Spo16559.1 (mRNA) | SpinachBase

Overview


Name	Spo16559.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	RNA pseudouridine synthase
Location	SpoScf_00596 : 26969 .. 32336 (-)
Sequence length	1197

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTTAGCCTGAAAAGCGGATTTTGGCACACAAAATCGCCCTGAATTTTAGCCCGACATGGCCCACCAGGTCTACCGGAGGCCCGGAGTATTCAAACCGCGCGACCCATGGCTGTTTAGCTACTTATTCATTAAATCCAACGGGTCAAATAATCCTTATCTTCTTCTTCGCATACAAACTTGCCAATTTACTATCAATTAAGATAATAATGCTCGGAAACCCGGTTATAATTGGGGGATTTGGAATTGGGGCGGTGAATTTCGGCCCAATATCAACAAATCCGACAATCTCATATCACCGGTTAATCGGAGCTCCGGCGAAATTCCGGCGGCTTACTTTAGCAGACACTCTTGTCAGGAAGGGCAAATTGGCTGTTTGTTGTATTGCTTCTGCTCCCTCTGCTATTGGGTAATCCAAGTTTCTTCACTCTTATTTTAATTTTAATTATATGTTTATCTGCAATGTAGTTTAATTAGTTTGAAACCCATCAAAAAATTAGTGCAGTAAAATTAGTTCTATCTTGCAATGTTTTATGTTATAGACAATGTACAAATTCTATCTTGCAATGTTTTATGTTATAGACAATGTAAAAATTCTACCTTAGCACCACTCCGGTGCGACTAGAGCTAGGTATACCAAATGGTGGTTTTGGTTATGATTTCATTCTTGATTGACAAAATGGAACAAATTGAGGGTCAAATTAAGTCAACTCTTATTTGTTATGGAATTATTAGGCTTCACTTGTTGAGTAATCTTATGTTATGATGCACTATGAATAAGTGAGGCCCTAAACACCCTCACAATTACTTATAATTGCCTGCACATTTTATGTTATAATCCTTATAGACAATGTACAAATTCTATCCTGCACCCGGGTGCAACTAGCTCGATTTTGGTTACAGTTTCATCCTTGAACTATGTTATGATGTACTATGAATAAATGAGGCTCTAAACACGAAACATTCGCTCGCAATTACTTATAATCTCCTGCACATTTTATGTTATAGACAATGTACAAATTTTATCTTGCACCCGGGTGCGTTAGGTGCACCAAATGATGATTTTGGTTACGGCTTCATCCTTGGTTAACAAAATGGCGGACAATTGAGGGTCAAATCAAGTCGATTCTTATTTGTTATGGAATAGCAGTTTCGGAGTACTATTAGACTTCATTTGTCGAGTAATGTAATTTATATTGTTAATTGGGTTGTTTAATGAAATATTGATAGGAGTGGTGAACTTTATATGAAATTTAACTTAGGAAAGGTTATTGAAAATTTGAAATTGATTATAAGGGTTGGAGTTGAGGGATAGTTTGCACATCTTATTCATAACATTATATGAACATGCTCTTATGCAGCATGAAGTATCACGAAACCAGTACTAGTAGTAGTAGCCTGGCTAGTAGGTAGGTTCATCGACACCTTAGTGAAAGGAAGGAATTTATGACTTTTGATTTTCTCTAGAGATATCCGCACATTGATCCATCTATAACTCAGTGGACTAGTAGTCATACATACAAATATAAAACTACCTCATTGGTGTTACCATATGTCAAGGTTGTTCGACTACTGGTATCACTATAATACGAGCTTGATTGAGAGGAGGTTTTATTGGGTGACTGATTCTTATCCTTTGATGAAAGTGGTTGAAGGAGATATATGGTCTAAGGTAGAGACGCTTCCAACTCTTGCATATTTGAATTTGCTATAGACCGATGCTCATTAATAGGCACATTGGACACCAAACTCGACCATCCATTTCATCCACTAGGTTCATATTAGGCACATTGGAAACACCAAACTCGACCATCCATCAGTGGCGGCACCAATAGGGGTTCAGTTGGTTCAACTGAACCCCTACTGGATTTTAGGAAAGCTTAATTAAAAAAAACCCAGCAAATTAATTTATAAACATTTGCACGCATAACTAATCTTAATGAACCGGTGCAAGTCAGTGGTCCAAACACCACTCAATCTCCAACTGGCTGCGGGATCGATCCCTGCCAGTCTTCTAAGCAAATGTGCCTTCGTGAGTGTTTCTTCATTTTTAGTGTACTCCTAGTTTGTTTTTCCATGTAACGGGTTATTTGAATGATTATTCGGTTGATATTAGGGAAAGGCATATGTTACCTAGTTTTCTTTCCTAACAAATTGTAACTGCAAATTTGCACTAATGGCACATTCACACCCAATATTATGGTTGTAAGCAATTAGGAGAAAGAGATTTTATTGTATTATATATTGATATAGATTAGCTTTTTTTCTTCTTCTTTTAAACCCCAAACCCTTAACGAAAAATTCTTGGGACCGCCACTGCCATCCATTAAGGAAAGTATGTAAGTCAGTGTGGTTTCTTCCAATCCATTTCATCCACTTGGTTCATGTTGTCTCCTTATAGCATCTCCAATGGTTGTATATAGGGACTAGCTTGAAATTTATAAAAGTTTCAAACTAATAGGTAGACCATTGGAGAAAAGTAATAAATTAGCTTGGCCAAGCAAATTAGCTTAAACAAGCTAATTTGCTTGAGAACTTCCAAAAATTGCCAAATAATTAATTGACAAGTGGGACCAATTTAAATAACAAGCTAGTTGCTAGCCATTGGAGCACAAATTAGCTAGACAATGAAATGGCTTGAAATCTTATGTGGCATAACAAGGTAATTGTCAAATTAGCTTACCATTAGAGATGCTCTTATAAACTCCCTGATTCTTTATGGAATAGAGAATGTATTATTCGGTGTACTTGAAAAATTTCCTTAGATCTATCCTTACTTTCAGCAAGTTTCCATGTCTGCCAATCTCGGGGTTTTCAGACAGTTATTAACAGACATATTTTGTTCTAGGCTTTTGCCATTCTTTATTCGATGTCACATCTGACTCTCGTTTCTTATTTTTAGCAACACAAAATATGACCGTCTGTTGCCATATGTTCCAACGAAACACCCACCTAGAGTTGAACACTTGGTTGTCTTAGAAGCAAGCCCTGTTTGTGATTACATTAGCAGAACTTTGAACCTCCCACCTCTGTAAGTTTTTTTTACTGTTTCCTCATTTTTTGTCCTTTCTTTGATGATGAGTGGGAAGAGGTAGTAAACTGAGCTAGTGATTTATGAAGGTATGTTGCTGATCTTATCCATTTTGGAGCTGTTTATTATGCACTCGTACATCCTAAGCCTCCGGCAACTGCAACTCCAGAGCAGGTTCAAGTATATGAAAAATTTACTTCACCTCATCTTCTAAAACAGAGGCAGTCACTTGAGGGGAAAACTGTTTCGGAAGCACAAAAGACCTTCCGTATAACTCATATCGATCAACATGTTGAAGTTGGAACTTACTTGCGCGTATATGTACATCCAAGACGCTTCCCAAGGTTTTTTGAGAATAAAGTTAATTATTTGTGCTTGCAATTTTCCTTTTATGTATAAGTCACACACTGGATTCTTTCAGGTGCTATGAAATCGATTGGAAGTCGAGGATTATAGCTGTTATGGAATCTTATGTGACTCTGGACAAGCCTGCTGGCACTTCTGTAAGTAACTCTTGGAACTACTAGTTACTTAGGTTCTTTATAGTCTTCTGATTAGAAGAGGTATATGCTGTGAATGAAGAAACTAGAATAGATTCATTTATTGAGGACGACTACTTTTTTTTCCTCATCACCACCCCTCTAGGGTGTGCTTTAACTGGTGACATGAAGCATCTAAAGCTTCATGCAGTGGTTAGAAGAATTACCGCTTGGTAAGATTGAGAATATTGTTCAAGCATCTTACGGTCAGTTTGGTAGCCGGTAATAAATGGTAGGAATGGGAATAAAACTAAGTGTTATTTGGCAAGAAAATAACATTATGATGTCCCACCTAATAAATTTGGGAATGAAATTTTGTGTCAAACTTGGTGTTTTCTTCATAAATTTGCATTCCCACCTAATACTACTCCCCAAATGGTAAATGTAATGGAGGGTAATGGAAATTTATAAAGAAAAAGAGATCTTTCTAGTGAATTAGCATTACCATGGAAATGAATATTGATTTTCCTTACAAATTCATTCTCTTTGACACCGTTTATGGCCGGCTACCAAACGGGCCGTTAGGGCTTGAAGTTGGAGTTTTTGTGGCTCTTAACTGATCTTTTGTTCCTCCTTGTATCTTACCTGATCTTCACTCTATTAATTGTTATCTTTTCTTATGGTTTTGTTCCTTGTTATTAAAACATCTATTTTAAGGTAGACGTGTTTATACCTAATATTTTAAGATCATCTGTTTTAAGGTAGACGTGTTTATGCCTAATATTTTAAGGTAGACGTGTTAAATGGGACTGTGGGACATTATCCCATTTCACTTGGAGCTGTGATTATCATCTGATGCAGTCCTTTGAAATGCCTATGTTAATCTCCAAGCTAATATCTGCAATGATGTTTATCTGTCTTTGACTAGGTAGGAGGAACTTCCAACAATATAGAAGAAACTTGTGCGAACTTTGCTACTCGTGCTTTGGGATTGACTAGTCCGCTCTTGACTACACATCAGATTGATAACTGTACTGAAGGATGGTAATATGCTTAGACAGTTGCAGACTATTTTCGTTCAACTGTTTTGGTGCCTAAATCAGCATGTGACCGTCAAACTCATTGCAGTGTTGTGATGGCCAGAACGGAAGATTACTGTTCTATTTTTCATGAAAAAATTAGAGTAAGTTGATACTGTTATCAATAAAGTTTCATATATGATACTCCCTCTGTTCCTAAATAAGTGTCTCATTTGGTGAAAAACACGGGTATTAAGAAATGTGAAAAATGGGTAAGAAAAACAATGGTTGGGAGTGTTTTTATGGGGAAAGGGTAAAGTAGATGATTGTTTATTGATAAAGTACAAATAAAAGTGGAAGTGGGGATGGAAAAGTGTATAATGTGTACGAAAATACAATCATGATTTATGGAAAAGTGGGAAAAATGTATGTCCAAAATTAGAAAAAGGACACTTAAATAGGAAAGCCCTATTAGGAATCCAGGACACCTATTTAGGAACGGAGAGAGTACAATTATTACTGTATTGGACATTGACAATCAAATCCATTTGTACATTATACATGAACTATTGTGACTGATTAATCTTTCTGATGTTTGGTGCAATTAGAGTGTTAGACATCCAACACTAGTACATTTTTGAAAACTCTTACTAAGCTGATATCTAATGTGTTTGTTGCTCTAAATTAGGATAAGAAGGTCAAGAAATACTATCTTGCCCTTGTTGCAGCTCCAGTATCGTGTGGGATAATCACCCATTACATGCAACCGGCAAAGCTTGCTCCACGTATAGTTTCTGAAGGTAACAATCAACATATTAGCCCTTTTGTTTCTTCTTCATAA

