Spo22466.1 (mRNA) | SpinachBase

Overview


Name	Spo22466.1
Type	mRNA
Organism	Spinacia oleracea (Spinach)
Description	rRNA intron-encoded homing endonuclease
Location	SpoScf_26019 : 123 .. 1352 (+)
Sequence length	513

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAGGGCTCATGCGTGCCGGTGTCGATCGAGGAGTGCTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTATGAACTAATTCAGACTGTGAAACTGCGAATGGCTCATTAAATCAGTTATAGTTTGTTTGATGGTACCTGCTACTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCAACAAACCCCGACTTCTGGAAGGGACGCATTTATTAGATAAAAGGTCAACGCGGGCTTTTAGCCCGTTGCTCTGATGATTCATGATAACTCGACGGATCGCACGGCCTTTGCGCCGGCGACGCATCATTCAAATTTCTGCCCTATCAACTTTCGATGGTAGGATAGTGGCCTACCATGGTGGTGACGGGTGACGGAGAATTAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCAATCCTGACACGGGGAGGTAGTGACAATAAATAACAATACCGGGCTCATTGAGTCTGGTAATTGGAATGAGTACAATCTAAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCTAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTTGGACCTTGGGGTGGTACGACCGGTCCGCCTTTTGGTGTGCACCGGCCGTCTCGCCTCTTTCGCCGGCGATGCGCTCCTGGCCTTAATTGGCCGGGTCGTGCCTCCGGCACTGTTACTTTGAAGAAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGTATACATTAGCATGGGATAACATTATAGGATTCCGGTCCTATTGTGTTGGCCTTCGGGATCGGAGTAATGATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATGAAAGACGAATAACTGCGAAAGCATTTGCCAAGGATGTTTTCATTAATCAAGAACGAAAGTTGGGGGCTCGAAGACGATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCGGCGGATGTTACTTTTAGGACGCCGCCGGCACCTTATGAGATATCAAAGTTTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAA

mRNA sequence

ATGGGAGGGCTCATGCGTGCCGGTGTCGATCGAGGAGTGCTACCTGTTAAAAAGCTCGTAGTTGGACCTTGGGGTGGTACGACCGGTCCGCCTTTTGGTGTGCACCGGCCGTCTCGCCTCTTTCGCCGGCGATGCGCTCCTGGCCTTAATTGGCCGGGTCGTGCCTCCGGCACTGTTACTTTGAAGAAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGTATACATTAGCATGGGATAACATTATAGGATTCCGACGAATAACTGCGAAAGCATTTGCCAAGGATGTTTTCATTAATCAAGAACGAAAGTTGGGGGCTCGAAGACGATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCGGCGGATGTTACTTTTAGGACGCCGCCGGCACCTTATGAGATATCAAAGTTTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAA

Coding sequence (CDS)

ATGGGAGGGCTCATGCGTGCCGGTGTCGATCGAGGAGTGCTACCTGTTAAAAAGCTCGTAGTTGGACCTTGGGGTGGTACGACCGGTCCGCCTTTTGGTGTGCACCGGCCGTCTCGCCTCTTTCGCCGGCGATGCGCTCCTGGCCTTAATTGGCCGGGTCGTGCCTCCGGCACTGTTACTTTGAAGAAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGTATACATTAGCATGGGATAACATTATAGGATTCCGACGAATAACTGCGAAAGCATTTGCCAAGGATGTTTTCATTAATCAAGAACGAAAGTTGGGGGCTCGAAGACGATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCGGCGGATGTTACTTTTAGGACGCCGCCGGCACCTTATGAGATATCAAAGTTTTTGGGTTCCGGGGGGAGTATGGTCGCAAGGCTGAAACTTAAAGGAATTGACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTAA

Protein sequence

MGGLMRAGVDRGVLPVKKLVVGPWGGTTGPPFGVHRPSRLFRRRCAPGLNWPGRASGTVTLKKLECSKQAYALYTLAWDNIIGFRRITAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEISKFLGSGGSMVARLKLKGIDGRAPPGVEPAA

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Spo22466	Spo22466	gene

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Spo22466.1	Spo22466.1-protein	polypeptide

