Spo19206.1 (mRNA) | SpinachBase

Overview


Name	Spo19206.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	rRNA intron-encoded homing endonuclease
Location	SpoScf_30023 : 125 .. 1059 (+)
Sequence length	417

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATAACTCGACGGATCGCACGGCCTTTGCGCCGGCGACGCATCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGTGGCCTACCATGGTGGTGACGGGTGACGGAGAATTAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCAATCCTGACACGGGGAGGTAGTGACAATAAATAACAATACCGGGCTCATTGAGTCTGGTAATTGGAATGAGTACAATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGGACCTTGGGGTGGTACGACCGGTCCGCCTTTTGGTGTGCACCGGCCGTCTCGCCTCTTTCGCCGGTGATGCGCTCCTGGCCTTAATTGGCCGGGTCGTGCCTCCGGCACTGTTACTTTGAAGAAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGTATACATTAGCATGGGATAACATTATAGGATTCCGGTCCTATTGTGTTGGCCTTCGGGATCGGAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGAACAACTGCGAAAGCATTTGCCAAGGATGTTTTCATTAATCAAGAACGAAAGTTGGGGGCTCGAAGACGATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCGGCGGATGTTACTTTTAGGACGCCGCCGGCACCTTATGAGAAATCAAAGTTTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAA

mRNA sequence

ATGATAACTCGACGGATCGCACGGCCTTTGCGCCGGCGACGCATCATTCAAATTTCTGCCCTATCAACTTTCGATGTTAAAAAGCTCGTAGTTGGACCTTGGGGTGGTACGACCGGTCCGCCTTTTGGTGTGCACCGGCCGTCTCGCCTCTTTCGCCGACGAACAACTGCGAAAGCATTTGCCAAGGATGTTTTCATTAATCAAGAACGAAAGTTGGGGGCTCGAAGACGATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCGGCGGATGTTACTTTTAGGACGCCGCCGGCACCTTATGAGAAATCAAAGTTTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAA

Coding sequence (CDS)

ATGATAACTCGACGGATCGCACGGCCTTTGCGCCGGCGACGCATCATTCAAATTTCTGCCCTATCAACTTTCGATGTTAAAAAGCTCGTAGTTGGACCTTGGGGTGGTACGACCGGTCCGCCTTTTGGTGTGCACCGGCCGTCTCGCCTCTTTCGCCGACGAACAACTGCGAAAGCATTTGCCAAGGATGTTTTCATTAATCAAGAACGAAAGTTGGGGGCTCGAAGACGATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCGGCGGATGTTACTTTTAGGACGCCGCCGGCACCTTATGAGAAATCAAAGTTTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAA

Protein sequence

MITRRIARPLRRRRIIQISALSTFDVKKLVVGPWGGTTGPPFGVHRPSRLFRRRTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEKSKFLGSGGSMVARLKLKGIDGRAPPGVEPAA

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo19206	Spo19206	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo19206.1	Spo19206.1-protein	polypeptide

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo19206.1.exon.1	Spo19206.1.exon.1	exon
Spo19206.1.exon.2	Spo19206.1.exon.2	exon
Spo19206.1.exon.3	Spo19206.1.exon.3	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo19206.1.CDS.1	Spo19206.1.CDS.1	CDS
Spo19206.1.CDS.2	Spo19206.1.CDS.2	CDS
Spo19206.1.CDS.3	Spo19206.1.CDS.3	CDS

Homology

[Close]

BLAST of Spo19206.1 vs. NCBI nr
Match: gi|922327215|ref|XP_013443000.1| (rRNA intron-encoded homing endonuclease, putative [Medicago truncatula])

HSP 1 Score: 172.6 bits (436), Expect = 4.900e-40
Identity = 100/147 (68.03%), Postives = 104/147 (70.75%), Query Frame = 1


		  
Query: 25  DVKKLVVGPWGGTTGPP-----------------------------FGV----HRPSRLF 84
           +VKKLVVGPW G+ GPP                             FG+    +R SR  
Sbjct: 195 NVKKLVVGPWVGSIGPPMVCTGWLVPSAGDALLALIGRDSDPIVLAFGIGVMINRDSR-G 254

Query: 85  RRRTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEKSKFL 139
             RTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADV FRTP APYEKSK L
Sbjct: 255 HSRTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVAFRTPLAPYEKSKSL 314

[Close]

BLAST of Spo19206.1 vs. NCBI nr
Match: gi|694380941|ref|XP_009366572.1| (PREDICTED: uncharacterized protein LOC103956333 [Pyrus x bretschneideri])

HSP 1 Score: 167.9 bits (424), Expect = 1.200e-38
Identity = 99/142 (69.72%), Postives = 102/142 (71.83%), Query Frame = 1


