Spo16969.1 (mRNA) | SpinachBase

Overview


Name	Spo16969.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	DNA-directed RNA polymerase II subunit rpb4
Location	chr6 : 22646343 .. 22646759 (-)
Sequence length	417

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCAGGCGAAGAAGAAGAGAACGCATCCGAGTTAAAACTGGGCGAAGATTTCGTGAAGGCAAAGTGTTTGATGAACGGAGAAGTAGCCATGATTCTTGAACACAGGTTAGAGCAATTGCAACAAGTTTCTGATGATCCACTAAATCAAATGCCTCAAGTTTTCGAGAAATCTCTGCAATATGTCAAGCGATTCAGCCGTTACAAGAACCCAGATGCTGTGAGACAGGTTAGGGAAATCCTTAGCCGCCATGAATTAGCCGAATTCGAGCTTTCAGTTCTTGGTAATCTTTGCCCTGAAACTGTTGAGGAAGCCATTGCTATGGTGCCGTCCCTTAAGGATAAAGGAAGAGATCTTGATGATGAAGCAATTGAAAAGATGCTTACTGATCTTGCTCTTGTGAAGAAGTTTGAGTAA

mRNA sequence

ATGTCAGGCGAAGAAGAAGAGAACGCATCCGAGTTAAAACTGGGCGAAGATTTCGTGAAGGCAAAGTGTTTGATGAACGGAGAAGTAGCCATGATTCTTGAACACAGGTTAGAGCAATTGCAACAAGTTTCTGATGATCCACTAAATCAAATGCCTCAAGTTTTCGAGAAATCTCTGCAATATGTCAAGCGATTCAGCCGTTACAAGAACCCAGATGCTGTGAGACAGGTTAGGGAAATCCTTAGCCGCCATGAATTAGCCGAATTCGAGCTTTCAGTTCTTGGTAATCTTTGCCCTGAAACTGTTGAGGAAGCCATTGCTATGGTGCCGTCCCTTAAGGATAAAGGAAGAGATCTTGATGATGAAGCAATTGAAAAGATGCTTACTGATCTTGCTCTTGTGAAGAAGTTTGAGTAA

Coding sequence (CDS)

ATGTCAGGCGAAGAAGAAGAGAACGCATCCGAGTTAAAACTGGGCGAAGATTTCGTGAAGGCAAAGTGTTTGATGAACGGAGAAGTAGCCATGATTCTTGAACACAGGTTAGAGCAATTGCAACAAGTTTCTGATGATCCACTAAATCAAATGCCTCAAGTTTTCGAGAAATCTCTGCAATATGTCAAGCGATTCAGCCGTTACAAGAACCCAGATGCTGTGAGACAGGTTAGGGAAATCCTTAGCCGCCATGAATTAGCCGAATTCGAGCTTTCAGTTCTTGGTAATCTTTGCCCTGAAACTGTTGAGGAAGCCATTGCTATGGTGCCGTCCCTTAAGGATAAAGGAAGAGATCTTGATGATGAAGCAATTGAAAAGATGCTTACTGATCTTGCTCTTGTGAAGAAGTTTGAGTAA

Protein sequence

MSGEEEENASELKLGEDFVKAKCLMNGEVAMILEHRLEQLQQVSDDPLNQMPQVFEKSLQYVKRFSRYKNPDAVRQVREILSRHELAEFELSVLGNLCPETVEEAIAMVPSLKDKGRDLDDEAIEKMLTDLALVKKFE

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo16969	Spo16969	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo16969.1	Spo16969.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo16969.1.CDS.1	Spo16969.1.CDS.1	CDS

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo16969.1.exon.1	Spo16969.1.exon.1	exon

Homology

[Close]

BLAST of Spo16969.1 vs. NCBI nr
Match: gi|902205476|gb|KNA15168.1| (hypothetical protein SOVF_100230 [Spinacia oleracea])

HSP 1 Score: 267.7 bits (683), Expect = 1.100e-68
Identity = 138/138 (100.00%), Postives = 138/138 (100.00%), Query Frame = 1


