Spo15068.1 (mRNA) | SpinachBase

Overview


Name	Spo15068.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	Non-lysosomal glucosylceramidase (NLGase) (3.2.1.45)
Location	chr4 : 5213479 .. 5216985 (+)
Sequence length	1516

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTGGTGCACATATGATGTGCATTTCTATGCATCTTTTGCACTGCTTGATTTGTTTCCCAAAATTGAACTTAGTATTCAACGTGAATTCGCCAAAGCTGTCCTGTCTGAGGATGGTAGAAAAGTACGATTCCTAGCTGAAGGTAACTGGGGAATTCGCAAAGTAAGAGGAGCTGTTCCTCATGATCTGGGAACACATGACCCGTGGAAAGAGATGAATGCTTATAATATACACGATACAAGCAAATGGAAGGATTTGAACCCCAAGTTTGTACTCCAGGTCTACAGAGATTTCGCAGCAACAGGAGACATGGCATTTGGAATTGATGTTTGGCCTTCTGTTCGTGCTGCCATGGAGTATATGGACCAGTTTGACATGGATGGTGATGGCCTTATAGAGAATGATGGATTTCCAGATCAGACATATGATACATGGACAGTTCATGGAGTCAGTGCTTACTGTGGCTGTCTATGGCTTGCAGCACTTCAGGCTGCAGCAGCAATGGCTTTCCAATTAGGTGACGAGGAATTTGGTGAAAAATACAAGAGAAAATTTCAGATGGCCAAACATGCGTTTGAAGCCAAGCTGTGGAATGGCTCTTACTTCAATTATGACTCTGGATCAAGTAGTAACAGTAAATCTATTCAAGCTGATCAATTAGCTGGACAGTGGTACACAGCTTCGTCTGGGTTGCCTAATCTCTTCGATGACTTCAAAATCAAGAGCACCCTGCAGAAATTCTTTGATTACAATGTCATGAAAGTTAAAGGAGGCAGGGCAGGTGCTGTAAATGGGATACATCCCAACGGAAAGGTGGATGAGACCTGCATGCAATCACGTGAAATTTGGACTGGAGTTACTTATGCTGTGGCTGCTAAAATGATTCTTGCAGGGATGGAGGAGCAGGCCTTCTCAACTGCTGAAGGAATTTTCATTGCTGGATGGTCTGAAGACGGATATGGGTAAGGTGTATTTTTATTTTACTGTAGCGAATCTTCTTTGCTTACTTATTCTCTCTCCTAAGAGTGTCATTTGTCATTATACTCTTACCAATCACAGACATTATTTTGGTGGCCGAAGAGATTTTCTGACTTGCACAATCTCACCTTTTCCCTCGTCTATTTTGTTACTTGTCAGTTGTCACTGTCACTTCTGCATATTTTTATTTATTTTTCCGTCTACAATCTAGCACCCTTCGTCCGATGCAACCATAAATATTGGCTTTCTGGAGGATATTTTTCATTTGTGGTTTGCGGTTCTACACATAATATTTTTAAGAAGTTGTCACCTATGTTTGCTTAGTTAATGATGATTAGAACTTAGTAGGAGTGGACATGAACGTTCTCTTCACACTGACCTTGTCCTCAAAATCTTCTTAGGGGCACAGTGGGAGGGATTGGTAATTAAAGCTTATCGTGATTGAAGCGACAATCTAATGATATAGTTCCGGCCATCCGGGTGACTAAAATAAAATAAATAAAAGTCTAGATCTCTGAAAACAAATTTAGAGGATTTTGAATTTGAAGTGTCCAAGCAATGTGAAAAAAGGTCAAGTGATCACTGAAGAGCACTAAAAAGTAAATCAAGCACAAGTGAAACTCAATAATCAATATTCCACTCTTCTCCTAATATTTACATCGTATCGCTTGACATATCTGAAAACAATAACCCGAACATAATTCCAGACTACACCCTAATAGAAATAAATAAACTAAATACTACTAGATAAATAAGTAAATAAAACTCACTCAAACTTTAAGGTCATGTTTCGTTTACCTTTATGTCACTTATTTCAAGACCAATAAGTTAAAATAAGTTCAGATAATGGTTGATAAGTTCAGATAATAATTGATAAAAGGTCAGATAAGGGTCGATAAGGATTACCCAAGTACAATTTACAACTAAAATAAGTTATAGTAAGTTCAGATAAGTGAAATAAGGTGAATAGATCGGACCTTAACTCCTACTACGGATACACGTAACTAGTAGATATTAGAAATCCTAAATAAGTTATGCAGCTTTGTCCTAAATACAATCTTCTAAGAACTCCTTTGAATGTTAATACTGAGATGCCACATAATATTTTGTGTTTTGTTTATTTAATGGGCTTTATATAAGTATATACACACACAAACCACAAAACAAGTCAGACCGAAATATCAAAAAATCGATCTTAATGGCTAATCTTTTATCAGCCCAATGCTTATGCATCATCTAATGATAGGAACTGCATGCATTCTCATTTTTCAGTTAAAGATGGAACAACTATTATACACCAAATGGTCTTTCCATCTTATGATTAGTTGATCATTAGCTCATATGTATAGAACTTCTGCTCCCCTAAAGTTTAGGGTATGGGTCCAATAATCCAATTTATTTAAACCCTATGAGTTTAGGTAGGGTGTAAGTGTTCTCAGATAGGTACATTCCGTCCATATTCAGTTCCAAAGTCCAAACCCTGCTCAACCCAACCCAACCCAACCCAAACCGGCCCTATGTACCTGTCTTAAAAAATAAAGTAAACTAGAAATAGATAGAAAAAGACGACCTAAGTTCAGAAAAACAAAACAAAGCTATGGTTGGATTTTTGTGGCCATTTTCTTTTTTGTGCATTATGTGCTTGTAGAAAATTGGACGACATAAAACTGGATAGTAGTAAGTTTTGAACCTAGGCTGGACTCGTACAGTCATACACCCATACTCTATGCGTTCAGGTAACCTTCTTATTTCTTTGACCTATGGGTCCAAGTCTTACTAGGACTCATCCATTGCCAACCCTAGTTCATACACTGGGATGTCATTCTTTGTTGGTTTGTGTTTTAGAACCTTTTTGTGGGTGTACCAGGTTGTGTTTTATCTGTAAAACCGTCTTTCAGCAACATCCAAGTTTCCTTCTAAGGGGTGGTATTATTTAGTAATTTAATTTTTTCCATTGTTTTTTCTCATTTTGTAGATATTGGTTTCAAACCCCGGAGGCTTGGACAATGGATGGCCATTTCCGTTCTCTAATTTACATGAGGCCGTTGGCAATTTGGGAAATGCAGTGGGCATTATCCATGCCTACAGCAGTTATTGAGGCGCCTAAGATTAACATAATGGACAGGTCTGATTTGCCTGCTTTAAGTTCAAGATCCACTCACAACGAGGCAGGTGGAAGAAAGGCCTCAAGAAAAATGAAGTGTTTCGGGAACTCAGTGTTTAGTTGGGCATGTTAAATGTGTGTATATTGCTTTTTTCTAGCAGAAATATTAGTTGCTAGATCCTTCAGTCTTCAATCCTTACACCTATTAATCCTATTAATCCTCCCTTACCTCTACCAGCCTTTGTTGTCTCAGGATGGCAGGGGTATGGGTCAGAGCATGATTTTTTATACAGGGATTAAACCCTAAAGACTGAGTTCCTCTGTTTCATAAAAATTGTAACGCTTTTACTTTTTGTAATACACCGACAATTCTCCATTTTCTAGAATAACCTTTTTAATAAATATAAATATACAGATTTATCAAA

mRNA sequence

ATGTGGTGCACATATGATGTGCATTTCTATGCATCTTTTGCACTGCTTGATTTGTTTCCCAAAATTGAACTTAGTATTCAACGTGAATTCGCCAAAGCTGTCCTGTCTGAGGATGGTAGAAAAGTACGATTCCTAGCTGAAGGTAACTGGGGAATTCGCAAAGTAAGAGGAGCTGTTCCTCATGATCTGGGAACACATGACCCGTGGAAAGAGATGAATGCTTATAATATACACGATACAAGCAAATGGAAGGATTTGAACCCCAAGTTTGTACTCCAGGTCTACAGAGATTTCGCAGCAACAGGAGACATGGCATTTGGAATTGATGTTTGGCCTTCTGTTCGTGCTGCCATGGAGTATATGGACCAGTTTGACATGGATGGTGATGGCCTTATAGAGAATGATGGATTTCCAGATCAGACATATGATACATGGACAGTTCATGGAGTCAGTGCTTACTGTGGCTGTCTATGGCTTGCAGCACTTCAGGCTGCAGCAGCAATGGCTTTCCAATTAGGTGACGAGGAATTTGGTGAAAAATACAAGAGAAAATTTCAGATGGCCAAACATGCGTTTGAAGCCAAGCTGTGGAATGGCTCTTACTTCAATTATGACTCTGGATCAAGTAGTAACAGTAAATCTATTCAAGCTGATCAATTAGCTGGACAGTGGTACACAGCTTCGTCTGGGTTGCCTAATCTCTTCGATGACTTCAAAATCAAGAGCACCCTGCAGAAATTCTTTGATTACAATGTCATGAAAGTTAAAGGAGGCAGGGCAGGTGCTGTAAATGGGATACATCCCAACGGAAAGGTGGATGAGACCTGCATGCAATCACGTGAAATTTGGACTGGAGTTACTTATGCTGTGGCTGCTAAAATGATTCTTGCAGGGATGGAGGAGCAGGCCTTCTCAACTGCTGAAGGAATTTTCATTGCTGGATGGTCTGAAGACGGATATGGATATTGGTTTCAAACCCCGGAGGCTTGGACAATGGATGGCCATTTCCGTTCTCTAATTTACATGAGGCCGTTGGCAATTTGGGAAATGCAGTGGGCATTATCCATGCCTACAGCAGTTATTGAGGCGCCTAAGATTAACATAATGGACAGGTCTGATTTGCCTGCTTTAAGTTCAAGATCCACTCACAACGAGGCAGGTGGAAGAAAGGCCTCAAGAAAAATGAAGTGTTTCGGGAACTCAGTGTTTAGTTGGGCATGTTAAATGTGTGTATATTGCTTTTTTCTAGCAGAAATATTAGTTGCTAGATCCTTCAGTCTTCAATCCTTACACCTATTAATCCTATTAATCCTCCCTTACCTCTACCAGCCTTTGTTGTCTCAGGATGGCAGGGGTATGGGTCAGAGCATGATTTTTTATACAGGGATTAAACCCTAAAGACTGAGTTCCTCTGTTTCATAAAAATTGTAACGCTTTTACTTTTTGTAATACACCGACAATTCTCCATTTTCTAGAATAACCTTTTTAATAAATATAAATATACAGATTTATCAAA

