRKEALIISSWPQTSLPRNTTLV 854

Query: 564 ---------TRAIRNVRAEYSVEPVKRISATIVANPDVLQYMSEEKEVLALLSKLDLQVL 623
                    TRAIRN RAEYSVEP KRISA+IVA+ +V+QY+SEEKEVLALLS+LDL  +
Sbjct: 855 KRFENLQALTRAIRNARAEYSVEPAKRISASIVASEEVIQYISEEKEVLALLSRLDLDNI 914

Query: 624 SFAESLPEDANQSVHLVASEGLEAYLPLADMVDISAELPRLHKRLLKMDGEYKGIKARLG 669
            F +S P DA QSVHLVASEGLEAYLPLADMVDISAE+ RL KRL KM  EY+G+KARL 
Sbjct: 915 HFTDSPPGDAKQSVHLVASEGLEAYLPLADMVDISAEVQRLSKRLSKMQTEYEGLKARLK 969

		  

BLAST of Spo13623.1 vs. NCBI nr
Match: gi|590651908|ref|XP_007033015.1| (ATP binding,valine-tRNA ligase isoform 4 [Theobroma cacao])

HSP 1 Score: 767.3 bits (1980), Expect = 2.300e-218
Identity = 420/643 (65.32%), Postives = 468/643 (72.78%), Query Frame = 1

		  

Query: 84  GLDRFEAREKLWKDLTETGLAVKKQPHTLRVPRSQRGGEVRIIEPLVSKQWFVTMEALAE 143
           GLDRFEAR+KLW +L ET LAVKK+P+TLRVPRSQRGGEV  IEPLVSKQWFVTME LAE
Sbjct: 69  GLDRFEARKKLWCELEETDLAVKKEPYTLRVPRSQRGGEV--IEPLVSKQWFVTMEPLAE 128

Query: 144 KALHAVEKGDLTIIPDRFEKIYNHWLTNIKDWCISRQLWWGHRIPVWYIVGRDCEEEYIV 203
           KAL AVEKG+LTI+P+RFEKIYNHWL+NIKDWCISRQLWWGHRIPVWYIVG+DCEEEYIV
Sbjct: 129 KALRAVEKGELTIMPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWYIVGKDCEEEYIV 188

Query: 204 ARSQEEALHKAQQKYGKDAEIYQDPDVLDTWFSSALWPFSTLGWPDVSQEEYKMFYPTTM 263
           ARS EEAL KA  KYGK+ EIYQDPDVLDTWFSSALWPFSTLGWPDVS E++K FYPTTM
Sbjct: 189 ARSAEEALIKACDKYGKEIEIYQDPDVLDTWFSSALWPFSTLGWPDVSAEDFKRFYPTTM 248

Query: 264 LETGHDILFFWVARMVMMGIEFTGNVPFRYVYLHGLIRDSQGRKMSKTLGNVIDPVDTIK 323
           LETGHDILFFWVARMVMMGIEFTG VPF YVYLHGLIRDS+GRKMSKTLGNVIDP+DTIK
Sbjct: 249 LETGHDILFFWVARMVMMGIEFTGTVPFSYVYLHGLIRDSEGRKMSKTLGNVIDPLDTIK 308

Query: 324 DFGTDALR------------------------FTLALGTAGQF----------------- 383
           +FGTDALR                        FT  L  AG+F                 
Sbjct: 309 EFGTDALRFTLALGTAGQDLNLSTERLTANKAFTNKLWNAGKFVLQNLPDRDNVSGWQTI 368

Query: 384 -----DKEESVLSLRLPECWVVSKLHLLIDLVTTSYDKFYFGDVARETYD-FFWGDFA-D 443
                D EES+L L L ECWVVSKLHLL              D   E+Y+ FF+G+   +
Sbjct: 369 QAYKFDMEESLLRLPLSECWVVSKLHLL-------------IDAVTESYNKFFFGEVGRE 428

Query: 444 WYAYSNSLCAQILLNLKLQQQLYLLSILMKVSLFDFFDMNACLIRYIEASKARLYQSEDN 503
            Y +                              DF D       YIEASKARLY S D+
Sbjct: 429 TYDFIWG---------------------------DFADW------YIEASKARLYHSGDD 488

Query: 504 SVTTAAQAVLLYVFENILKILHPFMPFVTEALWQAIPNQKEPLIVSSWPKTSLPSYSNA- 563
           SV   AQAVLLYVFE+ILK+LHPFMPFVTE LWQA+PN+KE LI+SSWP+TSLP  +   
Sbjct: 489 SVALVAQAVLLYVFESILKLLHPFMPFVTEELWQALPNRKEALIISSWPQTSLPRNTTLV 548

Query: 564 ---------TRAIRNVRAEYSVEPVKRISATIVANPDVLQYMSEEKEVLALLSKLDLQVL 623
                    TRAIRN RAEYSVEP KRISA+IVA+ +V+QY+SEEKEVLALLS+LDL  +
Sbjct: 549 KRFENLQALTRAIRNARAEYSVEPAKRISASIVASEEVIQYISEEKEVLALLSRLDLDNI 608

Query: 624 SFAESLPEDANQSVHLVASEGLEAYLPLADMVDISAELPRLHKRLLKMDGEYKGIKARLG 669
            F +S P DA QSVHLVASEGLEAYLPLADMVDISAE+ RL KRL KM  EY+G+KARL 
Sbjct: 609 HFTDSPPGDAKQSVHLVASEGLEAYLPLADMVDISAEVQRLSKRLSKMQTEYEGLKARLK 663

		  

BLAST of Spo13623.1 vs. UniProtKB/TrEMBL
Match: A0A061EGC7_THECC (ATP binding,valine-tRNA ligase isoform 4 OS=Theobroma cacao GN=TCM_019154 PE=4 SV=1)

HSP 1 Score: 767.3 bits (1980), Expect = 1.600e-218
Identity = 420/643 (65.32%), Postives = 468/643 (72.78%), Query Frame = 1

		  

Query: 84  GLDRFEAREKLWKDLTETGLAVKKQPHTLRVPRSQRGGEVRIIEPLVSKQWFVTMEALAE 143
           GLDRFEAR+KLW +L ET LAVKK+P+TLRVPRSQRGGEV  IEPLVSKQWFVTME LAE
Sbjct: 69  GLDRFEARKKLWCELEETDLAVKKEPYTLRVPRSQRGGEV--IEPLVSKQWFVTMEPLAE 128

Query: 144 KALHAVEKGDLTIIPDRFEKIYNHWLTNIKDWCISRQLWWGHRIPVWYIVGRDCEEEYIV 203
           KAL AVEKG+LTI+P+RFEKIYNHWL+NIKDWCISRQLWWGHRIPVWYIVG+DCEEEYIV
Sbjct: 129 KALRAVEKGELTIMPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWYIVGKDCEEEYIV 188

Query: 204 ARSQEEALHKAQQKYGKDAEIYQDPDVLDTWFSSALWPFSTLGWPDVSQEEYKMFYPTTM 263
           ARS EEAL KA  KYGK+ EIYQDPDVLDTWFSSALWPFSTLGWPDVS E++K FYPTTM
Sbjct: 189 ARSAEEALIKACDKYGKEIEIYQDPDVLDTWFSSALWPFSTLGWPDVSAEDFKRFYPTTM 248

Query: 264 LETGHDILFFWVARMVMMGIEFTGNVPFRYVYLHGLIRDSQGRKMSKTLGNVIDPVDTIK 323
           LETGHDILFFWVARMVMMGIEFTG VPF YVYLHGLIRDS+GRKMSKTLGNVIDP+DTIK
Sbjct: 249 LETGHDILFFWVARMVMMGIEFTGTVPFSYVYLHGLIRDSEGRKMSKTLGNVIDPLDTIK 308

Query: 324 DFGTDALR------------------------FTLALGTAGQF----------------- 383
           +FGTDALR                        FT  L  AG+F                 
Sbjct: 309 EFGTDALRFTLALGTAGQDLNLSTERLTANKAFTNKLWNAGKFVLQNLPDRDNVSGWQTI 368

Query: 384 -----DKEESVLSLRLPECWVVSKLHLLIDLVTTSYDKFYFGDVARETYD-FFWGDFA-D 443
                D EES+L L L ECWVVSKLHLL              D   E+Y+ FF+G+   +
Sbjct: 369 QAYKFDMEESLLRLPLSECWVVSKLHLL-------------IDAVTESYNKFFFGEVGRE 428

