Spo22007.1 (mRNA) | SpinachBase

Overview


Name	Spo22007.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	(Sirohydrochlorin ferrochelatase, putative) (4.99.1.3)
Location	chr1 : 12217334 .. 12220709 (+)
Sequence length	1313

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGCCCTGGTGTTACGAAGGTTTCAGATAAAACTTCCCATCCATGTAACCAAAGTGCGGAGTTTTTGTTGGTTGTTAAAGTAATTATATTAAGTTGACCCCGATCTCATTTGGTTTGCAGCTAAATAGTGGTGGGTTTTTTCTTGTTGGGATTTTTGGGTTAATATTATTGCTCCTGTTTAATCTTCCCAGCTGATGAATAGATGATAAGCACGATTAACAAGAACTCAAATGTCACTAAAGTTCTTGTCTTGTAATCAGTGGGTTCACCAGCTTCACCTTGAAAGGTATTTGATATTGAGACTCTTTTACCTAGGCATTCTCCCTTTACTCTAAAATTATTTTCACGCTCTTATTGCCCTTTTCCTTCTATTCTATGGTGGTCAGCCCGGTGTCATTAACTACCGCCAAAGGAAGTCGGATAGAAGTTGGACCTAGATTTCTTAGACTACCACAAAACACTCAACATAAGAGCCTGTCTACAAGTTTATGCTTTGCTATGGATCAGAAAATCGTGCATGAGGGTGCCTTTGGTGTTAGTCAGAAAGATGGTGTGATTATCGTTGATCATGGATCTCGTCGGAAAGAGTCCAATCTCTTACTGAGTAATGCTATTTAGAGTTGTTCATTTTCTTTGTCAATGCCTTTCCTGGGTAGACCTAGGAGCTATTTATGCAACTCTCTGTATTCTCTATTTCATTATGCAACGGTGTGCTTACTTGTTGTGTACTGCAGATGAATTTGTGCTGATGTTCAAAAGCAAAACTGGATACCCTATTGTTGAGCCGGCTCATATGGTACTCCCTACTTTTAGATATCATTATGCAATGTTTTCGAAAAAAAATTTAGGTGCACCCTGGTACACCGTACACCCAAGGGTACTTTTATGCCCATTGGTCGTTCCTCTAACATTTTCTCCCGACACGTAAGGGGAAAATGTGAGAGGGTGCACCTCAGGTGTGCTGATGTTTTCGTGATGGAGATGAGATTTCTGATTGAATCTGGAGCCAATCTCCATCGCCTTTCTGTTGTGTTGTGCAATTGGTGCACTTGTACAAACTAGAAAGATGCCAATTAAGACAATGATGGATCTGAAGTTTGGAACAGATAGTCATCATTTAAAACTGTCATTATGCAATGAATTTTTCTGTTCAATGTTTAGGAATTGGCAGAACCATCCATAAAAGATGCTTTCAATTCATGTGTCGAGCAAGGGGCAAATCGTGTTATCATAAGCCCATTCTTCCTATTTCCTGGAAGGCATTGGCATCAGGTAAGAAAACTAAATCACTCTGCTGCTTCTGCACTTTGATCACTATTTGCGAACTTATCGGTCATGGGGTACCCCCCCAAACACACACACACACACTCCTCTTCTTCCTAAAAACAAAGGAAGCCGCTGAAGCGTAAAAATAAAGTGACAGGAGTTTTTTGCATGTTGCAGTTGTTATGCCCTTTGTGTGCCTTTAATTGCAGGCTACTACATTACATTAGAATTTCATACAAGAATGTAACTTATCCTATCTCATGAAAGGGAAAGTAAATGATCCAGGGAAGGATGTTGATTCACTTTTAGGAAGGTTAAAGAGTCTGATGGAACCTTTCGATTTCTGAACATAAAATTTGAAACTGGCAATTTAAACAGGATTGCTATTGTTGTTGTACAGGATATTCCTTCCCTTGCAGATGCTGCTGCTCTGGAGCATCCCGGTATCAATTATCTTGTCACTGCACCGTTGGGTCTGCATGAGTTACTTGTGGTATTAACTGCATCCCTTCTAACTAGTATGATTTTATCTTTACTGCCCCTGTCTGCGTTTGGAAATTTTCAAGTGGTGAAAAGACATGGTGGTTATAAATGTTGTCTATTCTATTTTGTGTTTCATCTACGCATTACACATATTCACCCATCCAACCTCACCCGATTCAGATAGTGAACTGAAGTGATTTGTTACTTTCTTGATACCACCTGTGGCAAATATACGAAGTATAATTTATTGAACTTCAACTCTTCCCAGTGTTGTACAACTGCTGAATTCACTTGTTAGCTACTATTATCTTGAGATGATTGGATGATCATCACGCTGAAATAAGTTTTCTAAGTGACATTTAAGGCAGAATATATTGATTTTAATCTTGTGTGTTACGGTATCTGATCTTGCAGGATGTTGTGAAGGACAGAATTGACTATTGCTTAAGTCATGTAGCTGGAAAAGTGGAGGAATGTGCAGTCTGTGCTGGAACAGGCAGGTGTAAATTTAAAGTGCAGGATTCCGAAAATTAATGTGTAGTGATTTAAGCTACGCCGCACTTGTCAGTTTCATGGATCAAGCTAGATAGCATGTCTTTAGAACGTCAACTGTAATAACCGGAAGGTATGGCATTGCTATTTGGAGTACACATCACTTCCTGAGAAATATCTGCAGCATATGCCGTGGGTGCTGAATATCTGGATGTAGTGGCTTGTTTTCTCAGTGTATATGACAATCAATCTGAGTGGATCACGTGATAAATAAGTATTGTAACATCATCATGAATTCATGACAATGGTAATCTATGTACTGCATAAGACGGTCAGTGAAGAATTTTGCCATTTTTTTGAAAGGGTGACGAATGTAGCAGTTGTAGCATTTTTTTTATTTTTTTTTACATAAACTTAATTTGCATAAATAAAGAAATCGTACATATTACAACTTATTACTACTAATTTACTAAAACACCTTAATCTATTAGGGCCATTATAACCCCTTACATAGATTACACACCGGTAGCATTTCATATGTTCAACAAAACTTCATCACTCACTTCAATTCTTCAACATACTCGTCAAAAATTAGTTTGCCATTGATTTGATCATCCTTTGTTTAAAAAAAATTCATTTTGACTGTCACAATTAGAAAATTAGAAGAAGAAAAAAATGTTGACATTTGGAAAATGTATATTGAGACTTAAATAACATTTGACATTAAAACTTTTGATTTTAATATGGTTAAAGTGATATAAGAAATGCAAAAGTGAAAGTCATGTTGTTACTTTGAAATAAAGTTGTAAACTCTAGTGGTAACATTGCATATCAGGGGGGACTTAGATAGGTTGAGCAATGCAGAAGAGGAGGAAGGTCAGTCTTCCAGAAGTAAGAGTGCAGGTCAGCATTACAGTCATGTGGATTCGAGCATGGAAGTCAGAAGTACAACGCAGATCTTGTTTGGCACGATTCCAACTACAGTCATTACCACTACGACGACTGCAGGCAGGAGAAGCATGGGATTTGATGGTGGCAAGTCGGTTGCTGATGGTAATTTGGACACTTAGTTCTCGCTGGTGGCGTATCACTCTAGTCTGGAGTCAAAGCACAATCCTTAGAGGCGTGGCTAG