mRNA sequence

TTTTAGCCTGAAAAGCGGATTTTGGCACACAAAATCGCCCTGAATTTTAGCCCGACATGGCCCACCAGGTCTACCGGAGGCCCGGAGTATTCAAACCGCGCGACCCATGGCTGTTTAGCTACTTATTCATTAAATCCAACGGGTCAAATAATCCTTATCTTCTTCTTCGCATACAAACTTGCCAATTTACTATCAATTAAGATAATAATGCTCGGAAACCCGGTTATAATTGGGGGATTTGGAATTGGGGCGGTGAATTTCGGCCCAATATCAACAAATCCGACAATCTCATATCACCGGTTAATCGGAGCTCCGGCGAAATTCCGGCGGCTTACTTTAGCAGACACTCTTGTCAGGAAGGGCAAATTGGCTGTTTGTTGTATTGCTTCTGCTCCCTCTGCTATTGGCAACACAAAATATGACCGTCTGTTGCCATATGTTCCAACGAAACACCCACCTAGAGTTGAACACTTGGTTGTCTTAGAAGCAAGCCCTGTTTGTGATTACATTAGCAGAACTTTGAACCTCCCACCTCTGTATGTTGCTGATCTTATCCATTTTGGAGCTGTTTATTATGCACTCGTACATCCTAAGCCTCCGGCAACTGCAACTCCAGAGCAGGTTCAAGTATATGAAAAATTTACTTCACCTCATCTTCTAAAACAGAGGCAGTCACTTGAGGGGAAAACTGTTTCGGAAGCACAAAAGACCTTCCGTATAACTCATATCGATCAACATGTTGAAGTTGGAACTTACTTGCGCGTATATGTACATCCAAGACGCTTCCCAAGGTGCTATGAAATCGATTGGAAGTCGAGGATTATAGCTGTTATGGAATCTTATGTGACTCTGGACAAGCCTGCTGGCACTTCTGTAGGAGGAACTTCCAACAATATAGAAGAAACTTGTGCGAACTTTGCTACTCGTGCTTTGGGATTGACTAGTCCGCTCTTGACTACACATCAGATTGATAACTGTACTGAAGGATGTGTTGTGATGGCCAGAACGGAAGATTACTGTTCTATTTTTCATGAAAAAATTAGAGATAAGAAGGTCAAGAAATACTATCTTGCCCTTGTTGCAGCTCCAGTATCGTGTGGGATAATCACCCATTACATGCAACCGGCAAAGCTTGCTCCACGTATAGTTTCTGAAGGTAACAATCAACATATTAGCCCTTTTGTTTCTTCTTCATAA