Query: 444 WYAYSNSLCAQILLNLKLQQQLYLLSILMKVSLFDFFDMNACLIRYIEASKARLYQSEDN 503
            Y +                              DF D       YIEASKARLY S D+
Sbjct: 429 TYDFIWG---------------------------DFADW------YIEASKARLYHSGDD 488

Query: 504 SVTTAAQAVLLYVFENILKILHPFMPFVTEALWQAIPNQKEPLIVSSWPKTSLPSYSNA- 563
           SV   AQAVLLYVFE+ILK+LHPFMPFVTE LWQA+PN+KE LI+SSWP+TSLP  +   
Sbjct: 489 SVALVAQAVLLYVFESILKLLHPFMPFVTEELWQALPNRKEALIISSWPQTSLPRNTTLV 548

Query: 564 ---------TRAIRNVRAEYSVEPVKRISATIVANPDVLQYMSEEKEVLALLSKLDLQVL 623
                    TRAIRN RAEYSVEP KRISA+IVA+ +V+QY+SEEKEVLALLS+LDL  +
Sbjct: 549 KRFENLQALTRAIRNARAEYSVEPAKRISASIVASEEVIQYISEEKEVLALLSRLDLDNI 608

Query: 624 SFAESLPEDANQSVHLVASEGLEAYLPLADMVDISAELPRLHKRLLKMDGEYKGIKARLG 669
            F +S P DA QSVHLVASEGLEAYLPLADMVDISAE+ RL KRL KM  EY+G+KARL 
Sbjct: 609 HFTDSPPGDAKQSVHLVASEGLEAYLPLADMVDISAEVQRLSKRLSKMQTEYEGLKARLK 663

		  

BLAST of Spo13623.1 vs. UniProtKB/TrEMBL
Match: A0A061EGZ7_THECC (ATP binding,valine-tRNA ligase isoform 1 OS=Theobroma cacao GN=TCM_019154 PE=3 SV=1)

HSP 1 Score: 767.3 bits (1980), Expect = 1.600e-218
Identity = 420/643 (65.32%), Postives = 468/643 (72.78%), Query Frame = 1

		  

Query: 84  GLDRFEAREKLWKDLTETGLAVKKQPHTLRVPRSQRGGEVRIIEPLVSKQWFVTMEALAE 143
           GLDRFEAR+KLW +L ET LAVKK+P+TLRVPRSQRGGEV  IEPLVSKQWFVTME LAE
Sbjct: 375 GLDRFEARKKLWCELEETDLAVKKEPYTLRVPRSQRGGEV--IEPLVSKQWFVTMEPLAE 434

Query: 144 KALHAVEKGDLTIIPDRFEKIYNHWLTNIKDWCISRQLWWGHRIPVWYIVGRDCEEEYIV 203
           KAL AVEKG+LTI+P+RFEKIYNHWL+NIKDWCISRQLWWGHRIPVWYIVG+DCEEEYIV
Sbjct: 435 KALRAVEKGELTIMPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWYIVGKDCEEEYIV 494

Query: 204 ARSQEEALHKAQQKYGKDAEIYQDPDVLDTWFSSALWPFSTLGWPDVSQEEYKMFYPTTM 263
           ARS EEAL KA  KYGK+ EIYQDPDVLDTWFSSALWPFSTLGWPDVS E++K FYPTTM
Sbjct: 495 ARSAEEALIKACDKYGKEIEIYQDPDVLDTWFSSALWPFSTLGWPDVSAEDFKRFYPTTM 554

Query: 264 LETGHDILFFWVARMVMMGIEFTGNVPFRYVYLHGLIRDSQGRKMSKTLGNVIDPVDTIK 323
           LETGHDILFFWVARMVMMGIEFTG VPF YVYLHGLIRDS+GRKMSKTLGNVIDP+DTIK
Sbjct: 555 LETGHDILFFWVARMVMMGIEFTGTVPFSYVYLHGLIRDSEGRKMSKTLGNVIDPLDTIK 614

Query: 324 DFGTDALR------------------------FTLALGTAGQF----------------- 383
           +FGTDALR                        FT  L  AG+F                 
Sbjct: 615 EFGTDALRFTLALGTAGQDLNLSTERLTANKAFTNKLWNAGKFVLQNLPDRDNVSGWQTI 674

Query: 384 -----DKEESVLSLRLPECWVVSKLHLLIDLVTTSYDKFYFGDVARETYD-FFWGDFA-D 443
                D EES+L L L ECWVVSKLHLL              D   E+Y+ FF+G+   +
Sbjct: 675 QAYKFDMEESLLRLPLSECWVVSKLHLL-------------IDAVTESYNKFFFGEVGRE 734

Query: 444 WYAYSNSLCAQILLNLKLQQQLYLLSILMKVSLFDFFDMNACLIRYIEASKARLYQSEDN 503
            Y +                              DF D       YIEASKARLY S D+
Sbjct: 735 TYDFIWG---------------------------DFADW------YIEASKARLYHSGDD 794

Query: 504 SVTTAAQAVLLYVFENILKILHPFMPFVTEALWQAIPNQKEPLIVSSWPKTSLPSYSNA- 563
           SV   AQAVLLYVFE+ILK+LHPFMPFVTE LWQA+PN+KE LI+SSWP+TSLP  +   
Sbjct: 795 SVALVAQAVLLYVFESILKLLHPFMPFVTEELWQALPNRKEALIISSWPQTSLPRNTTLV 854

Query: 564 ---------TRAIRNVRAEYSVEPVKRISATIVANPDVLQYMSEEKEVLALLSKLDLQVL 623
                    TRAIRN RAEYSVEP KRISA+IVA+ +V+QY+SEEKEVLALLS+LDL  +
Sbjct: 855 KRFENLQALTRAIRNARAEYSVEPAKRISASIVASEEVIQYISEEKEVLALLSRLDLDNI 914

Query: 624 SFAESLPEDANQSVHLVASEGLEAYLPLADMVDISAELPRLHKRLLKMDGEYKGIKARLG 669
            F +S P DA QSVHLVASEGLEAYLPLADMVDISAE+ RL KRL KM  EY+G+KARL 
Sbjct: 915 HFTDSPPGDAKQSVHLVASEGLEAYLPLADMVDISAEVQRLSKRLSKMQTEYEGLKARLK 969

		  

BLAST of Spo13623.1 vs. UniProtKB/TrEMBL
Match: A0A067KDU4_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_13438 PE=3 SV=1)

HSP 1 Score: 761.5 bits (1965), Expect = 8.900e-217
Identity = 409/643 (63.61%), Postives = 465/643 (72.32%), Query Frame = 1

		  

Query: 84  GLDRFEAREKLWKDLTETGLAVKKQPHTLRVPRSQRGGEVRIIEPLVSKQWFVTMEALAE 143
           GLDRFEAR+KLW DL ETGLAVKK+PHTLRVPRSQRGGE  IIEPLVSKQWFVTME LAE
Sbjct: 369 GLDRFEARKKLWSDLEETGLAVKKEPHTLRVPRSQRGGE--IIEPLVSKQWFVTMEPLAE 428

Query: 144 KALHAVEKGDLTIIPDRFEKIYNHWLTNIKDWCISRQLWWGHRIPVWYIVGRDCEEEYIV 203
           KAL AVEKG++TIIP+RFEKIYNHWL+NIKDWCISRQLWWGHRIPVWYIVG++CEE+YIV
Sbjct: 429 KALRAVEKGEITIIPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWYIVGKNCEEDYIV 488

Query: 204 ARSQEEALHKAQQKYGKDAEIYQDPDVLDTWFSSALWPFSTLGWPDVSQEEYKMFYPTTM 263
           AR+ ++AL KA +KYGKD EIYQDPDVLDTWFSSALWPFSTLGWPD+S ++++ FYPTTM
Sbjct: 489 ARNADDALQKAHEKYGKDVEIYQDPDVLDTWFSSALWPFSTLGWPDISSDDFEKFYPTTM 548