mRNA sequence

ATGGGCCCTGGTGTTACGAAGGTTTCAGATAAAACTTCCCATCCATCACGATTAACAAGAACTCAAATGTCACTAAAGTTCTTGTCTTGTAATCAGTGGGTTCACCAGCTTCACCTTGAAAGCCCGGTGTCATTAACTACCGCCAAAGGAAGTCGGATAGAAGTTGGACCTAGATTTCTTAGACTACCACAAAACACTCAACATAAGAGCCTGTCTACAAGTTTATGCTTTGCTATGGATCAGAAAATCGTGCATGAGGGTGCCTTTGGTGTTAGTCAGAAAGATGGTGTGATTATCGTTGATCATGGATCTCGTCGGAAAGAGTCCAATCTCTTACTGAATGAATTTGTGCTGATGTTCAAAAGCAAAACTGGATACCCTATTGTTGAGCCGGCTCATATGGAATTGGCAGAACCATCCATAAAAGATGCTTTCAATTCATGTGTCGAGCAAGGGGCAAATCGTGTTATCATAAGCCCATTCTTCCTATTTCCTGGAAGGCATTGGCATCAGGATATTCCTTCCCTTGCAGATGCTGCTGCTCTGGAGCATCCCGGTATCAATTATCTTGTCACTGCACCGTTGGGTCTGCATGAGTTACTTGTGGATGTTGTGAAGGACAGAATTGACTATTGCTTAAGTCATGTAGCTGGAAAAGTGGAGGAATGTGCAGTCTGTGCTGGAACAGGCAGGTGTAAATTTAAAGTGCAGGATTCCGAAAATTAATGTGTAGTGATTTAAGCTACGCCGCACTTGTCAGTTTCATGGATCAAGCTAGATAGCATGTCTTTAGAACGTCAACTGTAATAACCGGAAGGTATGGCATTGCTATTTGGAGTACACATCACTTCCTGAGAAATATCTGCAGCATATGCCGTGGGTGCTGAATATCTGGATGTAGTGGCTTGTTTTCTCAGTGTATATGACAATCAATCTGAGTGGATCACGTGATAAATAAGTATTGTAACATCATCATGAATTCATGACAATGGTAATCTATGTACTGCATAAGACGGGGGGACTTAGATAGGTTGAGCAATGCAGAAGAGGAGGAAGGTCAGTCTTCCAGAAGTAAGAGTGCAGGTCAGCATTACAGTCATGTGGATTCGAGCATGGAAGTCAGAAGTACAACGCAGATCTTGTTTGGCACGATTCCAACTACAGTCATTACCACTACGACGACTGCAGGCAGGAGAAGCATGGGATTTGATGGTGGCAAGTCGGTTGCTGATGGTAATTTGGACACTTAGTTCTCGCTGGTGGCGTATCACTCTAGTCTGGAGTCAAAGCACAATCCTTAGAGGCGTGGCTAG

Coding sequence (CDS)

ATGGGCCCTGGTGTTACGAAGGTTTCAGATAAAACTTCCCATCCATCACGATTAACAAGAACTCAAATGTCACTAAAGTTCTTGTCTTGTAATCAGTGGGTTCACCAGCTTCACCTTGAAAGCCCGGTGTCATTAACTACCGCCAAAGGAAGTCGGATAGAAGTTGGACCTAGATTTCTTAGACTACCACAAAACACTCAACATAAGAGCCTGTCTACAAGTTTATGCTTTGCTATGGATCAGAAAATCGTGCATGAGGGTGCCTTTGGTGTTAGTCAGAAAGATGGTGTGATTATCGTTGATCATGGATCTCGTCGGAAAGAGTCCAATCTCTTACTGAATGAATTTGTGCTGATGTTCAAAAGCAAAACTGGATACCCTATTGTTGAGCCGGCTCATATGGAATTGGCAGAACCATCCATAAAAGATGCTTTCAATTCATGTGTCGAGCAAGGGGCAAATCGTGTTATCATAAGCCCATTCTTCCTATTTCCTGGAAGGCATTGGCATCAGGATATTCCTTCCCTTGCAGATGCTGCTGCTCTGGAGCATCCCGGTATCAATTATCTTGTCACTGCACCGTTGGGTCTGCATGAGTTACTTGTGGATGTTGTGAAGGACAGAATTGACTATTGCTTAAGTCATGTAGCTGGAAAAGTGGAGGAATGTGCAGTCTGTGCTGGAACAGGCAGGTGTAAATTTAAAGTGCAGGATTCCGAAAATTAA

