Spo21589.1 (mRNA) | SpinachBase

Overview


Name	Spo21589.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	Isocitrate lyase (ICL) (Isocitrase) (Isocitratase) (4.1.3.1)
Location	chr2 : 55797907 .. 55801099 (+)
Sequence length	1589

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ACTAAAGAGTAATGTTTACACTTTACAGTAGCCACCTTCTAATATTTTCCTCTAACCAAATTTAACCAAAAAACAAACCACGAAATTTCCTAACGTTAAAAAAAAAAAAAAAACAGCGCACGTTATTTCGCACCAATTTGATCAAATATTATATATTTTGTTGCAACTTCAGTTTTTAAAATATGATGACTACTGTCTACTACTAGTGTGTACATACTATTCTTGGTAGTGTAGTACTATAAATAGAAGAGAAAACAACACAAACATAATCACAACACACACAACTTCTATCAAGTGATCATCCTCTTCTTCCTCCTTCCTTTTACAAAATAATATTTTCTCTATTTCTTAGCTTTATTACTTTTTACTAAAAATGGGTTCTCTTGAGGCTGCCGCTCCTCTAGGGAGGACAACCGTGCACCGCTTGATCGCGGAGGAAGGATCGGTGTTGATGCCCGGTATCCAAGACGCCTTCTCCGCCGCCATTTGTTACAAAACTGGCTTCAAAGCTTGTTTTGTCTCTGGCTTTGGTGTCTCTGCCGCCCTCCTTGGCTTGCCTGACTTTGGCTTGCTCACGTAAGATATCTCTCTCATTTCTCCATGTTATTGTAATTCAATATTTTTATTATTGATAGATATATTGCATTGGTATATAAAACTGACATATTATACGAGTTTGATCATATTTTAAGTCGATCAGCTCCGTTTTAAAATGATCTTTACGCTTTCCGTTTTAGTCTGTTTCGTAATGTTCTTTATTCTTTGCTTTATTATATTTTTGGACACAAAAATTTACTACTTTACTCGTTTTATTCCACAATATTTACAATTTTCCACTCACTTTCTCTTACTTTATTCATTTTTTTCTTACAGAAACCCACATTTTTACATATTTCTCTTACTTTATCCATATATTGTTATATTCAACCAACTTTTCTACTTTTATTTTAATACTTGTGCAAATAATAACTGTAAAAATAATTATGAAACGGAGATATATAGTATTACATTACCCTACATTGATATTTAAAATGACATTTGCATTGATATTATAGGACGATAAGATCGATATGAACAATTTAAGTGTGACGGGTGCATGTAAAATCATGTGTTTGTATAGTTACATTATATACAGTCAACATAATGGGACCCAATCTGTAACAATTTATGTTTTCTGAAGTAAAGCCACCTATATGTCACGGACGGGCCTTTTGACCGCCCGCGCGGCGCCTGAATCCCCGAGGCTTGATCCAGGCCAGCCTACCCCATATGCGCAACAAGCATTGTACAACACAATAGTAGAGGAAACAACACGTAATGATACTAACCTGTACCAAGATACATGCATCAGTAAGGCATACAGGCTCATGGAAGTTGCCTCATATGGGACACGCTGGGCCTTAACATCGAGAAAATATCAACACGTGACATATAGCTACTAATTAATCAATGTTGCGTACCATTAATAGTAGTGGCCCCATTTCTCCATGTTAACTCAATATTTGTTGTTTAAGATGAAATTTCAAAATTTTACCCTACAAAAATATCAATGGATATTTATAAATGACTTTTGGTGTAAATTACCGAAATCCATTGAATTTTGGATTTAAAAAAATGACGTGTTACTAACCTTTGACCCGTGCGGCCATGCCAACATTAAAATATTTTTTTAGACATAATTTATTGTCAATGAAAGAGTCTTAGTTAATAATTCATAGTCCATATCTTTATAAATATTATAATAATGGAAATCTTAATTTGTGATACAGAACAACAGAAGTAGTAGGAACTGTTCGTAGAATTACCGCTGCTGCTCCAAACTTGTGTGTCGTTGTCGATGGAGGTAACAAATATAACTTGCTAATCTTCATATTTATTCAACGGTTTAAAAGTTAAAGATGAATTATAATTAATTGAGTAAAAACAGACACTGGAGGGGGTGGCCCACTTAATGTTCAAAGGTTCATCAAAGAGTTGATTGCTGCTGGTGCCAAGGGTGTCTTTCTTGAGGTAAACTTTTGAATCAAAATTAAATGAAAAGTAAATTCAATTAGTATAATACTTATTATTTATTAATCAAATTAATGAATTTGATTTTGTTGTTCATATATAGGATCAAGTATGGCCCAAAAAGTGTGGTAAGTACAAAGTACTATTAATTAATTAACATCATCATTATTTTGAAAACATGTTAATTTAGTAATTCATATTTTTAAAAAAATTATTTACTCCTTGTTTGGTTAAATTTTTAGGTCATATGCGTGGTAAGAGTGTTGTACCGGCAGAGGAGCATGCACTTAAAATAGCAGCTGCAAGAGAAGCTATTGGTGATTCTGATTTCTTCTTGGTAGCAAGGTCTGATGCAAGGGCTCCCCATGGCCTTCAAGAAGCTATTCGACGGGCTAACCTTTACAGAGAGGTATCATATGAATATGAGAACTTGAGAAATGATGAATTATTAATGGTGTTATCTAATTATTTGTGTTAGGAGTACATTTTATTGATAGTTTTATTTAATTTGTTTTGTAGGCTGGAGCTGACGCAACCTTTGTCGAGGCACCTGCAAATCTTGAAGAGCTAAAAGAGGTTGTTAGGGATGTTAAGGGTTTGAGAATTGCTAACATGATTGAAGGTGGAAAGACTCCATTACACACACCCGCAGAGTTTAAGGAAATGGGATTCCACTTGATTGCTCATTCACTCTCAACAATTTATGCCACAACCAAAGCATTGGTTAGCATTATGAAGGTTCTTAAGGATAAGGGTACTACCAGGGATGACTTGGATCAGATTGTTACTTTCTCTGAGTTTAATGATATGATCAGCTTGGAGTCTTGGTATGAGCTTGAATCAAAGTTCAAGACCTTTACTCCAAAATCTTTGGAATCATAAGATGTGCGTGTTTATCTAGCATGCCAATTTGAGGCGATAGAAGTTAAGCGTTTAAAATAATTTCAAATCGATCATATTATTTTTCTTGTTTAGAGTTGTTTGAAATAAGGCATGGGTAATGCTTGTTAGTTTGATTTGTAGTGAAAGATCAAGATTCATATGATATTATGATGTATTCTTTTGTTTTGTTTAGCATTCCATGAGTTTATTTATGTTACGTTACTTTCCATGTAAGAAAATGAATAAAATAATTTATTCTTGCTTATTTGCTTCTAGATTTCTATATAATTTTGTTGTGGGG

