Spo18276.1 (mRNA) | SpinachBase

Overview


Name	Spo18276.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	Mitochondrial import inner membrane translocase subunit tim23, putative
Location	chr4 : 122823447 .. 122825769 (-)
Sequence length	747

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTAAATGTTAGTAGGAACCTTATTAAACCTGCTCATGACCTTGCTAATATAGCAAGAAAACATGGGATGGTCGATGTTGTATAGTTTTGTGTGTTTACTTTTGTTGTCAAAAAAAAAAAAAACTATGACCAAAAAAATATTACTTCGCATAAAAGTACATATTTACAACTGCAAGCACATAACTAGGAAGAAAAGCACATGTTCAATTGGAAAAGGAAATCAACACAAAATAGGACAAAATCTAATTACTGGTGATAAACACTACGTCTGCCATTGATTAAGATGCATGACTGTTCTTGGGTGTAAAGAAAACCCATAAAATTCCAATTAATAATAGATAAAATTAACAAAAACTGTCAAAATAAATAAAATAAATTAACAATTAAATTACACATGAAAATAACATCAATACACACTTACTTGAATAAATGAAGATTAACACATACGAAATTTACGAATTTGAGCAACTGTGTCGCCTAATTTGCGATTTTTGGAGATGAAGATGTTCTTTATTTTGGTTTTCCCCTTTTGACGACATCAACGAGGATTCTAATTGATTCTCTAAATCTTTTCCTTTTTCTATTGACTTTTTATTTGGCAGTTTTTTAAATTTAAAAATTCTATCCTAATAACTGCCAAAAGATACCAAAATACCCGGAAAGTAATTTTGTGTTATTAATAATTTTTGTTTGGGTCTATAGATCTGTTGTAGCCGTGACAAATATGATTTCCGTCTAATTCGTCCGATTGTAGTTGAAAGATTGATATCCTCTTCGATCCCGCATAAATCTTTACCAAAGAAACAAGCTGAACTAAACTAAACACACAAAACTTAACTAAAAACAAACCCACCATAAGGGTACTTACTACACCGAAACAGTCAAACCCACCATAGGGTACTTACCACACTGAAACTATGTAATTTTATTGAGATCTAACGCAAAAACACAACAAAATTGAAAGAGAAGGGGCGGAAATAACCAAATTTCCGAAGAAAATTGGCTATTTCCGCCCTAGAAAACCCTAATTTTTGTAAACTCTAAAAAGAGAGAGAAAAGAGGGAGAGAGATATGAGTTAGAAAACCTACGAACGGAGTATGTGATTATAAACACATAACACGGATTGTTTTTTAATTTTAACATTGTTCTTGTTTACTCCCTCCGTCCCTTAATACTCGCACCGTTTCTACTTGACACGCTTGCCAATGCACAACTTTGACCACCAATATCTTTAACTACATATTATAAAAACTTGTAAAAATATTAATATTTTGAAAATATATATTAAGATGAAGCTAATAATATATTATATACTAACATTTATTTTCATATACTAAAAATAAATTAGGGCAAAAGTGAATTATGTGAATAGTGCAAAAAGGCAAAACGGTGCGAGTATTAAGGGACAGAGGGAGTATACCATTGTTCTTTTGTGATACCATTATTTTTAACCAAACGGGCAAACGAAACTTTAATTTTCCGTATACAAATATCCAGTGTATTCATTTAACACGGATTTAATAGAGTTGTTTAAATGTGTATTGCAAGTTGTAAGGCGGTGTTGAGGGTTAAGTAGTCTCCGCATTCTCGAAAAACTTCCGACTCGGACGCCTCTTCTCTCTCTCTCTCCTCCCCTTTCCAACCAAAATCAGAAAACTCCGAGGGTTGCCAAAGAAGAATCTTCCCCATCGGAAATCCCTCTAACCCTAACCCTAACCCTAATCCTAAAATCTGTCAATCGAACCTCGAAAATTTCACAAATATGTCGTTCAATCAACCAAAAGAGGACGAGAATAATGAGAATCGTCGTCTCTACAATCCGTACAAAGACTTAGAAATCCAGGCCCAGAAGCTCTATCAGCTTCCCACCCAACCCGAATTCCTTTTCCATGAAGAATCCGTCCGACAAAGCAGATCTTGGGGTGAGAATCTCACCTACTACACCGGTTGTGGCTATCTGGCCGGAGCTTCCACCGGCGCCGCCAAGGGACTCGTCGACGCTATCCGTACTTCAGAACACGGTGATACCATGAAACTCCGTGTTAATCGTGTCCTGAACTCTTCCGGCCTCACCGGTCGCCAGTGGGGAAACCGCGCTGGAGTCATAGGTCTCATGTATGCTGGTCTCGAGAGTGGGCTCGTTAAGTTGAGGGATACTGATGATGTCATCAATAGTGTGGTTGCTGGGCTTGCAACCGGGGCTCTCTTTAAGGCGGCTTCGGGTCCTCGGGCTGCTGCCATTGCTGGTGCCGCTGGCGGCCTACTTGTTGGATGTACGGTTGGGATGAAGCACGCGCTCAAGAGATACATCCCAGTCTGA