Query: 264 LETGHDILFFWVARMVMMGIEFTGNVPFRYVYLHGLIRDSQGRKMSKTLGNVIDPVDTIK 323
           LETGHDILFFWVARM+MMGIEFTG VPF YVYLHGLIRDSQGRKMSKTLGNVIDP+DTIK
Sbjct: 549 LETGHDILFFWVARMIMMGIEFTGKVPFSYVYLHGLIRDSQGRKMSKTLGNVIDPLDTIK 608

Query: 324 DFGTD------------------------ALRFTLALGTAGQF----------------- 383
           DFGTD                           FT  L  AG+F                 
Sbjct: 609 DFGTDALRFTLALGTAGQDLNLSTERLTANKAFTNKLWNAGKFVLQNLPSTTDISAWENV 668

Query: 384 -----DKEESVLSLRLPECWVVSKLHLLIDLVTTSYDKFYFGDVARETYDFFW-GDFA-D 443
                D E+S+L L LPECWVVSKLH+L              D A  +YD F+ GD   +
Sbjct: 669 MAYKFDNEDSLLKLPLPECWVVSKLHIL-------------IDTATASYDKFFFGDVGRE 728

Query: 444 WYAYSNSLCAQILLNLKLQQQLYLLSILMKVSLFDFFDMNACLIRYIEASKARLYQSEDN 503
            Y +  S                           DF D       YIEASKARLYQS  +
Sbjct: 729 TYDFFWS---------------------------DFADW------YIEASKARLYQSGGD 788

Query: 504 SVTTAAQAVLLYVFENILKILHPFMPFVTEALWQAIPNQKEPLIVSSWPKTSLP------ 563
           S  + AQAVLLYVFEN+LK+LHPFMPFVTE LWQA+P + E LIVS WP+ SLP      
Sbjct: 789 SAASVAQAVLLYVFENVLKLLHPFMPFVTEELWQALPQRNEALIVSPWPQISLPRNASSI 848

Query: 564 ----SYSNATRAIRNVRAEYSVEPVKRISATIVANPDVLQYMSEEKEVLALLSKLDLQVL 623
               ++   TRAIRN RAEYSVEP KRISA+IVA+ +V+QY+S+EKEVLALLS+LDLQ +
Sbjct: 849 KKFENFQALTRAIRNARAEYSVEPAKRISASIVASEEVIQYISKEKEVLALLSRLDLQNV 908

Query: 624 SFAESLPEDANQSVHLVASEGLEAYLPLADMVDISAELPRLHKRLLKMDGEYKGIKARLG 669
            F +SLP DANQSVHLVASEGLEAYLPLADMVDISAE+ RL KRL KM  EY+ + ARL 
Sbjct: 909 HFTDSLPRDANQSVHLVASEGLEAYLPLADMVDISAEVDRLSKRLSKMQTEYEALAARLN 963

		  

BLAST of Spo13623.1 vs. UniProtKB/TrEMBL
Match: U5GC79_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0005s14610g PE=3 SV=1)

HSP 1 Score: 760.8 bits (1963), Expect = 1.500e-216
Identity = 408/614 (66.45%), Postives = 478/614 (77.85%), Query Frame = 1

		  

Query: 84  GLDRFEAREKLWKDLTETGLAVKKQPHTLRVPRSQRGGEVRIIEPLVSKQWFVTMEALAE 143
           GLDRFEAR+KLW +L ETGLA+KK+PHTLRVPRSQRGGE  IIEPLVSKQWFVTME LAE
Sbjct: 376 GLDRFEARKKLWSELEETGLAIKKEPHTLRVPRSQRGGE--IIEPLVSKQWFVTMEPLAE 435

Query: 144 KALHAVEKGDLTIIPDRFEKIYNHWLTNIKDWCISRQLWWGHRIPVWYIVGRDCEEEYIV 203
           KAL AVEKG+LTI+P+RFEKIYNHWL+NIKDWCISRQLWWGHRIPVWYIVG++CEE+YIV
Sbjct: 436 KALRAVEKGELTIMPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWYIVGKNCEEDYIV 495

Query: 204 ARSQEEALHKAQQKYGKDAEIYQDPDVLDTWFSSALWPFSTLGWPDVSQEEYKMFYPTTM 263
           AR+ +EAL KA++KYGK+ EIYQDPDVLDTWFSSALWPFSTLGWPDVS E++K FYPTTM
Sbjct: 496 ARNADEALEKAREKYGKNVEIYQDPDVLDTWFSSALWPFSTLGWPDVSAEDFKKFYPTTM 555

Query: 264 LETGHDILFFWVARMVMMGIEFTGNVPFRYVYLHGLIRDSQGRKMSKTLGNVIDPVDTIK 323
           LETGHDILFFWVARMVMMGIEFTG VPF YVYLHGLIRDSQGRKMSKTLGNVIDP+DTIK
Sbjct: 556 LETGHDILFFWVARMVMMGIEFTGTVPFSYVYLHGLIRDSQGRKMSKTLGNVIDPLDTIK 615

Query: 324 DFGTDALRFTLALGTAGQFDKEESVLSLRLPECWVVSKLHLLIDLVTTSYDKFYFGDVAR 383
           +FGTDALRFT++LGTAGQ D   S   L   + +  +KL         +  KF   ++  
Sbjct: 616 EFGTDALRFTISLGTAGQ-DLNLSTERLTANKAF-TNKL--------WNAGKFVLQNMPS 675

Query: 384 ETYDFFWGDFADWYAYSNSLC--AQILLNLKLQQ-----QLYLLSILMKVS--------- 443
           +T      D + W A  N      + +L L L +     +L++L  ++  S         
Sbjct: 676 QT------DVSAWEAIRNCKFDKEESVLRLPLPECWVVSELHVLIDMVTASYDKFFFGDV 735

Query: 444 ---LFDFFDMNACLIRYIEASKARLYQSEDNSVTTAAQAVLLYVFENILKILHPFMPFVT 503
              ++DFF  +     YIEASKARLYQS  NS  + AQAVLLYVF+N+LK+LHPFMPFVT
Sbjct: 736 GREIYDFF-WSDFADWYIEASKARLYQSGANSACSEAQAVLLYVFKNVLKLLHPFMPFVT 795

Query: 504 EALWQAIPNQKEPLIVSSWPKTSLPSYSNA----------TRAIRNVRAEYSVEPVKRIS 563
           E LWQA+P+ KE LIVS WP+TSLP + N+          TRAIRN RAEYSVEP KRIS
Sbjct: 796 EELWQALPDPKEALIVSPWPQTSLPRFPNSIKKFENFQALTRAIRNARAEYSVEPAKRIS 855

Query: 564 ATIVANPDVLQYMSEEKEVLALLSKLDLQVLSFAESLPEDANQSVHLVASEGLEAYLPLA 623
           A+IVA+ +V+QY+S EKEVLALLS+LDLQ + F +S P DANQSVHLVASEGLEAYLPLA
Sbjct: 856 ASIVASEEVIQYISNEKEVLALLSRLDLQNIHFTDSPPGDANQSVHLVASEGLEAYLPLA 915

Query: 624 DMVDISAELPRLHKRLLKMDGEYKGIKARLGSPKFLEKAPEEIVRAAQEKADEMEEKINL 669
           DMV+ISAE+ RL KRL KM  EY G+ ARL S KF+EKAPE++VR  +EKA E EEKI L
Sbjct: 916 DMVNISAEVERLSKRLSKMQVEYDGLAARLSSRKFVEKAPEDVVRGVREKAAEAEEKIKL 970

		  

BLAST of Spo13623.1 vs. UniProtKB/TrEMBL
Match: A0A022RVQ9_ERYGU (Uncharacterized protein OS=Erythranthe guttata GN=MIMGU_mgv1a001930mg PE=4 SV=1)

HSP 1 Score: 757.7 bits (1955), Expect = 1.300e-215
Identity = 405/644 (62.89%), Postives = 469/644 (72.83%), Query Frame = 1

		  

Query: 84  GLDRFEAREKLWKDLTETGLAVKKQPHTLRVPRSQRGGEVRIIEPLVSKQWFVTMEALAE 143
           GLDRFEAR+KLW +L ETGLAVKK+ HTLRVPRSQRGGE  IIEPLVSKQWFVTME LAE
Sbjct: 142 GLDRFEARKKLWSELEETGLAVKKEVHTLRVPRSQRGGE--IIEPLVSKQWFVTMEPLAE 201

