Spo22017.1 (mRNA) | SpinachBase

Overview


Name	Spo22017.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	ATP-dependent protease La (LON) domain protein
Location	chr4 : 112559415 .. 112560275 (+)
Sequence length	861

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGCTCCCTCAATTCCTCCATTATTCCACCACCACCATAACCACCCCTAAACCCTCCTTAAACCCCACGCCACCCACTTTCCTCCACCCTTCCCCACTCTCTCTCCTCCACAGCCACCGTAAACACCACCGGAACCTCCGCTGCTCCGCCTCCTCATCCTCCCCACAACCCCACAACAATCCTCCTTCCCCTAACCCCACCCCACGAAACGACGTTGTAGAACTCCCCATCTTCCCCCTCCCTTTAGTCCTCTTCCCTGGCTCAATCCTCCCATTACAAATCTTCGAGTTTCGCTACCGTATAATGATGCATACCCTCCTCCAGACCGACCTCCGTTTCGGAGTTATTTTCTCCGATACCCTCTCCGCCGCTGCAACCTCCGTCGGTTGCGTCGGTGAGGTCGTCAAGCACGAGCGCCTCGCCGACGATCGCTTCTTCCTAATCTGCAAGGGTCAGGAACGCTTCCGTATCAATAAGATCGTACGGTCCAAACCTTACCTCGTGGCAGCCGTGGATTGGCTCGAGGATAAGCCCTCCTCCGATGGATCCCACGAGGATTTGGAGAGCTTGGCGAAGGATGTGGAGAGCTACATGAGGGATGTGATTAGGTTGAGCAATCGGTTGAATGGGAAGGAAGAGAAAGAGGTTGGGGATTTACGACGGAATTTATTTCCAACGCCGTTTTCGTTCTTTGTAGGGAGCACGTTCGAAGGTGCGCCGCGCGAGCAGCAGGCGTTGCTGGAGCTGGAGGATACCGCGGCGCGGTTGAGGAGAGAGAAGGAGACTTTGAGGAATACTTTGAATTATTTGAGTGCTGCTTCTGCTGTTAAGGATGTGTTTCCATCTTCTTCAACATAG

mRNA sequence

ATGGCGCTCCCTCAATTCCTCCATTATTCCACCACCACCATAACCACCCCTAAACCCTCCTTAAACCCCACGCCACCCACTTTCCTCCACCCTTCCCCACTCTCTCTCCTCCACAGCCACCGTAAACACCACCGGAACCTCCGCTGCTCCGCCTCCTCATCCTCCCCACAACCCCACAACAATCCTCCTTCCCCTAACCCCACCCCACGAAACGACGTTGTAGAACTCCCCATCTTCCCCCTCCCTTTAGTCCTCTTCCCTGGCTCAATCCTCCCATTACAAATCTTCGAGTTTCGCTACCGTATAATGATGCATACCCTCCTCCAGACCGACCTCCGTTTCGGAGTTATTTTCTCCGATACCCTCTCCGCCGCTGCAACCTCCGTCGGTTGCGTCGGTGAGGTCGTCAAGCACGAGCGCCTCGCCGACGATCGCTTCTTCCTAATCTGCAAGGGTCAGGAACGCTTCCGTATCAATAAGATCGTACGGTCCAAACCTTACCTCGTGGCAGCCGTGGATTGGCTCGAGGATAAGCCCTCCTCCGATGGATCCCACGAGGATTTGGAGAGCTTGGCGAAGGATGTGGAGAGCTACATGAGGGATGTGATTAGGTTGAGCAATCGGTTGAATGGGAAGGAAGAGAAAGAGGTTGGGGATTTACGACGGAATTTATTTCCAACGCCGTTTTCGTTCTTTGTAGGGAGCACGTTCGAAGGTGCGCCGCGCGAGCAGCAGGCGTTGCTGGAGCTGGAGGATACCGCGGCGCGGTTGAGGAGAGAGAAGGAGACTTTGAGGAATACTTTGAATTATTTGAGTGCTGCTTCTGCTGTTAAGGATGTGTTTCCATCTTCTTCAACATAG

Coding sequence (CDS)

ATGGCGCTCCCTCAATTCCTCCATTATTCCACCACCACCATAACCACCCCTAAACCCTCCTTAAACCCCACGCCACCCACTTTCCTCCACCCTTCCCCACTCTCTCTCCTCCACAGCCACCGTAAACACCACCGGAACCTCCGCTGCTCCGCCTCCTCATCCTCCCCACAACCCCACAACAATCCTCCTTCCCCTAACCCCACCCCACGAAACGACGTTGTAGAACTCCCCATCTTCCCCCTCCCTTTAGTCCTCTTCCCTGGCTCAATCCTCCCATTACAAATCTTCGAGTTTCGCTACCGTATAATGATGCATACCCTCCTCCAGACCGACCTCCGTTTCGGAGTTATTTTCTCCGATACCCTCTCCGCCGCTGCAACCTCCGTCGGTTGCGTCGGTGAGGTCGTCAAGCACGAGCGCCTCGCCGACGATCGCTTCTTCCTAATCTGCAAGGGTCAGGAACGCTTCCGTATCAATAAGATCGTACGGTCCAAACCTTACCTCGTGGCAGCCGTGGATTGGCTCGAGGATAAGCCCTCCTCCGATGGATCCCACGAGGATTTGGAGAGCTTGGCGAAGGATGTGGAGAGCTACATGAGGGATGTGATTAGGTTGAGCAATCGGTTGAATGGGAAGGAAGAGAAAGAGGTTGGGGATTTACGACGGAATTTATTTCCAACGCCGTTTTCGTTCTTTGTAGGGAGCACGTTCGAAGGTGCGCCGCGCGAGCAGCAGGCGTTGCTGGAGCTGGAGGATACCGCGGCGCGGTTGAGGAGAGAGAAGGAGACTTTGAGGAATACTTTGAATTATTTGAGTGCTGCTTCTGCTGTTAAGGATGTGTTTCCATCTTCTTCAACATAG