mRNA sequence

ACTAAAGAGTAATGTTTACACTTTACAGTAGCCACCTTCTAATATTTTCCTCTAACCAAATTTAACCAAAAAACAAACCACGAAATTTCCTAACGTTAAAAAAAAAAAAAAAACAGCGCACGTTATTTCGCACCAATTTGATCAAATATTATATATTTTGTTGCAACTTCAGTTTTTAAAATATGATGACTACTGTCTACTACTAGTGTGTACATACTATTCTTGGTAGTGTAGTACTATAAATAGAAGAGAAAACAACACAAACATAATCACAACACACACAACTTCTATCAAGTGATCATCCTCTTCTTCCTCCTTCCTTTTACAAAATAATATTTTCTCTATTTCTTAGCTTTATTACTTTTTACTAAAAATGGGTTCTCTTGAGGCTGCCGCTCCTCTAGGGAGGACAACCGTGCACCGCTTGATCGCGGAGGAAGGATCGGTGTTGATGCCCGGTATCCAAGACGCCTTCTCCGCCGCCATTTGTTACAAAACTGGCTTCAAAGCTTGTTTTGTCTCTGGCTTTGGTGTCTCTGCCGCCCTCCTTGGCTTGCCTGACTTTGGCTTGCTCACAACAACAGAAGTAGTAGGAACTGTTCGTAGAATTACCGCTGCTGCTCCAAACTTGTGTGTCGTTGTCGATGGAGACACTGGAGGGGGTGGCCCACTTAATGTTCAAAGGTTCATCAAAGAGTTGATTGCTGCTGGTGCCAAGGGTGTCTTTCTTGAGGATCAAGTATGGCCCAAAAAGTGTGGTCATATGCGTGGTAAGAGTGTTGTACCGGCAGAGGAGCATGCACTTAAAATAGCAGCTGCAAGAGAAGCTATTGGTGATTCTGATTTCTTCTTGGTAGCAAGGTCTGATGCAAGGGCTCCCCATGGCCTTCAAGAAGCTATTCGACGGGCTAACCTTTACAGAGAGGCTGGAGCTGACGCAACCTTTGTCGAGGCACCTGCAAATCTTGAAGAGCTAAAAGAGGTTGTTAGGGATGTTAAGGGTTTGAGAATTGCTAACATGATTGAAGGTGGAAAGACTCCATTACACACACCCGCAGAGTTTAAGGAAATGGGATTCCACTTGATTGCTCATTCACTCTCAACAATTTATGCCACAACCAAAGCATTGGTTAGCATTATGAAGGTTCTTAAGGATAAGGGTACTACCAGGGATGACTTGGATCAGATTGTTACTTTCTCTGAGTTTAATGATATGATCAGCTTGGAGTCTTGGTATGAGCTTGAATCAAAGTTCAAGACCTTTACTCCAAAATCTTTGGAATCATAAGATGTGCGTGTTTATCTAGCATGCCAATTTGAGGCGATAGAAGTTAAGCGTTTAAAATAATTTCAAATCGATCATATTATTTTTCTTGTTTAGAGTTGTTTGAAATAAGGCATGGGTAATGCTTGTTAGTTTGATTTGTAGTGAAAGATCAAGATTCATATGATATTATGATGTATTCTTTTGTTTTGTTTAGCATTCCATGAGTTTATTTATGTTACGTTACTTTCCATGTAAGAAAATGAATAAAATAATTTATTCTTGCTTATTTGCTTCTAGATTTCTATATAATTTTGTTGTGGGG

Coding sequence (CDS)

ATGGGTTCTCTTGAGGCTGCCGCTCCTCTAGGGAGGACAACCGTGCACCGCTTGATCGCGGAGGAAGGATCGGTGTTGATGCCCGGTATCCAAGACGCCTTCTCCGCCGCCATTTGTTACAAAACTGGCTTCAAAGCTTGTTTTGTCTCTGGCTTTGGTGTCTCTGCCGCCCTCCTTGGCTTGCCTGACTTTGGCTTGCTCACAACAACAGAAGTAGTAGGAACTGTTCGTAGAATTACCGCTGCTGCTCCAAACTTGTGTGTCGTTGTCGATGGAGACACTGGAGGGGGTGGCCCACTTAATGTTCAAAGGTTCATCAAAGAGTTGATTGCTGCTGGTGCCAAGGGTGTCTTTCTTGAGGATCAAGTATGGCCCAAAAAGTGTGGTCATATGCGTGGTAAGAGTGTTGTACCGGCAGAGGAGCATGCACTTAAAATAGCAGCTGCAAGAGAAGCTATTGGTGATTCTGATTTCTTCTTGGTAGCAAGGTCTGATGCAAGGGCTCCCCATGGCCTTCAAGAAGCTATTCGACGGGCTAACCTTTACAGAGAGGCTGGAGCTGACGCAACCTTTGTCGAGGCACCTGCAAATCTTGAAGAGCTAAAAGAGGTTGTTAGGGATGTTAAGGGTTTGAGAATTGCTAACATGATTGAAGGTGGAAAGACTCCATTACACACACCCGCAGAGTTTAAGGAAATGGGATTCCACTTGATTGCTCATTCACTCTCAACAATTTATGCCACAACCAAAGCATTGGTTAGCATTATGAAGGTTCTTAAGGATAAGGGTACTACCAGGGATGACTTGGATCAGATTGTTACTTTCTCTGAGTTTAATGATATGATCAGCTTGGAGTCTTGGTATGAGCTTGAATCAAAGTTCAAGACCTTTACTCCAAAATCTTTGGAATCATAA

Protein sequence

MGSLEAAAPLGRTTVHRLIAEEGSVLMPGIQDAFSAAICYKTGFKACFVSGFGVSAALLGLPDFGLLTTTEVVGTVRRITAAAPNLCVVVDGDTGGGGPLNVQRFIKELIAAGAKGVFLEDQVWPKKCGHMRGKSVVPAEEHALKIAAAREAIGDSDFFLVARSDARAPHGLQEAIRRANLYREAGADATFVEAPANLEELKEVVRDVKGLRIANMIEGGKTPLHTPAEFKEMGFHLIAHSLSTIYATTKALVSIMKVLKDKGTTRDDLDQIVTFSEFNDMISLESWYELESKFKTFTPKSLES

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo21589	Spo21589	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo21589.1	Spo21589.1-protein	polypeptide

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo21589.1.exon.1	Spo21589.1.exon.1	exon
Spo21589.1.exon.2	Spo21589.1.exon.2	exon
Spo21589.1.exon.3	Spo21589.1.exon.3	exon
Spo21589.1.exon.4	Spo21589.1.exon.4	exon
Spo21589.1.exon.5	Spo21589.1.exon.5	exon
Spo21589.1.exon.6	Spo21589.1.exon.6	exon

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo21589.1.utr5p.1	Spo21589.1.utr5p.1	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo21589.1.CDS.1	Spo21589.1.CDS.1	CDS
Spo21589.1.CDS.2	Spo21589.1.CDS.2	CDS
Spo21589.1.CDS.3	Spo21589.1.CDS.3	CDS
Spo21589.1.CDS.4	Spo21589.1.CDS.4	CDS
Spo21589.1.CDS.5	Spo21589.1.CDS.5	CDS
Spo21589.1.CDS.6	Spo21589.1.CDS.6	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo21589.1.utr3p.1	Spo21589.1.utr3p.1	three_prime_UTR

Homology

[Close]

BLAST of Spo21589.1 vs. NCBI nr
Match: gi|902167267|gb|KNA07347.1| (hypothetical protein SOVF_172690 [Spinacia oleracea])

HSP 1 Score: 595.9 bits (1535), Expect = 4.000e-167
Identity = 304/304 (100.00%), Postives = 304/304 (100.00%), Query Frame = 1


		  
Query: 1   MGSLEAAAPLGRTTVHRLIAEEGSVLMPGIQDAFSAAICYKTGFKACFVSGFGVSAALLG 60
           MGSLEAAAPLGRTTVHRLIAEEGSVLMPGIQDAFSAAICYKTGFKACFVSGFGVSAALLG
Sbjct: 1   MGSLEAAAPLGRTTVHRLIAEEGSVLMPGIQDAFSAAICYKTGFKACFVSGFGVSAALLG 60

Query: 61  LPDFGLLTTTEVVGTVRRITAAAPNLCVVVDGDTGGGGPLNVQRFIKELIAAGAKGVFLE 120
           LPDFGLLTTTEVVGTVRRITAAAPNLCVVVDGDTGGGGPLNVQRFIKELIAAGAKGVFLE
Sbjct: 61  LPDFGLLTTTEVVGTVRRITAAAPNLCVVVDGDTGGGGPLNVQRFIKELIAAGAKGVFLE 120

Query: 121 DQVWPKKCGHMRGKSVVPAEEHALKIAAAREAIGDSDFFLVARSDARAPHGLQEAIRRAN 180
           DQVWPKKCGHMRGKSVVPAEEHALKIAAAREAIGDSDFFLVARSDARAPHGLQEAIRRAN
Sbjct: 121 DQVWPKKCGHMRGKSVVPAEEHALKIAAAREAIGDSDFFLVARSDARAPHGLQEAIRRAN 180

Query: 181 LYREAGADATFVEAPANLEELKEVVRDVKGLRIANMIEGGKTPLHTPAEFKEMGFHLIAH 240
           LYREAGADATFVEAPANLEELKEVVRDVKGLRIANMIEGGKTPLHTPAEFKEMGFHLIAH
Sbjct: 181 LYREAGADATFVEAPANLEELKEVVRDVKGLRIANMIEGGKTPLHTPAEFKEMGFHLIAH 240