Query: 144 KALHAVEKGDLTIIPDRFEKIYNHWLTNIKDWCISRQLWWGHRIPVWYIVGRDCEEEYIV 203
           KAL AVEKG+LTI+P+RFEKIYNHWL+NIKDWCISRQLWWGHRIPVWYIVG+DCEE+YIV
Sbjct: 202 KALQAVEKGELTIMPERFEKIYNHWLSNIKDWCISRQLWWGHRIPVWYIVGKDCEEDYIV 261

Query: 204 ARSQEEALHKAQQKYGKDAEIYQDPDVLDTWFSSALWPFSTLGWPDVSQEEYKMFYPTTM 263
           A   +EAL KA++KYG++ EIYQDPDVLDTWFSSALWPFSTLGWPDVS +++K FYPT++
Sbjct: 262 ASCTDEALEKARKKYGQNVEIYQDPDVLDTWFSSALWPFSTLGWPDVSAKDFKEFYPTSV 321

Query: 264 LETGHDILFFWVARMVMMGIEFTGNVPFRYVYLHGLIRDSQGRKMSKTLGNVIDPVDTIK 323
           LETGHDILFFWVARMVMMGIEFTG VPF  +YLHGLIRDSQGRKMSKTLGNVIDP+DTIK
Sbjct: 322 LETGHDILFFWVARMVMMGIEFTGKVPFSNIYLHGLIRDSQGRKMSKTLGNVIDPLDTIK 381

Query: 324 DFGTDALR------------------------FTLALGTAGQF----------------- 383
           ++GTDALR                        FT  L  AG+F                 
Sbjct: 382 EYGTDALRFSLSLGTPGQDLNLSTERLNSNKAFTNKLWNAGKFVLQNLPPQSDLSACKAM 441

Query: 384 -----DKEESVLSLRLPECWVVSKLHLLIDLVTTSYDKFYFGDVARETYD-FFWGDFADW 443
                D EES+  L LPECWVVSKLH+L              D    +YD FF+GD A  
Sbjct: 442 QEFKFDTEESLFKLPLPECWVVSKLHML-------------IDAVTTSYDKFFFGDVAR- 501

Query: 444 YAYSNSLCAQILLNLKLQQQLYLLSILMKVSLFDFFDMNACLIRYIEASKARLYQSEDNS 503
                                          ++DFF  +     YIEASK+RLYQS  +S
Sbjct: 502 ------------------------------EIYDFFWADFA-DWYIEASKSRLYQSGGDS 561

Query: 504 VTTAAQAVLLYVFENILKILHPFMPFVTEALWQAIPNQKEPLIVSSWPKTSLPSYSNA-- 563
             + AQAVLLYVFENILK+LHPFMPFVTE LWQ++PN++E LIVS+WP TSLP  +++  
Sbjct: 562 TASVAQAVLLYVFENILKLLHPFMPFVTEELWQSLPNREEALIVSAWPVTSLPRQTDSIK 621

Query: 564 --------TRAIRNVRAEYSVEPVKRISATIVANPDVLQYMSEEKEVLALLSKLDLQVLS 623
                   TRAIRN RAEYSVEP +RISA+IVAN +V+QY+S+E+EVLALLS+LDLQ +S
Sbjct: 622 KFENLQALTRAIRNARAEYSVEPARRISASIVANSEVIQYISKEREVLALLSRLDLQNVS 681

Query: 624 FAESLPEDANQSVHLVASEGLEAYLPLADMVDISAELPRLHKRLLKMDGEYKGIKARLGS 671
           F +S P DANQSVHLVASEGLEAYLPLADMVDIS+E+ RL KRL KM  EY G+ ARL S
Sbjct: 682 FTQSPPGDANQSVHLVASEGLEAYLPLADMVDISSEVERLSKRLAKMQTEYDGLMARLSS 738

		  

BLAST of Spo13623.1 vs. ExPASy Swiss-Prot
Match: SYVM2_ARATH (Valine--tRNA ligase, chloroplastic/mitochondrial 2 OS=Arabidopsis thaliana GN=EMB2247 PE=3 SV=1)

HSP 1 Score: 718.8 bits (1854), Expect = 6.000e-206
Identity = 389/601 (64.73%), Postives = 453/601 (75.37%), Query Frame = 1

		  

Query: 84  GLDRFEAREKLWKDLTETGLAVKKQPHTLRVPRSQRGGEVRIIEPLVSKQWFVTMEALAE 143
           GLDRFE REKLW DL E GLAVKK+PHTLRVPRSQRGGEV  IEPLVSKQWFV M+ LAE
Sbjct: 377 GLDRFEVREKLWADLEEIGLAVKKEPHTLRVPRSQRGGEV--IEPLVSKQWFVHMDPLAE 436

Query: 144 KALHAVEKGDLTIIPDRFEKIYNHWLTNIKDWCISRQLWWGHRIPVWYIVGRDCEEEYIV 203
           KAL AVE  +LTIIP+RFEKIYNHWLTNIKDWCISRQLWWGHRIPVWY+VG+DCEE+YIV
Sbjct: 437 KALLAVENKELTIIPERFEKIYNHWLTNIKDWCISRQLWWGHRIPVWYVVGKDCEEDYIV 496

Query: 204 ARSQEEALHKAQQKYGKDAEIYQDPDVLDTWFSSALWPFSTLGWPDVSQEEYKMFYPTTM 263
           A+S EEAL KA +KYGKD EIYQDPDVLDTWFSS+LWPFSTLGWPDV+ +++  FYPT M
Sbjct: 497 AKSAEEALEKALEKYGKDVEIYQDPDVLDTWFSSSLWPFSTLGWPDVAAKDFNNFYPTNM 556

Query: 264 LETGHDILFFWVARMVMMGIEFTGNVPFRYVYLHGLIRDSQGRKMSKTLGNVIDPVDTIK 323
           LETGHDILFFWVARMVMMGIEFTG VPF +VYLHGLIRDSQGRKMSK+LGNVIDP+DTIK
Sbjct: 557 LETGHDILFFWVARMVMMGIEFTGTVPFSHVYLHGLIRDSQGRKMSKSLGNVIDPLDTIK 616

Query: 324 DFGTDALRFTLALGTAGQFDKEESVLSLRLPE-------CWVVSK--LHLLIDLVTTSYD 383
           DFGTDALRFT+ALGTAGQ   + ++ + RL          W   K  LH L  L  TS  
Sbjct: 617 DFGTDALRFTIALGTAGQ---DLNLSTERLTANKAFTNKLWNAGKFVLHSLPSLSDTSAW 676

Query: 384 KFYFGDVARETYDFFWGDFADWYAYSNSLCAQILLNLKLQQQLYLLSILMKVSLFDFFDM 443
           +        +          + +A S      IL++        L    +    +DFF  
Sbjct: 677 ENLLDLKLDKEETLLSLPLPECWAVSK---LHILIDSVTASYEKLFFGDVGRETYDFF-W 736

Query: 444 NACLIRYIEASKARLYQSEDNSVTTAAQAVLLYVFENILKILHPFMPFVTEALWQAIPNQ 503
           +     YIEASK+RLY S  NSV+ A+QAVLLYVFENILK+LHPFMPFVTE LWQA+P +
Sbjct: 737 SDFADWYIEASKSRLYGSGGNSVSLASQAVLLYVFENILKLLHPFMPFVTEDLWQALPYR 796

Query: 504 KEPLIVSSWPKTSLP----------SYSNATRAIRNVRAEYSVEPVKRISATIVANPDVL 563
           KE LIVS WP+ SLP          +    TRAIRN RAEYSVEPVKRISA++V + +V+
Sbjct: 797 KEALIVSPWPQNSLPRNVESIKRFENLQALTRAIRNARAEYSVEPVKRISASVVGSAEVI 856

Query: 564 QYMSEEKEVLALLSKLDLQVLSFAESLPEDANQSVHLVASEGLEAYLPLADMVDISAELP 623
           +Y+S+EKEVLALLS+LDL  + F+ + P DAN SVHLVASEGLEAYLPLA MVDIS+E+ 
Sbjct: 857 EYISKEKEVLALLSRLDLNNVHFSNAPPGDANLSVHLVASEGLEAYLPLAAMVDISSEVQ 916

Query: 624 RLHKRLLKMDGEYKGIKARLGSPKFLEKAPEEIVRAAQEKADEMEEKINLTQKRIAFLES 666
           R+ KRL KM  EY  +  RL SPKF+EKAPEE+VR  +E+ +E+EEKI LT+ R+ FL+S
Sbjct: 917 RISKRLSKMQTEYDALITRLSSPKFVEKAPEEVVRGVKEQVEELEEKIKLTKARLDFLKS 968

		  

BLAST of Spo13623.1 vs. ExPASy Swiss-Prot
Match: SYV_CALS4 (Valine--tRNA ligase OS=Caldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) GN=valS PE=3 SV=1)

HSP 1 Score: 387.9 bits (995), Expect = 2.400e-106
Identity = 239/602 (39.70%), Postives = 352/602 (58.47%), Query Frame = 1

		  

Query: 84  GLDRFEAREKLWKDLTETGLAVKKQPHTLRVPRSQRGGEVRIIEPLVSKQWFVTMEALAE 143
           GLDR+EAREK+ KDL E GL +K + H   V    R   V  +EPL+S+QWFV ME LA+
Sbjct: 313 GLDRYEAREKIVKDLEEQGLLLKVEEHVHNVGHCYRCDTV--VEPLLSEQWFVKMEPLAK 372

Query: 144 KALHAVEKGDLTIIPDRFEKIYNHWLTNIKDWCISRQLWWGHRIPVWYIVGRDCEEEYIV 203
            AL  V++G +  +P+RFEKIY +WL NIKDWCISRQLWWGHRIP WY     CE+   V
Sbjct: 373 PALQVVKEGKIKFVPERFEKIYTNWLENIKDWCISRQLWWGHRIPAWY-----CEDCGHV 432

Query: 204 ARSQEEALHKAQQKYGKDAEIYQDPDVLDTWFSSALWPFSTLGWPDVSQEEYKMFYPTTM 263
             S+E+ +   + +      I+QD +VLDTWFSSALWPFST+GWP+ + E+ K FYPT +
Sbjct: 433 TVSREDPV---KCEVCGSTNIHQDENVLDTWFSSALWPFSTMGWPEET-EDLKYFYPTDV 492

Query: 264 LETGHDILFFWVARMVMMGIEFTGNVPFRYVYLHGLIRDSQGRKMSKTLGNVIDPVDTIK 323
           L TG+DI+FFWVARM+ M +EF   +PF+YV +HGL+RD+ GRKMSK+LGN IDP++ I+
Sbjct: 493 LVTGYDIIFFWVARMIFMSLEFMKEIPFKYVLIHGLVRDALGRKMSKSLGNGIDPLEVIE 552

Query: 324 DFGTDALRFTLALGTAGQFDKE---ESVLSLR--LPECWVVSKLHLL-IDLVTTSYDKFY 383
            +G D LRFTL +G A   D     E V   R    + W  S+  LL +D   T+    Y
Sbjct: 553 KYGADTLRFTLVIGNAPGNDMRFSWEKVEHSRNFANKLWNASRYVLLNLDENDTN---LY 612

Query: 384 FGDVARETYDFFWGDFADWYAYS--NSLCAQILLNLKLQQQLYLLSILMKVSLFDFFDMN 443
             ++A           AD +  +  N++  +I  NL    + + L +     L+DF    
Sbjct: 613 LDNLA----------LADKWILTRYNNIVKEITDNL----EKFELGVAAS-KLYDFVWSE 672

Query: 444 ACLIRYIEASKARLYQSEDNSVTTAAQAVLLYVFENILKILHPFMPFVTEALWQAIPNQK 503
            C   YIE SK  LY S++       ++VL YV +N L++LHPFMPF+TE +WQ +P++ 
Sbjct: 673 FC-DWYIELSKPVLY-SDNEEAKKVTKSVLRYVLDNTLRLLHPFMPFITEEIWQNLPHEG 732

Query: 504 EPLIVSSWPK-----------TSLPSYSNATRAIRNVRAEYSVEPVKRISATIVANPDVL 563
           E ++V+ WPK                   A RAIRN+RAE +V P K+    I    +  
Sbjct: 733 ESIMVAEWPKYREDLNFEEDAKKAEIIMEAIRAIRNIRAEANVSPSKKAKVIIAVEKEEH 792

Query: 564 Q--YMSEEKEVLALLSKLDLQVLSFAESLPEDANQSVHLVASEGLEAYLPLADMVDISAE 623
           +  + S +  ++ L    ++ +    +++P+ A  +     S GL A +PL +++D+  E
Sbjct: 793 EKIFESGKNYIMKLAGASEVAIERNRDNIPQKAMSAA---ISAGLIA-VPLEELIDLEEE 852

Query: 624 LPRLHKRLLKMDGEYKGIKARLGSPKFLEKAPEEIVRAAQEKADEMEEKINLTQKRIAFL 665
           + RL +   K+  E +  ++ L +  F++KAPE++V A +EK ++    +   ++R++ L
Sbjct: 853 IKRLEEEREKVLKEIERAQSLLNNENFVKKAPEKVVNAEREKLEKYTAMLKNIEERLSLL 879

		  

BLAST of Spo13623.1 vs. ExPASy Swiss-Prot
Match: SYV_CLOAB (Valine--tRNA ligase OS=Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) GN=valS PE=3 SV=1)

HSP 1 Score: 378.3 bits (970), Expect = 1.900e-103
Identity = 240/601 (39.93%), Postives = 342/601 (56.91%), Query Frame = 1

		  

Query: 84  GLDRFEAREKLWKDLTETGLAVKKQPHTLRVPRSQRGGEVRIIEPLVSKQWFVTMEALAE 143
           G+DR+EAR+ +  DL E G  VK + H   V    R G   IIEP+VSKQW+V ME+LA+
Sbjct: 315 GMDRYEARKAIVSDLKEEGYLVKIKEHVHNVGTHDRCGN--IIEPMVSKQWYVKMESLAK 374

Query: 144 KALHAVEKGDLTIIPDRFEKIYNHWLTNIKDWCISRQLWWGHRIPVWYIVGRDCEEEYIV 203
            A+ AV+ G    +P+RF+KIY +W+ NI+DWCISRQLWWGHRIPVWY   +DC E  IV
Sbjct: 375 PAIEAVKAGKTKFVPERFDKIYFNWMENIQDWCISRQLWWGHRIPVWYC--KDCGE-IIV 434

Query: 204 ARSQEEALHKAQQKYGKDAEIYQDPDVLDTWFSSALWPFSTLGWPDVSQEEYKMFYPTTM 263
           +  + +A  K   +      + QD DVLDTWFSSALWPFSTLGWPD   E+ + FYPT  
Sbjct: 435 SEKEPKACSKCNSE-----NLEQDKDVLDTWFSSALWPFSTLGWPD-KNEDLEYFYPTDT 494

Query: 264 LETGHDILFFWVARMVMMGIEFTGNVPFRYVYLHGLIRDSQGRKMSKTLGNVIDPVDTIK 323
           L TG+DI+FFWVARMV  GI   G VPF++VY+HGL+RD++GRKMSK+LGN +DP+D I 
Sbjct: 495 LVTGYDIIFFWVARMVFSGIYNMGEVPFKHVYIHGLVRDAEGRKMSKSLGNGVDPLDVID 554

Query: 324 DFGTDALRFTLALGTAGQFD---KEESVLSLR--LPECWVVSKLHLLIDLVTTSYDKFYF 383
            FG DALRF L  G A   D   K E V + R    + W  S+  +L++L     DK+  
Sbjct: 555 TFGADALRFMLITGNAPGNDIRYKTEKVEAARNFANKIWNASRF-VLMNLDKEIMDKYK- 614

Query: 384 GDVARETYDFFWGDFADWYAYS--NSLCAQILLNLKLQQQLYLLSILMKVSLFDFFDMNA 443
                   D      AD +  S  NSL  ++  N+    + + L I  +  ++DF     
Sbjct: 615 --------DLEEYSLADRWILSRCNSLVREVTDNI----EKFELGIASQ-KVYDFMWNEF 674

Query: 444 CLIRYIEASKARLYQSEDNSVTTAAQAVLLYVFENILKILHPFMPFVTEALWQAIPNQKE 503
           C   YIE  K  +Y  ED      A  VL  V    L++LHP MP++TE ++Q +  + +
Sbjct: 675 C-DWYIELVKPVMY-GEDEKAKGIAYNVLYKVLTVGLQLLHPVMPYITEEIYQHLGGEYK 734