Protein sequence

MALPQFLHYSTTTITTPKPSLNPTPPTFLHPSPLSLLHSHRKHHRNLRCSASSSSPQPHNNPPSPNPTPRNDVVELPIFPLPLVLFPGSILPLQIFEFRYRIMMHTLLQTDLRFGVIFSDTLSAAATSVGCVGEVVKHERLADDRFFLICKGQERFRINKIVRSKPYLVAAVDWLEDKPSSDGSHEDLESLAKDVESYMRDVIRLSNRLNGKEEKEVGDLRRNLFPTPFSFFVGSTFEGAPREQQALLELEDTAARLRREKETLRNTLNYLSAASAVKDVFPSSST

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo22017	Spo22017	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo22017.1	Spo22017.1-protein	polypeptide

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo22017.1.exon.1	Spo22017.1.exon.1	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo22017.1.CDS.1	Spo22017.1.CDS.1	CDS

Homology

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BLAST of Spo22017.1 vs. NCBI nr
Match: gi|902141031|gb|KNA04675.1| (hypothetical protein SOVF_197470 [Spinacia oleracea])

HSP 1 Score: 562.4 bits (1448), Expect = 4.600e-157
Identity = 286/286 (100.00%), Postives = 286/286 (100.00%), Query Frame = 1


		  
Query: 1   MALPQFLHYSTTTITTPKPSLNPTPPTFLHPSPLSLLHSHRKHHRNLRCSASSSSPQPHN 60
           MALPQFLHYSTTTITTPKPSLNPTPPTFLHPSPLSLLHSHRKHHRNLRCSASSSSPQPHN
Sbjct: 1   MALPQFLHYSTTTITTPKPSLNPTPPTFLHPSPLSLLHSHRKHHRNLRCSASSSSPQPHN 60

Query: 61  NPPSPNPTPRNDVVELPIFPLPLVLFPGSILPLQIFEFRYRIMMHTLLQTDLRFGVIFSD 120
           NPPSPNPTPRNDVVELPIFPLPLVLFPGSILPLQIFEFRYRIMMHTLLQTDLRFGVIFSD
Sbjct: 61  NPPSPNPTPRNDVVELPIFPLPLVLFPGSILPLQIFEFRYRIMMHTLLQTDLRFGVIFSD 120

Query: 121 TLSAAATSVGCVGEVVKHERLADDRFFLICKGQERFRINKIVRSKPYLVAAVDWLEDKPS 180
           TLSAAATSVGCVGEVVKHERLADDRFFLICKGQERFRINKIVRSKPYLVAAVDWLEDKPS
Sbjct: 121 TLSAAATSVGCVGEVVKHERLADDRFFLICKGQERFRINKIVRSKPYLVAAVDWLEDKPS 180

Query: 181 SDGSHEDLESLAKDVESYMRDVIRLSNRLNGKEEKEVGDLRRNLFPTPFSFFVGSTFEGA 240
           SDGSHEDLESLAKDVESYMRDVIRLSNRLNGKEEKEVGDLRRNLFPTPFSFFVGSTFEGA
Sbjct: 181 SDGSHEDLESLAKDVESYMRDVIRLSNRLNGKEEKEVGDLRRNLFPTPFSFFVGSTFEGA 240

Query: 241 PREQQALLELEDTAARLRREKETLRNTLNYLSAASAVKDVFPSSST 287
           PREQQALLELEDTAARLRREKETLRNTLNYLSAASAVKDVFPSSST
Sbjct: 241 PREQQALLELEDTAARLRREKETLRNTLNYLSAASAVKDVFPSSST 286

[Close]

BLAST of Spo22017.1 vs. NCBI nr
Match: gi|731312083|ref|XP_010672533.1| (PREDICTED: uncharacterized protein LOC104889085 [Beta vulgaris subsp. vulgaris])

HSP 1 Score: 456.8 bits (1174), Expect = 2.700e-125
Identity = 241/287 (83.97%), Postives = 259/287 (90.24%), Query Frame = 1


		  
Query: 2   ALPQFLHYSTTTITTPKPSLNPTPPTFLHPSPLSLLHSHRKHHRNL--RCSASSSSPQPH 61
           +LPQFL  STTT+   KPSLNPTP      SPLSLL+  RK H +L  RCSASSSS  P 
Sbjct: 3   SLPQFLPSSTTTL---KPSLNPTPT-----SPLSLLNLRRKTHLHLHLRCSASSSSSHPQ 62

Query: 62  NNPPSPNPTPRNDVVELPIFPLPLVLFPGSILPLQIFEFRYRIMMHTLLQTDLRFGVIFS 121
           NN P  NP+P+ND+VELPIFPLPLVLFPGSILPLQIFEFRYRIMMHTLLQTDLRFG+IF+
Sbjct: 63  NNLPPSNPSPQNDIVELPIFPLPLVLFPGSILPLQIFEFRYRIMMHTLLQTDLRFGIIFT 122

Query: 122 DTLSAAATSVGCVGEVVKHERLADDRFFLICKGQERFRINKIVRSKPYLVAAVDWLEDKP 181
           D+LS+  TS+GCVGEVVKHE+L+DDRFFLICKGQERFRINKIVRSKPYLVAAVDWLEDKP
Sbjct: 123 DSLSSTVTSIGCVGEVVKHEKLSDDRFFLICKGQERFRINKIVRSKPYLVAAVDWLEDKP 182

Query: 182 SSDGSHEDLESLAKDVESYMRDVIRLSNRLNGKEEKEVGDLRRNLFPTPFSFFVGSTFEG 241
           S DGSHEDL++LAKDVE YMRDVIRLSNRLNGKEEKEV DLRRNLFPTPFSFFVGSTFEG
Sbjct: 183 SIDGSHEDLDALAKDVEGYMRDVIRLSNRLNGKEEKEVADLRRNLFPTPFSFFVGSTFEG 242

Query: 242 APREQQALLELEDTAARLRREKETLRNTLNYLSAASAVKDVFPSSST 287
           APREQQALLELEDTAARL+REKETLRNTLNYLSAASAVKDVFPSSS+
Sbjct: 243 APREQQALLELEDTAARLKREKETLRNTLNYLSAASAVKDVFPSSSS 281

[Close]

