Spo19414.1 (mRNA) | SpinachBase

Overview


Name	Spo19414.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	(Spermidine synthase, putative) (2.5.1.16)
Location	chr1 : 33821897 .. 33826056 (+)
Sequence length	1193

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CTCTCTAGTGATCATCATATACATTTTCAATTCTCTCTCTAAGTTACTTATACCAAAAAATGGGTACTGAAGCTATTGAGATCATAACTTCAAATGCTTTGTCAGCCATTTTTGATAAACAAAACCGTTTCAATCAAGATAACAATGAGTTTTGGTATGAGGAAGAAATTGATGAGGATCTCAAATGGTCCTTCGCTTTGAAGAGGTTTGATTTTATTTTACTATCATCTTTTATTTTCAATTGACTATTTAATTAATCAAAATTCCGGAATTTGAGTGTTGGATCGGAGAAGTTTCAGGTCGCATTTCCATTGAAATTCAATGAATGGTTGTGAATTTGTCATATATTCTTGAAATTCGTATGTCAACTTCATTCCATTAAATTAAATGATTTGTTAATCTTGTCATCCAGCGTGCTGCAAAAAGCCACTAGCGAATACCAGGACATTGTGCTACTCGACACTAAGAAATTTGGGAAGGTTTGTACATCAAAAATAAATAAATAAATAAAAAAAATTATCTTTTGTATTTGATTTTTTATTTAATTTGAAAAATTCTTGTATAGTTCTCGATCAATGCTCCTATCGTCATCACGATGAGATAGACTTATAGTTTCCGTAATCTCTAAAAAAGTAAAAATGGTATATATTAGCGTGGCGGGACTATATTTGAGAATTACATTGGTCGTGGACCATGCATAAATTCTTCTTCTTTTATATAGATTCCTTTTTCTTTTTTCGTCGAAAAAAGTTGGTATATCTTTTACGGCACTAGTTTGCTATTTTGGATGATGATTTTTTGTATGTATATATATGTATAGGTGTTGGTGCTTGATGGTAAGATGCAAAGTGCGGAGGCTGATGAATTTATCTACCATGAGTGCTTGATTCACCCTGCCCTCCTTTGTCATCTAAAGTAAGTACAACTCCTATAACTCAATTATAACTTTATTAATTAGGGTGTTTTCTATTCATCTGATTTTCACTAATTTTTTCTGAACTTATCTTATCTGAACTTATCTTATGTGAACTTATCTGAACTCATTTTTCCCATAATAAAAGAAAATAAGGTGAATAGAACAGAGCCTTAGATGAATCATTTCAAGTTTACAAGGAAATAAAATAAACGATTAATTGTTAACAAAATCTAAAACTCGATCAAATTTGTTGCATGCACAATCAATCATCAATGTATTGAATATTTATATGCATCAAAATTGTATTTGATTATTTACATGCAATAGTTTCTACCGCCCTCCAATACTAAAATGAAGTAAGAGCACAATAGAAAAATCAAATGTTAAATGATTACTATACCAAGAAAAGTGATAACTTTTTCCATATTAGCGAACATATATATTATACAATATGTGAGAAGTAATTTCAATTTGGACGAAAGTAGAGTATATAAGTTGCAACTTAATTAGGAGGACGGCGGAAAAAATAATTCTTTCATTAAGGCATATATTCTCATAAAAATCCAAAAAACTTAATAGTAGGTCGCATACCACTATCCAGTATCCACTATAGTTGATGATTATATGAAAACTTTAGAAACAGTTAATAATGTGGCATTTTGTCGCCTGTCTCATTCGCATTTCTGGTTAAGCTCATAATCTATAACATGCATGCGCGCAATTATTATTTCCCTGATATGCATATAGAGTTATAGACATATAGTATATGATTATATAGAATGAAATATATCGTGGTACTCCATAGTACACTAGTACTTATTGTAGTACATATGTAGCGATTTCACACATGTCGTGCTCTAATTGGCTCGAAATTATACAGAATAAGTGACTACTCCGTATATATGATTAAGTTTAACTAATGAGTATTGAGTATTGAGTATTGAGTATTGACATTTGTTAACTAATGATGACAGCCCAAAATCTATGTTCATAATGGGAGGTGGTGAAGGTTCTGCTGCTAGAGAAGCTCTTAAGCACAAGTCCATTGACAAAGTGGTCATGTGTGATATTGATCAGGTAAGAGTATAAGACATACATACCCGTCATCATGATCAGTGTCGGATTCCAAACTTTTATTAAATTACTCCGTATTACTCCCTCCGTCCCGGAATACTTGACCTGTTTTCCTTATCGGGCCGTCCCTTAATACTTGACCTGTTTCTAAAAATGGAAATATTCTAACAATATTATATTATTTCTCACTCCACCCCTATTAACCCACCTACCCCCTACTCCATACAAAAAATAATTAAAAATTCAACCCCTACTCTCCCCCAACCCCACCTCTTAACCCACATCCCACTAACTACATTAAAATAATACCCCACTATCAACTACTACCTATTAAATTAAATAAGTCAATTCAAGTCCCTTAAACTCTGTGCCGGTCAAACCGGGTCGAGTATTCCGGGACGGAGGGAGTAGAAAAGTAAAACGGATTATACTGTATTTATTTATTTAAGGAAGGTGAATAAGTAATACTACGTATGTAGTAACACATAACATACTTGTACTACATACTATATTATGGTAATGTATAATGTGCTTAATTAATTTCGTTACTCATGACAGGAGGTGGTTGATTTCTGCCGTAAACACTTGACAATCAACCAAGAAGCCTTCAACAATAACAAGTTTGAGCTTGTGATCAATGATGCAAAGTAAGTACATTTGATGTATTGGGATTTGGGAACTTTCAAATTTCAATAATAAAATAGTACAACTTTTAAAAATTTGAAACAAGTGTAATTTCTTACATGTAACGAAATGGGCGCGTATTGAACCCTACTCGAAACTGAAAGTAGAATATTGTTTGATTGTACAGGGCTGAATTGGAAGGAAGGAAGGAGAAATTTGATATAATAGTTGGAGATTTGGCAGACCCAGTTGAAGGAGGACCTTGTTACCAACTTTACACCAAATCTTTCTATGAGACTATTCTCAAACCTAGGCTTACTGATAATGGCATCTTTGTCACTCAGGTTTGATTACTGTCATTTTTTGATTTACAGTAAAAATACTTTGTAATTCCAAAATTTACTGTATTTTTTTATTTAAATACTTTGTAATTCCAAAATTTCACACTAATTAATGATTCTTTTTATAGGCTGGGCCTGCAGGAATTTACACCCACAAGGAGGTGTTCTCTTCCATTTACAACACCATCAGACAAGTCTTCAAATGTAAGCCTTTAGTGAGTTTAGTCTCCCAAATAATGTTACAAGGGAGACCGGTTTCTACAAATGGGTCGGACTATAACTTGACATTGGTGGCATGGCCCAACGGCCAAGATGCACGGATATAAGACATAGACATAGTACACTTTAAGTGTCCGGATAAAATTGTCACATTTCACATAGTACACTTTAAGGGCCCGGATAAAATTGTCACATTTCACATAGTACACTTTAAGGGCCCGGATAAAATTGTCGCATTTCCTAAGTCCTCCGCAATGGTTTGTAATTGAAATTATCTACTAGTTTTCAAGTTTGTTTGTTTTGCTTTCTGCAGATGTAGTTGCATTTACTGCTCATGTGCCATCGTATGCAGATACTTGGGGATGGGTTATGGTATGGACATGAAATCTCTTTAAGTTTTGTTAATGTAAATGATAATTTAGTTAGATTAATTTATAAGTGCATATTAATTTGTAATTAGGCCTCGGATCATCCAATTCTCATTGATGCTAAAGATCTCGATAAGAAGATCCAAGATCGGATTGATGGGGAGTTGCTATACCTGAATGGGACTTCCATACTTTCATCCACTTCCCTAAACAAAACTCTCTCTCAATCGTAAGCTGACTAACATTCTTGTATTTTGTTTACAAATTTCGAGTATATAATTTCCGATTTCCTTTTGTTAGGATTAATTGTGTTTTACCTCCTATAACTCTGTCAGGCTGTTGCAAGAAACTCATATCTATACTGAAGAAAGTGCAAGATTTCTGCATGGTCATGGGCTAGTTAATCGTCTCTAAGGATTGCAGAAGGGCGCGCATCAACTGGATTAAGGGCAATTAATGTCAAATGTTGATCAATAGAATAGTAACTTCAGTGGGCTATTCTTCTCTATAAGTAGGGTTCTTGTAAGGGGTTGTTGTTGTTGTAGAAATTATATTAGTAGCGCACATGGAAGAAAAGAAAGAAAGAAAGAAAGAACTGTCTTTAACTTTATTTTATGA

