Spo19397.1 (mRNA) | SpinachBase

Overview


Name	Spo19397.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	Cysteine protease ATG4B (3.4.22.-) (Autophagy-related protein 4 homolog B)
Location	SpoScf_62496 : 62 .. 574 (-)
Sequence length	513

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGAATTTGTTTGAGAGTACTGGTACTGCAAATTCATCTCCCGGTACTAGTTCCTCTTCAATTTCCCCTAATTTCAAACGCATTGCTTCTAATTCTTCCGATCACAAATCGTCATTATTGCCATCAACTAGTGCTTCGTCGTTGTTGTCTGGAATTGTTGTTTCCGCTTTCTCGATTTTTGAGCCTTGTGTGGAGGAGGGACTGGATACGGGTTCGAATTCTCGTGAGACGAAGGGGGTTGGAAATAAGACCAAGATTAGTAATTGTTGTAGTAGTAATGGTTGGGCGGCTGCTGTAAAGAGGGTTATGGCGAGTGGATCGATGAGGAGGATTAAGGAGCATATGTTCGGGTTTACTAAGTCTAGTGCTGTCACCTCTACAAGTGATATTTGGTTGTTGGGTGTTTGCTATAAACTTGATGATGCCATTGGTGGTGATCCTGTTAGTAGCAGCGGATTTGCTGCATTTGTCGAGGATTTTTCATCGAGGATTTTGATGACTTATAGGAAA

mRNA sequence

ATGAAGAATTTGTTTGAGAGTACTGGTACTGCAAATTCATCTCCCGGTACTAGTTCCTCTTCAATTTCCCCTAATTTCAAACGCATTGCTTCTAATTCTTCCGATCACAAATCGTCATTATTGCCATCAACTAGTGCTTCGTCGTTGTTGTCTGGAATTGTTGTTTCCGCTTTCTCGATTTTTGAGCCTTGTGTGGAGGAGGGACTGGATACGGGTTCGAATTCTCGTGAGACGAAGGGGGTTGGAAATAAGACCAAGATTAGTAATTGTTGTAGTAGTAATGGTTGGGCGGCTGCTGTAAAGAGGGTTATGGCGAGTGGATCGATGAGGAGGATTAAGGAGCATATGTTCGGGTTTACTAAGTCTAGTGCTGTCACCTCTACAAGTGATATTTGGTTGTTGGGTGTTTGCTATAAACTTGATGATGCCATTGGTGGTGATCCTGTTAGTAGCAGCGGATTTGCTGCATTTGTCGAGGATTTTTCATCGAGGATTTTGATGACTTATAGGAAA

Coding sequence (CDS)

ATGAAGAATTTGTTTGAGAGTACTGGTACTGCAAATTCATCTCCCGGTACTAGTTCCTCTTCAATTTCCCCTAATTTCAAACGCATTGCTTCTAATTCTTCCGATCACAAATCGTCATTATTGCCATCAACTAGTGCTTCGTCGTTGTTGTCTGGAATTGTTGTTTCCGCTTTCTCGATTTTTGAGCCTTGTGTGGAGGAGGGACTGGATACGGGTTCGAATTCTCGTGAGACGAAGGGGGTTGGAAATAAGACCAAGATTAGTAATTGTTGTAGTAGTAATGGTTGGGCGGCTGCTGTAAAGAGGGTTATGGCGAGTGGATCGATGAGGAGGATTAAGGAGCATATGTTCGGGTTTACTAAGTCTAGTGCTGTCACCTCTACAAGTGATATTTGGTTGTTGGGTGTTTGCTATAAACTTGATGATGCCATTGGTGGTGATCCTGTTAGTAGCAGCGGATTTGCTGCATTTGTCGAGGATTTTTCATCGAGGATTTTGATGACTTATAGGAAA

Protein sequence

MKNLFESTGTANSSPGTSSSSISPNFKRIASNSSDHKSSLLPSTSASSLLSGIVVSAFSIFEPCVEEGLDTGSNSRETKGVGNKTKISNCCSSNGWAAAVKRVMASGSMRRIKEHMFGFTKSSAVTSTSDIWLLGVCYKLDDAIGGDPVSSSGFAAFVEDFSSRILMTYRK

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo19397	Spo19397	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo19397.1	Spo19397.1-protein	polypeptide

