Spo14816.1 (mRNA) | SpinachBase

Overview


Name	Spo14816.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	NADH-ubiquinone oxidoreductase chain 4 (1.6.5.3)
Location	chr5 : 52729204 .. 52730216 (-)
Sequence length	465

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAAAGCCCGGGTGAAAGCAAAAAGAAAAAGGTATGAAGTCGCTAAAGCGAAGATTTTGGAGAGAAGAAAAGTAGAAAGAGGGAAAGAAGTTTAGTGCTAGTTCTTGCTGATTTATTTATTTAGATTCTACATTGGATAAAGAATGAAAAAAAGAATCTAAATAAAGCTCAGGGGGGCGGGAGGCCCCACTACTTCTTCATTCAGGGGGGAGGAATCAATTTCCACTGGGCGAGAGGTACACCTAACTTACCTACCTACTCATCAATCACGGTATTCAGCTGACTTGCACTGATAGACCTTTATTGTATTAGAATTAGGCGCTCATCTTTTTACTGTTGTCAACTAATCTTTTCAATGTTCGATTTTGCTCTATGTTAGAACATTTCTGTGAATGCTATTCTGATCTAAGTGGTCTTATTCTGTGTCCCGTGCTAGGAAGCATTACTCTTCTTTTCATTCCAAATTCAAGAATACGATCGATACGATTGATTGGTCTGTGCGCTTCTCTTATTACTTTTTTGTATTCTCCTGTTCTTCGGATACAATTTGATCCTTCTACGGCCAAATCTCAATTTGTGGAAAGCCTTCGATGGCTTCCTTATGAAAACATCAATTTTTATTTGGGTATAGACGGTATCTCTTTATTCTTCGTGATATTGACCACATTTTTGATCCCTATTTGCATTTTAGTGGGTTGGTCTGGTATGAGAAGTTATGGGAAAGAGTATATTACAGCATCTCTAATTTTAGAATTTCTAATGATCGCCGTGTTCTGCATGCTGGATCTTCTACTATTCTATGTTCTTTTCGAAAGCGTGCCAATCCCTATGTTGTGCGGAGCTGAGCATCTTCTATTCGCTAGGATAAATCCTCTGCAGGGGCCTTGTGCAGTAAACCCCCTACGGGCGGTCGTCCGTCGTCGTAAAGTAGTCTCCGCGAAGCTTTCGGGAAGAGGGGTAGTCTTGTGTGTAAGCATAGCATTTCTGGTCGAACCCGCCCAACTTAACTAA

mRNA sequence

ATGAAAAGCCCGGGTGAAAGCAAAAAGAAAAAGATTCTACATTGGATAAAGAATGAAAAAAAGAATCTAAATAAAGCTCAGGGGGGCGGGAGGCCCCACTACTTCTTCATTCAGGGGGGAGGAATCAATTTCCACTGGGCGAGAGTGGGTTGGTCTGGTATGAGAAGTTATGGGAAAGAGTATATTACAGCATCTCTAATTTTAGAATTTCTAATGATCGCCGTGTTCTGCATGCTGGATCTTCTACTATTCTATGTTCTTTTCGAAAGCGTGCCAATCCCTATGTTGTGCGGAGCTGAGCATCTTCTATTCGCTAGGATAAATCCTCTGCAGGGGCCTTGTGCAGTAAACCCCCTACGGGCGGTCGTCCGTCGTCGTAAAGTAGTCTCCGCGAAGCTTTCGGGAAGAGGGGTAGTCTTGTGTGTAAGCATAGCATTTCTGGTCGAACCCGCCCAACTTAACTAA

Coding sequence (CDS)

ATGAAAAGCCCGGGTGAAAGCAAAAAGAAAAAGATTCTACATTGGATAAAGAATGAAAAAAAGAATCTAAATAAAGCTCAGGGGGGCGGGAGGCCCCACTACTTCTTCATTCAGGGGGGAGGAATCAATTTCCACTGGGCGAGAGTGGGTTGGTCTGGTATGAGAAGTTATGGGAAAGAGTATATTACAGCATCTCTAATTTTAGAATTTCTAATGATCGCCGTGTTCTGCATGCTGGATCTTCTACTATTCTATGTTCTTTTCGAAAGCGTGCCAATCCCTATGTTGTGCGGAGCTGAGCATCTTCTATTCGCTAGGATAAATCCTCTGCAGGGGCCTTGTGCAGTAAACCCCCTACGGGCGGTCGTCCGTCGTCGTAAAGTAGTCTCCGCGAAGCTTTCGGGAAGAGGGGTAGTCTTGTGTGTAAGCATAGCATTTCTGGTCGAACCCGCCCAACTTAACTAA