Coding sequence (CDS)

ATGCTCGGAAACCCGGTTATAATTGGGGGATTTGGAATTGGGGCGGTGAATTTCGGCCCAATATCAACAAATCCGACAATCTCATATCACCGGTTAATCGGAGCTCCGGCGAAATTCCGGCGGCTTACTTTAGCAGACACTCTTGTCAGGAAGGGCAAATTGGCTGTTTGTTGTATTGCTTCTGCTCCCTCTGCTATTGGCAACACAAAATATGACCGTCTGTTGCCATATGTTCCAACGAAACACCCACCTAGAGTTGAACACTTGGTTGTCTTAGAAGCAAGCCCTGTTTGTGATTACATTAGCAGAACTTTGAACCTCCCACCTCTGTATGTTGCTGATCTTATCCATTTTGGAGCTGTTTATTATGCACTCGTACATCCTAAGCCTCCGGCAACTGCAACTCCAGAGCAGGTTCAAGTATATGAAAAATTTACTTCACCTCATCTTCTAAAACAGAGGCAGTCACTTGAGGGGAAAACTGTTTCGGAAGCACAAAAGACCTTCCGTATAACTCATATCGATCAACATGTTGAAGTTGGAACTTACTTGCGCGTATATGTACATCCAAGACGCTTCCCAAGGTGCTATGAAATCGATTGGAAGTCGAGGATTATAGCTGTTATGGAATCTTATGTGACTCTGGACAAGCCTGCTGGCACTTCTGTAGGAGGAACTTCCAACAATATAGAAGAAACTTGTGCGAACTTTGCTACTCGTGCTTTGGGATTGACTAGTCCGCTCTTGACTACACATCAGATTGATAACTGTACTGAAGGATGTGTTGTGATGGCCAGAACGGAAGATTACTGTTCTATTTTTCATGAAAAAATTAGAGATAAGAAGGTCAAGAAATACTATCTTGCCCTTGTTGCAGCTCCAGTATCGTGTGGGATAATCACCCATTACATGCAACCGGCAAAGCTTGCTCCACGTATAGTTTCTGAAGGTAACAATCAACATATTAGCCCTTTTGTTTCTTCTTCATAA

Protein sequence

MLGNPVIIGGFGIGAVNFGPISTNPTISYHRLIGAPAKFRRLTLADTLVRKGKLAVCCIASAPSAIGNTKYDRLLPYVPTKHPPRVEHLVVLEASPVCDYISRTLNLPPLYVADLIHFGAVYYALVHPKPPATATPEQVQVYEKFTSPHLLKQRQSLEGKTVSEAQKTFRITHIDQHVEVGTYLRVYVHPRRFPRCYEIDWKSRIIAVMESYVTLDKPAGTSVGGTSNNIEETCANFATRALGLTSPLLTTHQIDNCTEGCVVMARTEDYCSIFHEKIRDKKVKKYYLALVAAPVSCGIITHYMQPAKLAPRIVSEGNNQHISPFVSSS

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo16559	Spo16559	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo16559.1	Spo16559.1-protein	polypeptide

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo16559.1.utr5p.1	Spo16559.1.utr5p.1	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo16559.1.CDS.7	Spo16559.1.CDS.7	CDS
Spo16559.1.CDS.6	Spo16559.1.CDS.6	CDS
Spo16559.1.CDS.5	Spo16559.1.CDS.5	CDS
Spo16559.1.CDS.4	Spo16559.1.CDS.4	CDS
Spo16559.1.CDS.3	Spo16559.1.CDS.3	CDS
Spo16559.1.CDS.2	Spo16559.1.CDS.2	CDS
Spo16559.1.CDS.1	Spo16559.1.CDS.1	CDS

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo16559.1.exon.7	Spo16559.1.exon.7	exon
Spo16559.1.exon.6	Spo16559.1.exon.6	exon
Spo16559.1.exon.5	Spo16559.1.exon.5	exon
Spo16559.1.exon.4	Spo16559.1.exon.4	exon
Spo16559.1.exon.3	Spo16559.1.exon.3	exon
Spo16559.1.exon.2	Spo16559.1.exon.2	exon
Spo16559.1.exon.1	Spo16559.1.exon.1	exon

Homology

[Close]

BLAST of Spo16559.1 vs. NCBI nr
Match: gi|902122870|gb|KNA04012.1| (hypothetical protein SOVF_203620 [Spinacia oleracea])

HSP 1 Score: 641.7 bits (1654), Expect = 6.900e-181
Identity = 315/316 (99.68%), Postives = 315/316 (99.68%), Query Frame = 1


		  
Query: 1   MLGNPVIIGGFGIGAVNFGPISTNPTISYHRLIGAPAKFRRLTLADTLVRKGKLAVCCIA 60
           MLGNPVIIGGFGIGAVNFGPISTNPTISYHRLIGAPAKFRRLTLADTLVRKGKLAVCCIA
Sbjct: 1   MLGNPVIIGGFGIGAVNFGPISTNPTISYHRLIGAPAKFRRLTLADTLVRKGKLAVCCIA 60

Query: 61  SAPSAIGNTKYDRLLPYVPTKHPPRVEHLVVLEASPVCDYISRTLNLPPLYVADLIHFGA 120
           SAPSAIGNTKYDRLLPYVPTKHPPRVEHLVVLEASPVCDYISRTLNLPPLYVADLIHFGA
Sbjct: 61  SAPSAIGNTKYDRLLPYVPTKHPPRVEHLVVLEASPVCDYISRTLNLPPLYVADLIHFGA 120

Query: 121 VYYALVHPKPPATATPEQVQVYEKFTSPHLLKQRQSLEGKTVSEAQKTFRITHIDQHVEV 180
           VYYALVHPKPPATATPEQVQVYEKFTSPHLLKQRQSLEGKTVSEAQKTFRITHIDQHVEV
Sbjct: 121 VYYALVHPKPPATATPEQVQVYEKFTSPHLLKQRQSLEGKTVSEAQKTFRITHIDQHVEV 180

Query: 181 GTYLRVYVHPRRFPRCYEIDWKSRIIAVMESYVTLDKPAGTSVGGTSNNIEETCANFATR 240
           GTYLRVYVHPRRFPRCYEIDWKSRIIAVMESYV LDKPAGTSVGGTSNNIEETCANFATR
Sbjct: 181 GTYLRVYVHPRRFPRCYEIDWKSRIIAVMESYVILDKPAGTSVGGTSNNIEETCANFATR 240

Query: 241 ALGLTSPLLTTHQIDNCTEGCVVMARTEDYCSIFHEKIRDKKVKKYYLALVAAPVSCGII 300
           ALGLTSPLLTTHQIDNCTEGCVVMARTEDYCSIFHEKIRDKKVKKYYLALVAAPVSCGII
Sbjct: 241 ALGLTSPLLTTHQIDNCTEGCVVMARTEDYCSIFHEKIRDKKVKKYYLALVAAPVSCGII 300

Query: 301 THYMQPAKLAPRIVSE 317
           THYMQPAKLAPRIVSE
Sbjct: 301 THYMQPAKLAPRIVSE 316

[Close]