Query: 504 PLIVSSWP-----------KTSLPSYSNATRAIRNVRAEYSVEPVKRISATIVA---NPD 563
            + +S+WP           + ++     A ++IRNVRAE +V P K+    I     N  
Sbjct: 735 AIAISAWPTYEEKLKNETSENAMNQIIEAIKSIRNVRAEMNVPPSKKAKVMIFTEAENKA 794

Query: 564 VLQYMSEEKEVLALLSKLDLQVLSFAESLPEDANQSVHLVASEGLEAYLPLADMVDISAE 623
             +      E LA  S +    L   +  PE+A  SV    ++G E ++PL D++D++ E
Sbjct: 795 AFEMGEHYFEKLAYASSVSF--LKSKDEAPENAVSSV----TKGAELFMPLLDLIDVTKE 854

Query: 624 LPRLHKRLLKMDGEYKGIKARLGSPKFLEKAPEEIVRAAQEKADEMEEKINLTQKRIAFL 664
           + RL K   K+  E + +  +L +  F++KAPE +V A + K ++ ++ +   ++RIA L
Sbjct: 855 IERLSKEKDKLKAEIQRVDKKLSNKGFVDKAPESVVEAERVKGEKYKKMLEAVEERIAAL 881

		  

BLAST of Spo13623.1 vs. ExPASy Swiss-Prot
Match: SYV_BACLD (Valine--tRNA ligase OS=Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / NBRC 12200 / NCIMB 9375 / NRRL NRS-1264 / Gibson 46) GN=valS PE=3 SV=1)

HSP 1 Score: 373.2 bits (957), Expect = 6.100e-102
Identity = 224/604 (37.09%), Postives = 346/604 (57.28%), Query Frame = 1

		  

Query: 83  RGLDRFEAREKLWKDLTETGLAVKKQPHTLRVPRSQRGGEVRIIEPLVSKQWFVTMEALA 142
           +G+DRFE R+++ KDL E G+  K + HT  V  S+R G V  +EP +S QWFV M+ LA
Sbjct: 315 KGMDRFECRKQIVKDLQEEGVLFKIEEHTHSVGHSERSGAV--VEPYLSTQWFVQMQPLA 374

Query: 143 EKALHAVE-KGDLTIIPDRFEKIYNHWLTNIKDWCISRQLWWGHRIPVWYIVGRDCEEEY 202
           + A+   + +G +  +PDRFEK Y HW+ NI+DWCISRQLWWGHRIP WY   ++  E Y
Sbjct: 375 DAAIELQKSEGKVNFVPDRFEKTYLHWMENIRDWCISRQLWWGHRIPAWY--HKETGEIY 434

Query: 203 IVARSQEEALHKAQQKYGKDAEIY-QDPDVLDTWFSSALWPFSTLGWPDVSQEEYKMFYP 262
           +   + E            DAE + QD DVLDTWFSSALWPFST+GWPD+ +E++K +YP
Sbjct: 435 VGVEAPE------------DAENWEQDKDVLDTWFSSALWPFSTMGWPDIDEEDFKRYYP 494

Query: 263 TTMLETGHDILFFWVARMVMMGIEFTGNVPFRYVYLHGLIRDSQGRKMSKTLGNVIDPVD 322
           T +L TG+DI+FFWV+RM+  GIEFTG  PF+ V +HGLIRD QGRKMSK+LGN +DP+D
Sbjct: 495 TNVLVTGYDIIFFWVSRMIFQGIEFTGERPFKDVLIHGLIRDDQGRKMSKSLGNGVDPMD 554

Query: 323 TIKDFGTDALRFTLALGTA-GQ-----FDKEESVLSLRLPECWVVSKLHLLIDLVTTSYD 382
            I  +G D+LR+ LA G++ GQ     F+K ES  +    + W  S+   L+++   +Y+
Sbjct: 555 VIDKYGADSLRYFLATGSSPGQDLRFSFEKVESTWNF-ANKIWNASRF-ALMNMDGMTYE 614

Query: 383 KFYFGDVARETYDFFWGDFAD-WYAYSNSLCAQILLNLKLQQQLYLLSILMKVSLFDFFD 442
           +    D++ E         AD W     +   + +  L  + +   +   +   ++D F 
Sbjct: 615 EL---DLSGEK------SVADKWILTRLNETIETVTQLADKYEFGEVGRHLYNFIWDDF- 674

Query: 443 MNACLIRYIEASKARLYQSEDNSVTTAAQAVLLYVFENILKILHPFMPFVTEALWQAIPN 502
                  YIE +K  LY  ED +     +++L YV +  +++LHPFMPF+TE +WQ +P+
Sbjct: 675 ----CDWYIEMAKLPLY-GEDEAAKKTTRSILAYVLDQTMRLLHPFMPFLTEEIWQHLPH 734

Query: 503 QKEPLIVSSWPKTSLPSYSN------------ATRAIRNVRAEYSVEPVKRISATI-VAN 562
           + E + V+ WP+ ++  Y++              RA+RN+R+E +    K+I   I  + 
Sbjct: 735 EGESITVAKWPE-AVKEYTDTEAAADMKLLVEVIRAVRNIRSEVNTPLSKQIELYIKTST 794

Query: 563 PDVLQYMSEEKEVLALLSKLDLQVLSFAESLPEDANQSVHLVASEGLEAYLPLADMVDIS 622
           P++ + + E +  +   +   L  +       + A  +V      G E  LPL  +++I 
Sbjct: 795 PEIAERLEENRSYVERFTNPSLLQIGTDIQAVDKAMTAV----VSGAELILPLEGLINID 854

Query: 623 AELPRLHKRLLKMDGEYKGIKARLGSPKFLEKAPEEIVRAAQEKADEMEEKINLTQKRIA 665
            E+ RL K L K+  E + ++ +L +  F++KAP  ++   + K  +   K    +KRIA
Sbjct: 855 EEISRLQKELDKLTKEVERVQKKLSNEGFMKKAPAHVIEEERAKEADYTAKREAVEKRIA 880

		  

BLAST of Spo13623.1 vs. ExPASy Swiss-Prot
Match: SYV_BACHD (Valine--tRNA ligase OS=Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) GN=valS PE=3 SV=1)

HSP 1 Score: 370.5 bits (950), Expect = 4.000e-101
Identity = 229/609 (37.60%), Postives = 339/609 (55.67%), Query Frame = 1

		  

Query: 84  GLDRFEAREKLWKDLTETGLAVKKQPHTLRVPRSQRGGEVRIIEPLVSKQWFVTMEALAE 143
           G+DRFE R+++ KDL E G+  K + H   V  S+R G V  +EP +S QWFV M+ LAE
Sbjct: 316 GMDRFECRKQIVKDLQEQGVLFKIEEHVHSVGHSERSGAV--VEPYLSTQWFVKMKPLAE 375

Query: 144 KALHA-VEKGDLTIIPDRFEKIYNHWLTNIKDWCISRQLWWGHRIPVWYIVGRDCEEEYI 203
           +A+     +  +  +PDRFEK Y  W+ NI+DWCISRQLWWGHRIP WY   ++  E Y+
Sbjct: 376 QAIELQTTENKVNFVPDRFEKTYLRWMENIRDWCISRQLWWGHRIPAWY--HKETGEVYV 435

Query: 204 VARSQEEALHKAQQKYGKDAEIY-QDPDVLDTWFSSALWPFSTLGWPDVSQEEYKMFYPT 263
                    H+A Q    D + + QD DVLDTWFSSALWPFST+GWPD    +YK +Y T
Sbjct: 436 G--------HEAPQ----DIDNWKQDEDVLDTWFSSALWPFSTMGWPDEEAPDYKRYYST 495

Query: 264 TMLETGHDILFFWVARMVMMGIEFTGNVPFRYVYLHGLIRDSQGRKMSKTLGNVIDPVDT 323
             L TG+DI+FFWV+RM+  G+EFTG  PF+ V +HGL+RD++G+KMSK+LGN +DP++ 
Sbjct: 496 DALVTGYDIIFFWVSRMIFQGLEFTGQPPFKDVLIHGLVRDAEGQKMSKSLGNGVDPMEV 555