Coding sequence (CDS)

ATGTGGTGCACATATGATGTGCATTTCTATGCATCTTTTGCACTGCTTGATTTGTTTCCCAAAATTGAACTTAGTATTCAACGTGAATTCGCCAAAGCTGTCCTGTCTGAGGATGGTAGAAAAGTACGATTCCTAGCTGAAGGTAACTGGGGAATTCGCAAAGTAAGAGGAGCTGTTCCTCATGATCTGGGAACACATGACCCGTGGAAAGAGATGAATGCTTATAATATACACGATACAAGCAAATGGAAGGATTTGAACCCCAAGTTTGTACTCCAGGTCTACAGAGATTTCGCAGCAACAGGAGACATGGCATTTGGAATTGATGTTTGGCCTTCTGTTCGTGCTGCCATGGAGTATATGGACCAGTTTGACATGGATGGTGATGGCCTTATAGAGAATGATGGATTTCCAGATCAGACATATGATACATGGACAGTTCATGGAGTCAGTGCTTACTGTGGCTGTCTATGGCTTGCAGCACTTCAGGCTGCAGCAGCAATGGCTTTCCAATTAGGTGACGAGGAATTTGGTGAAAAATACAAGAGAAAATTTCAGATGGCCAAACATGCGTTTGAAGCCAAGCTGTGGAATGGCTCTTACTTCAATTATGACTCTGGATCAAGTAGTAACAGTAAATCTATTCAAGCTGATCAATTAGCTGGACAGTGGTACACAGCTTCGTCTGGGTTGCCTAATCTCTTCGATGACTTCAAAATCAAGAGCACCCTGCAGAAATTCTTTGATTACAATGTCATGAAAGTTAAAGGAGGCAGGGCAGGTGCTGTAAATGGGATACATCCCAACGGAAAGGTGGATGAGACCTGCATGCAATCACGTGAAATTTGGACTGGAGTTACTTATGCTGTGGCTGCTAAAATGATTCTTGCAGGGATGGAGGAGCAGGCCTTCTCAACTGCTGAAGGAATTTTCATTGCTGGATGGTCTGAAGACGGATATGGATATTGGTTTCAAACCCCGGAGGCTTGGACAATGGATGGCCATTTCCGTTCTCTAATTTACATGAGGCCGTTGGCAATTTGGGAAATGCAGTGGGCATTATCCATGCCTACAGCAGTTATTGAGGCGCCTAAGATTAACATAATGGACAGGTCTGATTTGCCTGCTTTAAGTTCAAGATCCACTCACAACGAGGCAGGTGGAAGAAAGGCCTCAAGAAAAATGAAGTGTTTCGGGAACTCAGTGTTTAGTTGGGCATGTTAA

Protein sequence

MWCTYDVHFYASFALLDLFPKIELSIQREFAKAVLSEDGRKVRFLAEGNWGIRKVRGAVPHDLGTHDPWKEMNAYNIHDTSKWKDLNPKFVLQVYRDFAATGDMAFGIDVWPSVRAAMEYMDQFDMDGDGLIENDGFPDQTYDTWTVHGVSAYCGCLWLAALQAAAAMAFQLGDEEFGEKYKRKFQMAKHAFEAKLWNGSYFNYDSGSSSNSKSIQADQLAGQWYTASSGLPNLFDDFKIKSTLQKFFDYNVMKVKGGRAGAVNGIHPNGKVDETCMQSREIWTGVTYAVAAKMILAGMEEQAFSTAEGIFIAGWSEDGYGYWFQTPEAWTMDGHFRSLIYMRPLAIWEMQWALSMPTAVIEAPKINIMDRSDLPALSSRSTHNEAGGRKASRKMKCFGNSVFSWAC

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo15068	Spo15068	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo15068.1	Spo15068.1-protein	polypeptide

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo15068.1.exon.1	Spo15068.1.exon.1	exon
Spo15068.1.exon.2	Spo15068.1.exon.2	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo15068.1.CDS.1	Spo15068.1.CDS.1	CDS
Spo15068.1.CDS.2	Spo15068.1.CDS.2	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo15068.1.utr3p.1	Spo15068.1.utr3p.1	three_prime_UTR

Homology

[Close]

BLAST of Spo15068.1 vs. NCBI nr
Match: gi|902195156|gb|KNA12766.1| (hypothetical protein SOVF_122950 isoform B [Spinacia oleracea])

HSP 1 Score: 846.3 bits (2185), Expect = 2.300e-242
Identity = 405/407 (99.51%), Postives = 405/407 (99.51%), Query Frame = 1


		  
Query: 1   MWCTYDVHFYASFALLDLFPKIELSIQREFAKAVLSEDGRKVRFLAEGNWGIRKVRGAVP 60
           MWCTYDVHFYASFALLDLFPKIELSIQREFAKAVLSEDGRKVRFLAEGNWGIRKVRGAVP
Sbjct: 138 MWCTYDVHFYASFALLDLFPKIELSIQREFAKAVLSEDGRKVRFLAEGNWGIRKVRGAVP 197

Query: 61  HDLGTHDPWKEMNAYNIHDTSKWKDLNPKFVLQVYRDFAATGDMAFGIDVWPSVRAAMEY 120
           HDLGTHDPWKEMNAYNIHDTSKWKDLNPKFVLQVYRDFAATGDMAFGIDVWPSVRAAMEY
Sbjct: 198 HDLGTHDPWKEMNAYNIHDTSKWKDLNPKFVLQVYRDFAATGDMAFGIDVWPSVRAAMEY 257

Query: 121 MDQFDMDGDGLIENDGFPDQTYDTWTVHGVSAYCGCLWLAALQAAAAMAFQLGDEEFGEK 180
           MDQFDMDGDGLIENDGFPDQTYDTWTVHGVSAYCGCLWLAALQAAAAMAFQLG EEFGEK
Sbjct: 258 MDQFDMDGDGLIENDGFPDQTYDTWTVHGVSAYCGCLWLAALQAAAAMAFQLGHEEFGEK 317

Query: 181 YKRKFQMAKHAFEAKLWNGSYFNYDSGSSSNSKSIQADQLAGQWYTASSGLPNLFDDFKI 240
           YKRKFQMAKHAFEAKLWNGSYFNYDSGSSSNSKSIQADQLAGQWYTASSGLPNLFDDFKI
Sbjct: 318 YKRKFQMAKHAFEAKLWNGSYFNYDSGSSSNSKSIQADQLAGQWYTASSGLPNLFDDFKI 377

Query: 241 KSTLQKFFDYNVMKVKGGRAGAVNGIHPNGKVDETCMQSREIWTGVTYAVAAKMILAGME 300
           KSTLQK FDYNVMKVKGGRAGAVNGIHPNGKVDETCMQSREIWTGVTYAVAAKMILAGME
Sbjct: 378 KSTLQKIFDYNVMKVKGGRAGAVNGIHPNGKVDETCMQSREIWTGVTYAVAAKMILAGME 437

Query: 301 EQAFSTAEGIFIAGWSEDGYGYWFQTPEAWTMDGHFRSLIYMRPLAIWEMQWALSMPTAV 360
           EQAFSTAEGIFIAGWSEDGYGYWFQTPEAWTMDGHFRSLIYMRPLAIWEMQWALSMPTAV
Sbjct: 438 EQAFSTAEGIFIAGWSEDGYGYWFQTPEAWTMDGHFRSLIYMRPLAIWEMQWALSMPTAV 497

Query: 361 IEAPKINIMDRSDLPALSSRSTHNEAGGRKASRKMKCFGNSVFSWAC 408
           IEAPKINIMDRSDLPALSSRSTHNEAGGRKASRKMKCFGNSVFSWAC
Sbjct: 498 IEAPKINIMDRSDLPALSSRSTHNEAGGRKASRKMKCFGNSVFSWAC 544

[Close]

BLAST of Spo15068.1 vs. NCBI nr
Match: gi|902195155|gb|KNA12765.1| (hypothetical protein SOVF_122950 isoform A [Spinacia oleracea])

HSP 1 Score: 846.3 bits (2185), Expect = 2.300e-242
Identity = 405/407 (99.51%), Postives = 405/407 (99.51%), Query Frame = 1


		  
Query: 1   MWCTYDVHFYASFALLDLFPKIELSIQREFAKAVLSEDGRKVRFLAEGNWGIRKVRGAVP 60
           MWCTYDVHFYASFALLDLFPKIELSIQREFAKAVLSEDGRKVRFLAEGNWGIRKVRGAVP
Sbjct: 539 MWCTYDVHFYASFALLDLFPKIELSIQREFAKAVLSEDGRKVRFLAEGNWGIRKVRGAVP 598

Query: 61  HDLGTHDPWKEMNAYNIHDTSKWKDLNPKFVLQVYRDFAATGDMAFGIDVWPSVRAAMEY 120
           HDLGTHDPWKEMNAYNIHDTSKWKDLNPKFVLQVYRDFAATGDMAFGIDVWPSVRAAMEY
Sbjct: 599 HDLGTHDPWKEMNAYNIHDTSKWKDLNPKFVLQVYRDFAATGDMAFGIDVWPSVRAAMEY 658