Query: 241 SLSTIYATTKALVSIMKVLKDKGTTRDDLDQIVTFSEFNDMISLESWYELESKFKTFTPK 300
           SLSTIYATTKALVSIMKVLKDKGTTRDDLDQIVTFSEFNDMISLESWYELESKFKTFTPK
Sbjct: 241 SLSTIYATTKALVSIMKVLKDKGTTRDDLDQIVTFSEFNDMISLESWYELESKFKTFTPK 300

Query: 301 SLES 305
           SLES
Sbjct: 301 SLES 304

[Close]

BLAST of Spo21589.1 vs. NCBI nr
Match: gi|902167268|gb|KNA07348.1| (hypothetical protein SOVF_172700 [Spinacia oleracea])

HSP 1 Score: 576.2 bits (1484), Expect = 3.300e-161
Identity = 294/304 (96.71%), Postives = 298/304 (98.03%), Query Frame = 1


		  
Query: 1   MGSLEAAAPLGRTTVHRLIAEEGSVLMPGIQDAFSAAICYKTGFKACFVSGFGVSAALLG 60
           MGSLEA+APLGRTTVHRLIAEEGSVLMPGIQDAFSAAIC KTGFKACFVSGFGVSAALLG
Sbjct: 1   MGSLEASAPLGRTTVHRLIAEEGSVLMPGIQDAFSAAICAKTGFKACFVSGFGVSAALLG 60

Query: 61  LPDFGLLTTTEVVGTVRRITAAAPNLCVVVDGDTGGGGPLNVQRFIKELIAAGAKGVFLE 120
           LPDFGLLTTTEVV TVRRITAAAP+LCVVVDGDTGGGGPLNVQRFIKELIAAGAKGVFLE
Sbjct: 61  LPDFGLLTTTEVVETVRRITAAAPDLCVVVDGDTGGGGPLNVQRFIKELIAAGAKGVFLE 120

Query: 121 DQVWPKKCGHMRGKSVVPAEEHALKIAAAREAIGDSDFFLVARSDARAPHGLQEAIRRAN 180
           DQVWPKKCGHMRGKSVVPAEEHALKIAAAREAIGDSDFFLVARSDARAPHGLQEAIRRAN
Sbjct: 121 DQVWPKKCGHMRGKSVVPAEEHALKIAAAREAIGDSDFFLVARSDARAPHGLQEAIRRAN 180

Query: 181 LYREAGADATFVEAPANLEELKEVVRDVKGLRIANMIEGGKTPLHTPAEFKEMGFHLIAH 240
           LYREAGADATFVEAPANLEELKEVVRDVKGLRIANMIEGGKTPLHTP EFKEMGFHLIAH
Sbjct: 181 LYREAGADATFVEAPANLEELKEVVRDVKGLRIANMIEGGKTPLHTPTEFKEMGFHLIAH 240

Query: 241 SLSTIYATTKALVSIMKVLKDKGTTRDDLDQIVTFSEFNDMISLESWYELESKFKTFTPK 300
           SLSTIYATTKALV IMKVLKDKGTTRDDL++IVTFSEFNDMISLESWYE ESKFK FTPK
Sbjct: 241 SLSTIYATTKALVDIMKVLKDKGTTRDDLEKIVTFSEFNDMISLESWYETESKFKNFTPK 300

Query: 301 SLES 305
           SLES
Sbjct: 301 SLES 304

[Close]

BLAST of Spo21589.1 vs. NCBI nr
Match: gi|902218930|gb|KNA18181.1| (hypothetical protein SOVF_073210 [Spinacia oleracea])

HSP 1 Score: 548.1 bits (1411), Expect = 9.500e-153
Identity = 278/306 (90.85%), Postives = 289/306 (94.44%), Query Frame = 1


		  
Query: 1   MGSLEAA--APLGRTTVHRLIAEEGSVLMPGIQDAFSAAICYKTGFKACFVSGFGVSAAL 60
           MGSL       +GRTTVHRLIAEEGSVLMPGIQD FSAAICYKTGFKACFVSGFGVSAAL
Sbjct: 1   MGSLAETNGVAVGRTTVHRLIAEEGSVLMPGIQDPFSAAICYKTGFKACFVSGFGVSAAL 60

Query: 61  LGLPDFGLLTTTEVVGTVRRITAAAPNLCVVVDGDTGGGGPLNVQRFIKELIAAGAKGVF 120
           LGLPDFGLLTT EVVG VRRITAA+PNLCVVVDGDTGGGGPLNVQRFIK+LIAAGAKGVF
Sbjct: 61  LGLPDFGLLTTAEVVGVVRRITAASPNLCVVVDGDTGGGGPLNVQRFIKDLIAAGAKGVF 120

Query: 121 LEDQVWPKKCGHMRGKSVVPAEEHALKIAAAREAIGDSDFFLVARSDARAPHGLQEAIRR 180
           LEDQVWPKKCGHMRGK+VVP EEHALKIAAAREAIGDSDFFLVAR+DARAPHGLQEAIRR
Sbjct: 121 LEDQVWPKKCGHMRGKAVVPVEEHALKIAAAREAIGDSDFFLVARTDARAPHGLQEAIRR 180

Query: 181 ANLYREAGADATFVEAPANLEELKEVVRDVKGLRIANMIEGGKTPLHTPAEFKEMGFHLI 240
           AN+YREAGADATFVEAPAN EEL EVV+ VKGLRIANMIEGGKTPLHTP EFKEMGFHLI
Sbjct: 181 ANIYREAGADATFVEAPANREELDEVVKGVKGLRIANMIEGGKTPLHTPGEFKEMGFHLI 240

Query: 241 AHSLSTIYATTKALVSIMKVLKDKGTTRDDLDQIVTFSEFNDMISLESWYELESKFKTFT 300
           AHSLST+YATTKALV IMKVLKDKGTTRDDLD++V+FSEFNDMISLESWYELESKFK FT
Sbjct: 241 AHSLSTLYATTKALVDIMKVLKDKGTTRDDLDKMVSFSEFNDMISLESWYELESKFKNFT 300

Query: 301 PKSLES 305
           PKSLES
Sbjct: 301 PKSLES 306

[Close]

BLAST of Spo21589.1 vs. NCBI nr
Match: gi|902218929|gb|KNA18180.1| (hypothetical protein SOVF_073200 [Spinacia oleracea])

HSP 1 Score: 546.2 bits (1406), Expect = 3.600e-152
Identity = 277/305 (90.82%), Postives = 288/305 (94.43%), Query Frame = 1


		  
Query: 1   MGSL-EAAAPLGRTTVHRLIAEEGSVLMPGIQDAFSAAICYKTGFKACFVSGFGVSAALL 60
           MGSL E  A +G+TTVHRLIAEEGSVLMPGIQDAFSAAICYKTGFKACFVSGFGVSAALL
Sbjct: 1   MGSLSEGNAAMGKTTVHRLIAEEGSVLMPGIQDAFSAAICYKTGFKACFVSGFGVSAALL 60

Query: 61  GLPDFGLLTTTEVVGTVRRITAAAPNLCVVVDGDTGGGGPLNVQRFIKELIAAGAKGVFL 120
           GLPDFGLLTT EVV  VRRITA +PNLCVVVDGDTGGGGPLNVQRFIK+LI AGAKGVFL
Sbjct: 61  GLPDFGLLTTAEVVEVVRRITAGSPNLCVVVDGDTGGGGPLNVQRFIKDLINAGAKGVFL 120

Query: 121 EDQVWPKKCGHMRGKSVVPAEEHALKIAAAREAIGDSDFFLVARSDARAPHGLQEAIRRA 180
           EDQVWPKKCGHMRGK+VVP EEHALKIAAAREAIGDSDFFLVAR+DARAPHGLQEAIRRA
Sbjct: 121 EDQVWPKKCGHMRGKAVVPVEEHALKIAAAREAIGDSDFFLVARTDARAPHGLQEAIRRA 180

Query: 181 NLYREAGADATFVEAPANLEELKEVVRDVKGLRIANMIEGGKTPLHTPAEFKEMGFHLIA 240
           N+YREAGADATFVEAPAN EEL EVV+ VKGLRIANMIEGGKTPLHTP EFKEMGFHLIA
Sbjct: 181 NIYREAGADATFVEAPANREELDEVVKGVKGLRIANMIEGGKTPLHTPGEFKEMGFHLIA 240