BLAST of Spo16559.1 vs. NCBI nr
Match: gi|731369863|ref|XP_010665548.1| (PREDICTED: RNA pseudouridine synthase 6, chloroplastic-like [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 479.6 bits (1233), Expect = 4.500e-132
Identity = 245/322 (76.09%), Postives = 268/322 (83.23%), Query Frame = 1


		  
Query: 1   MLGNPVIIGGFGIGAVNFGPISTNPTISYHRL-------IGAPAKFRRLTLADTLVRKGK 60
           MLGN +      IGAVN+ PISTN  IS  R+       IGAPA     TL   LV   +
Sbjct: 1   MLGNQL-----AIGAVNYLPISTNTPISPRRVQTNHIHFIGAPA-----TLRCPLVGNAE 60

Query: 61  LAVCCIASAPSAIGNTKYDRLLPYVPTKHPPRVEHLVVLEASPVCDYISRTLNLPPLYVA 120
            A+ C+ASAPS  G  +YDRLLP   T HPPR+EHLVVL+   V DYISRTL+LP LYVA
Sbjct: 61  SAIYCVASAPSTNGYPEYDRLLPCPSTTHPPRIEHLVVLKEGSVGDYISRTLDLPALYVA 120

Query: 121 DLIHFGAVYYALVHPKPPATATPEQVQVYEKFTSPHLLKQRQSLEGKTVSEAQKTFRITH 180
           DLIHFGAVYYALVHP+PP TATPEQVQVYE FTSP LLK+R+SL+GKT+ EAQ+TFRITH
Sbjct: 121 DLIHFGAVYYALVHPEPPTTATPEQVQVYENFTSPLLLKKRRSLKGKTIPEAQRTFRITH 180

Query: 181 IDQHVEVGTYLRVYVHPRRFPRCYEIDWKSRIIAVMESYVTLDKPAGTSVGGTSNNIEET 240
           +D+HVEVGTYLRVYVHPRRFPRCYEIDWK RIIA+ ESYV LDKPAGTSVGGTSNNIEE+
Sbjct: 181 VDEHVEVGTYLRVYVHPRRFPRCYEIDWKLRIIAITESYVVLDKPAGTSVGGTSNNIEES 240

Query: 241 CANFATRALGLTSPLLTTHQIDNCTEGCVVMARTEDYCSIFHEKIRDKKVKKYYLALVAA 300
           CANFATRALGL SPLLTTHQIDNCTEGCVVMARTEDYCS+FHEKIRDKKVKK YLALVA+
Sbjct: 241 CANFATRALGLASPLLTTHQIDNCTEGCVVMARTEDYCSVFHEKIRDKKVKKLYLALVAS 300

Query: 301 PVSCGIITHYMQPAKLAPRIVS 316
           PVSCGIITHYMQPAKLAPRI+S
Sbjct: 301 PVSCGIITHYMQPAKLAPRILS 312

[Close]

BLAST of Spo16559.1 vs. NCBI nr
Match: gi|731369849|ref|XP_010665544.1| (PREDICTED: RNA pseudouridine synthase 6, chloroplastic [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 408.7 bits (1049), Expect = 9.800e-111
Identity = 207/328 (63.11%), Postives = 247/328 (75.30%), Query Frame = 1


		  
Query: 14  GAVNFGPISTNPTI-----SYHRLIGAPAKFRRLTLADTLVRKGKL----AVCCIAS--- 73
           GA N G    N        ++ R+ GAP+ FRR       V + KL      C ++S   
Sbjct: 4   GATNVGSNFINAMFLSLQKTHFRVFGAPSTFRRTLATRHHVSRKKLRREKVSCSVSSFRS 63

Query: 74  -------------APSAIGNTKYDRLLPYVPTKHPPRVEHLVVLEASPVCDYISRTLNLP 133
                        AP   G  +YDRLLP      PPRVEHLVVLE SPVC+YIS++L+LP
Sbjct: 64  QDVTSTAATITSPAPFVNGYPEYDRLLPCPSENMPPRVEHLVVLEESPVCEYISKSLDLP 123

Query: 134 PLYVADLIHFGAVYYALVHPKPPATATPEQVQVYEKFTSPHLLKQRQSLEGKTVSEAQKT 193
           PLYVADLIHFGAVYYA V P+PP TATPEQ+Q+YE FTSP LLK+R+S +GKTV EAQKT
Sbjct: 124 PLYVADLIHFGAVYYAHVCPEPPVTATPEQIQLYEHFTSPELLKERRSFKGKTVREAQKT 183

Query: 194 FRITHIDQHVEVGTYLRVYVHPRRFPRCYEIDWKSRIIAVMESYVTLDKPAGTSVGGTSN 253
            R+TH D+++E GTYLRV+VHP+RFPRCYEIDWK+RI+AV +SYV LDKPAGTSVGG+S+
Sbjct: 184 LRVTHPDEYLEAGTYLRVHVHPKRFPRCYEIDWKARILAVTDSYVILDKPAGTSVGGSSD 243

Query: 254 NIEETCANFATRALGLTSPLLTTHQIDNCTEGCVVMARTEDYCSIFHEKIRDKKVKKYYL 313
           NIEE+CANFATRALGLTSPL TTHQIDNCTEGCVV+A++++YCS FH+KIRDK VKK YL
Sbjct: 244 NIEESCANFATRALGLTSPLFTTHQIDNCTEGCVVLAKSKEYCSTFHKKIRDKLVKKLYL 303

Query: 314 ALVAAPVSCGIITHYMQPAKLAPRIVSE 317
           AL AAPV  GI+THYM+PA LAPR++SE
Sbjct: 304 ALAAAPVPRGILTHYMRPANLAPRLISE 331

[Close]

BLAST of Spo16559.1 vs. NCBI nr
Match: gi|902187681|gb|KNA10963.1| (hypothetical protein SOVF_139500, partial [Spinacia oleracea])

HSP 1 Score: 402.1 bits (1032), Expect = 9.100e-109
Identity = 204/330 (61.82%), Postives = 249/330 (75.45%), Query Frame = 1


		  
Query: 3   GNPVIIGGFGIGAVNFGPISTNPTISYHRLIGAPAKFRRLTLADTLVRKGKLA---VCC- 62
           GNP+ +G    G+     + ++   ++ RL GAP    R    +  + K K+    + C 
Sbjct: 13  GNPMSLGASNSGSNFINALFSSLRRTHFRLAGAPTTLWRTLATNRPISKNKVKREKINCS 72

Query: 63  ----------IASAPSAIGN--TKYDRLLPYVPTKHPPRVEHLVVLEASPVCDYISRTLN 122
                     I S+ S++ N   +Y RLLP   T  PPRVEHLVVLE SPVC+YIS++L+
Sbjct: 73  VSVFNNEASSITSSTSSVNNGYPEYSRLLPSPSTNAPPRVEHLVVLEESPVCEYISKSLD 132

