Spo19561.1 (mRNA) | SpinachBase

Overview


Name	Spo19561.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	DNA-directed RNA polymerase subunit beta'' (2.7.7.6) (PEP) (Plastid-encoded RNA polymerase subunit beta'')
Location	Super_scaffold_229 : 401971 .. 406562 (+)
Sequence length	531

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGAAAGATCTTAAAGGTAACTTTTTTGTAGTTCCTTTCTTAATTTCCCCCAATAATTCAATTTCATTTCTTGCTATTTATCAAAATTTAGAGTAATTAAATCTTTCCAGTAATGGTTTATGTAATTGTGGCATATAAATCGAGGGTGAGGTTGGATTTGATTGTTGGAATTTTAGGGTTTGATAAGAGTGATTTGTCCGTAAATAAAGTAGGTGTGATCGAATATGTATACTCTTTCACGATAGGAAGAATTCGATTATAAAGAGTGCATTTTTTGTTCATTTTTAAATTTTGGAGTTTTAAGGGTTCTATCTTCATTTTTTTTATATAAAATATAGGGTAGTATTTGCATATTTTAGTGGTTTTTGTTCATTGTTTAGGGTAACAATGAACATTGAAAATGATATTTATGATACTAGATCTACTATGTTTCCTATGATATAGTTAGTTCTGCATATAATGGTATGTATTGGAAAGGGCTTCCAGAATTGAATGTGGCTGCCTACTGATTTGAAGATGTTGCTACTCTCTCTCCGTCCAAATAATGCTTAAAAACGTTTTGGTTAGTGTTTGATTTTTTGACAAAGTAATTTTACCAATACCTACTTCAATTTTCAGGATTTTGATTGTAATTTTATGGTTTGGTTGTGTTGCGTTCAATCTTTAGGGTACTACGGACTTTTTAATTATTTTTTGTGATTTGGATAGTTATTGTGCTTTTTCGTGTTGTAGAGTTTGATCGGAAATATATTGTGTATTGATGCCGGAAACTGTTATGGAATTTAACACTCTAGATTTTTTTAGACGTAGTTCTAGGAATCAATCTGGAAAAGAGAAGAATAAAGCTGAAAGAAAAACGTCATTGGAGAAGTAAAGTTTAATGTTAGTTTAATTTGGAGCTTTGCCTTTGTTTTTAAGTTGATTTTCTATTTTTTCACATCAGTTTTATTCATAGCTAATGCATTACCTAGAATTGGAGTTACATGCTTGATTTTCTAGACGATTGAGGTAATGAACTGCATTTACAAATGATGGGAAATTGCTATGGAAGGTTGTTCTTCCTGGTTGGTTGTTATACTTGTAATAAGATAAACTATTTAGGGCTAGTCCTTTCTATATATAATTAGATACAAGTTTATGACTAGAAAATTGTCTTAATTCTTTAACATTTCTTACAAACTCCTAGAAATCAATCCAAATTCCTAGGCTGTGTTATGGCTTTTTTACACCAGAAGATAAGGGATTCATATACTAACTCTCATTTTAACGCAAATGGCCGTGAAGCCTTTTCAGATTGAGCACTTGTAATGGATCAAGAAGTCTTTAATCTATCCGGTCGATAACTTTGTGTCTAGGAAAGGTAGGGTTCGATATTCTCATGGTTTCCCCTCTTTTCTGCATCCTGCCTTCTATATATTTTCCCCGTCCTTGGTTATTATCACATTATATACATGAGGGATCATTGTGAATTATCGTAGCAACTGGTGGTATATTTCTTATAAAATCCTGTGATATGGATCTTTGTTTAAACTTTTAGTGATTGGTTGATATGTATGTTCCATAAGATGGCACATTTAACATTAAAACATGTTGTGCTCATGCAGCCTGTAGATTATAATTATTTGATAGATGAATTGAAATTGATCAATTTTTGTGAGATATACATTTTATCTAGATAAATAAATGACTAGATATGGACAAGATTGAAATGGAGAAGTAAAGACGACACATATTAATCTTGGTTGAATTCAGCAGTTCCCTATGCTTATTTTTATAATTTTGATGTCATATTTTCGGTACTAATTTGCAATTATTATTATTTTTAAATATTTACAATTATATTTTAAGCTCTTAGTGTATGCTGGTTGAGGAAGGATGACTGAAAAGGTTTGCATTTGTCTTAGTTTGCCTATATCGATTTTTTTTTTTTTTTGGTATAATGGTTAACAAAATGCCCTGAATAACAATGCACTTTTACAAACTCTGTAATTGCTAATGTGGAGTCTTATGATCTAGTTTGCTTTTAATAATTCTGGAACTAAGAAATGGTGGTTGCCCATTTGGATCAAACTTGAGTATGCTTTAGGTTGGCTGGGAATGAATGTTTCAAGGTATTTTTCATTAAACCTTTGATCATTGTATTAACAAAACAATGTTGCTACAAATCCTGTTCATTTAGATCCAGTTGAATATGAATGTTAAGAGATGGCCCTTTGAGTCACGAATGTTGATTATCTTCTACTTGGAAGCTCTTCTTGTACTCGTTTGATGTTTTATTGTACCCAAGTTTTGGACAAAAGGTGGTGTAAATATTTACACCTTATTTTTTATGCACTCAACTAGGACTTTACTAATGTAGTCAATATTTCATAATTTTAAGGAGCAAAGTTCTATGTATTGGAGGTGGAATGACAGATACATGGCAGTGAAGCTACTGTCACAGAAAGTGAGACTAAGGAGATGGACGGTCCAAGTTCAGGCGCAACTATGTAAGTGTGTCTTGTGATTGATTTGTAATTCTAAACTCAATATCGGTTTATTTGTATATTAAATATACCCTCTATCTTTAAGTTTCCATTTTGTTCATTTTTATAGGTTCTGTTTGATTTCATGAATCAATTGGAAGAAAGGAAGGCAGAAATGGAGTAACATAGACGTATGAGGATTGAATCTCATGGCATACAGATAACTAGTCTCTGGAATGTAATGTGTAGATATTTCCATAGGAGTTTTTACTTTGATAGCTAACTTGGTAGTGTTTCACTTTGTAAGCTTATTAAAGGGTAATGATAAATTACATGTATATACATTTTCAATATCAATAAAGTATCATTTCTTACTATCACGAATTATTTCTTATTAGGTTCACATAATTTTAGAAAAATATAAATTTGATTAGGTAGGATTTGTTGCTAATGGAGCCGTTGCAATAGGTACCAACAATATTTTTTTTACGATAAATTACCGTTGCATTTAGGGAAAAAAGCGTTGCATTATCTCATTAAGCCATCGTACATACCTGCCGCATTAGCTAAAACTTCCGTCACATTAGCTAAAATACCCATCGCATTAGCTAAAATATCCGTCGCATTAACTAAAATAACCGTCGCATTATCTAGAACTTTCATCGCATTAGCTAAATAACTAATGCGACACCCCAGCTAAGGACTGTCGCATTAGATGTCCACCTAATGCAACGGTAGTAATTTACCGTCGCATCAGCTCATTGATCGTTGCATTAGGCCTAATGCAACGCCACCGGATGCAAGGGTCATTTGTTGTCGCATTAGGTCTAATGCAACGGATTTCCATGCCTACTGCAACGATTTTAGGCGTCGCATCTGATTAGAAATGTAGTAGTGTTTCTCCACCATGGAAGCCAAATCCTCGTCATCGACATTTTCTCTGTGTTGGACTGAATTTGTGCATTGTGTAGATTTAATCGAAACAAATTAAATCGCAGTTTAATTATAAATTGTTGGAAATTTGTGCAATTATTAGGATAAGATTATGATTAAAATTTAGAAGTTGAAGAATAAATGAAGAGGACAAAGGAAGAAACAGGGGTTAGAGTTGGAAGAGGAGAGAGAAAGGAGTTGATGCTGGGATTTGGTTTACCCAGGCAACGATTTTGTTTCTTTTTAATTGATGATTAATTTCATGCCGTGAGATCAATATGTTATGTACGGTTGAGATCTCTTCTCATCCTTCTCATTATAAGGACCTTCTCATTGGAGGGGGACCTATATATATATATAAGGACGTCCTTTTAGGAATTCATTTGGTCCTTTAGGAACAGCCCTTCAAATTGCTAATTTTTTTTCATTTTACTATTTAATAACTCATAATCAGATCTTGGTAACTAATTATTTGCAACTTGACAATTTAAAACAAACCTTTCAACCATTTAAATTTCAATATTATTTAATCGATGAAAATGGAAGAATTTATAATCCCGATCCATGCAGTAACATCATTTTTAATCCATTCAAGTTGAATTGGTATTTTCTTCATTATCATTTTTGTGAAGAGACATCCACCAAAATTGATCTTGGACAATTTGTTTGTGAAAATGTATGTATAACCAAAAAAGGAACGCACCTAAAATCGGGTCAAGTTCTAATTGTTCAATTTGACTCTGTAGTAATAAGATTGGCTAAGCCTTATTTGGCTACTCCAGGAGCAACACTTCATGGTCATTATGGAGAAATCATTTATGAAGGGGATACATTAGTTACATTTATATATGAAAAATCGAGGTCTGGTGACATAACGCAAGGTCTTCCAAAAGTGGAACAAGTCTTAGAAGTTCGTTCGATTGATTCAATATCCATCAATCTAGAAAAGAGGATTGATAGTTGGAACGAACGTATAACAAGAATTCTTGGAAGTCCTTGGGGATTCTTGATTGGGGCTGAGCTAACTATAGCGCAAAGTCGTATTTCTTTGGTTAACAAGATCCAAAAGGTTTATCGATCACAAGGGGTGCAGATCCATAATAGACATATAGAAATTATTGTACGTCAAATAACATCAAAAGTCTTGGTTTCAGAAGATGGAATGTCTAATGTTTTTTTCCCGGAGAACTAA