Protein sequence

MGPGVTKVSDKTSHPSRLTRTQMSLKFLSCNQWVHQLHLESPVSLTTAKGSRIEVGPRFLRLPQNTQHKSLSTSLCFAMDQKIVHEGAFGVSQKDGVIIVDHGSRRKESNLLLNEFVLMFKSKTGYPIVEPAHMELAEPSIKDAFNSCVEQGANRVIISPFFLFPGRHWHQDIPSLADAAALEHPGINYLVTAPLGLHELLVDVVKDRIDYCLSHVAGKVEECAVCAGTGRCKFKVQDSEN

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo22007	Spo22007	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo22007.1	Spo22007.1-protein	polypeptide

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo22007.1.exon.1	Spo22007.1.exon.1	exon
Spo22007.1.exon.2	Spo22007.1.exon.2	exon
Spo22007.1.exon.3	Spo22007.1.exon.3	exon
Spo22007.1.exon.4	Spo22007.1.exon.4	exon
Spo22007.1.exon.5	Spo22007.1.exon.5	exon
Spo22007.1.exon.6	Spo22007.1.exon.6	exon
Spo22007.1.exon.7	Spo22007.1.exon.7	exon
Spo22007.1.exon.8	Spo22007.1.exon.8	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo22007.1.CDS.1	Spo22007.1.CDS.1	CDS
Spo22007.1.CDS.2	Spo22007.1.CDS.2	CDS
Spo22007.1.CDS.3	Spo22007.1.CDS.3	CDS
Spo22007.1.CDS.4	Spo22007.1.CDS.4	CDS
Spo22007.1.CDS.5	Spo22007.1.CDS.5	CDS
Spo22007.1.CDS.6	Spo22007.1.CDS.6	CDS
Spo22007.1.CDS.7	Spo22007.1.CDS.7	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo22007.1.utr3p.1	Spo22007.1.utr3p.1	three_prime_UTR
Spo22007.1.utr3p.2	Spo22007.1.utr3p.2	three_prime_UTR

Homology

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BLAST of Spo22007.1 vs. NCBI nr
Match: gi|902236265|gb|KNA24303.1| (hypothetical protein SOVF_017090 [Spinacia oleracea])

HSP 1 Score: 454.5 bits (1168), Expect = 1.100e-124
Identity = 219/219 (100.00%), Postives = 219/219 (100.00%), Query Frame = 1

		  
Query: 23  MSLKFLSCNQWVHQLHLESPVSLTTAKGSRIEVGPRFLRLPQNTQHKSLSTSLCFAMDQK 82
           MSLKFLSCNQWVHQLHLESPVSLTTAKGSRIEVGPRFLRLPQNTQHKSLSTSLCFAMDQK
Sbjct: 1   MSLKFLSCNQWVHQLHLESPVSLTTAKGSRIEVGPRFLRLPQNTQHKSLSTSLCFAMDQK 60

Query: 83  IVHEGAFGVSQKDGVIIVDHGSRRKESNLLLNEFVLMFKSKTGYPIVEPAHMELAEPSIK 142
           IVHEGAFGVSQKDGVIIVDHGSRRKESNLLLNEFVLMFKSKTGYPIVEPAHMELAEPSIK
Sbjct: 61  IVHEGAFGVSQKDGVIIVDHGSRRKESNLLLNEFVLMFKSKTGYPIVEPAHMELAEPSIK 120

Query: 143 DAFNSCVEQGANRVIISPFFLFPGRHWHQDIPSLADAAALEHPGINYLVTAPLGLHELLV 202
           DAFNSCVEQGANRVIISPFFLFPGRHWHQDIPSLADAAALEHPGINYLVTAPLGLHELLV
Sbjct: 121 DAFNSCVEQGANRVIISPFFLFPGRHWHQDIPSLADAAALEHPGINYLVTAPLGLHELLV 180

Query: 203 DVVKDRIDYCLSHVAGKVEECAVCAGTGRCKFKVQDSEN 242
           DVVKDRIDYCLSHVAGKVEECAVCAGTGRCKFKVQDSEN
Sbjct: 181 DVVKDRIDYCLSHVAGKVEECAVCAGTGRCKFKVQDSEN 219

[Close]

BLAST of Spo22007.1 vs. NCBI nr
Match: gi|902207097|gb|KNA15620.1| (hypothetical protein SOVF_096610 [Spinacia oleracea])

HSP 1 Score: 408.3 bits (1048), Expect = 9.300e-111
Identity = 195/219 (89.04%), Postives = 203/219 (92.69%), Query Frame = 1

		  
Query: 23  MSLKFLSCNQWVHQLHLESPVSLTTAKGSRIEVGPRFLRLPQNTQHKSLSTSLCFAMDQK 82
           MSLKFLSCNQWVHQLHL+SPVS TT K S IE GPR+ +LPQ+T  KSLS SLCFAMDQK
Sbjct: 1   MSLKFLSCNQWVHQLHLQSPVSSTTTKRSWIEAGPRYFKLPQSTLRKSLSPSLCFAMDQK 60

Query: 83  IVHEGAFGVSQKDGVIIVDHGSRRKESNLLLNEFVLMFKSKTGYPIVEPAHMELAEPSIK 142
            +HE AFGV QKDGVIIVDHGSRRKESNLLLNEFVLMFKSKTGYPIVEPAHMELA+PSIK
Sbjct: 61  SLHEDAFGVGQKDGVIIVDHGSRRKESNLLLNEFVLMFKSKTGYPIVEPAHMELADPSIK 120