		  
Query: 5   RIARPLRRR---------RIIQISALSTFDVKKLVVGPWGGTTGPPFGVHRPSRLFRRRT 64
           R ARP  RR         R      L   +  K   GP     G    ++R SR    RT
Sbjct: 231 RSARPFCRRYAPGLNWPGRASGAVTLKKLECSKQDFGPILLAFGIGVMINRDSR---GRT 290

Query: 65  TAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEKSKFLGSGG 124
           TAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEKSKFLGSGG
Sbjct: 291 TAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEKSKFLGSGG 350

Query: 125 SMVARLKLKGIDGRAPPGVEPA 138
           SMVARLKLKGIDGRAPPGVEPA
Sbjct: 351 SMVARLKLKGIDGRAPPGVEPA 369

[Close]

BLAST of Spo19206.1 vs. NCBI nr
Match: gi|1009176457|ref|XP_015869441.1| (PREDICTED: uncharacterized protein LOC107406762 [Ziziphus jujuba])

HSP 1 Score: 167.2 bits (422), Expect = 2.100e-38
Identity = 84/86 (97.67%), Postives = 85/86 (98.84%), Query Frame = 1


		  
Query: 53  RRTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEKSKFLG 112
           RRTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADV +RTPPAPYEKSKFLG
Sbjct: 602 RRTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVAYRTPPAPYEKSKFLG 661

Query: 113 SGGSMVARLKLKGIDGRAPPGVEPAA 139
           SGGSMVARLKLKGIDGRAPPGVEPAA
Sbjct: 662 SGGSMVARLKLKGIDGRAPPGVEPAA 687

[Close]

BLAST of Spo19206.1 vs. NCBI nr
Match: gi|703137890|ref|XP_010106597.1| (hypothetical protein L484_002552 [Morus notabilis])

HSP 1 Score: 164.5 bits (415), Expect = 1.300e-37
Identity = 84/86 (97.67%), Postives = 84/86 (97.67%), Query Frame = 1


		  
Query: 53  RRTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEKSKFLG 112
           RRTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADV FRTP APYEKSKFLG
Sbjct: 107 RRTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVAFRTPLAPYEKSKFLG 166

Query: 113 SGGSMVARLKLKGIDGRAPPGVEPAA 139
           SGGSMVARLKLKGIDGRAPPGVEPAA
Sbjct: 167 SGGSMVARLKLKGIDGRAPPGVEPAA 192

[Close]

BLAST of Spo19206.1 vs. NCBI nr
Match: gi|686983426|gb|AIQ78384.1| (rRNA intron-encoded homing endonuclease, partial [Cenchrus americanus])

HSP 1 Score: 164.1 bits (414), Expect = 1.800e-37
Identity = 83/86 (96.51%), Postives = 84/86 (97.67%), Query Frame = 1


		  
Query: 53  RRTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEKSKFLG 112
           RRTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADV +RTPPAPYEKSK LG
Sbjct: 2   RRTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVAYRTPPAPYEKSKSLG 61

Query: 113 SGGSMVARLKLKGIDGRAPPGVEPAA 139
           SGGSMVARLKLKGIDGRAPPGVEPAA
Sbjct: 62  SGGSMVARLKLKGIDGRAPPGVEPAA 87

[Close]

BLAST of Spo19206.1 vs. UniProtKB/TrEMBL
Match: A0A072TTZ2_MEDTR (Uncharacterized protein OS=Medicago truncatula GN=MTR_0052s0110 PE=4 SV=1)

HSP 1 Score: 172.6 bits (436), Expect = 3.400e-40
Identity = 100/147 (68.03%), Postives = 104/147 (70.75%), Query Frame = 1


		  
Query: 25  DVKKLVVGPWGGTTGPP-----------------------------FGV----HRPSRLF 84
           +VKKLVVGPW G+ GPP                             FG+    +R SR  
Sbjct: 195 NVKKLVVGPWVGSIGPPMVCTGWLVPSAGDALLALIGRDSDPIVLAFGIGVMINRDSR-G 254

Query: 85  RRRTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEKSKFL 139
             RTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADV FRTP APYEKSK L
Sbjct: 255 HSRTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVAFRTPLAPYEKSKSL 314

[Close]

BLAST of Spo19206.1 vs. UniProtKB/TrEMBL
Match: M1DUP9_SOLTU (Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG400044276 PE=4 SV=1)

HSP 1 Score: 168.3 bits (425), Expect = 6.500e-39
Identity = 85/86 (98.84%), Postives = 85/86 (98.84%), Query Frame = 1


		  
Query: 53  RRTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEKSKFLG 112
           RRTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADV FRTPPAPYEKSKFLG
Sbjct: 100 RRTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVAFRTPPAPYEKSKFLG 159

Query: 113 SGGSMVARLKLKGIDGRAPPGVEPAA 139
           SGGSMVARLKLKGIDGRAPPGVEPAA
Sbjct: 160 SGGSMVARLKLKGIDGRAPPGVEPAA 185

[Close]

BLAST of Spo19206.1 vs. UniProtKB/TrEMBL
Match: M1D925_SOLTU (Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG400034813 PE=4 SV=1)

HSP 1 Score: 168.3 bits (425), Expect = 6.500e-39
Identity = 85/86 (98.84%), Postives = 85/86 (98.84%), Query Frame = 1


		  
Query: 53  RRTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEKSKFLG 112
           RRTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADV FRTPPAPYEKSKFLG
Sbjct: 236 RRTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVAFRTPPAPYEKSKFLG 295

Query: 113 SGGSMVARLKLKGIDGRAPPGVEPAA 139
           SGGSMVARLKLKGIDGRAPPGVEPAA
Sbjct: 296 SGGSMVARLKLKGIDGRAPPGVEPAA 321

[Close]

BLAST of Spo19206.1 vs. UniProtKB/TrEMBL
Match: M1DMU8_SOLTU (Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG400041108 PE=4 SV=1)

HSP 1 Score: 168.3 bits (425), Expect = 6.500e-39
Identity = 86/92 (93.48%), Postives = 87/92 (94.57%), Query Frame = 1


		  
Query: 47  PSRLFRRRTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYE 106
           P R +  RTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADV FRTPPAPYE
Sbjct: 48  PHRPWLVRTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVAFRTPPAPYE 107

Query: 107 KSKFLGSGGSMVARLKLKGIDGRAPPGVEPAA 139
           KSKFLGSGGSMVARLKLKGIDGRAPPGVEPAA
Sbjct: 108 KSKFLGSGGSMVARLKLKGIDGRAPPGVEPAA 139

[Close]

BLAST of Spo19206.1 vs. UniProtKB/TrEMBL
Match: W9SJ11_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_002552 PE=4 SV=1)

HSP 1 Score: 164.5 bits (415), Expect = 9.400e-38
Identity = 84/86 (97.67%), Postives = 84/86 (97.67%), Query Frame = 1


		  
Query: 53  RRTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEKSKFLG 112
           RRTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADV FRTP APYEKSKFLG
Sbjct: 107 RRTTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVAFRTPLAPYEKSKFLG 166

Query: 113 SGGSMVARLKLKGIDGRAPPGVEPAA 139
           SGGSMVARLKLKGIDGRAPPGVEPAA
Sbjct: 167 SGGSMVARLKLKGIDGRAPPGVEPAA 192

The following BLAST results are available for this feature:

BLAST of Spo19206.1 vs. NCBI nr
BLAST of Spo19206.1 vs. UniProtKB/TrEMBL
BLAST of Spo19206.1 vs. ExPASy Swiss-Prot
BLAST of Spo19206.1 vs. TAIR (Arabidopsis)

BLAST of Spo19206.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|922327215\|ref\|XP_013443000.1\|	4.9e-40	68.0	rRNA intron-encoded homing end...	[more]
gi\|694380941\|ref\|XP_009366572.1\|	1.2e-38	69.7	PREDICTED: uncharacterized pro...	[more]
gi\|1009176457\|ref\|XP_015869441.1\|	2.1e-38	97.6	PREDICTED: uncharacterized pro...	[more]
gi\|703137890\|ref\|XP_010106597.1\|	1.3e-37	97.6	hypothetical protein L484_0025...	[more]
gi\|686983426\|gb\|AIQ78384.1\|	1.8e-37	96.5	rRNA intron-encoded homing end...	[more]

BLAST of Spo19206.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A072TTZ2_MEDTR	3.4e-40	68.0	Uncharacterized protein OS=Med...	[more]
M1DUP9_SOLTU	6.5e-39	98.8	Uncharacterized protein OS=Sol...	[more]
M1D925_SOLTU	6.5e-39	98.8	Uncharacterized protein OS=Sol...	[more]
M1DMU8_SOLTU	6.5e-39	93.4	Uncharacterized protein OS=Sol...	[more]
W9SJ11_9ROSA	9.4e-38	97.6	Uncharacterized protein OS=Mor...	[more]

BLAST of Spo19206.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Spo19206.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 0


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR34410	FAMILY NOT NAMED	coord: 51..138 score: 4.6

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006506	GPI anchor biosynthetic process
biological_process	GO:0090305	nucleic acid phosphodiester bond hydrolysis
molecular_function	GO:0004519	endonuclease activity
molecular_function	GO:0016758	transferase activity, transferring hexosyl groups