mRNA sequence

ATGTCTCCGCATTCTCGAAAAACTTCCGACTCGGACGCCTCTTCTCTCTCTCTCTCCTCCCCTTTCCAACCAAAATCAGAAAACTCCGAGGGTTGCCAAAGAAGAATCTTCCCCATCGGAAATCCCTCTAACCCTAACCCTAACCCTAATCCTAAAATCTGTCAATCGAACCTCGAAAATTTCACAAATATGTCGTTCAATCAACCAAAAGAGGACGAGAATAATGAGAATCGTCGTCTCTACAATCCGTACAAAGACTTAGAAATCCAGGCCCAGAAGCTCTATCAGCTTCCCACCCAACCCGAATTCCTTTTCCATGAAGAATCCGTCCGACAAAGCAGATCTTGGGGTGAGAATCTCACCTACTACACCGGTTGTGGCTATCTGGCCGGAGCTTCCACCGGCGCCGCCAAGGGACTCGTCGACGCTATCCGTACTTCAGAACACGGTGATACCATGAAACTCCGTGTTAATCGTGTCCTGAACTCTTCCGGCCTCACCGGTCGCCAGTGGGGAAACCGCGCTGGAGTCATAGGTCTCATGTATGCTGGTCTCGAGAGTGGGCTCGTTAAGTTGAGGGATACTGATGATGTCATCAATAGTGTGGTTGCTGGGCTTGCAACCGGGGCTCTCTTTAAGGCGGCTTCGGGTCCTCGGGCTGCTGCCATTGCTGGTGCCGCTGGCGGCCTACTTGTTGGATGTACGGTTGGGATGAAGCACGCGCTCAAGAGATACATCCCAGTCTGA

Coding sequence (CDS)

ATGTCTCCGCATTCTCGAAAAACTTCCGACTCGGACGCCTCTTCTCTCTCTCTCTCCTCCCCTTTCCAACCAAAATCAGAAAACTCCGAGGGTTGCCAAAGAAGAATCTTCCCCATCGGAAATCCCTCTAACCCTAACCCTAACCCTAATCCTAAAATCTGTCAATCGAACCTCGAAAATTTCACAAATATGTCGTTCAATCAACCAAAAGAGGACGAGAATAATGAGAATCGTCGTCTCTACAATCCGTACAAAGACTTAGAAATCCAGGCCCAGAAGCTCTATCAGCTTCCCACCCAACCCGAATTCCTTTTCCATGAAGAATCCGTCCGACAAAGCAGATCTTGGGGTGAGAATCTCACCTACTACACCGGTTGTGGCTATCTGGCCGGAGCTTCCACCGGCGCCGCCAAGGGACTCGTCGACGCTATCCGTACTTCAGAACACGGTGATACCATGAAACTCCGTGTTAATCGTGTCCTGAACTCTTCCGGCCTCACCGGTCGCCAGTGGGGAAACCGCGCTGGAGTCATAGGTCTCATGTATGCTGGTCTCGAGAGTGGGCTCGTTAAGTTGAGGGATACTGATGATGTCATCAATAGTGTGGTTGCTGGGCTTGCAACCGGGGCTCTCTTTAAGGCGGCTTCGGGTCCTCGGGCTGCTGCCATTGCTGGTGCCGCTGGCGGCCTACTTGTTGGATGTACGGTTGGGATGAAGCACGCGCTCAAGAGATACATCCCAGTCTGA

Protein sequence

MSPHSRKTSDSDASSLSLSSPFQPKSENSEGCQRRIFPIGNPSNPNPNPNPKICQSNLENFTNMSFNQPKEDENNENRRLYNPYKDLEIQAQKLYQLPTQPEFLFHEESVRQSRSWGENLTYYTGCGYLAGASTGAAKGLVDAIRTSEHGDTMKLRVNRVLNSSGLTGRQWGNRAGVIGLMYAGLESGLVKLRDTDDVINSVVAGLATGALFKAASGPRAAAIAGAAGGLLVGCTVGMKHALKRYIPV

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo18276	Spo18276	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo18276.1	Spo18276.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo18276.1.CDS.2	Spo18276.1.CDS.2	CDS
Spo18276.1.CDS.1	Spo18276.1.CDS.1	CDS

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo18276.1.exon.2	Spo18276.1.exon.2	exon
Spo18276.1.exon.1	Spo18276.1.exon.1	exon

Homology

[Close]

BLAST of Spo18276.1 vs. NCBI nr
Match: gi|902036307|gb|KNA02639.1| (hypothetical protein SOVF_216800 [Spinacia oleracea])

HSP 1 Score: 362.8 bits (930), Expect = 4.600e-97
Identity = 185/185 (100.00%), Postives = 185/185 (100.00%), Query Frame = 1


		  
Query: 64  MSFNQPKEDENNENRRLYNPYKDLEIQAQKLYQLPTQPEFLFHEESVRQSRSWGENLTYY 123
           MSFNQPKEDENNENRRLYNPYKDLEIQAQKLYQLPTQPEFLFHEESVRQSRSWGENLTYY
Sbjct: 1   MSFNQPKEDENNENRRLYNPYKDLEIQAQKLYQLPTQPEFLFHEESVRQSRSWGENLTYY 60

Query: 124 TGCGYLAGASTGAAKGLVDAIRTSEHGDTMKLRVNRVLNSSGLTGRQWGNRAGVIGLMYA 183
           TGCGYLAGASTGAAKGLVDAIRTSEHGDTMKLRVNRVLNSSGLTGRQWGNRAGVIGLMYA
Sbjct: 61  TGCGYLAGASTGAAKGLVDAIRTSEHGDTMKLRVNRVLNSSGLTGRQWGNRAGVIGLMYA 120