Query: 143 DAFNSCVEQGANRVIISPFFLFPGRHWHQDIPSLADAAALEHPGINYLVTAPLGLHELLV 202
           DAFNSCV+QGANRVIISPFFLFPGRHWHQDIPSLADAAALEHPGINYLVTAPLGLHE LV
Sbjct: 121 DAFNSCVQQGANRVIISPFFLFPGRHWHQDIPSLADAAALEHPGINYLVTAPLGLHEFLV 180

Query: 203 DVVKDRIDYCLSHVAGKVEECAVCAGTGRCKFKVQDSEN 242
           DVVKDRI YCLSHVAG+VEECAVC GTGRCK KVQDSEN
Sbjct: 181 DVVKDRIGYCLSHVAGEVEECAVCVGTGRCKLKVQDSEN 219

[Close]

BLAST of Spo22007.1 vs. NCBI nr
Match: gi|731320521|ref|XP_010670852.1| (PREDICTED: uncharacterized protein LOC104887804 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 379.4 bits (973), Expect = 4.600e-102
Identity = 187/219 (85.39%), Postives = 197/219 (89.95%), Query Frame = 1

		  
Query: 23  MSLKFLSCNQWVHQLHLESPVS-LTTAKGSRIEVGPRFLRLPQNTQHKSLSTSLCFAMDQ 82
           MSLK LSCNQWVH   L+SPVS L TAKGSR EV PR+   P++ Q K LS SLCF MDQ
Sbjct: 1   MSLKLLSCNQWVH---LQSPVSFLPTAKGSRTEVAPRYFNPPKSIQLKRLSPSLCFPMDQ 60

Query: 83  KIVHEGAFGVSQKDGVIIVDHGSRRKESNLLLNEFVLMFKSKTGYPIVEPAHMELAEPSI 142
           K VHEGAFGV QKDGVIIVDHGSRRKESN+LLNEFVLMFKS+TGYPIVEPAHMELAEPSI
Sbjct: 61  KSVHEGAFGVGQKDGVIIVDHGSRRKESNILLNEFVLMFKSRTGYPIVEPAHMELAEPSI 120

Query: 143 KDAFNSCVEQGANRVIISPFFLFPGRHWHQDIPSLADAAALEHPGINYLVTAPLGLHELL 202
           KDAF+ CV+QGAN VIISPFFLFPGRHWHQDIP LA AAALEHPGINYLVTAPLGLHELL
Sbjct: 121 KDAFDLCVQQGANCVIISPFFLFPGRHWHQDIPVLAGAAALEHPGINYLVTAPLGLHELL 180

Query: 203 VDVVKDRIDYCLSHVAGKVEECAVCAGTGRCKFKVQDSE 241
           VDVVKDRIDYCLSHVAG+VEECAVCAGTGRCKFKVQ+SE
Sbjct: 181 VDVVKDRIDYCLSHVAGQVEECAVCAGTGRCKFKVQNSE 216

[Close]

BLAST of Spo22007.1 vs. NCBI nr
Match: gi|1021044711|gb|KZN02492.1| (hypothetical protein DCAR_011246 [Daucus carota subsp. sativus])

HSP 1 Score: 273.5 bits (698), Expect = 3.600e-70
Identity = 125/177 (70.62%), Postives = 149/177 (84.18%), Query Frame = 1

		  
Query: 57  PRFLRLPQNTQHKSLSTSLCFAMDQKIVHEGAFGVSQKDGVIIVDHGSRRKESNLLLNEF 116
           PRF++L  N++ +++ T LCFA       +   G+  KDGVIIVDHGSRRKESNL+LNEF
Sbjct: 30  PRFVKLSNNSK-RNVPTRLCFASSNGGFRQETSGIGSKDGVIIVDHGSRRKESNLMLNEF 89

Query: 117 VLMFKSKTGYPIVEPAHMELAEPSIKDAFNSCVEQGANRVIISPFFLFPGRHWHQDIPSL 176
           V+MF+ +TGYPIVEPAHMELAEPSIKDAF SCV+QG NRVI+SPFFLFPGRHW+QDIPSL
Sbjct: 90  VIMFRDRTGYPIVEPAHMELAEPSIKDAFASCVQQGVNRVIVSPFFLFPGRHWNQDIPSL 149

Query: 177 ADAAALEHPGINYLVTAPLGLHELLVDVVKDRIDYCLSHVAGKVEECAVCAGTGRCK 234
              AA EHPG++Y++TAPLGLHELLVDVV DRI +CLSHVAG  +EC+VCAGTGRC+
Sbjct: 150 TAEAAKEHPGVSYIITAPLGLHELLVDVVNDRIQHCLSHVAGDADECSVCAGTGRCQ 205

[Close]

BLAST of Spo22007.1 vs. NCBI nr
Match: gi|802542391|ref|XP_012081294.1| (PREDICTED: uncharacterized protein LOC105641377 [Jatropha curcas])

HSP 1 Score: 269.6 bits (688), Expect = 5.200e-69
Identity = 136/228 (59.65%), Postives = 162/228 (71.05%), Query Frame = 1

		  
Query: 10  DKTSHPSRLTRT---QMSLKFLSCNQWVHQLHLESPVSLTTAKGSRIEVGPRFLRLPQ-N 69
           D+T+  S+L      +M+LK L C             S+     S I   P+F +LP   
Sbjct: 8   DETTQSSQLDAVWLQKMALKPLLCPPQT----TSKSFSVNETGASPIWTPPKFSKLPGVP 67