		  
Query: 1   MSGEEEENASELKLGEDFVKAKCLMNGEVAMILEHRLEQLQQVSDDPLNQMPQVFEKSLQ 60
           MSGEEEENASELKLGEDFVKAKCLMNGEVAMILEHRLEQLQQVSDDPLNQMPQVFEKSLQ
Sbjct: 1   MSGEEEENASELKLGEDFVKAKCLMNGEVAMILEHRLEQLQQVSDDPLNQMPQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREILSRHELAEFELSVLGNLCPETVEEAIAMVPSLKDKGRDLD 120
           YVKRFSRYKNPDAVRQVREILSRHELAEFELSVLGNLCPETVEEAIAMVPSLKDKGRDLD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRHELAEFELSVLGNLCPETVEEAIAMVPSLKDKGRDLD 120

Query: 121 DEAIEKMLTDLALVKKFE 139
           DEAIEKMLTDLALVKKFE
Sbjct: 121 DEAIEKMLTDLALVKKFE 138

[Close]

BLAST of Spo16969.1 vs. NCBI nr
Match: gi|731347082|ref|XP_010684798.1| (PREDICTED: DNA-directed RNA polymerase II subunit 4 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 256.1 bits (653), Expect = 3.400e-65
Identity = 131/138 (94.93%), Postives = 135/138 (97.83%), Query Frame = 1


		  
Query: 1   MSGEEEENASELKLGEDFVKAKCLMNGEVAMILEHRLEQLQQVSDDPLNQMPQVFEKSLQ 60
           MSGEEEENA+ELKLGEDFVKAKCLMNGEVAMILEHRLEQLQQVS+DPLNQMPQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKLGEDFVKAKCLMNGEVAMILEHRLEQLQQVSEDPLNQMPQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREILSRHELAEFELSVLGNLCPETVEEAIAMVPSLKDKGRDLD 120
           YVKRFSRYKN DAVRQVREILSRH+LAEFEL VLGNLCPETVEEAIAMVPSLKDKGRDLD
Sbjct: 61  YVKRFSRYKNQDAVRQVREILSRHQLAEFELGVLGNLCPETVEEAIAMVPSLKDKGRDLD 120

Query: 121 DEAIEKMLTDLALVKKFE 139
           DEAI+KML DLALVKKFE
Sbjct: 121 DEAIDKMLNDLALVKKFE 138

[Close]

BLAST of Spo16969.1 vs. NCBI nr
Match: gi|224083811|ref|XP_002307132.1| (hypothetical protein POPTR_0005s08690g [Populus trichocarpa])

HSP 1 Score: 231.1 bits (588), Expect = 1.200e-57
Identity = 116/138 (84.06%), Postives = 130/138 (94.20%), Query Frame = 1


		  
Query: 1   MSGEEEENASELKLGEDFVKAKCLMNGEVAMILEHRLEQLQQVSDDPLNQMPQVFEKSLQ 60
           MSGEEEENA+ELK+G+DF+KAKCLMN EVA+ILEH+ EQLQQ+SDDP+NQ+ QVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDDFLKAKCLMNCEVALILEHKYEQLQQMSDDPMNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREILSRHELAEFELSVLGNLCPETVEEAIAMVPSLKDKGRDLD 120
           YVKRFSRYKNPDAVRQVREILSR++LAEFEL VLGNLCPETVEEAIAMVPS+K KGR  D
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSIKTKGRAHD 120

Query: 121 DEAIEKMLTDLALVKKFE 139
           DEAIEKML DL+L+KKFE
Sbjct: 121 DEAIEKMLNDLSLIKKFE 138

[Close]

BLAST of Spo16969.1 vs. NCBI nr
Match: gi|802759611|ref|XP_012089384.1| (PREDICTED: DNA-directed RNA polymerase II subunit 4 [Jatropha curcas])

HSP 1 Score: 230.7 bits (587), Expect = 1.500e-57
Identity = 116/138 (84.06%), Postives = 130/138 (94.20%), Query Frame = 1


		  
Query: 1   MSGEEEENASELKLGEDFVKAKCLMNGEVAMILEHRLEQLQQVSDDPLNQMPQVFEKSLQ 60
           MSGEEEENASELK+G++F+KAKCLMN EVA+ILEH+ EQLQQ+SDDP+NQ+ QVFEKSLQ
Sbjct: 1   MSGEEEENASELKIGDEFLKAKCLMNCEVALILEHKYEQLQQMSDDPMNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREILSRHELAEFELSVLGNLCPETVEEAIAMVPSLKDKGRDLD 120
           YVKRFSRYKNPDAVRQVREILSR++LAEFEL VLGNLCPETVEEAIAMVPS+K KGR  D
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSIKTKGRAHD 120