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo19397.1.CDS.1	Spo19397.1.CDS.1	CDS

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo19397.1.exon.1	Spo19397.1.exon.1	exon

Homology

[Close]

BLAST of Spo19397.1 vs. NCBI nr
Match: gi|902151889|gb|KNA05568.1| (hypothetical protein SOVF_189160 isoform B [Spinacia oleracea])

HSP 1 Score: 326.6 bits (836), Expect = 2.500e-86
Identity = 171/171 (100.00%), Postives = 171/171 (100.00%), Query Frame = 1


		  
Query: 1   MKNLFESTGTANSSPGTSSSSISPNFKRIASNSSDHKSSLLPSTSASSLLSGIVVSAFSI 60
           MKNLFESTGTANSSPGTSSSSISPNFKRIASNSSDHKSSLLPSTSASSLLSGIVVSAFSI
Sbjct: 1   MKNLFESTGTANSSPGTSSSSISPNFKRIASNSSDHKSSLLPSTSASSLLSGIVVSAFSI 60

Query: 61  FEPCVEEGLDTGSNSRETKGVGNKTKISNCCSSNGWAAAVKRVMASGSMRRIKEHMFGFT 120
           FEPCVEEGLDTGSNSRETKGVGNKTKISNCCSSNGWAAAVKRVMASGSMRRIKEHMFGFT
Sbjct: 61  FEPCVEEGLDTGSNSRETKGVGNKTKISNCCSSNGWAAAVKRVMASGSMRRIKEHMFGFT 120

Query: 121 KSSAVTSTSDIWLLGVCYKLDDAIGGDPVSSSGFAAFVEDFSSRILMTYRK 172
           KSSAVTSTSDIWLLGVCYKLDDAIGGDPVSSSGFAAFVEDFSSRILMTYRK
Sbjct: 121 KSSAVTSTSDIWLLGVCYKLDDAIGGDPVSSSGFAAFVEDFSSRILMTYRK 171

[Close]

BLAST of Spo19397.1 vs. NCBI nr
Match: gi|902151888|gb|KNA05567.1| (hypothetical protein SOVF_189160 isoform A [Spinacia oleracea])

HSP 1 Score: 326.6 bits (836), Expect = 2.500e-86
Identity = 171/171 (100.00%), Postives = 171/171 (100.00%), Query Frame = 1


		  
Query: 1   MKNLFESTGTANSSPGTSSSSISPNFKRIASNSSDHKSSLLPSTSASSLLSGIVVSAFSI 60
           MKNLFESTGTANSSPGTSSSSISPNFKRIASNSSDHKSSLLPSTSASSLLSGIVVSAFSI
Sbjct: 1   MKNLFESTGTANSSPGTSSSSISPNFKRIASNSSDHKSSLLPSTSASSLLSGIVVSAFSI 60

Query: 61  FEPCVEEGLDTGSNSRETKGVGNKTKISNCCSSNGWAAAVKRVMASGSMRRIKEHMFGFT 120
           FEPCVEEGLDTGSNSRETKGVGNKTKISNCCSSNGWAAAVKRVMASGSMRRIKEHMFGFT
Sbjct: 61  FEPCVEEGLDTGSNSRETKGVGNKTKISNCCSSNGWAAAVKRVMASGSMRRIKEHMFGFT 120

Query: 121 KSSAVTSTSDIWLLGVCYKLDDAIGGDPVSSSGFAAFVEDFSSRILMTYRK 172
           KSSAVTSTSDIWLLGVCYKLDDAIGGDPVSSSGFAAFVEDFSSRILMTYRK
Sbjct: 121 KSSAVTSTSDIWLLGVCYKLDDAIGGDPVSSSGFAAFVEDFSSRILMTYRK 171

[Close]

BLAST of Spo19397.1 vs. NCBI nr
Match: gi|731323648|ref|XP_010672541.1| (PREDICTED: cysteine protease ATG4 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 237.3 bits (604), Expect = 2.000e-59
Identity = 135/174 (77.59%), Postives = 143/174 (82.18%), Query Frame = 1