Query: 184 GLESGLVKLRDTDDVINSVVAGLATGALFKAASGPRAAAIAGAAGGLLVGCTVGMKHALK 243
           GLESGLVKLRDTDDVINSVVAGLATGALFKAASGPRAAAIAGAAGGLLVGCTVGMKHALK
Sbjct: 121 GLESGLVKLRDTDDVINSVVAGLATGALFKAASGPRAAAIAGAAGGLLVGCTVGMKHALK 180

Query: 244 RYIPV 249
           RYIPV
Sbjct: 181 RYIPV 185

[Close]

BLAST of Spo18276.1 vs. NCBI nr
Match: gi|731373774|ref|XP_010666746.1| (PREDICTED: mitochondrial import inner membrane translocase subunit TIM23-2-like [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 298.5 bits (763), Expect = 1.100e-77
Identity = 148/185 (80.00%), Postives = 168/185 (90.81%), Query Frame = 1


		  
Query: 64  MSFNQPKEDENNENRRLYNPYKDLEIQAQKLYQLPTQPEFLFHEESVRQSRSWGENLTYY 123
           M+FNQP++D ++E RRLYNPY+DLE   QKLYQLPTQPE+LFHEES+RQ RSWGENLTYY
Sbjct: 1   MAFNQPEKDGDHE-RRLYNPYQDLETPVQKLYQLPTQPEYLFHEESLRQRRSWGENLTYY 60

Query: 124 TGCGYLAGASTGAAKGLVDAIRTSEHGDTMKLRVNRVLNSSGLTGRQWGNRAGVIGLMYA 183
           TGCGYLAGAS+GAAKGL DA+   EHGDT+KLRVNRVLN+SGLTGRQWGNRAGVI LMYA
Sbjct: 61  TGCGYLAGASSGAAKGLFDALHAFEHGDTLKLRVNRVLNTSGLTGRQWGNRAGVIALMYA 120

Query: 184 GLESGLVKLRDTDDVINSVVAGLATGALFKAASGPRAAAIAGAAGGLLVGCTVGMKHALK 243
           G ++G+V  RDTDDV NSV +GLATGA++KAASGPRAAA+AGA GGLLVGCTVG+KHALK
Sbjct: 121 GFDTGIVWWRDTDDVYNSVASGLATGAIYKAASGPRAAAVAGAIGGLLVGCTVGIKHALK 180

Query: 244 RYIPV 249
           RYIPV
Sbjct: 181 RYIPV 184

[Close]

BLAST of Spo18276.1 vs. NCBI nr
Match: gi|731401986|ref|XP_002278010.2| (PREDICTED: mitochondrial import inner membrane translocase subunit TIM23-1 [Vitis vinifera])

HSP 1 Score: 255.8 bits (652), Expect = 8.000e-65
Identity = 128/191 (67.02%), Postives = 159/191 (83.25%), Query Frame = 1


		  
Query: 58  LENFTNMSFNQPKEDENNENRRLYNPYKDLEIQAQKLYQLPTQPEFLFHEESVRQSRSWG 117
           LE+F +M  +Q     +N+NRRLYNPY+DL+I  Q +Y+LPT PEFLF EESV Q RSWG
Sbjct: 49  LESFIHM--DQRTSQNDNDNRRLYNPYQDLQIPIQNIYKLPTSPEFLFQEESVAQRRSWG 108

Query: 118 ENLTYYTGCGYLAGASTGAAKGLVDAIRTSEHGDTMKLRVNRVLNSSGLTGRQWGNRAGV 177
           ENLTYYTG GYL+GA +GAAKGLVD ++ SE GDT+KLR+NR+LN+SG  GR++GNRAGV
Sbjct: 109 ENLTYYTGIGYLSGALSGAAKGLVDGVKASEPGDTVKLRINRILNASGHAGRKFGNRAGV 168

Query: 178 IGLMYAGLESGLVKLRDTDDVINSVVAGLATGALFKAASGPRAAAIAGAAGGLLVGCTVG 237
           IGLMYAGLESG+V +RDTDDV+NSVVAGL TGAL++AA+G R+AA+AGA GG++VG  V 
Sbjct: 169 IGLMYAGLESGIVAVRDTDDVVNSVVAGLGTGALYRAAAGVRSAAVAGAIGGVVVGAAVT 228

Query: 238 MKHALKRYIPV 249
            K  LKRY+P+
Sbjct: 229 AKQVLKRYVPI 237

[Close]

BLAST of Spo18276.1 vs. NCBI nr
Match: gi|720036810|ref|XP_010267477.1| (PREDICTED: mitochondrial import inner membrane translocase subunit TIM23-2-like [Nelumbo nucifera])

HSP 1 Score: 252.3 bits (643), Expect = 8.800e-64
Identity = 122/170 (71.76%), Postives = 144/170 (84.71%), Query Frame = 1


		  
Query: 79  RLYNPYKDLEIQAQKLYQLPTQPEFLFHEESVRQSRSWGENLTYYTGCGYLAGASTGAAK 138
           RLYNPY+DL++  Q LY+LPT PEFLF EE+V Q R+WGENLTYYTGCGYLAGA  GA K
Sbjct: 15  RLYNPYQDLQLPIQTLYKLPTSPEFLFQEEAVAQRRTWGENLTYYTGCGYLAGAIAGAGK 74

Query: 139 GLVDAIRTSEHGDTMKLRVNRVLNSSGLTGRQWGNRAGVIGLMYAGLESGLVKLRDTDDV 198
           G VD ++ SE GDT+KLR+NRVLN+SG +GR+ GNR GVIGL+YAGLESG+V +RDTDDV
Sbjct: 75  GFVDGVKASEEGDTLKLRINRVLNASGHSGRKLGNRVGVIGLLYAGLESGIVAVRDTDDV 134