Query: 121 MDQFDMDGDGLIENDGFPDQTYDTWTVHGVSAYCGCLWLAALQAAAAMAFQLGDEEFGEK 180
           MDQFDMDGDGLIENDGFPDQTYDTWTVHGVSAYCGCLWLAALQAAAAMAFQLG EEFGEK
Sbjct: 659 MDQFDMDGDGLIENDGFPDQTYDTWTVHGVSAYCGCLWLAALQAAAAMAFQLGHEEFGEK 718

Query: 181 YKRKFQMAKHAFEAKLWNGSYFNYDSGSSSNSKSIQADQLAGQWYTASSGLPNLFDDFKI 240
           YKRKFQMAKHAFEAKLWNGSYFNYDSGSSSNSKSIQADQLAGQWYTASSGLPNLFDDFKI
Sbjct: 719 YKRKFQMAKHAFEAKLWNGSYFNYDSGSSSNSKSIQADQLAGQWYTASSGLPNLFDDFKI 778

Query: 241 KSTLQKFFDYNVMKVKGGRAGAVNGIHPNGKVDETCMQSREIWTGVTYAVAAKMILAGME 300
           KSTLQK FDYNVMKVKGGRAGAVNGIHPNGKVDETCMQSREIWTGVTYAVAAKMILAGME
Sbjct: 779 KSTLQKIFDYNVMKVKGGRAGAVNGIHPNGKVDETCMQSREIWTGVTYAVAAKMILAGME 838

Query: 301 EQAFSTAEGIFIAGWSEDGYGYWFQTPEAWTMDGHFRSLIYMRPLAIWEMQWALSMPTAV 360
           EQAFSTAEGIFIAGWSEDGYGYWFQTPEAWTMDGHFRSLIYMRPLAIWEMQWALSMPTAV
Sbjct: 839 EQAFSTAEGIFIAGWSEDGYGYWFQTPEAWTMDGHFRSLIYMRPLAIWEMQWALSMPTAV 898

Query: 361 IEAPKINIMDRSDLPALSSRSTHNEAGGRKASRKMKCFGNSVFSWAC 408
           IEAPKINIMDRSDLPALSSRSTHNEAGGRKASRKMKCFGNSVFSWAC
Sbjct: 899 IEAPKINIMDRSDLPALSSRSTHNEAGGRKASRKMKCFGNSVFSWAC 945

[Close]

BLAST of Spo15068.1 vs. NCBI nr
Match: gi|731320443|ref|XP_010670809.1| (PREDICTED: non-lysosomal glucosylceramidase [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 731.9 bits (1888), Expect = 6.200e-208
Identity = 356/412 (86.41%), Postives = 376/412 (91.26%), Query Frame = 1


		  
Query: 1   MWCTYDVHFYASFALLDLFPKIELSIQREFAKAVLSEDGRKVRFLAEGNWGIRKVRGAVP 60
           MWCTYDVHFYASFALLDLFPKIELSIQREFAKAVLSEDGR+VRFLAEGN GIRKVRGAVP
Sbjct: 562 MWCTYDVHFYASFALLDLFPKIELSIQREFAKAVLSEDGRRVRFLAEGNVGIRKVRGAVP 621

Query: 61  HDLGTHDPWKEMNAYNIHDTSKWKDLNPKFVLQVYRDFAATGDMAFGIDVWPSVRAAMEY 120
           HDLGTHDPWKEMNAYNIHDTSKWKDLN KFVLQVYRDF+ATGDM+FG DVWPSVRAA+EY
Sbjct: 622 HDLGTHDPWKEMNAYNIHDTSKWKDLNTKFVLQVYRDFSATGDMSFGADVWPSVRAAIEY 681

Query: 121 MDQFDMDGDGLIENDGFPDQTYDTWTVHGVSAYCGCLWLAALQAAAAMAFQLGDEEFGEK 180
           M QFD D DGLIENDGFPDQTYDTWTVHGVSAYCG LWLAALQAAAAMA QLGDEEFGEK
Sbjct: 682 MYQFDTDDDGLIENDGFPDQTYDTWTVHGVSAYCGGLWLAALQAAAAMASQLGDEEFGEK 741

Query: 181 YKRKFQMAKHAFEAKLWNGSYFNYDSGSSSNSKSIQADQLAGQWYTASSGLPNLFDDFKI 240
            K+K  +AK+AFE KLWNGSYFNYDSGSSSNSKSIQADQLAGQWYTASSGLPNLFDD +I
Sbjct: 742 CKKKLLVAKNAFETKLWNGSYFNYDSGSSSNSKSIQADQLAGQWYTASSGLPNLFDDNQI 801

Query: 241 KSTLQKFFDYNVMKVKGGRAGAVNGIHPNGKVDETCMQSREIWTGVTYAVAAKMILAGME 300
           KSTLQK FDYNVMKVKGGR GAVNG+HP+GKVDETCMQSREIW+GVTYAVAA MIL+GME
Sbjct: 802 KSTLQKIFDYNVMKVKGGRMGAVNGMHPSGKVDETCMQSREIWSGVTYAVAATMILSGME 861

Query: 301 EQAFSTAEGIFIAGWSEDGYGYWFQTPEAWTMDGHFRSLIYMRPLAIWEMQWALSMPTAV 360
           EQ FSTAEGIFIAGWSEDG+GYWFQTPEAWT+DGH+RSLIYMRPLAIW MQWALSMP AV
Sbjct: 862 EQGFSTAEGIFIAGWSEDGFGYWFQTPEAWTLDGHYRSLIYMRPLAIWGMQWALSMPRAV 921

Query: 361 IEAPKINIMDRSDLPALSSRST------HNEAGGRKASRKMKCFGNSVFSWA 407
           ++ P+INIMDR DL  L SRST        EA  RKAS K+KCFGNS+FS A
Sbjct: 922 LQPPRINIMDRIDLSDLDSRSTLSLTSARTEAARRKASGKLKCFGNSMFSCA 973

[Close]

BLAST of Spo15068.1 vs. NCBI nr
Match: gi|1012241463|ref|XP_015941005.1| (PREDICTED: non-lysosomal glucosylceramidase [Arachis duranensis])

HSP 1 Score: 720.7 bits (1859), Expect = 1.400e-204
Identity = 340/407 (83.54%), Postives = 370/407 (90.91%), Query Frame = 1


		  
Query: 1   MWCTYDVHFYASFALLDLFPKIELSIQREFAKAVLSEDGRKVRFLAEGNWGIRKVRGAVP 60
           MWCTYDVHFYASFALL+LFP+IEL+IQR+FAKAVL EDGRKV+FLAEGN GIRKVRGAVP
Sbjct: 574 MWCTYDVHFYASFALLELFPRIELNIQRDFAKAVLCEDGRKVKFLAEGNCGIRKVRGAVP 633

Query: 61  HDLGTHDPWKEMNAYNIHDTSKWKDLNPKFVLQVYRDFAATGDMAFGIDVWPSVRAAMEY 120
           HDLGTHDPW+EMNAYNIHDTSKWKDLNPKFVLQVYRDFAATGDM+FG+DVWP+VRAAMEY
Sbjct: 634 HDLGTHDPWREMNAYNIHDTSKWKDLNPKFVLQVYRDFAATGDMSFGVDVWPAVRAAMEY 693

Query: 121 MDQFDMDGDGLIENDGFPDQTYDTWTVHGVSAYCGCLWLAALQAAAAMAFQLGDEEFGEK 180
           M+QFD DGDGLIENDGFPDQTYDTWTVHGVSAYCGCLWLAALQAAAAMA QLGD +F E 
Sbjct: 694 MEQFDRDGDGLIENDGFPDQTYDTWTVHGVSAYCGCLWLAALQAAAAMALQLGDRDFAET 753

Query: 181 YKRKFQMAKHAFEAKLWNGSYFNYDSGSSSNSKSIQADQLAGQWYTASSGLPNLFDDFKI 240
            KRKF  AK AFE KLWNGSYFNYDSGSSSNSKSIQADQLAGQWYTASSGLP LFD+ KI
Sbjct: 754 CKRKFLKAKPAFEQKLWNGSYFNYDSGSSSNSKSIQADQLAGQWYTASSGLPPLFDESKI 813

Query: 241 KSTLQKFFDYNVMKVKGGRAGAVNGIHPNGKVDETCMQSREIWTGVTYAVAAKMILAGME 300
           KS L+K +D+NVMKVKGG+ GAVNG+HPNGKVDETCMQSREIWTGVTY VA+ MILAGME
Sbjct: 814 KSALRKVYDFNVMKVKGGKMGAVNGMHPNGKVDETCMQSREIWTGVTYGVASTMILAGME 873

Query: 301 EQAFSTAEGIFIAGWSEDGYGYWFQTPEAWTMDGHFRSLIYMRPLAIWEMQWALSMPTAV 360
           E+AF+TAEGIF AGWSEDGYGYWFQTPEAWTMDGH+RSLIYMRPL+IW MQ+AL++P A+
Sbjct: 874 EEAFATAEGIFQAGWSEDGYGYWFQTPEAWTMDGHYRSLIYMRPLSIWGMQYALTLPKAI 933

Query: 361 IEAPKINIMDRSDLPALSSRSTHNEAGGRKASRKMKCFGNSVFSWAC 408
           ++APKINIMDR  L  L+    HNE G RK + K KCFGNSVF  AC
Sbjct: 934 LDAPKINIMDRIHLSPLNGGFPHNETGVRKIANKAKCFGNSVFHCAC 980

[Close]

BLAST of Spo15068.1 vs. NCBI nr
Match: gi|1021567737|ref|XP_016175090.1| (PREDICTED: non-lysosomal glucosylceramidase [Arachis ipaensis])