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo22466.1.exon.1	Spo22466.1.exon.1	exon
Spo22466.1.exon.2	Spo22466.1.exon.2	exon
Spo22466.1.exon.3	Spo22466.1.exon.3	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Spo22466.1.CDS.1	Spo22466.1.CDS.1	CDS
Spo22466.1.CDS.2	Spo22466.1.CDS.2	CDS
Spo22466.1.CDS.3	Spo22466.1.CDS.3	CDS

Homology

[Close]

BLAST of Spo22466.1 vs. NCBI nr
Match: gi|703137890|ref|XP_010106597.1| (hypothetical protein L484_002552 [Morus notabilis])

HSP 1 Score: 260.0 bits (663), Expect = 2.900e-66
Identity = 129/138 (93.48%), Postives = 130/138 (94.20%), Query Frame = 1


		  
Query: 33  GVHRPSRLFRRRCAPGLNWPGRASGTVTLKKLECSKQAYALYTLAWDNIIGFRRITAKAF 92
           GVHR +R F RRCAPGLNWPGRASG VTLKKLECSKQAYALYTLAWDNIIGFRR TAKAF
Sbjct: 55  GVHRSARPFCRRCAPGLNWPGRASGAVTLKKLECSKQAYALYTLAWDNIIGFRRTTAKAF 114

Query: 93  AKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEISKFLGSGGSMVAR 152
           AKDVFINQERKLGARRRSDTVLVSTINDADQGSADV FRTP APYE SKFLGSGGSMVAR
Sbjct: 115 AKDVFINQERKLGARRRSDTVLVSTINDADQGSADVAFRTPLAPYEKSKFLGSGGSMVAR 174

Query: 153 LKLKGIDGRAPPGVEPAA 171
           LKLKGIDGRAPPGVEPAA
Sbjct: 175 LKLKGIDGRAPPGVEPAA 192

[Close]

BLAST of Spo22466.1 vs. NCBI nr
Match: gi|823127874|ref|XP_012440607.1| (PREDICTED: uncharacterized protein LOC105765883 [Gossypium raimondii])

HSP 1 Score: 223.0 bits (567), Expect = 3.900e-55
Identity = 116/152 (76.32%), Postives = 121/152 (79.61%), Query Frame = 1


		  
Query: 16  VKKLVVGPWGGTTGPPFGVHRPSRLFRRRCAPGLNWPGRASGTVTLKKLECSKQAYALYT 75
           VKKLVVG  GG+ GPP G HR +R + RRCAPGLNWPGR+S  VTLKKLECSKQAYA   
Sbjct: 40  VKKLVVGLRGGSAGPPHGEHRSARPYCRRCAPGLNWPGRSSDAVTLKKLECSKQAYA--- 99

Query: 76  LAWDNIIGFRRITAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPA 135
                          +FAKDVFINQERKLGARRRSDTVLVSTINDADQGSADV FRTPPA
Sbjct: 100 ---------------SFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVAFRTPPA 159

Query: 136 PYEISKFLGSGGSMVARLKLKGIDGRAPPGVE 168
           PYE SK LGSGGSMVARLKLKGIDGRAPPGVE
Sbjct: 160 PYEKSKSLGSGGSMVARLKLKGIDGRAPPGVE 173

[Close]

BLAST of Spo22466.1 vs. NCBI nr
Match: gi|694380941|ref|XP_009366572.1| (PREDICTED: uncharacterized protein LOC103956333 [Pyrus x bretschneideri])

HSP 1 Score: 219.9 bits (559), Expect = 3.300e-54
Identity = 116/142 (81.69%), Postives = 119/142 (83.80%), Query Frame = 1


		  
Query: 33  GVHRPSRLFRRRCAPGLNWPGRASGTVTLKKLECSKQAYALYTLAWD-----NIIGFRRI 92
           GVHR +R F RR APGLNWPGRASG VTLKKLECSKQ +    LA+      N     R 
Sbjct: 228 GVHRSARPFCRRYAPGLNWPGRASGAVTLKKLECSKQDFGPILLAFGIGVMINRDSRGRT 287