Query: 123 LPPLYVADLIHFGAVYYALVHPKPPATATPEQVQVYEKFTSPHLLKQRQSLEGKTVSEAQ 182
           LPPLYVADLIHFGAVYYAL+ P PP TATPEQV +Y++FTSP LLKQR+S +GKT  EAQ
Sbjct: 133 LPPLYVADLIHFGAVYYALICPDPPVTATPEQVVLYKQFTSPELLKQRRSTKGKTFREAQ 192

Query: 183 KTFRITHIDQHVEVGTYLRVYVHPRRFPRCYEIDWKSRIIAVMESYVTLDKPAGTSVGGT 242
           KTFR+T I+++ EVGTYLRV+VHP+RFPRCYE+DW SR+IAV ESYV L+KPAGTSVGG+
Sbjct: 193 KTFRVTRIEENFEVGTYLRVHVHPKRFPRCYEVDWMSRVIAVTESYVVLNKPAGTSVGGS 252

Query: 243 SNNIEETCANFATRALGLTSPLLTTHQIDNCTEGCVVMARTEDYCSIFHEKIRDKKVKKY 302
           S+NIEE+CANF TRALGLTSPL TTHQIDNCTEGC+V+AR ++YCS FH+KIRDK VKK 
Sbjct: 253 SDNIEESCANFTTRALGLTSPLYTTHQIDNCTEGCLVLARDKEYCSKFHKKIRDKMVKKL 312

Query: 303 YLALVAAPVSCGIITHYMQPAKLAPRIVSE 317
           YLAL AAPV  GI THYM+PA LAPR+VSE
Sbjct: 313 YLALAAAPVPRGIYTHYMRPANLAPRLVSE 342

[Close]

BLAST of Spo16559.1 vs. NCBI nr
Match: gi|778658473|ref|XP_011652789.1| (PREDICTED: RNA pseudouridine synthase 6, chloroplastic isoform X3 [Cucumis sativus])

HSP 1 Score: 394.0 bits (1011), Expect = 2.500e-106
Identity = 188/250 (75.20%), Postives = 217/250 (86.80%), Query Frame = 1


		  
Query: 67  GNTKYDRLLPYVPTKHPPRVEHLVVLEASPVCDYISRTLNLPPLYVADLIHFGAVYYALV 126
           G  +Y RLLP      PPRVEH+VVLEA PV +YI ++LNLPPLYVADLIHFGAVYYALV
Sbjct: 75  GYPQYHRLLPCPSFSKPPRVEHMVVLEAGPVMEYICKSLNLPPLYVADLIHFGAVYYALV 134

Query: 127 HPKPPATATPEQVQVYEKFTSPHLLKQRQSLEGKTVSEAQKTFRITHIDQHVEVGTYLRV 186
            P+PP TATPEQ+++++KFT P  LK R+S++GKTV EAQKTFRITH+D+ VEVGTYLRV
Sbjct: 135 CPQPPKTATPEQIRLFKKFTEPSFLKGRKSIKGKTVREAQKTFRITHVDEFVEVGTYLRV 194

Query: 187 YVHPRRFPRCYEIDWKSRIIAVMESYVTLDKPAGTSVGGTSNNIEETCANFATRALGLTS 246
           +VHP+RFPRCYEIDWKSRIIAV ESYV LDKPAGTSVGGT++NIEE+CA FATRALGLTS
Sbjct: 195 HVHPKRFPRCYEIDWKSRIIAVTESYVVLDKPAGTSVGGTTDNIEESCATFATRALGLTS 254

Query: 247 PLLTTHQIDNCTEGCVVMARTEDYCSIFHEKIRDKKVKKYYLALVAAPVSCGIITHYMQP 306
           PL TTHQIDNCTEGCV+ ART++YCSIFH KIR+KKV K YLAL AAPV  GIITHYM+P
Sbjct: 255 PLWTTHQIDNCTEGCVIFARTKEYCSIFHGKIREKKVMKLYLALSAAPVPMGIITHYMRP 314

Query: 307 AKLAPRIVSE 317
             +APR+VS+
Sbjct: 315 INIAPRLVSK 324

[Close]

BLAST of Spo16559.1 vs. UniProtKB/TrEMBL
Match: A0A0K9Q9P9_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_203620 PE=4 SV=1)

HSP 1 Score: 641.7 bits (1654), Expect = 4.800e-181
Identity = 315/316 (99.68%), Postives = 315/316 (99.68%), Query Frame = 1


		  
Query: 1   MLGNPVIIGGFGIGAVNFGPISTNPTISYHRLIGAPAKFRRLTLADTLVRKGKLAVCCIA 60
           MLGNPVIIGGFGIGAVNFGPISTNPTISYHRLIGAPAKFRRLTLADTLVRKGKLAVCCIA
Sbjct: 1   MLGNPVIIGGFGIGAVNFGPISTNPTISYHRLIGAPAKFRRLTLADTLVRKGKLAVCCIA 60

Query: 61  SAPSAIGNTKYDRLLPYVPTKHPPRVEHLVVLEASPVCDYISRTLNLPPLYVADLIHFGA 120
           SAPSAIGNTKYDRLLPYVPTKHPPRVEHLVVLEASPVCDYISRTLNLPPLYVADLIHFGA
Sbjct: 61  SAPSAIGNTKYDRLLPYVPTKHPPRVEHLVVLEASPVCDYISRTLNLPPLYVADLIHFGA 120

Query: 121 VYYALVHPKPPATATPEQVQVYEKFTSPHLLKQRQSLEGKTVSEAQKTFRITHIDQHVEV 180
           VYYALVHPKPPATATPEQVQVYEKFTSPHLLKQRQSLEGKTVSEAQKTFRITHIDQHVEV
Sbjct: 121 VYYALVHPKPPATATPEQVQVYEKFTSPHLLKQRQSLEGKTVSEAQKTFRITHIDQHVEV 180

Query: 181 GTYLRVYVHPRRFPRCYEIDWKSRIIAVMESYVTLDKPAGTSVGGTSNNIEETCANFATR 240
           GTYLRVYVHPRRFPRCYEIDWKSRIIAVMESYV LDKPAGTSVGGTSNNIEETCANFATR
Sbjct: 181 GTYLRVYVHPRRFPRCYEIDWKSRIIAVMESYVILDKPAGTSVGGTSNNIEETCANFATR 240

Query: 241 ALGLTSPLLTTHQIDNCTEGCVVMARTEDYCSIFHEKIRDKKVKKYYLALVAAPVSCGII 300
           ALGLTSPLLTTHQIDNCTEGCVVMARTEDYCSIFHEKIRDKKVKKYYLALVAAPVSCGII
Sbjct: 241 ALGLTSPLLTTHQIDNCTEGCVVMARTEDYCSIFHEKIRDKKVKKYYLALVAAPVSCGII 300

Query: 301 THYMQPAKLAPRIVSE 317
           THYMQPAKLAPRIVSE
Sbjct: 301 THYMQPAKLAPRIVSE 316

[Close]

BLAST of Spo16559.1 vs. UniProtKB/TrEMBL
Match: A0A0J8E0U7_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_8g200350 PE=4 SV=1)

HSP 1 Score: 479.6 bits (1233), Expect = 3.100e-132
Identity = 245/322 (76.09%), Postives = 268/322 (83.23%), Query Frame = 1