Query: 121 DEAIEKMLTDLALVKKFE 139
           DEAIEKML DL+L+KKFE
Sbjct: 121 DEAIEKMLNDLSLIKKFE 138

[Close]

BLAST of Spo16969.1 vs. NCBI nr
Match: gi|225446712|ref|XP_002282413.1| (PREDICTED: DNA-directed RNA polymerase II subunit 4 [Vitis vinifera])

HSP 1 Score: 229.9 bits (585), Expect = 2.600e-57
Identity = 116/138 (84.06%), Postives = 130/138 (94.20%), Query Frame = 1


		  
Query: 1   MSGEEEENASELKLGEDFVKAKCLMNGEVAMILEHRLEQLQQVSDDPLNQMPQVFEKSLQ 60
           MSGEEEENA+ELK+G++F+KAKCLMN EVA+ILEH+ EQLQQ+SDDPLNQ+ QVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVAVILEHKYEQLQQMSDDPLNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREILSRHELAEFELSVLGNLCPETVEEAIAMVPSLKDKGRDLD 120
           YVKRFSRYKNPDAVRQVREILSR++LAEFEL VLGNLCPETVEEAIAMVPS+K KGR  D
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSIKTKGRAHD 120

Query: 121 DEAIEKMLTDLALVKKFE 139
           DEAIEKML DL+L+KKFE
Sbjct: 121 DEAIEKMLNDLSLIKKFE 138

[Close]

BLAST of Spo16969.1 vs. UniProtKB/TrEMBL
Match: A0A0K9R6N5_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_100230 PE=4 SV=1)

HSP 1 Score: 267.7 bits (683), Expect = 7.900e-69
Identity = 138/138 (100.00%), Postives = 138/138 (100.00%), Query Frame = 1


		  
Query: 1   MSGEEEENASELKLGEDFVKAKCLMNGEVAMILEHRLEQLQQVSDDPLNQMPQVFEKSLQ 60
           MSGEEEENASELKLGEDFVKAKCLMNGEVAMILEHRLEQLQQVSDDPLNQMPQVFEKSLQ
Sbjct: 1   MSGEEEENASELKLGEDFVKAKCLMNGEVAMILEHRLEQLQQVSDDPLNQMPQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREILSRHELAEFELSVLGNLCPETVEEAIAMVPSLKDKGRDLD 120
           YVKRFSRYKNPDAVRQVREILSRHELAEFELSVLGNLCPETVEEAIAMVPSLKDKGRDLD
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRHELAEFELSVLGNLCPETVEEAIAMVPSLKDKGRDLD 120

Query: 121 DEAIEKMLTDLALVKKFE 139
           DEAIEKMLTDLALVKKFE
Sbjct: 121 DEAIEKMLTDLALVKKFE 138

[Close]

BLAST of Spo16969.1 vs. UniProtKB/TrEMBL
Match: A0A0J8C0F0_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_7g166230 PE=4 SV=1)

HSP 1 Score: 256.1 bits (653), Expect = 2.400e-65
Identity = 131/138 (94.93%), Postives = 135/138 (97.83%), Query Frame = 1


		  
Query: 1   MSGEEEENASELKLGEDFVKAKCLMNGEVAMILEHRLEQLQQVSDDPLNQMPQVFEKSLQ 60
           MSGEEEENA+ELKLGEDFVKAKCLMNGEVAMILEHRLEQLQQVS+DPLNQMPQVFEKSLQ
Sbjct: 1   MSGEEEENAAELKLGEDFVKAKCLMNGEVAMILEHRLEQLQQVSEDPLNQMPQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREILSRHELAEFELSVLGNLCPETVEEAIAMVPSLKDKGRDLD 120
           YVKRFSRYKN DAVRQVREILSRH+LAEFEL VLGNLCPETVEEAIAMVPSLKDKGRDLD
Sbjct: 61  YVKRFSRYKNQDAVRQVREILSRHQLAEFELGVLGNLCPETVEEAIAMVPSLKDKGRDLD 120

Query: 121 DEAIEKMLTDLALVKKFE 139
           DEAI+KML DLALVKKFE
Sbjct: 121 DEAIDKMLNDLALVKKFE 138

[Close]

BLAST of Spo16969.1 vs. UniProtKB/TrEMBL
Match: B9H5K4_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0005s08690g PE=4 SV=1)