BLAST of Spo22017.1 vs. NCBI nr
Match: gi|565350052|ref|XP_006341988.1| (PREDICTED: lon protease 2-like [Solanum tuberosum])

HSP 1 Score: 380.6 bits (976), Expect = 2.500e-102
Identity = 213/293 (72.70%), Postives = 239/293 (81.57%), Query Frame = 1


		  
Query: 1   MALPQFLHYSTTTITTPKPSLNPTP----PTFLHP-----SPLSLLHSHRKHHRNLRCSA 60
           MALPQ L  S ++  T KP LNP+     P F +P     S L L H  +++   +RCSA
Sbjct: 1   MALPQILP-SPSSSFTHKPHLNPSNFFLIPQFNNPQCQFSSKLLLQHRRQRNSNGIRCSA 60

Query: 61  SSSSPQPHNNPPSPNPTPRNDVVELPIFPLPLVLFPGSILPLQIFEFRYRIMMHTLLQTD 120
           SSS P+ H+   SP     +DVVELP+FPLPLVLFPG+ILPLQIFEFRYRIMMHTLLQTD
Sbjct: 61  SSSFPEKHHTG-SPKS---DDVVELPLFPLPLVLFPGAILPLQIFEFRYRIMMHTLLQTD 120

Query: 121 LRFGVIFSDTLSAAATSVGCVGEVVKHERLADDRFFLICKGQERFRINKIVRSKPYLVAA 180
           LRFGVI+SD+ +  A  VGCVGEVVKHERL DDRFFLICKGQERFR+NK+VRSKPYLVA 
Sbjct: 121 LRFGVIYSDSATGTA-DVGCVGEVVKHERLVDDRFFLICKGQERFRVNKLVRSKPYLVAE 180

Query: 181 VDWLEDKPSSDGSHEDLESLAKDVESYMRDVIRLSNRLNGKEEKEVGDLRRNLFPTPFSF 240
           V WLED+PS++G  +D+E LA +VESYM+DVIRLSNRLNGK EKE  DLRRNLFPTPFSF
Sbjct: 181 VTWLEDRPSANGE-DDVEGLANEVESYMKDVIRLSNRLNGKPEKEATDLRRNLFPTPFSF 240

Query: 241 FVGSTFEGAPREQQALLELEDTAARLRREKETLRNTLNYLSAASAVKDVFPSS 285
           FVGSTFEGAPREQQALLELEDTA RL+REKETLRNTLNYLSAASAVKDVFPSS
Sbjct: 241 FVGSTFEGAPREQQALLELEDTAMRLKREKETLRNTLNYLSAASAVKDVFPSS 286

[Close]

BLAST of Spo22017.1 vs. NCBI nr
Match: gi|976912871|gb|KVH99323.1| (hypothetical protein Ccrd_022445 [Cynara cardunculus var. scolymus])

HSP 1 Score: 379.0 bits (972), Expect = 7.200e-102
Identity = 214/293 (73.04%), Postives = 239/293 (81.57%), Query Frame = 1


		  
Query: 1   MALPQFLHYSTTTITTPKPSLNPTPPTFLHPS---PLSLLHSHRKHHRN----LRCSASS 60
           MALPQ L   +++  T    LNP   +F  PS    +SL H  R+HHRN    LRCS+SS
Sbjct: 1   MALPQLLPSPSSSYLTHH-RLNPHTLSF-SPSNFNSISLPH-RRRHHRNHTSSLRCSSSS 60

Query: 61  SSPQPHNNPPSPNPTPRNDVVELPIFPLPLVLFPGSILPLQIFEFRYRIMMHTLLQTDLR 120
            S + H N PSPN    ND+VELP+FPLPLVLFPG+ILPLQIFE+RYRIMMHTLLQTDLR
Sbjct: 61  FS-EKHENTPSPNS---NDIVELPLFPLPLVLFPGAILPLQIFEYRYRIMMHTLLQTDLR 120

Query: 121 FGVIFSDTLSAAATSVGCVGEVVKHERLADDRFFLICKGQERFRINKIVRSKPYLVAAVD 180
           FGVI++D  S  A  VGCVGEVVKHERL DDRFF+ICKGQERFR+ K+VR+KPYLVA V 
Sbjct: 121 FGVIYTDATSGTA-DVGCVGEVVKHERLVDDRFFIICKGQERFRVTKVVRTKPYLVAEVV 180

Query: 181 WLEDKPSSDGSHEDLESLAKDVESYMRDVIRLSNRLNGKEEKEVGDLRRNLFPTPFSFFV 240
           WLED+PS +G  EDLE LA +VES+M+DVIRLSNRLNGK EKE GDLRRNLFPTPFSFFV
Sbjct: 181 WLEDRPSGNGE-EDLEGLASEVESHMKDVIRLSNRLNGKPEKEAGDLRRNLFPTPFSFFV 240

Query: 241 GSTFEGAPREQQALLELEDTAARLRREKETLRNTLNYLSAASAVKDVFPSSST 287
           GSTFEGAPREQQALLELEDT  RL+REKETLRNTLNYLSAASAVKDVFPSSS+
Sbjct: 241 GSTFEGAPREQQALLELEDTMVRLKREKETLRNTLNYLSAASAVKDVFPSSSS 284

[Close]

BLAST of Spo22017.1 vs. NCBI nr
Match: gi|698558454|ref|XP_009771292.1| (PREDICTED: uncharacterized protein LOC104221853 [Nicotiana sylvestris])

HSP 1 Score: 377.9 bits (969), Expect = 1.600e-101
Identity = 213/294 (72.45%), Postives = 239/294 (81.29%), Query Frame = 1


		  
Query: 1   MALPQFLHYSTTTITTPKPSL-NPTPPTFLHP--------SPLSLLHSHRKHHR-NLRCS 60
           MALPQ L  S++   T KP L NPT   FL P        +PL  LH  R+ +   LRCS
Sbjct: 1   MALPQILPSSSSF--THKPHLINPTNNFFLTPQSNPQYHFTPLPFLHRRRRRNNYTLRCS 60