Query: 93  TAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEISKFLGSGG 152
           TAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYE SKFLGSGG
Sbjct: 288 TAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEKSKFLGSGG 347

Query: 153 SMVARLKLKGIDGRAPPGVEPA 170
           SMVARLKLKGIDGRAPPGVEPA
Sbjct: 348 SMVARLKLKGIDGRAPPGVEPA 369

[Close]

BLAST of Spo22466.1 vs. NCBI nr
Match: gi|922327155|ref|XP_013442970.1| (hypothetical protein MTR_0055s0130 [Medicago truncatula])

HSP 1 Score: 213.8 bits (543), Expect = 2.400e-52
Identity = 114/143 (79.72%), Postives = 117/143 (81.82%), Query Frame = 1


		  
Query: 32  FGVHRPSRLFRRRCAPGLNWPGRASGTVTLKKLECSKQAYALYTLAWD-----NIIGFRR 91
           +GVHR +R F RRCAPGLNWPGRASG VTLKKLECSKQ      LA+      N     R
Sbjct: 485 YGVHRLARPFCRRCAPGLNWPGRASGAVTLKKLECSKQDSDPIVLAFGIGVMINRDSRGR 544

Query: 92  ITAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEISKFLGSG 151
            TAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADV FRTP APYE SK LGSG
Sbjct: 545 TTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVAFRTPLAPYEKSKSLGSG 604

Query: 152 GSMVARLKLKGIDGRAPPGVEPA 170
           GSMVARLKLKGIDGRAPPGVEPA
Sbjct: 605 GSMVARLKLKGIDGRAPPGVEPA 627

[Close]

BLAST of Spo22466.1 vs. NCBI nr
Match: gi|947068904|gb|KRH17795.1| (hypothetical protein GLYMA_13G016700 [Glycine max])

HSP 1 Score: 211.1 bits (536), Expect = 1.500e-51
Identity = 114/143 (79.72%), Postives = 116/143 (81.12%), Query Frame = 1


		  
Query: 33  GVHRPSRLFRRRCAPGLNWPGRASGTVTLKKLECSKQAYALYTLAWD-----NIIGFRRI 92
           GVHR +R F RRCAP LNWPGRASG VTLKKLECSKQ      LA+      N     R 
Sbjct: 128 GVHRSARPFCRRCAPVLNWPGRASGAVTLKKLECSKQDSDPIVLAFGIGVMINRDSRGRT 187

Query: 93  TAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEISKFLGSGG 152
           TAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADV FRTP APYE SK LGSGG
Sbjct: 188 TAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVAFRTPLAPYEKSKSLGSGG 247

Query: 153 SMVARLKLKGIDGRAPPGVEPAA 171
           SMVARLKLKGIDGRAPPGVEPAA
Sbjct: 248 SMVARLKLKGIDGRAPPGVEPAA 270

[Close]

BLAST of Spo22466.1 vs. UniProtKB/TrEMBL
Match: M1DUP9_SOLTU (Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG400044276 PE=4 SV=1)

HSP 1 Score: 263.1 bits (671), Expect = 2.400e-67
Identity = 131/141 (92.91%), Postives = 131/141 (92.91%), Query Frame = 1


		  
Query: 30  PPFGVHRPSRLFRRRCAPGLNWPGRASGTVTLKKLECSKQAYALYTLAWDNIIGFRRITA 89
           P   VHR SR F RRCAPGLNWPGRASG VTLKKLECSKQAYALYTLAWDNIIGFRR TA
Sbjct: 45  PCICVHRSSRPFCRRCAPGLNWPGRASGAVTLKKLECSKQAYALYTLAWDNIIGFRRTTA 104

Query: 90  KAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEISKFLGSGGSM 149
           KAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADV FRTPPAPYE SKFLGSGGSM
Sbjct: 105 KAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVAFRTPPAPYEKSKFLGSGGSM 164

Query: 150 VARLKLKGIDGRAPPGVEPAA 171
           VARLKLKGIDGRAPPGVEPAA
Sbjct: 165 VARLKLKGIDGRAPPGVEPAA 185

[Close]