Query: 199 INSVVAGLATGALFKAASGPRAAAIAGAAGGLLVGCTVGMKHALKRYIPV 249
           INSV+AGL TGALFKAASGPR+AA+AGA GGL+VG  V  K  LKRY+P+
Sbjct: 135 INSVLAGLGTGALFKAASGPRSAAVAGAIGGLMVGAAVAGKQVLKRYVPI 184

[Close]

BLAST of Spo18276.1 vs. NCBI nr
Match: gi|901826567|gb|KMZ75935.1| (Mitochondrial import inner membrane translocase subunit tim23 [Zostera marina])

HSP 1 Score: 251.9 bits (642), Expect = 1.200e-63
Identity = 130/194 (67.01%), Postives = 153/194 (78.87%), Query Frame = 1


		  
Query: 58  LENFTNMSFNQPKEDENNENR---RLYNPYKDLEIQAQKLYQLPTQPEFLFHEESVRQSR 117
           +E+  N   N+  E  NN +R   RLYNPY+DL+I  QK+Y +PT PEFLF EESV Q R
Sbjct: 2   VESGANFYGNKDSETGNNTSRVTGRLYNPYQDLQIPYQKIYNIPTSPEFLFQEESVAQRR 61

Query: 118 SWGENLTYYTGCGYLAGASTGAAKGLVDAIRTSEHGDTMKLRVNRVLNSSGLTGRQWGNR 177
           +WGENLTYYTGCGYL GA +GA KGLV+A+R +E GDT KLRVNRVLNSSG  GR+ GNR
Sbjct: 62  AWGENLTYYTGCGYLVGAVSGAGKGLVEAVRATEPGDTFKLRVNRVLNSSGQAGRRVGNR 121

Query: 178 AGVIGLMYAGLESGLVKLRDTDDVINSVVAGLATGALFKAASGPRAAAIAGAAGGLLVGC 237
            GVIGLMYAG+ES LV +RDTDD++NSVVAGL TG LFKAASGPR+AAIAGA GGL+VG 
Sbjct: 122 CGVIGLMYAGIESALVTVRDTDDLLNSVVAGLGTGVLFKAASGPRSAAIAGAIGGLMVGV 181

Query: 238 TVGMKHALKRYIPV 249
            V  K  +KRY+P+
Sbjct: 182 AVAGKQVVKRYVPI 195

[Close]

BLAST of Spo18276.1 vs. UniProtKB/TrEMBL
Match: A0A0K9Q6J5_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_216800 PE=4 SV=1)

HSP 1 Score: 362.8 bits (930), Expect = 3.200e-97
Identity = 185/185 (100.00%), Postives = 185/185 (100.00%), Query Frame = 1


		  
Query: 64  MSFNQPKEDENNENRRLYNPYKDLEIQAQKLYQLPTQPEFLFHEESVRQSRSWGENLTYY 123
           MSFNQPKEDENNENRRLYNPYKDLEIQAQKLYQLPTQPEFLFHEESVRQSRSWGENLTYY
Sbjct: 1   MSFNQPKEDENNENRRLYNPYKDLEIQAQKLYQLPTQPEFLFHEESVRQSRSWGENLTYY 60

Query: 124 TGCGYLAGASTGAAKGLVDAIRTSEHGDTMKLRVNRVLNSSGLTGRQWGNRAGVIGLMYA 183
           TGCGYLAGASTGAAKGLVDAIRTSEHGDTMKLRVNRVLNSSGLTGRQWGNRAGVIGLMYA
Sbjct: 61  TGCGYLAGASTGAAKGLVDAIRTSEHGDTMKLRVNRVLNSSGLTGRQWGNRAGVIGLMYA 120

Query: 184 GLESGLVKLRDTDDVINSVVAGLATGALFKAASGPRAAAIAGAAGGLLVGCTVGMKHALK 243
           GLESGLVKLRDTDDVINSVVAGLATGALFKAASGPRAAAIAGAAGGLLVGCTVGMKHALK
Sbjct: 121 GLESGLVKLRDTDDVINSVVAGLATGALFKAASGPRAAAIAGAAGGLLVGCTVGMKHALK 180

Query: 244 RYIPV 249
           RYIPV
Sbjct: 181 RYIPV 185

[Close]

BLAST of Spo18276.1 vs. UniProtKB/TrEMBL
Match: A0A0J8DYD3_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_004230 PE=4 SV=1)

HSP 1 Score: 298.5 bits (763), Expect = 7.500e-78
Identity = 148/185 (80.00%), Postives = 168/185 (90.81%), Query Frame = 1


		  
Query: 64  MSFNQPKEDENNENRRLYNPYKDLEIQAQKLYQLPTQPEFLFHEESVRQSRSWGENLTYY 123
           M+FNQP++D ++E RRLYNPY+DLE   QKLYQLPTQPE+LFHEES+RQ RSWGENLTYY
Sbjct: 1   MAFNQPEKDGDHE-RRLYNPYQDLETPVQKLYQLPTQPEYLFHEESLRQRRSWGENLTYY 60

Query: 124 TGCGYLAGASTGAAKGLVDAIRTSEHGDTMKLRVNRVLNSSGLTGRQWGNRAGVIGLMYA 183
           TGCGYLAGAS+GAAKGL DA+   EHGDT+KLRVNRVLN+SGLTGRQWGNRAGVI LMYA
Sbjct: 61  TGCGYLAGASSGAAKGLFDALHAFEHGDTLKLRVNRVLNTSGLTGRQWGNRAGVIALMYA 120

