Spo20114.1 (mRNA)

Overview
NameSpo20114.1
TypemRNA
OrganismSpinacia oleracea (Spinach)
DescriptionBifunctional nuclease 1 (3.1.-.-)
LocationSpoScf_01064 : 62558 .. 67902 (+)
Sequence length1718
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAATATAAAAATAAAAATAAAAATAGGGGGCCTTGGAATCTCTTATCCAAAATTTATCAATTTTGCTCAAATAATTAATTTCGTCGGTCCATTTCTTGAAAACCCCCCTTTTCCACTTTCATCCCATTTATATTTTTTCAATCTCTCTCTTCAAAAATAAAATGATCAAACACCCATTTCGTATTTAGAATATTTGGAAATTGGGTCATTGTTTTTCTTACAGGTAATTGATCGTGGGGATCTAGTAGAATCTCAAGCTCTTTGATCATCAAAAAAATTCCAGATTCCTTCAAAGATTCTCTCTTTAACAGGTTTGATGCTAGAATCTGAATAATTATCCAATTTGTACTATGATTCAAGTGACTGTGATTTTGCAAAAGAACAATAATTTAAACAAATAAGGATACGGATAGAAAGTTGGATTTTTATTTCTAGATTTTGCATTATTATCATTTATCAATTATGCTTTGGGGATATGGAGGAATAAGTTTCTATTGTTACTGAGTCTACTGAACCTTACTGTTTTCTACTATTAGTTTTTAGTTTGATCTGAGGTCTTGGGTGTCATCCCCGACAGGCCAATACTTTGGGTTTATATTTGATCAGAAATTGTAATTAATTGAATAATACGACTTGTTGCATGAAGTATATTGTGTTTTTCTACTATTTGTTTTATTTGGCTGTTTTTTTGTATCAGATTGTATCTGTCAGACTTGTGGACCAGAAGTGAATCACCATAAAAAGTGTGAAAACTTAGTTGTATTAATCCCAAAATAGATTTCTGACTTAGAATTATTTTCTGGAATGCTTTAGAATAATTCTAAGTGAGAAATCTGTTTTGGGATTAATGAAGTGATTACCAGTATTAGTTCAGTAAGAAATGGTTTAGTGTTGGTGAATTTTCTGATGACCATTTAATTAACTGGTTTAGTGTTGAGAAGATTAACGAGTTGGGAGTAATTGTCTCACACATGGAAAAAAATAGAGAAAGATTGACTAATATATAAGATTGATGGGCTACTTCACCTATCACCAATTGGTTTTAGAATGGAACCCCATCAAGCTTATATGTGGTCAGTCTATTCTCATGTACGGGCTTGTATATGGCCCGATGAGTTTATCATTTAGTCATGATCCTTTTTTTGTTGTTATGACCTTGTTCCGATCATGTAGTTAAGTTGGTTGTGATGACTGATGGTTGTTGATCTTAGGACTTGAAGTAGGAGATGGGGTCATTACATGGACCAGTCGTTTGCCCAGCAATCCATTCAAAGCAGGCCAGTGTTTACCAAATACCAATTAATGTCTCTTCGATGAAGGATGACATGTTCAGAAGTACGTTTTGGGGTTATAAGGGTGTACGCCAACGAACTACTAACGCTGGCATTCTTACTCGCCCAATTAATATCCGAAGATTTGGTACAGTTCAATGCACTTTCAGTTCATCTTCCAATGGTAGTGATAGCAGAGCAGAGAATTTTAAGGAGAATGATGAGGATTATGTCAACTCCAGTGTAACTGAAGCTGGTAGGATCTACTCCCTTATATTTCATGAGTACAAACTAGCTCCATTAACTGGGTTCGATGCTTTTTATAATACTCCGTAGTATGCTTCTTCACATATTATTTTGAGCATACTCTTAATCAGTATTTTTTTTATTGCTTTATTGTAGTTGAGGTGAAAAGTGGTTCAGGTGGTTTCACAATTAAAATGAGAGATGGCAGGTTCTTAAGATGTGTCCATAACAGCCCTCAAGGCGGCCACCTTCCAGATTATGCACCACATCCGGCCATTGTGCTTAAGATGGAGGATGGAACTGGTCTTCTTCTTCCAATAATTGTTTGTATGTACATTGACCTCTTTGCAAGCATATCTGTGATTCTTTCAATTGTGTCCAAACCTAGTTGATAACCGCCAATTTGTATGTGTTCTTGCAGTAGAGATGCCTAGTGTGTTGCTCATGGCTGCTATCCGTAATGTTCATATTGTATGTGTATATTCTTCCGTGCGACTCAATTTTGTTATAAAGTCAATCAGCTACAATAGTCATAGTTGTAAAAATCTTATTACCTGATCTGATGAAACGTGCAGGCAAGGCCAACAATTTATCAGATCATGAAAGAGATGATTGAGAAGATGGGATACGCAGTAAGCGGGTTTTTAAAAAATTCCCCTCCCCAATGGTTCTTTGGTTGAAAGTGCAACTTTGTCTTTTTAATGTTTAAACCCCACGAAATATATTGCTCAGATACTTCTTTGCAGGTCAAACTTGTCCGTGTTACTAAGAGAGTCCATGAAGCATATTTTGCTCAGTTATATCTTACAAAGGCACGTGATATTATGTTCCACTTTTTTTCTTGTGAAGAACAATGGAATTGATCGTGAAAGGTCCTTTATTTCGATTTTTGTTATTTTTTCTTTGGTATAGATCGATAACGAAGACGAATGTATCAGCTTTGACCTTCGACCTTCAGATGCAATTAATATTGCTGTAAGATGCAAGGTATATTACATTTCCTTCCCATTTAGTTATTCCTGTGGCCCTTTGGGATGTTCTCTTCTTTCAACCAAAATAAATAAATAATAACAGTGGATTCTATTATTTTGGTAGATTCTGAGTTTTGGATCTTGCCAAATACTGCACTTTTTTTGGTGATTTTTGTTTCAAATTGCCAAATATTCTGGTTATGTCAGTTCAGGTTGATCGATATGATTATGTACATAGGTTACTGAGTGATTTGCCCATCTAGTATGTCATGACATTGATTGTCTCAGTTTGTCTTCTGATTACGGGACCTTGTGCAAAACAATTGCTGATGCACCTTTGTTCAGATTGTTAATTTGGTCAATCATAATTTTTTTCTCATACTGTTCATATGCTCCCCCAACTGTTGGTTTGATAGAAAGTTTTTAATACCAAAGGCTAAGTATTATACTATCCATAAGTTTCTAATTTCACTTGAATTTGACCCTCCTCTCATACGCTGATACGCATTCTCACTTCAACATGCTTTCTGTAGATAATCTACAAAGGGAAATAATACATTTAAAAAAGGGAAAACTGAACTAATGCTGCATCATTAGCTTCTAACTATAAAGTTCTATATGAACAAGAAAATGTAGACAGATCATAGATATACCAAAGTGTAACAATCCTTATGAGTTTAATAGCTTATAAGCTTTGCTCTGAAGCTCACATGTACCTACACAGGTTCCTATACAAGTCAACAGGGACTTGGCTCATAGTGATGGTATGAAGATTGTTGAATCTCCGAAGGCGACAATTGTGGGCCCTGCATCTGATGTCTTGTCATTTACAGAATTGGATCGGTAGGTTAATTTCAGTACTGTTTTTCCTGATGTTCCTTGCTTTACTTCTTACTTCTAGCTGTATAACCCATGCCCTTCTCCTTGAAGAAATGGCGAGTTCCTTGAGCCTAGGAACTGGATTTCTGATGGAAGCACACTGTTTTATGACTTTTATCCTAGTTACATGTATGCAGTACTAGGTTTTCCACCCTATGGACAGCCTGTTTATTGTTTAATATCTGTTCCAGTCACTTTTTGGGTTTTGATATTATCCTGTTACACTGCAACCATTTAGCAGGTCAAGGATATCTGCTCAATGAGCAGAGAGTGTTGTGAAATTTGTAGGATACGTTCATAGTTGATTTCCTCTTTTTAACCTTTCGTGCTCATGCCTATAAATGATTGGAACTTAAGTCACTTAAGATGAGTAGGTGGATGAAAGAACTTCTTGTATGAATGTCCTGTTTCTGTTCTTTCATCTTTGAGATTGTAACGTTTGTTCTAGACTGTGGCTTGTTCATTTCTTTGACAGTTGACATGAAGAAAACTAGCTATTATCATCTCAGTCTCCCTCCATCTTGACTCGAGAACTAAAAAATTTAGAATAATACTTGGATTTTACCAAAGTTTTTCTTTGTCATCTTGGAAGAACCCGAAACACAGTGCTTTCAGTTCTCATCCTATCTCACTATGGTTGTGTTTTGAATTGTGCTTCTGCTTTAGTTTTGTTGCTTTACACTAACATTTTGCTGAACACTTGAAAGTGAATAGTGAATCAATGGAGAAAAATGGAGGCGCTAAAGCATATTGCACATGTTTAATTTAAATAATTTATTTCCGTGTTGTACTGAGGTTCCTTCAGAGTAGAAAAACACTTTAAAAGTTGTTGGTCTTTCTTATTTATATTTTCTACTCCGTCCTTTTTATGGGAGGAAAGATGAAGAAGAAATTAGGAAAGTGTTTTTGATTACATTATTACTGTGTTAATGAAATTGACATGGTTTCTTATACAGTACTTGTGACTGACTTGATCTAATATGTGGAACACAGTGTATAATGATATTTGCTTGGTTTTGACTCAGGCCTAATGGTAAGCCGTGTATAGAGACAACAGAATTTAACCTGCTGCAAAACATGCTTAATGCTGCGTTGGAGGAGCGCTATCAAGAAGCAGGTATGTATGATATACCTCTTGTTTCTGCGTTCTGCTGTTTAGCATTGGTTTTCCAGTTTGTTAAGATTAAGTGCTTCTCTTGTACTACGTATGTTCTGAAGTGAAAGTCTATTTACACTCATGGTTACTGTGCAGCTCAATGGAGGGACCAACTAACCCAATTCCGAGCTAAGAGGAACTGGGCATGACAGGTGACGGATTACCTTTATTTACAATACGCTACTTAATTAATTCCCTTGATACTGTTGTGTTGTAAGTTATTTGGTATTTGTTGTTGCAGGAAGCAAATATGGTACATTGTAAATAAAATTTATGAAGCTGAAGATTATGCTGGCTCCGTTGCTAGGTTTCTTTGTTAATTAATGTTTGTGATCATATTTGTGAATAGTAGCGTAGATGATGAACAATCTCATTCCAGTCCGAATCCGAATAGAACATACTTGCTATGCTTAGTATGCTTCAGGCCGGCTGCCGTAGTTCGATATTGTATACCAATATGAATGTTGAATGATGTATCACTATCGCCTATGGTATATGACCTTCCCGGGGTAAACTGGAAATATGTACTTTCAAACCATCTACTGTGAATATGCGATGTTTTAATTATTAACTAGTGTGTGACCCGGGCGGATGCTCCGGTTGCTACTTTGAATAATTAAACTTGGATATTTTTCTCAATTCAGCTCAATATTTGACCAAAGTACGTGTAGTCCGTAAAAGTGAAAAGATTCATTAAGTTCTTTAACTACACATGTACATGCGTCAATTATCATGCCAATTAATTTTTCAATTAGATCATTTTATAATTCGGTTAATTTATTAATGGAACGAAGTGCTTCAAATTAATAAGATATATGCAAG