mRNA sequence

CTCTCTAGTGATCATCATATACATTTTCAATTCTCTCTCTAAGTTACTTATACCAAAAAATGGGTACTGAAGCTATTGAGATCATAACTTCAAATGCTTTGTCAGCCATTTTTGATAAACAAAACCGTTTCAATCAAGATAACAATGAGTTTTGGTATGAGGAAGAAATTGATGAGGATCTCAAATGGTCCTTCGCTTTGAAGAGCGTGCTGCAAAAAGCCACTAGCGAATACCAGGACATTGTGCTACTCGACACTAAGAAATTTGGGAAGGTGTTGGTGCTTGATGGTAAGATGCAAAGTGCGGAGGCTGATGAATTTATCTACCATGAGTGCTTGATTCACCCTGCCCTCCTTTGTCATCTAAACCCAAAATCTATGTTCATAATGGGAGGTGGTGAAGGTTCTGCTGCTAGAGAAGCTCTTAAGCACAAGTCCATTGACAAAGTGGTCATGTGTGATATTGATCAGGAGGTGGTTGATTTCTGCCGTAAACACTTGACAATCAACCAAGAAGCCTTCAACAATAACAAGTTTGAGCTTGTGATCAATGATGCAAAGGCTGAATTGGAAGGAAGGAAGGAGAAATTTGATATAATAGTTGGAGATTTGGCAGACCCAGTTGAAGGAGGACCTTGTTACCAACTTTACACCAAATCTTTCTATGAGACTATTCTCAAACCTAGGCTTACTGATAATGGCATCTTTGTCACTCAGGCTGGGCCTGCAGGAATTTACACCCACAAGGAGGTGTTCTCTTCCATTTACAACACCATCAGACAAGTCTTCAAATATGTAGTTGCATTTACTGCTCATGTGCCATCGTATGCAGATACTTGGGGATGGGTTATGGCCTCGGATCATCCAATTCTCATTGATGCTAAAGATCTCGATAAGAAGATCCAAGATCGGATTGATGGGGAGTTGCTATACCTGAATGGGACTTCCATACTTTCATCCACTTCCCTAAACAAAACTCTCTCTCAATCGCTGTTGCAAGAAACTCATATCTATACTGAAGAAAGTGCAAGATTTCTGCATGGTCATGGGCTAAATAGTAACTTCAGTGGGCTATTCTTCTCTATAAGTAGGGTTCTTGTAAGGGGTTGTTGTTGTTGTAGAAATTATATTAGTAGCGCACATGGAAGAAAAGAAAGAAAGAAAGAAAGAACTGTCTTTAACTTTATTTTATGA

Coding sequence (CDS)

ATGGGTACTGAAGCTATTGAGATCATAACTTCAAATGCTTTGTCAGCCATTTTTGATAAACAAAACCGTTTCAATCAAGATAACAATGAGTTTTGGTATGAGGAAGAAATTGATGAGGATCTCAAATGGTCCTTCGCTTTGAAGAGCGTGCTGCAAAAAGCCACTAGCGAATACCAGGACATTGTGCTACTCGACACTAAGAAATTTGGGAAGGTGTTGGTGCTTGATGGTAAGATGCAAAGTGCGGAGGCTGATGAATTTATCTACCATGAGTGCTTGATTCACCCTGCCCTCCTTTGTCATCTAAACCCAAAATCTATGTTCATAATGGGAGGTGGTGAAGGTTCTGCTGCTAGAGAAGCTCTTAAGCACAAGTCCATTGACAAAGTGGTCATGTGTGATATTGATCAGGAGGTGGTTGATTTCTGCCGTAAACACTTGACAATCAACCAAGAAGCCTTCAACAATAACAAGTTTGAGCTTGTGATCAATGATGCAAAGGCTGAATTGGAAGGAAGGAAGGAGAAATTTGATATAATAGTTGGAGATTTGGCAGACCCAGTTGAAGGAGGACCTTGTTACCAACTTTACACCAAATCTTTCTATGAGACTATTCTCAAACCTAGGCTTACTGATAATGGCATCTTTGTCACTCAGGCTGGGCCTGCAGGAATTTACACCCACAAGGAGGTGTTCTCTTCCATTTACAACACCATCAGACAAGTCTTCAAATATGTAGTTGCATTTACTGCTCATGTGCCATCGTATGCAGATACTTGGGGATGGGTTATGGCCTCGGATCATCCAATTCTCATTGATGCTAAAGATCTCGATAAGAAGATCCAAGATCGGATTGATGGGGAGTTGCTATACCTGAATGGGACTTCCATACTTTCATCCACTTCCCTAAACAAAACTCTCTCTCAATCGCTGTTGCAAGAAACTCATATCTATACTGAAGAAAGTGCAAGATTTCTGCATGGTCATGGGCTAAATAGTAACTTCAGTGGGCTATTCTTCTCTATAAGTAGGGTTCTTGTAAGGGGTTGTTGTTGTTGTAGAAATTATATTAGTAGCGCACATGGAAGAAAAGAAAGAAAGAAAGAAAGAACTGTCTTTAACTTTATTTTATGA

Protein sequence

MGTEAIEIITSNALSAIFDKQNRFNQDNNEFWYEEEIDEDLKWSFALKSVLQKATSEYQDIVLLDTKKFGKVLVLDGKMQSAEADEFIYHECLIHPALLCHLNPKSMFIMGGGEGSAAREALKHKSIDKVVMCDIDQEVVDFCRKHLTINQEAFNNNKFELVINDAKAELEGRKEKFDIIVGDLADPVEGGPCYQLYTKSFYETILKPRLTDNGIFVTQAGPAGIYTHKEVFSSIYNTIRQVFKYVVAFTAHVPSYADTWGWVMASDHPILIDAKDLDKKIQDRIDGELLYLNGTSILSSTSLNKTLSQSLLQETHIYTEESARFLHGHGLNSNFSGLFFSISRVLVRGCCCCRNYISSAHGRKERKKERTVFNFIL

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo19414	Spo19414	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo19414.1	Spo19414.1-protein	polypeptide

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo19414.1.exon.1	Spo19414.1.exon.1	exon
Spo19414.1.exon.2	Spo19414.1.exon.2	exon
Spo19414.1.exon.3	Spo19414.1.exon.3	exon
Spo19414.1.exon.4	Spo19414.1.exon.4	exon
Spo19414.1.exon.5	Spo19414.1.exon.5	exon
Spo19414.1.exon.6	Spo19414.1.exon.6	exon
Spo19414.1.exon.7	Spo19414.1.exon.7	exon
Spo19414.1.exon.8	Spo19414.1.exon.8	exon
Spo19414.1.exon.9	Spo19414.1.exon.9	exon
Spo19414.1.exon.10	Spo19414.1.exon.10	exon
Spo19414.1.exon.11	Spo19414.1.exon.11	exon

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo19414.1.utr5p.1	Spo19414.1.utr5p.1	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo19414.1.CDS.1	Spo19414.1.CDS.1	CDS
Spo19414.1.CDS.2	Spo19414.1.CDS.2	CDS
Spo19414.1.CDS.3	Spo19414.1.CDS.3	CDS
Spo19414.1.CDS.4	Spo19414.1.CDS.4	CDS
Spo19414.1.CDS.5	Spo19414.1.CDS.5	CDS
Spo19414.1.CDS.6	Spo19414.1.CDS.6	CDS
Spo19414.1.CDS.7	Spo19414.1.CDS.7	CDS
Spo19414.1.CDS.8	Spo19414.1.CDS.8	CDS
Spo19414.1.CDS.9	Spo19414.1.CDS.9	CDS
Spo19414.1.CDS.10	Spo19414.1.CDS.10	CDS
Spo19414.1.CDS.11	Spo19414.1.CDS.11	CDS

Homology

[Close]

BLAST of Spo19414.1 vs. NCBI nr
Match: gi|731317722|ref|XP_010669338.1| (PREDICTED: thermospermine synthase ACAULIS5 isoform X4 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 607.4 bits (1565), Expect = 1.600e-170
Identity = 298/335 (88.96%), Postives = 320/335 (95.52%), Query Frame = 1


		  
Query: 1   MGTEAIEIITSNALSAIFDKQ--NRFNQDNNEFWYEEEIDEDLKWSFALKSVLQKATSEY 60
           MGTEAIEII S AL+AIFD +  + FNQ+ NEFWYEEEIDEDLKWSFAL SVLQKATSEY
Sbjct: 1   MGTEAIEIINSKALTAIFDNKPNSCFNQE-NEFWYEEEIDEDLKWSFALNSVLQKATSEY 60

Query: 61  QDIVLLDTKKFGKVLVLDGKMQSAEADEFIYHECLIHPALLCHLNPKSMFIMGGGEGSAA 120
           QDIVLLDTK+FGKVLVLDGKMQSAEADEFIYHECLIHPALLCH NPK+MFIMGGGEGSAA
Sbjct: 61  QDIVLLDTKRFGKVLVLDGKMQSAEADEFIYHECLIHPALLCHPNPKTMFIMGGGEGSAA 120

Query: 121 REALKHKSIDKVVMCDIDQEVVDFCRKHLTINQEAFNNNKFELVINDAKAELEGRKEKFD 180
           REA KHKSIDKVVMCDIDQEVVDFCRKHLTINQEAFNN K ELVINDAKAEL+GRKEKFD
Sbjct: 121 REAFKHKSIDKVVMCDIDQEVVDFCRKHLTINQEAFNNKKLELVINDAKAELKGRKEKFD 180