Query: 241 HSLSTIYATTKALVSIMKVLKDKGTTRDDLDQIVTFSEFNDMISLESWYELESKFKTFTP 300
           HSLST+YATTKALV IMKVLKDKGTTRDD+D+IVTFSEFNDMISLESWYE ESKFK FTP
Sbjct: 241 HSLSTLYATTKALVDIMKVLKDKGTTRDDMDKIVTFSEFNDMISLESWYETESKFKNFTP 300

Query: 301 KSLES 305
           KSLES
Sbjct: 301 KSLES 305

[Close]

BLAST of Spo21589.1 vs. NCBI nr
Match: gi|731362409|ref|XP_010692873.1| (PREDICTED: petal death protein-like [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 538.9 bits (1387), Expect = 5.800e-150
Identity = 272/302 (90.07%), Postives = 290/302 (96.03%), Query Frame = 1


		  
Query: 1   MGSLEAA-APLGRTTVHRLIAEEGSVLMPGIQDAFSAAICYKTGFKACFVSGFGVSAALL 60
           MGSL+A+ A  GRTTVHRLIAEEG++LMPGIQDAFSAAIC KTG KACFVSGFGVSAALL
Sbjct: 1   MGSLDASNAVEGRTTVHRLIAEEGALLMPGIQDAFSAAICAKTGHKACFVSGFGVSAALL 60

Query: 61  GLPDFGLLTTTEVVGTVRRITAAAPNLCVVVDGDTGGGGPLNVQRFIKELIAAGAKGVFL 120
           GLPDFGLLTT EVV  VRRITAAAP+LCVVVDGDTGGGGPLNVQRFIK+LIAAGAKGVFL
Sbjct: 61  GLPDFGLLTTAEVVDVVRRITAAAPSLCVVVDGDTGGGGPLNVQRFIKDLIAAGAKGVFL 120

Query: 121 EDQVWPKKCGHMRGKSVVPAEEHALKIAAAREAIGDSDFFLVARSDARAPHGLQEAIRRA 180
           EDQVWPKKCGHMRGK VVPAEEHALKIAAAR+AIGDSDFFLVARSDARAPHGLQEAIRRA
Sbjct: 121 EDQVWPKKCGHMRGKMVVPAEEHALKIAAARDAIGDSDFFLVARSDARAPHGLQEAIRRA 180

Query: 181 NLYREAGADATFVEAPANLEELKEVVRDVKGLRIANMIEGGKTPLHTPAEFKEMGFHLIA 240
           NLYRE+GADATFVEAPANLEELKEVVRDVKGLRIANMIEGGKTPLHTPAEFKEMGFHLIA
Sbjct: 181 NLYRESGADATFVEAPANLEELKEVVRDVKGLRIANMIEGGKTPLHTPAEFKEMGFHLIA 240

Query: 241 HSLSTIYATTKALVSIMKVLKDKGTTRDDLDQIVTFSEFNDMISLESWYELESKFKTFTP 300
           HSLST+YATTKAL+ I++ LK+KGTTRDD DQ+VTF+EFNDMISLESWYELE+KFK+FTP
Sbjct: 241 HSLSTLYATTKALMGILRTLKEKGTTRDDFDQMVTFNEFNDMISLESWYELETKFKSFTP 300

Query: 301 KS 302
           K+
Sbjct: 301 KA 302

[Close]

BLAST of Spo21589.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QJB6_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_172690 PE=4 SV=1)

HSP 1 Score: 595.9 bits (1535), Expect = 2.800e-167
Identity = 304/304 (100.00%), Postives = 304/304 (100.00%), Query Frame = 1


		  
Query: 1   MGSLEAAAPLGRTTVHRLIAEEGSVLMPGIQDAFSAAICYKTGFKACFVSGFGVSAALLG 60
           MGSLEAAAPLGRTTVHRLIAEEGSVLMPGIQDAFSAAICYKTGFKACFVSGFGVSAALLG
Sbjct: 1   MGSLEAAAPLGRTTVHRLIAEEGSVLMPGIQDAFSAAICYKTGFKACFVSGFGVSAALLG 60

Query: 61  LPDFGLLTTTEVVGTVRRITAAAPNLCVVVDGDTGGGGPLNVQRFIKELIAAGAKGVFLE 120
           LPDFGLLTTTEVVGTVRRITAAAPNLCVVVDGDTGGGGPLNVQRFIKELIAAGAKGVFLE
Sbjct: 61  LPDFGLLTTTEVVGTVRRITAAAPNLCVVVDGDTGGGGPLNVQRFIKELIAAGAKGVFLE 120

Query: 121 DQVWPKKCGHMRGKSVVPAEEHALKIAAAREAIGDSDFFLVARSDARAPHGLQEAIRRAN 180
           DQVWPKKCGHMRGKSVVPAEEHALKIAAAREAIGDSDFFLVARSDARAPHGLQEAIRRAN
Sbjct: 121 DQVWPKKCGHMRGKSVVPAEEHALKIAAAREAIGDSDFFLVARSDARAPHGLQEAIRRAN 180

Query: 181 LYREAGADATFVEAPANLEELKEVVRDVKGLRIANMIEGGKTPLHTPAEFKEMGFHLIAH 240
           LYREAGADATFVEAPANLEELKEVVRDVKGLRIANMIEGGKTPLHTPAEFKEMGFHLIAH
Sbjct: 181 LYREAGADATFVEAPANLEELKEVVRDVKGLRIANMIEGGKTPLHTPAEFKEMGFHLIAH 240

Query: 241 SLSTIYATTKALVSIMKVLKDKGTTRDDLDQIVTFSEFNDMISLESWYELESKFKTFTPK 300
           SLSTIYATTKALVSIMKVLKDKGTTRDDLDQIVTFSEFNDMISLESWYELESKFKTFTPK
Sbjct: 241 SLSTIYATTKALVSIMKVLKDKGTTRDDLDQIVTFSEFNDMISLESWYELESKFKTFTPK 300

Query: 301 SLES 305
           SLES
Sbjct: 301 SLES 304

[Close]

BLAST of Spo21589.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QKY2_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_172700 PE=4 SV=1)

HSP 1 Score: 576.2 bits (1484), Expect = 2.300e-161
Identity = 294/304 (96.71%), Postives = 298/304 (98.03%), Query Frame = 1


		  
Query: 1   MGSLEAAAPLGRTTVHRLIAEEGSVLMPGIQDAFSAAICYKTGFKACFVSGFGVSAALLG 60
           MGSLEA+APLGRTTVHRLIAEEGSVLMPGIQDAFSAAIC KTGFKACFVSGFGVSAALLG
Sbjct: 1   MGSLEASAPLGRTTVHRLIAEEGSVLMPGIQDAFSAAICAKTGFKACFVSGFGVSAALLG 60

Query: 61  LPDFGLLTTTEVVGTVRRITAAAPNLCVVVDGDTGGGGPLNVQRFIKELIAAGAKGVFLE 120
           LPDFGLLTTTEVV TVRRITAAAP+LCVVVDGDTGGGGPLNVQRFIKELIAAGAKGVFLE
Sbjct: 61  LPDFGLLTTTEVVETVRRITAAAPDLCVVVDGDTGGGGPLNVQRFIKELIAAGAKGVFLE 120

Query: 121 DQVWPKKCGHMRGKSVVPAEEHALKIAAAREAIGDSDFFLVARSDARAPHGLQEAIRRAN 180
           DQVWPKKCGHMRGKSVVPAEEHALKIAAAREAIGDSDFFLVARSDARAPHGLQEAIRRAN
Sbjct: 121 DQVWPKKCGHMRGKSVVPAEEHALKIAAAREAIGDSDFFLVARSDARAPHGLQEAIRRAN 180

Query: 181 LYREAGADATFVEAPANLEELKEVVRDVKGLRIANMIEGGKTPLHTPAEFKEMGFHLIAH 240
           LYREAGADATFVEAPANLEELKEVVRDVKGLRIANMIEGGKTPLHTP EFKEMGFHLIAH
Sbjct: 181 LYREAGADATFVEAPANLEELKEVVRDVKGLRIANMIEGGKTPLHTPTEFKEMGFHLIAH 240

Query: 241 SLSTIYATTKALVSIMKVLKDKGTTRDDLDQIVTFSEFNDMISLESWYELESKFKTFTPK 300
           SLSTIYATTKALV IMKVLKDKGTTRDDL++IVTFSEFNDMISLESWYE ESKFK FTPK
Sbjct: 241 SLSTIYATTKALVDIMKVLKDKGTTRDDLEKIVTFSEFNDMISLESWYETESKFKNFTPK 300