		  
Query: 1   MLGNPVIIGGFGIGAVNFGPISTNPTISYHRL-------IGAPAKFRRLTLADTLVRKGK 60
           MLGN +      IGAVN+ PISTN  IS  R+       IGAPA     TL   LV   +
Sbjct: 1   MLGNQL-----AIGAVNYLPISTNTPISPRRVQTNHIHFIGAPA-----TLRCPLVGNAE 60

Query: 61  LAVCCIASAPSAIGNTKYDRLLPYVPTKHPPRVEHLVVLEASPVCDYISRTLNLPPLYVA 120
            A+ C+ASAPS  G  +YDRLLP   T HPPR+EHLVVL+   V DYISRTL+LP LYVA
Sbjct: 61  SAIYCVASAPSTNGYPEYDRLLPCPSTTHPPRIEHLVVLKEGSVGDYISRTLDLPALYVA 120

Query: 121 DLIHFGAVYYALVHPKPPATATPEQVQVYEKFTSPHLLKQRQSLEGKTVSEAQKTFRITH 180
           DLIHFGAVYYALVHP+PP TATPEQVQVYE FTSP LLK+R+SL+GKT+ EAQ+TFRITH
Sbjct: 121 DLIHFGAVYYALVHPEPPTTATPEQVQVYENFTSPLLLKKRRSLKGKTIPEAQRTFRITH 180

Query: 181 IDQHVEVGTYLRVYVHPRRFPRCYEIDWKSRIIAVMESYVTLDKPAGTSVGGTSNNIEET 240
           +D+HVEVGTYLRVYVHPRRFPRCYEIDWK RIIA+ ESYV LDKPAGTSVGGTSNNIEE+
Sbjct: 181 VDEHVEVGTYLRVYVHPRRFPRCYEIDWKLRIIAITESYVVLDKPAGTSVGGTSNNIEES 240

Query: 241 CANFATRALGLTSPLLTTHQIDNCTEGCVVMARTEDYCSIFHEKIRDKKVKKYYLALVAA 300
           CANFATRALGL SPLLTTHQIDNCTEGCVVMARTEDYCS+FHEKIRDKKVKK YLALVA+
Sbjct: 241 CANFATRALGLASPLLTTHQIDNCTEGCVVMARTEDYCSVFHEKIRDKKVKKLYLALVAS 300

Query: 301 PVSCGIITHYMQPAKLAPRIVS 316
           PVSCGIITHYMQPAKLAPRI+S
Sbjct: 301 PVSCGIITHYMQPAKLAPRILS 312

[Close]

BLAST of Spo16559.1 vs. UniProtKB/TrEMBL
Match: A0A0J8B9P8_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_8g200330 PE=4 SV=1)

HSP 1 Score: 408.7 bits (1049), Expect = 6.800e-111
Identity = 207/328 (63.11%), Postives = 247/328 (75.30%), Query Frame = 1


		  
Query: 14  GAVNFGPISTNPTI-----SYHRLIGAPAKFRRLTLADTLVRKGKL----AVCCIAS--- 73
           GA N G    N        ++ R+ GAP+ FRR       V + KL      C ++S   
Sbjct: 4   GATNVGSNFINAMFLSLQKTHFRVFGAPSTFRRTLATRHHVSRKKLRREKVSCSVSSFRS 63

Query: 74  -------------APSAIGNTKYDRLLPYVPTKHPPRVEHLVVLEASPVCDYISRTLNLP 133
                        AP   G  +YDRLLP      PPRVEHLVVLE SPVC+YIS++L+LP
Sbjct: 64  QDVTSTAATITSPAPFVNGYPEYDRLLPCPSENMPPRVEHLVVLEESPVCEYISKSLDLP 123

Query: 134 PLYVADLIHFGAVYYALVHPKPPATATPEQVQVYEKFTSPHLLKQRQSLEGKTVSEAQKT 193
           PLYVADLIHFGAVYYA V P+PP TATPEQ+Q+YE FTSP LLK+R+S +GKTV EAQKT
Sbjct: 124 PLYVADLIHFGAVYYAHVCPEPPVTATPEQIQLYEHFTSPELLKERRSFKGKTVREAQKT 183

Query: 194 FRITHIDQHVEVGTYLRVYVHPRRFPRCYEIDWKSRIIAVMESYVTLDKPAGTSVGGTSN 253
            R+TH D+++E GTYLRV+VHP+RFPRCYEIDWK+RI+AV +SYV LDKPAGTSVGG+S+
Sbjct: 184 LRVTHPDEYLEAGTYLRVHVHPKRFPRCYEIDWKARILAVTDSYVILDKPAGTSVGGSSD 243

Query: 254 NIEETCANFATRALGLTSPLLTTHQIDNCTEGCVVMARTEDYCSIFHEKIRDKKVKKYYL 313
           NIEE+CANFATRALGLTSPL TTHQIDNCTEGCVV+A++++YCS FH+KIRDK VKK YL
Sbjct: 244 NIEESCANFATRALGLTSPLFTTHQIDNCTEGCVVLAKSKEYCSTFHKKIRDKLVKKLYL 303

Query: 314 ALVAAPVSCGIITHYMQPAKLAPRIVSE 317
           AL AAPV  GI+THYM+PA LAPR++SE
Sbjct: 304 ALAAAPVPRGILTHYMRPANLAPRLISE 331

[Close]

BLAST of Spo16559.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QW75_SPIOL (Uncharacterized protein (Fragment) OS=Spinacia oleracea GN=SOVF_139500 PE=4 SV=1)

HSP 1 Score: 402.1 bits (1032), Expect = 6.400e-109
Identity = 204/330 (61.82%), Postives = 249/330 (75.45%), Query Frame = 1


		  
Query: 3   GNPVIIGGFGIGAVNFGPISTNPTISYHRLIGAPAKFRRLTLADTLVRKGKLA---VCC- 62
           GNP+ +G    G+     + ++   ++ RL GAP    R    +  + K K+    + C 
Sbjct: 13  GNPMSLGASNSGSNFINALFSSLRRTHFRLAGAPTTLWRTLATNRPISKNKVKREKINCS 72

Query: 63  ----------IASAPSAIGN--TKYDRLLPYVPTKHPPRVEHLVVLEASPVCDYISRTLN 122
                     I S+ S++ N   +Y RLLP   T  PPRVEHLVVLE SPVC+YIS++L+
Sbjct: 73  VSVFNNEASSITSSTSSVNNGYPEYSRLLPSPSTNAPPRVEHLVVLEESPVCEYISKSLD 132

Query: 123 LPPLYVADLIHFGAVYYALVHPKPPATATPEQVQVYEKFTSPHLLKQRQSLEGKTVSEAQ 182
           LPPLYVADLIHFGAVYYAL+ P PP TATPEQV +Y++FTSP LLKQR+S +GKT  EAQ
Sbjct: 133 LPPLYVADLIHFGAVYYALICPDPPVTATPEQVVLYKQFTSPELLKQRRSTKGKTFREAQ 192