Protein sequence

MKSPGESKKKKILHWIKNEKKNLNKAQGGGRPHYFFIQGGGINFHWARVGWSGMRSYGKEYITASLILEFLMIAVFCMLDLLLFYVLFESVPIPMLCGAEHLLFARINPLQGPCAVNPLRAVVRRRKVVSAKLSGRGVVLCVSIAFLVEPAQLN

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo14816	Spo14816	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo14816.1	Spo14816.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo14816.1.CDS.3	Spo14816.1.CDS.3	CDS
Spo14816.1.CDS.2	Spo14816.1.CDS.2	CDS
Spo14816.1.CDS.1	Spo14816.1.CDS.1	CDS

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo14816.1.exon.3	Spo14816.1.exon.3	exon
Spo14816.1.exon.2	Spo14816.1.exon.2	exon
Spo14816.1.exon.1	Spo14816.1.exon.1	exon

Homology

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BLAST of Spo14816.1 vs. NCBI nr
Match: gi|323435103|ref|YP_004222321.1| (hypothetical protein BevumaM_p086 (mitochondrion) [Beta vulgaris subsp. maritima])

HSP 1 Score: 110.2 bits (274), Expect = 3.400e-21
Identity = 57/59 (96.61%), Postives = 57/59 (96.61%), Query Frame = 1

		  
Query: 49  VGWSGMRSYGKEYITASLILEFLMIAVFCMLDLLLFYVLFESVPIPMLCGAEHLLFARI 108
           VGWSGMRSYGKEYITASLI EFLMIAVFCMLDLLLFYVL ESVPIPMLCGAEHLLFARI
Sbjct: 107 VGWSGMRSYGKEYITASLIREFLMIAVFCMLDLLLFYVLSESVPIPMLCGAEHLLFARI 165

[Close]

BLAST of Spo14816.1 vs. NCBI nr
Match: gi|586829798|gb|AHJ81038.1| (orf175 (mitochondrion) [Panax ginseng])

HSP 1 Score: 99.4 bits (246), Expect = 5.900e-18
Identity = 54/59 (91.53%), Postives = 54/59 (91.53%), Query Frame = 1

		  
Query: 49  VGWSGMRSYGKEYITASLILEFLMIAVFCMLDLLLFYVLFESVPIPMLCGAEHLLFARI 108
           VGWSGMRSYGKEYITASLI EFLMIAVF MLD LLFYVL ESVPIPMLCGAEHLLFA I
Sbjct: 107 VGWSGMRSYGKEYITASLIREFLMIAVFRMLDPLLFYVLPESVPIPMLCGAEHLLFAGI 165

[Close]

BLAST of Spo14816.1 vs. NCBI nr
Match: gi|992272480|gb|KXG32594.1| (hypothetical protein SORBI_003G173200 [Sorghum bicolor])

HSP 1 Score: 97.8 bits (242), Expect = 1.700e-17
Identity = 52/59 (88.14%), Postives = 53/59 (89.83%), Query Frame = 1

		  
Query: 49  VGWSGMRSYGKEYITASLILEFLMIAVFCMLDLLLFYVLFESVPIPMLCGAEHLLFARI 108
           VGWSGMRS+GKEYITA LI EFLMIAV CMLD LLFYVL ESVPIPMLCGAEHLLFA I
Sbjct: 48  VGWSGMRSFGKEYITAFLIREFLMIAVSCMLDPLLFYVLSESVPIPMLCGAEHLLFAGI 106

[Close]

BLAST of Spo14816.1 vs. NCBI nr
Match: gi|944221342|gb|KQK85789.1| (hypothetical protein SETIT_020767mg, partial [Setaria italica])

HSP 1 Score: 97.8 bits (242), Expect = 1.700e-17
Identity = 52/59 (88.14%), Postives = 53/59 (89.83%), Query Frame = 1