HSP 1 Score: 231.1 bits (588), Expect = 8.200e-58
Identity = 116/138 (84.06%), Postives = 130/138 (94.20%), Query Frame = 1


		  
Query: 1   MSGEEEENASELKLGEDFVKAKCLMNGEVAMILEHRLEQLQQVSDDPLNQMPQVFEKSLQ 60
           MSGEEEENA+ELK+G+DF+KAKCLMN EVA+ILEH+ EQLQQ+SDDP+NQ+ QVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDDFLKAKCLMNCEVALILEHKYEQLQQMSDDPMNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREILSRHELAEFELSVLGNLCPETVEEAIAMVPSLKDKGRDLD 120
           YVKRFSRYKNPDAVRQVREILSR++LAEFEL VLGNLCPETVEEAIAMVPS+K KGR  D
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSIKTKGRAHD 120

Query: 121 DEAIEKMLTDLALVKKFE 139
           DEAIEKML DL+L+KKFE
Sbjct: 121 DEAIEKMLNDLSLIKKFE 138

[Close]

BLAST of Spo16969.1 vs. UniProtKB/TrEMBL
Match: B9S7P5_RICCO (DNA-directed RNA polymerase II, putative OS=Ricinus communis GN=RCOM_0610380 PE=4 SV=1)

HSP 1 Score: 229.9 bits (585), Expect = 1.800e-57
Identity = 115/138 (83.33%), Postives = 131/138 (94.93%), Query Frame = 1


		  
Query: 1   MSGEEEENASELKLGEDFVKAKCLMNGEVAMILEHRLEQLQQVSDDPLNQMPQVFEKSLQ 60
           MSGEEEENASELK+G++F+KAKCLMN EVA+ILEH+ EQLQQ+S+DP+NQ+ QVFEKSLQ
Sbjct: 1   MSGEEEENASELKIGDEFLKAKCLMNCEVALILEHKYEQLQQMSNDPMNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREILSRHELAEFELSVLGNLCPETVEEAIAMVPSLKDKGRDLD 120
           YVKRFSRYKNPDAVRQVREILSR++LAEFEL VLGNLCPETVEEAIAMVPS+K KGR  D
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSIKTKGRAHD 120

Query: 121 DEAIEKMLTDLALVKKFE 139
           DEAIEKMLTDL+++KKFE
Sbjct: 121 DEAIEKMLTDLSMIKKFE 138

[Close]

BLAST of Spo16969.1 vs. UniProtKB/TrEMBL
Match: A5APH2_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_12s0028g01400 PE=4 SV=1)

HSP 1 Score: 229.9 bits (585), Expect = 1.800e-57
Identity = 116/138 (84.06%), Postives = 130/138 (94.20%), Query Frame = 1


		  
Query: 1   MSGEEEENASELKLGEDFVKAKCLMNGEVAMILEHRLEQLQQVSDDPLNQMPQVFEKSLQ 60
           MSGEEEENA+ELK+G++F+KAKCLMN EVA+ILEH+ EQLQQ+SDDPLNQ+ QVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVAVILEHKYEQLQQMSDDPLNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREILSRHELAEFELSVLGNLCPETVEEAIAMVPSLKDKGRDLD 120
           YVKRFSRYKNPDAVRQVREILSR++LAEFEL VLGNLCPETVEEAIAMVPS+K KGR  D
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRYQLAEFELCVLGNLCPETVEEAIAMVPSIKTKGRAHD 120

Query: 121 DEAIEKMLTDLALVKKFE 139
           DEAIEKML DL+L+KKFE
Sbjct: 121 DEAIEKMLNDLSLIKKFE 138

[Close]

BLAST of Spo16969.1 vs. ExPASy Swiss-Prot
Match: NRPB4_ARATH (DNA-directed RNA polymerase II subunit 4 OS=Arabidopsis thaliana GN=NRPB4 PE=1 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 4.700e-59
Identity = 113/138 (81.88%), Postives = 129/138 (93.48%), Query Frame = 1


		  
Query: 1   MSGEEEENASELKLGEDFVKAKCLMNGEVAMILEHRLEQLQQVSDDPLNQMPQVFEKSLQ 60
           MSGEEEENA+ELK+G++F+KAKCLMN EV++ILEH+ EQLQQ+S+DP+NQ+ QVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKFEQLQQISEDPMNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREILSRHELAEFELSVLGNLCPETVEEAIAMVPSLKDKGRDLD 120
           YVKRFSRYKNPDAVRQVREILSRH+L EFEL VLGNLCPETVEEA+AMVPSLK KGR  D
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRHQLTEFELCVLGNLCPETVEEAVAMVPSLKTKGRAHD 120