		  
Query: 1   MKNLFESTGTANSSPGTSSSSISPNFKRIASNSSDHKSSLLPSTSASSLLSGIVVSAFSI 60
           MKNLFES GT NSS G +SS+ISP FK I SNSSDHKSS   ST ASSLLSGI VSAFSI
Sbjct: 1   MKNLFESAGTENSSSGATSSTISPKFKHICSNSSDHKSSS-SSTRASSLLSGIFVSAFSI 60

Query: 61  FEPCVEEGLDTGSNSR---ETKGVGNKTKISNCCSSNGWAAAVKRVMASGSMRRIKEHMF 120
           FE   EEG ++ SNSR   E KGVG K KISN  SSNGW  AVKR+MASGSMRRIKEHMF
Sbjct: 61  FEHRSEEGRNSSSNSRSSCEKKGVGAKNKISNS-SSNGWGTAVKRIMASGSMRRIKEHMF 120

Query: 121 GFTKSSAVTSTSDIWLLGVCYKLDDAIGGDPVSSSGFAAFVEDFSSRILMTYRK 172
           GF +SS + STSDIWLLGVCYKLDDA GGDPVSSSGFAAFVEDFSSRIL+TYRK
Sbjct: 121 GFNRSSIIASTSDIWLLGVCYKLDDATGGDPVSSSGFAAFVEDFSSRILLTYRK 172

[Close]

BLAST of Spo19397.1 vs. NCBI nr
Match: gi|728828696|gb|KHG08139.1| (Cysteine protease ATG4 [Gossypium arboreum])

HSP 1 Score: 108.2 bits (269), Expect = 1.400e-20
Identity = 63/127 (49.61%), Postives = 78/127 (61.42%), Query Frame = 1


		  
Query: 45  SASSLLSGIVVSAFSIFEPCVEEGLDTGSNSRETKGVGNKTKISNCCSSNGWAAAVKRVM 104
           S +SL S    SAFS+F        DT S S  +     K+  S    +NGW AAVKRV+
Sbjct: 74  SKTSLWSNFFASAFSVF--------DTYSESSSSSACEKKSSFSK---TNGWTAAVKRVV 133

Query: 105 ASGSMRRIKEHMFGFTKSSAVTSTSDIWLLGVCYKLDDAIGGDPVSSSGFAAFVEDFSSR 164
           + GSMRRI E + G +K    +STSDIWLLG+CYK+     GD  ++S  AAF +DFSSR
Sbjct: 134 SGGSMRRIHERVLGPSKIGISSSTSDIWLLGLCYKISQESSGDVDATSALAAFKQDFSSR 189

Query: 165 ILMTYRK 172
           ILMTYRK
Sbjct: 194 ILMTYRK 189

[Close]

BLAST of Spo19397.1 vs. NCBI nr
Match: gi|568849538|ref|XP_006478507.1| (PREDICTED: cysteine protease ATG4 isoform X1 [Citrus sinensis])

HSP 1 Score: 104.0 bits (258), Expect = 2.700e-19
Identity = 74/177 (41.81%), Postives = 103/177 (58.19%), Query Frame = 1


		  
Query: 1   MKNLFESTGTAN----SSPGTSSSSISPNFKRIASNSSDHKSSLLPSTSASSLLSGIVVS 60
           MK   E  G +     S+P T + S++     + S+ S         +S  SLLS +  S
Sbjct: 1   MKGFREKAGASKCFSKSTPDTPNRSLASVGSELGSSES--------KSSKGSLLSSLFNS 60

Query: 61  AFSIFEPCVEEGLDTGSNSRETKGVGNKTKISNCCSSNGWAAAVKRVMASGSMRRIKEHM 120
           AFS+FE   E      S++ E K V NK        SNGW AAVKR++ +GSMRRI E +
Sbjct: 61  AFSVFETYSE------SSANEKKAVHNK--------SNGWTAAVKRLVTAGSMRRIHERV 120

Query: 121 FGFTKSSAVTSTSDIWLLGVCYKL--DDAIGGDPVSSSGFAAFVEDFSSRILMTYRK 172
            G +++   +STSDIWLLGVC+K+  D+A+ GD   ++G A F +DFSSRIL++YRK
Sbjct: 121 LGPSRTGISSSTSDIWLLGVCHKIAQDEAL-GDAAGNNGLAEFNQDFSSRILISYRK 154

[Close]

BLAST of Spo19397.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QE75_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_189160 PE=3 SV=1)