HSP 1 Score: 720.7 bits (1859), Expect = 1.400e-204
Identity = 340/407 (83.54%), Postives = 370/407 (90.91%), Query Frame = 1


		  
Query: 1   MWCTYDVHFYASFALLDLFPKIELSIQREFAKAVLSEDGRKVRFLAEGNWGIRKVRGAVP 60
           MWCTYDVHFYASFALL+LFP+IEL+IQR+FAKAVL EDGRKV+FLAEGN GIRKVRGAVP
Sbjct: 574 MWCTYDVHFYASFALLELFPRIELNIQRDFAKAVLCEDGRKVKFLAEGNCGIRKVRGAVP 633

Query: 61  HDLGTHDPWKEMNAYNIHDTSKWKDLNPKFVLQVYRDFAATGDMAFGIDVWPSVRAAMEY 120
           HDLGTHDPW+EMNAYNIHDTSKWKDLNPKFVLQVYRDFAATGDM+FG+DVWP+VRAAMEY
Sbjct: 634 HDLGTHDPWREMNAYNIHDTSKWKDLNPKFVLQVYRDFAATGDMSFGVDVWPAVRAAMEY 693

Query: 121 MDQFDMDGDGLIENDGFPDQTYDTWTVHGVSAYCGCLWLAALQAAAAMAFQLGDEEFGEK 180
           M+QFD DGDGLIENDGFPDQTYDTWTVHGVSAYCGCLWLAALQAAAAMA QLGD +F E 
Sbjct: 694 MEQFDRDGDGLIENDGFPDQTYDTWTVHGVSAYCGCLWLAALQAAAAMALQLGDRDFAET 753

Query: 181 YKRKFQMAKHAFEAKLWNGSYFNYDSGSSSNSKSIQADQLAGQWYTASSGLPNLFDDFKI 240
            KRKF  AK AFE KLWNGSYFNYDSGSSSNSKSIQADQLAGQWYTASSGLP LFD+ KI
Sbjct: 754 CKRKFLKAKPAFEQKLWNGSYFNYDSGSSSNSKSIQADQLAGQWYTASSGLPPLFDESKI 813

Query: 241 KSTLQKFFDYNVMKVKGGRAGAVNGIHPNGKVDETCMQSREIWTGVTYAVAAKMILAGME 300
           KS L+K +D+NVMKVKGG+ GAVNG+HPNGKVDETCMQSREIWTGVTY VA+ MILAGME
Sbjct: 814 KSALRKVYDFNVMKVKGGKMGAVNGMHPNGKVDETCMQSREIWTGVTYGVASTMILAGME 873

Query: 301 EQAFSTAEGIFIAGWSEDGYGYWFQTPEAWTMDGHFRSLIYMRPLAIWEMQWALSMPTAV 360
           E+AF+TAEGIF AGWSEDGYGYWFQTPEAWTMDGH+RSLIYMRPL+IW MQ+AL++P A+
Sbjct: 874 EEAFATAEGIFQAGWSEDGYGYWFQTPEAWTMDGHYRSLIYMRPLSIWGMQYALTLPKAI 933

Query: 361 IEAPKINIMDRSDLPALSSRSTHNEAGGRKASRKMKCFGNSVFSWAC 408
           ++APKINIMDR  L  L+    HNE G RK + K KCFGNSVF  AC
Sbjct: 934 LDAPKINIMDRIHLSPLNGGFPHNETGVRKIANKAKCFGNSVFHCAC 980

[Close]

BLAST of Spo15068.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QZS3_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_122950 PE=4 SV=1)

HSP 1 Score: 846.3 bits (2185), Expect = 1.600e-242
Identity = 405/407 (99.51%), Postives = 405/407 (99.51%), Query Frame = 1


		  
Query: 1   MWCTYDVHFYASFALLDLFPKIELSIQREFAKAVLSEDGRKVRFLAEGNWGIRKVRGAVP 60
           MWCTYDVHFYASFALLDLFPKIELSIQREFAKAVLSEDGRKVRFLAEGNWGIRKVRGAVP
Sbjct: 539 MWCTYDVHFYASFALLDLFPKIELSIQREFAKAVLSEDGRKVRFLAEGNWGIRKVRGAVP 598

Query: 61  HDLGTHDPWKEMNAYNIHDTSKWKDLNPKFVLQVYRDFAATGDMAFGIDVWPSVRAAMEY 120
           HDLGTHDPWKEMNAYNIHDTSKWKDLNPKFVLQVYRDFAATGDMAFGIDVWPSVRAAMEY
Sbjct: 599 HDLGTHDPWKEMNAYNIHDTSKWKDLNPKFVLQVYRDFAATGDMAFGIDVWPSVRAAMEY 658

Query: 121 MDQFDMDGDGLIENDGFPDQTYDTWTVHGVSAYCGCLWLAALQAAAAMAFQLGDEEFGEK 180
           MDQFDMDGDGLIENDGFPDQTYDTWTVHGVSAYCGCLWLAALQAAAAMAFQLG EEFGEK
Sbjct: 659 MDQFDMDGDGLIENDGFPDQTYDTWTVHGVSAYCGCLWLAALQAAAAMAFQLGHEEFGEK 718

Query: 181 YKRKFQMAKHAFEAKLWNGSYFNYDSGSSSNSKSIQADQLAGQWYTASSGLPNLFDDFKI 240
           YKRKFQMAKHAFEAKLWNGSYFNYDSGSSSNSKSIQADQLAGQWYTASSGLPNLFDDFKI
Sbjct: 719 YKRKFQMAKHAFEAKLWNGSYFNYDSGSSSNSKSIQADQLAGQWYTASSGLPNLFDDFKI 778

Query: 241 KSTLQKFFDYNVMKVKGGRAGAVNGIHPNGKVDETCMQSREIWTGVTYAVAAKMILAGME 300
           KSTLQK FDYNVMKVKGGRAGAVNGIHPNGKVDETCMQSREIWTGVTYAVAAKMILAGME
Sbjct: 779 KSTLQKIFDYNVMKVKGGRAGAVNGIHPNGKVDETCMQSREIWTGVTYAVAAKMILAGME 838

Query: 301 EQAFSTAEGIFIAGWSEDGYGYWFQTPEAWTMDGHFRSLIYMRPLAIWEMQWALSMPTAV 360
           EQAFSTAEGIFIAGWSEDGYGYWFQTPEAWTMDGHFRSLIYMRPLAIWEMQWALSMPTAV
Sbjct: 839 EQAFSTAEGIFIAGWSEDGYGYWFQTPEAWTMDGHFRSLIYMRPLAIWEMQWALSMPTAV 898

Query: 361 IEAPKINIMDRSDLPALSSRSTHNEAGGRKASRKMKCFGNSVFSWAC 408
           IEAPKINIMDRSDLPALSSRSTHNEAGGRKASRKMKCFGNSVFSWAC
Sbjct: 899 IEAPKINIMDRSDLPALSSRSTHNEAGGRKASRKMKCFGNSVFSWAC 945

[Close]

BLAST of Spo15068.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QZS9_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_122950 PE=4 SV=1)

HSP 1 Score: 846.3 bits (2185), Expect = 1.600e-242
Identity = 405/407 (99.51%), Postives = 405/407 (99.51%), Query Frame = 1


		  
Query: 1   MWCTYDVHFYASFALLDLFPKIELSIQREFAKAVLSEDGRKVRFLAEGNWGIRKVRGAVP 60
           MWCTYDVHFYASFALLDLFPKIELSIQREFAKAVLSEDGRKVRFLAEGNWGIRKVRGAVP
Sbjct: 138 MWCTYDVHFYASFALLDLFPKIELSIQREFAKAVLSEDGRKVRFLAEGNWGIRKVRGAVP 197

Query: 61  HDLGTHDPWKEMNAYNIHDTSKWKDLNPKFVLQVYRDFAATGDMAFGIDVWPSVRAAMEY 120
           HDLGTHDPWKEMNAYNIHDTSKWKDLNPKFVLQVYRDFAATGDMAFGIDVWPSVRAAMEY
Sbjct: 198 HDLGTHDPWKEMNAYNIHDTSKWKDLNPKFVLQVYRDFAATGDMAFGIDVWPSVRAAMEY 257

Query: 121 MDQFDMDGDGLIENDGFPDQTYDTWTVHGVSAYCGCLWLAALQAAAAMAFQLGDEEFGEK 180
           MDQFDMDGDGLIENDGFPDQTYDTWTVHGVSAYCGCLWLAALQAAAAMAFQLG EEFGEK
Sbjct: 258 MDQFDMDGDGLIENDGFPDQTYDTWTVHGVSAYCGCLWLAALQAAAAMAFQLGHEEFGEK 317

Query: 181 YKRKFQMAKHAFEAKLWNGSYFNYDSGSSSNSKSIQADQLAGQWYTASSGLPNLFDDFKI 240
           YKRKFQMAKHAFEAKLWNGSYFNYDSGSSSNSKSIQADQLAGQWYTASSGLPNLFDDFKI
Sbjct: 318 YKRKFQMAKHAFEAKLWNGSYFNYDSGSSSNSKSIQADQLAGQWYTASSGLPNLFDDFKI 377

Query: 241 KSTLQKFFDYNVMKVKGGRAGAVNGIHPNGKVDETCMQSREIWTGVTYAVAAKMILAGME 300
           KSTLQK FDYNVMKVKGGRAGAVNGIHPNGKVDETCMQSREIWTGVTYAVAAKMILAGME
Sbjct: 378 KSTLQKIFDYNVMKVKGGRAGAVNGIHPNGKVDETCMQSREIWTGVTYAVAAKMILAGME 437

Query: 301 EQAFSTAEGIFIAGWSEDGYGYWFQTPEAWTMDGHFRSLIYMRPLAIWEMQWALSMPTAV 360
           EQAFSTAEGIFIAGWSEDGYGYWFQTPEAWTMDGHFRSLIYMRPLAIWEMQWALSMPTAV
Sbjct: 438 EQAFSTAEGIFIAGWSEDGYGYWFQTPEAWTMDGHFRSLIYMRPLAIWEMQWALSMPTAV 497