mRNA sequence

ATGAGAAAGATCTTAAAGATACATGGCAGTGAAGCTACTGTCACAGAAAGTGAGACTAAGGAGATGGACGGTCCAAGTTCAGGCGCAACTATATTGGCTAAGCCTTATTTGGCTACTCCAGGAGCAACACTTCATGGTCATTATGGAGAAATCATTTATGAAGGGGATACATTAGTTACATTTATATATGAAAAATCGAGGTCTGGTGACATAACGCAAGGTCTTCCAAAAGTGGAACAAGTCTTAGAAGTTCGTTCGATTGATTCAATATCCATCAATCTAGAAAAGAGGATTGATAGTTGGAACGAACGTATAACAAGAATTCTTGGAAGTCCTTGGGGATTCTTGATTGGGGCTGAGCTAACTATAGCGCAAAGTCGTATTTCTTTGGTTAACAAGATCCAAAAGGTTTATCGATCACAAGGGGTGCAGATCCATAATAGACATATAGAAATTATTGTACGTCAAATAACATCAAAAGTCTTGGTTTCAGAAGATGGAATGTCTAATGTTTTTTTCCCGGAGAACTAA

Coding sequence (CDS)

ATGAGAAAGATCTTAAAGATACATGGCAGTGAAGCTACTGTCACAGAAAGTGAGACTAAGGAGATGGACGGTCCAAGTTCAGGCGCAACTATATTGGCTAAGCCTTATTTGGCTACTCCAGGAGCAACACTTCATGGTCATTATGGAGAAATCATTTATGAAGGGGATACATTAGTTACATTTATATATGAAAAATCGAGGTCTGGTGACATAACGCAAGGTCTTCCAAAAGTGGAACAAGTCTTAGAAGTTCGTTCGATTGATTCAATATCCATCAATCTAGAAAAGAGGATTGATAGTTGGAACGAACGTATAACAAGAATTCTTGGAAGTCCTTGGGGATTCTTGATTGGGGCTGAGCTAACTATAGCGCAAAGTCGTATTTCTTTGGTTAACAAGATCCAAAAGGTTTATCGATCACAAGGGGTGCAGATCCATAATAGACATATAGAAATTATTGTACGTCAAATAACATCAAAAGTCTTGGTTTCAGAAGATGGAATGTCTAATGTTTTTTTCCCGGAGAACTAA

Protein sequence

MRKILKIHGSEATVTESETKEMDGPSSGATILAKPYLATPGATLHGHYGEIIYEGDTLVTFIYEKSRSGDITQGLPKVEQVLEVRSIDSISINLEKRIDSWNERITRILGSPWGFLIGAELTIAQSRISLVNKIQKVYRSQGVQIHNRHIEIIVRQITSKVLVSEDGMSNVFFPEN

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo19561	Spo19561	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo19561.1	Spo19561.1-protein	polypeptide

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo19561.1.exon.1	Spo19561.1.exon.1	exon
Spo19561.1.exon.2	Spo19561.1.exon.2	exon
Spo19561.1.exon.3	Spo19561.1.exon.3	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo19561.1.CDS.1	Spo19561.1.CDS.1	CDS
Spo19561.1.CDS.2	Spo19561.1.CDS.2	CDS
Spo19561.1.CDS.3	Spo19561.1.CDS.3	CDS

Homology

[Close]

BLAST of Spo19561.1 vs. NCBI nr
Match: gi|126022792|ref|NP_054922.2| (RNA polymerase beta'' subunit [Spinacia oleracea])

HSP 1 Score: 280.8 bits (717), Expect = 1.600e-72
Identity = 141/142 (99.30%), Postives = 141/142 (99.30%), Query Frame = 1


		  
Query: 33   AKPYLATPGATLHGHYGEIIYEGDTLVTFIYEKSRSGDITQGLPKVEQVLEVRSIDSISI 92
            AKPYLATPGATLHGHYGEIIYEGDTLVTFIYEKSRSGDITQGLPKVEQVLEVRSIDSISI
Sbjct: 1088 AKPYLATPGATLHGHYGEIIYEGDTLVTFIYEKSRSGDITQGLPKVEQVLEVRSIDSISI 1147

Query: 93   NLEKRIDSWNERITRILGSPWGFLIGAELTIAQSRISLVNKIQKVYRSQGVQIHNRHIEI 152
            NLEKRIDSWNERITRILGSPWGFLIGAELTIAQSRISLVNKIQKVYRSQGVQIHNRHIEI
Sbjct: 1148 NLEKRIDSWNERITRILGSPWGFLIGAELTIAQSRISLVNKIQKVYRSQGVQIHNRHIEI 1207

Query: 153  IVRQITSKVLVSEDGMSNVFFP 175
            IVRQITSKVLVSEDGMSNVF P
Sbjct: 1208 IVRQITSKVLVSEDGMSNVFLP 1229

[Close]

BLAST of Spo19561.1 vs. NCBI nr
Match: gi|133448|sp|P11704.1|RPOC2_SPIOL (RecName: Full=DNA-directed RNA polymerase subunit beta''; AltName: Full=PEP; AltName: Full=Plastid-encoded RNA polymerase subunit beta''; Short=RNA polymerase subunit beta'' (chloroplast) [Spinacia oleracea])

HSP 1 Score: 280.8 bits (717), Expect = 1.600e-72
Identity = 141/142 (99.30%), Postives = 141/142 (99.30%), Query Frame = 1


		  
Query: 33   AKPYLATPGATLHGHYGEIIYEGDTLVTFIYEKSRSGDITQGLPKVEQVLEVRSIDSISI 92
            AKPYLATPGATLHGHYGEIIYEGDTLVTFIYEKSRSGDITQGLPKVEQVLEVRSIDSISI
Sbjct: 1092 AKPYLATPGATLHGHYGEIIYEGDTLVTFIYEKSRSGDITQGLPKVEQVLEVRSIDSISI 1151