HSP 1 Score: 326.6 bits (836), Expect = 1.800e-86
Identity = 171/171 (100.00%), Postives = 171/171 (100.00%), Query Frame = 1


		  
Query: 1   MKNLFESTGTANSSPGTSSSSISPNFKRIASNSSDHKSSLLPSTSASSLLSGIVVSAFSI 60
           MKNLFESTGTANSSPGTSSSSISPNFKRIASNSSDHKSSLLPSTSASSLLSGIVVSAFSI
Sbjct: 1   MKNLFESTGTANSSPGTSSSSISPNFKRIASNSSDHKSSLLPSTSASSLLSGIVVSAFSI 60

Query: 61  FEPCVEEGLDTGSNSRETKGVGNKTKISNCCSSNGWAAAVKRVMASGSMRRIKEHMFGFT 120
           FEPCVEEGLDTGSNSRETKGVGNKTKISNCCSSNGWAAAVKRVMASGSMRRIKEHMFGFT
Sbjct: 61  FEPCVEEGLDTGSNSRETKGVGNKTKISNCCSSNGWAAAVKRVMASGSMRRIKEHMFGFT 120

Query: 121 KSSAVTSTSDIWLLGVCYKLDDAIGGDPVSSSGFAAFVEDFSSRILMTYRK 172
           KSSAVTSTSDIWLLGVCYKLDDAIGGDPVSSSGFAAFVEDFSSRILMTYRK
Sbjct: 121 KSSAVTSTSDIWLLGVCYKLDDAIGGDPVSSSGFAAFVEDFSSRILMTYRK 171

[Close]

BLAST of Spo19397.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QFV1_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_189160 PE=3 SV=1)

HSP 1 Score: 326.6 bits (836), Expect = 1.800e-86
Identity = 171/171 (100.00%), Postives = 171/171 (100.00%), Query Frame = 1


		  
Query: 1   MKNLFESTGTANSSPGTSSSSISPNFKRIASNSSDHKSSLLPSTSASSLLSGIVVSAFSI 60
           MKNLFESTGTANSSPGTSSSSISPNFKRIASNSSDHKSSLLPSTSASSLLSGIVVSAFSI
Sbjct: 1   MKNLFESTGTANSSPGTSSSSISPNFKRIASNSSDHKSSLLPSTSASSLLSGIVVSAFSI 60

Query: 61  FEPCVEEGLDTGSNSRETKGVGNKTKISNCCSSNGWAAAVKRVMASGSMRRIKEHMFGFT 120
           FEPCVEEGLDTGSNSRETKGVGNKTKISNCCSSNGWAAAVKRVMASGSMRRIKEHMFGFT
Sbjct: 61  FEPCVEEGLDTGSNSRETKGVGNKTKISNCCSSNGWAAAVKRVMASGSMRRIKEHMFGFT 120

Query: 121 KSSAVTSTSDIWLLGVCYKLDDAIGGDPVSSSGFAAFVEDFSSRILMTYRK 172
           KSSAVTSTSDIWLLGVCYKLDDAIGGDPVSSSGFAAFVEDFSSRILMTYRK
Sbjct: 121 KSSAVTSTSDIWLLGVCYKLDDAIGGDPVSSSGFAAFVEDFSSRILMTYRK 171

[Close]

BLAST of Spo19397.1 vs. UniProtKB/TrEMBL
Match: A0A0J8CPD3_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_3g059550 PE=3 SV=1)

HSP 1 Score: 237.3 bits (604), Expect = 1.400e-59
Identity = 135/174 (77.59%), Postives = 143/174 (82.18%), Query Frame = 1


		  
Query: 1   MKNLFESTGTANSSPGTSSSSISPNFKRIASNSSDHKSSLLPSTSASSLLSGIVVSAFSI 60
           MKNLFES GT NSS G +SS+ISP FK I SNSSDHKSS   ST ASSLLSGI VSAFSI
Sbjct: 1   MKNLFESAGTENSSSGATSSTISPKFKHICSNSSDHKSSS-SSTRASSLLSGIFVSAFSI 60

Query: 61  FEPCVEEGLDTGSNSR---ETKGVGNKTKISNCCSSNGWAAAVKRVMASGSMRRIKEHMF 120
           FE   EEG ++ SNSR   E KGVG K KISN  SSNGW  AVKR+MASGSMRRIKEHMF
Sbjct: 61  FEHRSEEGRNSSSNSRSSCEKKGVGAKNKISNS-SSNGWGTAVKRIMASGSMRRIKEHMF 120