Query: 121 DEAIEKMLTDLALVKKFE 139
           DEAIEKML DL+LVK+FE
Sbjct: 121 DEAIEKMLNDLSLVKRFE 138

[Close]

BLAST of Spo16969.1 vs. ExPASy Swiss-Prot
Match: RPB4_HUMAN (DNA-directed RNA polymerase II subunit RPB4 OS=Homo sapiens GN=POLR2D PE=1 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 1.600e-22
Identity = 55/137 (40.15%), Postives = 92/137 (67.15%), Query Frame = 1


		  
Query: 2   SGEEEENASELKLGEDFVKAKCLMNGEVAMILEHRLEQLQQVSDDPLNQMPQVFEKSLQY 61
           +G+ EE+AS+L   ++F  A+ L+N EV M+LEHR +Q +   D+   ++ +VF K+L Y
Sbjct: 10  AGDVEEDASQLIFPKEFETAETLLNSEVHMLLEHRKQQNESAEDE--QELSEVFMKTLNY 69

Query: 62  VKRFSRYKNPDAVRQVREILSRHELAEFELSVLGNLCPETVEEAIAMVPSLKDKGRDLDD 121
             RFSR+KN + +  VR +L + +L +FEL+ L NLCPET EE+ A++PSL+ +    +D
Sbjct: 70  TARFSRFKNRETIASVRSLLLQKKLHKFELACLANLCPETAEESKALIPSLEGR---FED 129

Query: 122 EAIEKMLTDLALVKKFE 139
           E ++++L D+   + F+
Sbjct: 130 EELQQILDDIQTKRSFQ 141

[Close]

BLAST of Spo16969.1 vs. ExPASy Swiss-Prot
Match: RPB4_MOUSE (DNA-directed RNA polymerase II subunit RPB4 OS=Mus musculus GN=Polr2d PE=1 SV=2)

HSP 1 Score: 107.1 bits (266), Expect = 1.600e-22
Identity = 55/137 (40.15%), Postives = 92/137 (67.15%), Query Frame = 1


		  
Query: 2   SGEEEENASELKLGEDFVKAKCLMNGEVAMILEHRLEQLQQVSDDPLNQMPQVFEKSLQY 61
           +G+ EE+AS+L   ++F  A+ L+N EV M+LEHR +Q +   D+   ++ +VF K+L Y
Sbjct: 10  AGDVEEDASQLIFPKEFETAETLLNSEVHMLLEHRKQQNESAEDE--QELSEVFMKTLNY 69

Query: 62  VKRFSRYKNPDAVRQVREILSRHELAEFELSVLGNLCPETVEEAIAMVPSLKDKGRDLDD 121
             RFSR+KN + +  VR +L + +L +FEL+ L NLCPET EE+ A++PSL+ +    +D
Sbjct: 70  TARFSRFKNRETIASVRSLLLQKKLHKFELACLANLCPETAEESKALIPSLEGR---FED 129

Query: 122 EAIEKMLTDLALVKKFE 139
           E ++++L D+   + F+
Sbjct: 130 EELQQILDDIQTKRSFQ 141

[Close]

BLAST of Spo16969.1 vs. ExPASy Swiss-Prot
Match: RPB4_DICDI (DNA-directed RNA polymerase II subunit rpb4 OS=Dictyostelium discoideum GN=polr2d PE=3 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 2.200e-19
Identity = 55/147 (37.41%), Postives = 89/147 (60.54%), Query Frame = 1


		  
Query: 4   EEEENASELKLGEDFVKAKCLMNGEVAMILEHRL-------------EQLQQVSDDPLNQ 63
           +EEE+ + LK   D   AK L+N EVA++LEHR                L  +  + +N 
Sbjct: 12  QEEEDLTTLKFPRDLKDAKFLLNSEVAILLEHRKGISESEGTEFPQNTLLIHLYYNSIND 71

Query: 64  MPQVFEKSLQYVKRFSRYKNPDAVRQVREILSRHELAEFELSVLGNLCPETVEEAIAMVP 123
           + + F K+L Y ++FSRYKN  +++QVR  LS+  L EFE++ L NLCPE  +EA +++P
Sbjct: 72  IIRTFHKTLAYAEKFSRYKNKTSIKQVRTALSKQNLEEFEIASLANLCPEISDEAKSLIP 131