		  
Query: 49  VGWSGMRSYGKEYITASLILEFLMIAVFCMLDLLLFYVLFESVPIPMLCGAEHLLFARI 108
           VGWSGMRS+GKEYITA LI EFLMIAV CMLD LLFYVL ESVPIPMLCGAEHLLFA I
Sbjct: 107 VGWSGMRSFGKEYITAFLIREFLMIAVSCMLDPLLFYVLSESVPIPMLCGAEHLLFAGI 165

[Close]

BLAST of Spo14816.1 vs. NCBI nr
Match: gi|242053077|ref|XP_002455684.1| (hypothetical protein SORBIDRAFT_03g020225, partial [Sorghum bicolor])

HSP 1 Score: 97.8 bits (242), Expect = 1.700e-17
Identity = 52/59 (88.14%), Postives = 53/59 (89.83%), Query Frame = 1

		  
Query: 49  VGWSGMRSYGKEYITASLILEFLMIAVFCMLDLLLFYVLFESVPIPMLCGAEHLLFARI 108
           VGWSGMRS+GKEYITA LI EFLMIAV CMLD LLFYVL ESVPIPMLCGAEHLLFA I
Sbjct: 84  VGWSGMRSFGKEYITAFLIREFLMIAVSCMLDPLLFYVLSESVPIPMLCGAEHLLFAGI 142

[Close]

BLAST of Spo14816.1 vs. UniProtKB/TrEMBL
Match: E6ZDW4_BETVM (Putative uncharacterized protein orf175 OS=Beta vulgaris subsp. maritima GN=orf175 PE=4 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 2.300e-21
Identity = 57/59 (96.61%), Postives = 57/59 (96.61%), Query Frame = 1

		  
Query: 49  VGWSGMRSYGKEYITASLILEFLMIAVFCMLDLLLFYVLFESVPIPMLCGAEHLLFARI 108
           VGWSGMRSYGKEYITASLI EFLMIAVFCMLDLLLFYVL ESVPIPMLCGAEHLLFARI
Sbjct: 107 VGWSGMRSYGKEYITASLIREFLMIAVFCMLDLLLFYVLSESVPIPMLCGAEHLLFARI 165

[Close]

BLAST of Spo14816.1 vs. UniProtKB/TrEMBL
Match: G2XLU3_BETMA (Uncharacterized protein OS=Beta macrocarpa GN=orf175 PE=4 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 2.300e-21
Identity = 57/59 (96.61%), Postives = 57/59 (96.61%), Query Frame = 1

		  
Query: 49  VGWSGMRSYGKEYITASLILEFLMIAVFCMLDLLLFYVLFESVPIPMLCGAEHLLFARI 108
           VGWSGMRSYGKEYITASLI EFLMIAVFCMLDLLLFYVL ESVPIPMLCGAEHLLFARI
Sbjct: 107 VGWSGMRSYGKEYITASLIREFLMIAVFCMLDLLLFYVLSESVPIPMLCGAEHLLFARI 165

[Close]

BLAST of Spo14816.1 vs. UniProtKB/TrEMBL
Match: A0A096XFA7_PANGI (Orf175 OS=Panax ginseng GN=orf175 PE=4 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 4.100e-18
Identity = 54/59 (91.53%), Postives = 54/59 (91.53%), Query Frame = 1

		  
Query: 49  VGWSGMRSYGKEYITASLILEFLMIAVFCMLDLLLFYVLFESVPIPMLCGAEHLLFARI 108
           VGWSGMRSYGKEYITASLI EFLMIAVF MLD LLFYVL ESVPIPMLCGAEHLLFA I
Sbjct: 107 VGWSGMRSYGKEYITASLIREFLMIAVFRMLDPLLFYVLPESVPIPMLCGAEHLLFAGI 165

[Close]

BLAST of Spo14816.1 vs. UniProtKB/TrEMBL
Match: W5AVM2_WHEAT (Uncharacterized protein OS=Triticum aestivum PE=4 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 1.200e-17
Identity = 52/59 (88.14%), Postives = 53/59 (89.83%), Query Frame = 1

		  
Query: 49  VGWSGMRSYGKEYITASLILEFLMIAVFCMLDLLLFYVLFESVPIPMLCGAEHLLFARI 108
           VGWSGMRS+GKEYITA LI EFLMIAV CMLD LLFYVL ESVPIPMLCGAEHLLFA I
Sbjct: 24  VGWSGMRSFGKEYITAFLIREFLMIAVSCMLDPLLFYVLSESVPIPMLCGAEHLLFAGI 82