Query: 121 GFTKSSAVTSTSDIWLLGVCYKLDDAIGGDPVSSSGFAAFVEDFSSRILMTYRK 172
           GF +SS + STSDIWLLGVCYKLDDA GGDPVSSSGFAAFVEDFSSRIL+TYRK
Sbjct: 121 GFNRSSIIASTSDIWLLGVCYKLDDATGGDPVSSSGFAAFVEDFSSRILLTYRK 172

[Close]

BLAST of Spo19397.1 vs. UniProtKB/TrEMBL
Match: A0A0B0N8F2_GOSAR (Cysteine protease ATG4 OS=Gossypium arboreum GN=F383_05577 PE=3 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 9.900e-21
Identity = 63/127 (49.61%), Postives = 78/127 (61.42%), Query Frame = 1


		  
Query: 45  SASSLLSGIVVSAFSIFEPCVEEGLDTGSNSRETKGVGNKTKISNCCSSNGWAAAVKRVM 104
           S +SL S    SAFS+F        DT S S  +     K+  S    +NGW AAVKRV+
Sbjct: 74  SKTSLWSNFFASAFSVF--------DTYSESSSSSACEKKSSFSK---TNGWTAAVKRVV 133

Query: 105 ASGSMRRIKEHMFGFTKSSAVTSTSDIWLLGVCYKLDDAIGGDPVSSSGFAAFVEDFSSR 164
           + GSMRRI E + G +K    +STSDIWLLG+CYK+     GD  ++S  AAF +DFSSR
Sbjct: 134 SGGSMRRIHERVLGPSKIGISSSTSDIWLLGLCYKISQESSGDVDATSALAAFKQDFSSR 189

Query: 165 ILMTYRK 172
           ILMTYRK
Sbjct: 194 ILMTYRK 189

[Close]

BLAST of Spo19397.1 vs. UniProtKB/TrEMBL
Match: A0A067DBF9_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g011418mg PE=3 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 2.400e-19
Identity = 74/177 (41.81%), Postives = 103/177 (58.19%), Query Frame = 1


		  
Query: 1   MKNLFESTGTAN----SSPGTSSSSISPNFKRIASNSSDHKSSLLPSTSASSLLSGIVVS 60
           MK   E  G +     S+P T + S++     + S+ S         +S  SLLS +  S
Sbjct: 1   MKGFREKAGASKCFSKSTPDTPNRSLASVGSELGSSES--------KSSKGSLLSSLFNS 60

Query: 61  AFSIFEPCVEEGLDTGSNSRETKGVGNKTKISNCCSSNGWAAAVKRVMASGSMRRIKEHM 120
           AFS+FE   E      S++ E K V NK        SNGW AAVKR++ +GSMRRI E +
Sbjct: 61  AFSVFETYSE------SSASEKKAVHNK--------SNGWTAAVKRLVTAGSMRRIHERV 120

Query: 121 FGFTKSSAVTSTSDIWLLGVCYKL--DDAIGGDPVSSSGFAAFVEDFSSRILMTYRK 172
            G +++   +STSDIWLLGVC+K+  D+A+ GD   ++G A F +DFSSRIL++YRK
Sbjct: 121 LGPSRTGISSSTSDIWLLGVCHKIAQDEAL-GDAAGNNGLAEFNQDFSSRILISYRK 154

[Close]

BLAST of Spo19397.1 vs. ExPASy Swiss-Prot
Match: ATG4A_ARATH (Cysteine protease ATG4a OS=Arabidopsis thaliana GN=ATG4A PE=2 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 4.000e-15
Identity = 60/151 (39.74%), Postives = 82/151 (54.30%), Query Frame = 1


		  
Query: 30  ASNSSDHKSSLLPSTSASS-------LLSGIVVSAFSIFEPCVEEGLDTGSNSRETKGVG 89
           +S S  H  S L S S  S       L S +  S+ S+ +P            RE+   G
Sbjct: 16  SSKSDTHDKSPLVSDSGPSDNKSKFTLWSNVFTSSSSVSQPY-----------RESSTSG 75