Query: 124 SLKDKGRDLDDEAIEKMLTDLALVKKF 138
           SLK     ++D+ ++ +L +L+ ++KF
Sbjct: 132 SLK----KMEDDELQAILDELSNLRKF 154

[Close]

BLAST of Spo16969.1 vs. ExPASy Swiss-Prot
Match: RPB4_DROME (DNA-directed RNA polymerase II 16 kDa polypeptide OS=Drosophila melanogaster GN=Rpb4 PE=2 SV=5)

HSP 1 Score: 86.7 bits (213), Expect = 2.200e-16
Identity = 44/133 (33.08%), Postives = 81/133 (60.90%), Query Frame = 1


		  
Query: 6   EENASELKLGEDFVKAKCLMNGEVAMILEHRLEQLQQVSDDPLNQMPQVFEKSLQYVKRF 65
           +E+A++L+  ++F  A+ L+  EV M+L+HR  Q +   ++   +  +VF K+  Y   F
Sbjct: 11  DEDAADLQFPKEFENAETLLISEVHMLLDHRKRQNESADEE--QEFSEVFMKTYAYTDSF 70

Query: 66  SRYKNPDAVRQVREILSRHELAEFELSVLGNLCPETVEEAIAMVPSLKDKGRDLDDEAIE 125
            ++KN + +   R +L + +L +FEL+ LGNLCPE  EEA A++PSL+ +    +DE + 
Sbjct: 71  RKFKNKETIMSARSLLMQKKLHKFELAALGNLCPEAPEEAKALIPSLEGR---FEDEELR 130

Query: 126 KMLTDLALVKKFE 139
           ++L D+   +  +
Sbjct: 131 QILDDIGTKRSLQ 138

[Close]

BLAST of Spo16969.1 vs. TAIR (Arabidopsis)
Match: AT5G09920.1 (RNA polymerase II, Rpb4, core protein)

HSP 1 Score: 228.4 bits (581), Expect = 2.700e-60
Identity = 113/138 (81.88%), Postives = 129/138 (93.48%), Query Frame = 1


		  
Query: 1   MSGEEEENASELKLGEDFVKAKCLMNGEVAMILEHRLEQLQQVSDDPLNQMPQVFEKSLQ 60
           MSGEEEENA+ELK+G++F+KAKCLMN EV++ILEH+ EQLQQ+S+DP+NQ+ QVFEKSLQ
Sbjct: 1   MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKFEQLQQISEDPMNQVSQVFEKSLQ 60

Query: 61  YVKRFSRYKNPDAVRQVREILSRHELAEFELSVLGNLCPETVEEAIAMVPSLKDKGRDLD 120
           YVKRFSRYKNPDAVRQVREILSRH+L EFEL VLGNLCPETVEEA+AMVPSLK KGR  D
Sbjct: 61  YVKRFSRYKNPDAVRQVREILSRHQLTEFELCVLGNLCPETVEEAVAMVPSLKTKGRAHD 120

Query: 121 DEAIEKMLTDLALVKKFE 139
           DEAIEKML DL+LVK+FE
Sbjct: 121 DEAIEKMLNDLSLVKRFE 138

[Close]

BLAST of Spo16969.1 vs. TAIR (Arabidopsis)
Match: AT4G15950.1 (RNA polymerase II, Rpb4, core protein)

HSP 1 Score: 73.6 bits (179), Expect = 1.100e-13
Identity = 40/118 (33.90%), Postives = 75/118 (63.56%), Query Frame = 1


		  
Query: 21  AKCLMNGEVAMILEHRLEQLQQVSDDPLNQMPQVFEKSLQYVKRFSRYKNPDAVRQVREI 80
           A+C+M+ E   IL+    QL  +S+DP  ++P  ++++L YV+    Y NP +VR+V E 
Sbjct: 89  AECMMDCEAFQILDGIKGQLVGLSEDPSIKIPVSYDRALAYVESCVHYTNPQSVRKVLEP 148