Query: 61  ASSSSPQPHNNPPSPNPTPRNDVVELPIFPLPLVLFPGSILPLQIFEFRYRIMMHTLLQT 120
           ASS  P+ H+   SPN    +DVVELP+FPLPLVLFPG+ILPLQIFEFRYRIMMHTLLQT
Sbjct: 61  ASSF-PEKHHTGSSPNS---DDVVELPLFPLPLVLFPGAILPLQIFEFRYRIMMHTLLQT 120

Query: 121 DLRFGVIFSDTLSAAATSVGCVGEVVKHERLADDRFFLICKGQERFRINKIVRSKPYLVA 180
           DLRFGVI+SD+ +  A  VGCVGEVVKHERL DDRFFLICKGQERFR++K+VRSKPYLVA
Sbjct: 121 DLRFGVIYSDSATGTA-DVGCVGEVVKHERLVDDRFFLICKGQERFRVSKVVRSKPYLVA 180

Query: 181 AVDWLEDKPSSDGSHEDLESLAKDVESYMRDVIRLSNRLNGKEEKEVGDLRRNLFPTPFS 240
            V WLED+PS++G  +D+E LA +VE+YM+DVIRLSNRLNGK EKE  DLRRNLFPTPFS
Sbjct: 181 EVTWLEDRPSANGE-DDVEGLANEVENYMKDVIRLSNRLNGKAEKEATDLRRNLFPTPFS 240

Query: 241 FFVGSTFEGAPREQQALLELEDTAARLRREKETLRNTLNYLSAASAVKDVFPSS 285
           FFVGSTFEGAPREQQALLELEDTA RL+REKETLRNTLNYL+AASAVKDVFPSS
Sbjct: 241 FFVGSTFEGAPREQQALLELEDTAVRLKREKETLRNTLNYLTAASAVKDVFPSS 286

[Close]

BLAST of Spo22017.1 vs. UniProtKB/TrEMBL
Match: A0A0K9QBP3_SPIOL (Uncharacterized protein OS=Spinacia oleracea GN=SOVF_197470 PE=4 SV=1)

HSP 1 Score: 562.4 bits (1448), Expect = 3.200e-157
Identity = 286/286 (100.00%), Postives = 286/286 (100.00%), Query Frame = 1


		  
Query: 1   MALPQFLHYSTTTITTPKPSLNPTPPTFLHPSPLSLLHSHRKHHRNLRCSASSSSPQPHN 60
           MALPQFLHYSTTTITTPKPSLNPTPPTFLHPSPLSLLHSHRKHHRNLRCSASSSSPQPHN
Sbjct: 1   MALPQFLHYSTTTITTPKPSLNPTPPTFLHPSPLSLLHSHRKHHRNLRCSASSSSPQPHN 60

Query: 61  NPPSPNPTPRNDVVELPIFPLPLVLFPGSILPLQIFEFRYRIMMHTLLQTDLRFGVIFSD 120
           NPPSPNPTPRNDVVELPIFPLPLVLFPGSILPLQIFEFRYRIMMHTLLQTDLRFGVIFSD
Sbjct: 61  NPPSPNPTPRNDVVELPIFPLPLVLFPGSILPLQIFEFRYRIMMHTLLQTDLRFGVIFSD 120

Query: 121 TLSAAATSVGCVGEVVKHERLADDRFFLICKGQERFRINKIVRSKPYLVAAVDWLEDKPS 180
           TLSAAATSVGCVGEVVKHERLADDRFFLICKGQERFRINKIVRSKPYLVAAVDWLEDKPS
Sbjct: 121 TLSAAATSVGCVGEVVKHERLADDRFFLICKGQERFRINKIVRSKPYLVAAVDWLEDKPS 180

Query: 181 SDGSHEDLESLAKDVESYMRDVIRLSNRLNGKEEKEVGDLRRNLFPTPFSFFVGSTFEGA 240
           SDGSHEDLESLAKDVESYMRDVIRLSNRLNGKEEKEVGDLRRNLFPTPFSFFVGSTFEGA
Sbjct: 181 SDGSHEDLESLAKDVESYMRDVIRLSNRLNGKEEKEVGDLRRNLFPTPFSFFVGSTFEGA 240

Query: 241 PREQQALLELEDTAARLRREKETLRNTLNYLSAASAVKDVFPSSST 287
           PREQQALLELEDTAARLRREKETLRNTLNYLSAASAVKDVFPSSST
Sbjct: 241 PREQQALLELEDTAARLRREKETLRNTLNYLSAASAVKDVFPSSST 286

[Close]

BLAST of Spo22017.1 vs. UniProtKB/TrEMBL
Match: A0A0J8FYF5_BETVU (Uncharacterized protein OS=Beta vulgaris subsp. vulgaris GN=BVRB_1g006510 PE=4 SV=1)

HSP 1 Score: 456.8 bits (1174), Expect = 1.900e-125
Identity = 241/287 (83.97%), Postives = 259/287 (90.24%), Query Frame = 1


		  
Query: 2   ALPQFLHYSTTTITTPKPSLNPTPPTFLHPSPLSLLHSHRKHHRNL--RCSASSSSPQPH 61
           +LPQFL  STTT+   KPSLNPTP      SPLSLL+  RK H +L  RCSASSSS  P 
Sbjct: 3   SLPQFLPSSTTTL---KPSLNPTPT-----SPLSLLNLRRKTHLHLHLRCSASSSSSHPQ 62

Query: 62  NNPPSPNPTPRNDVVELPIFPLPLVLFPGSILPLQIFEFRYRIMMHTLLQTDLRFGVIFS 121
           NN P  NP+P+ND+VELPIFPLPLVLFPGSILPLQIFEFRYRIMMHTLLQTDLRFG+IF+
Sbjct: 63  NNLPPSNPSPQNDIVELPIFPLPLVLFPGSILPLQIFEFRYRIMMHTLLQTDLRFGIIFT 122

Query: 122 DTLSAAATSVGCVGEVVKHERLADDRFFLICKGQERFRINKIVRSKPYLVAAVDWLEDKP 181
           D+LS+  TS+GCVGEVVKHE+L+DDRFFLICKGQERFRINKIVRSKPYLVAAVDWLEDKP
Sbjct: 123 DSLSSTVTSIGCVGEVVKHEKLSDDRFFLICKGQERFRINKIVRSKPYLVAAVDWLEDKP 182