BLAST of Spo22466.1 vs. UniProtKB/TrEMBL
Match: W9SJ11_9ROSA (Uncharacterized protein OS=Morus notabilis GN=L484_002552 PE=4 SV=1)

HSP 1 Score: 260.0 bits (663), Expect = 2.000e-66
Identity = 129/138 (93.48%), Postives = 130/138 (94.20%), Query Frame = 1


		  
Query: 33  GVHRPSRLFRRRCAPGLNWPGRASGTVTLKKLECSKQAYALYTLAWDNIIGFRRITAKAF 92
           GVHR +R F RRCAPGLNWPGRASG VTLKKLECSKQAYALYTLAWDNIIGFRR TAKAF
Sbjct: 55  GVHRSARPFCRRCAPGLNWPGRASGAVTLKKLECSKQAYALYTLAWDNIIGFRRTTAKAF 114

Query: 93  AKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEISKFLGSGGSMVAR 152
           AKDVFINQERKLGARRRSDTVLVSTINDADQGSADV FRTP APYE SKFLGSGGSMVAR
Sbjct: 115 AKDVFINQERKLGARRRSDTVLVSTINDADQGSADVAFRTPLAPYEKSKFLGSGGSMVAR 174

Query: 153 LKLKGIDGRAPPGVEPAA 171
           LKLKGIDGRAPPGVEPAA
Sbjct: 175 LKLKGIDGRAPPGVEPAA 192

[Close]

BLAST of Spo22466.1 vs. UniProtKB/TrEMBL
Match: M1DB49_SOLTU (Uncharacterized protein OS=Solanum tuberosum GN=PGSC0003DMG400035737 PE=4 SV=1)

HSP 1 Score: 247.3 bits (630), Expect = 1.400e-62
Identity = 122/130 (93.85%), Postives = 124/130 (95.38%), Query Frame = 1


		  
Query: 41  FRRRCAPGLNWPGRASGTVTLKKLECSKQAYALYTLAWDNIIGFRRITAKAFAKDVFINQ 100
           F RRCAPGLNWPGRASG VTLKKLECSKQAYALYTLAWDNIIGFRR TAKAFAKDVFINQ
Sbjct: 5   FCRRCAPGLNWPGRASGAVTLKKLECSKQAYALYTLAWDNIIGFRRTTAKAFAKDVFINQ 64

Query: 101 ERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEISKFLGSGGSMVARLKLKGIDG 160
           ERKLGARRRSDTVLVSTINDADQGSADV FRTPPAPYE SKFLGSGGS+VARLKLKGIDG
Sbjct: 65  ERKLGARRRSDTVLVSTINDADQGSADVAFRTPPAPYEKSKFLGSGGSIVARLKLKGIDG 124

Query: 161 RAPPGVEPAA 171
           +APPGVE AA
Sbjct: 125 KAPPGVELAA 134

[Close]

BLAST of Spo22466.1 vs. UniProtKB/TrEMBL
Match: A0A0D2ZQ67_BRAOL (Uncharacterized protein OS=Brassica oleracea var. oleracea PE=4 SV=1)

HSP 1 Score: 232.6 bits (592), Expect = 3.500e-58
Identity = 119/142 (83.80%), Postives = 123/142 (86.62%), Query Frame = 1


		  
Query: 33  GVHRPSRLFRRRCAPGLNWPGRASGTVTLKKLECSKQAYALYTLAWDNIIGFRRITA--- 92
           G+HR +  F R+ APGLNWPGRASG VTLKKLECSKQAY LYTLAWDNIIGF    A   
Sbjct: 189 GMHRSACPFCRQYAPGLNWPGRASGAVTLKKLECSKQAYTLYTLAWDNIIGFLSYCAGLQ 248

Query: 93  -KAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEISKFLGSGGS 152
            + FAKDVFINQERKLGARRRSDTVLVSTINDADQGSADV FRTP APYE SKFLGSGGS
Sbjct: 249 DRTFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVAFRTPLAPYEKSKFLGSGGS 308

Query: 153 MVARLKLKGIDGRAPPGVEPAA 171
           MVARLKLKGIDGRAPPGVEPAA
Sbjct: 309 MVARLKLKGIDGRAPPGVEPAA 330

[Close]