Query: 81  LSRHELAEFELSVLGNLCPETVEEAIAMVPSLKDKGRDLDDEAIEKMLTDLALVKKFE 139
           L  + +++ E+ V+ N   E+V+E +A +PSLK K +++ ++ ++  L +L+ +KK E
Sbjct: 149 LKTYGISDGEMCVIANASSESVDEVLAFIPSLKTK-KEVINQPLQDALEELSKLKKSE 205

The following BLAST results are available for this feature:

BLAST of Spo16969.1 vs. NCBI nr
BLAST of Spo16969.1 vs. UniProtKB/TrEMBL
BLAST of Spo16969.1 vs. ExPASy Swiss-Prot
BLAST of Spo16969.1 vs. TAIR (Arabidopsis)

BLAST of Spo16969.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902205476\|gb\|KNA15168.1\|	1.1e-68	100.	hypothetical protein SOVF_1002...	[more]
gi\|731347082\|ref\|XP_010684798.1\|	3.4e-65	94.9	PREDICTED: DNA-directed RNA po...	[more]
gi\|224083811\|ref\|XP_002307132.1\|	1.2e-57	84.0	hypothetical protein POPTR_000...	[more]
gi\|802759611\|ref\|XP_012089384.1\|	1.5e-57	84.0	PREDICTED: DNA-directed RNA po...	[more]
gi\|225446712\|ref\|XP_002282413.1\|	2.6e-57	84.0	PREDICTED: DNA-directed RNA po...	[more]

BLAST of Spo16969.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9R6N5_SPIOL	7.9e-69	100.	Uncharacterized protein OS=Spi...	[more]
A0A0J8C0F0_BETVU	2.4e-65	94.9	Uncharacterized protein OS=Bet...	[more]
B9H5K4_POPTR	8.2e-58	84.0	Uncharacterized protein OS=Pop...	[more]
B9S7P5_RICCO	1.8e-57	83.3	DNA-directed RNA polymerase II...	[more]
A5APH2_VITVI	1.8e-57	84.0	Putative uncharacterized prote...	[more]

BLAST of Spo16969.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
NRPB4_ARATH	4.7e-59	81.8	DNA-directed RNA polymerase II...	[more]
RPB4_HUMAN	1.6e-22	40.1	DNA-directed RNA polymerase II...	[more]
RPB4_MOUSE	1.6e-22	40.1	DNA-directed RNA polymerase II...	[more]
RPB4_DICDI	2.2e-19	37.4	DNA-directed RNA polymerase II...	[more]
RPB4_DROME	2.2e-16	33.0	DNA-directed RNA polymerase II...	[more]

BLAST of Spo16969.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 2


Match Name	E-value	Identity	Description
AT5G09920.1	2.7e-60	81.8	RNA polymerase II, Rpb4, core ...	[more]
AT4G15950.1	1.1e-13	33.9	RNA polymerase II, Rpb4, core ...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005574	RNA polymerase II, Rpb4	PFAM	PF03874	RNA_pol_Rpb4	coord: 23..132 score: 2.4
IPR006590	RNA polymerase II, Rpb4, core	SMART	SM00657	rpol4neu2	coord: 16..138 score: 7.2
IPR010997	HRDC-like	unknown	SSF47819	HRDC-like	coord: 3..132 score: 2.18
None	No IPR available	PANTHER	PTHR21297	DNA-DIRECTED RNA POLYMERASE II	coord: 1..138 score: 9.3

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0044237	cellular metabolic process
biological_process	GO:0006144	purine nucleobase metabolic process
biological_process	GO:0006367	transcription initiation from RNA polymerase II promoter
biological_process	GO:0000394	RNA splicing, via endonucleolytic cleavage and ligation
biological_process	GO:0034402	recruitment of 3'-end processing factors to RNA polymerase II holoenzyme complex
biological_process	GO:0006351	transcription, DNA-templated
biological_process	GO:0006206	pyrimidine nucleobase metabolic process
biological_process	GO:0045948	positive regulation of translational initiation
biological_process	GO:0000288	nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay
biological_process	GO:0031990	mRNA export from nucleus in response to heat stress
cellular_component	GO:0005665	DNA-directed RNA polymerase II, core complex
cellular_component	GO:0005730	nucleolus
cellular_component	GO:0000932	P-body
molecular_function	GO:0000166	nucleotide binding
molecular_function	GO:0003899	DNA-directed 5'-3' RNA polymerase activity
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0003697	single-stranded DNA binding
molecular_function	GO:0003727	single-stranded RNA binding
molecular_function	GO:0031369	translation initiation factor binding