Query: 181 IIVGDLADPVEGGPCYQLYTKSFYETILKPRLTDNGIFVTQAGPAGIYTHKEVFSSIYNT 240
           IIVGDLADPVEGGPCYQLYTKSFYE ILKPRLTDNGIF+TQAGPAGIYTHKEVFSSIYNT
Sbjct: 181 IIVGDLADPVEGGPCYQLYTKSFYEKILKPRLTDNGIFITQAGPAGIYTHKEVFSSIYNT 240

Query: 241 IRQVFKYVVAFTAHVPSYADTWGWVMASDHPILIDAKDLDKKIQDRIDGELLYLNGTSIL 300
           IRQVFKYVVAFTAHVPS+ADTWGWVMASDHPILI+A+DLD KIQ+RIDGELLYLNG S+L
Sbjct: 241 IRQVFKYVVAFTAHVPSFADTWGWVMASDHPILINAEDLDDKIQNRIDGELLYLNGASLL 300

Query: 301 SSTSLNKTLSQSLLQETHIYTEESARFLHGHGLNS 334
           SST+LNKTLSQ+L+QETH+YTEE+ARF++GHGL++
Sbjct: 301 SSTTLNKTLSQTLVQETHVYTEENARFIYGHGLSN 334

[Close]

BLAST of Spo19414.1 vs. NCBI nr
Match: gi|731317720|ref|XP_010669337.1| (PREDICTED: thermospermine synthase ACAULIS5 isoform X3 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 602.8 bits (1553), Expect = 4.000e-169
Identity = 298/336 (88.69%), Postives = 319/336 (94.94%), Query Frame = 1


		  
Query: 1   MGTEAIEIITSNALSAIFDKQNR--FNQDNNEFWYEEEIDEDLKWSFALKSVLQKATSEY 60
           MGTEAIEII S AL+AIFD +    FNQ+N EFWYEEEIDEDLKWSFAL SVLQKATSEY
Sbjct: 1   MGTEAIEIINSKALTAIFDNKPNSCFNQEN-EFWYEEEIDEDLKWSFALNSVLQKATSEY 60

Query: 61  QDIVLLDTKKFGKVLVLDGKMQSAEADEFIYHECLIHPALLCHLN-PKSMFIMGGGEGSA 120
           QDIVLLDTK+FGKVLVLDGKMQSAEADEFIYHECLIHPALLCH N PK+MFIMGGGEGSA
Sbjct: 61  QDIVLLDTKRFGKVLVLDGKMQSAEADEFIYHECLIHPALLCHPNSPKTMFIMGGGEGSA 120

Query: 121 AREALKHKSIDKVVMCDIDQEVVDFCRKHLTINQEAFNNNKFELVINDAKAELEGRKEKF 180
           AREA KHKSIDKVVMCDIDQEVVDFCRKHLTINQEAFNN K ELVINDAKAEL+GRKEKF
Sbjct: 121 AREAFKHKSIDKVVMCDIDQEVVDFCRKHLTINQEAFNNKKLELVINDAKAELKGRKEKF 180

Query: 181 DIIVGDLADPVEGGPCYQLYTKSFYETILKPRLTDNGIFVTQAGPAGIYTHKEVFSSIYN 240
           DIIVGDLADPVEGGPCYQLYTKSFYE ILKPRLTDNGIF+TQAGPAGIYTHKEVFSSIYN
Sbjct: 181 DIIVGDLADPVEGGPCYQLYTKSFYEKILKPRLTDNGIFITQAGPAGIYTHKEVFSSIYN 240

Query: 241 TIRQVFKYVVAFTAHVPSYADTWGWVMASDHPILIDAKDLDKKIQDRIDGELLYLNGTSI 300
           TIRQVFKYVVAFTAHVPS+ADTWGWVMASDHPILI+A+DLD KIQ+RIDGELLYLNG S+
Sbjct: 241 TIRQVFKYVVAFTAHVPSFADTWGWVMASDHPILINAEDLDDKIQNRIDGELLYLNGASL 300

Query: 301 LSSTSLNKTLSQSLLQETHIYTEESARFLHGHGLNS 334
           LSST+LNKTLSQ+L+QETH+YTEE+ARF++GHGL++
Sbjct: 301 LSSTTLNKTLSQTLVQETHVYTEENARFIYGHGLSN 335

[Close]

BLAST of Spo19414.1 vs. NCBI nr
Match: gi|224088768|ref|XP_002308531.1| (hypothetical protein POPTR_0006s23880g [Populus trichocarpa])

HSP 1 Score: 541.2 bits (1393), Expect = 1.400e-150
Identity = 256/335 (76.42%), Postives = 297/335 (88.66%), Query Frame = 1


		  
Query: 1   MGTEAIEIITSNALSAIFDKQNRFNQD----NNEFWYEEEIDEDLKWSFALKSVLQKATS 60
           MGTEA+EII +N LS I D  N+ + D    N+  W+EE IDEDLKWSFAL SVL K TS
Sbjct: 1   MGTEAVEIIYANGLSKICDGINQTHSDDQTHNDSSWFEESIDEDLKWSFALNSVLCKVTS 60

Query: 61  EYQDIVLLDTKKFGKVLVLDGKMQSAEADEFIYHECLIHPALLCHLNPKSMFIMGGGEGS 120
           EYQDI LLDTK+FGKVLV+DGKMQSAE DEFIYHECLIHPALLCH NP+++FIMGGGEGS
Sbjct: 61  EYQDITLLDTKRFGKVLVIDGKMQSAEVDEFIYHECLIHPALLCHPNPQNVFIMGGGEGS 120

Query: 121 AAREALKHKSIDKVVMCDIDQEVVDFCRKHLTINQEAFNNNKFELVINDAKAELEGRKEK 180
           AAREALKHKSI+KVVMCDIDQEVVDFCR+HLT+NQEAF+N K +LVINDAKAE+E R EK
Sbjct: 121 AAREALKHKSIEKVVMCDIDQEVVDFCRRHLTVNQEAFSNKKLDLVINDAKAEIEKRNEK 180

Query: 181 FDIIVGDLADPVEGGPCYQLYTKSFYETILKPRLTDNGIFVTQAGPAGIYTHKEVFSSIY 240
           FD+IVGDLADPVEGGPCYQLYTKSFYE ILKP+L DNGIFVTQAGPAG++THKEVFSSIY
Sbjct: 181 FDVIVGDLADPVEGGPCYQLYTKSFYEQILKPKLNDNGIFVTQAGPAGVFTHKEVFSSIY 240

Query: 241 NTIRQVFKYVVAFTAHVPSYADTWGWVMASDHPILIDAKDLDKKIQDRIDGELLYLNGTS 300
           NTI+QVFKYVVA++AHVPS+ADTWGWVMASD P   +A+++D+++ +RIDGELL LNG +
Sbjct: 241 NTIKQVFKYVVAYSAHVPSFADTWGWVMASDQPFCFNAEEIDRRVDERIDGELLLLNGAA 300

Query: 301 ILSSTSLNKTLSQSLLQETHIYTEESARFLHGHGL 332
            LSS +LNK +SQSL+ ETH+YTE++ARFLHGHG+
Sbjct: 301 FLSSATLNKAVSQSLMNETHVYTEDNARFLHGHGV 335

[Close]

BLAST of Spo19414.1 vs. NCBI nr
Match: gi|400268242|gb|AFP74573.1| (thermospermine synthase [Populus tremula x Populus tremuloides])

HSP 1 Score: 540.0 bits (1390), Expect = 3.200e-150
Identity = 256/335 (76.42%), Postives = 296/335 (88.36%), Query Frame = 1


		  
Query: 1   MGTEAIEIITSNALSAIFDKQNRFNQD----NNEFWYEEEIDEDLKWSFALKSVLQKATS 60
           MGTEA+EII +N LS I D  N+   D    N+  W+EE IDEDLKWSFAL SVL K TS
Sbjct: 1   MGTEAVEIIYANGLSKICDGINQTPSDDQTHNDSSWFEESIDEDLKWSFALNSVLCKVTS 60

Query: 61  EYQDIVLLDTKKFGKVLVLDGKMQSAEADEFIYHECLIHPALLCHLNPKSMFIMGGGEGS 120
           EYQDI LLDTK+FGKVLV+DGKMQSAE DEFIYHECLIHPALLCH NP+++FIMGGGEGS
Sbjct: 61  EYQDITLLDTKRFGKVLVIDGKMQSAEVDEFIYHECLIHPALLCHPNPQNVFIMGGGEGS 120

Query: 121 AAREALKHKSIDKVVMCDIDQEVVDFCRKHLTINQEAFNNNKFELVINDAKAELEGRKEK 180
           AAREALKHKSI+KVVMCDIDQEVVDFCR+HLT+NQEAF+N K +LVINDAKAE+E R EK
Sbjct: 121 AAREALKHKSIEKVVMCDIDQEVVDFCRRHLTVNQEAFSNKKLDLVINDAKAEIEKRNEK 180