Query: 90  NKTKISNCCSSNGWAAAVKRV-MASGSMRRIKEHMFGFTKSSAVTSTSDIWLLGVCYKLD 149
           +K     C + NGW A VKRV MASG++RR +E + G  ++   ++TSD+WLLGVCYK+ 
Sbjct: 76  HKQV---CTTRNGWTAFVKRVSMASGAIRRFQERVLGPNRTGLPSTTSDVWLLGVCYKIS 135

Query: 150 -DAIGGDPVSSSGFAAFVEDFSSRILMTYRK 172
            D   G+  + +  AA   DFSS+ILMTYRK
Sbjct: 136 ADENSGETDTGTVLAALQLDFSSKILMTYRK 152

[Close]

BLAST of Spo19397.1 vs. ExPASy Swiss-Prot
Match: ATG4_MEDTR (Cysteine protease ATG4 OS=Medicago truncatula GN=ATG4 PE=3 SV=1)

HSP 1 Score: 80.1 bits (196), Expect = 2.600e-14
Identity = 65/172 (37.79%), Postives = 93/172 (54.07%), Query Frame = 1


		  
Query: 1   MKNLFESTGTANSSPGTSSSSISPNFKRIASNSSDHKSSLLPSTSASSLLSGIVVSAFSI 60
           +K+L +    A  S   SS+ I  N +  AS+ +    S  P    +SL S    S FS+
Sbjct: 3   LKDLCDRIVAAKCS-SKSSTEIVDNTQVPASSKAGSSDSKFPK---ASLWSTFFTSGFSV 62

Query: 61  FEPCVEEGLDTGSNSRETKGVGNKTKISNCCSSNGWAAAVKRVMASGSMRRIKEHMFGFT 120
            E   E      S+S E K V ++        ++GWAAAV++V++ GSMRR +E + G  
Sbjct: 63  DETYSE------SSSSEKKTVHSR--------NSGWAAAVRKVVSGGSMRRFQERVLGSC 122

Query: 121 KSSAVTSTSDIWLLGVCYKLDD-AIGGDPVSSSGFAAFVEDFSSRILMTYRK 172
           ++   +S  DIWLLGVC+K+      GD    + FAAF +DF SRIL+TYRK
Sbjct: 123 RTDVSSSDGDIWLLGVCHKISQHESTGDVDIRNVFAAFEQDFFSRILITYRK 156

[Close]

BLAST of Spo19397.1 vs. ExPASy Swiss-Prot
Match: ATG4B_ORYSJ (Cysteine protease ATG4B OS=Oryza sativa subsp. japonica GN=ATG4B PE=2 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 2.100e-11
Identity = 60/160 (37.50%), Postives = 82/160 (51.25%), Query Frame = 1


		  
Query: 16  GTSSSSISPNFK-RIA--SNSSDHKSSLLPSTSASSLLSGIVVSAFSIFEPCVEEGLDTG 75
           G SSSS  P  +  IA  S+SS+ K       S +S+LS +  S F+IFE       D+ 
Sbjct: 8   GVSSSSSDPLCEGNIAPCSSSSEQKEDCSLKQSKTSILSCVFNSPFNIFEA----HQDSS 67

Query: 76  SNSRETKGVGNKTKISNCCSSNGWAAAVKRVMASGSMRRIKEHMFGFTKSSAVTSTSDIW 135
           +N       G          S  W+  ++R++ SGSM R       F  +S V ++SD+W
Sbjct: 68  ANKSPKSSSG----------SYDWSRVLRRIVCSGSMWR-------FLGTSKVLTSSDVW 127

Query: 136 LLGVCYKLD-DAIGGDPVSSSGFAAFVEDFSSRILMTYRK 172
            LG CYKL  +    D  S SG A F+EDFSSRI +TYR+
Sbjct: 128 FLGKCYKLSSEESSSDSDSESGHATFLEDFSSRIWITYRR 146

[Close]

BLAST of Spo19397.1 vs. ExPASy Swiss-Prot
Match: ATG4B_ORYSI (Cysteine protease ATG4B OS=Oryza sativa subsp. indica GN=ATG4B PE=1 SV=2)

HSP 1 Score: 69.7 bits (169), Expect = 3.500e-11
Identity = 60/160 (37.50%), Postives = 81/160 (50.62%), Query Frame = 1