Query: 182 SSDGSHEDLESLAKDVESYMRDVIRLSNRLNGKEEKEVGDLRRNLFPTPFSFFVGSTFEG 241
           S DGSHEDL++LAKDVE YMRDVIRLSNRLNGKEEKEV DLRRNLFPTPFSFFVGSTFEG
Sbjct: 183 SIDGSHEDLDALAKDVEGYMRDVIRLSNRLNGKEEKEVADLRRNLFPTPFSFFVGSTFEG 242

Query: 242 APREQQALLELEDTAARLRREKETLRNTLNYLSAASAVKDVFPSSST 287
           APREQQALLELEDTAARL+REKETLRNTLNYLSAASAVKDVFPSSS+
Sbjct: 243 APREQQALLELEDTAARLKREKETLRNTLNYLSAASAVKDVFPSSSS 281

[Close]

BLAST of Spo22017.1 vs. UniProtKB/TrEMBL
Match: M0ZWD0_SOLTU (Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG400003704 PE=4 SV=1)

HSP 1 Score: 380.6 bits (976), Expect = 1.700e-102
Identity = 213/293 (72.70%), Postives = 239/293 (81.57%), Query Frame = 1


		  
Query: 1   MALPQFLHYSTTTITTPKPSLNPTP----PTFLHP-----SPLSLLHSHRKHHRNLRCSA 60
           MALPQ L  S ++  T KP LNP+     P F +P     S L L H  +++   +RCSA
Sbjct: 1   MALPQILP-SPSSSFTHKPHLNPSNFFLIPQFNNPQCQFSSKLLLQHRRQRNSNGIRCSA 60

Query: 61  SSSSPQPHNNPPSPNPTPRNDVVELPIFPLPLVLFPGSILPLQIFEFRYRIMMHTLLQTD 120
           SSS P+ H+   SP     +DVVELP+FPLPLVLFPG+ILPLQIFEFRYRIMMHTLLQTD
Sbjct: 61  SSSFPEKHHTG-SPKS---DDVVELPLFPLPLVLFPGAILPLQIFEFRYRIMMHTLLQTD 120

Query: 121 LRFGVIFSDTLSAAATSVGCVGEVVKHERLADDRFFLICKGQERFRINKIVRSKPYLVAA 180
           LRFGVI+SD+ +  A  VGCVGEVVKHERL DDRFFLICKGQERFR+NK+VRSKPYLVA 
Sbjct: 121 LRFGVIYSDSATGTA-DVGCVGEVVKHERLVDDRFFLICKGQERFRVNKLVRSKPYLVAE 180

Query: 181 VDWLEDKPSSDGSHEDLESLAKDVESYMRDVIRLSNRLNGKEEKEVGDLRRNLFPTPFSF 240
           V WLED+PS++G  +D+E LA +VESYM+DVIRLSNRLNGK EKE  DLRRNLFPTPFSF
Sbjct: 181 VTWLEDRPSANGE-DDVEGLANEVESYMKDVIRLSNRLNGKPEKEATDLRRNLFPTPFSF 240

Query: 241 FVGSTFEGAPREQQALLELEDTAARLRREKETLRNTLNYLSAASAVKDVFPSS 285
           FVGSTFEGAPREQQALLELEDTA RL+REKETLRNTLNYLSAASAVKDVFPSS
Sbjct: 241 FVGSTFEGAPREQQALLELEDTAMRLKREKETLRNTLNYLSAASAVKDVFPSS 286

[Close]

BLAST of Spo22017.1 vs. UniProtKB/TrEMBL
Match: A0A103XYM5_CYNCS (Uncharacterized protein OS=Cynara cardunculus var. scolymus GN=Ccrd_022445 PE=4 SV=1)

HSP 1 Score: 379.0 bits (972), Expect = 5.000e-102
Identity = 214/293 (73.04%), Postives = 239/293 (81.57%), Query Frame = 1


		  
Query: 1   MALPQFLHYSTTTITTPKPSLNPTPPTFLHPS---PLSLLHSHRKHHRN----LRCSASS 60
           MALPQ L   +++  T    LNP   +F  PS    +SL H  R+HHRN    LRCS+SS
Sbjct: 1   MALPQLLPSPSSSYLTHH-RLNPHTLSF-SPSNFNSISLPH-RRRHHRNHTSSLRCSSSS 60

Query: 61  SSPQPHNNPPSPNPTPRNDVVELPIFPLPLVLFPGSILPLQIFEFRYRIMMHTLLQTDLR 120
            S + H N PSPN    ND+VELP+FPLPLVLFPG+ILPLQIFE+RYRIMMHTLLQTDLR
Sbjct: 61  FS-EKHENTPSPNS---NDIVELPLFPLPLVLFPGAILPLQIFEYRYRIMMHTLLQTDLR 120

Query: 121 FGVIFSDTLSAAATSVGCVGEVVKHERLADDRFFLICKGQERFRINKIVRSKPYLVAAVD 180
           FGVI++D  S  A  VGCVGEVVKHERL DDRFF+ICKGQERFR+ K+VR+KPYLVA V 
Sbjct: 121 FGVIYTDATSGTA-DVGCVGEVVKHERLVDDRFFIICKGQERFRVTKVVRTKPYLVAEVV 180

Query: 181 WLEDKPSSDGSHEDLESLAKDVESYMRDVIRLSNRLNGKEEKEVGDLRRNLFPTPFSFFV 240
           WLED+PS +G  EDLE LA +VES+M+DVIRLSNRLNGK EKE GDLRRNLFPTPFSFFV
Sbjct: 181 WLEDRPSGNGE-EDLEGLASEVESHMKDVIRLSNRLNGKPEKEAGDLRRNLFPTPFSFFV 240

Query: 241 GSTFEGAPREQQALLELEDTAARLRREKETLRNTLNYLSAASAVKDVFPSSST 287
           GSTFEGAPREQQALLELEDT  RL+REKETLRNTLNYLSAASAVKDVFPSSS+
Sbjct: 241 GSTFEGAPREQQALLELEDTMVRLKREKETLRNTLNYLSAASAVKDVFPSSSS 284