Query: 93   NLEKRIDSWNERITRILGSPWGFLIGAELTIAQSRISLVNKIQKVYRSQGVQIHNRHIEI 152
            NLEKRIDSWNERITRILGSPWGFLIGAELTIAQSRISLVNKIQKVYRSQGVQIHNRHIEI
Sbjct: 1152 NLEKRIDSWNERITRILGSPWGFLIGAELTIAQSRISLVNKIQKVYRSQGVQIHNRHIEI 1211

Query: 153  IVRQITSKVLVSEDGMSNVFFP 175
            IVRQITSKVLVSEDGMSNVF P
Sbjct: 1212 IVRQITSKVLVSEDGMSNVFLP 1233

[Close]

BLAST of Spo19561.1 vs. NCBI nr
Match: gi|90403635|gb|ABD93711.1| (RNA polymerase beta chain, partial (chloroplast) [Spinacia oleracea])

HSP 1 Score: 280.8 bits (717), Expect = 1.600e-72
Identity = 141/142 (99.30%), Postives = 141/142 (99.30%), Query Frame = 1


		  
Query: 33  AKPYLATPGATLHGHYGEIIYEGDTLVTFIYEKSRSGDITQGLPKVEQVLEVRSIDSISI 92
           AKPYLATPGATLHGHYGEIIYEGDTLVTFIYEKSRSGDITQGLPKVEQVLEVRSIDSISI
Sbjct: 630 AKPYLATPGATLHGHYGEIIYEGDTLVTFIYEKSRSGDITQGLPKVEQVLEVRSIDSISI 689

Query: 93  NLEKRIDSWNERITRILGSPWGFLIGAELTIAQSRISLVNKIQKVYRSQGVQIHNRHIEI 152
           NLEKRIDSWNERITRILGSPWGFLIGAELTIAQSRISLVNKIQKVYRSQGVQIHNRHIEI
Sbjct: 690 NLEKRIDSWNERITRILGSPWGFLIGAELTIAQSRISLVNKIQKVYRSQGVQIHNRHIEI 749

Query: 153 IVRQITSKVLVSEDGMSNVFFP 175
           IVRQITSKVLVSEDGMSNVF P
Sbjct: 750 IVRQITSKVLVSEDGMSNVFLP 771

[Close]

BLAST of Spo19561.1 vs. NCBI nr
Match: gi|937546352|ref|YP_009169346.1| (RNA polymerase beta'' subunit (chloroplast) [Clematis terniflora])

HSP 1 Score: 272.7 bits (696), Expect = 4.500e-70
Identity = 135/142 (95.07%), Postives = 140/142 (98.59%), Query Frame = 1


		  
Query: 33   AKPYLATPGATLHGHYGEIIYEGDTLVTFIYEKSRSGDITQGLPKVEQVLEVRSIDSISI 92
            AKPYLATPGAT+HGHYGEI+YEGDTLVTFIYEKSRSGDITQGLPKVEQVLEVRSIDSIS+
Sbjct: 1099 AKPYLATPGATVHGHYGEILYEGDTLVTFIYEKSRSGDITQGLPKVEQVLEVRSIDSISM 1158

Query: 93   NLEKRIDSWNERITRILGSPWGFLIGAELTIAQSRISLVNKIQKVYRSQGVQIHNRHIEI 152
            NLEKR++SWNERITRILG PWGFLIGAELTIAQSRISLVNKIQKVYRSQGVQIHNRHIEI
Sbjct: 1159 NLEKRVESWNERITRILGIPWGFLIGAELTIAQSRISLVNKIQKVYRSQGVQIHNRHIEI 1218

Query: 153  IVRQITSKVLVSEDGMSNVFFP 175
            IVRQITSKVLVSEDGMSNVF P
Sbjct: 1219 IVRQITSKVLVSEDGMSNVFSP 1240

[Close]

BLAST of Spo19561.1 vs. NCBI nr
Match: gi|844170325|gb|AKM98248.1| (RNA polymerase beta subunit-2 (chloroplast) [Pulsatilla pratensis])

HSP 1 Score: 272.7 bits (696), Expect = 4.500e-70
Identity = 135/142 (95.07%), Postives = 140/142 (98.59%), Query Frame = 1


		  
Query: 33   AKPYLATPGATLHGHYGEIIYEGDTLVTFIYEKSRSGDITQGLPKVEQVLEVRSIDSISI 92
            AKPYLATPGAT+HGHYGEI+YEGDTLVTFIYEKSRSGDITQGLPKVEQVLEVRSIDSIS+
Sbjct: 1101 AKPYLATPGATVHGHYGEILYEGDTLVTFIYEKSRSGDITQGLPKVEQVLEVRSIDSISM 1160

Query: 93   NLEKRIDSWNERITRILGSPWGFLIGAELTIAQSRISLVNKIQKVYRSQGVQIHNRHIEI 152
            NLEKR++SWNERITRILG PWGFLIGAELTIAQSRISLVNKIQKVYRSQGVQIHNRHIEI
Sbjct: 1161 NLEKRVESWNERITRILGIPWGFLIGAELTIAQSRISLVNKIQKVYRSQGVQIHNRHIEI 1220