Query: 184 GLESGLVKLRDTDDVINSVVAGLATGALFKAASGPRAAAIAGAAGGLLVGCTVGMKHALK 243
           G ++G+V  RDTDDV NSV +GLATGA++KAASGPRAAA+AGA GGLLVGCTVG+KHALK
Sbjct: 121 GFDTGIVWWRDTDDVYNSVASGLATGAIYKAASGPRAAAVAGAIGGLLVGCTVGIKHALK 180

Query: 244 RYIPV 249
           RYIPV
Sbjct: 181 RYIPV 184

[Close]

BLAST of Spo18276.1 vs. UniProtKB/TrEMBL
Match: F6HXZ8_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_09s0002g01810 PE=4 SV=1)

HSP 1 Score: 253.1 bits (645), Expect = 3.600e-64
Identity = 124/182 (68.13%), Postives = 153/182 (84.07%), Query Frame = 1


		  
Query: 67  NQPKEDENNENRRLYNPYKDLEIQAQKLYQLPTQPEFLFHEESVRQSRSWGENLTYYTGC 126
           +Q     +N+NRRLYNPY+DL+I  Q +Y+LPT PEFLF EESV Q RSWGENLTYYTG 
Sbjct: 2   DQRTSQNDNDNRRLYNPYQDLQIPIQNIYKLPTSPEFLFQEESVAQRRSWGENLTYYTGI 61

Query: 127 GYLAGASTGAAKGLVDAIRTSEHGDTMKLRVNRVLNSSGLTGRQWGNRAGVIGLMYAGLE 186
           GYL+GA +GAAKGLVD ++ SE GDT+KLR+NR+LN+SG  GR++GNRAGVIGLMYAGLE
Sbjct: 62  GYLSGALSGAAKGLVDGVKASEPGDTVKLRINRILNASGHAGRKFGNRAGVIGLMYAGLE 121

Query: 187 SGLVKLRDTDDVINSVVAGLATGALFKAASGPRAAAIAGAAGGLLVGCTVGMKHALKRYI 246
           SG+V +RDTDDV+NSVVAGL TGAL++AA+G R+AA+AGA GG++VG  V  K  LKRY+
Sbjct: 122 SGIVAVRDTDDVVNSVVAGLGTGALYRAAAGVRSAAVAGAIGGVVVGAAVTAKQVLKRYV 181

Query: 247 PV 249
           P+
Sbjct: 182 PI 183

[Close]

BLAST of Spo18276.1 vs. UniProtKB/TrEMBL
Match: A0A0K9Q5U7_ZOSMR (Mitochondrial import inner membrane translocase subunit tim23 OS=Zostera marina GN=ZOSMA_109G00260 PE=4 SV=1)

HSP 1 Score: 251.9 bits (642), Expect = 8.000e-64
Identity = 130/194 (67.01%), Postives = 153/194 (78.87%), Query Frame = 1


		  
Query: 58  LENFTNMSFNQPKEDENNENR---RLYNPYKDLEIQAQKLYQLPTQPEFLFHEESVRQSR 117
           +E+  N   N+  E  NN +R   RLYNPY+DL+I  QK+Y +PT PEFLF EESV Q R
Sbjct: 2   VESGANFYGNKDSETGNNTSRVTGRLYNPYQDLQIPYQKIYNIPTSPEFLFQEESVAQRR 61

Query: 118 SWGENLTYYTGCGYLAGASTGAAKGLVDAIRTSEHGDTMKLRVNRVLNSSGLTGRQWGNR 177
           +WGENLTYYTGCGYL GA +GA KGLV+A+R +E GDT KLRVNRVLNSSG  GR+ GNR
Sbjct: 62  AWGENLTYYTGCGYLVGAVSGAGKGLVEAVRATEPGDTFKLRVNRVLNSSGQAGRRVGNR 121

Query: 178 AGVIGLMYAGLESGLVKLRDTDDVINSVVAGLATGALFKAASGPRAAAIAGAAGGLLVGC 237
            GVIGLMYAG+ES LV +RDTDD++NSVVAGL TG LFKAASGPR+AAIAGA GGL+VG 
Sbjct: 122 CGVIGLMYAGIESALVTVRDTDDLLNSVVAGLGTGVLFKAASGPRSAAIAGAIGGLMVGV 181

Query: 238 TVGMKHALKRYIPV 249
            V  K  +KRY+P+
Sbjct: 182 AVAGKQVVKRYVPI 195

[Close]

BLAST of Spo18276.1 vs. UniProtKB/TrEMBL
Match: A0A118JVF2_CYNCS (Mitochondrial inner membrane translocase subunit Tim17/Tim22/Tim23/peroxisomal protein PMP24 OS=Cynara cardunculus var. scolymus GN=Ccrd_005024 PE=4 SV=1)

HSP 1 Score: 250.0 bits (637), Expect = 3.000e-63
Identity = 124/184 (67.39%), Postives = 149/184 (80.98%), Query Frame = 1


		  
Query: 65  SFNQPKEDENNENRRLYNPYKDLEIQAQKLYQLPTQPEFLFHEESVRQSRSWGENLTYYT 124
           S N  + D++  NRRLYNPY+DL +  Q LY+LPT PE+LF EESV Q RSWGENLTYYT
Sbjct: 7   SSNHNETDDDRHNRRLYNPYQDLGVPVQTLYKLPTSPEYLFQEESVAQRRSWGENLTYYT 66