[Close]

BLAST of Spo22017.1 vs. UniProtKB/TrEMBL
Match: A0A0V0HRH6_SOLCH (Putative LON peptidase N-terminal domain and RING finger protein 1-like OS=Solanum chacoense PE=4 SV=1)

HSP 1 Score: 379.0 bits (972), Expect = 5.000e-102
Identity = 213/293 (72.70%), Postives = 238/293 (81.23%), Query Frame = 1


		  
Query: 1   MALPQFLHYSTTTITTPKPSLNPTP----PTFLHP-----SPLSLLHSHRKHHRNLRCSA 60
           MALPQ L  S ++  T KP LNP+     P F +P     S L L    R++   +RCSA
Sbjct: 1   MALPQILP-SPSSSFTHKPHLNPSNFFLIPQFNNPQCQFSSKLLLQRRRRRNSNGIRCSA 60

Query: 61  SSSSPQPHNNPPSPNPTPRNDVVELPIFPLPLVLFPGSILPLQIFEFRYRIMMHTLLQTD 120
           SSS P+ H+   SP     +DVVELP+FPLPLVLFPG+ILPLQIFEFRYRIMMHTLLQTD
Sbjct: 61  SSSFPEKHHTG-SPKS---DDVVELPLFPLPLVLFPGAILPLQIFEFRYRIMMHTLLQTD 120

Query: 121 LRFGVIFSDTLSAAATSVGCVGEVVKHERLADDRFFLICKGQERFRINKIVRSKPYLVAA 180
           LRFGVI+SD+ +  A  VGCVGEVVKHERL DDRFFLICKGQERFR+NK+VRSKPYLVA 
Sbjct: 121 LRFGVIYSDSATGTA-DVGCVGEVVKHERLVDDRFFLICKGQERFRVNKLVRSKPYLVAE 180

Query: 181 VDWLEDKPSSDGSHEDLESLAKDVESYMRDVIRLSNRLNGKEEKEVGDLRRNLFPTPFSF 240
           V WLED+PS++G  +D+E LA +VESYM+DVIRLSNRLNGK EKE  DLRRNLFPTPFSF
Sbjct: 181 VTWLEDRPSANGE-DDVEGLANEVESYMKDVIRLSNRLNGKPEKEATDLRRNLFPTPFSF 240

Query: 241 FVGSTFEGAPREQQALLELEDTAARLRREKETLRNTLNYLSAASAVKDVFPSS 285
           FVGSTFEGAPREQQALLELEDTA RL+REKETLRNTLNYLSAASAVKDVFPSS
Sbjct: 241 FVGSTFEGAPREQQALLELEDTAMRLKREKETLRNTLNYLSAASAVKDVFPSS 286

[Close]

BLAST of Spo22017.1 vs. ExPASy Swiss-Prot
Match: LON2_MYXXA (Lon protease 2 OS=Myxococcus xanthus GN=lon2 PE=1 SV=3)

HSP 1 Score: 73.2 bits (178), Expect = 5.300e-12
Identity = 65/234 (27.78%), Postives = 115/234 (49.15%), Query Frame = 1


		  
Query: 52  SSSSPQPHNNPPSPNPTPRNDVVE-LPIFPLP-LVLFPGSILPLQIFEFRYRIMMHTLLQ 111
           S++S  P    P P    + D+ + LPI PL   V FPG +LPL +   +   ++   ++
Sbjct: 9   SAASAMPTAMAP-PGLINKEDIPQVLPILPLRNSVFFPGGVLPLAVGRQKTIALIKDAVR 68

Query: 112 TDLRFGVIFS------DTLSAAATSVGCVGEVVKHERLADDRFFLICKGQERFRINKIVR 171
            D   GV+        D  +A   ++G V  +VK  ++ +D + L+ +G  RFR+ ++V+
Sbjct: 69  DDQVIGVVTQRRAEEEDPGAADLYTMGTVARIVKLLKMGEDNYSLVVQGLARFRVVELVQ 128

Query: 172 SKPYLVAAVDWLEDKPSSDGSHEDLESLAKDVESYMRDVIRLSNRLNGKEEKEVGDLRRN 231
             PYL A VD +EDK SS+  + ++E+L  +++   R+VI L   L     + V  +   
Sbjct: 129 EAPYLKARVDAVEDKTSSE--NVEVEALGINLKKLAREVIELMPELPAAATELVESITH- 188

Query: 232 LFPTPFSFFVGSTFEGAPREQQALLELEDTAARLRREKETLRNTLNYLSAASAV 278
             P   +  + +  +    E+QA+LE  D  AR++   E L      L  ++ +
Sbjct: 189 --PGHLADLIAANVDVPIEEKQAVLETVDLKARMKLVLELLNRKREILKLSNKI 236

[Close]

BLAST of Spo22017.1 vs. ExPASy Swiss-Prot
Match: LON_ANADE (Lon protease OS=Anaeromyxobacter dehalogenans (strain 2CP-C) GN=lon PE=3 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 3.800e-10
Identity = 54/209 (25.84%), Postives = 101/209 (48.33%), Query Frame = 1


		  
Query: 76  LPIFPLP-LVLFPGSILPLQIFEFRYRIMMHTLLQTDLRFGVIFS------DTLSAAATS 135
           LPI PL   V FPG +LPL +   +   ++   ++ +   GV+        D  +A   +
Sbjct: 42  LPILPLRNSVFFPGGVLPLAVGRQKTIALIKDAVRDEQVIGVVTQRRAEEEDPGAADLYT 101

Query: 136 VGCVGEVVKHERLADDRFFLICKGQERFRINKIVRSKPYLVAAVDWLEDKPSSDGSHEDL 195
           VG V  VVK  ++ +D + L+ +G  RF++ ++V+  PYL A ++ +ED+   D    ++
Sbjct: 102 VGTVARVVKLLKMGEDNYSLVVQGLARFKVLELVQESPYLKARIEAVEDRSVVD--DVEV 161