Query: 70  TQHKSLSTSLCFAMDQKIVHEGAFGVSQKDGVIIVDHGSRRKESNLLLNEFVLMFKSKTG 129
            + K  ST LC       + + A GV ++DG+IIVDHGSRRKESNL+LNEFV MF+ K+G
Sbjct: 68  CKTKYSSTRLCLRTGNGEITKNAHGVRERDGLIIVDHGSRRKESNLMLNEFVAMFRDKSG 127

Query: 130 YPIVEPAHMELAEPSIKDAFNSCVEQGANRVIISPFFLFPGRHWHQDIPSLADAAALEHP 189
           YPIVEPAHMELAEPSIKDAF  CV+QGANRVI+SPFFLFPGRHWHQDIPSLA  AA EHP
Sbjct: 128 YPIVEPAHMELAEPSIKDAFGLCVQQGANRVIVSPFFLFPGRHWHQDIPSLAAEAAKEHP 187

Query: 190 GINYLVTAPLGLHELLVDVVKDRIDYCLSHVAGKVEECAVCAGTGRCK 234
           G++Y++TAPLGLHELLVDVV DRI +CL HVAG  +ECA C GTG+CK
Sbjct: 188 GVSYIITAPLGLHELLVDVVNDRIKHCLRHVAGDADECAACVGTGKCK 231

[Close]

BLAST of Spo22007.1 vs. UniProtKB/TrEMBL
Match: A0A0K9RXW9_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_017090 PE=4 SV=1)

HSP 1 Score: 454.5 bits (1168), Expect = 7.900e-125
Identity = 219/219 (100.00%), Postives = 219/219 (100.00%), Query Frame = 1

		  
Query: 23  MSLKFLSCNQWVHQLHLESPVSLTTAKGSRIEVGPRFLRLPQNTQHKSLSTSLCFAMDQK 82
           MSLKFLSCNQWVHQLHLESPVSLTTAKGSRIEVGPRFLRLPQNTQHKSLSTSLCFAMDQK
Sbjct: 1   MSLKFLSCNQWVHQLHLESPVSLTTAKGSRIEVGPRFLRLPQNTQHKSLSTSLCFAMDQK 60

Query: 83  IVHEGAFGVSQKDGVIIVDHGSRRKESNLLLNEFVLMFKSKTGYPIVEPAHMELAEPSIK 142
           IVHEGAFGVSQKDGVIIVDHGSRRKESNLLLNEFVLMFKSKTGYPIVEPAHMELAEPSIK
Sbjct: 61  IVHEGAFGVSQKDGVIIVDHGSRRKESNLLLNEFVLMFKSKTGYPIVEPAHMELAEPSIK 120

Query: 143 DAFNSCVEQGANRVIISPFFLFPGRHWHQDIPSLADAAALEHPGINYLVTAPLGLHELLV 202
           DAFNSCVEQGANRVIISPFFLFPGRHWHQDIPSLADAAALEHPGINYLVTAPLGLHELLV
Sbjct: 121 DAFNSCVEQGANRVIISPFFLFPGRHWHQDIPSLADAAALEHPGINYLVTAPLGLHELLV 180

Query: 203 DVVKDRIDYCLSHVAGKVEECAVCAGTGRCKFKVQDSEN 242
           DVVKDRIDYCLSHVAGKVEECAVCAGTGRCKFKVQDSEN
Sbjct: 181 DVVKDRIDYCLSHVAGKVEECAVCAGTGRCKFKVQDSEN 219

[Close]

BLAST of Spo22007.1 vs. UniProtKB/TrEMBL
Match: A0A0K9R808_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_096610 PE=4 SV=1)

HSP 1 Score: 408.3 bits (1048), Expect = 6.500e-111
Identity = 195/219 (89.04%), Postives = 203/219 (92.69%), Query Frame = 1

		  
Query: 23  MSLKFLSCNQWVHQLHLESPVSLTTAKGSRIEVGPRFLRLPQNTQHKSLSTSLCFAMDQK 82
           MSLKFLSCNQWVHQLHL+SPVS TT K S IE GPR+ +LPQ+T  KSLS SLCFAMDQK
Sbjct: 1   MSLKFLSCNQWVHQLHLQSPVSSTTTKRSWIEAGPRYFKLPQSTLRKSLSPSLCFAMDQK 60

Query: 83  IVHEGAFGVSQKDGVIIVDHGSRRKESNLLLNEFVLMFKSKTGYPIVEPAHMELAEPSIK 142
            +HE AFGV QKDGVIIVDHGSRRKESNLLLNEFVLMFKSKTGYPIVEPAHMELA+PSIK
Sbjct: 61  SLHEDAFGVGQKDGVIIVDHGSRRKESNLLLNEFVLMFKSKTGYPIVEPAHMELADPSIK 120

Query: 143 DAFNSCVEQGANRVIISPFFLFPGRHWHQDIPSLADAAALEHPGINYLVTAPLGLHELLV 202
           DAFNSCV+QGANRVIISPFFLFPGRHWHQDIPSLADAAALEHPGINYLVTAPLGLHE LV
Sbjct: 121 DAFNSCVQQGANRVIISPFFLFPGRHWHQDIPSLADAAALEHPGINYLVTAPLGLHEFLV 180

Query: 203 DVVKDRIDYCLSHVAGKVEECAVCAGTGRCKFKVQDSEN 242
           DVVKDRI YCLSHVAG+VEECAVC GTGRCK KVQDSEN
Sbjct: 181 DVVKDRIGYCLSHVAGEVEECAVCVGTGRCKLKVQDSEN 219

[Close]

BLAST of Spo22007.1 vs. UniProtKB/TrEMBL
Match: A0A0J8FMA1_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_2g044390 PE=4 SV=1)

HSP 1 Score: 379.4 bits (973), Expect = 3.200e-102
Identity = 187/219 (85.39%), Postives = 197/219 (89.95%), Query Frame = 1