Query: 125 GCGYLAGASTGAAKGLVDAIRTSEHGDTMKLRVNRVLNSSGLTGRQWGNRAGVIGLMYAG 184
           G GYL GA  GA KGLV+ ++ SE GDTMKLRVNR+LN+SG  GR+ GNRAGVIGL+YAG
Sbjct: 67  GIGYLGGAVVGAGKGLVEGVKASEAGDTMKLRVNRILNASGHAGRKIGNRAGVIGLLYAG 126

Query: 185 LESGLVKLRDTDDVINSVVAGLATGALFKAASGPRAAAIAGAAGGLLVGCTVGMKHALKR 244
           +ESG+V +RDTDD+INSVVAGL TGAL+KAA+GPR+AA+AGA GG+ VG  V  K  LKR
Sbjct: 127 MESGMVAVRDTDDIINSVVAGLGTGALYKAAAGPRSAAVAGAIGGIAVGLAVTGKQVLKR 186

Query: 245 YIPV 249
           Y+P+
Sbjct: 187 YVPI 190

[Close]

BLAST of Spo18276.1 vs. ExPASy Swiss-Prot
Match: TI231_ARATH (Mitochondrial import inner membrane translocase subunit TIM23-1 OS=Arabidopsis thaliana GN=TIM23-1 PE=2 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 1.700e-54
Identity = 106/184 (57.61%), Postives = 141/184 (76.63%), Query Frame = 1


		  
Query: 64  MSFNQPKEDENNENRRLYNPYKDLE--IQAQKLYQLPTQPEFLFHEESVRQSRSWGENLT 123
           M+ N+  + E++EN RLY+PY++ +  I++Q LY+LPT PEFLF EES++Q RSWGENLT
Sbjct: 1   MAINRSSDHESDENTRLYHPYQNYQVPIKSQYLYKLPTSPEFLFTEESLKQRRSWGENLT 60

Query: 124 YYTGCGYLAGASTGAAKGLVDAIRTSEHGDTMKLRVNRVLNSSGLTGRQWGNRAGVIGLM 183
           +YTG GYLAG+  GA+ G+   I++ E+GDT KL++NR+LNSSG  GR WGNR G++GL+
Sbjct: 61  FYTGTGYLAGSVAGASAGIFSGIKSFENGDTTKLKINRILNSSGQAGRTWGNRVGIVGLI 120

Query: 184 YAGLESGLVKLRDTDDVINSVVAGLATGALFKAASGPRAAAIAGAAGGLLVGCTVGMKHA 243
           YAG+ESG+V + D DDV  SVVAGL TGA+F+AA G R+AA+AGA GG+  G  V  K  
Sbjct: 121 YAGIESGVVAVTDKDDVWTSVVAGLGTGAVFRAARGVRSAAVAGAFGGIAAGAVVAGKQV 180

Query: 244 LKRY 246
            KRY
Sbjct: 181 FKRY 184

[Close]

BLAST of Spo18276.1 vs. ExPASy Swiss-Prot
Match: TI232_ARATH (Mitochondrial import inner membrane translocase subunit TIM23-2 OS=Arabidopsis thaliana GN=TIM23-2 PE=1 SV=1)

HSP 1 Score: 212.2 bits (539), Expect = 6.300e-54
Identity = 106/188 (56.38%), Postives = 140/188 (74.47%), Query Frame = 1


		  
Query: 64  MSFNQPKEDENNENRRLYNPYKDLEI---QAQKLYQLPTQPEFLFHEESVRQSRSWGENL 123
           M+ N   +  ++EN RLYNPY++ E+   ++Q LY+LPT PEFLF EE++RQ RSWGENL
Sbjct: 1   MAANNRSDHGSDENTRLYNPYQNYEVPINKSQYLYKLPTSPEFLFTEEALRQRRSWGENL 60

Query: 124 TYYTGCGYLAGASTGAAKGLVDAIRTSEHGDTMKLRVNRVLNSSGLTGRQWGNRAGVIGL 183
           T+YTG  YL G+  GA+ G++  +++ E GDT KL++NR+LNSSG TGR WGNR G+IGL
Sbjct: 61  TFYTGTAYLGGSVAGASVGVITGVKSFESGDTTKLKINRILNSSGQTGRTWGNRIGIIGL 120

Query: 184 MYAGLESGLVKLRDTDDVINSVVAGLATGALFKAASGPRAAAIAGAAGGLLVGCTVGMKH 243
           +YAG+ESG+V   D DDV  SVVAGL TGA+ +AA G R+AA+AGA GGL  G  V  K 
Sbjct: 121 VYAGIESGIVAATDRDDVWTSVVAGLGTGAVCRAARGVRSAAVAGALGGLAAGAVVAGKQ 180

Query: 244 ALKRYIPV 249
            +KRY+P+
Sbjct: 181 IVKRYVPI 188

[Close]

BLAST of Spo18276.1 vs. ExPASy Swiss-Prot
Match: TI233_ARATH (Mitochondrial import inner membrane translocase subunit TIM23-3 OS=Arabidopsis thaliana GN=TIM23-3 PE=2 SV=1)

HSP 1 Score: 176.0 bits (445), Expect = 5.000e-43
Identity = 85/183 (46.45%), Postives = 126/183 (68.85%), Query Frame = 1