Query: 196 ESLAKDVESYMRDVIRLSNRLNGKEEKEVGDLRRNLFPTPFSFFVGSTFEGAPREQQALL 255
           E+LA +++   R+VI L   L     + V  +     P   +  + +  +    E+Q +L
Sbjct: 162 EALAINLKKLAREVIELMPELPAAATELVESITH---PGHLADLIAANVDVPIEEKQQVL 221

Query: 256 ELEDTAARLRREKETLRNTLNYLSAASAV 278
           E  +  AR++   E L      L  ++ +
Sbjct: 222 ETVELKARMKLVLELLNRKREILKLSNKI 245

[Close]

BLAST of Spo22017.1 vs. ExPASy Swiss-Prot
Match: LON_CYTH3 (Lon protease OS=Cytophaga hutchinsonii (strain ATCC 33406 / NCIMB 9469) GN=lon PE=3 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 1.200e-8
Identity = 45/188 (23.94%), Postives = 93/188 (49.47%), Query Frame = 1


		  
Query: 76  LPIFPLP-LVLFPGSILPLQIFEFRYRIMMHTLLQTDLRFGVIFSDTLSAAATS------ 135
           LPI P+  +VLFPG +LP+ +   +   ++    + D   GV+  +   +   S      
Sbjct: 30  LPILPVRNIVLFPGVVLPITVGRQKSIRLVKKFYKGDRTIGVVAQENQKSEEPSFQDIFK 89

Query: 136 VGCVGEVVKHERLADDRFFLICKGQERFRINKIVRSKPYLVAAVDWLEDKPSSDGSHEDL 195
           VG V ++++   L D    +I +G+ RF+I + V+ +P++ A V  L+D    D S +++
Sbjct: 90  VGTVAKILRMFVLPDGNTTIIIQGKRRFKIEEQVQDEPFMQAKVSMLKD-IHPDMSKKEV 149

Query: 196 ESLAKDVESYMRDVIRLSNRLNGKEEKEVGDLRRNLFPTPFSFFVGSTFEGAPREQQALL 255
           ++L + V+     +++++  +    +  + ++    F T    F+ S      +++Q LL
Sbjct: 150 KALLQSVKESATKILKMNPEIPQDAQIAINNIESENFLT---HFLSSNINAELKDKQKLL 209

Query: 256 ELEDTAAR 257
           E +D   R
Sbjct: 210 EFDDAVER 213

[Close]

BLAST of Spo22017.1 vs. ExPASy Swiss-Prot
Match: LON_AMOA5 (Lon protease OS=Amoebophilus asiaticus (strain 5a2) GN=lon PE=3 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 1.300e-7
Identity = 44/180 (24.44%), Postives = 85/180 (47.22%), Query Frame = 1


		  
Query: 84  VLFPGSILPLQIFEFRYRIMMHTLLQTDLRFGVIFSDTLSAAATS------VGCVGEVVK 143
           VLFPG  +P+ +       ++  + +T    G++        ATS      +G    ++K
Sbjct: 47  VLFPGIYMPMTLENASIIRLVKKVYETGGIIGIVAQKKEDVEATSAQDIFTIGTTARILK 106

Query: 144 HERLADDRFFLICKGQERFRINKIVRSKPYLVAAVDWLEDKPSSDGSHEDLESLAKDVES 203
              L D+R  ++ +G+E+F+I  ++   PYL+A++  L+DK S+  S        K V S
Sbjct: 107 LINLPDERVRILLQGEEKFQIEDVIAETPYLLASISRLKDKTSNTQSKH-----FKAVVS 166

Query: 204 YMRDVIRLSNRLNGKEEKEVGDLRRNLFP-TPFSFFVGSTFEGAPREQQALLELEDTAAR 257
            +++ +     L  +   E+  L  N+      ++F+ S  +   + +Q LLE+ D+  R
Sbjct: 167 SIKETVAKLISLQPEFPTEIKLLLDNINDFNLLTYFLASGLDTDIKSKQKLLEIHDSKKR 221

[Close]

BLAST of Spo22017.1 vs. TAIR (Arabidopsis)
Match: AT1G75460.1 (ATP-dependent protease La (LON) domain protein)

HSP 1 Score: 354.8 bits (909), Expect = 5.100e-98
Identity = 195/287 (67.94%), Postives = 230/287 (80.14%), Query Frame = 1


		  
Query: 1   MALPQFLHYSTTTITTPKPSLNPTPPTFLHPSPLSLLHSHR--KHHRNLRCSASSSSPQP 60
           MALP  +  S+ + T+  PS    PP     S    + + R  K + + RCS+SS S + 
Sbjct: 1   MALPHVV--SSPSSTSLSPSFKSKPPFHSLRSLSPTVDNRRRCKLNSSFRCSSSSFS-EK 60

Query: 61  HNNPPSPNPTPRNDVVELPIFPLPLVLFPGSILPLQIFEFRYRIMMHTLLQTDLRFGVIF 120
           H+N  SP     +D+VELP+FPLPLVLFPG+ILPLQIFEFRYRIMMHTLLQ+DLRFGV++
Sbjct: 61  HHNAKSPKS---DDIVELPLFPLPLVLFPGAILPLQIFEFRYRIMMHTLLQSDLRFGVVY 120

Query: 121 SDTLSAAATSVGCVGEVVKHERLADDRFFLICKGQERFRINKIVRSKPYLVAAVDWLEDK 180
           SD++S +A  VGCVGEVVKHERL DDRFFL+CKGQERFR+  +VR+KPYLV  V WLED+
Sbjct: 121 SDSVSGSAAEVGCVGEVVKHERLVDDRFFLVCKGQERFRVTNVVRTKPYLVGEVTWLEDR 180

Query: 181 PSSDGSHEDLESLAKDVESYMRDVIRLSNRLNGKEEKEVGDLRRNLFPTPFSFFVGSTFE 240
           PS +   E+L+SLA +VE  M++VIRLSNRLNGK EKEV DLRRN FPTPFSFFVGSTFE
Sbjct: 181 PSGE---ENLDSLANEVEVLMKEVIRLSNRLNGKAEKEVQDLRRNQFPTPFSFFVGSTFE 240