BLAST of Spo22466.1 vs. UniProtKB/TrEMBL
Match: A0A072TTW7_MEDTR (Uncharacterized protein OS=Medicago truncatula GN=MTR_0055s0130 PE=4 SV=1)

HSP 1 Score: 213.8 bits (543), Expect = 1.700e-52
Identity = 114/143 (79.72%), Postives = 117/143 (81.82%), Query Frame = 1


		  
Query: 32  FGVHRPSRLFRRRCAPGLNWPGRASGTVTLKKLECSKQAYALYTLAWD-----NIIGFRR 91
           +GVHR +R F RRCAPGLNWPGRASG VTLKKLECSKQ      LA+      N     R
Sbjct: 485 YGVHRLARPFCRRCAPGLNWPGRASGAVTLKKLECSKQDSDPIVLAFGIGVMINRDSRGR 544

Query: 92  ITAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVTFRTPPAPYEISKFLGSG 151
            TAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADV FRTP APYE SK LGSG
Sbjct: 545 TTAKAFAKDVFINQERKLGARRRSDTVLVSTINDADQGSADVAFRTPLAPYEKSKSLGSG 604

Query: 152 GSMVARLKLKGIDGRAPPGVEPA 170
           GSMVARLKLKGIDGRAPPGVEPA
Sbjct: 605 GSMVARLKLKGIDGRAPPGVEPA 627

The following BLAST results are available for this feature:

BLAST of Spo22466.1 vs. NCBI nr
BLAST of Spo22466.1 vs. UniProtKB/TrEMBL
BLAST of Spo22466.1 vs. ExPASy Swiss-Prot
BLAST of Spo22466.1 vs. TAIR (Arabidopsis)

BLAST of Spo22466.1 vs. NCBI nr
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. NCBI nr)
Total hits: 5


Match Name	E-value	Identity	Description
gi\|703137890\|ref\|XP_010106597.1\|	2.9e-66	93.4	hypothetical protein L484_0025...	[more]
gi\|823127874\|ref\|XP_012440607.1\|	3.9e-55	76.3	PREDICTED: uncharacterized pro...	[more]
gi\|694380941\|ref\|XP_009366572.1\|	3.3e-54	81.6	PREDICTED: uncharacterized pro...	[more]
gi\|922327155\|ref\|XP_013442970.1\|	2.4e-52	79.7	hypothetical protein MTR_0055s...	[more]
gi\|947068904\|gb\|KRH17795.1\|	1.5e-51	79.7	hypothetical protein GLYMA_13G...	[more]

BLAST of Spo22466.1 vs. UniProtKB/TrEMBL
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. UniprotKB/TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
M1DUP9_SOLTU	2.4e-67	92.9	Uncharacterized protein OS=Sol...	[more]
W9SJ11_9ROSA	2.0e-66	93.4	Uncharacterized protein OS=Mor...	[more]
M1DB49_SOLTU	1.4e-62	93.8	Uncharacterized protein OS=Sol...	[more]
A0A0D2ZQ67_BRAOL	3.5e-58	83.8	Uncharacterized protein OS=Bra...	[more]
A0A072TTW7_MEDTR	1.7e-52	79.7	Uncharacterized protein OS=Med...	[more]

BLAST of Spo22466.1 vs. ExPASy Swiss-Prot
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. ExPASy SwissProt)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of Spo22466.1 vs. TAIR (Arabidopsis)
Analysis Date: 2018-06-29 (blastp Spinacia oleracea peptides vs. TAIR)
Total hits: 0


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of S. oleracea
Date Performed: 2018-06-29


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR34410	FAMILY NOT NAMED	coord: 75..170 score: 1.8
None	No IPR available	PANTHER	PTHR34410:SF2	SUBFAMILY NOT NAMED	coord: 75..170 score: 1.8

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006506	GPI anchor biosynthetic process
biological_process	GO:0090305	nucleic acid phosphodiester bond hydrolysis
molecular_function	GO:0004519	endonuclease activity
molecular_function	GO:0016758	transferase activity, transferring hexosyl groups