Query: 181 FDIIVGDLADPVEGGPCYQLYTKSFYETILKPRLTDNGIFVTQAGPAGIYTHKEVFSSIY 240
           FD+IVGDLADPVEGGPCYQLYTKSFYE ILKP+L DNGIFVTQAGPAG++THKEVFSSIY
Sbjct: 181 FDVIVGDLADPVEGGPCYQLYTKSFYEQILKPKLNDNGIFVTQAGPAGVFTHKEVFSSIY 240

Query: 241 NTIRQVFKYVVAFTAHVPSYADTWGWVMASDHPILIDAKDLDKKIQDRIDGELLYLNGTS 300
           NTI+QVFKYVVA++AHVPS+ADTWGWVMASD P   +A+++D+++ +RIDGELL LNG +
Sbjct: 241 NTIKQVFKYVVAYSAHVPSFADTWGWVMASDQPFCFNAEEIDRRVDERIDGELLLLNGAA 300

Query: 301 ILSSTSLNKTLSQSLLQETHIYTEESARFLHGHGL 332
            LSS +LNK +SQSL+ ETH+YTE++ARFLHGHG+
Sbjct: 301 FLSSATLNKAVSQSLMNETHVYTEDNARFLHGHGV 335

[Close]

BLAST of Spo19414.1 vs. NCBI nr
Match: gi|743812837|ref|XP_011019369.1| (PREDICTED: thermospermine synthase ACAULIS5 [Populus euphratica])

HSP 1 Score: 538.5 bits (1386), Expect = 9.400e-150
Identity = 254/335 (75.82%), Postives = 297/335 (88.66%), Query Frame = 1


		  
Query: 1   MGTEAIEIITSNALSAIFDKQNRFNQD----NNEFWYEEEIDEDLKWSFALKSVLQKATS 60
           MGTEA+EII +N LS I D  N+ + D    N+  W+EE IDEDLKWSFA+ SVL K TS
Sbjct: 1   MGTEAVEIIYANGLSKICDGINQTHSDDQTHNDSSWFEESIDEDLKWSFAVNSVLCKVTS 60

Query: 61  EYQDIVLLDTKKFGKVLVLDGKMQSAEADEFIYHECLIHPALLCHLNPKSMFIMGGGEGS 120
           EYQDI LLDTK+FGKVLV+DGKMQSAE DEFIYHECLIHPALLCH NP+++FIMGGGEGS
Sbjct: 61  EYQDITLLDTKRFGKVLVIDGKMQSAEVDEFIYHECLIHPALLCHPNPQNVFIMGGGEGS 120

Query: 121 AAREALKHKSIDKVVMCDIDQEVVDFCRKHLTINQEAFNNNKFELVINDAKAELEGRKEK 180
           AAREALKHKSI+KVVMCDIDQEVVDFCR+HLT+NQEAF+N K +LVINDAKAE+E R EK
Sbjct: 121 AAREALKHKSIEKVVMCDIDQEVVDFCRRHLTVNQEAFSNKKLDLVINDAKAEIEKRNEK 180

Query: 181 FDIIVGDLADPVEGGPCYQLYTKSFYETILKPRLTDNGIFVTQAGPAGIYTHKEVFSSIY 240
           FD+IVGDLADPVEGGPCYQLYTKSFYE ILKP+L DNGIFVTQAGPAG++THKEVFSSI+
Sbjct: 181 FDVIVGDLADPVEGGPCYQLYTKSFYEQILKPKLNDNGIFVTQAGPAGVFTHKEVFSSIF 240

Query: 241 NTIRQVFKYVVAFTAHVPSYADTWGWVMASDHPILIDAKDLDKKIQDRIDGELLYLNGTS 300
           NTI+QVFKYVVA++AHVPS+ADTWGWVMASD P   +A+++D+++ +RIDGELL LNG +
Sbjct: 241 NTIKQVFKYVVAYSAHVPSFADTWGWVMASDQPFCFNAEEIDRRVDERIDGELLLLNGAA 300

Query: 301 ILSSTSLNKTLSQSLLQETHIYTEESARFLHGHGL 332
            LSS +LNK +SQSL+ ETH+YTE++ARFLHGHG+
Sbjct: 301 FLSSATLNKAVSQSLMNETHVYTEDNARFLHGHGV 335

[Close]

BLAST of Spo19414.1 vs. UniProtKB/TrEMBL
Match: A0A0J8CW98_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_2g034040 PE=3 SV=1)

HSP 1 Score: 607.4 bits (1565), Expect = 1.100e-170
Identity = 298/335 (88.96%), Postives = 320/335 (95.52%), Query Frame = 1


		  
Query: 1   MGTEAIEIITSNALSAIFDKQ--NRFNQDNNEFWYEEEIDEDLKWSFALKSVLQKATSEY 60
           MGTEAIEII S AL+AIFD +  + FNQ+ NEFWYEEEIDEDLKWSFAL SVLQKATSEY
Sbjct: 1   MGTEAIEIINSKALTAIFDNKPNSCFNQE-NEFWYEEEIDEDLKWSFALNSVLQKATSEY 60

Query: 61  QDIVLLDTKKFGKVLVLDGKMQSAEADEFIYHECLIHPALLCHLNPKSMFIMGGGEGSAA 120
           QDIVLLDTK+FGKVLVLDGKMQSAEADEFIYHECLIHPALLCH NPK+MFIMGGGEGSAA
Sbjct: 61  QDIVLLDTKRFGKVLVLDGKMQSAEADEFIYHECLIHPALLCHPNPKTMFIMGGGEGSAA 120

Query: 121 REALKHKSIDKVVMCDIDQEVVDFCRKHLTINQEAFNNNKFELVINDAKAELEGRKEKFD 180
           REA KHKSIDKVVMCDIDQEVVDFCRKHLTINQEAFNN K ELVINDAKAEL+GRKEKFD
Sbjct: 121 REAFKHKSIDKVVMCDIDQEVVDFCRKHLTINQEAFNNKKLELVINDAKAELKGRKEKFD 180

Query: 181 IIVGDLADPVEGGPCYQLYTKSFYETILKPRLTDNGIFVTQAGPAGIYTHKEVFSSIYNT 240
           IIVGDLADPVEGGPCYQLYTKSFYE ILKPRLTDNGIF+TQAGPAGIYTHKEVFSSIYNT
Sbjct: 181 IIVGDLADPVEGGPCYQLYTKSFYEKILKPRLTDNGIFITQAGPAGIYTHKEVFSSIYNT 240

Query: 241 IRQVFKYVVAFTAHVPSYADTWGWVMASDHPILIDAKDLDKKIQDRIDGELLYLNGTSIL 300
           IRQVFKYVVAFTAHVPS+ADTWGWVMASDHPILI+A+DLD KIQ+RIDGELLYLNG S+L
Sbjct: 241 IRQVFKYVVAFTAHVPSFADTWGWVMASDHPILINAEDLDDKIQNRIDGELLYLNGASLL 300

Query: 301 SSTSLNKTLSQSLLQETHIYTEESARFLHGHGLNS 334
           SST+LNKTLSQ+L+QETH+YTEE+ARF++GHGL++
Sbjct: 301 SSTTLNKTLSQTLVQETHVYTEENARFIYGHGLSN 334

[Close]

BLAST of Spo19414.1 vs. UniProtKB/TrEMBL
Match: A0A0J8D134_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_2g034040 PE=3 SV=1)

HSP 1 Score: 602.8 bits (1553), Expect = 2.800e-169
Identity = 298/336 (88.69%), Postives = 319/336 (94.94%), Query Frame = 1


		  
Query: 1   MGTEAIEIITSNALSAIFDKQNR--FNQDNNEFWYEEEIDEDLKWSFALKSVLQKATSEY 60
           MGTEAIEII S AL+AIFD +    FNQ+N EFWYEEEIDEDLKWSFAL SVLQKATSEY
Sbjct: 1   MGTEAIEIINSKALTAIFDNKPNSCFNQEN-EFWYEEEIDEDLKWSFALNSVLQKATSEY 60

Query: 61  QDIVLLDTKKFGKVLVLDGKMQSAEADEFIYHECLIHPALLCHLN-PKSMFIMGGGEGSA 120
           QDIVLLDTK+FGKVLVLDGKMQSAEADEFIYHECLIHPALLCH N PK+MFIMGGGEGSA
Sbjct: 61  QDIVLLDTKRFGKVLVLDGKMQSAEADEFIYHECLIHPALLCHPNSPKTMFIMGGGEGSA 120

Query: 121 AREALKHKSIDKVVMCDIDQEVVDFCRKHLTINQEAFNNNKFELVINDAKAELEGRKEKF 180
           AREA KHKSIDKVVMCDIDQEVVDFCRKHLTINQEAFNN K ELVINDAKAEL+GRKEKF
Sbjct: 121 AREAFKHKSIDKVVMCDIDQEVVDFCRKHLTINQEAFNNKKLELVINDAKAELKGRKEKF 180