Query: 361 IEAPKINIMDRSDLPALSSRSTHNEAGGRKASRKMKCFGNSVFSWAC 408
           IEAPKINIMDRSDLPALSSRSTHNEAGGRKASRKMKCFGNSVFSWAC
Sbjct: 498 IEAPKINIMDRSDLPALSSRSTHNEAGGRKASRKMKCFGNSVFSWAC 544

[Close]

BLAST of Spo15068.1 vs. UniProtKB/TrEMBL
Match: A0A0J8CT40_BETVU (Non-lysosomal glucosylceramidase OS=Beta vulgaris subsp. vulgaris GN=BVRB_2g044000 PE=3 SV=1)

HSP 1 Score: 731.9 bits (1888), Expect = 4.300e-208
Identity = 356/412 (86.41%), Postives = 376/412 (91.26%), Query Frame = 1


		  
Query: 1   MWCTYDVHFYASFALLDLFPKIELSIQREFAKAVLSEDGRKVRFLAEGNWGIRKVRGAVP 60
           MWCTYDVHFYASFALLDLFPKIELSIQREFAKAVLSEDGR+VRFLAEGN GIRKVRGAVP
Sbjct: 562 MWCTYDVHFYASFALLDLFPKIELSIQREFAKAVLSEDGRRVRFLAEGNVGIRKVRGAVP 621

Query: 61  HDLGTHDPWKEMNAYNIHDTSKWKDLNPKFVLQVYRDFAATGDMAFGIDVWPSVRAAMEY 120
           HDLGTHDPWKEMNAYNIHDTSKWKDLN KFVLQVYRDF+ATGDM+FG DVWPSVRAA+EY
Sbjct: 622 HDLGTHDPWKEMNAYNIHDTSKWKDLNTKFVLQVYRDFSATGDMSFGADVWPSVRAAIEY 681

Query: 121 MDQFDMDGDGLIENDGFPDQTYDTWTVHGVSAYCGCLWLAALQAAAAMAFQLGDEEFGEK 180
           M QFD D DGLIENDGFPDQTYDTWTVHGVSAYCG LWLAALQAAAAMA QLGDEEFGEK
Sbjct: 682 MYQFDTDDDGLIENDGFPDQTYDTWTVHGVSAYCGGLWLAALQAAAAMASQLGDEEFGEK 741

Query: 181 YKRKFQMAKHAFEAKLWNGSYFNYDSGSSSNSKSIQADQLAGQWYTASSGLPNLFDDFKI 240
            K+K  +AK+AFE KLWNGSYFNYDSGSSSNSKSIQADQLAGQWYTASSGLPNLFDD +I
Sbjct: 742 CKKKLLVAKNAFETKLWNGSYFNYDSGSSSNSKSIQADQLAGQWYTASSGLPNLFDDNQI 801

Query: 241 KSTLQKFFDYNVMKVKGGRAGAVNGIHPNGKVDETCMQSREIWTGVTYAVAAKMILAGME 300
           KSTLQK FDYNVMKVKGGR GAVNG+HP+GKVDETCMQSREIW+GVTYAVAA MIL+GME
Sbjct: 802 KSTLQKIFDYNVMKVKGGRMGAVNGMHPSGKVDETCMQSREIWSGVTYAVAATMILSGME 861

Query: 301 EQAFSTAEGIFIAGWSEDGYGYWFQTPEAWTMDGHFRSLIYMRPLAIWEMQWALSMPTAV 360
           EQ FSTAEGIFIAGWSEDG+GYWFQTPEAWT+DGH+RSLIYMRPLAIW MQWALSMP AV
Sbjct: 862 EQGFSTAEGIFIAGWSEDGFGYWFQTPEAWTLDGHYRSLIYMRPLAIWGMQWALSMPRAV 921

Query: 361 IEAPKINIMDRSDLPALSSRST------HNEAGGRKASRKMKCFGNSVFSWA 407
           ++ P+INIMDR DL  L SRST        EA  RKAS K+KCFGNS+FS A
Sbjct: 922 LQPPRINIMDRIDLSDLDSRSTLSLTSARTEAARRKASGKLKCFGNSMFSCA 973

[Close]

BLAST of Spo15068.1 vs. UniProtKB/TrEMBL
Match: M5Y423_PRUPE (Non-lysosomal glucosylceramidase OS=Prunus persica GN=PRUPE_ppa001012mg PE=3 SV=1)

HSP 1 Score: 719.9 bits (1857), Expect = 1.700e-204
Identity = 340/407 (83.54%), Postives = 372/407 (91.40%), Query Frame = 1


		  
Query: 1   MWCTYDVHFYASFALLDLFPKIELSIQREFAKAVLSEDGRKVRFLAEGNWGIRKVRGAVP 60
           MW TYDVHFYASFALL+LFPKIEL+IQR+FAKAVLSEDGRKV+FLAEGNWGIRKVRGAVP
Sbjct: 528 MWNTYDVHFYASFALLELFPKIELNIQRDFAKAVLSEDGRKVKFLAEGNWGIRKVRGAVP 587

Query: 61  HDLGTHDPWKEMNAYNIHDTSKWKDLNPKFVLQVYRDFAATGDMAFGIDVWPSVRAAMEY 120
           HDLGTHDPW EMNAYNIHDTSKWKDLNPKFVLQVYRDF+ATGDMAFG+DVWP+VRAAMEY
Sbjct: 588 HDLGTHDPWNEMNAYNIHDTSKWKDLNPKFVLQVYRDFSATGDMAFGVDVWPAVRAAMEY 647

Query: 121 MDQFDMDGDGLIENDGFPDQTYDTWTVHGVSAYCGCLWLAALQAAAAMAFQLGDEEFGEK 180
           M+QFD D DGLIENDGFPDQTYD WTVHGVSAYCGCLWLAALQAAAAMAFQLGD+ F E 
Sbjct: 648 MEQFDRDNDGLIENDGFPDQTYDAWTVHGVSAYCGCLWLAALQAAAAMAFQLGDKAFAEW 707

Query: 181 YKRKFQMAKHAFEAKLWNGSYFNYDSGSSSNSKSIQADQLAGQWYTASSGLPNLFDDFKI 240
            K K+  AK AFE KLWNGSYFNYDSGSSSNSKSIQADQLAGQWYTASSGLP+LFDDFKI
Sbjct: 708 CKTKYLKAKPAFEEKLWNGSYFNYDSGSSSNSKSIQADQLAGQWYTASSGLPSLFDDFKI 767

Query: 241 KSTLQKFFDYNVMKVKGGRAGAVNGIHPNGKVDETCMQSREIWTGVTYAVAAKMILAGME 300
           +S LQK +D+NVMKVKGG+ GAVNG+HP+GKVDE+CMQSREIWTGVTY VAA MILAG E
Sbjct: 768 QSALQKIYDFNVMKVKGGQMGAVNGMHPSGKVDESCMQSREIWTGVTYGVAATMILAGKE 827

Query: 301 EQAFSTAEGIFIAGWSEDGYGYWFQTPEAWTMDGHFRSLIYMRPLAIWEMQWALSMPTAV 360
           ++AF+TAEGIFIAGWSE+GYGY FQTPE WTMDGHFRSLIYMRPL+IW MQWAL++P A+
Sbjct: 828 KEAFTTAEGIFIAGWSEEGYGYGFQTPEGWTMDGHFRSLIYMRPLSIWAMQWALNLPKAI 887

Query: 361 IEAPKINIMDRSDLPALSSRSTHNEAGGRKASRKMKCFGNSVFSWAC 408
           +EAP INIMDR  L + SSRS+ NE+G RK + K KCFGNSVF+ AC
Sbjct: 888 LEAPTINIMDRIHLSSFSSRSSQNESGVRKIATKAKCFGNSVFNCAC 934

[Close]

BLAST of Spo15068.1 vs. UniProtKB/TrEMBL
Match: A0A0S3SPA3_PHAAN (Non-lysosomal glucosylceramidase OS=Vigna angularis var. angularis GN=Vigan.08G128200 PE=3 SV=1)

HSP 1 Score: 716.1 bits (1847), Expect = 2.500e-203
Identity = 336/407 (82.56%), Postives = 366/407 (89.93%), Query Frame = 1


		  
Query: 1   MWCTYDVHFYASFALLDLFPKIELSIQREFAKAVLSEDGRKVRFLAEGNWGIRKVRGAVP 60
           MWCTYDVHFYASFALL+LFP+IEL+IQR+FA+AVL EDGRKVRFLAEGNWGIRKV GAVP
Sbjct: 548 MWCTYDVHFYASFALLELFPRIELNIQRDFARAVLCEDGRKVRFLAEGNWGIRKVYGAVP 607

Query: 61  HDLGTHDPWKEMNAYNIHDTSKWKDLNPKFVLQVYRDFAATGDMAFGIDVWPSVRAAMEY 120
           HDLGTHDPW EMNAYNIHDTSKWKDLNPKFVLQVYRDFAATGD+ FGIDVWP+VRAAMEY
Sbjct: 608 HDLGTHDPWYEMNAYNIHDTSKWKDLNPKFVLQVYRDFAATGDLQFGIDVWPAVRAAMEY 667

Query: 121 MDQFDMDGDGLIENDGFPDQTYDTWTVHGVSAYCGCLWLAALQAAAAMAFQLGDEEFGEK 180
           MDQFD DGDGLIENDGFPDQTYDTWTVHGVS YCGCLWLAALQAAAAMA +LGD +F E 
Sbjct: 668 MDQFDRDGDGLIENDGFPDQTYDTWTVHGVSTYCGCLWLAALQAAAAMALELGDRDFAET 727