[Close]

BLAST of Spo14816.1 vs. UniProtKB/TrEMBL
Match: W5DTC0_WHEAT (Uncharacterized protein OS=Triticum aestivum PE=4 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 1.200e-17
Identity = 52/59 (88.14%), Postives = 53/59 (89.83%), Query Frame = 1

		  
Query: 49  VGWSGMRSYGKEYITASLILEFLMIAVFCMLDLLLFYVLFESVPIPMLCGAEHLLFARI 108
           VGWSGMRS+GKEYITA LI EFLMIAV CMLD LLFYVL ESVPIPMLCGAEHLLFA I
Sbjct: 107 VGWSGMRSFGKEYITAFLIREFLMIAVSCMLDPLLFYVLSESVPIPMLCGAEHLLFAGI 165

[Close]

BLAST of Spo14816.1 vs. ExPASy Swiss-Prot
Match: NU4M_WHEAT (NADH-ubiquinone oxidoreductase chain 4 OS=Triticum aestivum GN=ND4 PE=2 SV=2)

HSP 1 Score: 78.2 bits (191), Expect = 8.900e-14
Identity = 41/47 (87.23%), Postives = 42/47 (89.36%), Query Frame = 1

		  
Query: 49  VGWSGMRSYGKEYITASLILEFLMIAVFCMLDLLLFYVLFESVPIPM 96
           VGWSGMRS+GKEYI A LI EFLMIAVFCMLDLLLFYV FESV IPM
Sbjct: 107 VGWSGMRSFGKEYIIAFLICEFLMIAVFCMLDLLLFYVFFESVLIPM 153

[Close]

BLAST of Spo14816.1 vs. ExPASy Swiss-Prot
Match: NU4M_BRACM (NADH-ubiquinone oxidoreductase chain 4 OS=Brassica campestris GN=ND4 PE=3 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 2.000e-13
Identity = 42/47 (89.36%), Postives = 42/47 (89.36%), Query Frame = 1

		  
Query: 49  VGWSGMRSYGKEYITASLILEFLMIAVFCMLDLLLFYVLFESVPIPM 96
           VGWSGMRSYGKEYITA LI EFLMIAVF MLDLLLFYV  ESVPIPM
Sbjct: 107 VGWSGMRSYGKEYITAFLIREFLMIAVFRMLDLLLFYVFPESVPIPM 153

[Close]

BLAST of Spo14816.1 vs. ExPASy Swiss-Prot
Match: NU4M_ARATH (NADH-ubiquinone oxidoreductase chain 4 OS=Arabidopsis thaliana GN=ND4 PE=2 SV=2)

HSP 1 Score: 75.9 bits (185), Expect = 4.400e-13
Identity = 41/47 (87.23%), Postives = 41/47 (87.23%), Query Frame = 1

		  
Query: 49  VGWSGMRSYGKEYITASLILEFLMIAVFCMLDLLLFYVLFESVPIPM 96
           VGWSGMRSYGKEYI A LI EFLMIAVFCMLDLLLFYV  ESV IPM
Sbjct: 107 VGWSGMRSYGKEYIIAFLICEFLMIAVFCMLDLLLFYVFPESVLIPM 153

[Close]

BLAST of Spo14816.1 vs. ExPASy Swiss-Prot
Match: NU4M_MARPO (NADH-ubiquinone oxidoreductase chain 4 OS=Marchantia polymorpha GN=ND4 PE=3 SV=2)

HSP 1 Score: 53.9 bits (128), Expect = 1.800e-6
Identity = 29/47 (61.70%), Postives = 35/47 (74.47%), Query Frame = 1

		  
Query: 49  VGWSGMRSYGKEYITASLILEFLMIAVFCMLDLLLFYVLFESVPIPM 96
           VG+  ++SY KEY+ A  I E  +IAVFC LDLL+FYV FESV IPM
Sbjct: 107 VGFYSVKSYKKEYMIAFFICESFLIAVFCSLDLLIFYVFFESVLIPM 153

[Close]

BLAST of Spo14816.1 vs. ExPASy Swiss-Prot
Match: NU4M_PROWI (NADH-ubiquinone oxidoreductase chain 4 OS=Prototheca wickerhamii GN=ND4 PE=3 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 3.100e-6
Identity = 28/47 (59.57%), Postives = 34/47 (72.34%), Query Frame = 1