Query: 181 DIIVGDLADPVEGGPCYQLYTKSFYETILKPRLTDNGIFVTQAGPAGIYTHKEVFSSIYN 240
           DIIVGDLADPVEGGPCYQLYTKSFYE ILKPRLTDNGIF+TQAGPAGIYTHKEVFSSIYN
Sbjct: 181 DIIVGDLADPVEGGPCYQLYTKSFYEKILKPRLTDNGIFITQAGPAGIYTHKEVFSSIYN 240

Query: 241 TIRQVFKYVVAFTAHVPSYADTWGWVMASDHPILIDAKDLDKKIQDRIDGELLYLNGTSI 300
           TIRQVFKYVVAFTAHVPS+ADTWGWVMASDHPILI+A+DLD KIQ+RIDGELLYLNG S+
Sbjct: 241 TIRQVFKYVVAFTAHVPSFADTWGWVMASDHPILINAEDLDDKIQNRIDGELLYLNGASL 300

Query: 301 LSSTSLNKTLSQSLLQETHIYTEESARFLHGHGLNS 334
           LSST+LNKTLSQ+L+QETH+YTEE+ARF++GHGL++
Sbjct: 301 LSSTTLNKTLSQTLVQETHVYTEENARFIYGHGLSN 335

[Close]

BLAST of Spo19414.1 vs. UniProtKB/TrEMBL
Match: B9HD20_POPTR (Uncharacterized protein OS=Populus trichocarpa GN=POPTR_0006s23880g PE=3 SV=1)

HSP 1 Score: 541.2 bits (1393), Expect = 1.000e-150
Identity = 256/335 (76.42%), Postives = 297/335 (88.66%), Query Frame = 1


		  
Query: 1   MGTEAIEIITSNALSAIFDKQNRFNQD----NNEFWYEEEIDEDLKWSFALKSVLQKATS 60
           MGTEA+EII +N LS I D  N+ + D    N+  W+EE IDEDLKWSFAL SVL K TS
Sbjct: 1   MGTEAVEIIYANGLSKICDGINQTHSDDQTHNDSSWFEESIDEDLKWSFALNSVLCKVTS 60

Query: 61  EYQDIVLLDTKKFGKVLVLDGKMQSAEADEFIYHECLIHPALLCHLNPKSMFIMGGGEGS 120
           EYQDI LLDTK+FGKVLV+DGKMQSAE DEFIYHECLIHPALLCH NP+++FIMGGGEGS
Sbjct: 61  EYQDITLLDTKRFGKVLVIDGKMQSAEVDEFIYHECLIHPALLCHPNPQNVFIMGGGEGS 120

Query: 121 AAREALKHKSIDKVVMCDIDQEVVDFCRKHLTINQEAFNNNKFELVINDAKAELEGRKEK 180
           AAREALKHKSI+KVVMCDIDQEVVDFCR+HLT+NQEAF+N K +LVINDAKAE+E R EK
Sbjct: 121 AAREALKHKSIEKVVMCDIDQEVVDFCRRHLTVNQEAFSNKKLDLVINDAKAEIEKRNEK 180

Query: 181 FDIIVGDLADPVEGGPCYQLYTKSFYETILKPRLTDNGIFVTQAGPAGIYTHKEVFSSIY 240
           FD+IVGDLADPVEGGPCYQLYTKSFYE ILKP+L DNGIFVTQAGPAG++THKEVFSSIY
Sbjct: 181 FDVIVGDLADPVEGGPCYQLYTKSFYEQILKPKLNDNGIFVTQAGPAGVFTHKEVFSSIY 240

Query: 241 NTIRQVFKYVVAFTAHVPSYADTWGWVMASDHPILIDAKDLDKKIQDRIDGELLYLNGTS 300
           NTI+QVFKYVVA++AHVPS+ADTWGWVMASD P   +A+++D+++ +RIDGELL LNG +
Sbjct: 241 NTIKQVFKYVVAYSAHVPSFADTWGWVMASDQPFCFNAEEIDRRVDERIDGELLLLNGAA 300

Query: 301 ILSSTSLNKTLSQSLLQETHIYTEESARFLHGHGL 332
            LSS +LNK +SQSL+ ETH+YTE++ARFLHGHG+
Sbjct: 301 FLSSATLNKAVSQSLMNETHVYTEDNARFLHGHGV 335

[Close]

BLAST of Spo19414.1 vs. UniProtKB/TrEMBL
Match: T1PPE6_9ROSI (Thermospermine synthase OS=Populus tremula x Populus tremuloides GN=POPACAULIS5 PE=2 SV=1)

HSP 1 Score: 540.0 bits (1390), Expect = 2.200e-150
Identity = 256/335 (76.42%), Postives = 296/335 (88.36%), Query Frame = 1


		  
Query: 1   MGTEAIEIITSNALSAIFDKQNRFNQD----NNEFWYEEEIDEDLKWSFALKSVLQKATS 60
           MGTEA+EII +N LS I D  N+   D    N+  W+EE IDEDLKWSFAL SVL K TS
Sbjct: 1   MGTEAVEIIYANGLSKICDGINQTPSDDQTHNDSSWFEESIDEDLKWSFALNSVLCKVTS 60

Query: 61  EYQDIVLLDTKKFGKVLVLDGKMQSAEADEFIYHECLIHPALLCHLNPKSMFIMGGGEGS 120
           EYQDI LLDTK+FGKVLV+DGKMQSAE DEFIYHECLIHPALLCH NP+++FIMGGGEGS
Sbjct: 61  EYQDITLLDTKRFGKVLVIDGKMQSAEVDEFIYHECLIHPALLCHPNPQNVFIMGGGEGS 120

Query: 121 AAREALKHKSIDKVVMCDIDQEVVDFCRKHLTINQEAFNNNKFELVINDAKAELEGRKEK 180
           AAREALKHKSI+KVVMCDIDQEVVDFCR+HLT+NQEAF+N K +LVINDAKAE+E R EK
Sbjct: 121 AAREALKHKSIEKVVMCDIDQEVVDFCRRHLTVNQEAFSNKKLDLVINDAKAEIEKRNEK 180

Query: 181 FDIIVGDLADPVEGGPCYQLYTKSFYETILKPRLTDNGIFVTQAGPAGIYTHKEVFSSIY 240
           FD+IVGDLADPVEGGPCYQLYTKSFYE ILKP+L DNGIFVTQAGPAG++THKEVFSSIY
Sbjct: 181 FDVIVGDLADPVEGGPCYQLYTKSFYEQILKPKLNDNGIFVTQAGPAGVFTHKEVFSSIY 240

Query: 241 NTIRQVFKYVVAFTAHVPSYADTWGWVMASDHPILIDAKDLDKKIQDRIDGELLYLNGTS 300
           NTI+QVFKYVVA++AHVPS+ADTWGWVMASD P   +A+++D+++ +RIDGELL LNG +
Sbjct: 241 NTIKQVFKYVVAYSAHVPSFADTWGWVMASDQPFCFNAEEIDRRVDERIDGELLLLNGAA 300

Query: 301 ILSSTSLNKTLSQSLLQETHIYTEESARFLHGHGL 332
            LSS +LNK +SQSL+ ETH+YTE++ARFLHGHG+
Sbjct: 301 FLSSATLNKAVSQSLMNETHVYTEDNARFLHGHGV 335

[Close]

BLAST of Spo19414.1 vs. UniProtKB/TrEMBL
Match: A0A067KD25_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_07613 PE=3 SV=1)

HSP 1 Score: 525.0 bits (1351), Expect = 7.500e-146
Identity = 250/332 (75.30%), Postives = 290/332 (87.35%), Query Frame = 1


		  
Query: 4   EAIEIITSNALSAIFDKQNRFNQD----NNEFWYEEEIDEDLKWSFALKSVLQKATSEYQ 63
           EA+EII +N  S + +     N +    ++  WYEE ID+DLKWSFAL SVL K TSE+Q
Sbjct: 3   EAVEIIFTNGFSKLCNDTTTNNNNIISLDDSSWYEETIDDDLKWSFALNSVLHKGTSEFQ 62

Query: 64  DIVLLDTKKFGKVLVLDGKMQSAEADEFIYHECLIHPALLCHLNPKSMFIMGGGEGSAAR 123
           DI LLDTK+FGKVLV+DGKMQSAEADEFIYHECLIHPA LCH  PK +FIMGGGEGSAAR
Sbjct: 63  DIALLDTKRFGKVLVIDGKMQSAEADEFIYHECLIHPAFLCHPKPKKVFIMGGGEGSAAR 122

Query: 124 EALKHKSIDKVVMCDIDQEVVDFCRKHLTINQEAFNNNKFELVINDAKAELEGRKEKFDI 183
           EALKHKSI+KVVMCDIDQEVVDFCR+HLT+NQ+AF + K  LVINDAKAELE R +KFDI
Sbjct: 123 EALKHKSIEKVVMCDIDQEVVDFCRRHLTVNQQAFCHKKLNLVINDAKAELEKRNDKFDI 182