Query: 324 IKDFGTDALRFTLALGTAG------QFDKEESVLSLRLPECWVVSKLHLLIDLVTTSYDK 383
           I  +G DALRF LA G++        ++K ES  +    + W  S+   L+++   SYD+
Sbjct: 556 IDKYGADALRFFLATGSSPGNDLRFYWEKVESTWNFG-NKIWNASRF-ALMNMDGMSYDE 615

Query: 384 FYFGDVARETYDFFWGDFADWYAYSNSLCAQILLNLKLQQQLYLLSILMKVSLFDFFDMN 443
               D+  E                 S+  Q +L  +LQ+ +  ++ L  +  ++F ++ 
Sbjct: 616 I---DLTGE----------------KSIADQWILT-RLQETIETVTRL--IDAYEFGEVG 675

Query: 444 ACLIR---------YIEASKARLYQSEDNSVTTAAQAVLLYVFENILKILHPFMPFVTEA 503
             L           YIE +K  LY  ED       ++VL YV +  +++LHP MPF+TE 
Sbjct: 676 RHLYNFIWDDLCDWYIEMAKLPLY-GEDEVAKKTTRSVLAYVLDQTMRLLHPLMPFITEE 735

Query: 504 LWQAIPNQKEPLIVSSWPKTS-----------LPSYSNATRAIRNVRAEYSVEPVKRISA 563
           +WQ +P++ E + V+SWP  S           +    +  R++RN RAE +V   K I  
Sbjct: 736 IWQHLPHEGESITVASWPVKSEEFTFEQAMGDMELLKDIIRSVRNTRAELNVPMSKEIEL 795

Query: 564 TIVA-NPDVLQYMSEEKEVLALLSKLDLQVLSFAESLPEDANQSVHLVASEGLEAYLPLA 623
            I A N DVL  +   K  +         V+  A   PE +  +V      G+E YLPLA
Sbjct: 796 HIQAKNEDVLTQLERGKHYIEKFCNPSTLVMGTAIEKPEKSMSNV----LSGVELYLPLA 855

Query: 624 DMVDISAELPRLHKRLLKMDGEYKGIKARLGSPKFLEKAPEEIVRAAQEKADEMEEKINL 664
            ++D+  E+ RL K   K++ E + ++ +L +  FL KAPE+++   ++K  +  EK   
Sbjct: 856 GLLDLEEEIARLEKEENKLEKEVERVQKKLSNQGFLAKAPEKVIEEERKKEADYLEKRAA 879

		  

BLAST of Spo13623.1 vs. TAIR (Arabidopsis)
Match: AT5G16715.1 (ATP binding;valine-tRNA ligases;aminoacyl-tRNA ligases;nucleotide binding;ATP binding;aminoacyl-tRNA ligases)

HSP 1 Score: 718.8 bits (1854), Expect = 3.400e-207
Identity = 389/601 (64.73%), Postives = 453/601 (75.37%), Query Frame = 1

		  

Query: 84  GLDRFEAREKLWKDLTETGLAVKKQPHTLRVPRSQRGGEVRIIEPLVSKQWFVTMEALAE 143
           GLDRFE REKLW DL E GLAVKK+PHTLRVPRSQRGGEV  IEPLVSKQWFV M+ LAE
Sbjct: 377 GLDRFEVREKLWADLEEIGLAVKKEPHTLRVPRSQRGGEV--IEPLVSKQWFVHMDPLAE 436

Query: 144 KALHAVEKGDLTIIPDRFEKIYNHWLTNIKDWCISRQLWWGHRIPVWYIVGRDCEEEYIV 203
           KAL AVE  +LTIIP+RFEKIYNHWLTNIKDWCISRQLWWGHRIPVWY+VG+DCEE+YIV
Sbjct: 437 KALLAVENKELTIIPERFEKIYNHWLTNIKDWCISRQLWWGHRIPVWYVVGKDCEEDYIV 496

Query: 204 ARSQEEALHKAQQKYGKDAEIYQDPDVLDTWFSSALWPFSTLGWPDVSQEEYKMFYPTTM 263
           A+S EEAL KA +KYGKD EIYQDPDVLDTWFSS+LWPFSTLGWPDV+ +++  FYPT M
Sbjct: 497 AKSAEEALEKALEKYGKDVEIYQDPDVLDTWFSSSLWPFSTLGWPDVAAKDFNNFYPTNM 556

Query: 264 LETGHDILFFWVARMVMMGIEFTGNVPFRYVYLHGLIRDSQGRKMSKTLGNVIDPVDTIK 323
           LETGHDILFFWVARMVMMGIEFTG VPF +VYLHGLIRDSQGRKMSK+LGNVIDP+DTIK
Sbjct: 557 LETGHDILFFWVARMVMMGIEFTGTVPFSHVYLHGLIRDSQGRKMSKSLGNVIDPLDTIK 616

Query: 324 DFGTDALRFTLALGTAGQFDKEESVLSLRLPE-------CWVVSK--LHLLIDLVTTSYD 383
           DFGTDALRFT+ALGTAGQ   + ++ + RL          W   K  LH L  L  TS  
Sbjct: 617 DFGTDALRFTIALGTAGQ---DLNLSTERLTANKAFTNKLWNAGKFVLHSLPSLSDTSAW 676

Query: 384 KFYFGDVARETYDFFWGDFADWYAYSNSLCAQILLNLKLQQQLYLLSILMKVSLFDFFDM 443
           +        +          + +A S      IL++        L    +    +DFF  
Sbjct: 677 ENLLDLKLDKEETLLSLPLPECWAVSK---LHILIDSVTASYEKLFFGDVGRETYDFF-W 736

Query: 444 NACLIRYIEASKARLYQSEDNSVTTAAQAVLLYVFENILKILHPFMPFVTEALWQAIPNQ 503
           +     YIEASK+RLY S  NSV+ A+QAVLLYVFENILK+LHPFMPFVTE LWQA+P +
Sbjct: 737 SDFADWYIEASKSRLYGSGGNSVSLASQAVLLYVFENILKLLHPFMPFVTEDLWQALPYR 796

Query: 504 KEPLIVSSWPKTSLP----------SYSNATRAIRNVRAEYSVEPVKRISATIVANPDVL 563
           KE LIVS WP+ SLP          +    TRAIRN RAEYSVEPVKRISA++V + +V+
Sbjct: 797 KEALIVSPWPQNSLPRNVESIKRFENLQALTRAIRNARAEYSVEPVKRISASVVGSAEVI 856

Query: 564 QYMSEEKEVLALLSKLDLQVLSFAESLPEDANQSVHLVASEGLEAYLPLADMVDISAELP 623
           +Y+S+EKEVLALLS+LDL  + F+ + P DAN SVHLVASEGLEAYLPLA MVDIS+E+ 
Sbjct: 857 EYISKEKEVLALLSRLDLNNVHFSNAPPGDANLSVHLVASEGLEAYLPLAAMVDISSEVQ 916

Query: 624 RLHKRLLKMDGEYKGIKARLGSPKFLEKAPEEIVRAAQEKADEMEEKINLTQKRIAFLES 666
           R+ KRL KM  EY  +  RL SPKF+EKAPEE+VR  +E+ +E+EEKI LT+ R+ FL+S
Sbjct: 917 RISKRLSKMQTEYDALITRLSSPKFVEKAPEEVVRGVKEQVEELEEKIKLTKARLDFLKS 968

		  

BLAST of Spo13623.1 vs. TAIR (Arabidopsis)
Match: AT1G14610.1 (valyl-tRNA synthetase / valine--tRNA ligase (VALRS))

HSP 1 Score: 276.9 bits (707), Expect = 3.400e-74
Identity = 210/658 (31.91%), Postives = 321/658 (48.78%), Query Frame = 1

		  

Query: 84   GLDRFEAREKLWKDLTETGLAVKKQPHTLRVPRSQRGGEVRIIEPLVSKQWFVTMEALAE 143
            G+ RF ARE + + L + GL    + + +R+    R  +V  IEP++  QW+V    + +
Sbjct: 463  GMPRFAAREAVVEALQKQGLYRGAKNNEMRLGLCSRTNDV--IEPMIKPQWYVNCSMIGK 522