		  
Query: 49  VGWSGMRSYGKEYITASLILEFLMIAVFCMLDLLLFYVLFESVPIPM 96
           V W+ +  Y KEY  A L+LE LM+ VF +LDLLLFY+ FESV IPM
Sbjct: 138 VSWNNIEVYVKEYCIAFLVLETLMLTVFSVLDLLLFYIFFESVLIPM 184

[Close]

BLAST of Spo14816.1 vs. TAIR (Arabidopsis)
Match: ATMG00580.1 (NADH dehydrogenase subunit 4)

HSP 1 Score: 77.0 bits (188), Expect = 1.100e-14
Identity = 42/47 (89.36%), Postives = 42/47 (89.36%), Query Frame = 1

		  
Query: 49  VGWSGMRSYGKEYITASLILEFLMIAVFCMLDLLLFYVLFESVPIPM 96
           VGWSGMRSYGKEYITA LI EFLMIAVF MLDLLLFYV  ESVPIPM
Sbjct: 107 VGWSGMRSYGKEYITAFLIREFLMIAVFRMLDLLLFYVFPESVPIPM 153

The following BLAST results are available for this feature:

BLAST of Spo14816.1 vs. NCBI nr
BLAST of Spo14816.1 vs. UniProtKB/TrEMBL
BLAST of Spo14816.1 vs. ExPASy Swiss-Prot
BLAST of Spo14816.1 vs. TAIR (Arabidopsis)

BLAST of Spo14816.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5

Position :

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x 1


Match Name	E-value	Identity	Description
gi\|323435103\|ref\|YP_004222321.1\|	3.4e-21	96.6	hypothetical protein BevumaM_p...	[more]
gi\|586829798\|gb\|AHJ81038.1\|	5.9e-18	91.5	orf175 (mitochondrion) [Panax ...	[more]
gi\|992272480\|gb\|KXG32594.1\|	1.7e-17	88.1	hypothetical protein SORBI_003...	[more]
gi\|944221342\|gb\|KQK85789.1\|	1.7e-17	88.1	hypothetical protein SETIT_020...	[more]
gi\|242053077\|ref\|XP_002455684.1\|	1.7e-17	88.1	hypothetical protein SORBIDRAF...	[more]

BLAST of Spo14816.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5

Position :

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x 1


Match Name	E-value	Identity	Description
E6ZDW4_BETVM	2.3e-21	96.6	Putative uncharacterized prote...	[more]
G2XLU3_BETMA	2.3e-21	96.6	Uncharacterized protein OS=Bet...	[more]
A0A096XFA7_PANGI	4.1e-18	91.5	Orf175 OS=Panax ginseng GN=orf...	[more]
W5AVM2_WHEAT	1.2e-17	88.1	Uncharacterized protein OS=Tri...	[more]
W5DTC0_WHEAT	1.2e-17	88.1	Uncharacterized protein OS=Tri...	[more]

BLAST of Spo14816.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5

Position :

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x 1


Match Name	E-value	Identity	Description
NU4M_WHEAT	8.9e-14	87.2	NADH-ubiquinone oxidoreductase...	[more]
NU4M_BRACM	2.0e-13	89.3	NADH-ubiquinone oxidoreductase...	[more]
NU4M_ARATH	4.4e-13	87.2	NADH-ubiquinone oxidoreductase...	[more]
NU4M_MARPO	1.8e-6	61.7	NADH-ubiquinone oxidoreductase...	[more]
NU4M_PROWI	3.1e-6	59.5	NADH-ubiquinone oxidoreductase...	[more]

BLAST of Spo14816.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 1

Position :

Zoom :

x 1


Match Name	E-value	Identity	Description
ATMG00580.1	1.1e-14	89.3	NADH dehydrogenase subunit 4	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR22773	NADH DEHYDROGENASE	coord: 49..96 score: 1.3
None	No IPR available	PANTHER	PTHR22773:SF88	NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 4	coord: 49..96 score: 1.3

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006120	mitochondrial electron transport, NADH to ubiquinone
biological_process	GO:1902600	proton transmembrane transport
biological_process	GO:0006814	sodium ion transport
biological_process	GO:0006744	ubiquinone biosynthetic process
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0008137	NADH dehydrogenase (ubiquinone) activity