Query: 301 SLES 305
           SLES
Sbjct: 301 SLES 304

[Close]

BLAST of Spo21589.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RFB8_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_073210 PE=4 SV=1)

HSP 1 Score: 548.1 bits (1411), Expect = 6.600e-153
Identity = 278/306 (90.85%), Postives = 289/306 (94.44%), Query Frame = 1


		  
Query: 1   MGSLEAA--APLGRTTVHRLIAEEGSVLMPGIQDAFSAAICYKTGFKACFVSGFGVSAAL 60
           MGSL       +GRTTVHRLIAEEGSVLMPGIQD FSAAICYKTGFKACFVSGFGVSAAL
Sbjct: 1   MGSLAETNGVAVGRTTVHRLIAEEGSVLMPGIQDPFSAAICYKTGFKACFVSGFGVSAAL 60

Query: 61  LGLPDFGLLTTTEVVGTVRRITAAAPNLCVVVDGDTGGGGPLNVQRFIKELIAAGAKGVF 120
           LGLPDFGLLTT EVVG VRRITAA+PNLCVVVDGDTGGGGPLNVQRFIK+LIAAGAKGVF
Sbjct: 61  LGLPDFGLLTTAEVVGVVRRITAASPNLCVVVDGDTGGGGPLNVQRFIKDLIAAGAKGVF 120

Query: 121 LEDQVWPKKCGHMRGKSVVPAEEHALKIAAAREAIGDSDFFLVARSDARAPHGLQEAIRR 180
           LEDQVWPKKCGHMRGK+VVP EEHALKIAAAREAIGDSDFFLVAR+DARAPHGLQEAIRR
Sbjct: 121 LEDQVWPKKCGHMRGKAVVPVEEHALKIAAAREAIGDSDFFLVARTDARAPHGLQEAIRR 180

Query: 181 ANLYREAGADATFVEAPANLEELKEVVRDVKGLRIANMIEGGKTPLHTPAEFKEMGFHLI 240
           AN+YREAGADATFVEAPAN EEL EVV+ VKGLRIANMIEGGKTPLHTP EFKEMGFHLI
Sbjct: 181 ANIYREAGADATFVEAPANREELDEVVKGVKGLRIANMIEGGKTPLHTPGEFKEMGFHLI 240

Query: 241 AHSLSTIYATTKALVSIMKVLKDKGTTRDDLDQIVTFSEFNDMISLESWYELESKFKTFT 300
           AHSLST+YATTKALV IMKVLKDKGTTRDDLD++V+FSEFNDMISLESWYELESKFK FT
Sbjct: 241 AHSLSTLYATTKALVDIMKVLKDKGTTRDDLDKMVSFSEFNDMISLESWYELESKFKNFT 300

Query: 301 PKSLES 305
           PKSLES
Sbjct: 301 PKSLES 306

[Close]

BLAST of Spo21589.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RFD0_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_073200 PE=4 SV=1)

HSP 1 Score: 546.2 bits (1406), Expect = 2.500e-152
Identity = 277/305 (90.82%), Postives = 288/305 (94.43%), Query Frame = 1


		  
Query: 1   MGSL-EAAAPLGRTTVHRLIAEEGSVLMPGIQDAFSAAICYKTGFKACFVSGFGVSAALL 60
           MGSL E  A +G+TTVHRLIAEEGSVLMPGIQDAFSAAICYKTGFKACFVSGFGVSAALL
Sbjct: 1   MGSLSEGNAAMGKTTVHRLIAEEGSVLMPGIQDAFSAAICYKTGFKACFVSGFGVSAALL 60

Query: 61  GLPDFGLLTTTEVVGTVRRITAAAPNLCVVVDGDTGGGGPLNVQRFIKELIAAGAKGVFL 120
           GLPDFGLLTT EVV  VRRITA +PNLCVVVDGDTGGGGPLNVQRFIK+LI AGAKGVFL
Sbjct: 61  GLPDFGLLTTAEVVEVVRRITAGSPNLCVVVDGDTGGGGPLNVQRFIKDLINAGAKGVFL 120

Query: 121 EDQVWPKKCGHMRGKSVVPAEEHALKIAAAREAIGDSDFFLVARSDARAPHGLQEAIRRA 180
           EDQVWPKKCGHMRGK+VVP EEHALKIAAAREAIGDSDFFLVAR+DARAPHGLQEAIRRA
Sbjct: 121 EDQVWPKKCGHMRGKAVVPVEEHALKIAAAREAIGDSDFFLVARTDARAPHGLQEAIRRA 180

Query: 181 NLYREAGADATFVEAPANLEELKEVVRDVKGLRIANMIEGGKTPLHTPAEFKEMGFHLIA 240
           N+YREAGADATFVEAPAN EEL EVV+ VKGLRIANMIEGGKTPLHTP EFKEMGFHLIA
Sbjct: 181 NIYREAGADATFVEAPANREELDEVVKGVKGLRIANMIEGGKTPLHTPGEFKEMGFHLIA 240

Query: 241 HSLSTIYATTKALVSIMKVLKDKGTTRDDLDQIVTFSEFNDMISLESWYELESKFKTFTP 300
           HSLST+YATTKALV IMKVLKDKGTTRDD+D+IVTFSEFNDMISLESWYE ESKFK FTP
Sbjct: 241 HSLSTLYATTKALVDIMKVLKDKGTTRDDMDKIVTFSEFNDMISLESWYETESKFKNFTP 300

Query: 301 KSLES 305
           KSLES
Sbjct: 301 KSLES 305

[Close]

BLAST of Spo21589.1 vs. UniProtKB/TrEMBL
Match: A0A0J8BDW4_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_1g023220 PE=4 SV=1)

HSP 1 Score: 538.9 bits (1387), Expect = 4.000e-150
Identity = 272/302 (90.07%), Postives = 290/302 (96.03%), Query Frame = 1


		  
Query: 1   MGSLEAA-APLGRTTVHRLIAEEGSVLMPGIQDAFSAAICYKTGFKACFVSGFGVSAALL 60
           MGSL+A+ A  GRTTVHRLIAEEG++LMPGIQDAFSAAIC KTG KACFVSGFGVSAALL
Sbjct: 1   MGSLDASNAVEGRTTVHRLIAEEGALLMPGIQDAFSAAICAKTGHKACFVSGFGVSAALL 60

Query: 61  GLPDFGLLTTTEVVGTVRRITAAAPNLCVVVDGDTGGGGPLNVQRFIKELIAAGAKGVFL 120
           GLPDFGLLTT EVV  VRRITAAAP+LCVVVDGDTGGGGPLNVQRFIK+LIAAGAKGVFL
Sbjct: 61  GLPDFGLLTTAEVVDVVRRITAAAPSLCVVVDGDTGGGGPLNVQRFIKDLIAAGAKGVFL 120

Query: 121 EDQVWPKKCGHMRGKSVVPAEEHALKIAAAREAIGDSDFFLVARSDARAPHGLQEAIRRA 180
           EDQVWPKKCGHMRGK VVPAEEHALKIAAAR+AIGDSDFFLVARSDARAPHGLQEAIRRA
Sbjct: 121 EDQVWPKKCGHMRGKMVVPAEEHALKIAAARDAIGDSDFFLVARSDARAPHGLQEAIRRA 180

Query: 181 NLYREAGADATFVEAPANLEELKEVVRDVKGLRIANMIEGGKTPLHTPAEFKEMGFHLIA 240
           NLYRE+GADATFVEAPANLEELKEVVRDVKGLRIANMIEGGKTPLHTPAEFKEMGFHLIA
Sbjct: 181 NLYRESGADATFVEAPANLEELKEVVRDVKGLRIANMIEGGKTPLHTPAEFKEMGFHLIA 240

Query: 241 HSLSTIYATTKALVSIMKVLKDKGTTRDDLDQIVTFSEFNDMISLESWYELESKFKTFTP 300
           HSLST+YATTKAL+ I++ LK+KGTTRDD DQ+VTF+EFNDMISLESWYELE+KFK+FTP
Sbjct: 241 HSLSTLYATTKALMGILRTLKEKGTTRDDFDQMVTFNEFNDMISLESWYELETKFKSFTP 300

Query: 301 KS 302
           K+
Sbjct: 301 KA 302

[Close]