Query: 153  IVRQITSKVLVSEDGMSNVFFP 175
            IVRQITSKVLVSEDGMSNVF P
Sbjct: 1221 IVRQITSKVLVSEDGMSNVFSP 1242

[Close]

BLAST of Spo19561.1 vs. UniProtKB/TrEMBL
Match: A1XK05_SPIOL (RNA polymerase beta chain (Fragment) OS=Spinacia oleracea GN=rpoC2 PE=4 SV=1)

HSP 1 Score: 280.8 bits (717), Expect = 1.100e-72
Identity = 141/142 (99.30%), Postives = 141/142 (99.30%), Query Frame = 1


		  
Query: 33  AKPYLATPGATLHGHYGEIIYEGDTLVTFIYEKSRSGDITQGLPKVEQVLEVRSIDSISI 92
           AKPYLATPGATLHGHYGEIIYEGDTLVTFIYEKSRSGDITQGLPKVEQVLEVRSIDSISI
Sbjct: 630 AKPYLATPGATLHGHYGEIIYEGDTLVTFIYEKSRSGDITQGLPKVEQVLEVRSIDSISI 689

Query: 93  NLEKRIDSWNERITRILGSPWGFLIGAELTIAQSRISLVNKIQKVYRSQGVQIHNRHIEI 152
           NLEKRIDSWNERITRILGSPWGFLIGAELTIAQSRISLVNKIQKVYRSQGVQIHNRHIEI
Sbjct: 690 NLEKRIDSWNERITRILGSPWGFLIGAELTIAQSRISLVNKIQKVYRSQGVQIHNRHIEI 749

Query: 153 IVRQITSKVLVSEDGMSNVFFP 175
           IVRQITSKVLVSEDGMSNVF P
Sbjct: 750 IVRQITSKVLVSEDGMSNVFLP 771

[Close]

BLAST of Spo19561.1 vs. UniProtKB/TrEMBL
Match: A0A0M3LBE2_CLETE (DNA-directed RNA polymerase subunit beta'' OS=Clematis terniflora GN=rpoC2 PE=3 SV=1)

HSP 1 Score: 272.7 bits (696), Expect = 3.100e-70
Identity = 135/142 (95.07%), Postives = 140/142 (98.59%), Query Frame = 1


		  
Query: 33   AKPYLATPGATLHGHYGEIIYEGDTLVTFIYEKSRSGDITQGLPKVEQVLEVRSIDSISI 92
            AKPYLATPGAT+HGHYGEI+YEGDTLVTFIYEKSRSGDITQGLPKVEQVLEVRSIDSIS+
Sbjct: 1099 AKPYLATPGATVHGHYGEILYEGDTLVTFIYEKSRSGDITQGLPKVEQVLEVRSIDSISM 1158

Query: 93   NLEKRIDSWNERITRILGSPWGFLIGAELTIAQSRISLVNKIQKVYRSQGVQIHNRHIEI 152
            NLEKR++SWNERITRILG PWGFLIGAELTIAQSRISLVNKIQKVYRSQGVQIHNRHIEI
Sbjct: 1159 NLEKRVESWNERITRILGIPWGFLIGAELTIAQSRISLVNKIQKVYRSQGVQIHNRHIEI 1218

Query: 153  IVRQITSKVLVSEDGMSNVFFP 175
            IVRQITSKVLVSEDGMSNVF P
Sbjct: 1219 IVRQITSKVLVSEDGMSNVFSP 1240

[Close]

BLAST of Spo19561.1 vs. UniProtKB/TrEMBL
Match: A0A0H3Y3A9_9MAGN (DNA-directed RNA polymerase subunit beta'' OS=Anemone patens GN=rpoC2 PE=3 SV=1)

HSP 1 Score: 272.7 bits (696), Expect = 3.100e-70
Identity = 135/142 (95.07%), Postives = 140/142 (98.59%), Query Frame = 1


		  
Query: 33   AKPYLATPGATLHGHYGEIIYEGDTLVTFIYEKSRSGDITQGLPKVEQVLEVRSIDSISI 92
            AKPYLATPGAT+HGHYGEI+YEGDTLVTFIYEKSRSGDITQGLPKVEQVLEVRSIDSIS+
Sbjct: 1099 AKPYLATPGATVHGHYGEILYEGDTLVTFIYEKSRSGDITQGLPKVEQVLEVRSIDSISM 1158

Query: 93   NLEKRIDSWNERITRILGSPWGFLIGAELTIAQSRISLVNKIQKVYRSQGVQIHNRHIEI 152
            NLEKR++SWNERITRILG PWGFLIGAELTIAQSRISLVNKIQKVYRSQGVQIHNRHIEI
Sbjct: 1159 NLEKRVESWNERITRILGIPWGFLIGAELTIAQSRISLVNKIQKVYRSQGVQIHNRHIEI 1218

Query: 153  IVRQITSKVLVSEDGMSNVFFP 175
            IVRQITSKVLVSEDGMSNVF P
Sbjct: 1219 IVRQITSKVLVSEDGMSNVFSP 1240

[Close]

BLAST of Spo19561.1 vs. UniProtKB/TrEMBL
Match: A0A0H3Y388_9MAGN (DNA-directed RNA polymerase subunit beta'' OS=Pulsatilla vernalis GN=rpoC2 PE=3 SV=1)

HSP 1 Score: 272.7 bits (696), Expect = 3.100e-70
Identity = 135/142 (95.07%), Postives = 140/142 (98.59%), Query Frame = 1