		  
Query: 67  NQPKEDENNENRRLYNPYKDLEIQAQKLYQLPTQPEFLFHEESVRQSRSWGENLTYYTGC 126
           N     +  +  R YNPY+ + +  +KLY+LPT PEFLF EE+ ++  +WGENLT++TG 
Sbjct: 6   NHSTGHQQQQKYRQYNPYQQVNLPYRKLYELPTSPEFLFEEEATKKRLTWGENLTFFTGW 65

Query: 127 GYLAGASTGAAKGLVDAIRTSEHGDTMKLRVNRVLNSSGLTGRQWGNRAGVIGLMYAGLE 186
           GY  G+  GA KG +  +R +E G+++K+R NR+LNS GL  R+ GN  G +GLM+A +E
Sbjct: 66  GYCTGSVLGAFKGTIAGMRAAERGESLKIRTNRILNSGGLVARRGGNCLGSVGLMFAAME 125

Query: 187 SGLVKLRDTDD-VINSVVAGLATGALFKAASGPRAAAIAGAAGGLLVGCTVGMKHALKRY 246
           SG+  +RD DD  + +V+AGLATG L++AASGPR+A +AGA GG+     V  +  +KR+
Sbjct: 126 SGVTYMRDGDDGSLTTVIAGLATGVLYRAASGPRSAVVAGAVGGVAALAAVAGRRIVKRF 185

Query: 247 IPV 249
           +P+
Sbjct: 186 VPI 188

[Close]

BLAST of Spo18276.1 vs. ExPASy Swiss-Prot
Match: TIM23_YEAST (Mitochondrial import inner membrane translocase subunit TIM23 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TIM23 PE=1 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 1.900e-10
Identity = 45/126 (35.71%), Postives = 62/126 (49.21%), Query Frame = 1


		  
Query: 113 SRSWGENLTYYTGCGYLAGASTGAAKGLVDAIRTSEHGDTMKLRVNRVLNSSGLTGRQWG 172
           SR W ++L Y TG  YL G   G   G++  ++        KL++N VLN     G   G
Sbjct: 90  SRGWTDDLCYGTGAVYLLGLGIGGFSGMMQGLQNIPPNSPGKLQLNTVLNHITKRGPFLG 149

Query: 173 NRAGVIGLMYAGLESGLVKLRDTDDVINSVVAGLATGALFKAASG--PRAAAIAGAAGGL 232
           N AG++ L Y  + S +  LR   D   S+ AG  TGALFK++ G  P   + A  A   
Sbjct: 150 NNAGILALSYNIINSTIDALRGKHDTAGSIGAGALTGALFKSSKGLKPMGYSSAMVAAAC 209

Query: 233 LVGCTV 237
            V C+V
Sbjct: 210 AVWCSV 215

[Close]

BLAST of Spo18276.1 vs. TAIR (Arabidopsis)
Match: AT1G17530.1 (translocase of inner mitochondrial membrane 23)

HSP 1 Score: 214.2 bits (544), Expect = 9.400e-56
Identity = 106/184 (57.61%), Postives = 141/184 (76.63%), Query Frame = 1


		  
Query: 64  MSFNQPKEDENNENRRLYNPYKDLE--IQAQKLYQLPTQPEFLFHEESVRQSRSWGENLT 123
           M+ N+  + E++EN RLY+PY++ +  I++Q LY+LPT PEFLF EES++Q RSWGENLT
Sbjct: 1   MAINRSSDHESDENTRLYHPYQNYQVPIKSQYLYKLPTSPEFLFTEESLKQRRSWGENLT 60

Query: 124 YYTGCGYLAGASTGAAKGLVDAIRTSEHGDTMKLRVNRVLNSSGLTGRQWGNRAGVIGLM 183
           +YTG GYLAG+  GA+ G+   I++ E+GDT KL++NR+LNSSG  GR WGNR G++GL+
Sbjct: 61  FYTGTGYLAGSVAGASAGIFSGIKSFENGDTTKLKINRILNSSGQAGRTWGNRVGIVGLI 120

Query: 184 YAGLESGLVKLRDTDDVINSVVAGLATGALFKAASGPRAAAIAGAAGGLLVGCTVGMKHA 243
           YAG+ESG+V + D DDV  SVVAGL TGA+F+AA G R+AA+AGA GG+  G  V  K  
Sbjct: 121 YAGIESGVVAVTDKDDVWTSVVAGLGTGAVFRAARGVRSAAVAGAFGGIAAGAVVAGKQV 180

Query: 244 LKRY 246
            KRY
Sbjct: 181 FKRY 184

[Close]

BLAST of Spo18276.1 vs. TAIR (Arabidopsis)
Match: AT1G72750.1 (translocase inner membrane subunit 23-2)

HSP 1 Score: 212.2 bits (539), Expect = 3.600e-55
Identity = 106/188 (56.38%), Postives = 140/188 (74.47%), Query Frame = 1


		  
Query: 64  MSFNQPKEDENNENRRLYNPYKDLEI---QAQKLYQLPTQPEFLFHEESVRQSRSWGENL 123
           M+ N   +  ++EN RLYNPY++ E+   ++Q LY+LPT PEFLF EE++RQ RSWGENL
Sbjct: 1   MAANNRSDHGSDENTRLYNPYQNYEVPINKSQYLYKLPTSPEFLFTEEALRQRRSWGENL 60