Query: 184 IVGDLADPVEGGPCYQLYTKSFYETILKPRLTDNGIFVTQAGPAGIYTHKEVFSSIYNTI 243
           I+GDLADPVEGGPCYQLYTKSFY+ ILKP+L+DNGIFVTQAGPAGI+THKEVFSSIYNTI
Sbjct: 183 IIGDLADPVEGGPCYQLYTKSFYQRILKPKLSDNGIFVTQAGPAGIFTHKEVFSSIYNTI 242

Query: 244 RQVFKYVVAFTAHVPSYADTWGWVMASDHPILIDAKDLDKKIQDRIDGELLYLNGTSILS 303
           +QVFKYVVA++AHVPS+ADTWGWVMASD P  IDA+++D++I++RI GELLYLNG + LS
Sbjct: 243 KQVFKYVVAYSAHVPSFADTWGWVMASDQPFCIDAEEIDRRIEERIQGELLYLNGAAFLS 302

Query: 304 STSLNKTLSQSLLQETHIYTEESARFLHGHGL 332
           ST+LNKT+SQSLL ETH+YTE+ ARF+ GHGL
Sbjct: 303 STTLNKTVSQSLLNETHVYTEDDARFIPGHGL 334

[Close]

BLAST of Spo19414.1 vs. ExPASy Swiss-Prot
Match: ACL5_ARATH (Thermospermine synthase ACAULIS5 OS=Arabidopsis thaliana GN=ACL5 PE=1 SV=1)

HSP 1 Score: 487.6 bits (1254), Expect = 1.200e-136
Identity = 231/332 (69.58%), Postives = 279/332 (84.04%), Query Frame = 1


		  
Query: 4   EAIEIITSNALSAIFDK----QNRFNQDNNEFWYEEEIDEDLKWSFALKSVLQKATSEYQ 63
           EA+E++  N    I       Q   +   +  WYEE ID+DLKWSFAL SVL + TSEYQ
Sbjct: 3   EAVEVMFGNGFPEIHKATSPTQTLHSNQQDCHWYEETIDDDLKWSFALNSVLHQGTSEYQ 62

Query: 64  DIVLLDTKKFGKVLVLDGKMQSAEADEFIYHECLIHPALLCHLNPKSMFIMGGGEGSAAR 123
           DI LLDTK+FGKVLV+DGKMQSAE DEFIYHECLIHPALL H NPK++FIMGGGEGSAAR
Sbjct: 63  DIALLDTKRFGKVLVIDGKMQSAERDEFIYHECLIHPALLFHPNPKTVFIMGGGEGSAAR 122

Query: 124 EALKHKSIDKVVMCDIDQEVVDFCRKHLTINQEAFNNNKFELVINDAKAELEGRKEKFDI 183
           E LKH +I+KVVMCDIDQEVVDFCR+ LT+N +AF N K ELVI DAKAELE R+EKFDI
Sbjct: 123 EILKHTTIEKVVMCDIDQEVVDFCRRFLTVNSDAFCNKKLELVIKDAKAELEKREEKFDI 182

Query: 184 IVGDLADPVEGGPCYQLYTKSFYETILKPRLTDNGIFVTQAGPAGIYTHKEVFSSIYNTI 243
           IVGDLADPVEGGPCYQLYTKSFY+ ILKP+L+ NGIFVTQAGPAGI+THKEVF+SIYNT+
Sbjct: 183 IVGDLADPVEGGPCYQLYTKSFYQNILKPKLSPNGIFVTQAGPAGIFTHKEVFTSIYNTM 242

Query: 244 RQVFKYVVAFTAHVPSYADTWGWVMASDHPILIDAKDLDKKIQDRIDGELLYLNGTSILS 303
           +QVFKYV A+TAHVPS+ADTWGWVMASDH   ++  ++D++I++R++GEL+YLN  S +S
Sbjct: 243 KQVFKYVKAYTAHVPSFADTWGWVMASDHEFDVEVDEMDRRIEERVNGELMYLNAPSFVS 302

Query: 304 STSLNKTLSQSLLQETHIYTEESARFLHGHGL 332
           + +LNKT+S +L +ET +Y+EE+ARF+HGHG+
Sbjct: 303 AATLNKTISLALEKETEVYSEENARFIHGHGV 334

[Close]

BLAST of Spo19414.1 vs. ExPASy Swiss-Prot
Match: SPEE_SULTO (Polyamine aminopropyltransferase OS=Sulfolobus tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) GN=speE PE=3 SV=1)

HSP 1 Score: 219.9 bits (559), Expect = 4.600e-56
Identity = 119/280 (42.50%), Postives = 173/280 (61.79%), Query Frame = 1


		  
Query: 46  ALKSVLQKATSEYQDIVLLDTKKFGKVLVLDGKMQSAEADEFIYHECLIHPALLCHLNPK 105
           A+  V+ +  + YQ  +L++  +FGK L+LDGK+QS   DEFIYHE L+HP LL   NP+
Sbjct: 20  AITKVIAEEKTPYQRALLVELARFGKALILDGKIQSTITDEFIYHEALVHPLLLSINNPE 79

Query: 106 SMFIMGGGEGSAAREALKHKSIDKVVMCDIDQEVVDFCRKHL-TINQEAFNNNKFELVIN 165
            + I+GGGEG+  RE LKHK+I  V M DID  V+DF +K+L   +Q AF+N K +LVI 
Sbjct: 80  KILILGGGEGATLREVLKHKTIKNVTMVDIDPVVIDFAKKYLQEWHQGAFDNPKSKLVIE 139

Query: 166 DAKAELEGRKEKFDIIVGDLADPVEGGPCYQLYTKSFYETILKPRLTDNGIFVTQAGPAG 225
           D    ++  KEKFD +V DL DP++  P   LYTK FYE +   R++  GI      P+ 
Sbjct: 140 DGYKFIKETKEKFDAVVIDLTDPIKDSPSQMLYTKEFYEEV--KRISKWGIVTQATSPS- 199

Query: 226 IYTHKEVFSSIYNTIRQVFKYVVAFTAHVPSYADTWGWVMASD--HPILIDAKDLDKKIQ 285
                E FS IYNTI+ VFK V A   +VP++   WG+V ASD  +P     ++++ +I+
Sbjct: 200 --FSLETFSIIYNTIKHVFKKVSAGITYVPAFDGLWGFVYASDEVNPAEFSKEEINNRIK 259

Query: 286 DRIDGELLYLNGTSILSSTSLNKTLSQSLLQETHIYTEES 323
           +RIDG L + +G + ++  S+ K + + L +E  I T E+
Sbjct: 260 ERIDGSLRFYDGETHITMFSIPKHIREVLEKENKISTREN 294

[Close]

BLAST of Spo19414.1 vs. ExPASy Swiss-Prot
Match: SPEE_SULSO (Polyamine aminopropyltransferase OS=Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) GN=speE PE=1 SV=1)

HSP 1 Score: 212.2 bits (539), Expect = 9.600e-54
Identity = 116/277 (41.88%), Postives = 169/277 (61.01%), Query Frame = 1


		  
Query: 47  LKSVLQKATSEYQDIVLLDTKKFGKVLVLDGKMQSAEADEFIYHECLIHPALLCHLNP-K 106
           ++ VL +  + YQ + L++  +FGK L++DGK+QS   DE IYHE L+HP LL    P K
Sbjct: 21  IEKVLAEEKTPYQHVTLVEFTRFGKGLIIDGKVQSTLYDEHIYHELLVHPLLLSLTKPPK 80

Query: 107 SMFIMGGGEGSAAREALKHKSIDKVVMCDIDQEVVDFCRKHL-TINQEAFNNNKFELVIN 166
           S+ I+GGGEG+  RE LK+KS++K VM DID++V++F +K+L   +Q AF + +  LVI 
Sbjct: 81  SVLILGGGEGATLREVLKYKSVEKAVMVDIDEKVIEFAKKYLYEWHQGAFEDKRTNLVIT 140

Query: 167 DAKAELEGRKEKFDIIVGDLADPVEGGPCYQLYTKSFYETILKPRLTDNGIFVTQAGPAG 226
           D    ++  KEK+D I+ DL DP++    Y LYTK FYE  L+  L + G  VTQA    
Sbjct: 141 DGLKFIKETKEKYDAIILDLTDPIKNSTSYMLYTKEFYEK-LREILNERGGIVTQATSPS 200

Query: 227 IYTHKEVFSSIYNTIRQVFKYVVAFTAHVPSYADTWGWVMASDHPILIDAKDLDKKIQDR 286
                EV+ +IYNTI++VFK   A   ++ S+   WG+V     P L+   ++D KI +R
Sbjct: 201 F--SLEVYVTIYNTIKEVFKEASASYTYMASFDGLWGFVYGGVRPDLLSEDEVDSKINER 260

Query: 287 IDGELLYLNGTSILSSTSLNKTLSQSLLQETHIYTEE 322
           IDG+L + +G S   S SL K +     + T I TE+
Sbjct: 261 IDGQLRFYDGYSHKISFSLPKNIKSEFQKITKISTEK 294

[Close]

BLAST of Spo19414.1 vs. ExPASy Swiss-Prot
Match: SPEE_AERPE (Polyamine aminopropyltransferase OS=Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1) GN=speE PE=3 SV=2)