		  
Query: 23  MSLKFLSCNQWVHQLHLESPVS-LTTAKGSRIEVGPRFLRLPQNTQHKSLSTSLCFAMDQ 82
           MSLK LSCNQWVH   L+SPVS L TAKGSR EV PR+   P++ Q K LS SLCF MDQ
Sbjct: 1   MSLKLLSCNQWVH---LQSPVSFLPTAKGSRTEVAPRYFNPPKSIQLKRLSPSLCFPMDQ 60

Query: 83  KIVHEGAFGVSQKDGVIIVDHGSRRKESNLLLNEFVLMFKSKTGYPIVEPAHMELAEPSI 142
           K VHEGAFGV QKDGVIIVDHGSRRKESN+LLNEFVLMFKS+TGYPIVEPAHMELAEPSI
Sbjct: 61  KSVHEGAFGVGQKDGVIIVDHGSRRKESNILLNEFVLMFKSRTGYPIVEPAHMELAEPSI 120

Query: 143 KDAFNSCVEQGANRVIISPFFLFPGRHWHQDIPSLADAAALEHPGINYLVTAPLGLHELL 202
           KDAF+ CV+QGAN VIISPFFLFPGRHWHQDIP LA AAALEHPGINYLVTAPLGLHELL
Sbjct: 121 KDAFDLCVQQGANCVIISPFFLFPGRHWHQDIPVLAGAAALEHPGINYLVTAPLGLHELL 180

Query: 203 VDVVKDRIDYCLSHVAGKVEECAVCAGTGRCKFKVQDSE 241
           VDVVKDRIDYCLSHVAG+VEECAVCAGTGRCKFKVQ+SE
Sbjct: 181 VDVVKDRIDYCLSHVAGQVEECAVCAGTGRCKFKVQNSE 216

[Close]

BLAST of Spo22007.1 vs. UniProtKB/TrEMBL
Match: A0A166B8C6_DAUCA (Uncharacterized protein OS=Daucus carota subsp. sativus GN=DCAR_011246 PE=4 SV=1)

HSP 1 Score: 273.5 bits (698), Expect = 2.500e-70
Identity = 125/177 (70.62%), Postives = 149/177 (84.18%), Query Frame = 1

		  
Query: 57  PRFLRLPQNTQHKSLSTSLCFAMDQKIVHEGAFGVSQKDGVIIVDHGSRRKESNLLLNEF 116
           PRF++L  N++ +++ T LCFA       +   G+  KDGVIIVDHGSRRKESNL+LNEF
Sbjct: 30  PRFVKLSNNSK-RNVPTRLCFASSNGGFRQETSGIGSKDGVIIVDHGSRRKESNLMLNEF 89

Query: 117 VLMFKSKTGYPIVEPAHMELAEPSIKDAFNSCVEQGANRVIISPFFLFPGRHWHQDIPSL 176
           V+MF+ +TGYPIVEPAHMELAEPSIKDAF SCV+QG NRVI+SPFFLFPGRHW+QDIPSL
Sbjct: 90  VIMFRDRTGYPIVEPAHMELAEPSIKDAFASCVQQGVNRVIVSPFFLFPGRHWNQDIPSL 149

Query: 177 ADAAALEHPGINYLVTAPLGLHELLVDVVKDRIDYCLSHVAGKVEECAVCAGTGRCK 234
              AA EHPG++Y++TAPLGLHELLVDVV DRI +CLSHVAG  +EC+VCAGTGRC+
Sbjct: 150 TAEAAKEHPGVSYIITAPLGLHELLVDVVNDRIQHCLSHVAGDADECSVCAGTGRCQ 205

[Close]

BLAST of Spo22007.1 vs. UniProtKB/TrEMBL
Match: A0A067LAA8_JATCU (Uncharacterized protein OS=Jatropha curcas GN=JCGZ_09571 PE=4 SV=1)

HSP 1 Score: 267.7 bits (683), Expect = 1.400e-68
Identity = 127/191 (66.49%), Postives = 148/191 (77.49%), Query Frame = 1

		  
Query: 44  SLTTAKGSRIEVGPRFLRLPQ-NTQHKSLSTSLCFAMDQKIVHEGAFGVSQKDGVIIVDH 103
           S+     S I   P+F +LP    + K  ST LC       + + A GV ++DG+IIVDH
Sbjct: 18  SVNETGASPIWTPPKFSKLPGVPCKTKYSSTRLCLRTGNGEITKNAHGVRERDGLIIVDH 77

Query: 104 GSRRKESNLLLNEFVLMFKSKTGYPIVEPAHMELAEPSIKDAFNSCVEQGANRVIISPFF 163
           GSRRKESNL+LNEFV MF+ K+GYPIVEPAHMELAEPSIKDAF  CV+QGANRVI+SPFF
Sbjct: 78  GSRRKESNLMLNEFVAMFRDKSGYPIVEPAHMELAEPSIKDAFGLCVQQGANRVIVSPFF 137

Query: 164 LFPGRHWHQDIPSLADAAALEHPGINYLVTAPLGLHELLVDVVKDRIDYCLSHVAGKVEE 223
           LFPGRHWHQDIPSLA  AA EHPG++Y++TAPLGLHELLVDVV DRI +CL HVAG  +E
Sbjct: 138 LFPGRHWHQDIPSLAAEAAKEHPGVSYIITAPLGLHELLVDVVNDRIKHCLRHVAGDADE 197

Query: 224 CAVCAGTGRCK 234
           CA C GTG+CK
Sbjct: 198 CAACVGTGKCK 208

[Close]

BLAST of Spo22007.1 vs. ExPASy Swiss-Prot
Match: SIRB_ARATH (Sirohydrochlorin ferrochelatase, chloroplastic OS=Arabidopsis thaliana GN=SIRB PE=1 SV=1)