Query: 144  KALHAV---EKGDLTIIPDRFEKIYNHWLTNIKDWCISRQLWWGHRIPVWYIVGRDCE-- 203
            +AL      E   L  +P ++   +  WL NI+DWCISRQLWWGHRIP WY    + +  
Sbjct: 523  EALDVAITDENKKLEFVPKQYTAEWRRWLENIRDWCISRQLWWGHRIPAWYATLEEDQLK 582

Query: 204  ------EEYIVARSQEEALHKAQQKY-GKDAEIYQDPDVLDTWFSSALWPFSTLGWPDVS 263
                  + ++VAR++++A  +A QK+ GK  E+ +DPDVLDTWFSS L+P S LGWPDV+
Sbjct: 583  EVGAYSDHWVVARTEDDAREEAAQKFLGKKFELTRDPDVLDTWFSSGLFPLSVLGWPDVT 642

Query: 264  QEEYKMFYPTTMLETGHDILFFWVARMVMMGIEFTGNVPFRYVYLHGLIRDSQGRKMSKT 323
             +++K FYPT++LETGHDILFFWVARMVMMG++  G VPF  VY H +IRD+ GRKMSK+
Sbjct: 643  -DDFKAFYPTSVLETGHDILFFWVARMVMMGMKLGGEVPFSKVYFHPMIRDAHGRKMSKS 702

Query: 324  LGNVIDPVDTIKDFGTDALRFTLALGTAGQFDKEESVLSLR---------LPECWVVSKL 383
            LGNVIDP++ I     + L   L     G  D +E +++           +PEC     L
Sbjct: 703  LGNVIDPLEVINGVTLEGLHKRL---EEGNLDPKEVIVAKEGQVKDFPNGIPECG-TDAL 762

Query: 384  HLLIDLVTTSYDKFYFGDVAR------------ETYDFFWGDFADWYAYSNSLCAQILLN 443
               +   T   DK    D+ R                F      D Y    +L  +    
Sbjct: 763  RFALVSYTAQSDKINL-DILRVVGYRQWCNKLWNAVRFAMMKLGDGYTPPQTLSPE---T 822

Query: 444  LKLQQQLYLLSILMK------VSL--FDFFDMNACLIR---------YIEASKARLYQSE 503
            +    Q ++LS+L K      VSL  F+F D    +           YIEA K   Y + 
Sbjct: 823  MPFSCQ-WILSVLNKAISKTVVSLDAFEFSDAANTIYAWWQYQFCDVYIEAIKP--YFAG 882

Query: 504  DN----SVTTAAQAVLLYVFENILKILHPFMPFVTEALWQAIPNQKE------------P 563
            DN    S    AQ  L    E  L++LHPFMPFVTE LWQ +P  K+            P
Sbjct: 883  DNPTFASERAHAQHALWISLETGLRLLHPFMPFVTEELWQRLPAPKDTERKASIMICDYP 942

Query: 564  LIVSSWPKTSLPSYSN----ATRAIRNVRAE-YSVEPVKRISATIVANPDVLQYMSEEKE 623
              + +W    + S  +      + +R +RA     +  +R+ A  +   +V   + +  E
Sbjct: 943  SAIENWSNEKVESEMDTVLATVKCMRALRAGLLEKQKNERLPAFALCENNVTSEIVKSHE 1002

Query: 624  VLALLSKLDLQVLSFAESLPEDANQSVHL--------VASEGLEAYLPLADMVDISAELP 663
                   L+++ L+   SL E  ++  H           +E L+ YL +   ++  AE  
Sbjct: 1003 -------LEIRTLANLSSL-EVVSKGQHAAPPGSSVETVNENLKVYLEVDGAINTEAEQE 1062

		  

BLAST of Spo13623.1 vs. TAIR (Arabidopsis)
Match: AT5G49030.1 (tRNA synthetase class I (I, L, M and V) family protein)

HSP 1 Score: 70.9 bits (172), Expect = 3.700e-12
Identity = 74/296 (25.00%), Postives = 121/296 (40.88%), Query Frame = 1

		  

Query: 216 QKY-GKDAEIYQDPDVLDTWFSSALWPFSTLGWPDVSQEEYKMFYPTTMLETGHDILFFW 275
           +KY  K A+  +  D +D WF S         W  V  +   + +P  +   G D    W
Sbjct: 637 EKYRDKAADYEKGTDTMDVWFDSGS------SWAGVLGKREGLSFPADVYLEGTDQHRGW 696

Query: 276 VARMVMMGIEFTGNVPFRYVYLHGLIRDSQGRKMSKTLGNVIDPVDTIKDFGTDALRFTL 335
               ++  I   G  P+  V  HG + D +G KMSK+LGNV+DP   I+           
Sbjct: 697 FQSSLLTSIATQGKAPYSAVITHGFVLDEKGMKMSKSLGNVVDPRLVIE----------- 756

Query: 336 ALGTAGQFDKEESVLSLRLPECWVVSKLHLLIDLVTTSYDKFYFGDVARE---TYDFFWG 395
                G+  K+       +   W VS +    D++          D+ R+   T  +  G
Sbjct: 757 ----GGKNSKDAPAYGADVMRLW-VSSVDYTGDVLIGPQILRQMSDIYRKLRGTLRYLLG 816

Query: 396 DFADWYAYSNSLCAQILLNLKLQQQLYLLSILMK-----VSLFDFFDMNACLIR------ 455
           +  DW    N++  Q L  +  Q  L+ L  ++K        + FF +   + R      
Sbjct: 817 NLHDW-RVDNAVPYQDLPIID-QHALFQLENVVKNIQECYENYQFFKIFQIIQRFTIVDL 876

Query: 456 ---YIEASKARLYQSEDNSVT-TAAQAVLLYVFENILKILHPFMPFVTEALWQAIP 493
              Y + +K RLY    +S T  + Q VL     +IL+++ P +P + E +WQ +P
Sbjct: 877 SNFYFDIAKDRLYTGGTSSFTRRSCQTVLSTHLLSILRVIAPIVPHLAEDVWQNLP 908

		  

BLAST of Spo13623.1 vs. TAIR (Arabidopsis)
Match: AT4G10320.1 (tRNA synthetase class I (I, L, M and V) family protein)

HSP 1 Score: 60.8 bits (146), Expect = 3.800e-9
Identity = 29/106 (27.36%), Postives = 54/106 (50.94%), Query Frame = 1

		  

Query: 229 DVLDTWFSSALWPFSTLGWPDVSQEEYKMFYPTTMLETGHDILFFWVARMVMMGIEFTGN 288
           DV D WF S   P++ + +P  ++E ++  +P   +  G D    W   ++++       
Sbjct: 554 DVFDCWFESGSMPYAYIHYPFENKELFEKNFPGDFVAEGLDQTRGWFYTLMVLSTALFEK 613

Query: 289 VPFRYVYLHGLIRDSQGRKMSKTLGNVIDPVDTIKDFGTDALRFTL 335
             F+ +  +GL+    G+KM+K L N   P++ I ++G DA+R  L
Sbjct: 614 PAFKNLICNGLVLAEDGKKMAKKLRNYPPPLEVIDEYGADAVRLYL 659

		  

BLAST of Spo13623.1 vs. TAIR (Arabidopsis)
Match: AT3G55400.1 (methionyl-tRNA synthetase / methionine--tRNA ligase / MetRS (cpMetRS))

HSP 1 Score: 51.6 bits (122), Expect = 2.300e-6
Identity = 35/133 (26.32%), Postives = 60/133 (45.11%), Query Frame = 1

		  

Query: 227 DPDVLDTWFSSALWPFSTLGWPDVSQE---EYKMFYPTTMLETGHDILFF----WVARMV 286
           D   +  WF + L   S L   +  Q         +P ++   G DIL F    W A ++
Sbjct: 285 DKQTIYVWFDALLGYISALTEDNKQQNLETAVSFGWPASLHLIGKDILRFHAVYWPAMLM 344

Query: 287 MMGIEFTGNVPFRYVYLHGLIRDSQGRKMSKTLGNVIDPVDTIKDFGTDALRF----TLA 346
             G+E       + V+ HG +    G KM K+LGN ++P + ++ FG DA+R+     + 
Sbjct: 345 SAGLELP-----KMVFGHGFLT-KDGMKMGKSLGNTLEPFELVQKFGPDAVRYFFLREVE 404

Query: 347 LGTAGQFDKEESV 349
            G  G + ++  +
Sbjct: 405 FGNDGDYSEDRFI 411