mRNA sequence

AAATATAAAAATAAAAATAAAAATAGGGGGCCTTGGAATCTCTTATCCAAAATTTATCAATTTTGCTCAAATAATTAATTTCGTCGGTCCATTTCTTGAAAACCCCCCTTTTCCACTTTCATCCCATTTATATTTTTTCAATCTCTCTCTTCAAAAATAAAATGATCAAACACCCATTTCGTATTTAGAATATTTGGAAATTGGGTCATTGTTTTTCTTACAGGTAATTGATCGTGGGGATCTAGTAGAATCTCAAGCTCTTTGATCATCAAAAAAATTCCAGATTCCTTCAAAGATTCTCTCTTTAACAGGACTTGAAGTAGGAGATGGGGTCATTACATGGACCAGTCGTTTGCCCAGCAATCCATTCAAAGCAGGCCAGTGTTTACCAAATACCAATTAATGTCTCTTCGATGAAGGATGACATGTTCAGAAGTACGTTTTGGGGTTATAAGGGTGTACGCCAACGAACTACTAACGCTGGCATTCTTACTCGCCCAATTAATATCCGAAGATTTGGTACAGTTCAATGCACTTTCAGTTCATCTTCCAATGGTAGTGATAGCAGAGCAGAGAATTTTAAGGAGAATGATGAGGATTATGTCAACTCCAGTGTAACTGAAGCTGTTGAGGTGAAAAGTGGTTCAGGTGGTTTCACAATTAAAATGAGAGATGGCAGGTTCTTAAGATGTGTCCATAACAGCCCTCAAGGCGGCCACCTTCCAGATTATGCACCACATCCGGCCATTGTGCTTAAGATGGAGGATGGAACTGGTCTTCTTCTTCCAATAATTGCAAGGCCAACAATTTATCAGATCATGAAAGAGATGATTGAGAAGATGGGATACGCAATCGATAACGAAGACGAATGTATCAGCTTTGACCTTCGACCTTCAGATGCAATTAATATTGCTGTAAGATGCAAGGTTCCTATACAAGTCAACAGGGACTTGGCTCATAGTGATGGTATGAAGATTGTTGAATCTCCGAAGGCGACAATTGTGGGCCCTGCATCTGATGTCTTGTCATTTACAGAATTGGATCGGCCTAATGGTAAGCCGTGTATAGAGACAACAGAATTTAACCTGCTGCAAAACATGCTTAATGCTGCGTTGGAGGAGCGCTATCAAGAAGCAGGAAGCAAATATGGTACATTGTAAATAAAATTTATGAAGCTGAAGATTATGCTGGCTCCGTTGCTAGGTTTCTTTGTTAATTAATGTTTGTGATCATATTTGTGAATAGTAGCGTAGATGATGAACAATCTCATTCCAGTCCGAATCCGAATAGAACATACTTGCTATGCTTAGTATGCTTCAGGCCGGCTGCCGTAGTTCGATATTGTATACCAATATGAATGTTGAATGATGTATCACTATCGCCTATGGTATATGACCTTCCCGGGGTAAACTGGAAATATGTACTTTCAAACCATCTACTGTGAATATGCGATGTTTTAATTATTAACTAGTGTGTGACCCGGGCGGATGCTCCGGTTGCTACTTTGAATAATTAAACTTGGATATTTTTCTCAATTCAGCTCAATATTTGACCAAAGTACGTGTAGTCCGTAAAAGTGAAAAGATTCATTAAGTTCTTTAACTACACATGTACATGCGTCAATTATCATGCCAATTAATTTTTCAATTAGATCATTTTATAATTCGGTTAATTTATTAATGGAACGAAGTGCTTCAAATTAATAAGATATATGCAAG

Coding sequence (CDS)

ATGGGGTCATTACATGGACCAGTCGTTTGCCCAGCAATCCATTCAAAGCAGGCCAGTGTTTACCAAATACCAATTAATGTCTCTTCGATGAAGGATGACATGTTCAGAAGTACGTTTTGGGGTTATAAGGGTGTACGCCAACGAACTACTAACGCTGGCATTCTTACTCGCCCAATTAATATCCGAAGATTTGGTACAGTTCAATGCACTTTCAGTTCATCTTCCAATGGTAGTGATAGCAGAGCAGAGAATTTTAAGGAGAATGATGAGGATTATGTCAACTCCAGTGTAACTGAAGCTGTTGAGGTGAAAAGTGGTTCAGGTGGTTTCACAATTAAAATGAGAGATGGCAGGTTCTTAAGATGTGTCCATAACAGCCCTCAAGGCGGCCACCTTCCAGATTATGCACCACATCCGGCCATTGTGCTTAAGATGGAGGATGGAACTGGTCTTCTTCTTCCAATAATTGCAAGGCCAACAATTTATCAGATCATGAAAGAGATGATTGAGAAGATGGGATACGCAATCGATAACGAAGACGAATGTATCAGCTTTGACCTTCGACCTTCAGATGCAATTAATATTGCTGTAAGATGCAAGGTTCCTATACAAGTCAACAGGGACTTGGCTCATAGTGATGGTATGAAGATTGTTGAATCTCCGAAGGCGACAATTGTGGGCCCTGCATCTGATGTCTTGTCATTTACAGAATTGGATCGGCCTAATGGTAAGCCGTGTATAGAGACAACAGAATTTAACCTGCTGCAAAACATGCTTAATGCTGCGTTGGAGGAGCGCTATCAAGAAGCAGGAAGCAAATATGGTACATTGTAA