Query: 183 KTFRITHIDQHVEVGTYLRVYVHPRRFPRCYEIDWKSRIIAVMESYVTLDKPAGTSVGGT 242
           KTFR+T I+++ EVGTYLRV+VHP+RFPRCYE+DW SR+IAV ESYV L+KPAGTSVGG+
Sbjct: 193 KTFRVTRIEENFEVGTYLRVHVHPKRFPRCYEVDWMSRVIAVTESYVVLNKPAGTSVGGS 252

Query: 243 SNNIEETCANFATRALGLTSPLLTTHQIDNCTEGCVVMARTEDYCSIFHEKIRDKKVKKY 302
           S+NIEE+CANF TRALGLTSPL TTHQIDNCTEGC+V+AR ++YCS FH+KIRDK VKK 
Sbjct: 253 SDNIEESCANFTTRALGLTSPLYTTHQIDNCTEGCLVLARDKEYCSKFHKKIRDKMVKKL 312

Query: 303 YLALVAAPVSCGIITHYMQPAKLAPRIVSE 317
           YLAL AAPV  GI THYM+PA LAPR+VSE
Sbjct: 313 YLALAAAPVPRGIYTHYMRPANLAPRLVSE 342

[Close]

BLAST of Spo16559.1 vs. UniProtKB/TrEMBL
Match: A0A0A0LX47_CUCSA (Uncharacterized protein OS=Cucumis sativus GN=Csa_1G064140 PE=4 SV=1)

HSP 1 Score: 394.0 bits (1011), Expect = 1.700e-106
Identity = 188/250 (75.20%), Postives = 217/250 (86.80%), Query Frame = 1


		  
Query: 67  GNTKYDRLLPYVPTKHPPRVEHLVVLEASPVCDYISRTLNLPPLYVADLIHFGAVYYALV 126
           G  +Y RLLP      PPRVEH+VVLEA PV +YI ++LNLPPLYVADLIHFGAVYYALV
Sbjct: 79  GYPQYHRLLPCPSFSKPPRVEHMVVLEAGPVMEYICKSLNLPPLYVADLIHFGAVYYALV 138

Query: 127 HPKPPATATPEQVQVYEKFTSPHLLKQRQSLEGKTVSEAQKTFRITHIDQHVEVGTYLRV 186
            P+PP TATPEQ+++++KFT P  LK R+S++GKTV EAQKTFRITH+D+ VEVGTYLRV
Sbjct: 139 CPQPPKTATPEQIRLFKKFTEPSFLKGRKSIKGKTVREAQKTFRITHVDEFVEVGTYLRV 198

Query: 187 YVHPRRFPRCYEIDWKSRIIAVMESYVTLDKPAGTSVGGTSNNIEETCANFATRALGLTS 246
           +VHP+RFPRCYEIDWKSRIIAV ESYV LDKPAGTSVGGT++NIEE+CA FATRALGLTS
Sbjct: 199 HVHPKRFPRCYEIDWKSRIIAVTESYVVLDKPAGTSVGGTTDNIEESCATFATRALGLTS 258

Query: 247 PLLTTHQIDNCTEGCVVMARTEDYCSIFHEKIRDKKVKKYYLALVAAPVSCGIITHYMQP 306
           PL TTHQIDNCTEGCV+ ART++YCSIFH KIR+KKV K YLAL AAPV  GIITHYM+P
Sbjct: 259 PLWTTHQIDNCTEGCVIFARTKEYCSIFHGKIREKKVMKLYLALSAAPVPMGIITHYMRP 318

Query: 307 AKLAPRIVSE 317
             +APR+VS+
Sbjct: 319 INIAPRLVSK 328

[Close]

BLAST of Spo16559.1 vs. ExPASy Swiss-Prot
Match: PUS6_ARATH (RNA pseudouridine synthase 6, chloroplastic OS=Arabidopsis thaliana GN=At4g21770 PE=2 SV=1)

HSP 1 Score: 356.7 bits (914), Expect = 2.800e-97
Identity = 177/291 (60.82%), Postives = 225/291 (77.32%), Query Frame = 1


		  
Query: 31  RLIGAPAKFRRLTLADTLVRKGKLAVCCIASAPSAIGNTKYDRLLPYVPTKHPPRVEHLV 90
           R I +P +F  L+L  T  +          S+ S  G  +Y+RL+P      PPR+EH+V
Sbjct: 41  RFISSPKRFTCLSLLKTDSQNQTTL-----SSSSNSGYHEYNRLMPCPAYNLPPRIEHMV 100

Query: 91  VLEASP-VCDYISRTLNLPPLYVADLIHFGAVYYALVHPKPPATATPEQVQVYEKFTSPH 150
           VLE    V ++IS+ L+LPPLYVADLI FGAV+YALV PKPP TATPE++ ++E+ TSP 
Sbjct: 101 VLEDDVLVSEFISKQLDLPPLYVADLIRFGAVHYALVCPKPPPTATPEEIILFEEVTSPS 160

Query: 151 LLKQRQSLEGKTVSEAQKTFRITHIDQHVEVGTYLRVYVHPRRFPRCYEIDWKSRIIAVM 210
           +LK+R S++GKTV EAQKTFR+TH +Q+ E GTYLRV+VHP+R PRCYEIDWKSRI+AV 
Sbjct: 161 VLKKRSSIKGKTVREAQKTFRVTHTNQYAEAGTYLRVHVHPKRSPRCYEIDWKSRIVAVT 220

Query: 211 ESYVTLDKPAGTSVGGTSNNIEETCANFATRALGLTSPLLTTHQIDNCTEGCVVMARTED 270
           +SYV LDKPAGT+VGGT++NIEE+CA FA+RAL L  PL TTHQIDNCTEGCVV ART++
Sbjct: 221 DSYVILDKPAGTTVGGTTDNIEESCATFASRALDLPEPLKTTHQIDNCTEGCVVFARTKE 280

Query: 271 YCSIFHEKIRDKKVKKYYLALVAAPVSCGIITHYMQPAKLAPRIVSEGNNQ 321
           YCS+FH KIR+K+VKK Y AL AAP+  GII+HYM+P  +APR+V+E + Q
Sbjct: 281 YCSVFHTKIRNKEVKKLYRALAAAPLPIGIISHYMRPKNMAPRLVAEDSIQ 326

[Close]

BLAST of Spo16559.1 vs. ExPASy Swiss-Prot
Match: PUS6_ORYSJ (RNA pseudouridine synthase 6, chloroplastic OS=Oryza sativa subsp. japonica GN=Os07g0660400 PE=2 SV=1)

HSP 1 Score: 338.2 bits (866), Expect = 1.000e-91
Identity = 160/255 (62.75%), Postives = 200/255 (78.43%), Query Frame = 1


		  
Query: 62  APSAIGNTKYDRLLPYVPTKHPPRVEHLVVLEASPVCDYISRTLNLPPLYVADLIHFGAV 121
           AP+      Y RLLP      PPR+EHLV  E     D+ISR+L LPPLYVADLI FGAV
Sbjct: 65  APATAAYPVYGRLLPCPLQDDPPRIEHLVAREDEVAVDFISRSLTLPPLYVADLIKFGAV 124