		  
Query: 16  GTSSSSISPNFK-RIA--SNSSDHKSSLLPSTSASSLLSGIVVSAFSIFEPCVEEGLDTG 75
           G SSSS  P  +  IA  S+SS+ K       S +S+LS +  S F+IFE       D+ 
Sbjct: 8   GVSSSSSDPLCEGNIAPCSSSSEQKEDCSLKQSKTSILSCVFNSPFNIFEA----HQDSS 67

Query: 76  SNSRETKGVGNKTKISNCCSSNGWAAAVKRVMASGSMRRIKEHMFGFTKSSAVTSTSDIW 135
           +N       G          S  W   ++R++ SGSM R       F  +S V ++SD+W
Sbjct: 68  ANKSPKSSSG----------SYDWLRVLRRIVCSGSMWR-------FLGTSKVLTSSDVW 127

Query: 136 LLGVCYKLD-DAIGGDPVSSSGFAAFVEDFSSRILMTYRK 172
            LG CYKL  +    D  S SG A F+EDFSSRI +TYR+
Sbjct: 128 FLGKCYKLSSEESSSDSDSESGHATFLEDFSSRIWITYRR 146

[Close]

BLAST of Spo19397.1 vs. ExPASy Swiss-Prot
Match: ATG4B_ARATH (Cysteine protease ATG4b OS=Arabidopsis thaliana GN=ATG4B PE=1 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 1.300e-10
Identity = 55/169 (32.54%), Postives = 85/169 (50.30%), Query Frame = 1


		  
Query: 5   FESTGTANSSPGTSSSSISPNFKRIASNSSDHKSSLLPSTSASSLLSGIVVSAFSIFEPC 64
           F  +  ++SS        SP      S SSD+KS+L       +L S +V S+  + + C
Sbjct: 8   FVPSKCSSSSTSEKRDISSPTSLVSDSASSDNKSNL-------TLCSDVVASSSPVSQLC 67

Query: 65  VEEGLDTGSNSRETKGVGNKTKISNCCSSNGWAAAVKRV-MASGSMRRIKEHMFGFTKSS 124
             E   +G N               C + + W   +K   MASG++RR ++ + G +++ 
Sbjct: 68  -REASTSGHNPV-------------CTTHSSWTVILKTASMASGAIRRFQDRVLGPSRTG 127

Query: 125 AVTSTSDIWLLGVCYKLDDAIGGDPVSSSG-FAAFVEDFSSRILMTYRK 172
             +STS+IWLLGVCYK+ +    +   +    AAF +DFSS ILMTYR+
Sbjct: 128 ISSSTSEIWLLGVCYKISEGESSEEADAGRVLAAFRQDFSSLILMTYRR 155

[Close]

BLAST of Spo19397.1 vs. TAIR (Arabidopsis)
Match: AT2G44140.1 (Peptidase family C54 protein)

HSP 1 Score: 82.8 bits (203), Expect = 2.200e-16
Identity = 60/151 (39.74%), Postives = 82/151 (54.30%), Query Frame = 1


		  
Query: 30  ASNSSDHKSSLLPSTSASS-------LLSGIVVSAFSIFEPCVEEGLDTGSNSRETKGVG 89
           +S S  H  S L S S  S       L S +  S+ S+ +P            RE+   G
Sbjct: 16  SSKSDTHDKSPLVSDSGPSDNKSKFTLWSNVFTSSSSVSQPY-----------RESSTSG 75

Query: 90  NKTKISNCCSSNGWAAAVKRV-MASGSMRRIKEHMFGFTKSSAVTSTSDIWLLGVCYKLD 149
           +K     C + NGW A VKRV MASG++RR +E + G  ++   ++TSD+WLLGVCYK+ 
Sbjct: 76  HKQV---CTTRNGWTAFVKRVSMASGAIRRFQERVLGPNRTGLPSTTSDVWLLGVCYKIS 135

Query: 150 -DAIGGDPVSSSGFAAFVEDFSSRILMTYRK 172
            D   G+  + +  AA   DFSS+ILMTYRK
Sbjct: 136 ADENSGETDTGTVLAALQLDFSSKILMTYRK 152

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BLAST of Spo19397.1 vs. TAIR (Arabidopsis)
Match: AT3G59950.1 (Peptidase family C54 protein)