BLAST of Spo21589.1 vs. ExPASy Swiss-Prot
Match: PDP_DIACA (Petal death protein OS=Dianthus caryophyllus GN=PDP PE=1 SV=1)

HSP 1 Score: 499.2 bits (1284), Expect = 3.200e-140
Identity = 247/300 (82.33%), Postives = 273/300 (91.00%), Query Frame = 1


		  
Query: 2   GSLEAAAPLGRTTVHRLIAEEGSVLMPGIQDAFSAAICYKTGFKACFVSGFGVSAALLGL 61
           GS  A +   +TT+HRLI E GSVLMPG+QDA SAA+  KTGF A FVSG+ VSAA+LGL
Sbjct: 18  GSYTAVSTGRKTTMHRLIEEHGSVLMPGVQDALSAAVVEKTGFHAAFVSGYSVSAAMLGL 77

Query: 62  PDFGLLTTTEVVGTVRRITAAAPNLCVVVDGDTGGGGPLNVQRFIKELIAAGAKGVFLED 121
           PDFGLLTTTEVV   RRITAAAPNLCVVVDGDTGGGGPLNVQRFI+ELI+AGAKGVFLED
Sbjct: 78  PDFGLLTTTEVVEATRRITAAAPNLCVVVDGDTGGGGPLNVQRFIRELISAGAKGVFLED 137

Query: 122 QVWPKKCGHMRGKSVVPAEEHALKIAAAREAIGDSDFFLVARSDARAPHGLQEAIRRANL 181
           QVWPKKCGHMRGK+VVPAEEHALKIAAAREAIGDSDFFLVAR+DARAPHGL+E IRRANL
Sbjct: 138 QVWPKKCGHMRGKAVVPAEEHALKIAAAREAIGDSDFFLVARTDARAPHGLEEGIRRANL 197

Query: 182 YREAGADATFVEAPANLEELKEVVRDVKGLRIANMIEGGKTPLHTPAEFKEMGFHLIAHS 241
           Y+EAGADATFVEAPAN++ELKEV    KGLRIANMIEGGKTPLHTP EFKEMGFHLIAHS
Sbjct: 198 YKEAGADATFVEAPANVDELKEVSAKTKGLRIANMIEGGKTPLHTPEEFKEMGFHLIAHS 257

Query: 242 LSTIYATTKALVSIMKVLKDKGTTRDDLDQIVTFSEFNDMISLESWYELESKFKTFTPKS 301
           L+ +YAT +ALV+IMK+LK+KGTTRDDLDQ+ TFSEFN++ISLESWYE+ESKFK FTPK+
Sbjct: 258 LTAVYATARALVNIMKILKEKGTTRDDLDQMATFSEFNELISLESWYEMESKFKNFTPKA 317

[Close]

BLAST of Spo21589.1 vs. ExPASy Swiss-Prot
Match: CPPM_ARATH (Carboxyvinyl-carboxyphosphonate phosphorylmutase, chloroplastic OS=Arabidopsis thaliana GN=At1g77060 PE=2 SV=2)

HSP 1 Score: 371.3 bits (952), Expect = 1.000e-101
Identity = 179/286 (62.59%), Postives = 224/286 (78.32%), Query Frame = 1


		  
Query: 12  RTTVHRLIAEEGSVLMPGIQDAFSAAICYKTGFKACFVSGFGVSAALLGLPDFGLLTTTE 71
           +T  HRLI E+G VLMPG  DA SAAI  +TGF A F+SG+ +SA+LLG PDFGLLT  E
Sbjct: 42  QTRFHRLIEEQGIVLMPGCYDALSAAIVQQTGFSAGFISGYALSASLLGKPDFGLLTPPE 101

Query: 72  VVGTVRRITAAAPNLCVVVDGDTGGGGPLNVQRFIKELIAAGAKGVFLEDQVWPKKCGHM 131
           +  T R + A+APN+ ++ D DTGGG  LN+QR +K+LIAAGA G FLEDQ WPKKCGHM
Sbjct: 102 MAATARSVCASAPNIPIIADADTGGGNALNIQRTVKDLIAAGAAGCFLEDQAWPKKCGHM 161

Query: 132 RGKSVVPAEEHALKIAAAREAIGDSDFFLVARSDAR---APHGLQEAIRRANLYREAGAD 191
           RGK V+PAEEHA KIA+AR+AIGDSDFFLVAR+D R   A  GL++AI R NLY EAGAD
Sbjct: 162 RGKQVIPAEEHAAKIASARDAIGDSDFFLVARTDVRATSAKSGLEDAIARVNLYMEAGAD 221

Query: 192 ATFVEAPANLEELKEVVRDVKGLRIANMIEGGKTPLHTPAEFKEMGFHLIAHSLSTIYAT 251
           A+FVEAP + +ELKE+ +  KG R+ NMIEGG TPLHTP E KEMGFHLI H L+ +YA+
Sbjct: 222 ASFVEAPRDDDELKEIGKRTKGYRVCNMIEGGVTPLHTPDELKEMGFHLIVHPLTALYAS 281

Query: 252 TKALVSIMKVLKDKGTTRDDLDQIVTFSEFNDMISLESWYELESKF 295
           T+ALV ++K LK+ G+TRD L ++ TF EFN ++ L+SW+ELE+++
Sbjct: 282 TRALVDVLKTLKENGSTRDHLQKMATFEEFNSLVDLDSWFELEARY 327

[Close]

BLAST of Spo21589.1 vs. ExPASy Swiss-Prot
Match: DML_EUBBA (2,3-dimethylmalate lyase OS=Eubacterium barkeri GN=Dml PE=1 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 8.000e-59
Identity = 120/282 (42.55%), Postives = 175/282 (62.06%), Query Frame = 1


		  
Query: 15  VHRLIAEEGSVLMPGIQDAFSAAICYKTGFKACFVSGFGVSAALLGLPDFGLLTTTEVVG 74
           +  L++ +  V+ PG  DA +A +  + GF A +++G+G SA+ LG PD GLLT TE+V 
Sbjct: 7   MRELLSTKKMVVAPGAHDAMTAKVIGRLGFDAVYMTGYGQSASHLGQPDVGLLTMTEMVA 66

Query: 75  TVRRITAAAPNLCVVVDGDTGGGGPLNVQRFIKELIAAGAKGVFLEDQVWPKKCGHMRGK 134
               I  AA  + V+ D DTG G  +NV R ++E   AG   + LEDQV PKKCGHM G+
Sbjct: 67  RANAIVEAA-GVPVIADADTGFGNAVNVMRTVREYEKAGVAVIQLEDQVMPKKCGHMVGR 126

Query: 135 SVVPAEEHALKIAAAREAIGDSDFFLVARSDARAPHGLQEAIRRANLYREAGADATFVEA 194
            +V  EE   KI AA +   + DF ++AR+DAR   G+ EA+ R   Y+EAGAD  F+E+
Sbjct: 127 EIVSKEEMVGKIKAAVDTRVNPDFMIMARTDARTTKGIDEALERGLAYKEAGADIIFIES 186

Query: 195 PANLEELKEVVRDVKGLRIANMIEGGKTPLHTPAEFKEMGFHLIAHSLSTIYATTKALVS 254
           P   EE+K +   + G  +ANM+EGG+TPL   AE + +G+++  +  ++IY  TKA+V 
Sbjct: 187 PEGEEEMKRINETIPGYTLANMVEGGRTPLLKNAELEALGYNITIYPTASIYVATKAMVD 246

Query: 255 IMKVLKDKGTTRDDLDQIVTFSEFNDMISLESWYELESKFKT 297
           +   LK+  TT   +D +VTFSEFND++ LE   E+E  + T
Sbjct: 247 LWTALKNDDTTAGVMDTMVTFSEFNDLMGLEKIREVEHNYAT 287

[Close]

BLAST of Spo21589.1 vs. ExPASy Swiss-Prot
Match: PRPB_AERPE (2-methylisocitrate lyase OS=Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1) GN=prpB PE=3 SV=2)

HSP 1 Score: 194.1 bits (492), Expect = 2.200e-48
Identity = 108/279 (38.71%), Postives = 172/279 (61.65%), Query Frame = 1


		  
Query: 15  VHRLIAEEGSVLMPGIQDAFSAAICYKTGFKACFVSGFGVSAALLGLPDFGLLTTTEVVG 74
           +  LI +   V+ PG+ +   A +  + GF+A ++SG  ++ +L  +PD GL+T +E+  
Sbjct: 16  LRELIEKRDIVVAPGVYNPAVALLAERMGFEALYLSGAAITGSL-AMPDLGLITLSELAM 75