Query: 122 YYALVHPKPPATATPEQVQVYEKFTSPHLLKQRQSLEGKTVSEAQKTFRITHIDQHVEVG 181
           YYALV P+PP  A PE V+++ + T P +L +R+S++GKTV EAQKTFR+T  +Q +E G
Sbjct: 125 YYALVAPQPPPHAAPEHVRIFREVTEPSVLCRRKSIKGKTVREAQKTFRVTDPNQRLEAG 184

Query: 182 TYLRVYVHPRRFPRCYEIDWKSRIIAVMESYVTLDKPAGTSVGGTSNNIEETCANFATRA 241
           TYLRV+VHP+RFPRCYEIDWKSR+IAV ++YV LDKPA TSVGG ++NIEE+C  F +RA
Sbjct: 185 TYLRVHVHPKRFPRCYEIDWKSRVIAVTDNYVVLDKPAATSVGGATDNIEESCVVFTSRA 244

Query: 242 LGLTSPLLTTHQIDNCTEGCVVMARTEDYCSIFHEKIRDKKVKKYYLALVAAPVSCGIIT 301
           LGL +PL+TTHQIDNC+EGCVV+++T+++CS+FH  IR+K+V K YLAL  APVS GIIT
Sbjct: 245 LGLETPLMTTHQIDNCSEGCVVLSKTKEFCSVFHGMIREKQVNKRYLALTTAPVSTGIIT 304

Query: 302 HYMQPAKLAPRIVSE 317
           HYM+P   APR+VSE
Sbjct: 305 HYMRPINRAPRLVSE 319

[Close]

BLAST of Spo16559.1 vs. TAIR (Arabidopsis)
Match: AT4G21770.1 (Pseudouridine synthase family protein)

HSP 1 Score: 356.7 bits (914), Expect = 1.600e-98
Identity = 177/291 (60.82%), Postives = 225/291 (77.32%), Query Frame = 1


		  
Query: 31  RLIGAPAKFRRLTLADTLVRKGKLAVCCIASAPSAIGNTKYDRLLPYVPTKHPPRVEHLV 90
           R I +P +F  L+L  T  +          S+ S  G  +Y+RL+P      PPR+EH+V
Sbjct: 41  RFISSPKRFTCLSLLKTDSQNQTTL-----SSSSNSGYHEYNRLMPCPAYNLPPRIEHMV 100

Query: 91  VLEASP-VCDYISRTLNLPPLYVADLIHFGAVYYALVHPKPPATATPEQVQVYEKFTSPH 150
           VLE    V ++IS+ L+LPPLYVADLI FGAV+YALV PKPP TATPE++ ++E+ TSP 
Sbjct: 101 VLEDDVLVSEFISKQLDLPPLYVADLIRFGAVHYALVCPKPPPTATPEEIILFEEVTSPS 160

Query: 151 LLKQRQSLEGKTVSEAQKTFRITHIDQHVEVGTYLRVYVHPRRFPRCYEIDWKSRIIAVM 210
           +LK+R S++GKTV EAQKTFR+TH +Q+ E GTYLRV+VHP+R PRCYEIDWKSRI+AV 
Sbjct: 161 VLKKRSSIKGKTVREAQKTFRVTHTNQYAEAGTYLRVHVHPKRSPRCYEIDWKSRIVAVT 220

Query: 211 ESYVTLDKPAGTSVGGTSNNIEETCANFATRALGLTSPLLTTHQIDNCTEGCVVMARTED 270
           +SYV LDKPAGT+VGGT++NIEE+CA FA+RAL L  PL TTHQIDNCTEGCVV ART++
Sbjct: 221 DSYVILDKPAGTTVGGTTDNIEESCATFASRALDLPEPLKTTHQIDNCTEGCVVFARTKE 280

Query: 271 YCSIFHEKIRDKKVKKYYLALVAAPVSCGIITHYMQPAKLAPRIVSEGNNQ 321
           YCS+FH KIR+K+VKK Y AL AAP+  GII+HYM+P  +APR+V+E + Q
Sbjct: 281 YCSVFHTKIRNKEVKKLYRALAAAPLPIGIISHYMRPKNMAPRLVAEDSIQ 326

The following BLAST results are available for this feature:

BLAST of Spo16559.1 vs. NCBI nr
BLAST of Spo16559.1 vs. UniProtKB/TrEMBL
BLAST of Spo16559.1 vs. ExPASy Swiss-Prot
BLAST of Spo16559.1 vs. TAIR (Arabidopsis)

BLAST of Spo16559.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902122870\|gb\|KNA04012.1\|	6.9e-181	99.6	hypothetical protein SOVF_2036...	[more]
gi\|731369863\|ref\|XP_010665548.1\|	4.5e-132	76.0	PREDICTED: RNA pseudouridine s...	[more]
gi\|731369849\|ref\|XP_010665544.1\|	9.8e-111	63.1	PREDICTED: RNA pseudouridine s...	[more]
gi\|902187681\|gb\|KNA10963.1\|	9.1e-109	61.8	hypothetical protein SOVF_1395...	[more]
gi\|778658473\|ref\|XP_011652789.1\|	2.5e-106	75.2	PREDICTED: RNA pseudouridine s...	[more]

BLAST of Spo16559.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9Q9P9_SPIOL	4.8e-181	99.6	Uncharacterized protein OS=Spi...	[more]
A0A0J8E0U7_BETVU	3.1e-132	76.0	Uncharacterized protein OS=Bet...	[more]
A0A0J8B9P8_BETVU	6.8e-111	63.1	Uncharacterized protein OS=Bet...	[more]
A0A0K9QW75_SPIOL	6.4e-109	61.8	Uncharacterized protein (Fragm...	[more]
A0A0A0LX47_CUCSA	1.7e-106	75.2	Uncharacterized protein OS=Cuc...	[more]

BLAST of Spo16559.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 2


Match Name	E-value	Identity	Description
PUS6_ARATH	2.8e-97	60.8	RNA pseudouridine synthase 6, ...	[more]
PUS6_ORYSJ	1.0e-91	62.7	RNA pseudouridine synthase 6, ...	[more]

BLAST of Spo16559.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 1


Match Name	E-value	Identity	Description
AT4G21770.1	1.6e-98	60.8	Pseudouridine synthase family ...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006145	Pseudouridine synthase, RsuA/RluB/C/D/E/F	PFAM	PF00849	PseudoU_synth_2	coord: 212..306 score: 3.8
IPR020103	Pseudouridine synthase, catalytic domain	unknown	SSF55120	Pseudouridine synthase	coord: 197..305 score: 1.6
None	No IPR available	PANTHER	PTHR11079	CYTOSINE DEAMINASE	coord: 160..170 score: 1.7E-31coord: 204..316 score: 1.7
None	No IPR available	PANTHER	PTHR11079:SF79	RNA PSEUDOURIDINE SYNTHASE 6, CHLOROPLASTIC	coord: 160..170 score: 1.7E-31coord: 204..316 score: 1.7

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0001522	pseudouridine synthesis
biological_process	GO:0009451	RNA modification
molecular_function	GO:0009982	pseudouridine synthase activity
molecular_function	GO:0003723	RNA binding