HSP 1 Score: 236.1 bits (601), Expect = 4.000e-61
Identity = 110/160 (68.75%), Postives = 126/160 (78.75%), Query Frame = 1

		  
Query: 74  SLCFAMDQKIVHEGAFGVSQKDGVIIVDHGSRRKESNLLLNEFVLMFKSKTGYPIVEPAH 133
           S CF   + +V  G   +   DG+IIVDHGSRR+ESNL+L EFV MFK KTGYPIVEPAH
Sbjct: 64  SPCFGESEGLVKNG---IGDADGIIIVDHGSRRRESNLMLEEFVKMFKEKTGYPIVEPAH 123

Query: 134 MELAEPSIKDAFNSCVEQGANRVIISPFFLFPGRHWHQDIPSLADAAALEHPGINYLVTA 193
           MELAEPSIKDAF+ CV+QGA RV++SPFFLFPGRHWH DIPSL   AA E  GI+YL+TA
Sbjct: 124 MELAEPSIKDAFSLCVQQGAKRVVVSPFFLFPGRHWHTDIPSLTADAAKEFSGISYLITA 183

Query: 194 PLGLHELLVDVVKDRIDYCLSHVAGKVEECAVCAGTGRCK 234
           PLG H LL+DVV DRI +CLSHV G  +EC VCAGT +CK
Sbjct: 184 PLGPHNLLLDVVNDRIQHCLSHVEGDADECLVCAGTNKCK 220

[Close]

BLAST of Spo22007.1 vs. ExPASy Swiss-Prot
Match: SIRB_BACME (Sirohydrochlorin ferrochelatase OS=Bacillus megaterium GN=sirB PE=3 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 2.500e-15
Identity = 46/132 (34.85%), Postives = 64/132 (48.48%), Query Frame = 1

		  
Query: 79  MDQKIVHEGAFGVSQKDGVIIVDHGSRRKESNLLLNEFVLMFKSKTGYPIVEPAHMELAE 138
           M +K+  E    V   D V+ V HGSR KE       F+   K     PI E   +ELA 
Sbjct: 1   MHKKLTKE----VDYMDAVLYVCHGSRVKEGADQAVAFIERCKKNLDVPIQEVCFLELAS 60

Query: 139 PSIKDAFNSCVEQGANRVIISPFFLFPGRHWHQDIPSLADAAALEHPGINYLVTAPLGLH 198
           P+I+  F +C+EQGA R+ I P  L    H   DIP         +P +  L   P G+ 
Sbjct: 61  PTIEQGFEACIEQGATRIAIVPLLLLTAAHAKHDIPEEIQKVYERYPQVEVLYGEPFGVD 120

Query: 199 ELLVDVVKDRID 211
           E +VD++ +RI+
Sbjct: 121 ERIVDILVERIN 128

[Close]

BLAST of Spo22007.1 vs. ExPASy Swiss-Prot
Match: CBIX_BACME (Sirohydrochlorin cobaltochelatase OS=Bacillus megaterium GN=cbiX PE=1 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 9.000e-13
Identity = 40/130 (30.77%), Postives = 66/130 (50.77%), Query Frame = 1

		  
Query: 97  VIIVDHGSRRKESNLLLNEFVLMFKSKTGYPI-VEPAHMELAEPSIKDAFNSCVEQGANR 156
           V+ V HGSR  E N    EF+   K      I VE   +E   P++    ++CV +GA  
Sbjct: 9   VLFVGHGSRDPEGND--REFISTMKHDWDASILVETCFLEFERPNVSQGIDTCVAKGAQD 68

Query: 157 VIISPFFLFPGRHWHQDIPSLADAAALEHPGINYLVTAPLGLHELLVDVVKDRIDYCLSH 216
           V++ P  L P  H    IP+  D A  ++P +N++   P+G+HE  ++++K R+     +
Sbjct: 69  VVVIPIMLLPAGHSKIHIPAAIDEAKEKYPHVNFVYGRPIGVHEEALEILKTRLQESGEN 128

Query: 217 VAGKVEECAV 226
           +    E+ AV
Sbjct: 129 LETPAEDTAV 136

[Close]

BLAST of Spo22007.1 vs. ExPASy Swiss-Prot
Match: CBIX_METMP (Sirohydrochlorin cobaltochelatase OS=Methanococcus maripaludis (strain S2 / LL) GN=cbiX PE=3 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 1.200e-12
Identity = 41/139 (29.50%), Postives = 65/139 (46.76%), Query Frame = 1

		  
Query: 95  DGVIIVDHGSRRKESNLLLNEFVLMFKSKTGYPIVEPAHMELAEPSIKDAFNSCVEQGAN 154
           + +++V HGSR   S  ++ E     K++  Y IVE   ME  EP+I       ++ GA 
Sbjct: 2   EALVLVGHGSRLPHSKNVVMEVAEKIKARNIYDIVEVGMMEFNEPTIPQTIKKVIDAGAK 61

Query: 155 RVIISPFFLFPGRHWHQDIPSLA------------------------DAAALEHP-GINY 209
           +VI++P FL PG H  +DIP +                         D  A+E P G+  
Sbjct: 62  KVIVTPVFLAPGNHTERDIPKILGIYEGDDEGGHHHHHDHEHHHHHHDTTAVEIPEGVEL 121

[Close]

BLAST of Spo22007.1 vs. ExPASy Swiss-Prot
Match: SIRB_BACSU (Sirohydrochlorin ferrochelatase OS=Bacillus subtilis (strain 168) GN=sirB PE=2 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 7.600e-12
Identity = 36/116 (31.03%), Postives = 57/116 (49.14%), Query Frame = 1