Query: 181 YKRKFQMAKHAFEAKLWNGSYFNYDSGSSSNSKSIQADQLAGQWYTASSGLPNLFDDFKI 240
            KRKF  AK AFE KLWNGSYFNYDSGSS NSKSIQADQLAGQWYTASSGLP+LF+D KI
Sbjct: 728 CKRKFLKAKPAFEEKLWNGSYFNYDSGSSGNSKSIQADQLAGQWYTASSGLPSLFEDSKI 787

Query: 241 KSTLQKFFDYNVMKVKGGRAGAVNGIHPNGKVDETCMQSREIWTGVTYAVAAKMILAGME 300
           KS L+K +D+NVMKVKGGR GAVNG+HPNGKVDETCMQSRE+WTGVTY VAA MILAGME
Sbjct: 788 KSALRKVYDFNVMKVKGGRMGAVNGMHPNGKVDETCMQSREVWTGVTYGVAATMILAGME 847

Query: 301 EQAFSTAEGIFIAGWSEDGYGYWFQTPEAWTMDGHFRSLIYMRPLAIWEMQWALSMPTAV 360
           E+AF+TAEGIF+AGWSEDGYGYWFQTPEAWTMDGH+RSL+YMRPLAIW MQ+A++ P A+
Sbjct: 848 EEAFTTAEGIFLAGWSEDGYGYWFQTPEAWTMDGHYRSLMYMRPLAIWGMQYAMNRPKAI 907

Query: 361 IEAPKINIMDRSDLPALSSRSTHNEAGGRKASRKMKCFGNSVFSWAC 408
           +EAPKINIMDR  L  +    +HNE G RK + K +CF NSVF  AC
Sbjct: 908 LEAPKINIMDRIHLSPVIGGFSHNETGVRKIATKARCFSNSVFHCAC 954

[Close]

BLAST of Spo15068.1 vs. ExPASy Swiss-Prot
Match: GBA2_MOUSE (Non-lysosomal glucosylceramidase OS=Mus musculus GN=Gba2 PE=1 SV=2)

HSP 1 Score: 341.3 bits (874), Expect = 1.500e-92
Identity = 178/360 (49.44%), Postives = 222/360 (61.67%), Query Frame = 1


		  
Query: 1   MWCTYDVHFYASFALLDLFPKIELSIQREFAKAVLSEDGRKVRFLAEGNWGIRKVRGAVP 60
           M+ TYDVHFYASFAL+ L+PK+ELS+Q + A A L ED  + R+L  G     K R  +P
Sbjct: 524 MYNTYDVHFYASFALVMLWPKLELSLQYDMALATLKEDLTRRRYLMSGVVAPVKRRNVIP 583

Query: 61  HDLGTHD--PWKEMNAYNIHDTSKWKDLNPKFVLQVYRDFAATGDMAFGIDVWPSVRAAM 120
           HD+G  D  PW  +NAY IHDT+ WKDLN KFVLQ+YRD+  TGD  F  D+WP   A M
Sbjct: 584 HDIGDPDDEPWLRVNAYLIHDTADWKDLNLKFVLQIYRDYYLTGDQGFLEDMWPVCLAVM 643

Query: 121 EYMDQFDMDGDGLIENDGFPDQTYDTWTVHGVSAYCGCLWLAALQAAAAMAFQLGDEEFG 180
           E   +FD D DGLIEN G+ DQTYD W   G SAYCG LWLAA+     MA   G ++  
Sbjct: 644 ESEMKFDKDQDGLIENGGYADQTYDAWVTTGPSAYCGGLWLAAVAVMVQMAVLCGAQDVQ 703

Query: 181 EKYKRKFQMAKHAFEAKLWNGSYFNYDSGSSSNSKSIQADQLAGQWYTASSGL----PNL 240
           E++       + A+E  LWNG Y+NYDS S   S+SI +DQ AGQW+  + GL      +
Sbjct: 704 ERFASILCRGREAYERLLWNGRYYNYDSSSHPQSRSIMSDQCAGQWFLRACGLGEGDTEV 763

Query: 241 FDDFKIKSTLQKFFDYNVMKVKGGRAGAVNGIHPNGKVDETCMQSREIWTGVTYAVAAKM 300
           F    +   LQ  F+ NV    GG  GAVNG+HP+G  D + +QS E+W GV Y +AA M
Sbjct: 764 FPTLHVVRALQTIFELNVQAFAGGAMGAVNGMHPHGVPDRSSVQSDEVWVGVVYGLAATM 823

Query: 301 ILAGMEEQAFSTAEGIFIAGWSEDGYGYWFQTPEAWTMDGHFRSLIYMRPLAIWEMQWAL 355
           I  G+  + F TAEG +   W  +  G  FQTPEA+     FRSL YMRPL+IW MQ AL
Sbjct: 824 IQEGLTWEGFRTAEGCYRTVW--ERLGLAFQTPEAYCQQQVFRSLAYMRPLSIWAMQLAL 881

[Close]

BLAST of Spo15068.1 vs. ExPASy Swiss-Prot
Match: GBA2_HUMAN (Non-lysosomal glucosylceramidase OS=Homo sapiens GN=GBA2 PE=1 SV=2)

HSP 1 Score: 338.2 bits (866), Expect = 1.300e-91
Identity = 177/360 (49.17%), Postives = 222/360 (61.67%), Query Frame = 1


		  
Query: 1   MWCTYDVHFYASFALLDLFPKIELSIQREFAKAVLSEDGRKVRFLAEGNWGIRKVRGAVP 60
           M+ TYDVHFYASFAL+ L+PK+ELS+Q + A A L ED  + R+L  G     K R  +P
Sbjct: 533 MYNTYDVHFYASFALIMLWPKLELSLQYDMALATLREDLTRRRYLMSGVMAPVKRRNVIP 592

Query: 61  HDLGTHD--PWKEMNAYNIHDTSKWKDLNPKFVLQVYRDFAATGDMAFGIDVWPSVRAAM 120
           HD+G  D  PW  +NAY IHDT+ WKDLN KFVLQVYRD+  TGD  F  D+WP   A M
Sbjct: 593 HDIGDPDDEPWLRVNAYLIHDTADWKDLNLKFVLQVYRDYYLTGDQNFLKDMWPVCLAVM 652

Query: 121 EYMDQFDMDGDGLIENDGFPDQTYDTWTVHGVSAYCGCLWLAALQAAAAMAFQLGDEEFG 180
           E   +FD D DGLIEN G+ DQTYD W   G SAYCG LWLAA+     MA   G ++  
Sbjct: 653 ESEMKFDKDHDGLIENGGYADQTYDGWVTTGPSAYCGGLWLAAVAVMVQMAALCGAQDIQ 712

Query: 181 EKYKRKFQMAKHAFEAKLWNGSYFNYDSGSSSNSKSIQADQLAGQWYTASSGL----PNL 240
           +K+       + A+E  LWNG Y+NYDS S   S+S+ +DQ AGQW+  + GL      +
Sbjct: 713 DKFSSILSRGQEAYERLLWNGRYYNYDSSSRPQSRSVMSDQCAGQWFLKACGLGEGDTEV 772

Query: 241 FDDFKIKSTLQKFFDYNVMKVKGGRAGAVNGIHPNGKVDETCMQSREIWTGVTYAVAAKM 300
           F    +   LQ  F+ NV    GG  GAVNG+ P+G  D++ +QS E+W GV Y +AA M
Sbjct: 773 FPTQHVVRALQTIFELNVQAFAGGAMGAVNGMQPHGVPDKSSVQSDEVWVGVVYGLAATM 832

Query: 301 ILAGMEEQAFSTAEGIFIAGWSEDGYGYWFQTPEAWTMDGHFRSLIYMRPLAIWEMQWAL 355
           I  G+  + F TAEG +   W  +  G  FQTPEA+     FRSL YMRPL+IW MQ AL
Sbjct: 833 IQEGLTWEGFQTAEGCYRTVW--ERLGLAFQTPEAYCQQRVFRSLAYMRPLSIWAMQLAL 890

[Close]

BLAST of Spo15068.1 vs. ExPASy Swiss-Prot
Match: GBA2_RAT (Non-lysosomal glucosylceramidase OS=Rattus norvegicus GN=Gba2 PE=2 SV=2)

HSP 1 Score: 335.1 bits (858), Expect = 1.100e-90
Identity = 175/360 (48.61%), Postives = 220/360 (61.11%), Query Frame = 1


		  
Query: 1   MWCTYDVHFYASFALLDLFPKIELSIQREFAKAVLSEDGRKVRFLAEGNWGIRKVRGAVP 60
           M+ TYDVHFYASFAL+ L+PK+ELS+Q + A A   ED  + R+L  G     K R  +P
Sbjct: 524 MYNTYDVHFYASFALVMLWPKLELSLQYDMALATFKEDLTRRRYLMSGVVAPVKRRNVIP 583

Query: 61  HDLGTHD--PWKEMNAYNIHDTSKWKDLNPKFVLQVYRDFAATGDMAFGIDVWPSVRAAM 120
           HD+G  D  PW  +NAY IHDT+ WKDLN KFVLQVYRD+  TGD  F  D+WP   A M
Sbjct: 584 HDIGDPDDEPWLRVNAYLIHDTADWKDLNLKFVLQVYRDYYLTGDQGFLKDMWPVCLAVM 643

Query: 121 EYMDQFDMDGDGLIENDGFPDQTYDTWTVHGVSAYCGCLWLAALQAAAAMAFQLGDEEFG 180
           E   +FD D DGLIEN G+ DQTYD W   G SAYCG LWLAA+     MA   G ++  
Sbjct: 644 ESEMKFDKDQDGLIENGGYADQTYDGWVTTGPSAYCGGLWLAAVAVMVQMAVLCGAQDVQ 703

Query: 181 EKYKRKFQMAKHAFEAKLWNGSYFNYDSGSSSNSKSIQADQLAGQWYTASSGL----PNL 240
           +K+       + A+E  LWNG Y+NYDS S   S+S+ +DQ AGQW+  + GL      +
Sbjct: 704 DKFSSILCRGREAYERLLWNGRYYNYDSSSQPQSRSVMSDQCAGQWFLRACGLGEGDTEV 763