		  
Query: 33   AKPYLATPGATLHGHYGEIIYEGDTLVTFIYEKSRSGDITQGLPKVEQVLEVRSIDSISI 92
            AKPYLATPGAT+HGHYGEI+YEGDTLVTFIYEKSRSGDITQGLPKVEQVLEVRSIDSIS+
Sbjct: 1099 AKPYLATPGATVHGHYGEILYEGDTLVTFIYEKSRSGDITQGLPKVEQVLEVRSIDSISM 1158

Query: 93   NLEKRIDSWNERITRILGSPWGFLIGAELTIAQSRISLVNKIQKVYRSQGVQIHNRHIEI 152
            NLEKR++SWNERITRILG PWGFLIGAELTIAQSRISLVNKIQKVYRSQGVQIHNRHIEI
Sbjct: 1159 NLEKRVESWNERITRILGIPWGFLIGAELTIAQSRISLVNKIQKVYRSQGVQIHNRHIEI 1218

Query: 153  IVRQITSKVLVSEDGMSNVFFP 175
            IVRQITSKVLVSEDGMSNVF P
Sbjct: 1219 IVRQITSKVLVSEDGMSNVFSP 1240

[Close]

BLAST of Spo19561.1 vs. UniProtKB/TrEMBL
Match: H6T9I2_9CARY (DNA-directed RNA polymerase beta' chain (Fragment) OS=Didierea madagascariensis GN=rpoC2 PE=4 SV=1)

HSP 1 Score: 272.7 bits (696), Expect = 3.100e-70
Identity = 136/142 (95.77%), Postives = 138/142 (97.18%), Query Frame = 1


		  
Query: 33  AKPYLATPGATLHGHYGEIIYEGDTLVTFIYEKSRSGDITQGLPKVEQVLEVRSIDSISI 92
           AKPYLATPGAT+HGHYGEIIYEGDTLVTFIYEKSRSGDITQGLPKVEQVLEVRSIDSIS+
Sbjct: 630 AKPYLATPGATVHGHYGEIIYEGDTLVTFIYEKSRSGDITQGLPKVEQVLEVRSIDSISM 689

Query: 93  NLEKRIDSWNERITRILGSPWGFLIGAELTIAQSRISLVNKIQKVYRSQGVQIHNRHIEI 152
           NLEKRID WNE ITRILG PWGFLIGAELTIAQSRISLVNKIQKVYRSQGVQIHNRHIEI
Sbjct: 690 NLEKRIDGWNEHITRILGIPWGFLIGAELTIAQSRISLVNKIQKVYRSQGVQIHNRHIEI 749

Query: 153 IVRQITSKVLVSEDGMSNVFFP 175
           IVRQITSKVLVSEDGMSNVF P
Sbjct: 750 IVRQITSKVLVSEDGMSNVFLP 771

[Close]

BLAST of Spo19561.1 vs. ExPASy Swiss-Prot
Match: RPOC2_SPIOL (DNA-directed RNA polymerase subunit beta'' OS=Spinacia oleracea GN=rpoC2 PE=3 SV=1)

HSP 1 Score: 280.8 bits (717), Expect = 1.000e-74
Identity = 141/142 (99.30%), Postives = 141/142 (99.30%), Query Frame = 1


		  
Query: 33   AKPYLATPGATLHGHYGEIIYEGDTLVTFIYEKSRSGDITQGLPKVEQVLEVRSIDSISI 92
            AKPYLATPGATLHGHYGEIIYEGDTLVTFIYEKSRSGDITQGLPKVEQVLEVRSIDSISI
Sbjct: 1092 AKPYLATPGATLHGHYGEIIYEGDTLVTFIYEKSRSGDITQGLPKVEQVLEVRSIDSISI 1151

Query: 93   NLEKRIDSWNERITRILGSPWGFLIGAELTIAQSRISLVNKIQKVYRSQGVQIHNRHIEI 152
            NLEKRIDSWNERITRILGSPWGFLIGAELTIAQSRISLVNKIQKVYRSQGVQIHNRHIEI
Sbjct: 1152 NLEKRIDSWNERITRILGSPWGFLIGAELTIAQSRISLVNKIQKVYRSQGVQIHNRHIEI 1211

Query: 153  IVRQITSKVLVSEDGMSNVFFP 175
            IVRQITSKVLVSEDGMSNVF P
Sbjct: 1212 IVRQITSKVLVSEDGMSNVFLP 1233

[Close]

BLAST of Spo19561.1 vs. ExPASy Swiss-Prot
Match: RPOC2_CHLSC (DNA-directed RNA polymerase subunit beta'' OS=Chloranthus spicatus GN=rpoC2 PE=3 SV=1)

HSP 1 Score: 269.6 bits (688), Expect = 2.400e-71
Identity = 133/142 (93.66%), Postives = 139/142 (97.89%), Query Frame = 1


		  
Query: 33   AKPYLATPGATLHGHYGEIIYEGDTLVTFIYEKSRSGDITQGLPKVEQVLEVRSIDSISI 92
            AKP+LATPGAT+HGHYGEI+YEGDTLVTFIYEKSRSGDITQGLPKVEQVLEVRSIDSISI
Sbjct: 1103 AKPHLATPGATVHGHYGEILYEGDTLVTFIYEKSRSGDITQGLPKVEQVLEVRSIDSISI 1162