HSP 1 Score: 67.8 bits (164), Expect = 7.500e-12
Identity = 55/169 (32.54%), Postives = 85/169 (50.30%), Query Frame = 1


		  
Query: 5   FESTGTANSSPGTSSSSISPNFKRIASNSSDHKSSLLPSTSASSLLSGIVVSAFSIFEPC 64
           F  +  ++SS        SP      S SSD+KS+L       +L S +V S+  + + C
Sbjct: 8   FVPSKCSSSSTSEKRDISSPTSLVSDSASSDNKSNL-------TLCSDVVASSSPVSQLC 67

Query: 65  VEEGLDTGSNSRETKGVGNKTKISNCCSSNGWAAAVKRV-MASGSMRRIKEHMFGFTKSS 124
             E   +G N               C + + W   +K   MASG++RR ++ + G +++ 
Sbjct: 68  -REASTSGHNPV-------------CTTHSSWTVILKTASMASGAIRRFQDRVLGPSRTG 127

Query: 125 AVTSTSDIWLLGVCYKLDDAIGGDPVSSSG-FAAFVEDFSSRILMTYRK 172
             +STS+IWLLGVCYK+ +    +   +    AAF +DFSS ILMTYR+
Sbjct: 128 ISSSTSEIWLLGVCYKISEGESSEEADAGRVLAAFRQDFSSLILMTYRR 155

The following BLAST results are available for this feature:

BLAST of Spo19397.1 vs. NCBI nr
BLAST of Spo19397.1 vs. UniProtKB/TrEMBL
BLAST of Spo19397.1 vs. ExPASy Swiss-Prot
BLAST of Spo19397.1 vs. TAIR (Arabidopsis)

BLAST of Spo19397.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902151889\|gb\|KNA05568.1\|	2.5e-86	100.	hypothetical protein SOVF_1891...	[more]
gi\|902151888\|gb\|KNA05567.1\|	2.5e-86	100.	hypothetical protein SOVF_1891...	[more]
gi\|731323648\|ref\|XP_010672541.1\|	2.0e-59	77.5	PREDICTED: cysteine protease A...	[more]
gi\|728828696\|gb\|KHG08139.1\|	1.4e-20	49.6	Cysteine protease ATG4 [Gossyp...	[more]
gi\|568849538\|ref\|XP_006478507.1\|	2.7e-19	41.8	PREDICTED: cysteine protease A...	[more]

BLAST of Spo19397.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9QE75_SPIOL	1.8e-86	100.	Uncharacterized protein OS=Spi...	[more]
A0A0K9QFV1_SPIOL	1.8e-86	100.	Uncharacterized protein OS=Spi...	[more]
A0A0J8CPD3_BETVU	1.4e-59	77.5	Uncharacterized protein OS=Bet...	[more]
A0A0B0N8F2_GOSAR	9.9e-21	49.6	Cysteine protease ATG4 OS=Goss...	[more]
A0A067DBF9_CITSI	2.4e-19	41.8	Uncharacterized protein OS=Cit...	[more]

BLAST of Spo19397.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
ATG4A_ARATH	4.0e-15	39.7	Cysteine protease ATG4a OS=Ara...	[more]
ATG4_MEDTR	2.6e-14	37.7	Cysteine protease ATG4 OS=Medi...	[more]
ATG4B_ORYSJ	2.1e-11	37.5	Cysteine protease ATG4B OS=Ory...	[more]
ATG4B_ORYSI	3.5e-11	37.5	Cysteine protease ATG4B OS=Ory...	[more]
ATG4B_ARATH	1.3e-10	32.5	Cysteine protease ATG4b OS=Ara...	[more]

BLAST of Spo19397.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 2


Match Name	E-value	Identity	Description
AT2G44140.1	2.2e-16	39.7	Peptidase family C54 protein	[more]
AT3G59950.1	7.5e-12	32.5	Peptidase family C54 protein	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005078	Peptidase C54	PANTHER	PTHR22624	APG4 AUTOPHAGY 4-RELATED	coord: 13..171 score: 2.3
None	No IPR available	PANTHER	PTHR22624:SF34	AUTOPHAGY-SPECIFIC GENE 4, ISOFORM A	coord: 13..171 score: 2.3

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016787	hydrolase activity