HSP 1 Score: 204.9 bits (520), Expect = 1.500e-51
Identity = 113/301 (37.54%), Postives = 187/301 (62.13%), Query Frame = 1


		  
Query: 32  WYEEEIDEDLKWSFALKSVLQKATSEYQDIVLLDTKKFGKVLVLDGKMQSAEADEFIYHE 91
           W+ E +  D      ++ V+ +  S +Q+I ++     GKVLVLDGK QS+E+DEF+YHE
Sbjct: 15  WFLEWLTPDRATLKHIEDVIFQGRSRFQEIAVVRVSGEGKVLVLDGKTQSSESDEFMYHE 74

Query: 92  CLIHPALLCHLNPKSMFIMGGGEGSAAREALKHKSIDKVVMCDIDQEVVDFCRKHL-TIN 151
            L+HPA++ H +P+ + I+GGGEG+  RE LKH+S++K VM DID+ VV+  R+HL   +
Sbjct: 75  ALVHPAMILHGSPRKVLILGGGEGATLREVLKHRSVEKAVMVDIDETVVNVAREHLREWH 134

Query: 152 QEAFNNNKFELVINDAKAELEGRKEK-FDIIVGDLADPVEGGPCYQLYTKSFYETILKPR 211
           + AF++ + E+VI+DA   +  + E  FD+++ DL DP+E GP  +LY++ +Y  ++K  
Sbjct: 135 RGAFDDPRAEVVIDDAWNYVASKAETGFDVVIADLVDPLEAGPATRLYSEEYYR-MVKDV 194

Query: 212 LTDNGIFVTQAGPAGIYTHKEVFSSIYNTIRQVFKYVVAFTAHVPSYADTWGWVMASDHP 271
           +   G+FVTQA    I    E  + I NT+ +VFK+V ++  +VPS+   WG+V+ASD  
Sbjct: 195 MNPGGVFVTQA--VSISHLTEYHAIIRNTVARVFKHVESYGVYVPSFDSMWGFVVASDDK 254

Query: 272 ---ILIDAKDLDKKIQDRIDG-ELLYLNGTSILSSTSLNKTLSQSLLQETHIYTEESARF 327
              IL D    + ++  ++ G EL +L+  S+L   ++ K   +++ +E    T ++  F
Sbjct: 255 DPRILGDRGFFETRLSHQLQGAELRFLDYASMLHMLNIPKMYREAIAREKRYATLDNQVF 312

[Close]

BLAST of Spo19414.1 vs. ExPASy Swiss-Prot
Match: SPEE_SULIM (Polyamine aminopropyltransferase OS=Sulfolobus islandicus (strain M.14.25 / Kamchatka #1) GN=speE PE=3 SV=1)

HSP 1 Score: 197.6 bits (501), Expect = 2.500e-49
Identity = 108/277 (38.99%), Postives = 165/277 (59.57%), Query Frame = 1


		  
Query: 47  LKSVLQKATSEYQDIVLLDTKKFGKVLVLDGKMQSAEADEFIYHECLIHPALLCHLNP-K 106
           ++ V  +  + YQ + L++  +FGK L++DGK+QS   DE IYHE L+HP LL    P K
Sbjct: 21  IEKVFAEEKTPYQHVTLVEFTRFGKGLIIDGKVQSTLYDEHIYHELLVHPLLLSLPKPPK 80

Query: 107 SMFIMGGGEGSAAREALKHKSIDKVVMCDIDQEVVDFCRKHL-TINQEAFNNNKFELVIN 166
           ++ I+GGGEG+  RE LK+KS++K VM DID++V++F +K+L   +Q AF + +  LVI 
Sbjct: 81  NVLILGGGEGATLREVLKYKSVEKAVMVDIDEKVIEFAKKYLYEWHQGAFEDKRTSLVIT 140

Query: 167 DAKAELEGRKEKFDIIVGDLADPVEGGPCYQLYTKSFYETILKPRLTDNGIFVTQAGPAG 226
           D    +   K+K+D I+ DL DP++    Y LYTK FYE  L+  L   G  VTQA    
Sbjct: 141 DGLKFINETKDKYDAIILDLTDPIKDSTSYMLYTKEFYEK-LRGILNQGGGIVTQATSPS 200

Query: 227 IYTHKEVFSSIYNTIRQVFKYVVAFTAHVPSYADTWGWVMASDHPILIDAKDLDKKIQDR 286
                EV+ +IYNTI++VFK   A   ++ S+   WG+V     P L+   +++ +I++R
Sbjct: 201 F--SLEVYVTIYNTIKEVFKEASASYTYMASFDGLWGFVYGGVRPDLLSEDEVNSRIRER 260

Query: 287 IDGELLYLNGTSILSSTSLNKTLSQSLLQETHIYTEE 322
           I G+L + +  S   S SL K +     + T + TE+
Sbjct: 261 ISGQLRFYDDYSHKISFSLPKNIKSEFKKITKVSTEK 294

[Close]

BLAST of Spo19414.1 vs. TAIR (Arabidopsis)
Match: AT5G19530.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein)

HSP 1 Score: 487.6 bits (1254), Expect = 6.700e-138
Identity = 231/332 (69.58%), Postives = 279/332 (84.04%), Query Frame = 1


		  
Query: 4   EAIEIITSNALSAIFDK----QNRFNQDNNEFWYEEEIDEDLKWSFALKSVLQKATSEYQ 63
           EA+E++  N    I       Q   +   +  WYEE ID+DLKWSFAL SVL + TSEYQ
Sbjct: 3   EAVEVMFGNGFPEIHKATSPTQTLHSNQQDCHWYEETIDDDLKWSFALNSVLHQGTSEYQ 62

Query: 64  DIVLLDTKKFGKVLVLDGKMQSAEADEFIYHECLIHPALLCHLNPKSMFIMGGGEGSAAR 123
           DI LLDTK+FGKVLV+DGKMQSAE DEFIYHECLIHPALL H NPK++FIMGGGEGSAAR
Sbjct: 63  DIALLDTKRFGKVLVIDGKMQSAERDEFIYHECLIHPALLFHPNPKTVFIMGGGEGSAAR 122

Query: 124 EALKHKSIDKVVMCDIDQEVVDFCRKHLTINQEAFNNNKFELVINDAKAELEGRKEKFDI 183
           E LKH +I+KVVMCDIDQEVVDFCR+ LT+N +AF N K ELVI DAKAELE R+EKFDI
Sbjct: 123 EILKHTTIEKVVMCDIDQEVVDFCRRFLTVNSDAFCNKKLELVIKDAKAELEKREEKFDI 182

Query: 184 IVGDLADPVEGGPCYQLYTKSFYETILKPRLTDNGIFVTQAGPAGIYTHKEVFSSIYNTI 243
           IVGDLADPVEGGPCYQLYTKSFY+ ILKP+L+ NGIFVTQAGPAGI+THKEVF+SIYNT+
Sbjct: 183 IVGDLADPVEGGPCYQLYTKSFYQNILKPKLSPNGIFVTQAGPAGIFTHKEVFTSIYNTM 242

Query: 244 RQVFKYVVAFTAHVPSYADTWGWVMASDHPILIDAKDLDKKIQDRIDGELLYLNGTSILS 303
           +QVFKYV A+TAHVPS+ADTWGWVMASDH   ++  ++D++I++R++GEL+YLN  S +S
Sbjct: 243 KQVFKYVKAYTAHVPSFADTWGWVMASDHEFDVEVDEMDRRIEERVNGELMYLNAPSFVS 302

Query: 304 STSLNKTLSQSLLQETHIYTEESARFLHGHGL 332
           + +LNKT+S +L +ET +Y+EE+ARF+HGHG+
Sbjct: 303 AATLNKTISLALEKETEVYSEENARFIHGHGV 334

[Close]

BLAST of Spo19414.1 vs. TAIR (Arabidopsis)
Match: AT1G70310.1 (spermidine synthase 2)

HSP 1 Score: 137.1 bits (344), Expect = 2.200e-32
Identity = 73/235 (31.06%), Postives = 130/235 (55.32%), Query Frame = 1


		  
Query: 44  SFALKSVLQKATSEYQDIVLLDTKKFGKVLVLDGKMQSAEADEFIYHECLIHPALLCHLN 103
           S  ++ +L +  S+YQD+++  +  +GKVLVLDG +Q  E DE  Y E + H  L    N
Sbjct: 65  SLKVEKILFQGKSDYQDVIVFQSATYGKVLVLDGVIQLTERDECAYQEMITHLPLCSISN 124

Query: 104 PKSMFIMGGGEGSAAREALKHKSIDKVVMCDIDQEVVDFCRKHLTINQEAFNNNKFELVI 163
           PK + ++GGG+G   RE  +H S++++ +C+ID+ VVD  +++       + + +  L+I
Sbjct: 125 PKKVLVIGGGDGGVLREVARHSSVEQIDICEIDKMVVDVAKQYFPNVAVGYEDPRVNLII 184