		  
Query: 94  KDGVIIVDHGSRRKESNLLLNEFVLMFKSKTGYPIVEPAHMELAEPSIKDAFNSCVEQGA 153
           K  ++ V HGSR K++      F+   K+    P+ E + +EL EP+I+  F +CV+QGA
Sbjct: 2   KQAILYVGHGSRVKKAQQEAAAFLEGCKAHISVPVQEISFLELQEPTIETGFEACVKQGA 61

Query: 154 NRVIISPFFLFPGRHWHQDIPSLADAAALEHPGINYLVTAPLGLHELLVDVVKDRI 210
             + + P  L    H   DIP      A  +P +      P+G+ E +V  V  R+
Sbjct: 62  THIAVVPLLLLTAAHAKHDIPEEIVRVASRYPSVRISYGKPIGIDEEVVKAVYHRM 117

[Close]

BLAST of Spo22007.1 vs. TAIR (Arabidopsis)
Match: AT1G50170.1 (sirohydrochlorin ferrochelatase B)

HSP 1 Score: 236.1 bits (601), Expect = 2.200e-62
Identity = 110/160 (68.75%), Postives = 126/160 (78.75%), Query Frame = 1

		  
Query: 74  SLCFAMDQKIVHEGAFGVSQKDGVIIVDHGSRRKESNLLLNEFVLMFKSKTGYPIVEPAH 133
           S CF   + +V  G   +   DG+IIVDHGSRR+ESNL+L EFV MFK KTGYPIVEPAH
Sbjct: 64  SPCFGESEGLVKNG---IGDADGIIIVDHGSRRRESNLMLEEFVKMFKEKTGYPIVEPAH 123

Query: 134 MELAEPSIKDAFNSCVEQGANRVIISPFFLFPGRHWHQDIPSLADAAALEHPGINYLVTA 193
           MELAEPSIKDAF+ CV+QGA RV++SPFFLFPGRHWH DIPSL   AA E  GI+YL+TA
Sbjct: 124 MELAEPSIKDAFSLCVQQGAKRVVVSPFFLFPGRHWHTDIPSLTADAAKEFSGISYLITA 183

Query: 194 PLGLHELLVDVVKDRIDYCLSHVAGKVEECAVCAGTGRCK 234
           PLG H LL+DVV DRI +CLSHV G  +EC VCAGT +CK
Sbjct: 184 PLGPHNLLLDVVNDRIQHCLSHVEGDADECLVCAGTNKCK 220

The following BLAST results are available for this feature:

BLAST of Spo22007.1 vs. NCBI nr
BLAST of Spo22007.1 vs. UniProtKB/TrEMBL
BLAST of Spo22007.1 vs. ExPASy Swiss-Prot
BLAST of Spo22007.1 vs. TAIR (Arabidopsis)

BLAST of Spo22007.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5

Position :

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Match Name	E-value	Identity	Description
gi\|902236265\|gb\|KNA24303.1\|	1.1e-124	100.	hypothetical protein SOVF_0170...	[more]
gi\|902207097\|gb\|KNA15620.1\|	9.3e-111	89.0	hypothetical protein SOVF_0966...	[more]
gi\|731320521\|ref\|XP_010670852.1\|	4.6e-102	85.3	PREDICTED: uncharacterized pro...	[more]
gi\|1021044711\|gb\|KZN02492.1\|	3.6e-70	70.6	hypothetical protein DCAR_0112...	[more]
gi\|802542391\|ref\|XP_012081294.1\|	5.2e-69	59.6	PREDICTED: uncharacterized pro...	[more]

BLAST of Spo22007.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5

Position :

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Match Name	E-value	Identity	Description
A0A0K9RXW9_SPIOL	7.9e-125	100.	Uncharacterized protein OS=Spi...	[more]
A0A0K9R808_SPIOL	6.5e-111	89.0	Uncharacterized protein OS=Spi...	[more]
A0A0J8FMA1_BETVU	3.2e-102	85.3	Uncharacterized protein OS=Bet...	[more]
A0A166B8C6_DAUCA	2.5e-70	70.6	Uncharacterized protein OS=Dau...	[more]
A0A067LAA8_JATCU	1.4e-68	66.4	Uncharacterized protein OS=Jat...	[more]

BLAST of Spo22007.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 5

Position :

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Match Name	E-value	Identity	Description
SIRB_ARATH	4.0e-61	68.7	Sirohydrochlorin ferrochelatas...	[more]
SIRB_BACME	2.5e-15	34.8	Sirohydrochlorin ferrochelatas...	[more]
CBIX_BACME	9.0e-13	30.7	Sirohydrochlorin cobaltochelat...	[more]
CBIX_METMP	1.2e-12	29.5	Sirohydrochlorin cobaltochelat...	[more]
SIRB_BACSU	7.6e-12	31.0	Sirohydrochlorin ferrochelatas...	[more]

BLAST of Spo22007.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 1

Position :

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Match Name	E-value	Identity	Description
AT1G50170.1	2.2e-62	68.7	sirohydrochlorin ferrochelatas...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002762	Cobalamin (vitamin B12) biosynthesis CbiX	PFAM	PF01903	CbiX	coord: 102..206 score: 1.0
None	No IPR available	GENE3D	3.40.50.1400		coord: 93..215 score: 6.2
None	No IPR available	PANTHER	PTHR33542	FAMILY NOT NAMED	coord: 74..234 score: 3.2
None	No IPR available	unknown	SSF53800	Chelatase	coord: 94..210 score: 2.22

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009236	cobalamin biosynthetic process
biological_process	GO:0006979	response to oxidative stress
biological_process	GO:0019354	siroheme biosynthetic process
cellular_component	GO:0009507	chloroplast
molecular_function	GO:0016852	sirohydrochlorin cobaltochelatase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0051266	sirohydrochlorin ferrochelatase activity