Query: 241 GAPREQQALLELEDTAARLRREKETLRNTLNYLSAASAVKDVFPSSS 286
           GAPREQQALLELEDTAARL+RE+ETLRNTLNYL+AASAVKDVFPSSS
Sbjct: 241 GAPREQQALLELEDTAARLKRERETLRNTLNYLTAASAVKDVFPSSS 278

[Close]

BLAST of Spo22017.1 vs. TAIR (Arabidopsis)
Match: AT1G19740.1 (ATP-dependent protease La (LON) domain protein)

HSP 1 Score: 348.2 bits (892), Expect = 4.800e-96
Identity = 184/277 (66.43%), Postives = 220/277 (79.42%), Query Frame = 1


		  
Query: 15  TTPKPSLNPTPP--TFLHPSPLSLLHSHRKHHR------NLRCSASSSSPQPHNNPPSPN 74
           T   PS   TPP  +  +P PL L  ++R  +R      + RCS+SS S + HNN    N
Sbjct: 6   TVSSPSFKSTPPYLSAFNPKPLHLPATYRSINRRSCKLLSFRCSSSSFSEKHHNNA---N 65

Query: 75  PTPRNDVVELPIFPLPLVLFPGSILPLQIFEFRYRIMMHTLLQTDLRFGVIFSDTLSAAA 134
           P   +D+VELP+FPLPLVLFPG+ +PLQIFEFRYR+MM TLLQ+DLRFGV++SD +S +A
Sbjct: 66  PPKSDDLVELPLFPLPLVLFPGATIPLQIFEFRYRVMMQTLLQSDLRFGVVYSDAVSGSA 125

Query: 135 TSVGCVGEVVKHERLADDRFFLICKGQERFRINKIVRSKPYLVAAVDWLEDKPSSDGSHE 194
             +GCVGE+VKHERL DDRFFLICKGQERFR+  +VR+KPYLVA V WLED+PS +   E
Sbjct: 126 AGIGCVGEIVKHERLVDDRFFLICKGQERFRVTDLVRTKPYLVAKVTWLEDRPSGE---E 185

Query: 195 DLESLAKDVESYMRDVIRLSNRLNGKEEKEVGDLRRNLFPTPFSFFVGSTFEGAPREQQA 254
           +L+ LA +VE  M++VIRLSNRLNGK +KE  DLR+N FPTPFSFFVGSTFEGAP EQQA
Sbjct: 186 NLDELANEVEVLMKEVIRLSNRLNGKPDKESQDLRKNQFPTPFSFFVGSTFEGAPMEQQA 245

Query: 255 LLELEDTAARLRREKETLRNTLNYLSAASAVKDVFPS 284
           LLELEDTAARL+RE+ETLRNTLNYL+AASAVKDVFPS
Sbjct: 246 LLELEDTAARLKRERETLRNTLNYLTAASAVKDVFPS 276

The following BLAST results are available for this feature:

BLAST of Spo22017.1 vs. NCBI nr
BLAST of Spo22017.1 vs. UniProtKB/TrEMBL
BLAST of Spo22017.1 vs. ExPASy Swiss-Prot
BLAST of Spo22017.1 vs. TAIR (Arabidopsis)

BLAST of Spo22017.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|902141031\|gb\|KNA04675.1\|	4.6e-157	100.	hypothetical protein SOVF_1974...	[more]
gi\|731312083\|ref\|XP_010672533.1\|	2.7e-125	83.9	PREDICTED: uncharacterized pro...	[more]
gi\|565350052\|ref\|XP_006341988.1\|	2.5e-102	72.7	PREDICTED: lon protease 2-like...	[more]
gi\|976912871\|gb\|KVH99323.1\|	7.2e-102	73.0	hypothetical protein Ccrd_0224...	[more]
gi\|698558454\|ref\|XP_009771292.1\|	1.6e-101	72.4	PREDICTED: uncharacterized pro...	[more]

BLAST of Spo22017.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0K9QBP3_SPIOL	3.2e-157	100.	Uncharacterized protein OS=Spi...	[more]
A0A0J8FYF5_BETVU	1.9e-125	83.9	Uncharacterized protein OS=Bet...	[more]
M0ZWD0_SOLTU	1.7e-102	72.7	Uncharacterized protein OS=Sol...	[more]
A0A103XYM5_CYNCS	5.0e-102	73.0	Uncharacterized protein OS=Cyn...	[more]
A0A0V0HRH6_SOLCH	5.0e-102	72.7	Putative LON peptidase N-termi...	[more]

BLAST of Spo22017.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 4


Match Name	E-value	Identity	Description
LON2_MYXXA	5.3e-12	27.7	Lon protease 2 OS=Myxococcus x...	[more]
LON_ANADE	3.8e-10	25.8	Lon protease OS=Anaeromyxobact...	[more]
LON_CYTH3	1.2e-8	23.9	Lon protease OS=Cytophaga hutc...	[more]
LON_AMOA5	1.3e-7	24.4	Lon protease OS=Amoebophilus a...	[more]

BLAST of Spo22017.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 2


Match Name	E-value	Identity	Description
AT1G75460.1	5.1e-98	67.9	ATP-dependent protease La (LON...	[more]
AT1G19740.1	4.8e-96	66.4	ATP-dependent protease La (LON...	[more]

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003111	ATP-dependent protease La (LON), substrate-binding domain	PFAM	PF02190	LON_substr_bdg	coord: 75..264 score: 3.7
IPR003111	ATP-dependent protease La (LON), substrate-binding domain	SMART	SM00464	lon_5	coord: 75..266 score: 3.2
IPR015947	PUA-like domain	unknown	SSF88697	PUA domain-like	coord: 75..265 score: 4.72
None	No IPR available	unknown	Coil	Coil	coord: 188..215 score: -coord: 240..274 scor
None	No IPR available	PANTHER	PTHR23327	RING FINGER PROTEIN 127	coord: 70..282 score: 6.1E
None	No IPR available	PANTHER	PTHR23327:SF12	ATP-DEPENDENT PROTEASE LA DOMAIN-CONTAINING PROTEIN	coord: 70..282 score: 6.1E

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0004176	ATP-dependent peptidase activity