Query: 164 NDAKAELEGRKE-KFDIIVGDLADPVEGGPCYQLYTKSFYETILKPRLTDNGIFVTQAGP 223
            D  A L+   E  +D ++ D +DP+  GP  +L+ K F+E++ +  L   G+  TQA  
Sbjct: 185 GDGVAFLKNAAEGTYDAVIVDSSDPI--GPAKELFEKPFFESVNR-ALRPGGVVCTQA-- 244

Query: 224 AGIYTHKEVFSSIYNTIRQVFKYVVAFT-AHVPSY-ADTWGWVMASDHPILIDAK 276
             ++ H ++   I +  R +FK  V +    VP+Y +   G+++ S     +D K
Sbjct: 245 ESLWLHMDIIEDIVSNCRDIFKGSVNYAWTSVPTYPSGVIGFMLCSSEGPQVDFK 294

[Close]

BLAST of Spo19414.1 vs. TAIR (Arabidopsis)
Match: AT1G23820.1 (spermidine synthase 1)

HSP 1 Score: 136.7 bits (343), Expect = 2.900e-32
Identity = 76/235 (32.34%), Postives = 130/235 (55.32%), Query Frame = 1


		  
Query: 44  SFALKSVLQKATSEYQDIVLLDTKKFGKVLVLDGKMQSAEADEFIYHECLIHPALLCHLN 103
           S  ++ VL +  S+YQD+++  +  +GKVLVLDG +Q  E DE  Y E + H  L    N
Sbjct: 105 SLKVEKVLFQGKSDYQDVIVFQSATYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPN 164

Query: 104 PKSMFIMGGGEGSAAREALKHKSIDKVVMCDIDQEVVDFCRKHLTINQEAFNNNKFELVI 163
           PK + ++GGG+G   RE  +H SI+++ MC+ID+ VVD  ++        + + +  LVI
Sbjct: 165 PKKVLVIGGGDGGVLREVARHASIEQIDMCEIDKMVVDVSKQFFPDVAIGYEDPRVNLVI 224

Query: 164 NDAKAELEGRKE-KFDIIVGDLADPVEGGPCYQLYTKSFYETILKPRLTDNGIFVTQAGP 223
            D  A L+   E  +D ++ D +DP+  GP  +L+ K F++++ +  L   G+  TQA  
Sbjct: 225 GDGVAFLKNAAEGSYDAVIVDSSDPI--GPAKELFEKPFFQSVAR-ALRPGGVVCTQA-- 284

Query: 224 AGIYTHKEVFSSIYNTIRQVFKYVVAFT-AHVPSY-ADTWGWVMASDHPILIDAK 276
             ++ H ++   I +  R++FK  V +    VP+Y +   G+++ S     +D K
Sbjct: 285 ESLWLHMDIIEDIVSNCREIFKGSVNYAWTSVPTYPSGVIGFMLCSTEGPDVDFK 334

[Close]

BLAST of Spo19414.1 vs. TAIR (Arabidopsis)
Match: AT5G53120.6 (spermidine synthase 3)

HSP 1 Score: 117.9 bits (294), Expect = 1.400e-26
Identity = 84/293 (28.67%), Postives = 140/293 (47.78%), Query Frame = 1


		  
Query: 28  NNEFWYEEEIDEDLKWSFALKSVLQKATSEYQDIVLLDTKKFGKVLVLDGKMQSAEADEF 87
           NN  W  E        S  ++ VL K  S++Q++++ ++  +GKVLVLDG +Q  E DE 
Sbjct: 74  NNPMWPGEA------HSLKVEKVLFKDKSDFQEVLVFESATYGKVLVLDGIVQLTEKDEC 133

Query: 88  IYHECLIHPALLCHLNPKSMF---------------------------IMGGGEGSAARE 147
            Y E + H  L    +PK++                            ++GGG+G   RE
Sbjct: 134 AYQEMIAHLPLCSISSPKNVISHLKKFSLSGFLYLVACCNKSVNLKVLVVGGGDGGVLRE 193

Query: 148 ALKHKSIDKVVMCDIDQEVVDFCRKHLTINQEAFNNNKFELVINDAKAELEGRKE-KFDI 207
             +H S++ + +C+ID+ V+D  +K        F++ + +L I DA   L    E K+D 
Sbjct: 194 ISRHSSVEVIDICEIDKMVIDVSKKFFPELAVGFDDPRVQLHIGDAAEFLRKSPEGKYDA 253

Query: 208 IVGDLADPVEGGPCYQLYTKSFYETILKPRLTDNGIFVTQAGPAGIYTHKEVFSSIYNTI 267
           I+ D +DPV  GP   L  K F+ET+ +  L   G+    A    ++TH  +   + +  
Sbjct: 254 IIVDSSDPV--GPALALVEKPFFETLAR-ALKPGGVLCNMAESMWLHTH--LIEDMISIC 313

Query: 268 RQVFKYVVAFTAHVPSY-ADTWGWVMASDHPILIDAKDLDKKIQDRIDGELLY 292
           RQ FK V    + VP+Y +   G+V+ S     +D K+    I +++DG + +
Sbjct: 314 RQTFKSVHYAWSSVPTYPSGVIGFVLCSTEGPAVDFKNPINPI-EKLDGAMTH 354

The following BLAST results are available for this feature:

BLAST of Spo19414.1 vs. NCBI nr
BLAST of Spo19414.1 vs. UniProtKB/TrEMBL
BLAST of Spo19414.1 vs. ExPASy Swiss-Prot
BLAST of Spo19414.1 vs. TAIR (Arabidopsis)

BLAST of Spo19414.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|731317722\|ref\|XP_010669338.1\|	1.6e-170	88.9	PREDICTED: thermospermine synt...	[more]
gi\|731317720\|ref\|XP_010669337.1\|	4.0e-169	88.6	PREDICTED: thermospermine synt...	[more]
gi\|224088768\|ref\|XP_002308531.1\|	1.4e-150	76.4	hypothetical protein POPTR_000...	[more]
gi\|400268242\|gb\|AFP74573.1\|	3.2e-150	76.4	thermospermine synthase [Popul...	[more]
gi\|743812837\|ref\|XP_011019369.1\|	9.4e-150	75.8	PREDICTED: thermospermine synt...	[more]

BLAST of Spo19414.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0J8CW98_BETVU	1.1e-170	88.9	Uncharacterized protein OS=Bet...	[more]
A0A0J8D134_BETVU	2.8e-169	88.6	Uncharacterized protein OS=Bet...	[more]
B9HD20_POPTR	1.0e-150	76.4	Uncharacterized protein OS=Pop...	[more]
T1PPE6_9ROSI	2.2e-150	76.4	Thermospermine synthase OS=Pop...	[more]
A0A067KD25_JATCU	7.5e-146	75.3	Uncharacterized protein OS=Jat...	[more]

BLAST of Spo19414.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
ACL5_ARATH	1.2e-136	69.5	Thermospermine synthase ACAULI...	[more]
SPEE_SULTO	4.6e-56	42.5	Polyamine aminopropyltransfera...	[more]
SPEE_SULSO	9.6e-54	41.8	Polyamine aminopropyltransfera...	[more]
SPEE_AERPE	1.5e-51	37.5	Polyamine aminopropyltransfera...	[more]
SPEE_SULIM	2.5e-49	38.9	Polyamine aminopropyltransfera...	[more]

BLAST of Spo19414.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 4


Match Name	E-value	Identity	Description
AT5G19530.1	6.7e-138	69.5	S-adenosyl-L-methionine-depend...	[more]
AT1G70310.1	2.2e-32	31.0	spermidine synthase 2	[more]
AT1G23820.1	2.9e-32	32.3	spermidine synthase 1	[more]
AT5G53120.6	1.4e-26	28.6	spermidine synthase 3	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001045	Spermidine/spermine synthases	HAMAP	MF_00198	Spermidine_synth	coord: 31..313 score: 29
None	No IPR available	GENE3D	2.30.140.10		coord: 30..83 score: 7.1
None	No IPR available	PANTHER	PTHR11558	SPERMIDINE/SPERMINE SYNTHASE	coord: 32..336 score: 6.4E
None	No IPR available	PANTHER	PTHR11558:SF30	SUBFAMILY NOT NAMED	coord: 32..336 score: 6.4E
None	No IPR available	PFAM	PF01564	Spermine_synth	coord: 32..269 score: 1.6

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006596	polyamine biosynthetic process
biological_process	GO:0043481	anthocyanin accumulation in tissues in response to UV light
biological_process	GO:0048759	xylem vessel member cell differentiation
biological_process	GO:0048767	root hair elongation
biological_process	GO:0010218	response to far red light
biological_process	GO:0008361	regulation of cell size
biological_process	GO:0006560	proline metabolic process
biological_process	GO:0000271	polysaccharide biosynthetic process
biological_process	GO:0007389	pattern specification process
biological_process	GO:0009825	multidimensional cell growth
biological_process	GO:0010014	meristem initiation
biological_process	GO:0071555	cell wall organization
biological_process	GO:0009932	cell tip growth
biological_process	GO:0019482	beta-alanine metabolic process
biological_process	GO:0009926	auxin polar transport
biological_process	GO:0006525	arginine metabolic process
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0016768	spermine synthase activity
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0004766	spermidine synthase activity
molecular_function	GO:0010487	thermospermine synthase activity