Protein sequence

MGSLHGPVVCPAIHSKQASVYQIPINVSSMKDDMFRSTFWGYKGVRQRTTNAGILTRPINIRRFGTVQCTFSSSSNGSDSRAENFKENDEDYVNSSVTEAVEVKSGSGGFTIKMRDGRFLRCVHNSPQGGHLPDYAPHPAIVLKMEDGTGLLLPIIARPTIYQIMKEMIEKMGYAIDNEDECISFDLRPSDAINIAVRCKVPIQVNRDLAHSDGMKIVESPKATIVGPASDVLSFTELDRPNGKPCIETTEFNLLQNMLNAALEERYQEAGSKYGTL
Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Spo20114Spo20114gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Spo20114.1Spo20114.1-proteinpolypeptide


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Spo20114.1.exon.1Spo20114.1.exon.1exon
Spo20114.1.exon.2Spo20114.1.exon.2exon
Spo20114.1.exon.3Spo20114.1.exon.3exon
Spo20114.1.exon.4Spo20114.1.exon.4exon
Spo20114.1.exon.5Spo20114.1.exon.5exon
Spo20114.1.exon.6Spo20114.1.exon.6exon
Spo20114.1.exon.7Spo20114.1.exon.7exon
Spo20114.1.exon.8Spo20114.1.exon.8exon


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Spo20114.1.utr5p.1Spo20114.1.utr5p.1five_prime_UTR
Spo20114.1.utr5p.2Spo20114.1.utr5p.2five_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Spo20114.1.CDS.1Spo20114.1.CDS.1CDS
Spo20114.1.CDS.2Spo20114.1.CDS.2CDS
Spo20114.1.CDS.3Spo20114.1.CDS.3CDS
Spo20114.1.CDS.4Spo20114.1.CDS.4CDS
Spo20114.1.CDS.5Spo20114.1.CDS.5CDS
Spo20114.1.CDS.6Spo20114.1.CDS.6CDS
Spo20114.1.CDS.7Spo20114.1.CDS.7CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Spo20114.1.utr3p.1Spo20114.1.utr3p.1three_prime_UTR


Homology
[Close]
BLAST of Spo20114.1 vs. NCBI nr
Match: gi|902235563|gb|KNA23976.1| (hypothetical protein SOVF_020250 [Spinacia oleracea])

HSP 1 Score: 525.0 bits (1351), Expect = 7.900e-146
Identity = 270/310 (87.10%), Postives = 270/310 (87.10%), Query Frame = 1 

		  

Query: 1   MGSLHGPVVCPAIHSKQASVYQIPINVSSMKDDMFRSTFWGYKGVRQRTTNAGILTRPIN 60
           MGSLHGPVVCPAIHSKQASVYQIPINVSSMKDDMFRSTFWGYKGVRQRTTNAGILTRPIN
Sbjct: 1   MGSLHGPVVCPAIHSKQASVYQIPINVSSMKDDMFRSTFWGYKGVRQRTTNAGILTRPIN 60

Query: 61  IRRFGTVQCTFSSSSNGSDSRAENFKENDEDYVNSSVTEAVEVKSGSGGFTIKMRDGRFL 120
           IRRFGTVQCTFSSSSNGSDSRAENFKENDEDYVNSSVTEAVEVKSGSGGFTIKMRDGRFL
Sbjct: 61  IRRFGTVQCTFSSSSNGSDSRAENFKENDEDYVNSSVTEAVEVKSGSGGFTIKMRDGRFL 120

Query: 121 RCVHNSPQGGHLPDYAPHPAIVLKMEDGTGLLLPI------------------IARPTIY 180
           RCVHNSPQGGHLPDYAPHPAIVLKMEDGTGLLLPI                  IARPTIY
Sbjct: 121 RCVHNSPQGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAIRNVHIARPTIY 180

Query: 181 QIMKEMIEKMGYA----------------------IDNEDECISFDLRPSDAINIAVRCK 240
           QIMKEMIEKMGYA                      IDNEDECISFDLRPSDAINIAVRCK
Sbjct: 181 QIMKEMIEKMGYAVKLVRVTKRVHEAYFAQLYLTKIDNEDECISFDLRPSDAINIAVRCK 240

Query: 241 VPIQVNRDLAHSDGMKIVESPKATIVGPASDVLSFTELDRPNGKPCIETTEFNLLQNMLN 271
           VPIQVNRDLAHSDGMKIVESPKATIVGPASDVLSFTELDRPNGKPCIETTEFNLLQNMLN
Sbjct: 241 VPIQVNRDLAHSDGMKIVESPKATIVGPASDVLSFTELDRPNGKPCIETTEFNLLQNMLN 300
[Close]
BLAST of Spo20114.1 vs. NCBI nr
Match: gi|731312202|ref|XP_010673061.1| (PREDICTED: bifunctional nuclease 1 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 450.3 bits (1157), Expect = 2.500e-123
Identity = 229/310 (73.87%), Postives = 247/310 (79.68%), Query Frame = 1 

		  

Query: 1   MGSLHGPVVCPAIHSKQASVYQIPINVSSMKDDMFRSTFWGYKGVRQRTTNAGILTRPIN 60
           MGSLHGPVVCP I +KQ  VYQ+P N S MK+ M RSTFWGYK VRQR +NAG+L  P N
Sbjct: 1   MGSLHGPVVCPTIRAKQVGVYQVPTNSSLMKNAMLRSTFWGYKSVRQRVSNAGMLASPTN 60

Query: 61  IRRFGTVQCTFSSSSNGSDSRAENFKENDEDYVNSSVTEAVEVKSGSGGFTIKMRDGRFL 120
           IRR G V CTFSSSSNGS SRAENFKENDEDYVNSSVTEAVEVKSGS GFTIKMRDG+++
Sbjct: 61  IRRLGLVHCTFSSSSNGSGSRAENFKENDEDYVNSSVTEAVEVKSGSDGFTIKMRDGKYI 120

Query: 121 RCVHNSPQGGHLPDYAPHPAIVLKMEDGTGLLLPI------------------IARPTIY 180
           +C+HNSPQGGHLPDYAPHPAIVLKMEDGTGLLLPI                  IARPT+Y
Sbjct: 121 KCIHNSPQGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAIRNVHIARPTMY 180

Query: 181 QIMKEMIEKMGYA----------------------IDNEDECISFDLRPSDAINIAVRCK 240
           Q+MKEMIEKMGYA                      +DNEDECISFDLRPSDAINIAVRCK
Sbjct: 181 QVMKEMIEKMGYAVKLVRVTKRVHEAYFAQMFLTKVDNEDECISFDLRPSDAINIAVRCK 240

Query: 241 VPIQVNRDLAHSDGMKIVESPKATIVGPASDVLSFTELDRPNGKPCIETTEFNLLQNMLN 271
           VPIQVNR LAHSDGMK+VES KATIVGPASD+L FTELDRPNGKPCIETTEFNLL+NML+
Sbjct: 241 VPIQVNRSLAHSDGMKVVESVKATIVGPASDLL-FTELDRPNGKPCIETTEFNLLRNMLS 300
[Close]
BLAST of Spo20114.1 vs. NCBI nr
Match: gi|147842247|emb|CAN76215.1| (hypothetical protein VITISV_009514 [Vitis vinifera])

HSP 1 Score: 369.0 bits (946), Expect = 7.200e-99
Identity = 182/306 (59.48%), Postives = 226/306 (73.86%), Query Frame = 1 

		  

Query: 1   MGSLHGPVVCPAIHSKQASVYQIPINVSSMKDDMFRSTFWGYKGVRQRTTNAGILTRPIN 60
           MG+L GPV+CP + +K+A VY +P+++   K  +FRS FWG++G     T AG +   ++
Sbjct: 1   MGTLQGPVICPTVRAKEAGVYTLPVSLPLKKTKIFRSGFWGFRGNCGGRTKAGXVPHQLH 60

Query: 61  IRRFGTVQCTFSSSSNGSDSRAENFKENDEDYVNSSVTEAVEVKSGSGGFTIKMRDGRFL 120
            R+  T+QC+FSSSS+G+ S AENF ENDEDYVNSSV EAVEVKSG+ GF IKMRDGR L
Sbjct: 61  TRKCKTLQCSFSSSSDGNGSMAENFNENDEDYVNSSVVEAVEVKSGADGFMIKMRDGRHL 120

Query: 121 RCVHNSPQGGHLPDYAPHPAIVLKMEDGTGLLLPIIARPTIYQIMKEMIEKMGYAID--- 180
           RCVHN+PQGGH+PDYAPHPAIVLKMEDGTGLLLPIIARPT+YQ+MKEMI+KMGYA+    
Sbjct: 121 RCVHNNPQGGHMPDYAPHPAIVLKMEDGTGLLLPIIARPTLYQVMKEMIDKMGYAVKLVR 180

Query: 181 ---------------------------------NEDECISFDLRPSDAINIAVRCKVPIQ 240
                                            NE E +SFDLRPSDAINIAVRCKVPIQ
Sbjct: 181 VTKRVHEAYFAQLYLTKAYSCCTPCSLIDCLVGNEKESVSFDLRPSDAINIAVRCKVPIQ 240

Query: 241 VNRDLAHSDGMKIVESPKATIVGPASDVLSFTELDRPNGKPCIETTEFNLLQNMLNAALE 271
           VN+ LA+SDGM+++ES K ++   +SD L FTELDRP+G+PCIET EF+L++NML AA+E
Sbjct: 241 VNKYLAYSDGMRVIESAKVSVQASSSDGLLFTELDRPSGQPCIETKEFDLVRNMLIAAVE 300
[Close]
BLAST of Spo20114.1 vs. NCBI nr
Match: gi|641865955|gb|KDO84640.1| (hypothetical protein CISIN_1g019109mg [Citrus sinensis])

HSP 1 Score: 364.8 bits (935), Expect = 1.400e-97
Identity = 187/312 (59.94%), Postives = 221/312 (70.83%), Query Frame = 1 

		  

Query: 1   MGSLHGPVVCPAIHSKQASVYQ-IPINVSSMKDDMFRSTFWGYKGVRQRTTNAGILTRPI 60
           MGSL GPV CPA+ ++Q  +Y  +P     MK   FRS  WG+KG+    T  G+ TR  
Sbjct: 1   MGSLQGPVFCPAVRARQGGLYNTVPSTGPVMKARFFRSELWGFKGLTAVKTKVGVFTRQQ 60

Query: 61  NIRRFGTVQCTFSSSSNGSDSRAENFKENDEDYVNSSVTEAVEVKSGSGGFTIKMRDGRF 120
           N R+   VQCTFSSSSNG+ S AENF ENDED+VNSSV EAVEVKSG+ GF IKMRDGR 
Sbjct: 61  NARKCKIVQCTFSSSSNGNGSMAENFNENDEDFVNSSVVEAVEVKSGADGFMIKMRDGRH 120

Query: 121 LRCVHNSPQGGHLPDYAPHPAIVLKMEDGTGLLLPI------------------IARPTI 180
           LRCVHN+PQGGHLPDYAPHPAIVLKMEDGTGLLLPI                  IARPT+
Sbjct: 121 LRCVHNNPQGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAMRNVQIARPTL 180

Query: 181 YQIMKEMIEKMGY----------------------AIDNEDECISFDLRPSDAINIAVRC 240
           YQ++KEMIEKMGY                       + NE EC+SFDLRPSDAINIAVRC
Sbjct: 181 YQVVKEMIEKMGYEVRLVRVTKRVHEAYFAQLYLTKVGNETECVSFDLRPSDAINIAVRC 240

Query: 241 KVPIQVNRDLAHSDGMKIVESPKATIVGPASDVLSFTELDRPNGKPCIETTEFNLLQNML 272
           KVPIQVN+ LA+SDGM+++ES K +   P SD L FTELD+P+G+PC++T EFNL++NML
Sbjct: 241 KVPIQVNKYLAYSDGMRVIESGKLSTHSPGSDGLLFTELDKPSGQPCLDTKEFNLVRNML 300
[Close]
BLAST of Spo20114.1 vs. NCBI nr
Match: gi|568839128|ref|XP_006473546.1| (PREDICTED: bifunctional nuclease 1 [Citrus sinensis])

HSP 1 Score: 363.6 bits (932), Expect = 3.000e-97
Identity = 187/311 (60.13%), Postives = 221/311 (71.06%), Query Frame = 1 

		  

Query: 1   MGSLHGPVVCPAIHSKQASVYQ-IPINVSSMKDDMFRSTFWGYKGVRQRTTNAGILTRPI 60
           MGSL GPV CPA+ ++Q  +Y  +P     MK  +FRS  WG+KG+    T  G+ TR  
Sbjct: 1   MGSLQGPVFCPAVRARQGGLYNTVPSTGPVMKARLFRSELWGFKGLTAVKTKVGVFTRQQ 60

Query: 61  NIRRFGTVQCTFSSSSNGSDSRAENFKENDEDYVNSSVTEAVEVKSGSGGFTIKMRDGRF 120
           N R+   VQCTFSSSSNG+ S AENF ENDED+VNSSV EAVEVKSG+ GF IKMRDGR 
Sbjct: 61  NARKCKIVQCTFSSSSNGNGSMAENFNENDEDFVNSSVVEAVEVKSGADGFMIKMRDGRH 120

Query: 121 LRCVHNSPQGGHLPDYAPHPAIVLKMEDGTGLLLPI------------------IARPTI 180
           LRCVHN+PQGGHLPDYAPHPAIVLKMEDGTGLLLPI                  IARPT+
Sbjct: 121 LRCVHNNPQGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAMRNVQIARPTL 180

Query: 181 YQIMKEMIEKMGY----------------------AIDNEDECISFDLRPSDAINIAVRC 240
           YQ++KEMIEKMGY                       + NE EC+SFDLRPSDAINIAVRC
Sbjct: 181 YQVVKEMIEKMGYEVRLVRVTKRVHEAYFAQLYLTKVGNETECVSFDLRPSDAINIAVRC 240

Query: 241 KVPIQVNRDLAHSDGMKIVESPKATIVGPASDVLSFTELDRPNGKPCIETTEFNLLQNML 271
           KVPIQVN+ LA+SDGM++VES K +   P SD L FTELD+P+G+PC++T EFNL++NML
Sbjct: 241 KVPIQVNKYLAYSDGMRVVESGKLSTHSPGSDGLLFTELDKPSGQPCLDTKEFNLVRNML 300
[Close]
BLAST of Spo20114.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RWT8_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_020250 PE=4 SV=1)

HSP 1 Score: 525.0 bits (1351), Expect = 5.500e-146
Identity = 270/310 (87.10%), Postives = 270/310 (87.10%), Query Frame = 1 

		  

Query: 1   MGSLHGPVVCPAIHSKQASVYQIPINVSSMKDDMFRSTFWGYKGVRQRTTNAGILTRPIN 60
           MGSLHGPVVCPAIHSKQASVYQIPINVSSMKDDMFRSTFWGYKGVRQRTTNAGILTRPIN
Sbjct: 1   MGSLHGPVVCPAIHSKQASVYQIPINVSSMKDDMFRSTFWGYKGVRQRTTNAGILTRPIN 60

Query: 61  IRRFGTVQCTFSSSSNGSDSRAENFKENDEDYVNSSVTEAVEVKSGSGGFTIKMRDGRFL 120
           IRRFGTVQCTFSSSSNGSDSRAENFKENDEDYVNSSVTEAVEVKSGSGGFTIKMRDGRFL
Sbjct: 61  IRRFGTVQCTFSSSSNGSDSRAENFKENDEDYVNSSVTEAVEVKSGSGGFTIKMRDGRFL 120

Query: 121 RCVHNSPQGGHLPDYAPHPAIVLKMEDGTGLLLPI------------------IARPTIY 180
           RCVHNSPQGGHLPDYAPHPAIVLKMEDGTGLLLPI                  IARPTIY
Sbjct: 121 RCVHNSPQGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAIRNVHIARPTIY 180

Query: 181 QIMKEMIEKMGYA----------------------IDNEDECISFDLRPSDAINIAVRCK 240
           QIMKEMIEKMGYA                      IDNEDECISFDLRPSDAINIAVRCK
Sbjct: 181 QIMKEMIEKMGYAVKLVRVTKRVHEAYFAQLYLTKIDNEDECISFDLRPSDAINIAVRCK 240

Query: 241 VPIQVNRDLAHSDGMKIVESPKATIVGPASDVLSFTELDRPNGKPCIETTEFNLLQNMLN 271
           VPIQVNRDLAHSDGMKIVESPKATIVGPASDVLSFTELDRPNGKPCIETTEFNLLQNMLN
Sbjct: 241 VPIQVNRDLAHSDGMKIVESPKATIVGPASDVLSFTELDRPNGKPCIETTEFNLLQNMLN 300
[Close]
BLAST of Spo20114.1 vs. UniProtKB/TrEMBL
Match: A0A0J8D8Y6_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_1g007020 PE=4 SV=1)

HSP 1 Score: 450.3 bits (1157), Expect = 1.700e-123
Identity = 229/310 (73.87%), Postives = 247/310 (79.68%), Query Frame = 1 

		  

Query: 1   MGSLHGPVVCPAIHSKQASVYQIPINVSSMKDDMFRSTFWGYKGVRQRTTNAGILTRPIN 60
           MGSLHGPVVCP I +KQ  VYQ+P N S MK+ M RSTFWGYK VRQR +NAG+L  P N
Sbjct: 1   MGSLHGPVVCPTIRAKQVGVYQVPTNSSLMKNAMLRSTFWGYKSVRQRVSNAGMLASPTN 60

Query: 61  IRRFGTVQCTFSSSSNGSDSRAENFKENDEDYVNSSVTEAVEVKSGSGGFTIKMRDGRFL 120
           IRR G V CTFSSSSNGS SRAENFKENDEDYVNSSVTEAVEVKSGS GFTIKMRDG+++
Sbjct: 61  IRRLGLVHCTFSSSSNGSGSRAENFKENDEDYVNSSVTEAVEVKSGSDGFTIKMRDGKYI 120

Query: 121 RCVHNSPQGGHLPDYAPHPAIVLKMEDGTGLLLPI------------------IARPTIY 180
           +C+HNSPQGGHLPDYAPHPAIVLKMEDGTGLLLPI                  IARPT+Y
Sbjct: 121 KCIHNSPQGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAIRNVHIARPTMY 180

Query: 181 QIMKEMIEKMGYA----------------------IDNEDECISFDLRPSDAINIAVRCK 240
           Q+MKEMIEKMGYA                      +DNEDECISFDLRPSDAINIAVRCK
Sbjct: 181 QVMKEMIEKMGYAVKLVRVTKRVHEAYFAQMFLTKVDNEDECISFDLRPSDAINIAVRCK 240

Query: 241 VPIQVNRDLAHSDGMKIVESPKATIVGPASDVLSFTELDRPNGKPCIETTEFNLLQNMLN 271
           VPIQVNR LAHSDGMK+VES KATIVGPASD+L FTELDRPNGKPCIETTEFNLL+NML+
Sbjct: 241 VPIQVNRSLAHSDGMKVVESVKATIVGPASDLL-FTELDRPNGKPCIETTEFNLLRNMLS 300
[Close]
BLAST of Spo20114.1 vs. UniProtKB/TrEMBL
Match: A5AZ38_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VITISV_009514 PE=4 SV=1)

HSP 1 Score: 369.0 bits (946), Expect = 5.000e-99
Identity = 182/306 (59.48%), Postives = 226/306 (73.86%), Query Frame = 1 

		  

Query: 1   MGSLHGPVVCPAIHSKQASVYQIPINVSSMKDDMFRSTFWGYKGVRQRTTNAGILTRPIN 60
           MG+L GPV+CP + +K+A VY +P+++   K  +FRS FWG++G     T AG +   ++
Sbjct: 1   MGTLQGPVICPTVRAKEAGVYTLPVSLPLKKTKIFRSGFWGFRGNCGGRTKAGXVPHQLH 60

Query: 61  IRRFGTVQCTFSSSSNGSDSRAENFKENDEDYVNSSVTEAVEVKSGSGGFTIKMRDGRFL 120
            R+  T+QC+FSSSS+G+ S AENF ENDEDYVNSSV EAVEVKSG+ GF IKMRDGR L
Sbjct: 61  TRKCKTLQCSFSSSSDGNGSMAENFNENDEDYVNSSVVEAVEVKSGADGFMIKMRDGRHL 120

Query: 121 RCVHNSPQGGHLPDYAPHPAIVLKMEDGTGLLLPIIARPTIYQIMKEMIEKMGYAID--- 180
           RCVHN+PQGGH+PDYAPHPAIVLKMEDGTGLLLPIIARPT+YQ+MKEMI+KMGYA+    
Sbjct: 121 RCVHNNPQGGHMPDYAPHPAIVLKMEDGTGLLLPIIARPTLYQVMKEMIDKMGYAVKLVR 180

Query: 181 ---------------------------------NEDECISFDLRPSDAINIAVRCKVPIQ 240
                                            NE E +SFDLRPSDAINIAVRCKVPIQ
Sbjct: 181 VTKRVHEAYFAQLYLTKAYSCCTPCSLIDCLVGNEKESVSFDLRPSDAINIAVRCKVPIQ 240

Query: 241 VNRDLAHSDGMKIVESPKATIVGPASDVLSFTELDRPNGKPCIETTEFNLLQNMLNAALE 271
           VN+ LA+SDGM+++ES K ++   +SD L FTELDRP+G+PCIET EF+L++NML AA+E
Sbjct: 241 VNKYLAYSDGMRVIESAKVSVQASSSDGLLFTELDRPSGQPCIETKEFDLVRNMLIAAVE 300
[Close]
BLAST of Spo20114.1 vs. UniProtKB/TrEMBL
Match: A0A067H158_CITSI (Uncharacterized protein OS=Citrus sinensis GN=CISIN_1g019109mg PE=4 SV=1)

HSP 1 Score: 364.8 bits (935), Expect = 9.500e-98
Identity = 187/312 (59.94%), Postives = 221/312 (70.83%), Query Frame = 1 

		  

Query: 1   MGSLHGPVVCPAIHSKQASVYQ-IPINVSSMKDDMFRSTFWGYKGVRQRTTNAGILTRPI 60
           MGSL GPV CPA+ ++Q  +Y  +P     MK   FRS  WG+KG+    T  G+ TR  
Sbjct: 1   MGSLQGPVFCPAVRARQGGLYNTVPSTGPVMKARFFRSELWGFKGLTAVKTKVGVFTRQQ 60

Query: 61  NIRRFGTVQCTFSSSSNGSDSRAENFKENDEDYVNSSVTEAVEVKSGSGGFTIKMRDGRF 120
           N R+   VQCTFSSSSNG+ S AENF ENDED+VNSSV EAVEVKSG+ GF IKMRDGR 
Sbjct: 61  NARKCKIVQCTFSSSSNGNGSMAENFNENDEDFVNSSVVEAVEVKSGADGFMIKMRDGRH 120

Query: 121 LRCVHNSPQGGHLPDYAPHPAIVLKMEDGTGLLLPI------------------IARPTI 180
           LRCVHN+PQGGHLPDYAPHPAIVLKMEDGTGLLLPI                  IARPT+
Sbjct: 121 LRCVHNNPQGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAMRNVQIARPTL 180

Query: 181 YQIMKEMIEKMGY----------------------AIDNEDECISFDLRPSDAINIAVRC 240
           YQ++KEMIEKMGY                       + NE EC+SFDLRPSDAINIAVRC
Sbjct: 181 YQVVKEMIEKMGYEVRLVRVTKRVHEAYFAQLYLTKVGNETECVSFDLRPSDAINIAVRC 240

Query: 241 KVPIQVNRDLAHSDGMKIVESPKATIVGPASDVLSFTELDRPNGKPCIETTEFNLLQNML 272
           KVPIQVN+ LA+SDGM+++ES K +   P SD L FTELD+P+G+PC++T EFNL++NML
Sbjct: 241 KVPIQVNKYLAYSDGMRVIESGKLSTHSPGSDGLLFTELDKPSGQPCLDTKEFNLVRNML 300
[Close]
BLAST of Spo20114.1 vs. UniProtKB/TrEMBL
Match: E0CQH3_VITVI (Putative uncharacterized protein OS=Vitis vinifera GN=VIT_18s0001g12960 PE=4 SV=1)

HSP 1 Score: 362.8 bits (930), Expect = 3.600e-97
Identity = 182/310 (58.71%), Postives = 226/310 (72.90%), Query Frame = 1 

		  

Query: 1   MGSLHGPVVCPAIHSKQASVYQIPINVSSMKDDMFRSTFWGYKGVRQRTTNAGILTRPIN 60
           MG+L GPV+CP + +K+A VY +P+++   K  +FRS FWG++G     T AG +   ++
Sbjct: 29  MGTLQGPVICPTVRAKEAGVYTLPVSLPLKKTKIFRSGFWGFRGNCGGRTKAGSVPHQLH 88

Query: 61  IRRFGTVQCTFSSSSNGSDSRAENFKENDEDYVNSSVTEAVEVKSGSGGFTIKMRDGRFL 120
            R+  T+QC+FSSSS+G+ S AENF ENDEDYVNSSV EAVEVKSG+ GF IKMRDGR L
Sbjct: 89  TRKCKTLQCSFSSSSDGNGSMAENFNENDEDYVNSSVVEAVEVKSGADGFMIKMRDGRHL 148

Query: 121 RCVHNSPQGGHLPDYAPHPAIVLKMEDGTGLLLPI------------------IARPTIY 180
           RCVHN+PQGGH+PDYAPHPAIVLKMEDGTGLLLPI                  IARPT+Y
Sbjct: 149 RCVHNNPQGGHMPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAVRNVQIARPTLY 208

Query: 181 QIMKEMIEKMGYA----------------------IDNEDECISFDLRPSDAINIAVRCK 240
           Q+MKEMI+KMGYA                      + NE E +SFDLRPSDAINIAVRCK
Sbjct: 209 QVMKEMIDKMGYAVKLVRVTKRVHEAYFAQLYLTKVGNEKESVSFDLRPSDAINIAVRCK 268

Query: 241 VPIQVNRDLAHSDGMKIVESPKATIVGPASDVLSFTELDRPNGKPCIETTEFNLLQNMLN 271
           VPIQVN+ LA+SDGM+++ES K ++   +SD L FTELDRP+G+PCIET EF+L++NML 
Sbjct: 269 VPIQVNKYLAYSDGMRVIESAKVSVQASSSDGLLFTELDRPSGQPCIETKEFDLVRNMLI 328
[Close]
BLAST of Spo20114.1 vs. ExPASy Swiss-Prot
Match: BBD1_ARATH (Bifunctional nuclease 1 OS=Arabidopsis thaliana GN=BBD1 PE=2 SV=1)

HSP 1 Score: 320.1 bits (819), Expect = 2.400e-86
Identity = 171/312 (54.81%), Postives = 206/312 (66.03%), Query Frame = 1 

		  

Query: 1   MGSLHGPVVCPAIHSKQASVYQIPINVSSMKDDMFRSTFWGY--KGVRQRTTNAGILTRP 60
           M S+  PVVCPAI  +Q       +N + +K    RS FWG   KGV+ + T   I  R 
Sbjct: 1   MRSVQAPVVCPAIRPRQVGACASLVNYTGLKP---RSQFWGNRTKGVKSQGTTTTITLRL 60

Query: 61  INIRRFGTVQCTFSSSSNGSDSRAENFKENDEDYVNSSVTEAVEVKSGSGGFTIKMRDGR 120
            N     +++C FSS S+G+ S AENF ENDE+YVNSSV EAVEVKSG+ GF +KMRDGR
Sbjct: 61  CN----KSIKCVFSSHSDGNGSTAENFNENDEEYVNSSVVEAVEVKSGADGFMVKMRDGR 120

Query: 121 FLRCVHNSPQGGHLPDYAPHPAIVLKMEDGTGLLLPI------------------IARPT 180
            LRCVHN+PQGGHLPDYAPHPAIVLKMEDGTGLLLPI                  IARPT
Sbjct: 121 QLRCVHNNPQGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAMTNVQIARPT 180

Query: 181 IYQIMKEMIEKMGY----------------------AIDNEDECISFDLRPSDAINIAVR 240
           +YQ++KEM++KMGY                       + N  EC+SFDLRPSDAINIAVR
Sbjct: 181 MYQVVKEMVDKMGYEVRLVRVTKRVHEAYFAQLFLSKVGNASECVSFDLRPSDAINIAVR 240

Query: 241 CKVPIQVNRDLAHSDGMKIVESPKATIVGPASDVLSFTELDRPNGKPCIETTEFNLLQNM 271
           CK+PIQVN+ LA+SDGM+++ES K +   PASD L FTE DRPNG+ C++T EFN+L  M
Sbjct: 241 CKIPIQVNKYLAYSDGMRVIESGKISTPAPASDGLLFTEQDRPNGQACLDTKEFNILSKM 300
[Close]
BLAST of Spo20114.1 vs. ExPASy Swiss-Prot
Match: BBD2_ARATH (Bifunctional nuclease 2 OS=Arabidopsis thaliana GN=BBD2 PE=2 SV=1)

HSP 1 Score: 315.5 bits (807), Expect = 5.900e-85
Identity = 163/310 (52.58%), Postives = 210/310 (67.74%), Query Frame = 1 

		  

Query: 1   MGSLHGPVVCPAIHSKQASVYQIPINVSSMKDDMFRSTFWGYKGVRQRTTNAGILTRPIN 60
           M SL  PVVCP++  +Q  V  + +N S  K    R  FWG +    ++  A +  R ++
Sbjct: 1   MRSLQAPVVCPSVRPRQLGVSALLVNCSVSKTRSLRKQFWGNQTKNDKSQAATVNLR-LH 60

Query: 61  IRRFGTVQCTFSSSSNGSDSRAENFKENDEDYVNSSVTEAVEVKSGSGGFTIKMRDGRFL 120
           +RR+ +++C FSS S+G+ S AENF ENDEDYV SSV EAVEVKSG  GF +KM+DGR L
Sbjct: 61  LRRYKSIKCLFSSHSDGTGSTAENFNENDEDYVKSSVLEAVEVKSGPDGFMVKMKDGRQL 120

Query: 121 RCVHNSPQGGHLPDYAPHPAIVLKMEDGTGLLLPI------------------IARPTIY 180
           RCVHN+PQGG+LP+YAPH AIVLKMEDGTGLLLPI                  IARPT+Y
Sbjct: 121 RCVHNNPQGGNLPNYAPHSAIVLKMEDGTGLLLPIIVLEMPSVLLMAAMTNVQIARPTMY 180

Query: 181 QIMKEMIEKMGY----------------------AIDNEDECISFDLRPSDAINIAVRCK 240
           Q++K+M++KMGY                       + ++ +C+SFDLRPSDAINIAVRCK
Sbjct: 181 QVVKDMVDKMGYEVRLVRVTTRVHEAYFAELYLSKVGDKSDCVSFDLRPSDAINIAVRCK 240

Query: 241 VPIQVNRDLAHSDGMKIVESPKATIVGPASDVLSFTELDRPNGKPCIETTEFNLLQNMLN 271
           VPIQVN+ LA+SDGM++++S K +   PASD L FTELDRPNG+PC +T EF+L++NM+ 
Sbjct: 241 VPIQVNKYLAYSDGMRVIDSGKLSKQTPASDGLLFTELDRPNGQPCFDTKEFDLVRNMMQ 300
[Close]
BLAST of Spo20114.1 vs. ExPASy Swiss-Prot
Match: BBD2_ORYSJ (Bifunctional nuclease 2 OS=Oryza sativa subsp. japonica GN=BBD2 PE=2 SV=1)

HSP 1 Score: 253.8 bits (647), Expect = 2.100e-66
Identity = 147/311 (47.27%), Postives = 194/311 (62.38%), Query Frame = 1 

		  

Query: 3   SLHGPVVCPAIHSKQASVYQIPINVSSMKDDMFRSTFWGYK-GVRQRTTNAGILTRPINI 62
           ++ GP++C A+   QA +    I+ S  K     + F G     R  T     + +P   
Sbjct: 2   AMEGPILCRAV--MQAKLPVTMISNSLTKSGQLGTAFLGCVCKYRNITRLISPIYQPAQ- 61

Query: 63  RRFGTVQCTFSSSSNGSDSRAENFKENDEDYVNSSVTEAVEVKSGSGGFTIKMRDGRFLR 122
           + F TV  +FSSSS+G+   A NF E+DEDYVNS+V EAVEV+SG+ G+ IKMRDG+ LR
Sbjct: 62  KNFATVCGSFSSSSDGNGYMAGNFSESDEDYVNSTVLEAVEVRSGAEGYVIKMRDGKNLR 121

Query: 123 CVHNSPQGGHLPDYAPHPAIVLKMEDGTGLLLPII------------------ARPTIYQ 182
           CVHN+ QG ++P+ AP PAIVL++EDG+  LLPII                  ARPTIYQ
Sbjct: 122 CVHNNSQGRNIPESAPQPAIVLRIEDGSETLLPIIVLEMPSVLLMAAIRNVHIARPTIYQ 181

Query: 183 IMKEMIEKMGYAID----------------------NEDECISFDLRPSDAINIAVRCKV 242
           ++KEMI+KMGY +                       +  E I+FDLRPSDAINIAVRCKV
Sbjct: 182 VVKEMIDKMGYEVKLVRINKRIQEAYCAELFLTKVGDHTESITFDLRPSDAINIAVRCKV 241

Query: 243 PIQVNRDLAHSDGMKIVESPKATIVGPASDVLSFTELDRPNGKPCIETTEFNLLQNMLNA 273
           PIQV+R LA+SDG++ VE  +  I    SD L FTELDRP+G+PC+E  EF L++NML A
Sbjct: 242 PIQVHRSLAYSDGIRSVEPARMAIAAGMSDGLLFTELDRPDGQPCVEAQEFGLIRNMLIA 301
[Close]
BLAST of Spo20114.1 vs. ExPASy Swiss-Prot
Match: BBD_ORYMI (Bifunctional nuclease OS=Oryza minuta GN=BBD PE=2 SV=1)

HSP 1 Score: 216.5 bits (550), Expect = 3.700e-55
Identity = 121/245 (49.39%), Postives = 160/245 (65.31%), Query Frame = 1 

		  

Query: 67  VQCTFSSSSNGSDSRAENFKENDEDYVNSSVTEAVEVKSGSGGFTIKMRDGRFLRCVHNS 126
           V C++ SSS+G  + A ++  + E++VNSSV EAVE++S S GF IKMRDG+ LRCV N+
Sbjct: 71  VYCSYGSSSDGDGAAAADYDASGEEFVNSSVMEAVELRSVSDGFVIKMRDGKNLRCVQNN 130

Query: 127 PQGGHLPDYAPHPAIVLKMEDGTGLLLPIIA------------------RPTIYQIMKEM 186
           P+   L D APH AIVLKMEDG+ LLLPII                   RPTIY ++KEM
Sbjct: 131 PRVLRLRDSAPHHAIVLKMEDGSDLLLPIIVMETPSIMLLAALRNIRIPRPTIYNVVKEM 190

Query: 187 IEKMGYA----------------------IDNEDECISFDLRPSDAINIAVRCKVPIQVN 246
            E+MGYA                      I NE+E IS DL+PSDAINIA RCKVPIQVN
Sbjct: 191 TERMGYAVRLVRITEMVHDAYYSRLYLAKIGNEEETISLDLKPSDAINIAFRCKVPIQVN 250

Query: 247 RDLAHSDGMKIVE-SPKATIVGPASDVLSFTELDRPNGKPCIETTEFNLLQNMLNAALEE 271
           R +A+++G+K+V+ +P  + V  +SD   +T LDRP+ +PC E  EF+L++NML AA+EE
Sbjct: 251 RRIAYNNGLKVVQPTPSESYV--SSDQFQYTRLDRPDDQPCFEAQEFDLVRNMLVAAVEE 310
[Close]
BLAST of Spo20114.1 vs. ExPASy Swiss-Prot
Match: BBD1_ORYSJ (Bifunctional nuclease 1 OS=Oryza sativa subsp. japonica GN=BBD1 PE=2 SV=1)

HSP 1 Score: 215.7 bits (548), Expect = 6.400e-55
Identity = 121/245 (49.39%), Postives = 160/245 (65.31%), Query Frame = 1 

		  

Query: 67  VQCTFSSSSNGSDSRAENFKENDEDYVNSSVTEAVEVKSGSGGFTIKMRDGRFLRCVHNS 126
           V C++ SSS+G  + A ++  + E++VNSSV EAVE++S S GF IKMRDG+ LRCV N+
Sbjct: 71  VCCSYGSSSDGDGAAAADYDASGEEFVNSSVMEAVELRSVSDGFVIKMRDGKNLRCVQNN 130

Query: 127 PQGGHLPDYAPHPAIVLKMEDGTGLLLPIIA------------------RPTIYQIMKEM 186
           P+   L D APH AIVLKMEDG+ LLLPII                   RPTIY ++KEM
Sbjct: 131 PRVLRLRDSAPHHAIVLKMEDGSDLLLPIIVMETPSIMLLAALRNIRIPRPTIYNVVKEM 190

Query: 187 IEKMGYA----------------------IDNEDECISFDLRPSDAINIAVRCKVPIQVN 246
            E+MGYA                      I NE+E IS DL+PSDAINIA RCKVPIQVN
Sbjct: 191 TERMGYAVRLVRITEMVHDAYYSRLYLAKIGNEEETISLDLKPSDAINIAFRCKVPIQVN 250

Query: 247 RDLAHSDGMKIVE-SPKATIVGPASDVLSFTELDRPNGKPCIETTEFNLLQNMLNAALEE 271
           R +A+++G+K+V+ +P  + V  +SD   +T LDRP+ +PC E  EF+L++NML AA+EE
Sbjct: 251 RRIAYNNGLKVVQPTPSESYV--SSDQFQYTRLDRPDDQPCFEAQEFDLVRNMLVAAVEE 310
[Close]
BLAST of Spo20114.1 vs. TAIR (Arabidopsis)
Match: AT1G75380.1 (bifunctional nuclease in basal defense response 1)

HSP 1 Score: 320.1 bits (819), Expect = 1.400e-87
Identity = 171/312 (54.81%), Postives = 206/312 (66.03%), Query Frame = 1 

		  

Query: 1   MGSLHGPVVCPAIHSKQASVYQIPINVSSMKDDMFRSTFWGY--KGVRQRTTNAGILTRP 60
           M S+  PVVCPAI  +Q       +N + +K    RS FWG   KGV+ + T   I  R 
Sbjct: 1   MRSVQAPVVCPAIRPRQVGACASLVNYTGLKP---RSQFWGNRTKGVKSQGTTTTITLRL 60

Query: 61  INIRRFGTVQCTFSSSSNGSDSRAENFKENDEDYVNSSVTEAVEVKSGSGGFTIKMRDGR 120
            N     +++C FSS S+G+ S AENF ENDE+YVNSSV EAVEVKSG+ GF +KMRDGR
Sbjct: 61  CN----KSIKCVFSSHSDGNGSTAENFNENDEEYVNSSVVEAVEVKSGADGFMVKMRDGR 120

Query: 121 FLRCVHNSPQGGHLPDYAPHPAIVLKMEDGTGLLLPI------------------IARPT 180
            LRCVHN+PQGGHLPDYAPHPAIVLKMEDGTGLLLPI                  IARPT
Sbjct: 121 QLRCVHNNPQGGHLPDYAPHPAIVLKMEDGTGLLLPIIVLEMPSVLLMAAMTNVQIARPT 180

Query: 181 IYQIMKEMIEKMGY----------------------AIDNEDECISFDLRPSDAINIAVR 240
           +YQ++KEM++KMGY                       + N  EC+SFDLRPSDAINIAVR
Sbjct: 181 MYQVVKEMVDKMGYEVRLVRVTKRVHEAYFAQLFLSKVGNASECVSFDLRPSDAINIAVR 240

Query: 241 CKVPIQVNRDLAHSDGMKIVESPKATIVGPASDVLSFTELDRPNGKPCIETTEFNLLQNM 271
           CK+PIQVN+ LA+SDGM+++ES K +   PASD L FTE DRPNG+ C++T EFN+L  M
Sbjct: 241 CKIPIQVNKYLAYSDGMRVIESGKISTPAPASDGLLFTEQDRPNGQACLDTKEFNILSKM 300
[Close]
BLAST of Spo20114.1 vs. TAIR (Arabidopsis)
Match: AT1G19660.1 (Wound-responsive family protein)

HSP 1 Score: 315.5 bits (807), Expect = 3.300e-86
Identity = 163/310 (52.58%), Postives = 210/310 (67.74%), Query Frame = 1 

		  

Query: 1   MGSLHGPVVCPAIHSKQASVYQIPINVSSMKDDMFRSTFWGYKGVRQRTTNAGILTRPIN 60
           M SL  PVVCP++  +Q  V  + +N S  K    R  FWG +    ++  A +  R ++
Sbjct: 1   MRSLQAPVVCPSVRPRQLGVSALLVNCSVSKTRSLRKQFWGNQTKNDKSQAATVNLR-LH 60

Query: 61  IRRFGTVQCTFSSSSNGSDSRAENFKENDEDYVNSSVTEAVEVKSGSGGFTIKMRDGRFL 120
           +RR+ +++C FSS S+G+ S AENF ENDEDYV SSV EAVEVKSG  GF +KM+DGR L
Sbjct: 61  LRRYKSIKCLFSSHSDGTGSTAENFNENDEDYVKSSVLEAVEVKSGPDGFMVKMKDGRQL 120

Query: 121 RCVHNSPQGGHLPDYAPHPAIVLKMEDGTGLLLPI------------------IARPTIY 180
           RCVHN+PQGG+LP+YAPH AIVLKMEDGTGLLLPI                  IARPT+Y
Sbjct: 121 RCVHNNPQGGNLPNYAPHSAIVLKMEDGTGLLLPIIVLEMPSVLLMAAMTNVQIARPTMY 180

Query: 181 QIMKEMIEKMGY----------------------AIDNEDECISFDLRPSDAINIAVRCK 240
           Q++K+M++KMGY                       + ++ +C+SFDLRPSDAINIAVRCK
Sbjct: 181 QVVKDMVDKMGYEVRLVRVTTRVHEAYFAELYLSKVGDKSDCVSFDLRPSDAINIAVRCK 240

Query: 241 VPIQVNRDLAHSDGMKIVESPKATIVGPASDVLSFTELDRPNGKPCIETTEFNLLQNMLN 271
           VPIQVN+ LA+SDGM++++S K +   PASD L FTELDRPNG+PC +T EF+L++NM+ 
Sbjct: 241 VPIQVNKYLAYSDGMRVIDSGKLSKQTPASDGLLFTELDRPNGQPCFDTKEFDLVRNMMQ 300
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BLAST of Spo20114.1 vs. TAIR (Arabidopsis)
Match: AT5G66050.1 (Wound-responsive family protein)

HSP 1 Score: 57.0 bits (136), Expect = 2.100e-8
Identity = 47/172 (27.33%), Postives = 72/172 (41.86%), Query Frame = 1 

		  

Query: 139 PAIVLKMEDGTGLLLPIIAR-------------------PTIYQIMKEMIEKMGYAID-- 198
           P I LK+      LLP+I                     P  +Q +  +++K+GY +   
Sbjct: 144 PTIFLKISCDGDYLLPVIVGDAAVEKLLDVPLQGHTEECPDQFQFVSAVVDKLGYEVKMV 203

Query: 199 -------------------NEDECISFDLRPSDAINIAVRCKVPIQVNRDLAHSDGMKIV 258
                               + E I  D RPSDAIN+A  C+ PI VN+ +   + +KI 
Sbjct: 204 KLTGRIVNTYYASLCLGKPGDIEAICIDSRPSDAINVARACQAPIYVNKAIVLEEAIKIG 263

Query: 259 ESPKATIVGPASDVLSFTELDRPNGKPCIETTEFNLLQNMLNAALEERYQEA 271
              +     P  +V+  +  D P+  P  E  E  L++NM  A+ EERY +A
Sbjct: 264 YGGRPQSAKPVFNVILDSAPDGPD--PLSE--ELKLVRNMDLASKEERYIDA 311
The following BLAST results are available for this feature:
BLAST of Spo20114.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5
Match NameE-valueIdentityDescription
gi|902235563|gb|KNA23976.1|7.9e-14687.1hypothetical protein SOVF_0202... [more]
gi|731312202|ref|XP_010673061.1|2.5e-12373.8PREDICTED: bifunctional nuclea... [more]
gi|147842247|emb|CAN76215.1|7.2e-9959.4hypothetical protein VITISV_00... [more]
gi|641865955|gb|KDO84640.1|1.4e-9759.9hypothetical protein CISIN_1g0... [more]
gi|568839128|ref|XP_006473546.1|3.0e-9760.1PREDICTED: bifunctional nuclea... [more]
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BLAST of Spo20114.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0K9RWT8_SPIOL5.5e-14687.1Uncharacterized protein OS=Spi... [more]
A0A0J8D8Y6_BETVU1.7e-12373.8Uncharacterized protein OS=Bet... [more]
A5AZ38_VITVI5.0e-9959.4Putative uncharacterized prote... [more]
A0A067H158_CITSI9.5e-9859.9Uncharacterized protein OS=Cit... [more]
E0CQH3_VITVI3.6e-9758.7Putative uncharacterized prote... [more]
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BLAST of Spo20114.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5
Match NameE-valueIdentityDescription
BBD1_ARATH2.4e-8654.8Bifunctional nuclease 1 OS=Ara... [more]
BBD2_ARATH5.9e-8552.5Bifunctional nuclease 2 OS=Ara... [more]
BBD2_ORYSJ2.1e-6647.2Bifunctional nuclease 2 OS=Ory... [more]
BBD_ORYMI3.7e-5549.3Bifunctional nuclease OS=Oryza... [more]
BBD1_ORYSJ6.4e-5549.3Bifunctional nuclease 1 OS=Ory... [more]
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BLAST of Spo20114.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 3
Match NameE-valueIdentityDescription
AT1G75380.11.4e-8754.8bifunctional nuclease in basal... [more]
AT1G19660.13.3e-8652.5Wound-responsive family protei... [more]
AT5G66050.12.1e-827.3Wound-responsive family protei... [more]
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InterPro
Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003729Bifunctional nuclease domainPFAMPF02577DNase-RNasecoord: 180..215
score: 7.
IPR003729Bifunctional nuclease domainunknownSSF103256Hypothetical protein TM0160coord: 122..221
score: 3.4
NoneNo IPR availablePANTHERPTHR15160VON HIPPEL-LINDAU PROTEINcoord: 42..271
score: 1.7E
NoneNo IPR availablePANTHERPTHR15160:SF3BIFUNCTIONAL NUCLEASE 1-RELATEDcoord: 42..271
score: 1.7E

GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0004518 nuclease activity