Query: 241 FDDFKIKSTLQKFFDYNVMKVKGGRAGAVNGIHPNGKVDETCMQSREIWTGVTYAVAAKM 300
           F    +   L+  F+ NV    GG  GAVNG+ P+G  D + +QS E+W GV Y +AA M
Sbjct: 764 FPTLHVVRALKTIFELNVQAFAGGAMGAVNGMQPHGVPDRSSVQSDEVWVGVVYGLAATM 823

Query: 301 ILAGMEEQAFSTAEGIFIAGWSEDGYGYWFQTPEAWTMDGHFRSLIYMRPLAIWEMQWAL 355
           I  G+  + F TAEG +   W  +  G  FQTPEA+     FRSL YMRPL+IW MQ AL
Sbjct: 824 IQEGLTWEGFRTAEGCYRTVW--ERLGLAFQTPEAYCQQRVFRSLAYMRPLSIWAMQLAL 881

[Close]

BLAST of Spo15068.1 vs. ExPASy Swiss-Prot
Match: C3390_DROME (Non-lysosomal glucosylceramidase OS=Drosophila melanogaster GN=CG33090 PE=1 SV=1)

HSP 1 Score: 195.7 bits (496), Expect = 1.000e-48
Identity = 111/253 (43.87%), Postives = 148/253 (58.50%), Query Frame = 1


		  
Query: 110 VWPSVRAAMEYMDQFDMDGDGLIENDGFPDQTYDTWTVHGVSAYCGCLWLAALQAAAAMA 169
           ++ S +A ME   ++D D DGLIEN   PDQTYD+W + G SAYC  LWLAALQA +AMA
Sbjct: 691 MYASCKAIMERTIEYDKDNDGLIENTKMPDQTYDSWVMDGPSAYCSGLWLAALQAMSAMA 750

Query: 170 FQLGDEEFGEKYKRKFQMAKHAFEAKLWNGSYFNYDSGSSSNSKSIQADQLAGQWYTASS 229
             L       +Y+   +  K + E KLWNGSY+ +D  S S+  +I ADQL G WY  S 
Sbjct: 751 TILDQPNDCLRYQDILEKGKRSLEEKLWNGSYYRFDL-SHSHRDTIMADQLCGHWYLKSC 810

Query: 230 GLP-NLFDDFKIKSTLQKFFDYNVMKVKGGRAGAVNGIHPN-------GKVDETCMQSRE 289
           G    ++    +++ L++ +D NVM    G  GA NG   N       G VD + +Q+ E
Sbjct: 811 GFDYEIYPKENVRTALKRIYDNNVMGFHEGNIGAANGFIANASEPTKPGHVDNSNIQAEE 870

Query: 290 IWTGVTYAVAAKMILAGMEEQAFSTAEGIFIAGWSEDGYGYWFQTPEAWTMDGHFRSLIY 349
           +W GV YA+AA MI  GM E+AF TA G++         G  F+TPEA   +  +RS+ Y
Sbjct: 871 VWPGVVYALAATMIQEGMFEEAFQTAGGMYKT--LSQRIGMNFETPEALYGEKRYRSIGY 930

Query: 350 MRPLAIWEMQWAL 355
           MRPL+IW MQ AL
Sbjct: 931 MRPLSIWSMQVAL 940

[Close]

BLAST of Spo15068.1 vs. TAIR (Arabidopsis)
Match: AT3G24180.1 (Beta-glucosidase, GBA2 type family protein)

HSP 1 Score: 676.8 bits (1745), Expect = 8.400e-195
Identity = 321/407 (78.87%), Postives = 354/407 (86.98%), Query Frame = 1


		  
Query: 1   MWCTYDVHFYASFALLDLFPKIELSIQREFAKAVLSEDGRKVRFLAEGNWGIRKVRGAVP 60
           MWCTYDVHFYAS+ALL LFPKIEL+IQR+FAKAVLSEDGRKV+FLAEGN GIRKVRGAVP
Sbjct: 547 MWCTYDVHFYASYALLMLFPKIELNIQRDFAKAVLSEDGRKVKFLAEGNVGIRKVRGAVP 606

Query: 61  HDLGTHDPWKEMNAYNIHDTSKWKDLNPKFVLQVYRDFAATGDMAFGIDVWPSVRAAMEY 120
           HDLG HDPW EMNAYNIHDTS+WKDLNPKFVLQVYRDFAATGD  FGIDVWP+VRAAMEY
Sbjct: 607 HDLGMHDPWNEMNAYNIHDTSRWKDLNPKFVLQVYRDFAATGDYQFGIDVWPAVRAAMEY 666

Query: 121 MDQFDMDGDGLIENDGFPDQTYDTWTVHGVSAYCGCLWLAALQAAAAMAFQLGDEEFGEK 180
           M+QFD D D LIENDGFPDQTYDTWTVHGVSAYCGCLWLAALQAAAAMA Q+GD+ F E 
Sbjct: 667 MEQFDRDNDDLIENDGFPDQTYDTWTVHGVSAYCGCLWLAALQAAAAMALQIGDKFFAEL 726

Query: 181 YKRKFQMAKHAFEAKLWNGSYFNYDSGSSSNSKSIQADQLAGQWYTASSGLPNLFDDFKI 240
            K KF  AK A E KLWNGSYFNYDSGSSSNSKSIQ DQLAGQWY ASSGLP LF++ KI
Sbjct: 727 CKNKFLNAKAALETKLWNGSYFNYDSGSSSNSKSIQTDQLAGQWYAASSGLPPLFEESKI 786

Query: 241 KSTLQKFFDYNVMKVKGGRAGAVNGIHPNGKVDETCMQSREIWTGVTYAVAAKMILAGME 300
           +ST+QK FD+NVMK KGG+ GAVNG+HP+GKVD+TCMQSREIWTGVTYA AA MIL+GME
Sbjct: 787 RSTMQKIFDFNVMKTKGGKMGAVNGMHPDGKVDDTCMQSREIWTGVTYAAAATMILSGME 846

Query: 301 EQAFSTAEGIFIAGWSEDGYGYWFQTPEAWTMDGHFRSLIYMRPLAIWEMQWALSMPTAV 360
           EQ F+TAEGIF AGWSE+G+GYWFQTPE WTMDGH+RSLIYMRPLAIW MQWALS+P A+
Sbjct: 847 EQGFTTAEGIFTAGWSEEGFGYWFQTPEGWTMDGHYRSLIYMRPLAIWGMQWALSLPKAI 906

Query: 361 IEAPKINIMDRSDLPALSSRSTHNEAGGRKASRKMKCFGNSVFSWAC 408
           ++AP+IN+MDR  L   S R ++N    +    K KCFGNS  S +C
Sbjct: 907 LDAPQINMMDRVHLSPRSRRFSNN---FKVVKHKAKCFGNSALSCSC 950

[Close]

BLAST of Spo15068.1 vs. TAIR (Arabidopsis)
Match: AT5G49900.1 (Beta-glucosidase, GBA2 type family protein)

HSP 1 Score: 484.2 bits (1245), Expect = 8.000e-137
Identity = 238/394 (60.41%), Postives = 288/394 (73.10%), Query Frame = 1


		  
Query: 1   MWCTYDVHFYASFALLDLFPKIELSIQREFAKAVLSEDGRKVRFLAEGNWGIRKVRGAVP 60
           MW TYDVHFYASFAL+ LFPK+ELSIQR+FA AV+  D  KV+ L+EG W  RKV GAVP
Sbjct: 539 MWNTYDVHFYASFALVMLFPKLELSIQRDFAAAVMLHDPTKVKTLSEGQWVQRKVLGAVP 598

Query: 61  HDLGTHDPWKEMNAYNIHDTSKWKDLNPKFVLQVYRDFAATGDMAFGIDVWPSVRAAMEY 120
           HDLG +DPW E+N Y +H+T +WKDLNPKFVLQVYRD  ATGD  F   VWPSV  AM Y
Sbjct: 599 HDLGINDPWFEVNGYTLHNTDRWKDLNPKFVLQVYRDVVATGDKKFASAVWPSVYVAMAY 658

Query: 121 MDQFDMDGDGLIENDGFPDQTYDTWTVHGVSAYCGCLWLAALQAAAAMAFQLGDEEFGEK 180
           M QFD DGDG+IEN+GFPDQTYDTW+  GVSAYCG LW+AALQAA+A+A  +GD+   + 
Sbjct: 659 MAQFDKDGDGMIENEGFPDQTYDTWSASGVSAYCGGLWVAALQAASALARVVGDKNSQDY 718

Query: 181 YKRKFQMAKHAFEAKLWNGSYFNYDSGSSSNSKSIQADQLAGQWYTASSGLPNLFDDFKI 240
           +  KFQ AK  +E KLWNGSYFNYD+  S  S +IQADQLAGQWY  +SGL  + D+ K 
Sbjct: 719 FWSKFQKAKVVYEKKLWNGSYFNYDNSGSQYSSTIQADQLAGQWYARASGLLPIVDEDKA 778

Query: 241 KSTLQKFFDYNVMKVKGGRAGAVNGIHPNGKVDETCMQSREIWTGVTYAVAAKMILAGME 300
           ++ L+K ++YNVMK+K G+ GAVNG+HPNGKVD   MQSREIW+GVTYA++A MI  G+ 
Sbjct: 779 RTALEKVYNYNVMKIKDGKRGAVNGMHPNGKVDTASMQSREIWSGVTYALSATMIQEGLV 838

Query: 301 EQAFSTAEGIFIAGWSEDGYGYWFQTPEAWTMDGHFRSLIYMRPLAIWEMQWALSMPTAV 360
           E AF TA GI+ A WSE G GY FQTPE+W     +RSL YMRPLAIW MQWAL+  +  
Sbjct: 839 EMAFQTASGIYEAAWSETGLGYSFQTPESWNTVDEYRSLTYMRPLAIWAMQWALTKTSQK 898