Query: 93   NLEKRIDSWNERITRILGSPWGFLIGAELTIAQSRISLVNKIQKVYRSQGVQIHNRHIEI 152
            NLEKR++ WNERITRI+G PWGFLIGAELTIAQSRISLVNKIQKVYRSQGVQIHNRHIEI
Sbjct: 1163 NLEKRVEGWNERITRIIGIPWGFLIGAELTIAQSRISLVNKIQKVYRSQGVQIHNRHIEI 1222

Query: 153  IVRQITSKVLVSEDGMSNVFFP 175
            IVRQITSKVLVSEDGMSNVF P
Sbjct: 1223 IVRQITSKVLVSEDGMSNVFSP 1244

[Close]

BLAST of Spo19561.1 vs. ExPASy Swiss-Prot
Match: RPOC2_NANDO (DNA-directed RNA polymerase subunit beta'' OS=Nandina domestica GN=rpoC2 PE=3 SV=1)

HSP 1 Score: 269.2 bits (687), Expect = 3.100e-71
Identity = 132/142 (92.96%), Postives = 139/142 (97.89%), Query Frame = 1


		  
Query: 33   AKPYLATPGATLHGHYGEIIYEGDTLVTFIYEKSRSGDITQGLPKVEQVLEVRSIDSISI 92
            AKPYLATPGAT+HGHYGEI+Y+GDTLVTFIYEKSRSGDITQGLPKVEQVLEVRSIDSIS+
Sbjct: 1093 AKPYLATPGATVHGHYGEILYKGDTLVTFIYEKSRSGDITQGLPKVEQVLEVRSIDSISM 1152

Query: 93   NLEKRIDSWNERITRILGSPWGFLIGAELTIAQSRISLVNKIQKVYRSQGVQIHNRHIEI 152
            NLEKR++ WNERITRILG PWGFLIGAELTIAQSR+SLVNKIQKVYRSQGVQIHNRHIEI
Sbjct: 1153 NLEKRVEGWNERITRILGIPWGFLIGAELTIAQSRVSLVNKIQKVYRSQGVQIHNRHIEI 1212

Query: 153  IVRQITSKVLVSEDGMSNVFFP 175
            IVRQITSKVLVSEDGMSNVF P
Sbjct: 1213 IVRQITSKVLVSEDGMSNVFSP 1234

[Close]

BLAST of Spo19561.1 vs. ExPASy Swiss-Prot
Match: RPOC2_POPAL (DNA-directed RNA polymerase subunit beta'' OS=Populus alba GN=rpoC2 PE=3 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 5.300e-71
Identity = 133/143 (93.01%), Postives = 139/143 (97.20%), Query Frame = 1


		  
Query: 32   LAKPYLATPGATLHGHYGEIIYEGDTLVTFIYEKSRSGDITQGLPKVEQVLEVRSIDSIS 91
            LAKPYLATPGAT+HGHYGEI+YEGDTLVTFIYEKSRSGDITQGLPKVEQVLEVRSIDSIS
Sbjct: 1105 LAKPYLATPGATVHGHYGEILYEGDTLVTFIYEKSRSGDITQGLPKVEQVLEVRSIDSIS 1164

Query: 92   INLEKRIDSWNERITRILGSPWGFLIGAELTIAQSRISLVNKIQKVYRSQGVQIHNRHIE 151
            INLEKR+++WNE ITRI+G PWGFLIGAELTI QSRISLVNKIQKVYRSQGVQIHNRHIE
Sbjct: 1165 INLEKRVENWNECITRIVGIPWGFLIGAELTIVQSRISLVNKIQKVYRSQGVQIHNRHIE 1224

Query: 152  IIVRQITSKVLVSEDGMSNVFFP 175
            IIVRQITSKVLVSEDGMSNVF P
Sbjct: 1225 IIVRQITSKVLVSEDGMSNVFSP 1247

[Close]

BLAST of Spo19561.1 vs. ExPASy Swiss-Prot
Match: RPOC2_POPTR (DNA-directed RNA polymerase subunit beta'' OS=Populus trichocarpa GN=rpoC2 PE=3 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 5.300e-71
Identity = 133/143 (93.01%), Postives = 139/143 (97.20%), Query Frame = 1


		  
Query: 32   LAKPYLATPGATLHGHYGEIIYEGDTLVTFIYEKSRSGDITQGLPKVEQVLEVRSIDSIS 91
            LAKPYLATPGAT+HGHYGEI+YEGDTLVTFIYEKSRSGDITQGLPKVEQVLEVRSIDSIS
Sbjct: 1105 LAKPYLATPGATVHGHYGEILYEGDTLVTFIYEKSRSGDITQGLPKVEQVLEVRSIDSIS 1164

Query: 92   INLEKRIDSWNERITRILGSPWGFLIGAELTIAQSRISLVNKIQKVYRSQGVQIHNRHIE 151
            INLEKR+++WNE ITRI+G PWGFLIGAELTI QSRISLVNKIQKVYRSQGVQIHNRHIE
Sbjct: 1165 INLEKRVENWNECITRIVGIPWGFLIGAELTIVQSRISLVNKIQKVYRSQGVQIHNRHIE 1224