Query: 75  TVRRITAAAPNLCVVVDGDTGGGGPLNVQRFIKELIAAGAKGVFLEDQVWPKKCGHMRGK 134
               IT     + V+VD DTG G  +NV+R ++EL  AGA  + +EDQV PKKCGH++GK
Sbjct: 76  FTSYITRVV-RVPVIVDADTGFGEAINVERTVRELERAGAAAIQIEDQVMPKKCGHLQGK 135

Query: 135 SVVPAEEHALKIAAAREAIGDSDFFLVARSDARAPHGLQEAIRRANLYREAGADATFVEA 194
           +++  E+   KI AA  A  D+   +VAR+DAR   G ++A+ RA LY EAGAD  F EA
Sbjct: 136 ALISPEDMVKKIIAAVGARRDA--LIVARTDARGVEGFEKAVERAQLYVEAGADIIFPEA 195

Query: 195 PANLEELKEVVRDVKGLRIANMIEGGKTPLHTPAEFKEMGFHLIAHSLSTIYATTKALVS 254
             +LEE +E  R VK   +ANM E GKTP  T  +F+E G+ ++   ++T  A+ KA  +
Sbjct: 196 LTSLEEFREFARRVKAPLLANMTEFGKTPYITVDQFREAGYKIVIFPVTTFRASLKASET 255

Query: 255 IMKVLKDKGTTRDDLDQIVTFSEFNDMISLESWYELESK 294
           +++ + +KGT +D LD++ T +EF D+I    + + +++
Sbjct: 256 VLREIMEKGTQKDILDKLYTRTEFYDLIGYHDYEKRDAE 290

[Close]

BLAST of Spo21589.1 vs. ExPASy Swiss-Prot
Match: PRPB_BACHD (2-methylisocitrate lyase OS=Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) GN=prpB PE=3 SV=1)

HSP 1 Score: 188.7 bits (478), Expect = 9.200e-47
Identity = 111/275 (40.36%), Postives = 161/275 (58.55%), Query Frame = 1


		  
Query: 17  RLIAEEGSVLMPGIQDAFSAAICYKTGFKACFVSGFGVSAALLGLPDFGLLTTTEVVGTV 76
           +L+ EE  + +PG  DA +A +  K GF A ++SG   +A+  GLPD G++T+TEV    
Sbjct: 20  QLMKEEAILQIPGAHDAMAALVAKKAGFSALYLSGDAYTASR-GLPDLGIVTSTEVADRA 79

Query: 77  RRITAAAPNLCVVVDGDTGGGGPLNVQRFIKELIAAGAKGVFLEDQVWPKKCGHMRGKSV 136
           + +  A  NL V+VD DTG GG LNV R  +E++ A    V +EDQ  PKKCGH+ GK +
Sbjct: 80  KDLVRAT-NLPVLVDIDTGFGGVLNVARTAQEMLEANVAAVQIEDQQLPKKCGHLNGKQL 139

Query: 137 VPAEEHALKIAAAREAIGDSDFFLVARSDARAPHGLQEAIRRANLYREAGADATFVEAPA 196
           V  EE   KI A ++        +VAR+DARA  GL  AI RAN+Y EAGADA F EA  
Sbjct: 140 VSKEEMEQKIQAIKKVA--PTLVIVARTDARANEGLNGAIERANVYIEAGADAIFPEALQ 199

Query: 197 NLEELKEVVRDVKGLRIANMIEGGKTPLHTPAEFKEMGFHLIAHSLSTIYATTKALVSIM 256
           + EE + V  +V    +ANM E GKTPL T    +  GF ++ + ++++    KA   I 
Sbjct: 200 SAEEFRLVAENVSAPLLANMTEFGKTPLMTAGGLQNAGFQMVIYPVTSLRVAAKAYERIF 259

Query: 257 KVLKDKGTTRDDLDQIVTFSEFNDMISLESWYELE 292
           +++KD+GT    ++ + T  E  + IS + +  L+
Sbjct: 260 QLIKDEGTQEAGIEDMQTRKELYETISYDDFEALD 290

[Close]

BLAST of Spo21589.1 vs. TAIR (Arabidopsis)
Match: AT1G21440.1 (Phosphoenolpyruvate carboxylase family protein)

HSP 1 Score: 377.9 bits (969), Expect = 6.000e-105
Identity = 179/286 (62.59%), Postives = 232/286 (81.12%), Query Frame = 1


		  
Query: 12  RTTVHRLIAEEGSVLMPGIQDAFSAAICYKTGFKACFVSGFGVSAALLGLPDFGLLTTTE 71
           ++ VHRLI E+G+VL+PG+ DA SAAI  +TGF A  +SG+ +SA  LG PDFGL+T  E
Sbjct: 40  QSRVHRLIEEQGAVLIPGVYDALSAAIVQQTGFSAALISGYALSAVTLGKPDFGLITPPE 99

Query: 72  VVGTVRRITAAAPNLCVVVDGDTGGGGPLNVQRFIKELIAAGAKGVFLEDQVWPKKCGHM 131
           +  T R + AAAP + ++ D DTGGG  LNVQR +K+LIAAGA G FLEDQ WPK+CGHM
Sbjct: 100 MAATARSVCAAAPKIPIIADADTGGGNALNVQRTVKDLIAAGAAGCFLEDQAWPKRCGHM 159

Query: 132 RGKSVVPAEEHALKIAAAREAIGDSDFFLVARSDAR---APHGLQEAIRRANLYREAGAD 191
           RGK V+PAEEHA KIA+AR+AIGD+DFFL+AR+DAR   A  GL +AI RANLY EAGAD
Sbjct: 160 RGKEVIPAEEHAAKIASARDAIGDADFFLIARTDARALSAKTGLSDAIDRANLYMEAGAD 219

Query: 192 ATFVEAPANLEELKEVVRDVKGLRIANMIEGGKTPLHTPAEFKEMGFHLIAHSLSTIYAT 251
           A+FVEAP + +ELKE+ R  KG R+ NM+EGG+TPLHTP E KEMGFHLIAH L+++YA+
Sbjct: 220 ASFVEAPRDDDELKEIGRRTKGYRLCNMLEGGRTPLHTPDELKEMGFHLIAHPLTSLYAS 279

Query: 252 TKALVSIMKVLKDKGTTRDDLDQIVTFSEFNDMISLESWYELESKF 295
           T+ALV ++K+LK+KGTT+D L++++TF EFN +++L+SWYELE+K+
Sbjct: 280 TRALVDVLKILKEKGTTKDHLEKMITFEEFNRLVNLDSWYELETKY 325

[Close]

BLAST of Spo21589.1 vs. TAIR (Arabidopsis)
Match: AT1G77060.1 (Phosphoenolpyruvate carboxylase family protein)

HSP 1 Score: 371.3 bits (952), Expect = 5.600e-103
Identity = 179/286 (62.59%), Postives = 224/286 (78.32%), Query Frame = 1


		  
Query: 12  RTTVHRLIAEEGSVLMPGIQDAFSAAICYKTGFKACFVSGFGVSAALLGLPDFGLLTTTE 71
           +T  HRLI E+G VLMPG  DA SAAI  +TGF A F+SG+ +SA+LLG PDFGLLT  E
Sbjct: 42  QTRFHRLIEEQGIVLMPGCYDALSAAIVQQTGFSAGFISGYALSASLLGKPDFGLLTPPE 101

Query: 72  VVGTVRRITAAAPNLCVVVDGDTGGGGPLNVQRFIKELIAAGAKGVFLEDQVWPKKCGHM 131
           +  T R + A+APN+ ++ D DTGGG  LN+QR +K+LIAAGA G FLEDQ WPKKCGHM
Sbjct: 102 MAATARSVCASAPNIPIIADADTGGGNALNIQRTVKDLIAAGAAGCFLEDQAWPKKCGHM 161

Query: 132 RGKSVVPAEEHALKIAAAREAIGDSDFFLVARSDAR---APHGLQEAIRRANLYREAGAD 191
           RGK V+PAEEHA KIA+AR+AIGDSDFFLVAR+D R   A  GL++AI R NLY EAGAD
Sbjct: 162 RGKQVIPAEEHAAKIASARDAIGDSDFFLVARTDVRATSAKSGLEDAIARVNLYMEAGAD 221