Query: 361 IEAPKINIMDRSDLPALS-SRSTHNEAGGRKASR 394
            E  ++ +      P L  S S  ++ G  + SR
Sbjct: 899 QE--QLGLEPEQQEPELEPSSSMKHDIGFSRVSR 930

[Close]

BLAST of Spo15068.1 vs. TAIR (Arabidopsis)
Match: AT1G33700.1 (Beta-glucosidase, GBA2 type family protein)

HSP 1 Score: 438.3 bits (1126), Expect = 5.000e-123
Identity = 209/357 (58.54%), Postives = 264/357 (73.95%), Query Frame = 1


		  
Query: 1   MWCTYDVHFYASFALLDLFPKIELSIQREFAKAVLSEDGRKVRFLAEGNWGIRKVRGAVP 60
           M+ TYDVHFY+SFALL LFPKIELSIQR+FA AVL  D  K + ++ G +  RKV GAVP
Sbjct: 532 MYNTYDVHFYSSFALLMLFPKIELSIQRDFAAAVLMHDSSKKQVMSSGEFVTRKVLGAVP 591

Query: 61  HDLGTHDPWKEMNAYNIHDTSKWKDLNPKFVLQVYRDFAATGDMAFGIDVWPSVRAAMEY 120
           HD+G +DPW E+NAYN+ +T +WKDLN KFVLQVYRD  ATGD+ F   VWPSV  A+ Y
Sbjct: 592 HDIGLNDPWFELNAYNLFNTDRWKDLNSKFVLQVYRDVVATGDLNFAKAVWPSVYTAIAY 651

Query: 121 MDQFDMDGDGLIENDGFPDQTYDTWTVHGVSAYCGCLWLAALQAAAAMAFQLGDEEFGEK 180
           +DQFD DGDG+IEN+GFPDQTYD W+  GVSAYCG LW+AALQA +A+A ++GD      
Sbjct: 652 LDQFDKDGDGMIENEGFPDQTYDAWSCSGVSAYCGGLWVAALQAGSALAREIGDNGAAVY 711

Query: 181 YKRKFQMAKHAFEAKLWNGSYFNYDSGSSSNSKSIQADQLAGQWYTASSGLPNLFDDFKI 240
           +  K++ A+  +E KLWNGSYFNYD+  S +S SI ADQ+AGQWY  + GL  +  +  I
Sbjct: 712 FNAKYEKARSVYE-KLWNGSYFNYDNSRSGSSSSILADQMAGQWYARACGLKPIAKEEWI 771

Query: 241 KSTLQKFFDYNVMKVKGGRAGAVNGIHPNGKVDETCMQSREIWTGVTYAVAAKMILAGME 300
           K  L+  +D+NVM+V+ G  GAVNG+ P+G+VD + M SRE+W G TY+VAA MI  G+ 
Sbjct: 772 KKALETVYDFNVMRVRDGTRGAVNGMLPDGRVDTSTMVSREVWAGTTYSVAACMIQEGLA 831

Query: 301 EQAFSTAEGIFIAGWSEDGYGYWFQTPEAWTMDGHFRSLIYMRPLAIWEMQWALSMP 358
           ++ F TA GI+ A WS+ G G  FQTPEAWT +  +RSL YMRPLAIW +QWA +MP
Sbjct: 832 DKGFRTASGIYEAAWSDRGLGCAFQTPEAWTTNDEYRSLCYMRPLAIWGIQWAHTMP 887

[Close]

BLAST of Spo15068.1 vs. TAIR (Arabidopsis)
Match: AT4G10060.1 (Beta-glucosidase, GBA2 type family protein)

HSP 1 Score: 425.6 bits (1093), Expect = 3.400e-119
Identity = 204/355 (57.46%), Postives = 253/355 (71.27%), Query Frame = 1


		  
Query: 1   MWCTYDVHFYASFALLDLFPKIELSIQREFAKAVLSEDGRKVRFLAEGNWGIRKVRGAVP 60
           M+ TYDVHFY+SFALL LFPK+ LSIQR+FA  VL +D  K + ++ G W  RK+ G+VP
Sbjct: 505 MYNTYDVHFYSSFALLSLFPKLALSIQRDFAATVLIQDPTKKKIMSSGEWVTRKLLGSVP 564

Query: 61  HDLGTHDPWKEMNAYNIHDTSKWKDLNPKFVLQVYRDFAATGDMAFGIDVWPSVRAAMEY 120
           HD+G +DPW E+N YN  +T +WKDLN KFVLQVYRD  AT D +F   VWPSV  A+ Y
Sbjct: 565 HDIGLNDPWLELNEYNFFNTDRWKDLNAKFVLQVYRDVVATNDQSFAKAVWPSVYTAVAY 624

Query: 121 MDQFDMDGDGLIENDGFPDQTYDTWTVHGVSAYCGCLWLAALQAAAAMAFQLGDEEFGEK 180
           +DQFD D DG+IEN+GFPDQTYD W+V GVSAYCG LW+AALQAA+A A  +G+      
Sbjct: 625 LDQFDKDEDGMIENEGFPDQTYDAWSVTGVSAYCGGLWVAALQAASAFASIVGENAVAIY 684

Query: 181 YKRKFQMAKHAFEAKLWNGSYFNYDSGSSSNSKSIQADQLAGQWYTASSGLPNLFDDFKI 240
           +  K++ AK  +E KLWNGSYFNYD   S +S SI ADQLAGQWY  + GL  +  +  I
Sbjct: 685 FNAKYEKAKIVYE-KLWNGSYFNYDDSGSGSSSSILADQLAGQWYARACGLKPITKEEWI 744

Query: 241 KSTLQKFFDYNVMKVKGGRAGAVNGIHPNGKVDETCMQSREIWTGVTYAVAAKMILAGME 300
           K  L+  +++NVMKVKGG  GAVNG+   GKVD   + S+E+W G TY+VAA MI  G  
Sbjct: 745 KKALETIYEFNVMKVKGGTRGAVNGMSTEGKVDTNSLVSKEVWAGTTYSVAACMIQEGQR 804

Query: 301 EQAFSTAEGIFIAGWSEDGYGYWFQTPEAWTMDGHFRSLIYMRPLAIWEMQWALS 356
           E+ F TA GI+ A WS+ G    FQTPEAW M+  +RSL YMRPLAIW +QWAL+
Sbjct: 805 EKGFQTASGIYEAIWSDRGLSCSFQTPEAWNMNDEYRSLCYMRPLAIWAIQWALT 858

The following BLAST results are available for this feature:

BLAST of Spo15068.1 vs. NCBI nr
BLAST of Spo15068.1 vs. UniProtKB/TrEMBL
BLAST of Spo15068.1 vs. ExPASy Swiss-Prot
BLAST of Spo15068.1 vs. TAIR (Arabidopsis)

BLAST of Spo15068.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902195156\|gb\|KNA12766.1\|	2.3e-242	99.5	hypothetical protein SOVF_1229...	[more]
gi\|902195155\|gb\|KNA12765.1\|	2.3e-242	99.5	hypothetical protein SOVF_1229...	[more]
gi\|731320443\|ref\|XP_010670809.1\|	6.2e-208	86.4	PREDICTED: non-lysosomal gluco...	[more]
gi\|1012241463\|ref\|XP_015941005.1\|	1.4e-204	83.5	PREDICTED: non-lysosomal gluco...	[more]
gi\|1021567737\|ref\|XP_016175090.1\|	1.4e-204	83.5	PREDICTED: non-lysosomal gluco...	[more]

BLAST of Spo15068.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9QZS3_SPIOL	1.6e-242	99.5	Uncharacterized protein OS=Spi...	[more]
A0A0K9QZS9_SPIOL	1.6e-242	99.5	Uncharacterized protein OS=Spi...	[more]
A0A0J8CT40_BETVU	4.3e-208	86.4	Non-lysosomal glucosylceramida...	[more]
M5Y423_PRUPE	1.7e-204	83.5	Non-lysosomal glucosylceramida...	[more]
A0A0S3SPA3_PHAAN	2.5e-203	82.5	Non-lysosomal glucosylceramida...	[more]

BLAST of Spo15068.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 4


Match Name	E-value	Identity	Description
GBA2_MOUSE	1.5e-92	49.4	Non-lysosomal glucosylceramida...	[more]
GBA2_HUMAN	1.3e-91	49.1	Non-lysosomal glucosylceramida...	[more]
GBA2_RAT	1.1e-90	48.6	Non-lysosomal glucosylceramida...	[more]
C3390_DROME	1.0e-48	43.8	Non-lysosomal glucosylceramida...	[more]

BLAST of Spo15068.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 4


Match Name	E-value	Identity	Description
AT3G24180.1	8.4e-195	78.8	Beta-glucosidase, GBA2 type fa...	[more]
AT5G49900.1	8.0e-137	60.4	Beta-glucosidase, GBA2 type fa...	[more]
AT1G33700.1	5.0e-123	58.5	Beta-glucosidase, GBA2 type fa...	[more]
AT4G10060.1	3.4e-119	57.4	Beta-glucosidase, GBA2 type fa...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006775	Glycosyl-hydrolase family 116, catalytic region	PFAM	PF04685	DUF608	coord: 1..350 score: 2.0E
IPR008928	Six-hairpin glycosidase-like	unknown	SSF48208	Six-hairpin glycosidases	coord: 2..356 score: 1.17
IPR012341	Six-hairpin glycosidase	GENE3D	1.50.10.10		coord: 94..255 score: 2.
None	No IPR available	PANTHER	PTHR12654	BILE ACID BETA-GLUCOSIDASE-RELATED	coord: 1..407 score: 2.2E
None	No IPR available	PANTHER	PTHR12654:SF5	SUBFAMILY NOT NAMED	coord: 1..407 score: 2.2E

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
biological_process	GO:0006680	glucosylceramide catabolic process
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds
molecular_function	GO:0004348	glucosylceramidase activity