Query: 152  IIVRQITSKVLVSEDGMSNVFFP 175
            IIVRQITSKVLVSEDGMSNVF P
Sbjct: 1225 IIVRQITSKVLVSEDGMSNVFSP 1247

[Close]

BLAST of Spo19561.1 vs. TAIR (Arabidopsis)
Match: ATCG00170.1 (DNA-directed RNA polymerase family protein)

HSP 1 Score: 259.2 bits (661), Expect = 1.800e-69
Identity = 129/142 (90.85%), Postives = 134/142 (94.37%), Query Frame = 1


		  
Query: 33   AKPYLATPGATLHGHYGEIIYEGDTLVTFIYEKSRSGDITQGLPKVEQVLEVRSIDSISI 92
            AKPYLATPGA +HGHY EI+YEGDTLVTFIYEKSRSGDITQGLPKVEQVLEVRSIDSIS+
Sbjct: 1099 AKPYLATPGAKVHGHYSEILYEGDTLVTFIYEKSRSGDITQGLPKVEQVLEVRSIDSISL 1158

Query: 93   NLEKRIDSWNERITRILGSPWGFLIGAELTIAQSRISLVNKIQKVYRSQGVQIHNRHIEI 152
            NLEKRI  WN+ ITRILG PWGFLIGAELTI QSRISLVNKIQKVYRSQGVQIHNRHIEI
Sbjct: 1159 NLEKRIKGWNKCITRILGIPWGFLIGAELTIVQSRISLVNKIQKVYRSQGVQIHNRHIEI 1218

Query: 153  IVRQITSKVLVSEDGMSNVFFP 175
            IVRQITSKVLVSE+GMSNVF P
Sbjct: 1219 IVRQITSKVLVSEEGMSNVFLP 1240

The following BLAST results are available for this feature:

BLAST of Spo19561.1 vs. NCBI nr
BLAST of Spo19561.1 vs. UniProtKB/TrEMBL
BLAST of Spo19561.1 vs. ExPASy Swiss-Prot
BLAST of Spo19561.1 vs. TAIR (Arabidopsis)

BLAST of Spo19561.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|126022792\|ref\|NP_054922.2\|	1.6e-72	99.3	RNA polymerase beta'' subunit ...	[more]
gi\|133448\|sp\|P11704.1\|RPOC2_SPIOL	1.6e-72	99.3	RecName: Full=DNA-directed RNA...	[more]
gi\|90403635\|gb\|ABD93711.1\|	1.6e-72	99.3	RNA polymerase beta chain, par...	[more]
gi\|937546352\|ref\|YP_009169346.1\|	4.5e-70	95.0	RNA polymerase beta'' subunit ...	[more]
gi\|844170325\|gb\|AKM98248.1\|	4.5e-70	95.0	RNA polymerase beta subunit-2 ...	[more]

BLAST of Spo19561.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A1XK05_SPIOL	1.1e-72	99.3	RNA polymerase beta chain (Fra...	[more]
A0A0M3LBE2_CLETE	3.1e-70	95.0	DNA-directed RNA polymerase su...	[more]
A0A0H3Y3A9_9MAGN	3.1e-70	95.0	DNA-directed RNA polymerase su...	[more]
A0A0H3Y388_9MAGN	3.1e-70	95.0	DNA-directed RNA polymerase su...	[more]
H6T9I2_9CARY	3.1e-70	95.7	DNA-directed RNA polymerase be...	[more]

BLAST of Spo19561.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
RPOC2_SPIOL	1.0e-74	99.3	DNA-directed RNA polymerase su...	[more]
RPOC2_CHLSC	2.4e-71	93.6	DNA-directed RNA polymerase su...	[more]
RPOC2_NANDO	3.1e-71	92.9	DNA-directed RNA polymerase su...	[more]
RPOC2_POPAL	5.3e-71	93.0	DNA-directed RNA polymerase su...	[more]
RPOC2_POPTR	5.3e-71	93.0	DNA-directed RNA polymerase su...	[more]

BLAST of Spo19561.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 1


Match Name	E-value	Identity	Description
ATCG00170.1	1.8e-69	90.8	DNA-directed RNA polymerase fa...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR007081	RNA polymerase Rpb1, domain 5	PFAM	PF04998	RNA_pol_Rpb1_5	coord: 121..163 score: 5.
None	No IPR available	PANTHER	PTHR19376	DNA-DIRECTED RNA POLYMERASE	coord: 31..163 score: 2.5
None	No IPR available	PANTHER	PTHR19376:SF38	DNA-DIRECTED RNA POLYMERASE SUBUNIT BETA''	coord: 31..163 score: 2.5
None	No IPR available	unknown	SSF64484	beta and beta-prime subunits of DNA dependent RNA-polymerase	coord: 34..163 score: 1.13

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006351	transcription, DNA-templated
biological_process	GO:0006144	purine nucleobase metabolic process
biological_process	GO:0006206	pyrimidine nucleobase metabolic process
biological_process	GO:0042254	ribosome biogenesis
biological_process	GO:0006412	translation
cellular_component	GO:0009507	chloroplast
cellular_component	GO:0005739	mitochondrion
cellular_component	GO:0005730	nucleolus
cellular_component	GO:0015935	small ribosomal subunit
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003899	DNA-directed 5'-3' RNA polymerase activity
molecular_function	GO:0008777	acetylornithine deacetylase activity
molecular_function	GO:0003735	structural constituent of ribosome