Query: 124 TYYTGCGYLAGASTGAAKGLVDAIRTSEHGDTMKLRVNRVLNSSGLTGRQWGNRAGVIGL 183
           T+YTG  YL G+  GA+ G++  +++ E GDT KL++NR+LNSSG TGR WGNR G+IGL
Sbjct: 61  TFYTGTAYLGGSVAGASVGVITGVKSFESGDTTKLKINRILNSSGQTGRTWGNRIGIIGL 120

Query: 184 MYAGLESGLVKLRDTDDVINSVVAGLATGALFKAASGPRAAAIAGAAGGLLVGCTVGMKH 243
           +YAG+ESG+V   D DDV  SVVAGL TGA+ +AA G R+AA+AGA GGL  G  V  K 
Sbjct: 121 VYAGIESGIVAATDRDDVWTSVVAGLGTGAVCRAARGVRSAAVAGALGGLAAGAVVAGKQ 180

Query: 244 ALKRYIPV 249
            +KRY+P+
Sbjct: 181 IVKRYVPI 188

[Close]

BLAST of Spo18276.1 vs. TAIR (Arabidopsis)
Match: AT3G04800.1 (translocase inner membrane subunit 23-3)

HSP 1 Score: 176.0 bits (445), Expect = 2.800e-44
Identity = 85/183 (46.45%), Postives = 126/183 (68.85%), Query Frame = 1


		  
Query: 67  NQPKEDENNENRRLYNPYKDLEIQAQKLYQLPTQPEFLFHEESVRQSRSWGENLTYYTGC 126
           N     +  +  R YNPY+ + +  +KLY+LPT PEFLF EE+ ++  +WGENLT++TG 
Sbjct: 6   NHSTGHQQQQKYRQYNPYQQVNLPYRKLYELPTSPEFLFEEEATKKRLTWGENLTFFTGW 65

Query: 127 GYLAGASTGAAKGLVDAIRTSEHGDTMKLRVNRVLNSSGLTGRQWGNRAGVIGLMYAGLE 186
           GY  G+  GA KG +  +R +E G+++K+R NR+LNS GL  R+ GN  G +GLM+A +E
Sbjct: 66  GYCTGSVLGAFKGTIAGMRAAERGESLKIRTNRILNSGGLVARRGGNCLGSVGLMFAAME 125

Query: 187 SGLVKLRDTDD-VINSVVAGLATGALFKAASGPRAAAIAGAAGGLLVGCTVGMKHALKRY 246
           SG+  +RD DD  + +V+AGLATG L++AASGPR+A +AGA GG+     V  +  +KR+
Sbjct: 126 SGVTYMRDGDDGSLTTVIAGLATGVLYRAASGPRSAVVAGAVGGVAALAAVAGRRIVKRF 185

Query: 247 IPV 249
           +P+
Sbjct: 186 VPI 188

The following BLAST results are available for this feature:

BLAST of Spo18276.1 vs. NCBI nr
BLAST of Spo18276.1 vs. UniProtKB/TrEMBL
BLAST of Spo18276.1 vs. ExPASy Swiss-Prot
BLAST of Spo18276.1 vs. TAIR (Arabidopsis)

BLAST of Spo18276.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902036307\|gb\|KNA02639.1\|	4.6e-97	100.	hypothetical protein SOVF_2168...	[more]
gi\|731373774\|ref\|XP_010666746.1\|	1.1e-77	80.0	PREDICTED: mitochondrial impor...	[more]
gi\|731401986\|ref\|XP_002278010.2\|	8.0e-65	67.0	PREDICTED: mitochondrial impor...	[more]
gi\|720036810\|ref\|XP_010267477.1\|	8.8e-64	71.7	PREDICTED: mitochondrial impor...	[more]
gi\|901826567\|gb\|KMZ75935.1\|	1.2e-63	67.0	Mitochondrial import inner mem...	[more]

BLAST of Spo18276.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9Q6J5_SPIOL	3.2e-97	100.	Uncharacterized protein OS=Spi...	[more]
A0A0J8DYD3_BETVU	7.5e-78	80.0	Uncharacterized protein OS=Bet...	[more]
F6HXZ8_VITVI	3.6e-64	68.1	Putative uncharacterized prote...	[more]
A0A0K9Q5U7_ZOSMR	8.0e-64	67.0	Mitochondrial import inner mem...	[more]
A0A118JVF2_CYNCS	3.0e-63	67.3	Mitochondrial inner membrane t...	[more]

BLAST of Spo18276.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 4


Match Name	E-value	Identity	Description
TI231_ARATH	1.7e-54	57.6	Mitochondrial import inner mem...	[more]
TI232_ARATH	6.3e-54	56.3	Mitochondrial import inner mem...	[more]
TI233_ARATH	5.0e-43	46.4	Mitochondrial import inner mem...	[more]
TIM23_YEAST	1.9e-10	35.7	Mitochondrial import inner mem...	[more]

BLAST of Spo18276.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 3


Match Name	E-value	Identity	Description
AT1G17530.1	9.4e-56	57.6	translocase of inner mitochond...	[more]
AT1G72750.1	3.6e-55	56.3	translocase inner membrane sub...	[more]
AT3G04800.1	2.8e-44	46.4	translocase inner membrane sub...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003397	Mitochondrial inner membrane translocase subunit Tim17/Tim22/Tim23/peroxisomal protein PMP24	PFAM	PF02466	Tim17	coord: 121..231 score: 2.1
None	No IPR available	PANTHER	PTHR15371	TIM23	coord: 52..248 score: 2.8
None	No IPR available	PANTHER	PTHR15371:SF7	SUBFAMILY NOT NAMED	coord: 52..248 score: 2.8

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane