Spo20876.1 (mRNA)

Overview
NameSpo20876.1
TypemRNA
OrganismSpinacia oleracea (Spinach)
DescriptionTranslocon-associated protein subunit beta (TRAP-beta) (Signal sequence receptor subunit beta)
LocationSpoScf_01987 : 81312 .. 83024 (-)
Sequence length755
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexonthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTTTTACGGTTTCGTGCTCTAAATTGGTAGGTTATGAATATTGGGTTCTAATTTTCCATCTTGAGAATGGGTGGCTAAATTACTTAGAATCATCGTTCATTGTAGTGCACAAGAAGGCTACCCTCACTAATCTCGAAAATGGCGTCAAAGGTATCTCTGTTTCTACCGATATTTACAACCATGGACTTCTGTATACTGTTCTTTTATCTCTCCTAATTTTGCACTTGCAATCACGATTTCAGATATCTTGTTTTCGTTTGATTTTCCATTGCTTTTAAGTTTTTAAATTGAATGCTTGCGTGCTTGATTTTGGTTGCATATTGATTTGGGAGTCAATTCAGTTGGAATTGAAATTTGATTACAATTTCATTGTTTAATCTTGAATTTTGTGCAGTTGATTAATTTTTTGAGTTTACAAATTGATCGACAATACTTGTATGTTAATCGAAAACTTAAACTGATTTTACATGAATTAGTTCCTATTTAAAGTACTAATGTATTGAAAAACTAGAATCATTTCCCTGGAAAAGCATATTGGAGATTAAAGTTCTCAAGCCTGTATGTCCAAATTTTTTTATGTTTAACTCAGTCCTGTAGTTTTGATAATGTGATTGTCATTGACAAAAAATCGCGTTTCTGTTAGACCTCAATAATGAAATAGCAGTAACGAGTATTAGTAATAACCAAATATCGCATTTCTGTTATGCCTCGTGATCAAAACAGTAGTAATAAGATAAGGATCGAATATCACATTTCAATTCTATTAGATCTCATGAATAAAATTTGAGTTATGACTTGTGAGTATAGGTAATGATCGAAAATCACATTTCAGAAACCCCATGAGGCCATGATGTTTCATAATTTTATTGATATTACCATAACTAGTCAAAGGGACTTGATGAGTATTGCAGCTGTTTTTGACATGAATGGATGAAGCCTTGACATATTTTAGAATTGACAAAATTTTATGTTTAGATTGGCTACTTTCATTAGTTCTTACACTATAGGGAATACGATGCATTGAGTTCCTGTTCATGTCAATTTTGTTATGTCTAACAAAATGATAACAATTGAGTTCCGCAATGGTGCTCTTTTTCGTCTGCCTATTAGATGTCTAACATGTGTTTATATTTGATTAATGCATTCAGAGCTGTGTATGATATAAGCCATGTCGACAAGAACTGGCCAACAGATTCATTTGATGTGGCCAGTGGCAACTTTTCAAGATCATGGGACGGCCTTGATGTGTGAGTATTTATTTACTAGCAATTAAACTATATTTTCTCTCCGGGAGTTCCTTAGTTGTGAATCTGTAAACTGATTGTTGTTATTCTCAATTGGGAGGAAGTCAAAGATCATAAAGCAGAGAGAGAAAGGGGTTAGAGAACAAATTTGAGTTCCATAATAACCACCATCAAATATCTAGAGCGCCATGATGTTGGGAAATCATTGTATATGTTGAAGAACCCTGTAATATTCATTTAATATTCTATTATGCTTGTGAAGAACTTAAGACCTTATCTCAGCGTATGCTTTGAGTTCATAAACTCGCTTCTCAACATCTTTCCCTGTGCTTCCATAAGTAACAATTGGAACAACATCTTTCCACGTGTCATTACTCCACAGCTGTCAGCTATATTGATATGTTTGATAGTTGAAACTGTAAATCTAAGTGGAGTTTTTCTTTCTTATATGACACTTATCTAGAC

mRNA sequence

ATGGGTTTTACGGTTTCGTGCTCTAAATTGGTAGGTTATGAATATTGGGTTCTAATTTTCCATCTTGAGAATGGGTGGCTAAATTACTTAGAATCATCGTTCATTGTAGTGCACAAGAAGGCTACCCTCACTAATCTCGAAAATGGCGTCAAAGGTATCTCTGTTTCTACCGATATTTACAACCATGGACTTCTAGCTGTGTATGATATAAGCCATGTCGACAAGAACTGGCCAACAGATTCATTTGATGTGGCCAGTGGCAACTTTTCAAGATCATGGGACGGCCTTGATGTGTGAGTATTTATTTACTAGCAATTAAACTATATTTTCTCTCCGGGAGTTCCTTAGTTGTGAATCTGTAAACTGATTGTTGTTATTCTCAATTGGGAGGAAGTCAAAGATCATAAAGCAGAGAGAGAAAGGGGTTAGAGAACAAATTTGAGTTCCATAATAACCACCATCAAATATCTAGAGCGCCATGATGTTGGGAAATCATTGTATATGTTGAAGAACCCTGTAATATTCATTTAATATTCTATTATGCTTGTGAAGAACTTAAGACCTTATCTCAGCGTATGCTTTGAGTTCATAAACTCGCTTCTCAACATCTTTCCCTGTGCTTCCATAAGTAACAATTGGAACAACATCTTTCCACGTGTCATTACTCCACAGCTGTCAGCTATATTGATATGTTTGATAGTTGAAACTGTAAATCTAAGTGGAGTTTTTCTTTCTTATATGACACTTATCTAGAC

Coding sequence (CDS)

ATGGGTTTTACGGTTTCGTGCTCTAAATTGGTAGGTTATGAATATTGGGTTCTAATTTTCCATCTTGAGAATGGGTGGCTAAATTACTTAGAATCATCGTTCATTGTAGTGCACAAGAAGGCTACCCTCACTAATCTCGAAAATGGCGTCAAAGGTATCTCTGTTTCTACCGATATTTACAACCATGGACTTCTAGCTGTGTATGATATAAGCCATGTCGACAAGAACTGGCCAACAGATTCATTTGATGTGGCCAGTGGCAACTTTTCAAGATCATGGGACGGCCTTGATGTGTGA

Protein sequence

MGFTVSCSKLVGYEYWVLIFHLENGWLNYLESSFIVVHKKATLTNLENGVKGISVSTDIYNHGLLAVYDISHVDKNWPTDSFDVASGNFSRSWDGLDV
Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Spo20876Spo20876gene


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Spo20876.1Spo20876.1-proteinpolypeptide


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
Spo20876.1.CDS.2Spo20876.1.CDS.2CDS
Spo20876.1.CDS.1Spo20876.1.CDS.1CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Spo20876.1.utr3p.1Spo20876.1.utr3p.1three_prime_UTR


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Spo20876.1.exon.2Spo20876.1.exon.2exon
Spo20876.1.exon.1Spo20876.1.exon.1exon


Homology
[Close]
BLAST of Spo20876.1 vs. NCBI nr
Match: gi|902236012|gb|KNA24175.1| (hypothetical protein SOVF_018150 [Spinacia oleracea])

HSP 1 Score: 112.5 bits (280), Expect = 4.300e-22
Identity = 53/67 (79.10%), Postives = 57/67 (85.07%), Query Frame = 1 

		  

Query: 31 ESSFIVVHKKATLTNLENGVKGISVSTDIYNHGLLAVYDISHVDKNWPTDSFDVASGNFS 90
          ES+FIV HKKATLT L+NGV+ ISVS DIYN G L VYD+S VDKNWPTDSFDV SGNFS
Sbjct: 27 ESAFIVAHKKATLTKLKNGVERISVSIDIYNRGSLTVYDVSLVDKNWPTDSFDVVSGNFS 86

Query: 91 RSWDGLD 98
          RSWD LD
Sbjct: 87 RSWDSLD 93
[Close]
BLAST of Spo20876.1 vs. NCBI nr
Match: gi|763803755|gb|KJB70693.1| (hypothetical protein B456_011G087400 [Gossypium raimondii])

HSP 1 Score: 86.7 bits (213), Expect = 2.500e-14
Identity = 38/67 (56.72%), Postives = 50/67 (74.63%), Query Frame = 1 

		  

Query: 31 ESSFIVVHKKATLTNLENGVKGISVSTDIYNHGLLAVYDISHVDKNWPTDSFDVASGNFS 90
          +S FI+ HKKA+LT L++G + +SVS DIYN G    YD+S VD +WP D+FDV SGN S
Sbjct: 27 DSPFIIAHKKASLTRLKSGAERVSVSIDIYNQGFSTAYDLSLVDYSWPQDAFDVISGNIS 86

Query: 91 RSWDGLD 98
          +SW+ LD
Sbjct: 87 QSWEKLD 93
[Close]
BLAST of Spo20876.1 vs. NCBI nr
Match: gi|763803754|gb|KJB70692.1| (hypothetical protein B456_011G087400 [Gossypium raimondii])

HSP 1 Score: 86.7 bits (213), Expect = 2.500e-14
Identity = 38/67 (56.72%), Postives = 50/67 (74.63%), Query Frame = 1 

		  

Query: 31 ESSFIVVHKKATLTNLENGVKGISVSTDIYNHGLLAVYDISHVDKNWPTDSFDVASGNFS 90
          +S FI+ HKKA+LT L++G + +SVS DIYN G    YD+S VD +WP D+FDV SGN S
Sbjct: 27 DSPFIIAHKKASLTRLKSGAERVSVSIDIYNQGFSTAYDLSLVDYSWPQDAFDVISGNIS 86

Query: 91 RSWDGLD 98
          +SW+ LD
Sbjct: 87 QSWEKLD 93
[Close]
BLAST of Spo20876.1 vs. NCBI nr
Match: gi|823248258|ref|XP_012456799.1| (PREDICTED: translocon-associated protein subunit beta [Gossypium raimondii])

HSP 1 Score: 86.7 bits (213), Expect = 2.500e-14
Identity = 38/67 (56.72%), Postives = 50/67 (74.63%), Query Frame = 1 

		  

Query: 31 ESSFIVVHKKATLTNLENGVKGISVSTDIYNHGLLAVYDISHVDKNWPTDSFDVASGNFS 90
          +S FI+ HKKA+LT L++G + +SVS DIYN G    YD+S VD +WP D+FDV SGN S
Sbjct: 27 DSPFIIAHKKASLTRLKSGAERVSVSIDIYNQGFSTAYDLSLVDYSWPQDAFDVISGNIS 86

Query: 91 RSWDGLD 98
          +SW+ LD
Sbjct: 87 QSWEKLD 93
[Close]
BLAST of Spo20876.1 vs. NCBI nr
Match: gi|763803756|gb|KJB70694.1| (hypothetical protein B456_011G087400 [Gossypium raimondii])

HSP 1 Score: 86.7 bits (213), Expect = 2.500e-14
Identity = 38/67 (56.72%), Postives = 50/67 (74.63%), Query Frame = 1 

		  

Query: 31 ESSFIVVHKKATLTNLENGVKGISVSTDIYNHGLLAVYDISHVDKNWPTDSFDVASGNFS 90
          +S FI+ HKKA+LT L++G + +SVS DIYN G    YD+S VD +WP D+FDV SGN S
Sbjct: 27 DSPFIIAHKKASLTRLKSGAERVSVSIDIYNQGFSTAYDLSLVDYSWPQDAFDVISGNIS 86

Query: 91 RSWDGLD 98
          +SW+ LD
Sbjct: 87 QSWEKLD 93
[Close]
BLAST of Spo20876.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RXC0_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_018150 PE=4 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 3.000e-22
Identity = 53/67 (79.10%), Postives = 57/67 (85.07%), Query Frame = 1 

		  

Query: 31 ESSFIVVHKKATLTNLENGVKGISVSTDIYNHGLLAVYDISHVDKNWPTDSFDVASGNFS 90
          ES+FIV HKKATLT L+NGV+ ISVS DIYN G L VYD+S VDKNWPTDSFDV SGNFS
Sbjct: 27 ESAFIVAHKKATLTKLKNGVERISVSIDIYNRGSLTVYDVSLVDKNWPTDSFDVVSGNFS 86

Query: 91 RSWDGLD 98
          RSWD LD
Sbjct: 87 RSWDSLD 93
[Close]
BLAST of Spo20876.1 vs. UniProtKB/TrEMBL
Match: A0A0D2RJ02_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_011G087400 PE=4 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 1.800e-14
Identity = 38/67 (56.72%), Postives = 50/67 (74.63%), Query Frame = 1 

		  

Query: 31 ESSFIVVHKKATLTNLENGVKGISVSTDIYNHGLLAVYDISHVDKNWPTDSFDVASGNFS 90
          +S FI+ HKKA+LT L++G + +SVS DIYN G    YD+S VD +WP D+FDV SGN S
Sbjct: 27 DSPFIIAHKKASLTRLKSGAERVSVSIDIYNQGFSTAYDLSLVDYSWPQDAFDVISGNIS 86

Query: 91 RSWDGLD 98
          +SW+ LD
Sbjct: 87 QSWEKLD 93
[Close]
BLAST of Spo20876.1 vs. UniProtKB/TrEMBL
Match: A0A0D2T4R0_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_011G087400 PE=4 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 1.800e-14
Identity = 38/67 (56.72%), Postives = 50/67 (74.63%), Query Frame = 1 

		  

Query: 31 ESSFIVVHKKATLTNLENGVKGISVSTDIYNHGLLAVYDISHVDKNWPTDSFDVASGNFS 90
          +S FI+ HKKA+LT L++G + +SVS DIYN G    YD+S VD +WP D+FDV SGN S
Sbjct: 27 DSPFIIAHKKASLTRLKSGAERVSVSIDIYNQGFSTAYDLSLVDYSWPQDAFDVISGNIS 86

Query: 91 RSWDGLD 98
          +SW+ LD
Sbjct: 87 QSWEKLD 93
[Close]
BLAST of Spo20876.1 vs. UniProtKB/TrEMBL
Match: A0A0D2UPY1_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_011G087400 PE=4 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 1.800e-14
Identity = 38/67 (56.72%), Postives = 50/67 (74.63%), Query Frame = 1 

		  

Query: 31 ESSFIVVHKKATLTNLENGVKGISVSTDIYNHGLLAVYDISHVDKNWPTDSFDVASGNFS 90
          +S FI+ HKKA+LT L++G + +SVS DIYN G    YD+S VD +WP D+FDV SGN S
Sbjct: 27 DSPFIIAHKKASLTRLKSGAERVSVSIDIYNQGFSTAYDLSLVDYSWPQDAFDVISGNIS 86

Query: 91 RSWDGLD 98
          +SW+ LD
Sbjct: 87 QSWEKLD 93
[Close]
BLAST of Spo20876.1 vs. UniProtKB/TrEMBL
Match: A0A0D2VK17_GOSRA (Uncharacterized protein OS=Gossypium raimondii GN=B456_011G087400 PE=4 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 1.800e-14
Identity = 38/67 (56.72%), Postives = 50/67 (74.63%), Query Frame = 1 

		  

Query: 31 ESSFIVVHKKATLTNLENGVKGISVSTDIYNHGLLAVYDISHVDKNWPTDSFDVASGNFS 90
          +S FI+ HKKA+LT L++G + +SVS DIYN G    YD+S VD +WP D+FDV SGN S
Sbjct: 27 DSPFIIAHKKASLTRLKSGAERVSVSIDIYNQGFSTAYDLSLVDYSWPQDAFDVISGNIS 86

Query: 91 RSWDGLD 98
          +SW+ LD
Sbjct: 87 QSWEKLD 93
[Close]
BLAST of Spo20876.1 vs. TAIR (Arabidopsis)
Match: AT5G14030.1 (translocon-associated protein beta (TRAPB) family protein)

HSP 1 Score: 75.1 bits (183), Expect = 2.700e-14
Identity = 35/79 (44.30%), Postives = 51/79 (64.56%), Query Frame = 1 

		  

Query: 19 IFHLENGWLNYLESSFIVVHKKATLTNLENGVKGISVSTDIYNHGLLAVYDISHVDKNWP 78
          +F L +      E  F+VVHKKATL  L++G + +SVS DIYN G  + YD++  D +W 
Sbjct: 15 VFMLVSASFATSEVPFMVVHKKATLNRLKSGAERVSVSYDIYNQGSSSAYDVTLTDNSWD 74

Query: 79 TDSFDVASGNFSRSWDGLD 98
            +F+V +GN S+SW+ LD
Sbjct: 75 KKTFEVVNGNTSKSWERLD 93
The following BLAST results are available for this feature:
BLAST of Spo20876.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5
Match NameE-valueIdentityDescription
gi|902236012|gb|KNA24175.1|4.3e-2279.1hypothetical protein SOVF_0181... [more]
gi|763803755|gb|KJB70693.1|2.5e-1456.7hypothetical protein B456_011G... [more]
gi|763803754|gb|KJB70692.1|2.5e-1456.7hypothetical protein B456_011G... [more]
gi|823248258|ref|XP_012456799.1|2.5e-1456.7PREDICTED: translocon-associat... [more]
gi|763803756|gb|KJB70694.1|2.5e-1456.7hypothetical protein B456_011G... [more]
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BLAST of Spo20876.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0K9RXC0_SPIOL3.0e-2279.1Uncharacterized protein OS=Spi... [more]
A0A0D2RJ02_GOSRA1.8e-1456.7Uncharacterized protein OS=Gos... [more]
A0A0D2T4R0_GOSRA1.8e-1456.7Uncharacterized protein OS=Gos... [more]
A0A0D2UPY1_GOSRA1.8e-1456.7Uncharacterized protein OS=Gos... [more]
A0A0D2VK17_GOSRA1.8e-1456.7Uncharacterized protein OS=Gos... [more]
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BLAST of Spo20876.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Spo20876.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 1
Match NameE-valueIdentityDescription
AT5G14030.12.7e-1444.3translocon-associated protein ... [more]
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InterPro
Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008856Translocon-associated protein subunit betaPANTHERPTHR12861TRANSLOCON-ASSOCIATED PROTEIN, BETA SUBUNIT PRECURSOR TRAP-BETA SIGNAL SEQUENCE RECEPTOR BETA SUBUNITcoord: 8..98
score: 2.5
NoneNo IPR availablePFAMPF05753TRAP_betacoord: 27..97
score: 1.2

GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0098588 bounding membrane of organelle
cellular_component GO:0044446 intracellular organelle part