Query: 192 ATFVEAPANLEELKEVVRDVKGLRIANMIEGGKTPLHTPAEFKEMGFHLIAHSLSTIYAT 251
           A+FVEAP + +ELKE+ +  KG R+ NMIEGG TPLHTP E KEMGFHLI H L+ +YA+
Sbjct: 222 ASFVEAPRDDDELKEIGKRTKGYRVCNMIEGGVTPLHTPDELKEMGFHLIVHPLTALYAS 281

Query: 252 TKALVSIMKVLKDKGTTRDDLDQIVTFSEFNDMISLESWYELESKF 295
           T+ALV ++K LK+ G+TRD L ++ TF EFN ++ L+SW+ELE+++
Sbjct: 282 TRALVDVLKTLKENGSTRDHLQKMATFEEFNSLVDLDSWFELEARY 327

[Close]

BLAST of Spo21589.1 vs. TAIR (Arabidopsis)
Match: AT2G43180.1 (Phosphoenolpyruvate carboxylase family protein)

HSP 1 Score: 135.6 bits (340), Expect = 5.200e-32
Identity = 91/287 (31.71%), Postives = 159/287 (55.40%), Query Frame = 1


		  
Query: 15  VHRLIAEEGSVLMPGIQDAFSAAICYKTGFKACFVSGFGVSAALLGLPDFGLLTTTEVVG 74
           +  ++   G +  P   DA SA +  + GF  C  SGF +SA+ LGLPD GL++  E+V 
Sbjct: 76  LRNIMQSPGVLQGPCCFDALSAKLIERAGFPYCITSGFSISASRLGLPDKGLISYGEMVD 135

Query: 75  TVRRITAAAPNLCVVVDGDTGGGGPLNVQRFIKELIAAGAKGVFLEDQVWPKKCGHMRG- 134
             ++IT +  ++ V+ DG  G G  +NV+R +K  I AG  G+ + D+V    C + +  
Sbjct: 136 QGQQITQSV-SIPVIGDGGNGYGNAMNVKRTVKGYIKAGFAGIIINDEVC---CENTKSE 195

Query: 135 KSVVPAEEHALKIAAAREAIG--DSDFFLVARSDARAPHGLQEAIRRANLYREAGADATF 194
           + VV  EE  +++ AA +A    DSD  +VA++D+R    L+E++ RA  + +AGAD   
Sbjct: 196 RRVVSREEAVMRVKAAVDARRECDSDIVIVAQTDSREAISLEESLIRARAFTDAGADVLS 255

Query: 195 VEAPANLEELKEVVRDVKGL-RIANMIE-GGKTPLHTPAEFKEMGFHLIAHSLSTIYATT 254
           V++  + EE+K        + ++ANM+E GGK P+  P E +E+G+ L+A+ +S I  + 
Sbjct: 256 VDSLVSREEMKAFCNVYPLVPKLANMLESGGKIPILNPLELEEIGYKLVAYPISLIGVSI 315

Query: 255 KALVSIMKVLKDKGTTRDDLDQIVTFSEFNDMISLESWYELESKFKT 297
           +A+   +  L  KG        + +  E  +++  +++ E E ++ T
Sbjct: 316 QAMQDAL--LAIKGGRIPPPGSMASLEEIGEILGFDTYEEEEKRYAT 356

[Close]

BLAST of Spo21589.1 vs. TAIR (Arabidopsis)
Match: AT3G21720.1 (isocitrate lyase)

HSP 1 Score: 62.4 bits (150), Expect = 5.600e-10
Identity = 39/93 (41.94%), Postives = 53/93 (56.99%), Query Frame = 1


		  
Query: 88  VVVDGDTGGGGPLNVQRFIKELIAAGAKGVFLEDQVW-PKKCGHMRGKSVVPAEEHALKI 147
           ++ DGDTG GG     +  K  +  GA GV +EDQ    KKCGHM GK +V   EH  ++
Sbjct: 172 IIADGDTGFGGTTATVKLCKLFVERGAAGVHIEDQSSVTKKCGHMAGKVLVAVSEHINRL 231

Query: 148 AAAR---EAIGDSDFFLVARSDARAPHGLQEAI 177
            AAR   + +G ++  LVAR+DA A   +Q  I
Sbjct: 232 VAARLQFDVMG-TETVLVARTDAVAATLIQSNI 263

The following BLAST results are available for this feature:

BLAST of Spo21589.1 vs. NCBI nr
BLAST of Spo21589.1 vs. UniProtKB/TrEMBL
BLAST of Spo21589.1 vs. ExPASy Swiss-Prot
BLAST of Spo21589.1 vs. TAIR (Arabidopsis)

BLAST of Spo21589.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902167267\|gb\|KNA07347.1\|	4.0e-167	100.	hypothetical protein SOVF_1726...	[more]
gi\|902167268\|gb\|KNA07348.1\|	3.3e-161	96.7	hypothetical protein SOVF_1727...	[more]
gi\|902218930\|gb\|KNA18181.1\|	9.5e-153	90.8	hypothetical protein SOVF_0732...	[more]
gi\|902218929\|gb\|KNA18180.1\|	3.6e-152	90.8	hypothetical protein SOVF_0732...	[more]
gi\|731362409\|ref\|XP_010692873.1\|	5.8e-150	90.0	PREDICTED: petal death protein...	[more]

BLAST of Spo21589.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9QJB6_SPIOL	2.8e-167	100.	Uncharacterized protein OS=Spi...	[more]
A0A0K9QKY2_SPIOL	2.3e-161	96.7	Uncharacterized protein OS=Spi...	[more]
A0A0K9RFB8_SPIOL	6.6e-153	90.8	Uncharacterized protein OS=Spi...	[more]
A0A0K9RFD0_SPIOL	2.5e-152	90.8	Uncharacterized protein OS=Spi...	[more]
A0A0J8BDW4_BETVU	4.0e-150	90.0	Uncharacterized protein OS=Bet...	[more]

BLAST of Spo21589.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5


Match Name	E-value	Identity	Description
PDP_DIACA	3.2e-140	82.3	Petal death protein OS=Dianthu...	[more]
CPPM_ARATH	1.0e-101	62.5	Carboxyvinyl-carboxyphosphonat...	[more]
DML_EUBBA	8.0e-59	42.5	2,3-dimethylmalate lyase OS=Eu...	[more]
PRPB_AERPE	2.2e-48	38.7	2-methylisocitrate lyase OS=Ae...	[more]
PRPB_BACHD	9.2e-47	40.3	2-methylisocitrate lyase OS=Ba...	[more]

BLAST of Spo21589.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 4


Match Name	E-value	Identity	Description
AT1G21440.1	6.0e-105	62.5	Phosphoenolpyruvate carboxylas...	[more]
AT1G77060.1	5.6e-103	62.5	Phosphoenolpyruvate carboxylas...	[more]
AT2G43180.1	5.2e-32	31.7	Phosphoenolpyruvate carboxylas...	[more]
AT3G21720.1	5.6e-10	41.9	isocitrate lyase	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR015813	Pyruvate/Phosphoenolpyruvate kinase-like domain	GENE3D	3.20.20.60		coord: 14..295 score: 4.2
IPR015813	Pyruvate/Phosphoenolpyruvate kinase-like domain	unknown	SSF51621	Phosphoenolpyruvate/pyruvate domain	coord: 13..296 score: 2.33
IPR018523	Isocitrate lyase/phosphorylmutase, conserved site	PROSITE	PS00161	ISOCITRATE_LYASE	coord: 126..131 scor
None	No IPR available	PANTHER	PTHR21631	ISOCITRATE LYASE/MALATE SYNTHASE	coord: 13..293 score: 4.6E
None	No IPR available	PANTHER	PTHR21631:SF4	CARBOXYVINYL-CARBOXYPHOSPHONATE PHOSPHORYLMUTASE, CHLOROPLASTIC	coord: 13..293 score: 4.6E
None	No IPR available	PFAM	PF13714	PEP_mutase	coord: 18..257 score: 2.1

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008152	metabolic process
biological_process	GO:0019761	glucosinolate biosynthetic process
biological_process	GO:0006097	glyoxylate cycle
cellular_component	GO:0009570	chloroplast stroma
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0047776	citramalate lyase activity
molecular_function	GO:0004451	isocitrate lyase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0030603	oxaloacetase activity
molecular_function	GO:0016740	transferase activity